SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS20985 FitnessBrowser__HerbieS:HSERO_RS20985 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3mqhA Crystal structure of the 3-n-acetyl transferase wlbb from bordetella petrii in complex with coa and udp-3-amino-2-acetamido-2,3-dideoxy glucuronic acid (see paper)
64% identity, 96% coverage: 6:185/188 of query aligns to 6:188/191 of 3mqhA

query
sites
3mqhA

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binding coenzyme a: F76 (= F76), T84 (= T84), N85 (= N85), V86 (= V86), Y87 (≠ N87), P89 (= P89), G111 (= G111), A112 (= A112), A130 (= A130), N135 (≠ T135), V145 (= V145), P148 (= P148), R150 (= R150), Q161 (≠ R161)
binding (2S,3S,4R,5R,6R)-5-(acetylamino)-4-amino-6-{[(R)-{[(R)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-3-hydroxytetrahydro-2H-pyran-2-carboxylic acid: W24 (= W24), H28 (= H28), F46 (= F46), Q60 (= Q60), N61 (= N61), Y66 (= Y66), F76 (= F76), N85 (= N85), V86 (= V86), R95 (= R95), Y99 (= Y99)

3mqgC Crystal structure of the 3-n-acetyl transferase wlbb from bordetella petrii in complex with acetyl-coa (see paper)
64% identity, 96% coverage: 6:185/188 of query aligns to 7:189/192 of 3mqgC

query
sites
3mqgC

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binding acetyl coenzyme *a: Y67 (= Y66), F77 (= F76), P80 (= P79), T85 (= T84), N86 (= N85), V87 (= V86), Y88 (≠ N87), P90 (= P89), G112 (= G111), A113 (= A112), F128 (= F127), G130 (= G129), A131 (= A130), N136 (≠ T135), V146 (= V145), P149 (= P148), R151 (= R150), Q162 (≠ R161)
binding uridine-5'-monophosphate: H29 (= H28), F47 (= F46)
binding uridine-5'-diphosphate: W25 (= W24), S41 (= S40), N62 (= N61)

G3XD01 UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronate N-acetyltransferase; UDP-D-GlcNAc3NA N-acetyltransferase; UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronic acid 3-N-acetyltransferase; EC 2.3.1.201 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
62% identity, 96% coverage: 1:181/188 of query aligns to 1:182/191 of G3XD01

query
sites
G3XD01

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K136 (≠ R136) mutation to A: Reduction in catalytic activity.; mutation to R: No effect on catalytic activity.

4mzuF Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
43% identity, 80% coverage: 6:155/188 of query aligns to 3:144/294 of 4mzuF

query
sites
4mzuF

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binding coenzyme a: N81 (= N85), D82 (≠ V86), K83 (≠ N87), G100 (= G111), A101 (= A112), L106 (≠ V117), G118 (= G129), A119 (= A130), T124 (= T135), K125 (≠ R136), I134 (≠ V145), N136 (≠ T147), R139 (= R150)
binding thymine: W20 (= W24), Q21 (≠ H25), C38 (≠ G42)

Sites not aligning to the query:

binding thymidine-5'-diphosphate: 189, 203, 205, 270

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
43% identity, 80% coverage: 6:155/188 of query aligns to 3:143/290 of 4mzuB

query
sites
4mzuB

H

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T

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binding coenzyme a: F72 (= F76), T80 (= T84), N81 (= N85), D82 (≠ V86), K83 (≠ N87), P85 (= P89), G99 (= G111), A100 (= A112), G117 (= G129), A118 (= A130), T123 (= T135), K124 (≠ R136), I133 (≠ V145), R138 (= R150)
binding magnesium ion: G58 (≠ N62), N76 (≠ S80)
binding thymine: W20 (= W24), Q21 (≠ H25), C38 (≠ G42), A39 (≠ Q43)

Sites not aligning to the query:

binding coenzyme a: 199
binding thymidine-5'-diphosphate: 171, 176, 189, 191, 202, 203, 270

7s42A Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-acetamido-3,6-dideoxy-d- galactose (see paper)
49% identity, 71% coverage: 6:138/188 of query aligns to 5:135/145 of 7s42A

query
sites
7s42A

H

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binding [(2~{R},3~{R},4~{S},5~{R},6~{R})-4-acetamido-6-methyl-3,5-bis(oxidanyl)oxan-2-yl] [[(2~{R},3~{S},5~{R})-5-[5-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: F44 (= F46), E46 (≠ G48), W64 (≠ Y66), N83 (= N85), D84 (≠ V86), Y92 (≠ S94)
binding coenzyme a: C82 (≠ T84), D84 (≠ V86), K85 (≠ N87), Y86 (≠ N88), P87 (= P89), L114 (≠ V117), T132 (= T135), K133 (≠ R136)

7s41A Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-acetamido-3,6-dideoxy-d- glucose (see paper)
49% identity, 71% coverage: 6:138/188 of query aligns to 5:135/145 of 7s41A

query
sites
7s41A

H

H

E

K

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W

W

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W

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S

A

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G

G

E

E

R

N

C

C

S

N

F

I

G

C

Q

S

N

H

V

C

F
|
F

V

I

G
x
E

N

N

D

D

V

V

L

K

I

I

G

G

N

N

V

V

K

T

V

I

Q

K

N

C

N

G

V

V

S

Q

I

I

Y
x
W

D

D

A

G

V

I

T

E

L

I

E

E

D

D

V

V

F

F

C

I

G

G

P

P

S

N

M

V

V

T

F

F

T
x
C

N
|
N

V
x
D

N
x
K

N
x
Y

P
|
P

R

R

S

S

A

K

V

Q

S
x
Y

R

P

K

K

H

-

E

E

Y

F

R

S

R

K

T

T

L

I

V

I

R

K

G

G

A

A

S

S

I

I

G

G

A

A

N

N

A

A

T

T

I

I

V
x
L

C

P

G

G

H

I

E

T

I

I

G

G

E

E

Y

N

A

A

F

M

I

I

G

G

A

A

G

G

A

A

V

I

V

V

T
|
T

R
x
K

D

D

V

V

binding [(2~{R},3~{R},4~{S},5~{S},6~{R})-4-acetamido-6-methyl-3,5-bis(oxidanyl)oxan-2-yl] [[(2~{R},3~{S},5~{R})-5-[5-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate : F44 (= F46), E46 (≠ G48), W64 (≠ Y66), N83 (= N85), D84 (≠ V86), Y92 (≠ S94)
binding coenzyme a: C82 (≠ T84), D84 (≠ V86), K85 (≠ N87), Y86 (≠ N88), P87 (= P89), L114 (≠ V117), T132 (= T135), K133 (≠ R136)

7s3wA Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-amino-3,6-dideoxy-d- galactose (see paper)
49% identity, 71% coverage: 6:138/188 of query aligns to 5:135/145 of 7s3wA

query
sites
7s3wA

H

H

E

K

T

L

A

A

L

D

V

V

D

-

E

Q

G

S

A

K

Q

N

I

I

G

G

E

S

A

G

T

T

R

R

I

I

W

W

H

Q

W

F

A

C

H

V

I

V

C

L

S

P

G

S

A

A

R

I

I

I

G

G

E

E

R

N

C

C

S

N

F

I

G

C

Q

S

N

H

V

C

F
|
F

V

I

G
x
E

N

N

D

D

V

V

L

K

I

I

G

G

N

N

V

V

K

T

V

I

Q

K

N

C

N

G

V

V

S

Q

I

I

Y

W

D

D

A

G

V

I

T

E

L

I

E

E

D

D

V

V

F

F

C

I

G

G

P

P

S

N

M

V

V

T

F

F

T
x
C

N

N

V
x
D

N
x
K

N
x
Y

P
|
P

R

R

S

S

A

K

V

Q

S
x
Y

R

P

K

K

H

-

E

E

Y

F

R

S

R

K

T

T

L

I

V

I

R

K

G

G

A

A

S

S

I

I

G

G

A

A

N

N

A

A

T

T

I

I

V
x
L

C

P

G

G

H

I

E

T

I

I

G

G

E

E

Y

N

A

A

F

M

I

I

G

G

A

A

G

G

A

A

V

I

V

V

T
|
T

R
x
K

D

D

V

V

binding oxidized coenzyme a: C82 (≠ T84), D84 (≠ V86), K85 (≠ N87), Y86 (≠ N88), P87 (= P89), L114 (≠ V117), T132 (= T135), K133 (≠ R136)
binding (3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: F44 (= F46), E46 (≠ G48), D84 (≠ V86), Y92 (≠ S94)

7s3uA Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-amino-3,6-dideoxy-d-glucose (see paper)
49% identity, 71% coverage: 6:138/188 of query aligns to 5:135/145 of 7s3uA

query
sites
7s3uA

H

H

E

K

T

L

A

A

L

D

V

V

D

-

E

Q

G

S

A

K

Q

N

I

I

G

G

E

S

A

G

T

T

R

R

I

I

W

W

H

Q

W

F

A

C

H

V

I

V

C

L

S

P

G

S

A

A

R

I

I

I

G

G

E

E

R

N

C

C

S

N

F

I

G

C

Q

S

N

H

V

C

F
|
F

V

I

G
x
E

N

N

D

D

V

V

L

K

I

I

G

G

N

N

V

V

K

T

V

I

Q

K

N

C

N

G

V

V

S

Q

I

I

Y

W

D

D

A

G

V

I

T

E

L

I

E

E

D

D

V

V

F

F

C

I

G

G

P

P

S

N

M

V

V

T

F

F

T
x
C

N
|
N

V
x
D

N
x
K

N
x
Y

P
|
P

R

R

S

S

A

K

V

Q

S
x
Y

R

P

K

K

H

-

E

E

Y

F

R

S

R

K

T

T

L

I

V

I

R

K

G

G

A

A

S

S

I

I

G

G

A

A

N

N

A

A

T

T

I

I

V
x
L

C

P

G

G

H

I

E

T

I

I

G

G

E

E

Y

N

A

A

F

M

I

I

G

G

A

A

G

G

A

A

V

I

V

V

T
|
T

R
x
K

D

D

V

V

binding coenzyme a: C82 (≠ T84), N83 (= N85), D84 (≠ V86), K85 (≠ N87), Y86 (≠ N88), P87 (= P89), L114 (≠ V117), T132 (= T135), K133 (≠ R136)
binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: F44 (= F46), E46 (≠ G48), D84 (≠ V86), Y92 (≠ S94)

7s45A Crystal structure of an n-acetyltransferase, c80t mutant, from helicobacter pullorum in the presence of acetyl coenzyme a and dtdp (see paper)
48% identity, 71% coverage: 6:138/188 of query aligns to 5:131/141 of 7s45A

query
sites
7s45A

H

H

E

K

T

L

A

A

L

D

V

V

D

-

E

Q

G

S

A

K

Q

N

I

I

G

G

E

S

A

G

T

T

R

R

I

I

W

W

H

Q

W

F

A

C

H

V

I

V

C

L

S

P

G

S

A

A

R

I

I

I

G

G

E

E

R

N

C

C

S

N

F

I

G

C

Q

S

N

H

V

C

F
|
F

V

I

G
x
E

N

N

D

D

V

V

L

K

I

I

G

G

N

N

V

V

K

T

V

I

Q

K

N

C

N

G

V

V

S

Q

I

I

Y
x
W

D

D

A

G

V

I

T

E

L

I

E

E

D

D

V

V

F

F

C

I

G

G

P

P

S

N

M

V

V

T

F

F

T
|
T

N
|
N

V
x
D

N
x
K

N
x
Y

P
|
P

R

R

S

S

A

-

V

-

S

-

R

-

K

-

H

K

E

Q

Y

F

R

S

R

K

T

T

L

I

V

I

R

K

G

G

A

A

S

S

I

I

G

G

A

A

N

N

A

A

T

T

I

I

V
x
L

C

P

G

G

H

I

E

T

I

I

G

G

E

E

Y

N

A

A

F

M

I

I

G

G

A

A

G

G

A

A

V

I

V

V

T
|
T

R
x
K

D

D

V

V

binding acetyl coenzyme *a: W64 (≠ Y66), T82 (= T84), N83 (= N85), D84 (≠ V86), K85 (≠ N87), Y86 (≠ N88), P87 (= P89), L110 (≠ V117), T128 (= T135), K129 (≠ R136)
binding thymidine-5'-diphosphate: F44 (= F46), E46 (≠ G48)

3fsbA Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with coa and dtdp-3-amino-quinovose (see paper)
32% identity, 81% coverage: 17:168/188 of query aligns to 89:234/260 of 3fsbA

query
sites
3fsbA

I

I

G

G

E

D

A

N

T

F

R

Q

I

T

W

G

H

H

W

K

A

V

H

T

I

I

C

R

S

E

G

N

A

T

R

K

I

I

G

G

E

N

R

N

C

V

S

K

F

I

G
|
G

Q

T

N

L

V

S

F

D

V

I

G

Q

N

H

D

H

V

V

L

Y

I

I

G

G

N

N

N

Y

V

V

K

N

V

I

Q
x
H

N

S

N

N

V

V

S

F

I

V

Y

G

D

E

A

K

V

S

T

I

L

I

E

K

D

D

D

F

V

V

F

W

C

L

G
x
F

P

P

S

H

M

V

V

V

F

L

T

T

N

N

V

D

N

P

N

T

P

P

R

P

S

S

A

-

V

-

S

-

R

-

K

-

H

N

E

E

Y

L

R

L

R

G

T

V

L

T

V

I

R

E

R

L

G

F

A

A

S

V

I

I

G
x
A

A
|
A

N

R

A

S

T

V

I

V

V

L

C

P

G

G

H

I

E

H

I

I

G

N

E

E

Y

D

A

A

F

L

I

V

G
|
G

A
|
A

G

G

A

A

V

V

T

T

R

K

D

D

V

V

P

P

A

K

Y

E

A

T

L

V

M

V

V
|
V

G

G

T

N

P

P

A

A

R

R

R

E

I

I

G

C

W

S

M

I

C

R

R

K

C

I

G

K

E

N

R

K

L

I

S

-

T

T

A

G

E

E

S

Q

V

V

binding coenzyme a: F150 (≠ G78), A178 (≠ G111), A179 (= A112), G196 (= G129), A197 (= A130), V212 (= V145)
binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: G114 (= G42), H132 (≠ Q60), F150 (≠ G78)

Sites not aligning to the query:

binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: 78, 241, 243

3fscA Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with coa and dtdp-3-amino-fucose (see paper)
32% identity, 81% coverage: 17:168/188 of query aligns to 89:234/259 of 3fscA

query
sites
3fscA

I

I

G

G

E

D

A

N

T

F

R

Q

I

T

W

G

H

H

W

K

A

V

H

T

I

I

C

R

S

E

G

N

A

T

R

K

I

I

G

G

E

N

R

N

C

V

S
x
K

F

I

G
|
G

Q
x
T

N

L

V

S

F

D

V

I

G

Q

N

H

D

H

V

V

L

Y

I

I

G

G

N

N

N

Y

V

V

K

N

V

I

Q
x
H

N
x
S

N

N

V

V

S

F

I

V

Y

G

D

E

A

K

V

S

T

I

L

I

E

K

D

D

D

F

V

V

F

W

C

L

G
x
F

P

P

S

H

M

V

V

V

F

L

T

T

N

N

V

D

N

P

N

T

P

P

R

P

S

S

A

-

V

-

S

-

R

-

K

-

H

N

E

E

Y

L

R

L

R

G

T

V

L

T

V

I

R

E

R

L

G

F

A

A

S

V

I

I

G
x
A

A
|
A

N

R

A

S

T

V

I

V

V

L

C

P

G

G

H

I

E

H

I

I

G

N

E

E

Y

D

A

A

F

L

I

V

G
|
G

A
|
A

G

G

A

A

V

V

T

T

R

K

D

D

V

V

P

P

A

K

Y

E

A

T

L

V

M

V

V
|
V

G

G

T

N

P

P

A

A

R

R

R

E

I

I

G

C

W

S

M

I

C

R

R

K

C

I

G

K

E

N

R
x
K

L

I

S

-

T

T

A

G

E

E

S

Q

V

V

binding coenzyme a: F150 (≠ G78), A178 (≠ G111), A179 (= A112), G196 (= G129), A197 (= A130), V212 (= V145), K228 (≠ R161)
binding (3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: K112 (≠ S40), G114 (= G42), T115 (≠ Q43), H132 (≠ Q60), S133 (≠ N61)

Sites not aligning to the query:

binding (3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: 78, 243

3fs8A Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d- glucose n-acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with acetyl-coa (see paper)
32% identity, 81% coverage: 17:168/188 of query aligns to 89:234/259 of 3fs8A

query
sites
3fs8A

I

I

G

G

E

D

A

N

T

F

R

Q

I

T

W

G

H

H

W

K

A

V

H

T

I

I

C
x
R

S

E

G

N

A

T

R

K

I

I

G

G

E

N

R

N

C

V

S

K

F

I

G

G

Q

T

N

L

V

S

F

D

V

I

G

Q

N

H

D

H

V

V

L

Y

I

I

G

G

N

N

N

Y

V

V

K

N

V

I

Q

H

N

S

N

N

V

V

S

F

I

V

Y

G

D

E

A

K

V

S

T

I

L

I

E

K

D

D

D

F

V

V

F

W

C

L

G
x
F

P
|
P

S

H

M

V

V

V

F

L

T

T

N

N

V

D

N

P

N

T

P

P

R

P

S

S

A

-

V

-

S

-

R

-

K

-

H

N

E

E

Y

L

R

L

R

G

T

V

L

T

V

I

R

E

R

L

G

F

A

A

S

V

I

I

G
x
A

A
|
A

N

R

A

S

T

V

I

V

V

L

C

P

G

G

H

I

E

H

I

I

G

N

E

E

Y

D

A

A

F

L

I

V

G
|
G

A
|
A

G

G

A

A

V

V

T

T

R

K

D

D

V

V

P

P

A

K

Y

E

A

T

L

V

M

V

V
|
V

G

G

T

N

P

P

A

A

R

R

R

E

I

I

G

C

W

S

M

I

C

R

R

K

C

I

G

K

E

N

R
x
K

L

I

S

-

T

T

A

G

E

E

S

Q

V

V

binding acetyl coenzyme *a: F150 (≠ G78), P151 (= P79), A178 (≠ G111), A179 (= A112), G196 (= G129), A197 (= A130), V212 (= V145), K228 (≠ R161)
binding thymine: R102 (≠ C30)

Sites not aligning to the query:

binding thymine: 84

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
33% identity, 56% coverage: 48:152/188 of query aligns to 72:181/190 of 5u2kA

query
sites
5u2kA

G

G

N

W

D

N

V

V

L

K

I

L

G

G

N

K

N

N

V

V

K

Y

V

V

Q

N

N

T

N

N

V

C

S

Y

I

F

Y
x
M

D

D

A

G

-

G

-

Q

V

I

T

T

L

I

E

G

D

D

D

N

V

V

F

F

C

I

G

G

P

P

S

N

M

C

V

G

F

F

T
x
Y

N

T

V
x
A

N

T

N
x
H

P

P

R

L

S

N

A

F

V

H

S

H

R

R

K

N

H

E

E

G

Y

F

R

E

R

K

T

A

-

G

L

P

V

I

R

H

R

I

G

G

A

S

S

N

-

T

-

W

I

F

G

G

A

G

N

H

A

V

T

A

I

V

V
x
L

C

P

G

G

H

V

E

T

I

I

G

G

E

E

Y

G

A

S

F

V

I

I

G

G

A

A

G

G

A

S

V

V

T
|
T

R
x
K

D

D

V

I

P

P

A

P

Y

H

A

S

L

L

M

A

V

V

G

G

T

N

P

P

A

C

R

K

R

V

I

V

binding coenzyme a: M90 (≠ Y66), Y110 (≠ T84), A112 (≠ V86), H114 (≠ N88), L146 (≠ V117), T164 (= T135), K165 (≠ R136)

Q5KW03 Carbonic anhydrase; Gamma-carbonic anhydrase; Cag; EC 4.2.1.1 from Geobacillus kaustophilus (strain HTA426) (see paper)
30% identity, 72% coverage: 16:150/188 of query aligns to 11:138/182 of Q5KW03

query
sites
Q5KW03

Q

Q

I

I

G

A

E

A

A

S

T

A

R

F

I

I

W

A

H

D

W

Y

A

V

H

T

I

I

C

T

S

G

G

D

A

V

R

V

I

I

G

G

E

E

R

E

C

T

S

S

F

I

G

W

Q

F

N

N

V

T

F

V

V

I

G
x
R

N

G

D

D

V

V

-

A

-

P

-

T

L

V

I

I

G

G

N

N

N

R

V

V

K
x
N

V

I

Q
|
Q

N

D

N

N

V

S

S

I

I

L

Y
x
H

-

Q

-

S

-

P

-

N

D

N

A

P

V

L

T

I

L

I

E

E

D

D

D

G

V

V

F

T

C

V

G

G

P
x
H

S

Q

M

V

V

I

F

L

T
x
H

N

S

V

-

N

-

P

-

R

-

S

-

A

-

V

-

S

-

R

-

K

-

H

-

E

-

Y

-

R

-

T

A

L

I

V

V

R

R

R

K

G

N

A

A

S

L

I

I

G

G

A

M

N

G

A

S

T

I

I

I

V

L

C

D

G

R

H

A

E

E

I

I

G

G

E

E

Y

G

A

A

F

F

I

I

G

G

A

A

G

G

A

S

V

L

V

V

T

P

-

P

-

G

R

K

D

K

V

I

P

P

A

P

Y

N

A

T

L

L

M

A

V

L

G

G

T

R

P

P

A

A

R

K

R43 (≠ G48) mutation to A: Retains 45% of wild-type activity.
N56 (≠ K58) mutation to A: Retains 65% of wild-type activity.
Q58 (= Q60) mutation to A: Retains 20% of wild-type activity.
H64 (≠ Y66) binding ; mutation to A: Retains 20% of wild-type activity.
H81 (≠ P79) binding
H86 (≠ T84) binding

Sites not aligning to the query:

158 Y→A: Retains 10% of wild-type activity.

3vnpA Crystal structure of hypothetical protein (gk2848) from geobacillus kaustophilus
30% identity, 72% coverage: 16:150/188 of query aligns to 12:139/171 of 3vnpA

query
sites
3vnpA

Q

Q

I

I

G

A

E

A

A

S

T

A

R

F

I

I

W

A

H

D

W

Y

A

V

H

T

I

I

C

T

S

G

G

D

A

V

R

V

I

I

G

G

E

E

R

E

C

T

S

S

F

I

G

W

Q

F

N

N

V

T

F

V

V

I

G

R

N

G

D

D

V

V

-

A

-

P

-

T

L

V

I

I

G

G

N

N

N

R

V

V

K

N

V

I

Q

Q

N

D

N

N

V

S

S

I

I

L

Y
x
H

-

Q

-

S

-

P

-

N

D

N

A

P

V

L

T

I

L

I

E

E

D

D

D

G

V

V

F

T

C

V

G

G

P

H

S

Q

M

V

V

I

F

L

T
x
H

N

S

V

-

N

-

P

-

R

-

S

-

A

-

V

-

S

-

R

-

K

-

H

-

E

-

Y

-

R

-

T

A

L

I

V

V

R

R

R

K

G

N

A

A

S

L

I

I

G

G

A

M

N

G

A

S

T

I

I

I

V

L

C

D

G

R

H

A

E

E

I

I

G

G

E

E

Y

G

A

A

F

F

I

I

G

G

A

A

G

G

A

S

V

L

V

V

T

P

-

P

-

G

R

K

D

K

V

I

P

P

A

P

Y

N

A

T

L

L

M

A

V

L

G

G

T

R

P

P

A

A

R

K

binding magnesium ion: H65 (≠ Y66), H87 (≠ T84)

1ssqD Serine acetyltransferase- complex with cysteine (see paper)
32% identity, 65% coverage: 29:150/188 of query aligns to 140:238/257 of 1ssqD

query
sites
1ssqD

I

I

C

H

S

P

G

A

A

A

R

K

I

I

G

G

E

H

R

G

C

I

S

M

F

F

G
x
D

Q
x
H

N

-

V

-

F

-

V

-

G

A

N

T

D

G

V

I

L

V

I

V

G

G

N

E

N

T

V

S

K

V

V

I

Q

E

N

N

N

D

V

V

S

S

I

I

Y

L

D

Q

A

G

V

V

T

T

L

L

E

-

D

-

V

-

F

-

C

-

G

-

P

-

S

-

M

-

V

-

F

-

T

-

N

-

V

-

N

-

P

-

R

-

S

G

A

G

V

T

S

G

R

K

K

E

H

S

E

G

Y

D

R

R

R

H

T

P

L

K

V

V

R

R

R

E

G

G

A

V

S

M

I

I

G

G

A

A

N

G

A

A

T

K

I

I

V

L

C

G

G

N

H

I

E

E

I

V

G

G

E

K

Y

Y

A

A

F

K

I

I

G

G

A

A

G

N

A

S

V

V

T

L

R

N

D

P

V

V

P

P

A

E

Y

Y

A

A

L

T

M

A

V

A

G

G

T

V

P

P

A

A

R

R

binding cysteine: D153 (≠ G42), H154 (≠ Q43)

Sites not aligning to the query:

binding cysteine: 88

3gvdI Crystal structure of serine acetyltransferase cyse from yersinia pestis
34% identity, 55% coverage: 51:153/188 of query aligns to 165:248/272 of 3gvdI

query
sites
3gvdI

V

I

L

V

I

I

G

G

N

E

N

T

V

A

K

V

V

V

Q

E

N

N

N

D

V

V

S

S

I

I

Y

L

D

Q

A

S

V

V

T

T

L

L

E

-

D

-

V

-

F

-

C

-

G

-

P

-

S

-

M

-

V

-

F

-

T

-

N

-

V

-

N

-

P

-

R

-

S
x
G

A
x
G

V

T

S

G

R

K

K

T

H

S

E

G

Y

D

R
|
R

R

H

T

P

L

K

V

I

R

R

R

E

G

G

A

V

S

M

I

I

G

G

A

A

N

G

A

A

T

K

I

I

V

L

C

G

G

N

H

I

E

E

I

V

G

G

E

R

Y

G

A

A

F

K

I

I

G

G

A

A

G

G

A

S

V

V

T

L

R

Q

D

S

V

V

P

P

A

A

Y

H

A

T

L

T

M

A

V

A

G

G

T

V

P

P

A

A

R

R

R

I

I

V

G

G

binding cysteine: G186 (≠ S91), G187 (≠ A92), R195 (= R100)

Sites not aligning to the query:

binding cysteine: 95, 96, 160

3nz2C Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
31% identity, 60% coverage: 39:150/188 of query aligns to 66:176/183 of 3nz2C

query
sites
3nz2C

C

C

S

E

F

F

G

G

Q

K

N

T

V

I

F

R

V

I

G

G

N

D

D

H

V

T

L

F

I

I

G

N

N

M

N

N

V

V

K

V

V

M

Q

L

N

D

N

G

V

A

S

P

I

I

Y

-

D

-

A

-

V

-

T

T

L

I

E

G

D

D

D

H

V

V

F

L

C

I

G

G

P

P

S

S

M

T

V

Q

F

F

T
x
Y

N

T

V

A

N

-

P

-

R

-

S

-

A

-

V

-

S

S

R
x
H

K

S

H

L

E

D

Y

Y

R

R

-

R

-

Q

-

A

-

W

-

E

-

T

-

I

-

C

-

K

-

P

T

I

L

V

V

I

R

E

R

D

G

D

A

V

S

W

I

I

G
|
G

A

G

N

N

A

V

T

V

I

I

V

N

C

Q

G

G

H

V

E

T

I

I

G

G

E

A

Y

R

A

S

F

V

I

V

G
x
A

A
|
A

G
x
N

A

S

V

V

T
x
N

R

Q

D

D

V

V

P

P

A

P

Y

D

A

T

L

L

M

V

V
x
G

G

G

T
|
T

P
|
P

A

A

R

R

binding acetyl coenzyme *a: Y107 (≠ T84), H111 (≠ R95), G137 (= G111), A155 (≠ G129), A156 (= A130), N161 (≠ T135), G171 (≠ V145), T173 (= T147), P174 (= P148)
binding magnesium ion: N157 (≠ G131), T173 (= T147)

Sites not aligning to the query:

binding acetyl coenzyme *a: 178, 179

3ectA Crystal structure of the hexapeptide-repeat containing- acetyltransferase vca0836 from vibrio cholerae
31% identity, 60% coverage: 39:150/188 of query aligns to 59:169/176 of 3ectA

query
sites
3ectA

C

C

S

E

F

F

G

G

Q

K

N

T

V

I

F

R

V

I

G

G

N

D

D

H

V

T

L

F

I

I

G

N

N

M

N

N

V

V

K

V

V

M

Q

L

N

D

N

G

V

A

S

P

I

I

Y

-

D

-

A

-

V

-

T

T

L

I

E

G

D

D

D

H

V

V

F

L

C

I

G

G

P

P

S

S

M

T

V

Q

F

F

T

Y

N

T

V

A

N

-

P

-

R

-

S

-

A

-

V

-

S

S

R

H

K

S

H

L

E

D

Y

Y

R

R

-

R

-

Q

-

A

-

W

-

E

-

T

-

I

-

C

-

K

-

P

T

I

L

V

V

I

R

E

R

D

G

D

A

V

S

W

I

I

G

G

A

G

N

N

A

V

T

V

I

I

V

N

C

Q

G
|
G

H

V

E

T

I

I

G

G

E

A

Y

R

A

S

F

V

I

V

G

A

A

A

G

N

A

S

V

V

T

N

R

Q

D
|
D

V

V

P

P

A

P

Y

D

A

T

L

L

M

V

V

G

G

G

T

T

P

P

A

A

R

R

binding calcium ion: G138 (= G119), D156 (= D137)

Query Sequence

>HSERO_RS20985 FitnessBrowser__HerbieS:HSERO_RS20985
MTYHAHETALVDEGAQIGEATRIWHWAHICSGARIGERCSFGQNVFVGNDVLIGNNVKVQ
NNVSIYDAVTLEDDVFCGPSMVFTNVNNPRSAVSRKHEYRRTLVRRGASIGANATIVCGH
EIGEYAFIGAGAVVTRDVPAYALMVGTPARRIGWMCRCGERLSTAESVTICTECNSRYQI
SDSFCEPL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory