SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS17015 HSERO_RS17015 sulfurtransferase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P27867 Sorbitol dehydrogenase; SDH; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.14; EC 1.1.1.9 from Rattus norvegicus (Rat) (see paper)
41% identity, 99% coverage: 3:342/345 of query aligns to 11:351/357 of P27867

query
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P27867

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C45 (= C38) binding
H70 (= H63) binding
E71 (= E64) binding
E156 (= E149) binding
D204 (= D197) binding
R209 (≠ K202) binding
VGM 273:275 (= VGM 264:266) binding
VFR 297:299 (= VFR 288:290) binding

3qe3A Sheep liver sorbitol dehydrogenase (see paper)
40% identity, 99% coverage: 3:342/345 of query aligns to 5:345/351 of 3qe3A

query
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3qe3A

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active site: C39 (= C38), G40 (= G39), S41 (= S40), H44 (= H43), H64 (= H63), E65 (= E64), R94 (= R93), D97 (≠ S96), C100 (≠ T99), S108 (≠ D107), F112 (≠ R111), P151 (= P150), G155 (= G154), K339 (≠ D336)
binding glycerol: Y45 (= Y44), F54 (= F53), T116 (= T115), R293 (= R290)
binding zinc ion: C39 (= C38), H64 (= H63), E65 (= E64)

1pl6A Human sdh/nadh/inhibitor complex (see paper)
41% identity, 96% coverage: 3:332/345 of query aligns to 10:340/356 of 1pl6A

query
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active site: C44 (= C38), G45 (= G39), S46 (= S40), H49 (= H43), H69 (= H63), E70 (= E64), R99 (= R93), D102 (≠ S96), C105 (≠ T99), S113 (≠ D107), F117 (≠ R111), P156 (= P150), G160 (= G154)
binding 4-[2-(hydroxymethyl)pyrimidin-4-yl]-n,n-dimethylpiperazine-1-sulfonamide: C44 (= C38), S46 (= S40), I56 (= I50), F59 (= F53), H69 (= H63), F118 (= F112), T121 (= T115), E155 (= E149), L274 (≠ M266), F297 (= F289), R298 (= R290)
binding nicotinamide-adenine-dinucleotide: V159 (≠ I153), G179 (= G173), A180 (= A174), G181 (= G175), P182 (≠ T176), I183 (= I177), T202 (≠ A196), D203 (= D197), L204 (≠ V198), R208 (≠ K202), I223 (≠ V217), C249 (≠ A241), T250 (≠ S242), A252 (vs. gap), V272 (= V264), G273 (= G265), L274 (≠ M266), V296 (= V288), F297 (= F289), R298 (= R290)
binding zinc ion: C44 (= C38), H69 (= H63)

Sites not aligning to the query:

active site: 344

Q00796 Sorbitol dehydrogenase; SDH; (R,R)-butanediol dehydrogenase; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Ribitol dehydrogenase; RDH; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.4; EC 1.1.1.14; EC 1.1.1.56; EC 1.1.1.9 from Homo sapiens (Human) (see 10 papers)
41% identity, 96% coverage: 3:332/345 of query aligns to 11:341/357 of Q00796

query
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Q00796

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C

Y

Y

R

K

L

L

P

P

D

D

N

N

V

V

S

T

F

F

A

E

E

E

G

G

A
|
A

I

L

V

I

E

E

P

P

L

L

S

S

I

V

G

G

L

I

Q

H

A

A

A

C

T

R

K

R

A

G

R

G

M

V

K

T

P

L

G

G

D

H

T

K

A

V

V

L

V

V

I

C

G

G

A

A

G

G

T

P

I
|
I

G

G

A

M

M

V

T

T

A

L

L

L

A

V

A

A

L

K

A

A

G

M

G

G

A

A

R

Q

V

V

I

V

L

V

A

T

D
|
D

V

L

V

S

A

A

E

T

K
x
R

L

L

A

S

H

K

F

A

A

K

D

E

N

I

P

G

A

A

V

D

I

L

T

V

V

L

D

Q

V

I

T

S

R

K

E

E

T

S

L

P

T

Q

D

E

V

I

V

A

R

R

Q

K

A

V

T

E

D

G

G
x
Q

W

L

G

G

A

C

-

K

-

P

D

E

V

V

V

T

F

I

E

E

A

C

S

T

G

G

-

A

-

E

-

A

-

S

-

I

H

Q

A

A

G

G

V

I

Y

Y

Q

A

T

T

L

R

L

-

D

-

L

-

V

-

C

-

P

S

G

G

C
x
N

A

L

V

V

L

L

V
|
V

G
|
G

M
x
L

P

G

P

S

A

E

P

M

V

T

A

T

L

V

D

P

V

L

A

H

M

A

Q

A

T

I

K

R

E

E

V

V

R

D

L

I

E

K

S

G

V
|
V

F
|
F

R
|
R

Y

Y

A

C

N

N

I

T

F

W

P

P

R

V

A

A

L

I

A

S

L

M

I

L

S

A

S

S

G

K

M

S

I

V

D

N

V

V

K

K

P

P

F

L

I
x
V

S

T

R

H

K

R

F

F

P

P

F

L

S

E

Q

K

S

A

I

L

R

E

A

A

F

F

E

E

E

T

A

F

A

K

S

K

G

G

C45 (= C38) binding
H70 (= H63) binding
E71 (= E64) binding
R110 (≠ M103) to P: in SORDD; results in protein aggregation
H135 (= H128) to R: in SORDD; results in protein aggregation
A153 (= A146) to D: in SORDD; unknown pathological significance; results in protein aggregation; dbSNP:rs145813597
I184 (= I177) binding
D204 (= D197) binding
R209 (≠ K202) binding
Q239 (≠ G232) to L: in dbSNP:rs1042079
N269 (≠ C260) to T: in dbSNP:rs930337
VGL 273:275 (≠ VGM 264:266) binding
VFR 297:299 (= VFR 288:290) binding
V322 (≠ I313) to I: in SORDD; unknown pathological significance; dbSNP:rs149975952

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P07846 Sorbitol dehydrogenase; SDH; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.14; EC 1.1.1.9 from Ovis aries (Sheep) (see paper)
39% identity, 99% coverage: 3:342/345 of query aligns to 9:348/354 of P07846

query
sites
P07846

A

S

L

L

V

V

L

V

E

H

A

G

T

P

R

G

E

D

L

L

K

R

L

L

R

E

E

N

I

Y

D

P

L

I

P

P

Q

E

Q

P

M

-

G

G

A

P

Q

N

D

E

V

V

R

L

I

L

R

K

I

M

H

H

T

S

V

V

G

G

I

I

C
|
C

G

G

S

S

D

D

L

V

H

H

Y
|
Y

Y

W

T

-

H

Q

G

G

S

R

I

I

G

G

P

D

F

F

K

V

V

V

E

K

A

K

P

P

M

M

V

V

L

L

G

G

H
|
H

E
|
E

A

A

S

S

G

G

T

T

V

V

I

V

E

K

V

V

G

G

S

S

A

L

V

V

S

R

H

H

L

L

K

Q

V

P

G

G

D

D

R

R

V

V

C

A

M

I

E

Q

P

P

G

G

I

A

P

P

R

R

L

Q

D

T

S

D

P

E

A

F

T

C

L

K

R

I

G

G

M

R

Y

Y

N

N

L

L

D

S

P

P

A

T

V

I

R

F

F

F

W

C

A

A

T

T

P

P

I

D

H

D

G

G

C

N

L

L

T

C

G

R

S

F

V

Y

V

K

H

H

P

N

A

A

A

N

F

F

T

C

Y

Y

R

K

L

L

P

P

D

D

N

N

V

V

S

T

F

F

A

E

E

E

G

G

A

A

I

L

V

I

E
|
E

P

P

L

L

S

S

I

V

G

G

L

I

Q

H

A

A

A

C

T

R

K

R

A

A

R

G

M

V

K

T

P

L

G

G

D

N

T

K

A

V

V

L

V

V

I

C

G

G

A

A

G

G

T

P

I

I

G

G

A

L

M

V

T

N

A

L

L

L

A

A

L

K

A

A

G

M

G

G

A

A

R

Q

V

V

I

V

L

V

A

T

D

D

V

L

V

S

A

A

E

S

K

R

L

L

A

S

H

K

F

A

A

K

D

E

N

V

P

G

A

A

V

D

I

F

T

I

V

L

D

E

V

I

T

S

R

N

E

E

T

S

L

P

T

E

D

E

V

I

V

A

R

K

Q

K

A

V

T

E

D

G

G

L

W

L

G

G

A

S

-

K

-

P

D

E

V

V

V

T

F

I

E

E

A

C

S

T

G

G

-

V

-

E

-

T

-

S

-

I

H

Q

A

A

G

G

V

I

Y

Y

Q

A

T

T

L

H

L

-

D

-

L

-

V

-

C

-

P

S

G

G

C

T

A

L

V

V

L

L

V

V

G

G

M

L

P

G

P

S

A

E

P

M

V

T

A

S

L

V

D

P

V

L

V

V

A

H

M

A

Q

A

T

T

K

R

E

E

V

V

R

D

L

I

E

K

S

G

V

V

F

F

R
|
R

Y
|
Y

A

C

N

N

I

T

F

W

P

P

R

M

A

A

L

I

A

S

L

M

I

L

S

A

S

S

G

K

M

S

I

V

D

N

V

V

K

K

P

P

F

L

I

V

S

T

R

H

K

R

F

F

P

P

F

L

S

E

Q

K

S

A

I

L

R

E

A

A

F

F

E

E

E

T

A

S

A

K

S

K

G

G

R

L

P

G

Q

L

D

K

V

V

K

M

I

I

Q

K

I

C

E

D

C43 (= C38) binding
Y49 (= Y44) binding
H67 (= H63) binding
E68 (= E64) binding
E153 (= E149) binding
R296 (= R290) binding
Y297 (= Y291) binding

1e3jA Ketose reductase (sorbitol dehydrogenase) from silverleaf whitefly (see paper)
39% identity, 98% coverage: 5:341/345 of query aligns to 6:344/348 of 1e3jA

query
sites
1e3jA

V

V

L

L

E

Y

A

K

T

Q

R

N

E

D

L

L

K

R

L

L

R

E

E

Q

I

R

D

P

L

I

P

P

Q

E

Q

P

M

K

G

-

A

E

Q

D

D

E

V

V

R

L

I

L

R

Q

I

M

H

A

T

Y

V

V

G

G

I

I

C
|
C

G
|
G

S
|
S

D

D

L

V

H
|
H

Y

Y

T

E

H

H

G

G

S

R

I

I

G

A

P

D

F

F

K

I

V

V

E

K

A

D

P

P

M

M

V

V

L

I

G

G

H
|
H

E
|
E

A

A

S

S

G

G

T

T

V

V

I

V

E

K

V

V

G

G

S

K

A

N

V

V

S

K

H

H

L

L

K

K

V

K

G

G

D

D

R

R

V

V

C

A

M

V

E

E

P

P

G

G

I

V

P

P

R
x
C

L

R

D

R

S
x
C

P

Q

A

F

T
x
C

L

K

R

E

G

G

M

K

Y

Y

N

N

L

L

D
x
C

P

P

A

D

V

L

R
x
T

F

F

W

C

A

A

T

T

P

P

I

D

H

D

G

G

C

N

L

L

T

A

G

R

S

Y

V

Y

V

V

H

H

P

A

A

A

A

D

F

F

T

C

Y

H

R

K

L

L

P

P

D

D

N

N

V

V

S

S

F

L

A

E

E

E

G

G

A

A

I

L

V

L

E

E

P
|
P

L

L

S

S

I

V

G
|
G

L

V

Q

H

A

A

A

C

T

R

K

R

A

A

R

G

M

V

K

Q

P

L

G

G

D

T

T

T

A

V

V

L

V

V

I

I

G
|
G

A
|
A

G

G

T

P

I

I

G

G

A

L

M

V

T

S

A

V

L

L

A

A

L

K

A

A

G

Y

G

G

A

A

A

F

R

V

V

V

I

C

L

T

A
|
A

D
x
R

V
x
S

V

P

A

R

E
x
R

-

L

K

E

L

V

A

A

H

K

F

N

A

C

D

G

N

A

P

D

A

V

V

T

I

L

T

V

V

V

D

D

V

P

T

A

R

K

E

E

T

E

L

E

T

S

D

S

V

I

R

E

Q

R

A

I

T

R

D

S

G

A

W

I

G

G

A

D

-

L

-

P

D

N

V

V

V

T

F

I

E

D

A

C

S

S

G

G

H

N

A

E

G

K

V

C

Y

I

Q

T

T

I

L

G

L

I

D

N

L

I

V

T

C

R

P

T

G

G

C

T

A

L

V

M

L

L

V

V

G

G

M

M

P

G

P

S

A

Q

P

M

V

V

A

T

L

V

D

P

V

L

V

V

A

N

M

A

Q

C

T

A

K

R

E

E

V

I

R

D

L

I

E

K

S

S

V

V

F

F

R

R

Y

Y

A

C

N

N

I

D

F

Y

P

P

R

I

A

A

L

L

A

E

L

M

I

V

S

A

S

S

G

G

M

R

I

C

D

N

V

V

K

K

P

Q

F

L

I

V

S

T

R

H

K

S

F

F

P

K

F

L

S

E

Q

Q

S

T

I

V

R

D

A

A

F

F

E

E

E

A

A

A

A

R

S

K

G

K

R

A

P

D

Q

N

D

T

V

I

K
|
K

I

V

Q

M

I

I

active site: C38 (= C38), G39 (= G39), S40 (= S40), H43 (= H43), H63 (= H63), E64 (= E64), C93 (≠ R93), C96 (≠ S96), C99 (≠ T99), C107 (≠ D107), T111 (≠ R111), P150 (= P150), G154 (= G154), K341 (= K338)
binding phosphate ion: G173 (= G173), A174 (= A174), A196 (= A196), R197 (≠ D197), S198 (≠ V198), R201 (≠ E201)
binding zinc ion: C38 (= C38), H63 (= H63), E64 (= E64), C93 (≠ R93), C96 (≠ S96), C99 (≠ T99), C107 (≠ D107)

5vm2A Crystal structure of eck1772, an oxidoreductase/dehydrogenase of unknown specificity involved in membrane biogenesis from escherichia coli
38% identity, 97% coverage: 4:338/345 of query aligns to 8:340/347 of 5vm2A

query
sites
5vm2A

L

L

V

Q

L

V

E

P

A

G

T

T

R

M

E

K

L

I

K

I

L

S

R

A

E

E

I

I

D

P

L

V

P

P

Q

K

Q

E

M

-

G

-

A

-

Q

D

D

E

V

V

R

L

I

I

R

K

I

V

H

E

T

Y

V

V

G

G

I

I

C
|
C

G
|
G

S
|
S

D

D

L

V

H
|
H

Y

G

Y

F

T

E

H

S

G

G

S

P

-

F

I

I

G

P

P

P

F

K

K

D

V

P

E

N

A

Q

P

E

M

I

V

G

L

L

G

G

H
|
H

E
|
E

A

C

S

A

G

G

T

T

V

V

I

V

E

A

V

V

G

G

S

S

A

R

V

V

S

R

H

K

L

F

K

K

V

P

G

G

D

D

R

R

V

V

C

N

M

I

E

E

P

P

G

G

I

V

P

P

R
x
C

L

G

D

H

S
x
C

P

R

A

Y

T
x
C

L

L

R

E

G

G

M

K

Y

Y

N

N

L

I

D
x
C

P

P

A

D

V

V

R
x
D

F

F

W

M

A

A

T

T

P

Q

P

P

I

N

H

Y

-

R

G

G

C

A

L

L

T

T

G

H

S

Y

V

L

V

C

H

H

P

P

A

E

A

S

F

F

T

T

Y

Y

R

K

L

L

P

P

D

D

N

N

V

M

S

D

F

T

A

M

E

E

G

G

A

T

I

L

V

V

E
|
E

P
|
P

L

A

S

A

I

V

G
|
G

L

M

Q

H

A

A

T

M

K

L

A

A

R

D

M

V

K

K

P

P

G

G

D

K

T

K

A

I

V

I

I

L

G

G

A

A

G

G

T

C

I

I

G

G

A

L

M

M

T

T

A

L

L

Q

A

A

A

C

L

K

A

C

G

L

G

G

A

A

A

T

R

E

V

I

I

A

L

V

A

V

D

D

V

V

V

L

A

E

E

K

K

R

L

L

A

A

H

M

F

A

A

E

D

Q

N

L

P

G

A

A

V

T

I

V

T

V

V

I

D

N

V

G

T

A

R

K

E

E

T

D

L

T

T

I

D

A

V

R

V

C

R

Q

Q

Q

A

F

T

T

D

E

G

D

W

M

G

G

A

A

D

D

V

I

V

V

F

F

E

E

A

T

S

A

G

G

H

S

A

A

G

V

V

T

Y

V

Q

K

T

Q

L

A

L

P

D

Y

L

L

V

V

C

M

P

R

G

G

C

K

A

I

V

M

L

I

V

V

G

G

M

T

P

V

P

P

A

G

P

A

V

S

A

A

L

I

D

N

V

F

V

L

A

K

M

I

Q

N

T

-

K

R

E

E

V

V

R

T

L

I

E

Q

S

T

V

V

F

F

R

R

Y

Y

A

A

N

N

I

R

F

Y

P

P

R

V

A

T

L

I

A

E

L

A

I

I

S

S

G

G

M

R

I

F

D

D

V

V

K

K

P

S

F

M

I

V

S

T

R

H

K

I

F

Y

P

D

F

Y

S

R

Q

D

S

V

I

Q

R

Q

A

A

F

F

E

E

A

S

A

V

S

N

G

N

R

K

P

R

Q

D

D

I

V

I

K
|
K

active site: C39 (= C38), G40 (= G39), S41 (= S40), H44 (= H43), H65 (= H63), E66 (= E64), C95 (≠ R93), C98 (≠ S96), C101 (≠ T99), C109 (≠ D107), D113 (≠ R111), P153 (= P150), G157 (= G154), K340 (= K338)
binding magnesium ion: H65 (= H63), E66 (= E64), E152 (= E149)
binding zinc ion: C95 (≠ R93), C98 (≠ S96), C101 (≠ T99), C109 (≠ D107)

Q7SI09 L-arabinitol 4-dehydrogenase; LAD; EC 1.1.1.12 from Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) (see 2 papers)
33% identity, 95% coverage: 14:342/345 of query aligns to 29:360/363 of Q7SI09

query
sites
Q7SI09

L

L

R

E

E

S

I

V

D

Q

L

K

P

G

Q

E

M

L

G

K

A

E

Q

G

D

E

V

V

R

T

I

V

R

A

I

V

H

R

T

S

V

T

G

G

I

I

C
|
C

G

G

S

S

D

D

L

V

H

H

Y
x
F

Y

W

T

K

H

H

G

G

S

C

I

I

G

G

P

P

F

M

K

I

V

V

E

E

A

C

P

D

M

H

V

V

L

L

G

G

H
|
H

E
|
E

A

S

S

A

G

G

T

E

V

V

I

I

E

A

V

V

G

H

S

P

A

S

V

V

S

K

H

S

L

I

K

K

V

V

G

G

D

D

R

R

V

V

C

A

M

I

E

E

P

P

G

Q

I

V

P

I

R
x
C

L

N

D

A

S
x
C

P

E

A

P

T
x
C

L

L

R

T

G

G

M

R

Y

Y

N

N

L

G

D
x
C

P

E

A

R

V

V

R

D

F

F

W

L

A

S

T

T

P

P

I

V

H

P

G

G

C

L

L

L

T

R

G

R

S

Y

V

V

V

N

H

H

P

P

A

A

A

V

F

W

T

C

Y

H

R

K

L

I

P

-

D

G

N

N

V

M

S

S

F

Y

A

E

E

N

G

G

A

A

I

M

V

L

E
|
E

P

P

L

L

S

S

I

V

G

A

L

L

Q

A

A

G

A

L

T

Q

K

R

A

A

R

G

M

V

K

R

P

L

G

G

D

D

T

P

A

V

V

L

V

I

I

C

G

G

A

A

G

G

T
x
P

I
|
I

G

G

A

L

M

I

T

T

A

M

L

L

A

C

A

A

L

K

A

A

G

A

G

G

A

A

A

C

R

P

V

L

I

V

L

I

A

T

D
|
D

V
x
I

V

D

A

E

E

G

K
x
R

L

L

A

-

H

K

F

F

A

A

D

K

N

E

-

I

-

C

P

P

A

E

V

V

I

V

T

T

V

H

D

K

V

V

T

E

R

R

E

L

T

S

L

A

T

E

D

E

V

S

V

A

R

K

Q

K

A

I

T

V

D

E

G

S

W

F

G

G

A

G

-

I

-

E

-

P

D

A

V

V

V

A

F

L

E

E

A

C

S

T

G

G

H

V

A

E

G

S

V

S

Y

I

Q

A

T

A

L

A

L

I

D

W

L

A

V

V

C

K

P

F

G

G

C

K

A

V

V

F

L

V

V
x
I

G

G

M

V

P

G

P

K

A

N

P

E

V

I

A

Q

L

I

D

P

V

F

V

M

A

R

M

A

Q

S

T

V

K

R

E

E

V

V

R

D

L

L

E

Q

S

F

V
x
Q

F
x
Y

R
|
R

Y

Y

A

C

N

N

I

T

F

W

P

P

R

R

A

A

L

I

A

R

L

L

I

V

S

E

S

N

G

G

M

L

I

V

D

D

V

L

K

T

P

R

F

L

I

V

S

T

R

H

K

R

F

F

P

P

F

L

S

E

Q

D

S

A

I

L

R

K

A

A

F

F

E

E

E

T

A

A

A
x
S

S

D

G

P

R

K

P

T

Q

G

D

A

V

I

K

K

I

V

Q

Q

I

I

E

Q

C53 (= C38) binding
F59 (≠ Y44) mutation F->A,S,Y: No effect.
H78 (= H63) binding
E79 (= E64) binding
C108 (≠ R93) binding
C111 (≠ S96) binding
C114 (≠ T99) binding
C122 (≠ D107) binding
E163 (= E149) binding
PI 190:191 (≠ TI 176:177) binding
D211 (= D197) binding
DI 211:212 (≠ DV 197:198) mutation to SR: Alters cofactor specificity from NAD to NADP; when associated with T-348.
R216 (≠ K202) binding
I282 (≠ V264) binding
QYR 306:308 (≠ VFR 288:290) binding
S348 (≠ A330) mutation to T: Alters cofactor specificity from NAD to NADP; when associated with 211-SR-212.

3m6iA L-arabinitol 4-dehydrogenase (see paper)
33% identity, 95% coverage: 14:342/345 of query aligns to 25:356/358 of 3m6iA

query
sites
3m6iA

L

L

R

E

E

S

I

V

D

Q

L

K

P

G

Q

E

M

L

G

K

A

E

Q

G

D

E

V

V

R

T

I

V

R

A

I

V

H

R

T

S

V

T

G

G

I

I

C
|
C

G
|
G

S
|
S

D

D

L

V

H
|
H

Y

F

Y

W

T

K

H

H

G

G

S

C

I

I

G

G

P

P

F

M

K

I

V

V

E

E

A

C

P

D

M

H

V

V

L

L

G

G

H
|
H

E
|
E

A

S

S

A

G

G

T

E

V

V

I

I

E

A

V

V

G

H

S

P

A

S

V

V

S

K

H

S

L

I

K

K

V

V

G

G

D

D

R

R

V

V

C

A

M

I

E

E

P

P

G

Q

I

V

P

I

R
x
C

L

N

D

A

S
x
C

P

E

A

P

T
x
C

L

L

R

T

G

G

M

R

Y

Y

N

N

L

G

D
x
C

P

E

A

R

V

V

R
x
D

F

F

W

L

A

S

T

T

P

P

I

V

H

P

G

G

C

L

L

L

T

R

G

R

S

Y

V

V

V

N

H

H

P

P

A

A

A

V

F

W

T

C

Y

H

R

K

L

I

P

-

D

G

N

N

V

M

S

S

F

Y

A

E

E

N

G

G

A

A

I

M

V

L

E

E

P
|
P

L

L

S

S

I
x
V

G
x
A

L

L

Q

A

A

G

A

L

T

Q

K

R

A

A

R

G

M

V

K

R

P

L

G

G

D

D

T

P

A

V

V

L

V

I

I

C

G
|
G

A

A

G
|
G

T
x
P

I
|
I

G

G

A

L

M

I

T

T

A

M

L

L

A

C

A

A

L

K

A

A

G

A

G

G

A

A

A

C

R

P

V

L

I

V

L

I

A
x
T

D
|
D

V
x
I

V

D

A

E

E

G

K
x
R

L

L

A

-

H

K

F

F

A

A

D

K

N

E

-

I

-

C

P

P

A

E

V

V

I

V

T

T

V

H

D

K

V
|
V

T

E

R

R

E

L

T

S

L

A

T

E

D

E

V

S

V

A

R

K

Q

K

A

I

T

V

D

E

G

S

W

F

G

G

A

G

-

I

-

E

-

P

D

A

V

V

V

A

F

L

E

E

A
x
C

S
x
T

G

G

H
x
V

A

E

G

S

V
x
S

Y

I

Q

A

T

A

L

A

L

I

D

W

L

A

V

V

C

K

P

F

G

G

C

K

A

V

V

F

L

V

V
x
I

G
|
G

M
x
V

P

G

P

K

A

N

P

E

V

I

A

Q

L

I

D

P

V

F

V

M

A

R

M

A

Q

S

T

V

K

R

E

E

V

V

R

D

L

L

E

Q

S

F

V
x
Q

F
x
Y

R
|
R

Y

Y

A

C

N

N

I

T

F

W

P

P

R

R

A

A

L

I

A

R

L

L

I

V

S

E

S

N

G

G

M

L

I

V

D

D

V

L

K

T

P

R

F

L

I

V

S

T

R

H

K

R

F

F

P

P

F

L

S

E

Q

D

S

A

I

L

R

K

A

A

F

F

E

E

E

T

A

A

A

S

S

D

G

P

R

K

P

T

Q

G

D

A

V

I

K
|
K

I

V

Q

Q

I

I

E

Q

active site: C49 (= C38), G50 (= G39), S51 (= S40), H54 (= H43), H74 (= H63), E75 (= E64), C104 (≠ R93), C107 (≠ S96), C110 (≠ T99), C118 (≠ D107), D122 (≠ R111), P160 (= P150), A164 (≠ G154), K352 (= K338)
binding nicotinamide-adenine-dinucleotide: C49 (= C38), G50 (= G39), S51 (= S40), V163 (≠ I153), G183 (= G173), G185 (= G175), P186 (≠ T176), I187 (= I177), T206 (≠ A196), D207 (= D197), I208 (≠ V198), R212 (≠ K202), V228 (= V217), C255 (≠ A241), T256 (≠ S242), V258 (≠ H244), S261 (≠ V247), I278 (≠ V264), G279 (= G265), V280 (≠ M266), Q302 (≠ V288), Y303 (≠ F289), R304 (= R290)
binding zinc ion: C49 (= C38), H74 (= H63), C104 (≠ R93), C107 (≠ S96), C110 (≠ T99), C118 (≠ D107)

7y9pA Xylitol dehydrogenase s96c/s99c/y102c mutant(thermostabilized form) from pichia stipitis (see paper)
35% identity, 96% coverage: 3:333/345 of query aligns to 5:347/357 of 7y9pA

query
sites
7y9pA

A

S

L

L

V

V

L

L

E

N

A

K

T

I

R

D

E

D

L

I

K

S

L

F

R

E

E

T

I

Y

D

D

L

A

P

P

Q

E

Q

I

M

S

G

E

A

P

Q

T

D

D

V

V

R

L

I

V

R

Q

I

V

H

K

T

K

V

T

G

G

I

I

C
|
C

G

G

S

S

D

D

L

I

H

H

Y

F

Y

Y

T

A

H

H

G

G

S

R

I

I

G

G

P

N

F

F

K

V

V

L

E

T

A

K

P

P

M

M

V

V

L

L

G

G

H
|
H

E
|
E

A

S

S

A

G

G

T

T

V

V

I

V

E

Q

V

V

G

G

S

K

A

G

V

V

S

T

H

S

L

L

K

K

V

V

G

G

D

D

R

N

V

V

C

A

M

I

E

E

P

P

G

G

I

I

P

P

R
x
C

L

R

D

F

S
x
C

P

D

A

E

T
x
C

L

K

R

S

G

G

M

H

Y

Y

N

N

L

L

D
x
C

P

P

A

H

V

M

R

A

F

F

W

A

A

A

T

T

P

P

P

N

I

E

-

P

-

N

-

P

H

P

G

G

C

T

L

L

T

C

G

K

S

Y

V

F

V

K

H

S

P

P

A

E

A

D

F

F

T

L

Y

V

R

K

L

L

P

P

D

D

N

H

V

V

S

S

F

L

A

E

E

L

G

G

A

A

I

L

V

V

E

E

P

P

L

L

S

S

I

V

G

G

L

V

Q

H

A

A

A

S

T

K

K

L

A

G

R

S

M

V

K

A

P

F

G

G

D

D

T

Y

A

V

V

A

V

V

I

F

G

G

A

A

G

G

T

P

I

V

G

G

A

L

M

L

T

A

A

A

L

A

A

V

A

A

L

K

A

T

G

F

G

G

A

A

A

K

R

G

V

V

I

I

L

V

A

V

D

D

V

I

V

F

A

D

E

N

K

K

L

L

A

K

H

M

F

A

A

K

D

D

N

I

P

G

A

A

V

A

I

T

T

H

V

T

D

F

V

N

T

S

R

K

E

T

T

G

L

G

T

S

D

E

V

E

V

L

R

I

Q

K

A

A

T

F

D

G

G

G

W

N

G

V

A

P

D

N

V

V

F

L

E

E

A

C

S

T

G

G

H

A

A

E

G

P

V

C

Y

I

Q

K

T

L

L

G

L

V

D

D

L

A

V

I

C

A

P

P

G

G

C

R

A

F

V

V

L

Q

V

V

G

G

M

N

P

A

A

G

P

P

V

V

A

S

L

F

D

P

V

I

V

T

A

V

M

F

Q

A

T

M

K

K

E

E

V

L

R

T

L

L

E

F

S

G

V

S

F

F

R

R

Y

Y

A

G

-

F

N

N

I

D

F

Y

P

K

R

T

A

A

L

V

A

G

L

I

I

F

S

D

S

T

G

N

M

Y

-

Q

-

N

-

G

-

R

-

E

-

N

-

A

-

P

I

I

D

D

V

F

K

E

P

Q

F

L

I

I

S

T

R

H

K

R

F

Y

P

K

F

F

S

K

Q

D

S

A

I

I

R

E

A

A

F

Y

E

D

E

L

A

V

A

R

S

A

G

G

R

K

binding zinc ion: C40 (= C38), H65 (= H63), E66 (= E64), C95 (≠ R93), C98 (≠ S96), C101 (≠ T99), C109 (≠ D107)

B6HI95 L-arabinitol 4-dehydrogenase; LAD; EC 1.1.1.12 from Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) (Penicillium chrysogenum) (see paper)
33% identity, 95% coverage: 15:341/345 of query aligns to 31:369/385 of B6HI95

query
sites
B6HI95

R

E

E

D

I

I

D

N

L

A

P

G

Q

K

Q

G

M

L

G

K

A

A

Q

G

D

E

V

V

R

T

I

I

R

E

I

V

H

R

T

S

V

T

G

G

I

I

C

C

G

G

S

S

D

D

L

V

H

H

Y

F

Y

W

T

H

H

A

G

G

S

C

I

I

G

G

P

P

F

M

K

I

V

V

E

T

A

G

P

D

M

H

V

V

L

L

G

G

H

H

E

E

A

S

S

A

G

G

T

Q

V

V

I

L

E

A

V

V

G

A

S

P

A

D

V

V

S

T

H

H

L

L

K

K

V

V

G

G

D

D

R

R

V

V

C

A

M

V

E

E

P

P

G

N

I

V

P

I

R

C

L

N

D

A

S

C

P

E

A

P

T

C

L

L

R

T

G

G

M

R

Y

Y

N

N

L

G

D

C

P

V

A

N

V

V

R

A

F

F

W

L

A

S

T

T

P

P

I

V

H

D

G

G

C

L

L

L

T

R

G

R

S

Y

V

V

V

N

H

H

P

P

A

A

A

V

F

W

T

C

Y

H

R

K

L

I

P

G

D

D

N

-

V

M

S

S

F

Y

A

E

E

D

G

G

A

A

I

M

V

L

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DI 212:213 (≠ DV 197:198) mutation to SR: Alters cofactor specificity from NAD to NADP; when associated with T-358.
S358 (≠ A330) mutation to T: Alters cofactor specificity from NAD to NADP; when associated with 212-SR-213.

Q96V44 L-arabinitol 4-dehydrogenase; LAD; EC 1.1.1.12 from Hypocrea jecorina (Trichoderma reesei) (see paper)
34% identity, 92% coverage: 27:342/345 of query aligns to 55:374/377 of Q96V44

query
sites
Q96V44

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DI 224:225 (≠ DV 197:198) mutation to SR: Alters cofactor specificity from NAD to NADP; when associated with T-362.
A362 (= A330) mutation to T: Alters cofactor specificity from NAD to NADP; when associated with 224-SR-225.

A2QAC0 L-arabinitol 4-dehydrogenase; LAD; EC 1.1.1.12 from Aspergillus niger (strain ATCC MYA-4892 / CBS 513.88 / FGSC A1513) (see 2 papers)
33% identity, 95% coverage: 14:341/345 of query aligns to 31:370/386 of A2QAC0

query
sites
A2QAC0

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