SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS22745 FitnessBrowser__HerbieS:HSERO_RS22745 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

P94530 Arabinooligosaccharides transport system permease protein AraQ from Bacillus subtilis (strain 168) (see paper)
27% identity, 86% coverage: 43:300/300 of query aligns to 23:280/281 of P94530

query
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P94530

A

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K

D180 (= D200) mutation to A: Negative impact in the growth rate in the presence of arabinotriose.

P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
28% identity, 70% coverage: 92:300/300 of query aligns to 82:295/296 of P68183

query
sites
P68183

L

L

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Y

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W

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E190 (≠ D195) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A197) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G201) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P214) mutation to A: No effect.

P02916 Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12) (see 4 papers)
36% identity, 20% coverage: 158:216/300 of query aligns to 364:422/514 of P02916

query
sites
P02916

D

D

L

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L372 (= L166) mutation to W: Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose.
N376 (≠ Y170) mutation N->K,H: No growth on maltose.
G380 (= G174) mutation G->C,S: No growth on maltose.
E401 (≠ D195) mutation E->A,C,K,L: Reduction of transport rate.
S403 (≠ A197) mutation S->C,D,K,L: Reduction of transport rate.
G407 (= G201) mutation G->A,P: No effect.
P420 (= P214) mutation to A: No effect.

Sites not aligning to the query:

334 L→W: Ability to transport lactose in a saturable manner.

4ki0F Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose (see paper)
36% identity, 20% coverage: 158:216/300 of query aligns to 349:407/490 of 4ki0F

query
sites
4ki0F

D

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L

binding alpha-D-glucopyranose: N361 (≠ Y170), G365 (= G174), Y368 (≠ L177)

Sites not aligning to the query:

binding alpha-D-glucopyranose: 310, 418, 422

Query Sequence

>HSERO_RS22745 FitnessBrowser__HerbieS:HSERO_RS22745
MLASSPLSTSSTSATIMRKPTLIQRSFGQAGHLWVHTVLCLYAVIALFPIALILINSVKT
RNAIFEGPMALPTAETLTFAGFQKVLAGTHFLLYFGNSLAVTVAALFLIVLFGAMAAWAL
TEYRFFGNRALNFFIAIGIMIPIRLGTVSILQLVVALDLINTRTALVLVYTAQGLPLAVM
ILSEFMRQIPGELKDAARCDGVGELSIFFRVILPLLRPAIATVAVFTMIPAWNDLWFPLI
LAPGEDTRTVTLGVQQFIGQYATDWNSVLAALSMAVIPVLLLYMAFSRQLIRGLTSGAVK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory