SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS22810 FitnessBrowser__HerbieS:HSERO_RS22810 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 14 hits to proteins with known functional sites (download)

Q47945 Alcohol dehydrogenase (quinone), cytochrome c subunit; ADH; Alcohol dehydrogenase (quinone), subunit II; Cytochrome c-553; Cytochrome c553; Ethanol:Q2 reductase; G3-ADH subunit II; Quinohemoprotein-cytochrome c complex; Ubiquinol oxidase; EC 1.1.5.5 from Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) (see paper)
44% identity, 98% coverage: 3:416/423 of query aligns to 19:434/478 of Q47945

query
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Q47945

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Q37 (= Q25) modified: Pyrrolidone carboxylic acid

Sites not aligning to the query:

1:36 signal peptide

8gy2B Cryo-em structure of membrane-bound alcohol dehydrogenase from gluconobacter oxydans
44% identity, 92% coverage: 27:416/423 of query aligns to 1:396/433 of 8gy2B

query
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8gy2B

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binding heme c: C18 (= C44), C21 (= C47), H22 (= H48), T46 (= T72), I48 (= I74), Y59 (= Y85), L68 (≠ V94), R73 (= R99), V79 (≠ L105), Y80 (= Y106), M83 (= M109), F88 (≠ Y114), R126 (= R152), H165 (= H190), C166 (= C191), C169 (= C194), H170 (= H195), I201 (≠ A224), A202 (≠ V225), P203 (= P226), L205 (= L228), W216 (= W234), F224 (≠ Y242), A234 (≠ S252), V235 (= V253), F236 (≠ A254), F236 (≠ A254), M239 (= M257), N301 (= N322), C302 (= C323), C305 (= C326), H306 (= H327), M316 (≠ I337), F317 (= F338), P318 (= P339), L320 (= L341), P324 (≠ T345), G342 (≠ A363), S352 (= S373), V354 (≠ L375), M356 (= M377), F359 (= F380), M375 (≠ V396)
binding ubiquinone-10: C21 (= C47), L34 (= L60), P39 (≠ G65), P81 (= P107), L129 (≠ M155), W132 (= W158), E168 (≠ S193), R173 (= R198), I197 (≠ L220), D241 (≠ L259)

Sites not aligning to the query:

binding heme c: 428

8jejC Cryo-em structure of na-dithionite reduced membrane-bound fructose dehydrogenase from gluconobacter japonicus
39% identity, 91% coverage: 32:418/423 of query aligns to 1:402/413 of 8jejC

query
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8jejC

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V

L

M
|
M

P

P

G

A

F
|
F

-

G

-

P

-

D

-

S

-

L

A

V

H

Q

R

P

L

L

S

T

D

D

E

E

E

Q

A

I

A

A

A

T

L
x
I

A

A

T

D

F

Y

V

V

R

L

Q

S

G

H

W

F

G

G

N

N

H

A

A

Q

G

A

K

T

V

V

S

S

E

A

R

D

E

A

V

V

G

K

K

Q

L

V

R

R

A

A

G

G

binding heme c: C13 (= C44), C16 (= C47), H17 (= H48), T42 (= T72), I44 (= I74), F60 (= F90), L64 (≠ V94), L75 (= L105), Y76 (= Y106), M79 (= M109), P80 (= P110), Y84 (= Y114), R122 (= R152), C162 (= C191), C165 (= C194), H166 (= H195), I186 (≠ L220), W189 (= W223), A191 (≠ V225), P192 (= P226), I194 (≠ L228), W205 (= W234), Y213 (= Y242), R223 (≠ S252), M228 (= M257), V303 (≠ N322), C304 (= C323), C307 (= C326), H308 (= H327), Y320 (≠ F338), P321 (= P339), L323 (= L341), T327 (= T345), T328 (≠ V346), D336 (≠ A354), I341 (= I359), V345 (≠ A363), R347 (= R364), I354 (≠ L375), M356 (= M377), F359 (= F380), I376 (≠ L392)
binding ubiquinone-10: M36 (≠ H66), P77 (= P107), S124 (≠ G154), W128 (= W158), C165 (= C194), L173 (≠ M202)

Sites not aligning to the query:

binding ubiquinone-10: 408, 411

7w2jC Cryo-em structure of membrane-bound fructose dehydrogenase from gluconobacter japonicus
39% identity, 91% coverage: 32:418/423 of query aligns to 1:388/418 of 7w2jC

query
sites
7w2jC

V

I

A

S

R

R

G

G

Q

H

Y

Y

L

L

A

A

R

I

A

A

G

A

D

D

C
|
C

M

A

A

A

C
|
C

H
|
H

S

T

A

N

A

G

G

R

K

D

P

G

A

Q

Y

F

-

L

S

A

G

G

L

Y

A

A

I

I

D

S

S

S

G

P

H

M

G

G

I

N

I

I

Y

Y

S

S

T
|
T

N

N

I
|
I

T

T

P

P

D

S

K

K

E

T

H

H

G

G

I

I

G

G

N

N

Y
|
Y

S

T

E

L

A

E

Q

Q

F

F

S

S

A

K

A

A

V

L

R

R

H

H

G

G

V

I

R

R

A

A

D

D

G

G

T

A

Q

Q

L
|
L

Y
|
Y

P

P

A
|
A

M
|
M

P

P

Y

Y

P

D

S

A

Y

Y

A

N

K

R

V

L

S

T

D

D

E

E

D

D

I

V

H

K

A

S

L

L

Y

Y

T

A

Y

Y

F

I

M

M

Q

T

G

E

V

V

Q

K

P

P

V

V

A

D

S

A

T

P

P

S

P

P

A

K

S

T

S

Q

M

L

S

P

F

F

P

P

F

F

N

S

I

I

R
|
R

W

A

G

S

M

L

K

G

L

I

W

W

N

K

V

I

F

A

A

R

N

I

D

E

-

G

K

K

P

P

F

Y

R

V

E

F

Q

D

D

H

G

T

W

H

S

N

A

D

E

D

I

W

K

N

R

R

G

G

A

R

Y

Y

L

L

V

V

E

D

G

E

L

L

G

A

H
|
H

C
|
C

G

G

S

E

C
|
C

H
|
H

T

T

P

P

R

R

G

N

V

F

A

L

M

L

N

A

E

P

K

-

A

-

S

-

D

-

S

-

S

N

Q

Q

A

S

Q

A

F

Y

L

L

S

A

G

G

G

A

D

D

L

I

N

G

G

S

W

W

A

R

V
x
A

P
|
P

S

N

L

I

R

T

G

N

M

A

P

P

H

Q

-

S

-

G

-

I

-

G

-

S

W

W

S

S

A

D

Q

Q

D

D

I

L

V

F

D

Q

Y

Y

L

L

Q

K

T

T

G

G

R

K

N

T

K

A

T

H

A

A

S
x
R

V

A

A

A

G

G

E
x
P

M
|
M

S

A

L

E

V

A

V

I

E

E

H

H

S

S

T

L

S

Q

H

Y

L

L

K

P

R

D

E

A

D

D

L

I

Q

S

A

A

M

I

A

V

A

T

Y

Y

L

L

K

R

T

S

L

V

S

P

P

-

V

-

A

A

S

K

S

A

G

E

S

S

G

G

R

Q

V

T

I

V

P

A

Q

N

G

F

V

E

D

H

A

A

T

-

V

-

S

G

K

R

L

P

T

S

A

S

A

Y

K

S

D

V

L

T

T

T

L

D

G

G

E

A

R

A

L

L

Y

Y

L

E

D

A

N
x
V

C
|
C

A

A

A

S

C
|
C

H
|
H

F

Q

V

S

T

D

G

G

R

K

G

G

A

S

P

K

-

D

G

G

I
x
Y

F
x
Y

P
|
P

V

S

L
|
L

D

V

G

G

A
x
N

T
|
T

V
x
T

V

T

N

G

A

Q

E

L

N

N

P

P

S

N

A
x
D

L

L

L

I

H

A

V

S

I
|
I

L

L

A

Y

G

G

A
x
V

-

D

R
|
R

T

T

P

T

S

D

T

N

E

H

K

E

A

-

P

-

S

-

I

-

L
x
I

V

L

M
|
M

P
|
P

G

A

F
|
F

-

G

-

P

-

D

-

S

-

L

A

V

H

Q

R

P

L

L

S

T

D

D

E

E

E

Q

A

I

A

A

A

T

L

I

A

A

T

D

F

Y

V

V

R

L

Q

S

G

H

W

F

G

G

N

N

H

A

A

Q

G

A

K

T

V

V

S

S

E

A

R

D

E

A

V

V

G

K

K

Q

L

V

R

R

A

A

G

G

binding heme c: C13 (= C44), C16 (= C47), H17 (= H48), T42 (= T72), I44 (= I74), Y55 (= Y85), L75 (= L105), Y76 (= Y106), A78 (= A108), M79 (= M109), R122 (= R152), H161 (= H190), C162 (= C191), C165 (= C194), H166 (= H195), A191 (≠ V225), P192 (= P226), R223 (≠ S252), P227 (≠ E256), M228 (= M257), V289 (≠ N322), C290 (= C323), C293 (= C326), H294 (= H327), Y305 (≠ I337), Y306 (≠ F338), P307 (= P339), L309 (= L341), N312 (≠ A344), T313 (= T345), T314 (≠ V346), D322 (≠ A354), I327 (= I359), V331 (≠ A363), R333 (= R364), I340 (≠ L375), M342 (= M377), P343 (= P378), F345 (= F380)

8gy3A Cryo-em structure of membrane-bound aldehyde dehydrogenase from gluconobacter oxydans
34% identity, 92% coverage: 8:398/423 of query aligns to 16:409/440 of 8gy3A

query
sites
8gy3A

F

F

A

L

A

A

L

Y

A

A

W

W

S

-

L

Y

G

P

A

A

L

I

G

A

T

P

L

I

T

P

-

R

-

P

T

A

A

A

Q

S

A

S

A

F

D

S

Q

A

D

D

L

A

V

I

A

S

R

R

G

G

Q

E

Y

I

L

V

A

A

R

N

A

G

G

G

D
x
Y

C
|
C

M

A

A
x
E

C
|
C

H
|
H

S

T

A

R

A

V

-

D

G

G

K

K

P

P

A

G

-

P

-

E

Y

L

S

A

G

G

G

D

L

F

A

K

I

M

D

A

S
x
T

G

P

H
x
F

G

G

I

D

I

I

Y

F

S

S

T
x
S

N

N

I
|
I

T

T

P

P

D

D

K

E

E

E

H

W

G

G

I

I

G

G

N

N

Y
x
W

S

S

E

L

A

A

Q

A

F
|
F

S

K

A

R

A

A

V

M

R

N

H

K

G

G

V

I

R

A

A

R

D

D

G

G

T

S

Q

Q

L
|
L

Y
|
Y

P
|
P

A

A

M
x
F

P

P

Y

F

P

D

S

H

Y
x
F

A

T

K

K

V

V

S

S

D

D

E

Q

D

D

I

V

H

S

A

D

L

L

Y

Y

T

A

Y

Y

F

L

M

M

Q

-

G

-

V

T

Q

R

P

P

-

A

V

V

A

H

S

L

T

K

P

P

P

R

A

D

S

N

S

T

M

V

S

P

F

F

P

P

F
x
I

N

N

I

I

R
|
R

W

L

-

I

G
|
G

M
x
Q

K

G

L
x
F

W
|
W

N

K

V

L

F

L

F

F

A

F

N

T

D

P

K

G

P

R

F

Y

R

Q

E

N

Q

D

D

P

G

K

W

H

S

D

A

A

E

Q

I

W

K

N

R

R

G

G

A

A

Y

Y

L

L

V

A

E

E

G

G

L

N

G

E

H
|
H

C
|
C

G

G

S

A

C
|
C

H
|
H

T

T

P

P

R
|
R

G

N

V

L

A
x
L

M

G

N

A

E

E

K

K

A

M

S

S

D

-

S

-

Q

-

A

-

Q

S

F

V

L

Y

S

D

G

G

G

A

D

V

L

I

N

D

G

G

W

W

A

I

V
x
A

P
|
P

S

P

L
|
L

R

N

G

D

M

H

P

N

H

P

-

T

-

P

-

V

W
|
W

S

T

A

E

Q

D

D

E

I

L

V

F

D

Q

Y
|
Y

L
|
L

Q

R

T

F

G

G

R

V

N

A

K

P

T

L

-

H

A
x
G

S
|
S

V

A

A

A

G

G

E

P

M
|
M

S

S

L

P

V

V

V

P

E

H

H

R

S

F

T

L

S

S

H

K

L

I

K

P

R

E

E

E

D

D

L

V

Q

H

A

A

M

I

A

A

A

H

Y

Y

L
x
Y

K

A

T

D

L

V

S

D

P

K

V

A

A

A

-

Q

-

R

S

S

G

G

S

D

G

Q

R

A

V

A

I

I

P

T

Q

R

G

A

V

M

D

Q

A

M

T

S

V

G

S

R

K

D

L

L

T

T

A

G

A

P

K

Q

D

P

L

L

T

D

L

E

G

D

E

A

R

R

L

L

Y

Y

L

Q

D

G

N

A

C
|
C

A

G

A

A

C
|
C

H
|
H

F

Y

V

N

T

S

G

G

R

P

G

N

A

P

P

V

G

L

I

G

F

R

P
|
P

V

E

L
|
L

D

A

G

L

A
x
N

T
x
N

V

A

V

L

N

W

A

L

E

D

N

E

P

P

S

N

A

N

L

L

L

Y

H

Q

V
|
V

I

M

L

L

A

H

G

G

A
x
I

R

-

T

-

P

-

S

T

T

A

E

E

K

E

A
x
G

P
x
Q

S

D

I

H

L
x
I

V

S

M
|
M

P

P

G

S

F
|
F

A

Y

H

S

R

G

L

L

S

S

D

D

E

H

E

D

A

M

A

A

A

R

L

I

A

A

T

A

F

Y

V

L

R

R

Q

R

binding heme c: Y52 (≠ D43), C53 (= C44), C56 (= C47), H57 (= H48), S84 (≠ T72), I86 (= I74), W97 (≠ Y85), F102 (= F90), L117 (= L105), F121 (≠ M109), F126 (≠ Y114), R163 (= R152), C203 (= C191), C206 (= C194), H207 (= H195), A232 (≠ V225), P233 (= P226), L235 (= L228), W245 (= W234), Y253 (= Y242), L254 (= L243), G263 (≠ A251), S264 (= S252), M269 (= M257), Y292 (≠ L280), C337 (= C323), C340 (= C326), H341 (= H327), P353 (= P339), L355 (= L341), N358 (≠ A344), N359 (≠ T345), V372 (= V358), I377 (≠ A363), G382 (≠ A371), Q383 (≠ P372), I386 (≠ L375), M388 (= M377), F391 (= F380)
binding ubiquinone-10: E55 (≠ A46), T76 (≠ S64), F78 (≠ H66), Y118 (= Y106), P119 (= P107), I160 (≠ F149), G166 (= G154), Q167 (≠ M155), F169 (≠ L157), W170 (= W158), H202 (= H190), R210 (= R198), L213 (≠ A201)

8hddB Complex structure of catalytic, small, and a partial electron transfer subunits from burkholderia cepacia fad glucose dehydrogenase (see paper)
45% identity, 24% coverage: 317:416/423 of query aligns to 24:121/121 of 8hddB

query
sites
8hddB

R

R

L

L

Y

Y

L

L

D

G

N

N

C
|
C

A

A

A

T

C
|
C

H
|
H

F

Q

V

M

T

Q

G

G

R

K

G

G

A

T

P

P

-

D

G

G

I

Y

F
x
Y

P
|
P

V

S

L

L

D

F

G

H

A

N

T

S

V
x
T

V

V

N

G

A

A

E

S

N

N

P

P

S

S

A

N

L

L

L

V

H

Q

V
|
V

I
|
I

L

L

A

N

G

G

A

V

-

Q

R
|
R

T

K

P

I

S

G

T

S

E

E

K

D

A

-

P

-

S

-

I

-

L
x
I

V

G

M
|
M

P
|
P

G

A

F

F

A

R

H

Y

R

D

L

L

S

N

D

D

E

A

E

Q

A

I

A

A

L

L

A

T

T

N

F

Y

V

V

R

T

Q

A

G

Q

W

F

G

G

N

N

H

P

A

A

G

A

K

K

V

V

S

T

E

E

R

Q

E

D

V

V

G

A

K

K

L

L

R

R

binding protoporphyrin ix containing fe: C30 (= C323), C33 (= C326), H34 (= H327), Y46 (≠ F338), P47 (= P339), T54 (≠ V346), V66 (= V358), I67 (= I359), R73 (= R364), I80 (≠ L375), M82 (= M377), P83 (= P378)

2zooA Crystal structure of nitrite reductase from pseudoalteromonas haloplanktis tac125
38% identity, 28% coverage: 297:416/423 of query aligns to 318:438/438 of 2zooA

query
sites
2zooA

Q

Q

G

S

V

L

D

D

A

N

T

T

V

F

S

T

K

K

L

I

T

T

A

A

A

N

-

N

-

K

-

D

K

E

D

Q

L

I

T

R

L

F

G

G

E

Q

R

R

L

V

Y

Y

L

E

D

A

N

N

C
|
C

A

M

A

A

C
|
C

H
|
H

F

Q

V

A

T

N

G

G

R

E

G

G

A

I

P

P

G

G

I

A

F
|
F

P
|
P

V
x
P

L
|
L

D

A

G

K

A
x
S

T

D

V
x
Y

V

L

N

N

A

-

E

N

N

N

P

P

S

L

A

L

L

G

L

V

H

N

V

A

I
|
I

L

I

A

K

G

G

A
x
L

R
x
S

T
x
G

P

P

-
x
I

S

K

T
x
V

E

N

K

N

A

V

P

N

S
x
Y

I
x
N

L
x
G

V
|
V

M
|
M

P

P

G

-

F

-

A

A

H

M

R

N

L

L

S

N

D

D

E

E

E

D

A

I

A

A

A

N

L

V

A

I

T

T

F

F

V

V

R

L

Q

N

G

N

W

W

G

D

N

N

H

A

A

G

G

G

K

K

V

V

S

S

E

A

R

E

E

Q

V

V

G

A

K

K

L

Q

R

R

binding protoporphyrin ix containing fe: C347 (= C323), C350 (= C326), H351 (= H327), F362 (= F338), P363 (= P339), P364 (≠ V340), L365 (= L341), S368 (≠ A344), Y370 (≠ V346), I382 (= I359), L386 (≠ A363), S387 (≠ R364), G388 (≠ T365), I390 (vs. gap), V392 (≠ T368), Y397 (≠ S373), N398 (≠ I374), G399 (≠ L375), V400 (= V376), M401 (= M377)

Sites not aligning to the query:

active site: 81, 84, 86, 121, 122, 130, 135, 227, 249, 250, 276
binding copper (ii) ion: 81, 86, 121, 122, 130, 135

4ax3D Structure of three-domain heme-cu nitrite reductase from ralstonia pickettii at 1.6 a resolution (see paper)
31% identity, 29% coverage: 295:417/423 of query aligns to 334:456/457 of 4ax3D

query
sites
4ax3D

I

V

P

T

Q

K

G

A

V

T

D

Q

A

A

T

S

V

V

S

S

-

G

K

T

L

L

T

T

A

V

A

Q

K

D

D

Q

L

V

T

Q

L

A

G

G

E

R

R

A

L

L

Y

F

L

A

D

G

N

T

C
|
C

A

S

A

V

C
|
C

H
|
H

F

Q

V

G

T

N

G

G

R

A

G

G

A

L

P

P

G

G

I

V

F

F

P
|
P

V
x
P

L
|
L

D

A

G

K

A
x
S

T

D

V
x
F

V

L

N

-

A

A

E

A

N

D

P

P

S

K

A

R

L

A

L

M

H

N

V

I

I

V

L

L

A

H

G

G

A

L

R
x
N

T
x
G

P

K

S

I

T

K

E

V

K

N

A

G

P

Q

S

E

I

Y

-

D

L
x
S

V

V

M
|
M

P

P

G

P

F
x
M

A

T

H

-

R

Q

L

L

S

N

D

D

E

D

E

E

A

V

A

A

A

N

L

I

A

L

T

T

F

Y

V

V

R

L

Q

N

G

S

W

W

G

D

N

N

H

P

A

G

G

G

K

R

V

V

S

S

E

A

R

E

E

D

V

V

G

K

K

K

L

V

R

R

A

A

binding heme c: C363 (= C323), C366 (= C326), H367 (= H327), P379 (= P339), P380 (≠ V340), L381 (= L341), S384 (≠ A344), F386 (≠ V346), N403 (≠ R364), G404 (≠ T365), S415 (≠ L375), M417 (= M377), M420 (≠ F380)

Sites not aligning to the query:

active site: 93, 96, 98, 133, 134, 142, 147, 239, 261, 262, 288
binding copper (ii) ion: 93, 98, 133, 134, 142, 147

5oboA Crystal structure of nitrite bound d97n mutant of three-domain heme-cu nitrite reductase from ralstonia pickettii
31% identity, 29% coverage: 295:417/423 of query aligns to 332:454/456 of 5oboA

query
sites
5oboA

I

V

P

T

Q

K

G

A

V

T

D

Q

A

A

T

S

V

V

S

S

-

G

K

T

L

L

T

T

A

V

A

Q

K

D

D

Q

L

V

T

Q

L

A

G

G

E

R

R

A

L

L

Y

F

L

A

D

G

N
x
T

C
|
C

A

S

A

V

C
|
C

H
|
H

F

Q

V

G

T

N

G

G

R

A

G

G

A

L

P

P

G

G

I

V

F

F

P
|
P

V
x
P

L
|
L

D

A

G

K

A
x
S

T

D

V
x
F

V

L

N

-

A

A

E

A

N

D

P

P

S

K

A

R

L

A

L

M

H

N

V
x
I

I

V

L

L

A

H

G

G

A

L

R
x
N

T
x
G

P

K

S

I

T

K

E

V

K

N

A

G

P

Q

S

E

I

Y

-

D

L
x
S

V

V

M
|
M

P

P

G

P

F
x
M

A

T

H

-

R

Q

L

L

S

N

D

D

E

D

E

E

A

V

A

A

A

N

L

I

A

L

T

T

F

Y

V

V

R

L

Q

N

G

S

W

W

G

D

N

N

H

P

A

G

G

G

K

R

V

V

S

S

E

A

R

E

E

D

V

V

G

K

K

K

L

V

R

R

A

A

binding heme c: T360 (≠ N322), C361 (= C323), C364 (= C326), H365 (= H327), P377 (= P339), P378 (≠ V340), L379 (= L341), S382 (≠ A344), F384 (≠ V346), I395 (≠ V358), N401 (≠ R364), G402 (≠ T365), S413 (≠ L375), M415 (= M377), M418 (≠ F380)

Sites not aligning to the query:

active site: 91, 94, 96, 131, 132, 140, 145, 237, 259, 260, 286
binding copper (ii) ion: 91, 96, 131, 132, 140, 145
binding nitrite ion: 94, 96, 131

6fjaA Crystal structure of t2d three-domain heme-cu nitrite reductase from ralstonia pickettii
31% identity, 29% coverage: 295:417/423 of query aligns to 331:453/455 of 6fjaA

query
sites
6fjaA

I

V

P

T

Q

K

G

A

V

T

D

Q

A

A

T

S

V

V

S

S

-

G

K

T

L

L

T

T

A

V

A

Q

K

D

D

Q

L

V

T

Q

L

A

G

G

E

R

R

A

L

L

Y

F

L

A

D

G

N
x
T

C
|
C

A

S

A

V

C
|
C

H
|
H

F

Q

V

G

T

N

G

G

R

A

G

G

A

L

P

P

G

G

I

V

F

F

P
|
P

V
x
P

L
|
L

D

A

G

K

A

S

T

D

V
x
F

V

L

N

-

A

A

E

A

N

D

P

P

S

K

A

R

L

A

L

M

H

N

V

I

I

V

L

L

A

H

G

G

A

L

R
x
N

T
x
G

P

K

S

I

T

K

E

V

K

N

A

G

P

Q

S

E

I
x
Y

-

D

L
x
S

V

V

M
|
M

P

P

G

P

F
x
M

A

T

H

-

R

Q

L

L

S

N

D

D

E

D

E

E

A

V

A

A

A

N

L

I

A

L

T

T

F

Y

V

V

R

L

Q

N

G

S

W

W

G

D

N

N

H

P

A

G

G

G

K

R

V

V

S

S

E

A

R

E

E

D

V

V

G

K

K

K

L

V

R

R

A

A

binding protoporphyrin ix containing fe: T359 (≠ N322), C360 (= C323), C363 (= C326), H364 (= H327), P376 (= P339), P377 (≠ V340), L378 (= L341), F383 (≠ V346), N400 (≠ R364), G401 (≠ T365), Y410 (≠ I374), S412 (≠ L375), M414 (= M377), M417 (≠ F380)

Sites not aligning to the query:

active site: 90, 93, 95, 130, 131, 139, 144, 236, 258, 259, 285
binding copper (ii) ion: 90, 131, 139, 144

1dt1A Thermus thermophilus cytochrome c552 synthesized by escherichia coli (see paper)
37% identity, 24% coverage: 315:417/423 of query aligns to 2:111/129 of 1dt1A

query
sites
1dt1A

G

G

E

A

R

K

L

I

Y

Y

L

A

D

-

N

Q

C
|
C

A

A

A

G

C
|
C

H
|
H

F

Q

V

Q

T

N

G

G

R

Q

G

G

A

I

P

P

G

G

I

A

F

F

P
|
P

V

P

L

L

D

A

G

G

-
x
H

-

V

A

A

T

E

V

I

V

L

N

A

A

K

E

E

N

G

P

G

S

R

A

E

-
x
Y

L

L

L

I

H

L

V
|
V

I

L

L

L

A

Y

G

G

A

L

R
x
Q

T
x
G

P

Q

S

I

T

E

E

V

K

K

A

G

P

M

S

K

I

Y

L

N

-
x
G

V

V

M
|
M

P

S

G

S

F
|
F

A

A

H

-

R

Q

L

L

S

K

D

D

E

E

A

I

A

A

L

V

A

L

T

N

F

H

V

I

R

A

Q

T

G

A

W

W

G

G

N

D

H

-

A

A

G

K

K

K

V

V

-

K

-

G

-

F

-

K

-

P

-

F

S

T

E

A

R

E

E

E

V

V

G

K

K

K

L

L

R

R

A

A

binding heme c: C9 (= C323), C12 (= C326), H13 (= H327), P25 (= P339), H30 (vs. gap), Y43 (vs. gap), V47 (= V358), Q53 (≠ R364), G54 (≠ T365), G65 (vs. gap), M67 (= M377), F70 (= F380)

Sites not aligning to the query:

binding heme c: 123

1r0qA Characterization of the conversion of the malformed, recombinant cytochrome rc552 to a 2-formyl-4-vinyl (spirographis) heme (see paper)
37% identity, 24% coverage: 315:417/423 of query aligns to 3:112/130 of 1r0qA

query
sites
1r0qA

G

G

E

A

R

K

L

I

Y

Y

L

A

D

-

N

Q

C

C

A

A

A

G

C
|
C

H
|
H

F

Q

V

Q

T

N

G

G

R

Q

G

G

A

I

P

P

G

G

I

A

F

F

P
|
P

V

P

L
|
L

D

A

G

G

-
x
H

-

V

A

A

T

E

V

I

V

L

N

A

A

K

E

E

N

G

P

G

S

R

A

E

-
x
Y

L

L

L

I

H

L

V
|
V

I

L

L

L

A

Y

G

G

A

L

R
x
Q

T
x
G

P

Q

S

I

T

E

E

V

K

K

A

G

P

M

S

K

I

Y

L

N

-
x
G

V

V

M
|
M

P

S

G

S

F

F

A

A

H

-

R

Q

L

L

S

K

D

D

E

E

A

I

A

A

L

V

A

L

T

N

F

H

V

I

R

A

Q

T

G

A

W

W

G

G

N

D

H

-

A

A

G

K

K

K

V

V

-

K

-

G

-

F

-

K

-

P

-

F

S

T

E

A

R

E

E

E

V

V

G

K

K

K

L

L

R

R

A

A

binding 2-formyl-protoporphryn ix: C13 (= C326), H14 (= H327), P26 (= P339), L28 (= L341), H31 (vs. gap), Y44 (vs. gap), V48 (= V358), Q54 (≠ R364), G55 (≠ T365), G66 (vs. gap), M68 (= M377)

Sites not aligning to the query:

binding 2-formyl-protoporphryn ix: 124

1qyzA Characterization of the malformed, recombinant cytochrome rc552 (see paper)
37% identity, 24% coverage: 315:417/423 of query aligns to 3:112/130 of 1qyzA

query
sites
1qyzA

G

G

E

A

R

K

L

I

Y
|
Y

L

A

D

-

N

Q

C
|
C

A

A

A

G

C
|
C

H
|
H

F

Q

V

Q

T

N

G

G

R

Q

G

G

A

I

P

P

G

G

I

A

F

F

P
|
P

V

P

L

L

D

A

G

G

-
x
H

-

V

A

A

T

E

V

I

V

L

N

A

A

K

E

E

N

G

P

G

S

R

A

E

-
x
Y

L

L

L

I

H

L

V

V

I

L

L

L

A

Y

G

G

A

L

R
x
Q

T
x
G

P

Q

S

I

T

E

E

V

K

K

A

G

P

M

S

K

I

Y

L

N

-
x
G

V

V

M
|
M

P

S

G

S

F
|
F

A

A

H

-

R

Q

L

L

S

K

D

D

E

E

A

I

A

A

L

V

A

L

T

N

F

H

V

I

R

A

Q

T

G

A

W

W

G

G

N

D

H

-

A

A

G

K

K

K

V

V

-

K

-

G

-

F

-

K

-

P

-

F

S

T

E

A

R

E

E

E

V

V

G

K

K

K

L

L

R

R

A

A

binding 2-acetyl-protoporphyrin ix: Y7 (= Y319), C10 (= C323), C13 (= C326), H14 (= H327), P26 (= P339), H31 (vs. gap), Y44 (vs. gap), Q54 (≠ R364), G55 (≠ T365), G66 (vs. gap), M68 (= M377), F71 (= F380)

Sites not aligning to the query:

binding 2-acetyl-protoporphyrin ix: 124

1c52A Thermus thermophilus cytochrome-c552: a new highly thermostable cytochromE-C structure obtained by mad phasing (see paper)
37% identity, 24% coverage: 315:417/423 of query aligns to 4:113/131 of 1c52A

query
sites
1c52A

G

G

E

A

R

K

L

I

Y

Y

L

A

D

-

N

Q

C
|
C

A

A

A

G

C
|
C

H
|
H

F

Q

V

Q

T

N

G

G

R

Q

G

G

A

I

P

P

G

G

I

A

F
|
F

P
|
P

V

P

L
|
L

D

A

G

G

-
x
H

-

V

A

A

T

E

V

I

V

L

N

A

A

K

E

E

N

G

P

G

S

R

A

E

-
x
Y

L

L

L

I

H

L

V

V

I

L

L

L

A

Y

G

G

A
x
L

R
x
Q

T
x
G

P

Q

S

I

T

E

E

V

K

K

A

G

P

M

S

K

I

Y

L

N

-
x
G

V

V

M
|
M

P

S

G

S

F
|
F

A

A

H

-

R

Q

L

L

S

K

D

D

E

E

A

I

A

A

L

V

A

L

T

N

F

H

V

I

R

A

Q

T

G

A

W

W

G

G

N

D

H

-

A

A

G

K

K

K

V

V

-

K

-

G

-

F

-

K

-

P

-

F

S

T

E

A

R

E

E

E

V

V

G

K

K

K

L

L

R

R

A

A

binding protoporphyrin ix containing fe: C11 (= C323), C14 (= C326), H15 (= H327), F26 (= F338), P27 (= P339), L29 (= L341), H32 (vs. gap), Y45 (vs. gap), L54 (≠ A363), Q55 (≠ R364), G56 (≠ T365), G67 (vs. gap), M69 (= M377), F72 (= F380)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 125

Query Sequence

>HSERO_RS22810 FitnessBrowser__HerbieS:HSERO_RS22810
MKKLTSLFAALAWSLGALGTLTTAQAADQDLVARGQYLARAGDCMACHSAAGKPAYSGGL
AIDSGHGIIYSTNITPDKEHGIGNYSEAQFSAAVRHGVRADGTQLYPAMPYPSYAKVSDE
DIHALYTYFMQGVQPVASTPPASSMSFPFNIRWGMKLWNVFFANDKPFREQDGWSAEIKR
GAYLVEGLGHCGSCHTPRGVAMNEKASDSSQAQFLSGGDLNGWAVPSLRGMPHWSAQDIV
DYLQTGRNKTASVAGEMSLVVEHSTSHLKREDLQAMAAYLKTLSPVASSGSGRVIPQGVD
ATVSKLTAAKDLTLGERLYLDNCAACHFVTGRGAPGIFPVLDGATVVNAENPSALLHVIL
AGARTPSTEKAPSILVMPGFAHRLSDEEAAALATFVRQGWGNHAGKVSEREVGKLRAGMD
KQH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory