SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS20310 FitnessBrowser__HerbieS:HSERO_RS20310 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

7bgnA Crystal structure of mthisn2-amp complex, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
44% identity, 60% coverage: 8:107/166 of query aligns to 6:104/204 of 7bgnA

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7bgnA

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binding adenosine monophosphate: W60 (= W62), K62 (= K64), G63 (= G65), T65 (≠ E67), S66 (= S68), T94 (≠ A97), H96 (= H99)
binding zinc ion: D78 (= D80), D80 (= D82), D82 (= D84), C95 (= C98), C102 (= C105)

Sites not aligning to the query:

binding adenosine monophosphate: 121, 128, 135, 136, 137, 180, 203, 204
binding zinc ion: 154, 174, 177

7bgmA Crystal structure of mthisn2, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
44% identity, 60% coverage: 8:107/166 of query aligns to 8:106/213 of 7bgmA

query
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7bgmA

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binding zinc ion: D80 (= D80), D82 (= D82), D84 (= D84), C97 (= C98), C104 (= C105)

Sites not aligning to the query:

binding zinc ion: 163, 183, 186

6j2lA Crystal structure of bi-functional enzyme (see paper)
44% identity, 58% coverage: 10:106/166 of query aligns to 8:104/200 of 6j2lA

query
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6j2lA

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binding zinc ion: D79 (= D80), D81 (= D82), D83 (= D84), C96 (= C98), C103 (= C105)

Sites not aligning to the query:

binding zinc ion: 152, 171, 174

6j2lB Crystal structure of bi-functional enzyme (see paper)
38% identity, 61% coverage: 10:111/166 of query aligns to 7:111/185 of 6j2lB

query
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6j2lB

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binding zinc ion: D78 (= D80), D80 (= D82), D82 (= D84)

Sites not aligning to the query:

binding magnesium ion: 137, 140, 156, 159

Query Sequence

>HSERO_RS20310 FitnessBrowser__HerbieS:HSERO_RS20310
MSISAKWLNKVKWDEVGLVPVIAQEVGSNDVLMFAWMNREALARTVEIGQAVYWSRSRKK
LWHKGEESGHFQKVHEIRLDCDEDVVLLKVEQVAGIACHTGRHSCFFQKFEGSADSGEWV
TVEPVLKDLQPGAGGGPAATAAEPEAKPKRVRKSIPKPVDSDKTPS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory