SitesBLAST
Comparing HSERO_RS19510 FitnessBrowser__HerbieS:HSERO_RS19510 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P04968 L-threonine dehydratase biosynthetic IlvA; Threonine deaminase; EC 4.3.1.19 from Escherichia coli (strain K12) (see paper)
51% identity, 99% coverage: 4:507/508 of query aligns to 15:512/514 of P04968
- K62 (= K51) modified: N6-(pyridoxal phosphate)lysine
- N89 (= N78) binding
- GGGGL 188:192 (= GGGGL 178:182) binding
- S315 (≠ C310) binding
1tdjA Threonine deaminase (biosynthetic) from e. Coli (see paper)
50% identity, 99% coverage: 4:507/508 of query aligns to 11:492/494 of 1tdjA
- active site: K58 (= K51), A83 (= A76), E209 (≠ Q203), S213 (= S207), C215 (≠ A209), G237 (= G231), L310 (≠ A309), S311 (≠ C310)
- binding pyridoxal-5'-phosphate: F57 (= F50), K58 (= K51), N85 (= N78), G184 (= G178), G185 (= G179), G186 (= G180), G187 (= G181), G237 (= G231), E282 (= E276), S311 (≠ C310), G312 (= G311)
2gn2A Crystal structure of tetrameric biodegradative threonine deaminase (tdcb) from salmonella typhimurium in complex with cmp at 2.5a resolution (hexagonal form) (see paper)
36% identity, 63% coverage: 7:327/508 of query aligns to 16:326/326 of 2gn2A
- active site: K56 (= K51), A81 (= A76), Q207 (= Q203), V211 (≠ S207), G213 (≠ A209), G235 (= G231), I308 (≠ A309), S309 (≠ C310)
- binding cytidine-5'-monophosphate: R51 (≠ S46), T52 (≠ V47), G53 (≠ F48), A114 (= A109), D117 (≠ A112), Y118 (≠ R113), N312 (= N313)
Q7XSN8 Serine racemase; D-serine dehydratase; D-serine ammonia-lyase; L-serine dehydratase; L-serine ammonia-lyase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Oryza sativa subsp. japonica (Rice) (see paper)
35% identity, 54% coverage: 12:286/508 of query aligns to 29:301/339 of Q7XSN8
- E219 (≠ Q203) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-225.
- D225 (≠ A209) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-219.
1wtcA Crystal structure of s.Pombe serine racemase complex with amppcp (see paper)
33% identity, 61% coverage: 11:318/508 of query aligns to 12:311/318 of 1wtcA
- active site: K52 (= K51), S77 (≠ A76), E203 (≠ Q203), G207 (≠ S207), D209 (≠ A209), G231 (= G231), I302 (≠ A309), S303 (≠ C310)
- binding phosphomethylphosphonic acid adenylate ester: N20 (≠ Q19), K47 (≠ S46), M48 (≠ V47), A109 (≠ D108), A110 (= A109), Y114 (≠ R113)
- binding magnesium ion: E203 (≠ Q203), G207 (≠ S207), D209 (≠ A209)
- binding pyridoxal-5'-phosphate: F51 (= F50), K52 (= K51), N79 (= N78), G178 (= G178), G179 (= G179), G180 (= G180), G181 (= G181), G231 (= G231), E276 (= E276), T278 (≠ A278), S303 (≠ C310)
1v71A Crystal structure of s.Pombe serine racemase
33% identity, 61% coverage: 11:318/508 of query aligns to 12:311/318 of 1v71A
- active site: K52 (= K51), S77 (≠ A76), E203 (≠ Q203), G207 (≠ S207), D209 (≠ A209), G231 (= G231), I302 (≠ A309), S303 (≠ C310)
- binding magnesium ion: E203 (≠ Q203), G207 (≠ S207), D209 (≠ A209)
- binding pyridoxal-5'-phosphate: F51 (= F50), K52 (= K51), N79 (= N78), G178 (= G178), G179 (= G179), G180 (= G180), G181 (= G181), G231 (= G231), E276 (= E276), T278 (≠ A278), S303 (≠ C310), G304 (= G311)
O59791 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 4.3.1.17; EC 4.3.1.18; EC 5.1.1.18 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
33% identity, 61% coverage: 11:318/508 of query aligns to 17:316/323 of O59791
- S82 (≠ A76) mutation to A: Loss of racemase activity. Reduces D-serine dehydratase activity by 99%. Slightly reduced L-serine dehydratase activity.
2zr8A Crystal structure of modified serine racemase complexed with serine (see paper)
33% identity, 61% coverage: 11:318/508 of query aligns to 13:312/319 of 2zr8A
- active site: K53 (= K51), S78 (≠ A76), E204 (≠ Q203), G208 (≠ S207), D210 (≠ A209), G232 (= G231), I303 (≠ A309), S304 (≠ C310)
- binding magnesium ion: E204 (≠ Q203), G208 (≠ S207), D210 (≠ A209)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F50), K53 (= K51), S77 (= S75), S78 (≠ A76), N80 (= N78), H81 (= H79), P147 (= P145), G179 (= G178), G180 (= G179), G181 (= G180), G182 (= G181), G232 (= G231), E277 (= E276), T279 (≠ A278), S304 (≠ C310)
- binding serine: S78 (≠ A76), R129 (≠ A127), D231 (= D230), G232 (= G231), A233 (≠ T232), Q234 (≠ A233), T235 (≠ V234)
2zpuA Crystal structure of modified serine racemase from s.Pombe. (see paper)
33% identity, 61% coverage: 11:318/508 of query aligns to 13:312/319 of 2zpuA
- active site: K53 (= K51), S78 (≠ A76), E204 (≠ Q203), G208 (≠ S207), D210 (≠ A209), G232 (= G231), I303 (≠ A309), S304 (≠ C310)
- binding magnesium ion: E204 (≠ Q203), G208 (≠ S207), D210 (≠ A209)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F50), K53 (= K51), S77 (= S75), S78 (≠ A76), N80 (= N78), H81 (= H79), P147 (= P145), G179 (= G178), G180 (= G179), G181 (= G180), G182 (= G181), G232 (= G231), E277 (= E276), T279 (≠ A278), S304 (≠ C310)
A4F2N8 L-threo-3-hydroxyaspartate ammonia-lyase; L-threo-3-hydroxyaspartate dehydratase; L-THA DH; EC 4.3.1.16 from Pseudomonas sp. (see paper)
32% identity, 60% coverage: 13:318/508 of query aligns to 15:312/319 of A4F2N8
- K53 (= K51) mutation to A: Loss of enzymatic activity.
5cvcA Structure of maize serine racemase (see paper)
34% identity, 61% coverage: 12:319/508 of query aligns to 13:316/329 of 5cvcA
- active site: K52 (= K51), S77 (≠ A76), E203 (≠ Q203), A207 (≠ S207), D209 (≠ A209), G231 (= G231), V306 (≠ A309), S307 (≠ C310)
- binding magnesium ion: E203 (≠ Q203), A207 (≠ S207), D209 (≠ A209)
- binding pyridoxal-5'-phosphate: F51 (= F50), K52 (= K51), N79 (= N78), S178 (≠ G178), G179 (= G179), G180 (= G180), G181 (= G181), L232 (≠ T232), E275 (= E276), S307 (≠ C310), G308 (= G311)
7nbhAAA structure of human serine racemase in complex with DSiP fragment Z26781964, XChem fragment screen (see paper)
32% identity, 54% coverage: 13:285/508 of query aligns to 15:289/320 of 7nbhAAA
- active site: K53 (= K51), S81 (≠ A76), E207 (≠ Q203), A211 (≠ S207), D213 (≠ A209), G236 (= G231)
- binding calcium ion: E207 (≠ Q203), A211 (≠ S207), D213 (≠ A209)
- binding N-[(1H-benzimidazol-2-yl)methyl]furan-2-carboxamide: S81 (≠ A76), G85 (≠ A80), Q86 (= Q81), K111 (= K106), I115 (≠ V110), Y118 (≠ R113), D235 (= D230), P281 (= P277)
Sites not aligning to the query:
7nbfAAA structure of human serine racemase in complex with DSiP fragment Z126932614, XChem fragment screen (see paper)
32% identity, 54% coverage: 13:285/508 of query aligns to 15:289/323 of 7nbfAAA
- active site: K53 (= K51), S81 (≠ A76), E207 (≠ Q203), A211 (≠ S207), D213 (≠ A209), G236 (= G231)
- binding calcium ion: E207 (≠ Q203), A211 (≠ S207), D213 (≠ A209)
- binding magnesium ion: N244 (≠ E240)
- binding pyridoxal-5'-phosphate: F52 (= F50), K53 (= K51), N83 (= N78), G182 (= G178), G183 (= G179), G184 (= G180), G185 (= G181), M186 (≠ L182), G236 (= G231), V237 (≠ T232), T282 (≠ A278)
- binding 2-[(methylsulfonyl)methyl]-1H-benzimidazole: H21 (≠ Q19), L22 (≠ E20), T23 (= T21), P24 (= P22), L26 (≠ E24), T27 (≠ L25), F46 (≠ M44)
Sites not aligning to the query:
7nbdAAA structure of human serine racemase in complex with DSiP fragment Z235449082, XChem fragment screen (see paper)
32% identity, 54% coverage: 13:285/508 of query aligns to 15:289/323 of 7nbdAAA
- active site: K53 (= K51), S81 (≠ A76), E207 (≠ Q203), A211 (≠ S207), D213 (≠ A209), G236 (= G231)
- binding calcium ion: E207 (≠ Q203), A211 (≠ S207), D213 (≠ A209)
- binding [4-(1H-benzimidazol-1-yl)phenyl]methanol: W272 (≠ F268), L278 (≠ I274)
- binding magnesium ion: N244 (≠ E240)
- binding pyridoxal-5'-phosphate: F52 (= F50), K53 (= K51), N83 (= N78), G182 (= G178), G183 (= G179), G184 (= G180), G185 (= G181), M186 (≠ L182), G236 (= G231), V237 (≠ T232), E280 (= E276), T282 (≠ A278)
Sites not aligning to the query:
7nbcCCC structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
32% identity, 54% coverage: 13:285/508 of query aligns to 15:289/323 of 7nbcCCC
- active site: K53 (= K51), S81 (≠ A76), E207 (≠ Q203), A211 (≠ S207), D213 (≠ A209), G236 (= G231)
- binding biphenyl-4-ylacetic acid: T78 (≠ C73), H79 (≠ A74), H84 (= H79), V148 (= V143), H149 (= H144), P150 (= P145)
- binding calcium ion: E207 (≠ Q203), A211 (≠ S207), D213 (≠ A209)
- binding pyridoxal-5'-phosphate: F52 (= F50), K53 (= K51), N83 (= N78), G182 (= G178), G183 (= G179), G184 (= G180), G185 (= G181), M186 (≠ L182), G236 (= G231), V237 (≠ T232), T282 (≠ A278)
Sites not aligning to the query:
7nbcAAA structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
32% identity, 54% coverage: 13:285/508 of query aligns to 15:289/323 of 7nbcAAA
- active site: K53 (= K51), S81 (≠ A76), E207 (≠ Q203), A211 (≠ S207), D213 (≠ A209), G236 (= G231)
- binding calcium ion: E207 (≠ Q203), A211 (≠ S207), D213 (≠ A209)
- binding magnesium ion: N244 (≠ E240)
- binding pyridoxal-5'-phosphate: F52 (= F50), K53 (= K51), N83 (= N78), G182 (= G178), G183 (= G179), G184 (= G180), G185 (= G181), M186 (≠ L182), G236 (= G231), V237 (≠ T232), T282 (≠ A278)
Sites not aligning to the query:
7nbgAAA structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
32% identity, 54% coverage: 13:285/508 of query aligns to 15:289/322 of 7nbgAAA
- active site: K53 (= K51), S81 (≠ A76), E207 (≠ Q203), A211 (≠ S207), D213 (≠ A209), G236 (= G231)
- binding calcium ion: E207 (≠ Q203), A211 (≠ S207), D213 (≠ A209)
- binding pyridoxal-5'-phosphate: F52 (= F50), K53 (= K51), N83 (= N78), G182 (= G178), G183 (= G179), G184 (= G180), G185 (= G181), M186 (≠ L182), G236 (= G231), V237 (≠ T232), T282 (≠ A278)
- binding ~{N}-[2-(2-methylphenyl)ethyl]ethanamide: S81 (≠ A76), G85 (≠ A80), Q86 (= Q81), I101 (= I96), K111 (= K106), I115 (≠ V110), Y118 (≠ R113)
Sites not aligning to the query:
6zspAAA serine racemase bound to atp and malonate. (see paper)
32% identity, 54% coverage: 13:285/508 of query aligns to 15:282/320 of 6zspAAA
- active site: K53 (= K51), S74 (≠ A76), E200 (≠ Q203), A204 (≠ S207), D206 (≠ A209), G229 (= G231)
- binding adenosine-5'-triphosphate: S28 (≠ A26), S29 (≠ N27), I30 (≠ T28), K48 (≠ S46), T49 (≠ V47), Q79 (= Q81), Y111 (≠ R113), E266 (≠ Q269), R267 (≠ D270), K269 (≠ R272)
- binding magnesium ion: E200 (≠ Q203), A204 (≠ S207), D206 (≠ A209)
- binding malonate ion: K53 (= K51), S73 (= S75), S74 (≠ A76), N76 (= N78), H77 (= H79), R125 (≠ A127), G229 (= G231), S232 (≠ V234)
Sites not aligning to the query:
1ve5A Crystal structure of t.Th. Hb8 threonine deaminase
34% identity, 60% coverage: 13:317/508 of query aligns to 12:307/308 of 1ve5A
- active site: K50 (= K51), S56 (≠ N57), S72 (≠ A76), E200 (≠ Q203), A204 (≠ S207), D206 (≠ A209), G229 (= G231), L299 (≠ A309), S300 (≠ C310)
- binding calcium ion: E200 (≠ Q203), A204 (≠ S207), D206 (≠ A209)
- binding pyridoxal-5'-phosphate: F49 (= F50), K50 (= K51), N74 (= N78), G175 (vs. gap), G176 (vs. gap), G177 (vs. gap), G178 (vs. gap), E274 (= E276), T276 (≠ A278), S300 (≠ C310), G301 (= G311)
3l6bA X-ray crystal structure of human serine racemase in complex with malonate a potent inhibitor (see paper)
31% identity, 54% coverage: 13:285/508 of query aligns to 16:285/322 of 3l6bA
- active site: K54 (= K51), S77 (≠ A76), E203 (≠ Q203), A207 (≠ S207), D209 (≠ A209), G232 (= G231), T278 (≠ A278)
- binding malonate ion: K54 (= K51), S76 (= S75), S77 (≠ A76), N79 (= N78), H80 (= H79), R128 (≠ A127), G232 (= G231)
- binding manganese (ii) ion: E203 (≠ Q203), A207 (≠ S207), D209 (≠ A209)
- binding pyridoxal-5'-phosphate: F53 (= F50), K54 (= K51), N79 (= N78), G178 (= G178), G179 (= G179), G180 (= G180), G181 (= G181), M182 (≠ L182), V233 (≠ T232), E276 (= E276), T278 (≠ A278)
Sites not aligning to the query:
Query Sequence
>HSERO_RS19510 FitnessBrowser__HerbieS:HSERO_RS19510
MSNDYLKKILTARVYDVAQETPLELANTLSQRIDNRIYFKREDMQSVFSFKLRGAYNKMA
HLTPAQLKRGVICASAGNHAQGVALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLF
GDSFTDAYEHALTLEKKQKLTFVHPFDDPYVIAGQGTVGMEILRQHPDPIHAIFVAIGGG
GLIAGVASYVKAVRPDIKIIGVQTTDSDAMARSLKAGRRVALPDVGLFSDGTAVKLVGEE
TFRIAKELVDEVIIVDTDAVCTAIKDIFQDTRSIVEPAGALAVAGAKAYVERAKASKKPI
KGESLITIACGANMNFDRLRFVAEMADAGEAREAVFAVTIPEERGSFRRFCETVGPRNVT
EFNYRISDAKAAHVFVGIQVSAADEAGKIARNFEKAGFGVLDLTHDELAKVHIRHLVGGK
SDLTGDELLYRFEFPERPGALMKFLSSMNPGWNISLFHYRNQGGDVGRILIGLQVPKKEM
KEFRAFLAQLGYRHWDESKNPLYKLFLG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory