SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS19095 FitnessBrowser__HerbieS:HSERO_RS19095 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5odqB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with bromoethanesulfonate. (see paper)
25% identity, 58% coverage: 171:410/414 of query aligns to 5:268/291 of 5odqB

query
sites
5odqB

Y

F

L

F

E

L

G
|
G

C
|
C

V

I

Q

M

P

P

S

N

M

R

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Y

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A

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D

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M

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C

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G

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A

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x
G

G

G

T

G

Y

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S
x
R

I

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Q

E

P

L

E

E

L

L

S

S

Y

L

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D

L

M

R

T

D

N

N

E

K

K

I

I

S

E

K

N

L

M

Q

I

A

K

T

A

D

G

P

A

D

D

M

C

I

T

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V

T

-

G

-

N

N

I

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G
x
C

C

P

V
x
F

T
x
C

H

H

L
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L

Q

Q

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F

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D

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R

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I

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E

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I

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K

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E

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K

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F

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S

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G

E

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Y

D

N

T

F

P

P

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V

R

L

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H

W

L

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S

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Q

L

L

D

G

A

L

A

A

L

M

binding Non-cubane [4Fe-4S]-cluster: G8 (= G174), C9 (= C175), C41 (= C207), C42 (= C208), C78 (≠ A251), G80 (= G253), C81 (= C254), G152 (≠ A309), C153 (≠ Y310), H154 (= H311), C193 (= C346), C194 (= C347), C231 (≠ G386), F233 (≠ V388), C234 (≠ T389)
binding 2-bromanylethanesulfonic acid: G46 (≠ I211), G80 (= G253), H154 (= H311), G197 (≠ A350), R201 (≠ S354), F233 (≠ V388), L236 (= L391)

5odhH Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
25% identity, 58% coverage: 171:410/414 of query aligns to 5:268/291 of 5odhH

query
sites
5odhH

Y

F

L

F

E

L

G
|
G

C
|
C

V

I

Q

M

P

P

S

N

M

R

S

Y

P

A

N

G

I

V

N

E

S

A

A

A

T

T

A

R

R

T

V

V

L

M

D

E

A

K

L

L

G

G

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V

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E

L

L

F

V

A

D

P

M

P

T

K

G

A

A

G

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C
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C

G

P

A
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-
x
P

I
x
G

R

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Y

F

H

G

M

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N

F

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D

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Q

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K

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W

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R

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A

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W

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C

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A

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M

G

G

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A

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D

I

I

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x
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C
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C

G

Y

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G

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S

V

L

K

F

E

E

Y

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G

A

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Q

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A

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C

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x
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G
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G

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G

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x
R

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E

P

L

E

E

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Y

L

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L

M

R

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D

N

N

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M

Q

I

A

K

T

A

D

G

P

A

D

D

M

C

I

T

V

V

T

-

G

-

N

N

I

V

G
x
C

C

P

V
x
F

T
x
C

H

H

L

L

Q

Q

-

F

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D

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x
R

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G

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Q

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I

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E

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I

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K

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E

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K

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F

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N

T

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P

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V

R

L

H

H

W

L

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S

E

Q

L

L

D

G

A

L

A

A

L

M

binding Non-cubane [4Fe-4S]-cluster: G8 (= G174), C9 (= C175), C41 (= C207), C42 (= C208), C78 (≠ A251), G80 (= G253), C81 (= C254), C153 (≠ Y310), H154 (= H311), C193 (= C346), C194 (= C347), C231 (≠ G386), F233 (≠ V388), C234 (≠ T389)
binding 1-thioethanesulfonic acid: A44 (= A210), P45 (vs. gap), G46 (≠ I211), G80 (= G253), H154 (= H311), G197 (≠ A350), G198 (= G351)
binding Coenzyme B: R201 (≠ S354), F233 (≠ V388), R240 (vs. gap)

5odhB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
25% identity, 58% coverage: 171:410/414 of query aligns to 5:268/291 of 5odhB

query
sites
5odhB

Y

F

L

F

E

L

G
|
G

C
|
C

V
x
I

Q

M

P

P

S

N

M

R

S

Y

P

A

N

G

I

V

N

E

S

A

A

A

T

T

A

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R

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V

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M

D

E

A

K

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L

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G

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M

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G

A

A

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C
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C

G

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A

A

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x
P

I
x
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Y

F

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G

M

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N

F

D

D

Q

Q

E

K

G

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G

W

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A

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A

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R

N

N

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A

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C

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G

G

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R

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A

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D

I

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M

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x
C

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G

C
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C

G

Y

S

G

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V

L

K

F

E

E

Y

A

G

A

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H

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Q

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H

H

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H
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A

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C

G

G

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A

A

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G

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L

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E

P

L

E

E

L

L

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S

Y

L

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D

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M

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D

N

N

E

K

K

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I

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M

Q

I

A

K

T

A

D

G

P

A

D

D

M

C

I

T

V

V

T

-

G

-

N

N

I

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G
x
C

C

P

V
x
F

T
x
C

H

H

L

L

Q

Q

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F

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G

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Q

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I

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I

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K

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K

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K

G

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D

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T

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P

V

V

R

L

H

H

W

L

I

S

E

Q

L

L

D

G

A

L

A

A

L

M

binding Non-cubane [4Fe-4S]-cluster: G8 (= G174), C9 (= C175), I10 (≠ V176), C41 (= C207), C42 (= C208), C78 (≠ A251), G80 (= G253), C81 (= C254), G152 (≠ A309), C153 (≠ Y310), H154 (= H311), C193 (= C346), C194 (= C347), C231 (≠ G386), F233 (≠ V388), C234 (≠ T389)
binding 1-thioethanesulfonic acid: P45 (vs. gap), G46 (≠ I211), G80 (= G253), F233 (≠ V388)

5odcB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
25% identity, 58% coverage: 171:410/414 of query aligns to 5:268/291 of 5odcB

query
sites
5odcB

Y

F

L

F

E

L

G
|
G

C
|
C

V
x
I

Q

M

P

P

S

N

M

R

S

Y

P

A

N

G

I

V

N

E

S

A

A

A

T

T

A

R

R

T

V

V

L

M

D

E

A

K

L

L

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G

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V

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E

L

L

F

V

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M

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G

A

A

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C

G

P

A

A

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P

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Y

F

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N

F

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D

Q

Q

E

K

G

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G

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A

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A

R

R

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N

I

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D

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A

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W

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C

I

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M

G

G

I

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R

-

A

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D

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T

D

I

I

V

V

M

T

N

V

A
x
C

S
x
N

G
|
G

C
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C

G

Y

S

G

T

S

V

L

K

F

E

E

Y

A

G

A

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H

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Q

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H

H

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D

P

N

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Y

E

A

A

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F

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E

E

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I

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P

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F

I

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A

E

E

A

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L

K

N

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N

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x
G

Y
x
C

H

H

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C
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C

G

G

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A

A
x
G

G

G

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G

Y

V

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I

A

L

R

Q

E

P

L

E

E

L

L

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S

Y

L

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D

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D

N

N

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K

K

I

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A

K

T

A

D

G

P

A

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M

C

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V

T

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N

N

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x
C

C

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x
F

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x
C

H

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L

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Q

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F

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I

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L

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H

W

L

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S

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Q

L

L

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G

A

L

A

A

L

M

binding Non-cubane [4Fe-4S]-cluster: G8 (= G174), C9 (= C175), I10 (≠ V176), C41 (= C207), C42 (= C208), C78 (≠ A251), N79 (≠ S252), G80 (= G253), C81 (= C254), G152 (≠ A309), C153 (≠ Y310), C193 (= C346), C194 (= C347), G197 (≠ A350), C231 (≠ G386), F233 (≠ V388), C234 (≠ T389)

6myqB Avian mitochondrial complex ii with ferulenol bound (see paper)
28% identity, 23% coverage: 8:102/414 of query aligns to 134:234/239 of 6myqB

query
sites
6myqB

F

Y

I

L

R

Q

N

S

T

I

Q

E

A

D

G

R

Q

Q

E

K

A

L

D

D

S

G

I

-

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G

A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P

P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

-
x
A

-

V

-

L

-

M

-

Q

-

A

-

Y

R

R

G

W

R

M

I

I

Y

D

L

S

I

R

K

D

Q

D

V

Y

L

T

E

E

G

E

K

R

P

L

A

A

T

Q

A

L

A

Q

T

D

Q

P

S

F

H

S

L

L

D

Y

R

R

C
|
C

L

H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

R

T

T

C
|
C

P
|
P

S

K

G

G

V

L

Q

N

Y

P

G

G

R

K

L

A

V

I

D

A

I

E

G

I

R

K

K

K

V

M

binding 4-oxidanyl-3-[(2~{E},6~{E})-3,7,11-trimethyldodeca-2,6,10-trienyl]chromen-2-one: W165 (≠ L40), I210 (≠ C78)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (vs. gap), C217 (= C85), P218 (= P86)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

1yq3B Avian respiratory complex ii with oxaloacetate and ubiquinone (see paper)
28% identity, 23% coverage: 8:102/414 of query aligns to 137:237/242 of 1yq3B

query
sites
1yq3B

F

Y

I

L

R

Q

N

S

T

I

Q

E

A

D

G

R

Q

Q

E

K

A

L

D

D

S

G

I

-

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

-
x
A

-

V

-

L

-

M

-

Q

-

A

-

Y

R

R

G

W

R

M

I

I

Y

D

L

S

I

R

K

D

Q

D

V

Y

L

T

E

E

G

E

K

R

P

L

A

A

T

Q

A

L

A

Q

T

D

Q

P

S

F

H

S

L

L

D

Y

R

R

C
|
C

L

H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

R

T

T

C
|
C

P
|
P

S

K

G

G

V

L

Q

N

Y

P

G

G

R

K

L

A

V

I

D

A

I

E

G

I

R

K

K

K

V

M

binding fe3-s4 cluster: C163 (= C35), Y173 (≠ L45), P176 (= P48), C210 (= C75), T212 (= T77), I213 (≠ C78), M214 (≠ R79), N215 (= N80), C216 (= C81)
binding iron/sulfur cluster: C153 (= C25), I154 (≠ V26), L155 (≠ H27), C156 (= C28), A157 (≠ G29), C159 (= C31), A177 (vs. gap), C220 (= C85), P221 (= P86)
binding Coenzyme Q10, (2Z,6E,10Z,14E,18E,22E,26Z)-isomer: P164 (= P36), W168 (≠ L40), I213 (≠ C78)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 60, 61, 63, 65, 66, 67, 68, 80

6mysB Avian mitochondrial complex ii with atpenin a5 bound, sidechain outside (see paper)
28% identity, 23% coverage: 8:102/414 of query aligns to 135:235/240 of 6mysB

query
sites
6mysB

F

Y

I

L

R

Q

N

S

T

I

Q

E

A

D

G

R

Q

Q

E

K

A

L

D

D

S

G

I

-

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

-
x
A

-

V

-

L

-

M

-

Q

-

A

-

Y

R

R

G

W

R

M

I

I

Y

D

L

S

I

R

K

D

Q

D

V

Y

L

T

E

E

G

E

K

R

P

L

A

A

T

Q

A

L

A

Q

T

D

Q

P

S

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

R

T

T

C
|
C

P

P

S

K

G

G

V

L

Q

N

Y

P

G

G

R

K

L

A

V

I

D

A

I

E

G

I

R

K

K

K

V

M

binding 3-[(2s,4s,5r)-5,6-dichloro-2,4-dimethyl-1-oxohexyl]-4-hydroxy-5,6-dimethoxy-2(1h)-pyridinone: P162 (= P36), W166 (≠ L40), H209 (≠ L76), I211 (≠ C78)
binding fe3-s4 cluster: C161 (= C35), Y171 (≠ L45), P174 (= P48), C208 (= C75), T210 (= T77), I211 (≠ C78), M212 (≠ R79), N213 (= N80), C214 (= C81)
binding iron/sulfur cluster: C151 (= C25), I152 (≠ V26), L153 (≠ H27), C154 (= C28), A155 (≠ G29), C157 (= C31), A175 (vs. gap), C218 (= C85)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 59, 61, 63, 64, 65, 66, 78

6myrB Avian mitochondrial complex ii with thiapronil bound (see paper)
28% identity, 23% coverage: 8:102/414 of query aligns to 135:235/240 of 6myrB

query
sites
6myrB

F

Y

I

L

R

Q

N

S

T

I

Q

E

A

D

G

R

Q

Q

E

K

A

L

D

D

S

G

I

-

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

-
x
A

-

V

-

L

-

M

-

Q

-

A

-

Y

R

R

G

W

R

M

I

I

Y

D

L

S

I

R

K

D

Q

D

V

Y

L

T

E

E

G

E

K

R

P

L

A

A

T

Q

A

L

A

Q

T

D

Q

P

S

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

R

T

T

C
|
C

P

P

S

K

G

G

V

L

Q

N

Y

P

G

G

R

K

L

A

V

I

D

A

I

E

G

I

R

K

K

K

V

M

binding fe3-s4 cluster: C161 (= C35), Y171 (≠ L45), P174 (= P48), C208 (= C75), H209 (≠ L76), T210 (= T77), I211 (≠ C78), M212 (≠ R79), N213 (= N80), C214 (= C81)
binding thiapronil: P162 (= P36), W166 (≠ L40), H209 (≠ L76), I211 (≠ C78)
binding iron/sulfur cluster: C151 (= C25), I152 (≠ V26), L153 (≠ H27), C154 (= C28), A155 (≠ G29), C157 (= C31), A175 (vs. gap), C218 (= C85)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 58, 59, 63, 64, 65, 66, 78

6mypB Avian mitochondrial complex ii with ttfa (thenoyltrifluoroacetone) bound (see paper)
28% identity, 23% coverage: 8:102/414 of query aligns to 135:235/240 of 6mypB

query
sites
6mypB

F

Y

I

L

R

Q

N

S

T

I

Q

E

A

D

G

R

Q

Q

E

K

A

L

D

D

S

G

I

-

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L

Y

D

L

G

G

P

P

-
x
A

-

V

-

L

-

M

-

Q

-

A

-

Y

R

R

G

W

R

M

I

I

Y

D

L

S

I

R

K

D

Q

D

V

Y

L

T

E

E

G

E

K

R

P

L

A

A

T

Q

A

L

A

Q

T

D

Q

P

S

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C

I

R
x
M

N
|
N

C
|
C

E

T

S

R

T

T

C
|
C

P

P

S

K

G

G

V

L

Q

N

Y

P

G

G

R

K

L

A

V

I

D

A

I

E

G

I

R

K

K

K

V

M

binding fe3-s4 cluster: C161 (= C35), C208 (= C75), H209 (≠ L76), T210 (= T77), M212 (≠ R79), N213 (= N80), C214 (= C81)
binding iron/sulfur cluster: C151 (= C25), I152 (≠ V26), L153 (≠ H27), C154 (= C28), A155 (≠ G29), C157 (= C31), A175 (vs. gap), C218 (= C85)
binding 4,4,4-trifluoro-1-thien-2-ylbutane-1,3-dione: P162 (= P36), W166 (≠ L40), H209 (≠ L76)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 58, 59, 61, 63, 64, 65, 66, 78

6myoB Avian mitochondrial complex ii with flutolanyl bound (see paper)
28% identity, 23% coverage: 8:102/414 of query aligns to 135:235/240 of 6myoB

query
sites
6myoB

F

Y

I

L

R

Q

N

S

T

I

Q

E

A

D

G

R

Q

Q

E

K

A

L

D

D

S

G

I

-

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P

P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

-
x
A

-

V

-

L

-

M

-

Q

-

A

-

Y

R

R

G

W

R

M

I

I

Y

D

L

S

I

R

K

D

Q

D

V

Y

L

T

E

E

G

E

K

R

P

L

A

A

T

Q

A

L

A

Q

T

D

Q

P

S

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

R

T

T

C
|
C

P

P

S

K

G

G

V

L

Q

N

Y

P

G

G

R

K

L

A

V

I

D

A

I

E

G

I

R

K

K

K

V

M

binding fe3-s4 cluster: C161 (= C35), Y171 (≠ L45), P174 (= P48), C208 (= C75), T210 (= T77), I211 (≠ C78), M212 (≠ R79), N213 (= N80), C214 (= C81)
binding N-[3-(1-methylethoxy)phenyl]-2-(trifluoromethyl)benzamide: W166 (≠ L40), H209 (≠ L76), I211 (≠ C78)
binding iron/sulfur cluster: C151 (= C25), I152 (≠ V26), L153 (≠ H27), C154 (= C28), A155 (≠ G29), C157 (= C31), A175 (vs. gap), C218 (= C85)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 58, 59, 61, 63, 64, 65, 66, 78

2fbwB Avian respiratory complex ii with carboxin bound (see paper)
28% identity, 23% coverage: 8:102/414 of query aligns to 135:235/240 of 2fbwB

query
sites
2fbwB

F

Y

I

L

R

Q

N

S

T

I

Q

E

A

D

G

R

Q

Q

E

K

A

L

D

D

S

G

I

-

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G

A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

-
x
A

-

V

-

L

-

M

-

Q

-

A

-

Y

R

R

G

W

R

M

I

I

Y

D

L

S

I

R

K

D

Q

D

V

Y

L

T

E

E

G

E

K

R

P

L

A

A

T

Q

A

L

A

Q

T

D

Q

P

S

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

R

T

T

C
|
C

P
|
P

S

K

G

G

V

L

Q

N

Y

P

G

G

R

K

L

A

V

I

D

A

I

E

G

I

R

K

K

K

V

M

binding 2-methyl-n-phenyl-5,6-dihydro-1,4-oxathiine-3-carboxamide: P162 (= P36), W166 (≠ L40), H209 (≠ L76)
binding fe3-s4 cluster: C161 (= C35), Y171 (≠ L45), P174 (= P48), C208 (= C75), T210 (= T77), I211 (≠ C78), M212 (≠ R79), N213 (= N80), C214 (= C81)
binding iron/sulfur cluster: C151 (= C25), I152 (≠ V26), L153 (≠ H27), C154 (= C28), C157 (= C31), A175 (vs. gap), C218 (= C85), P219 (= P86)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 58, 59, 61, 63, 64, 65, 66, 78

3sfdB Crystal structure of porcine mitochondrial respiratory complex ii bound with oxaloacetate and pentachlorophenol (see paper)
27% identity, 23% coverage: 8:102/414 of query aligns to 134:234/239 of 3sfdB

query
sites
3sfdB

F

Y

I

L

R

Q

N

S

T

I

Q

E

A

E

G

R

Q

E

E

K

A

L

D

D

S

G

I

-

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P
|
P

T

S

Y

Y

Q

W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

-
x
A

-

V

-

L

-

M

-

Q

-

A

-

Y

R

R

G

W

R

M

I

I

Y

D

L

S

I

R

K

D

Q

D

V

F

L

T

E

E

G

E

K

R

P

L

A

A

T

K

A

L

A

Q

T

D

Q

P

S

F

H

S

L

L

D

Y

R

R

C
|
C

L

H

T
|
T

C
x
I

R
x
M

N
|
N

C
|
C

E

T

S

G

T

T

C
|
C

P
|
P

S
x
K

G

G

V

L

Q

N

Y

P

G

G

R

K

L

A

V

I

D

A

I

E

G

I

R

K

K

K

V

M

binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding pentachlorophenol: P161 (= P36), W165 (≠ L40), I210 (≠ C78)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (vs. gap), C217 (= C85), P218 (= P86), K219 (≠ S87)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 64, 65, 77

3aegB Crystal structure of porcine heart mitochondrial complex ii bound with n-biphenyl-3-yl-2-iodo-benzamide
27% identity, 23% coverage: 8:102/414 of query aligns to 134:234/239 of 3aegB

query
sites
3aegB

F

Y

I

L

R

Q

N

S

T

I

Q

E

A

E

G

R

Q

E

E

K

A

L

D

D

S

G

I

-

L

L

R

Y

S

E

C
|
C

V
x
I

H
x
L

C
|
C

G
x
A

F

C

C
|
C

T

S

A

T

T

S

C
|
C

P

P

T
x
S

Y

Y

Q
x
W

L
x
W

L

N

G

G

D

D

E

K

L
x
Y

D

L

G

G

P
|
P

-
x
A

-

V

-

L

-

M

-

Q

-

A

-

Y

R

R

G

W

R

M

I

I

Y

D

L

S

I

R

K

D

Q

D

V

F

L

T

E

E

G

E

K

R

P

L

A

A

T

K

A

L

A

Q

T

D

Q

P

S

F

H

S

L

L

D

Y

R

R

C
|
C

L
x
H

T

T

C
x
I

R
x
M

N

N

C
|
C

E

T

S

G

T

T

C
|
C

P

P

S

K

G

G

V

L

Q

N

Y

P

G

G

R

K

L

A

V
x
I

D

A

I

E

G

I

R

K

K

K

V

M

binding N-biphenyl-3-yl-2-iodobenzamide: S162 (≠ T37), W164 (≠ Q39), W165 (≠ L40), H208 (≠ L76)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), I210 (≠ C78), M211 (≠ R79), C213 (= C81), I227 (≠ V95)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (vs. gap), C217 (= C85)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 75, 77

3aeeB Crystal structure of porcine heart mitochondrial complex ii bound with atpenin a5
27% identity, 23% coverage: 8:102/414 of query aligns to 134:234/239 of 3aeeB

query
sites
3aeeB

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binding 3-[(2s,4s,5r)-5,6-dichloro-2,4-dimethyl-1-oxohexyl]-4-hydroxy-5,6-dimethoxy-2(1h)-pyridinone: W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (vs. gap), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3aedB Crystal structure of porcine heart mitochondrial complex ii bound with 2-iodo-n-phenyl-benzamide
27% identity, 23% coverage: 8:102/414 of query aligns to 134:234/239 of 3aedB

query
sites
3aedB

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binding 2-iodo-N-phenylbenzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), C217 (= C85), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3aecB Crystal structure of porcine heart mitochondrial complex ii bound with 2-iodo-n-(1-methylethyl)-benzamid
27% identity, 23% coverage: 8:102/414 of query aligns to 134:234/239 of 3aecB

query
sites
3aecB

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binding 2-iodo-N-(1-methylethyl)benzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L76)
binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (vs. gap), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 62, 63, 65, 75, 77

3aebB Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-phenoxy-phenyl)-2-trifluoromethyl-benzamide
27% identity, 23% coverage: 8:102/414 of query aligns to 134:234/239 of 3aebB

query
sites
3aebB

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binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding N-(3-phenoxyphenyl)-2-(trifluoromethyl)benzamide: W165 (≠ L40), H208 (≠ L76)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (vs. gap), C217 (= C85), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 62, 63, 65, 77

3aeaB Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-dimethylaminomethyl-phenyl)-2-trifluoromethyl-benzamide (see paper)
27% identity, 23% coverage: 8:102/414 of query aligns to 134:234/239 of 3aeaB

query
sites
3aeaB

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binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding N-{3-[(dimethylamino)methyl]phenyl}-2-(trifluoromethyl)benzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L76), I210 (≠ C78)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (vs. gap), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3ae9B Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-pentafluorophenyloxy-phenyl)-2-trifluoromethyl-benzamide (see paper)
27% identity, 23% coverage: 8:102/414 of query aligns to 134:234/239 of 3ae9B

query
sites
3ae9B

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binding fe3-s4 cluster: C160 (= C35), Y170 (≠ L45), P173 (= P48), C207 (= C75), H208 (≠ L76), T209 (= T77), I210 (≠ C78), M211 (≠ R79), N212 (= N80), C213 (= C81)
binding N-[3-(pentafluorophenoxy)phenyl]-2-(trifluoromethyl)benzamide: P161 (= P36), W165 (≠ L40), H208 (≠ L76)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), A154 (≠ G29), C156 (= C31), A174 (vs. gap), C217 (= C85), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 60, 62, 63, 65, 77

3ae8B Crystal structure of porcine heart mitochondrial complex ii bound with n-(3-isopropoxy-phenyl)-2-trifluoromethylbenzamide
27% identity, 23% coverage: 8:102/414 of query aligns to 134:234/239 of 3ae8B

query
sites
3ae8B

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binding fe3-s4 cluster: C160 (= C35), P173 (= P48), C207 (= C75), T209 (= T77), I210 (≠ C78), M211 (≠ R79), C213 (= C81)
binding N-[3-(1-methylethoxy)phenyl]-2-(trifluoromethyl)benzamide: S162 (≠ T37), W165 (≠ L40), H208 (≠ L76)
binding iron/sulfur cluster: C150 (= C25), I151 (≠ V26), L152 (≠ H27), C153 (= C28), C156 (= C31), A174 (vs. gap), C217 (= C85), P218 (= P86), L221 (≠ V89)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 57, 58, 62, 63, 65, 75, 77

Query Sequence

>HSERO_RS19095 FitnessBrowser__HerbieS:HSERO_RS19095
MQTNLADFIRNTQAGQEADSILRSCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLE
GKPATAATQSHLDRCLTCRNCESTCPSGVQYGRLVDIGRKVVEEQVPRPLSQRVMRTALK
ELVPRKWIFRPAMKAGQMLRPLLPKLLQNKVPLPQEAGAWPTRTHARQMLYLEGCVQPSM
SPNINSATARVLDALGVQLFAPPKAGCCGAIRYHMNDQEGGLEDMRRNIDAWWPYIEGRD
GIRADTIVMNASGCGSTVKEYGHLLQHDPVYAEKARRISHMTRDISEILPEFEEALKHKL
KDFNGKRVAYHPPCTLQHGQKIRGKVEGILRAAGVDVQLCADSHLCCGSAGTYSILQPEL
SYRLRDNKISKLQATDPDMIVTGNIGCVTHLQSGTDTPVRHWIELLDAALQAPS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory