SitesBLAST
Comparing HSERO_RS22305 FitnessBrowser__HerbieS:HSERO_RS22305 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6ihhA Crystal structure of rasadh f12 from ralstonia.Sp in complex with NADPH and a6o
54% identity, 100% coverage: 1:249/249 of query aligns to 1:249/249 of 6ihhA
- binding (2R,3S)-2-ethyl-2-[(2E)-2-(6-methoxy-3,4-dihydro-2H-naphthalen-1-ylidene)ethyl]-3-oxidanyl-cyclopentan-1-one: S137 (= S138), H147 (≠ F148), Y150 (= Y151), L188 (= L189), L246 (≠ M246)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G14), N15 (≠ T16), S16 (= S17), G17 (= G18), I18 (= I19), R38 (= R39), R39 (= R40), D60 (= D61), V61 (≠ A62), N87 (= N88), S88 (≠ A89), G89 (= G90), V110 (≠ T111), T135 (= T136), S137 (= S138), Y150 (= Y151), K154 (= K155), P180 (= P181), G181 (= G182), A182 (≠ P183), I183 (= I184), T185 (= T186), S187 (≠ G188)
4bmsF Short chain alcohol dehydrogenase from ralstonia sp. Dsm 6428 in complex with NADPH
54% identity, 100% coverage: 1:249/249 of query aligns to 1:249/249 of 4bmsF
- active site: S137 (= S138), H147 (≠ F148), Y150 (= Y151), K154 (= K155), Q195 (≠ D195)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G14), N15 (≠ T16), S16 (= S17), I18 (= I19), R38 (= R39), R39 (= R40), A59 (= A60), D60 (= D61), V61 (≠ A62), N87 (= N88), S88 (≠ A89), G89 (= G90), V110 (≠ T111), S137 (= S138), Y150 (= Y151), K154 (= K155), G181 (= G182), I183 (= I184), T185 (= T186), I187 (≠ G188)
4esoB Crystal structure of a putative oxidoreductase protein from sinorhizobium meliloti 1021 in complex with NADP
39% identity, 98% coverage: 4:248/249 of query aligns to 2:246/251 of 4esoB
- active site: G16 (= G18), S136 (= S138), M146 (≠ F148), Y149 (= Y151), K153 (= K155)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G14), T14 (= T16), H15 (≠ S17), M17 (≠ I19), R37 (= R39), N38 (≠ R40), N41 (≠ E43), S58 (≠ A60), D59 (= D61), I60 (≠ A62), N86 (= N88), A87 (= A89), G88 (= G90), T134 (= T136), S136 (= S138), Y149 (= Y151), P179 (= P181), G180 (= G182), I182 (= I184), T184 (= T186), T186 (≠ G188), K187 (≠ L189), G188 (= G190)
5t2uA Short chain dehydrogenase/reductase family protein (see paper)
42% identity, 98% coverage: 2:245/249 of query aligns to 1:237/241 of 5t2uA
- active site: G17 (= G18), T135 (≠ S138), T145 (≠ F148), Y148 (= Y151), K152 (= K155)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G14), G17 (= G18), R38 (= R39), D39 (≠ R40), R42 (≠ E43), D60 (= D61), L61 (= L65), N83 (= N88), A84 (= A89), Y87 (≠ M95), I133 (≠ T136), T135 (≠ S138), Y148 (= Y151), K152 (= K155), P178 (= P181), P180 (= P183), T181 (≠ I184), T183 (= T186), T185 (≠ G188), T186 (≠ L189)
7v0hG Crystal structure of putative glucose 1-dehydrogenase from burkholderia cenocepacia in complex with NADP and a potential reaction product
40% identity, 98% coverage: 4:246/249 of query aligns to 8:252/253 of 7v0hG
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G14), S20 (≠ T16), K21 (≠ S17), G22 (= G18), I23 (= I19), A43 (≠ G38), S44 (≠ R39), S45 (≠ R40), G68 (≠ A60), D69 (= D61), V70 (≠ A62), N96 (= N88), S97 (≠ A89), G98 (= G90), Y100 (≠ G92), I144 (≠ T136), S146 (= S138), Y159 (= Y151), K163 (= K155), P189 (= P181), G190 (= G182), M191 (≠ P183), I192 (= I184), T194 (= T186), G196 (= G188), T197 (≠ L189)
- binding (2R)-2-(hydroxymethyl)pentanedioic acid: S146 (= S138), Y159 (= Y151), M191 (≠ P183), I202 (≠ L192)
6ci9D Rmm microcompartment-associated aminopropanol dehydrogenase NADP + aminoacetone holo-structure (see paper)
38% identity, 98% coverage: 1:245/249 of query aligns to 3:248/259 of 6ci9D
- active site: G20 (= G18), S145 (= S137), Y159 (= Y151)
- binding 1-aminopropan-2-one: F97 (≠ M95), S145 (= S137), T147 (= T139), W156 (≠ F148), Y159 (= Y151), G190 (= G182)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G16 (= G14), S18 (≠ T16), G20 (= G18), I21 (= I19), G40 (= G38), R41 (= R39), N42 (≠ R40), D66 (= D61), V67 (≠ A62), N93 (= N88), G95 (= G90), T143 (≠ L135), S145 (= S137), Y159 (= Y151), K163 (= K155), P189 (= P181), N191 (≠ P183), I192 (= I184), T194 (= T186), G196 (= G188), L197 (= L189)
5fffA Noroxomaritidine/norcraugsodine reductase in complex with NADP+ and piperonal (see paper)
35% identity, 97% coverage: 5:245/249 of query aligns to 9:252/257 of 5fffA
- active site: K206 (≠ Q199)
- binding 1,3-benzodioxole-5-carbaldehyde: Y100 (≠ G92), H158 (≠ F148)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G14), T20 (= T16), G22 (= G18), I23 (= I19), R43 (= R39), C67 (≠ A60), D68 (= D61), V69 (≠ A62), N96 (= N88), I146 (≠ T136), Y161 (= Y151), K165 (= K155), P191 (= P181), A193 (≠ P183), I194 (= I184), T196 (= T186), G198 (= G188), T199 (≠ L189)
5ff9B Noroxomaritidine/norcraugsodine reductase in complex with NADP+ and tyramine (see paper)
35% identity, 97% coverage: 5:245/249 of query aligns to 9:252/257 of 5ff9B
- active site: K206 (≠ Q199)
- binding 4-(2-aminoethyl)phenol: Y100 (≠ G92), I155 (≠ T145), H158 (≠ F148)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G14), T20 (= T16), K21 (≠ S17), I23 (= I19), S42 (≠ G38), R43 (= R39), C67 (≠ A60), D68 (= D61), V69 (≠ A62), N96 (= N88), I146 (≠ T136), S148 (= S138), Y161 (= Y151), K165 (= K155), P191 (= P181), A193 (≠ P183), I194 (= I184), T196 (= T186), G198 (= G188), T199 (≠ L189)
A0A1A9TAK5 Noroxomaritidine/norcraugsodine reductase; NorRed; EC 1.1.1.- from Narcissus pseudonarcissus (Daffodil) (see paper)
35% identity, 97% coverage: 5:245/249 of query aligns to 9:252/257 of A0A1A9TAK5
8hfkA Crystal structure of cbar mutant (h162f) in complex with NADP+ and halogenated aryl ketone (see paper)
36% identity, 98% coverage: 4:247/249 of query aligns to 5:259/259 of 8hfkA
- binding 2-bromanyl-1-(4-bromanyl-2-oxidanyl-phenyl)ethanone: S143 (= S138), N144 (≠ T139), T145 (= T140), F153 (= F148), Y156 (= Y151), G187 (= G182), M193 (≠ G188), V197 (vs. gap), A259 (= A247)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G14), R18 (≠ S17), I20 (= I19), A40 (vs. gap), N41 (vs. gap), S42 (vs. gap), D66 (= D61), N93 (= N88), S94 (≠ A89), L116 (≠ T111), T141 (= T136), Y156 (= Y151), K160 (= K155), P186 (= P181), G187 (= G182), G188 (≠ P183), T189 (≠ I184), T191 (= T186), M193 (≠ G188)
4fj2B Crystal structure of the ternary complex between a fungal 17beta- hydroxysteroid dehydrogenase (holo form) and biochanin a (see paper)
36% identity, 98% coverage: 4:247/249 of query aligns to 5:260/260 of 4fj2B
- active site: G19 (= G18), S143 (= S138), N144 (≠ T139), H154 (≠ F148), Y157 (= Y151), K161 (= K155), Y202 (vs. gap)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G14), R18 (≠ S17), G19 (= G18), I20 (= I19), A40 (≠ I36), N41 (≠ T37), S42 (≠ G38), I67 (≠ A62), N93 (= N88), S94 (≠ A89), G95 (= G90), L116 (≠ T111), T141 (= T136), Y157 (= Y151), K161 (= K155), G188 (= G182), G189 (≠ P183), T190 (≠ I184), T192 (= T186), M194 (≠ G188)
- binding 5,7-dihydroxy-3-(4-methoxyphenyl)-4H-chromen-4-one: G189 (≠ P183), F195 (≠ L189), V198 (≠ L192), S199 (vs. gap), Y202 (vs. gap), I203 (≠ V193), M217 (≠ L201), A218 (≠ L205)
3qwiA Crystal structure of a 17beta-hydroxysteroid dehydrogenase (holo form) from fungus cochliobolus lunatus in complex with NADPH and coumestrol (see paper)
36% identity, 98% coverage: 4:247/249 of query aligns to 5:260/260 of 3qwiA
- active site: G19 (= G18), S143 (= S138), N144 (≠ T139), H154 (≠ F148), Y157 (= Y151), K161 (= K155), Y202 (vs. gap)
- binding Coumestrol: F149 (≠ L143), G189 (≠ P183), M194 (≠ G188), Y202 (vs. gap), I203 (≠ V193), A218 (≠ L205)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G14), R18 (≠ S17), I20 (= I19), A40 (≠ I36), N41 (≠ T37), S42 (≠ G38), I67 (≠ A62), N93 (= N88), S94 (≠ A89), G95 (= G90), L116 (≠ T111), T141 (= T136), Y157 (= Y151), K161 (= K155), P187 (= P181), G188 (= G182), G189 (≠ P183), T190 (≠ I184), T192 (= T186), M194 (≠ G188)
3qwhA Crystal structure of the 17beta-hydroxysteroid dehydrogenase from cochliobolus lunatus in complex with NADPH and kaempferol (see paper)
36% identity, 98% coverage: 4:247/249 of query aligns to 5:260/260 of 3qwhA
- active site: G19 (= G18), S143 (= S138), N144 (≠ T139), H154 (≠ F148), Y157 (= Y151), K161 (= K155), Y202 (vs. gap)
- binding 3,5,7-trihydroxy-2-(4-hydroxyphenyl)-4h-chromen-4-one: N144 (≠ T139), F149 (≠ L143), G189 (≠ P183), F195 (≠ L189), S199 (vs. gap), Y202 (vs. gap), I203 (≠ V193), A218 (≠ L205)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G14), R18 (≠ S17), G19 (= G18), I20 (= I19), A40 (≠ I36), N41 (≠ T37), S42 (≠ G38), D66 (= D61), I67 (≠ A62), N93 (= N88), S94 (≠ A89), G95 (= G90), L116 (≠ T111), T141 (= T136), Y157 (= Y151), K161 (= K155), P187 (= P181), G188 (= G182), G189 (≠ P183), T190 (≠ I184), T192 (= T186), M194 (≠ G188)
4fj1B Crystal structure of the ternary complex between a fungal 17beta- hydroxysteroid dehydrogenase (holo form) and genistein (see paper)
36% identity, 98% coverage: 4:247/249 of query aligns to 4:259/259 of 4fj1B
- active site: G18 (= G18), S142 (= S138), N143 (≠ T139), H153 (≠ F148), Y156 (= Y151), K160 (= K155), Y201 (vs. gap)
- binding genistein: G188 (≠ P183), F194 (≠ L189), S198 (vs. gap), Y201 (vs. gap), I202 (≠ V193), M216 (≠ L201), A217 (≠ L205)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G14), R17 (≠ S17), G18 (= G18), I19 (= I19), A39 (≠ I36), N40 (≠ T37), S41 (≠ G38), I66 (≠ A62), N92 (= N88), S93 (≠ A89), G94 (= G90), L115 (≠ T111), T140 (= T136), S142 (= S138), Y156 (= Y151), K160 (= K155), G187 (= G182), T189 (≠ I184), T191 (= T186), M193 (≠ G188)
4fj0D Crystal structure of the ternary complex between a fungal 17beta- hydroxysteroid dehydrogenase (holo form) and 3,7-dihydroxy flavone (see paper)
36% identity, 98% coverage: 4:247/249 of query aligns to 6:261/261 of 4fj0D
- active site: G20 (= G18), S144 (= S138), N145 (≠ T139), H155 (≠ F148), Y158 (= Y151), K162 (= K155), Y203 (vs. gap)
- binding 3,7-dihydroxy-2-phenyl-4H-chromen-4-one: S144 (= S138), N145 (≠ T139), G190 (≠ P183), F196 (≠ L189), S200 (vs. gap), Y203 (vs. gap), A219 (≠ L205)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G16 (= G14), R19 (≠ S17), G20 (= G18), I21 (= I19), A41 (≠ I36), N42 (≠ T37), S43 (≠ G38), I68 (≠ A62), N94 (= N88), S95 (≠ A89), G96 (= G90), L117 (≠ T111), T142 (= T136), Y158 (= Y151), K162 (= K155), P188 (= P181), G189 (= G182), G190 (≠ P183), T191 (≠ I184), T193 (= T186), M195 (≠ G188)
7ejhA Crystal structure of kred mutant-f147l/l153q/y190p/l199a/m205f/m206f and 2-hydroxyisoindoline-1,3-dione complex
37% identity, 98% coverage: 1:245/249 of query aligns to 2:249/253 of 7ejhA
- binding 2-oxidanylisoindole-1,3-dione: S144 (= S138), I145 (≠ T139), E146 (≠ T140), Y157 (= Y151), V197 (= V193), F207 (≠ D203)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G14), T17 (= T16), I20 (= I19), R40 (= R39), H41 (≠ R40), D64 (= D61), A65 (= A62), N91 (= N88), A92 (= A89), V114 (≠ T111), M142 (≠ T136), S144 (= S138), Y157 (= Y151), K161 (= K155), P189 (= P181), G190 (= G182), P191 (= P183), I192 (= I184), T194 (= T186), P195 (= P187), L196 (= L192)
1g0nA Structure of trihydroxynaphthalene reductase in complex with NADPH and 4,5,6,7-tetrachloro-phthalide (see paper)
35% identity, 97% coverage: 5:245/249 of query aligns to 17:270/273 of 1g0nA
- active site: G30 (= G18), S154 (= S138), H165 (≠ F148), Y168 (= Y151), K172 (= K155)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G26 (= G14), R29 (≠ S17), G30 (= G18), I31 (= I19), A51 (vs. gap), N52 (vs. gap), S53 (≠ T37), V78 (≠ A62), N104 (= N88), S105 (≠ A89), G106 (= G90), I127 (≠ T111), M152 (≠ T136), Y168 (= Y151), K172 (= K155), P198 (= P181), G200 (≠ P183), I201 (= I184), T203 (= T186), M205 (≠ G188)
- binding 4,5,6,7-tetrachloro-phthalide: S154 (= S138), Y168 (= Y151), G200 (≠ P183), M205 (≠ G188), Y206 (≠ L189), C210 (vs. gap), Y213 (≠ L192), W233 (≠ V209)
1dohA Structure of trihydroxynaphthalene reductase in complex with NADPH and 4-nitro-inden-1-one (see paper)
35% identity, 97% coverage: 5:245/249 of query aligns to 17:270/273 of 1dohA
- active site: G30 (= G18), S154 (= S138), H165 (≠ F148), Y168 (= Y151), K172 (= K155), Y213 (≠ L192)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G26 (= G14), R29 (≠ S17), G30 (= G18), I31 (= I19), A51 (vs. gap), N52 (vs. gap), S53 (≠ T37), N77 (≠ D61), V78 (≠ A62), N104 (= N88), S105 (≠ A89), G106 (= G90), M152 (≠ T136), Y168 (= Y151), K172 (= K155), P198 (= P181), G200 (≠ P183), I201 (= I184), T203 (= T186), M205 (≠ G188)
- binding 4-nitro-inden-1-one: Y168 (= Y151), G200 (≠ P183), Y206 (≠ L189), C210 (vs. gap), Y213 (≠ L192)
1g0oC Structure of trihydroxynaphthalene reductase in complex with NADPH and pyroquilon (see paper)
35% identity, 97% coverage: 5:245/249 of query aligns to 25:278/281 of 1g0oC
- active site: G38 (= G18), S162 (= S138), H173 (≠ F148), Y176 (= Y151), K180 (= K155), Y221 (≠ L192)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G34 (= G14), R37 (≠ S17), G38 (= G18), I39 (= I19), A59 (vs. gap), N60 (vs. gap), S61 (≠ T37), N85 (≠ D61), V86 (≠ A62), N112 (= N88), S113 (≠ A89), G114 (= G90), M160 (≠ T136), Y176 (= Y151), K180 (= K155), P206 (= P181), G208 (≠ P183), I209 (= I184), T211 (= T186), M213 (≠ G188)
- binding pyroquilon: S162 (= S138), I163 (≠ T139), Y176 (= Y151), G208 (≠ P183), Y221 (≠ L192)
Q12634 Tetrahydroxynaphthalene reductase; T4HN reductase; THNR; EC 1.1.1.252 from Pyricularia oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Magnaporthe oryzae) (see 2 papers)
35% identity, 97% coverage: 5:245/249 of query aligns to 27:280/283 of Q12634
- 39:63 (vs. 17:37, 32% identical) binding
- Y178 (= Y151) active site, Proton acceptor
Query Sequence
>HSERO_RS22305 FitnessBrowser__HerbieS:HSERO_RS22305
MNKQLNGKIALVTGGTSGIGLATAQELAAQGAQVFITGRRQAELDAAVASIGAAATGIRA
DASVLSELDTVYAQIAKSAGRLDILFANAGGGDMMPLGAITEEHFDRIFGTNVRGVLFTV
QKALPLLVDGASVILTSSTTSVLGTANFSVYSASKAAVRNFARSWALDLKDRAIRVNVVS
PGPIRTPGLGELVPDDARQGLFDYLASQVPLGRLGEPQEIGKAVAFLASDAASFVNGIEL
FVDGGMAQV
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory