SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS23040 FitnessBrowser__HerbieS:HSERO_RS23040 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q7CRQ0 Uronate dehydrogenase; D-galacturonate dehydrogenase; D-glucuronate dehydrogenase; Hexuronate dehydrogenase; EC 1.1.1.203 from Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) (see paper)
51% identity, 92% coverage: 15:274/284 of query aligns to 1:257/265 of Q7CRQ0

query
sites
Q7CRQ0

F

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Y134 (= Y152) mutation to A: 0.1% of wild-type activity.

3rfvA Crystal structure of uronate dehydrogenase from agrobacterium tumefaciens complexed with nadh and product (see paper)
51% identity, 92% coverage: 15:274/284 of query aligns to 2:258/265 of 3rfvA

query
sites
3rfvA

F

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binding D-galactaro-1,5-lactone: S74 (= S91), S110 (= S127), N111 (= N128), H112 (= H129), Y135 (= Y152), S164 (= S181), R173 (= R190), F257 (= F273)
binding 1,4-dihydronicotinamide adenine dinucleotide: G9 (= G22), G12 (= G25), Q13 (≠ G26), L14 (≠ V27), D33 (= D46), L34 (= L47), D50 (= D67), L51 (= L68), L70 (= L87), G71 (= G88), G72 (= G89), S74 (= S91), A108 (= A125), S110 (= S127), Y135 (= Y152), K139 (= K156), I162 (= I179), S164 (= S181), C165 (≠ I182)

3rfxA Crystal structure of uronate dehydrogenase from agrobacterium tumefaciens, y136a mutant complexed with NAD (see paper)
51% identity, 92% coverage: 15:274/284 of query aligns to 2:258/265 of 3rfxA

query
sites
3rfxA

F

M

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K

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R

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L

L

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G

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A

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binding nicotinamide-adenine-dinucleotide: G9 (= G22), G12 (= G25), Q13 (≠ G26), L14 (≠ V27), D33 (= D46), L34 (= L47), D50 (= D67), L51 (= L68), G72 (= G89), S74 (= S91)

3a4vA Crystal structure of pyruvate bound l-threonine dehydrogenase from hyperthermophilic archaeon thermoplasma volcanium (see paper)
27% identity, 58% coverage: 18:183/284 of query aligns to 2:168/311 of 3a4vA

query
sites
3a4vA

L

I

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active site: T112 (≠ S127), Y137 (= Y152), K141 (= K156)
binding nicotinamide-adenine-dinucleotide: S8 (≠ A24), G9 (= G25), Q10 (≠ G26), I11 (≠ V27), D32 (≠ A50), I33 (≠ M51), L46 (≠ C66), D47 (= D67), V48 (≠ L68), L69 (= L87), A70 (≠ G88), G71 (= G89), L73 (vs. gap), P110 (≠ A125), S111 (= S126), T112 (≠ S127), Y137 (= Y152), K141 (= K156), Y164 (≠ I179), I167 (= I182)
binding pyruvic acid: S74 (vs. gap), Y137 (= Y152)

Sites not aligning to the query:

binding pyruvic acid: 178, 179, 273

3a1nA Crystal structure of l-threonine dehydrogenase from hyperthermophilic archaeon thermoplasma volcanium (see paper)
27% identity, 58% coverage: 18:183/284 of query aligns to 2:168/315 of 3a1nA

query
sites
3a1nA

L

I

L

L

L

V

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T

G

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L

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Q

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I

active site: T112 (≠ S127), Y137 (= Y152), K141 (= K156)
binding nicotinamide-adenine-dinucleotide: S8 (≠ A24), G9 (= G25), Q10 (≠ G26), I11 (≠ V27), D32 (≠ A50), I33 (≠ M51), L46 (≠ C66), D47 (= D67), L69 (= L87), A70 (≠ G88), G71 (= G89), L73 (vs. gap), S111 (= S126), Y137 (= Y152), K141 (= K156), Y164 (≠ I179)

3ajrA Crystal structure of l-3-hydroxynorvaline bound l-threonine dehydrogenase (y137f) from hyperthermophilic archaeon thermoplasma volcanium (see paper)
26% identity, 58% coverage: 18:183/284 of query aligns to 2:168/314 of 3ajrA

query
sites
3ajrA

L

I

L

L

L

V

T

T

G

G

A

S

A
x
S

G
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G

G
x
Q

V
x
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G

G

K

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Q

E

L

L

R

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A

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Y

L

L

A

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A

Y

E

G

V

K

R

N

V

V

A

-

D

-

L

I

A

A

S

S

A
x
D

M
x
I

A

V

A

Q

V

R

D

D

P

T

A

G

A

I

H

-

E

-

E

K

A

F

L

I

G

T

C
x
L

D
|
D

L
x
V

A

S

D

N

R

R

A

D

A

E

V

I

D

D

A

R

M

A

V

V

A

E

-

K

-

Y

G

S

C

I

E

D

A

A

I

I

I

F

H

H

L
|
L

G
x
A

G
|
G

V

I

-
x
L

-
x
S

-

A

S

K

V

G

E

E

R

K

P

D

F

P

E

A

E

L

I

A

L

Y

E

K

A

V

N

N

I

M

K

N

G

G

V

T

F

Y

H

N

I

I

Y

L

E

E

A

A

R

K

R

Q

H

H

G

R

V

V

K

E

R

K

V

V

I

V

F

I

A
x
P

S
|
S

S
x
T

N
x
I

H

G

V

V

T

F

G

G

F

P

Y

E

G

T

Q

P

D

K

E

N

R

K

I

V

D

P

A

S

R

I

D

T

M

I

K

T

R

R

P

P

D

R

G

T

Y

M

Y
x
F

G

G

L

V

S

T

K
|
K

S

I

Y

A

G

A

E

E

D

L

M

L

A

G

Q

Q

F

Y

Y

Y

F

Y

D

E

R

K

Y

F

G

G

V

L

E

D

T

V

V

R

S

S

I

L

R

R

I
x
Y

G

P

S

G

I
|
I

F

I

binding nicotinamide-adenine-dinucleotide: S8 (≠ A24), G9 (= G25), Q10 (≠ G26), I11 (≠ V27), D32 (≠ A50), I33 (≠ M51), L46 (≠ C66), D47 (= D67), V48 (≠ L68), L69 (= L87), A70 (≠ G88), G71 (= G89), L73 (vs. gap), P110 (≠ A125), S111 (= S126), T112 (≠ S127), F137 (≠ Y152), K141 (= K156), Y164 (≠ I179), I167 (= I182)
binding (3R)-3-hydroxy-L-norvaline: S74 (vs. gap), T112 (≠ S127), I113 (≠ N128), F137 (≠ Y152)

Sites not aligning to the query:

binding (3R)-3-hydroxy-L-norvaline: 177, 178, 179

3a9wA Crystal structure of l-threonine bound l-threonine dehydrogenase (y137f) from hyperthermophilic archaeon thermoplasma volcanium (see paper)
26% identity, 58% coverage: 18:183/284 of query aligns to 2:168/315 of 3a9wA

query
sites
3a9wA

L

I

L

L

L

V

T

T

G

G

A

S

A
x
S

G
|
G

G
x
Q

V
x
I

G

G

K

T

Q

E

L

L

R

V

A

P

R

Y

L

L

A

A

S

E

F

K

A

Y

E

G

V

K

R

N

V

V

A

-

D

-

L

I

A

A

S

S

A
x
D

M
x
I

A

V

A

Q

V

R

D

D

P

T

A

G

A

I

H

-

E

-

E

K

A

F

L

I

G

T

C
x
L

D
|
D

L
x
V

A

S

D

N

R

R

A

D

A

E

V

I

D

D

A

R

M

A

V

V

A

E

-

K

-

Y

G

S

C

I

E

D

A

A

I

I

I

F

H

H

L
|
L

G
x
A

G

G

V

I

-
x
L

-
x
S

-

A

S

K

V

G

E

E

R

K

P

D

F

P

E

A

E

L

I

A

L

Y

E

K

A
x
V

N

N

I

M

K

N

G

G

V

T

F

Y

H

N

I

I

Y

L

E

E

A

A

R

K

R

Q

H

H

G

R

V

V

K

E

R

K

V

V

I

V

F

I

A
x
P

S
|
S

S
x
T

N

I

H

G

V

V

T

F

G

G

F

P

Y

E

G

T

Q

P

D

K

E

N

R

K

I

V

D

P

A

S

R

I

D

T

M

I

K

T

R

R

P

P

D

R

G

T

Y

M

Y
x
F

G

G

L

V

S

T

K
|
K

S

I

Y

A

G

A

E

E

D

L

M

L

A

G

Q

Q

F

Y

Y

Y

F

Y

D

E

R

K

Y

F

G

G

V

L

E

D

T

V

V

R

S

S

I

L

R

R

I
x
Y

G

P

S

G

I
|
I

F

I

binding nicotinamide-adenine-dinucleotide: S8 (≠ A24), G9 (= G25), Q10 (≠ G26), I11 (≠ V27), D32 (≠ A50), I33 (≠ M51), L46 (≠ C66), D47 (= D67), V48 (≠ L68), L69 (= L87), A70 (≠ G88), L73 (vs. gap), V87 (≠ A102), P110 (≠ A125), S111 (= S126), K141 (= K156), Y164 (≠ I179), I167 (= I182)
binding threonine: S74 (vs. gap), T112 (≠ S127), F137 (≠ Y152)

Sites not aligning to the query:

binding threonine: 177, 178, 179

4id9A Crystal structure of a short-chain dehydrogenase/reductase superfamily protein from agrobacterium tumefaciens (target efi-506441) with bound NAD, monoclinic form 1
30% identity, 57% coverage: 18:179/284 of query aligns to 2:159/321 of 4id9A

query
sites
4id9A

L

I

L

L

L

V

T

T

G

G

A

S

A

A

G
|
G

G
x
R

V
|
V

G

G

K

R

Q

A

L

V

R

V

A

A

R

A

L

L

A

R

S

T

F

Q

A

G

E

R

V

T

V

V

R

R

V

G

A

F

D
|
D

L
|
L

A
x
R

S

P

A

S

M

-

A

-

V

-

D

-

P

-

A

G

A

T

A

G

H

G

E

E

A

V

L

V

G

G

C
x
S

D

-

L
|
L

A

E

D

D

R

G

A

Q

A

A

V

L

D

S

A

D

M

A

V

I

A

M

G

G

C

V

E

S

A

A

I

V

I

L

H

H

L
|
L

G

G

G
x
A

V

F

S

M

V

S

E

W

R

A

P

P

F

A

E

D

E

R

-

D

-

R

I

M

L

F

E

A

A
x
V

N

N

I

V

K

E

G

G

V

T

F

R

H

R

I

L

Y

L

E

D

A

A

R

S

R

A

H

A

G

G

V

V

K

R

R

R

V

F

I

V

F

F

A

A

S

S

S
|
S

N

G

H

E

V

V

T

-

G

-

F

-

Y

Y

G

P

Q

E

D

N

E

R

-

P

-

E

-

F

-

L

R

P

I

V

D

T

A

E

R

D

D

H

M

P

K

L

R

C

P

P

D

N

G

S

Y

P

Y
|
Y

G

G

L

L

S

T

K
|
K

S

L

Y

L

G

G

E

E

D

E

M

L

A

V

Q

R

F

F

Y

H

F

Q

D

R

R

S

Y

G

G

A

V

M

E

E

T

T

V

V

S

I

I

L

R

R

I
x
F

active site: S106 (= S127), Y132 (= Y152), K136 (= K156)
binding nicotinamide-adenine-dinucleotide: G9 (= G25), R10 (≠ G26), V11 (= V27), D30 (= D46), L31 (= L47), R32 (≠ A48), S44 (≠ C66), L45 (= L68), L64 (= L87), A66 (≠ G89), V81 (≠ A102), S106 (= S127), Y132 (= Y152), K136 (= K156), F159 (≠ I179)

Sites not aligning to the query:

4id9B Crystal structure of a short-chain dehydrogenase/reductase superfamily protein from agrobacterium tumefaciens (target efi-506441) with bound NAD, monoclinic form 1
30% identity, 57% coverage: 18:179/284 of query aligns to 3:160/328 of 4id9B

query
sites
4id9B

L

I

L

L

L

V

T

T

G

G

A

S

A

A

G
|
G

G
x
R

V
|
V

G

G

K

R

Q

A

L

V

R

V

A

A

R

A

L

L

A

R

S

T

F

Q

A

G

E

R

V

T

V

V

R

R

V

G

A

F

D
|
D

L
|
L

A

R

S

P

A

S

M

-

A

-

V

-

D

-

P

-

A

G

A

T

A

G

H

G

E

E

A

V

L

V

G

G

C
x
S

D

-

L
|
L

A

E

D

D

R

G

A

Q

A

A

V

L

D

S

A

D

M

A

V

I

A

M

G

G

C

V

E

S

A

A

I

V

I

L

H

H

L
|
L

G

G

G
x
A

V

F

S

M

V

S

E

W

R

A

P

P

F

A

E

D

E

R

-

D

-

R

I

M

L

F

E

A

A
x
V

N

N

I

V

K

E

G

G

V

T

F

R

H

R

I

L

Y

L

E

D

A

A

R

S

R

A

H

A

G

G

V

V

K

R

R

R

V

F

I

V

F

F

A

A

S

S

S
|
S

N

G

H

E

V

V

T

-

G

-

F

-

Y

Y

G

P

Q

E

D

N

E

R

-

P

-

E

-

F

-

L

R

P

I

V

D

T

A

E

R

D

D

H

M

P

K

L

R

C

P

P

D

N

G

S

Y

P

Y
|
Y

G

G

L

L

S

T

K
|
K

S

L

Y

L

G

G

E

E

D

E

M

L

A

V

Q

R

F

F

Y

H

F

Q

D

R

R

S

Y

G

G

A

V

M

E

E

T

T

V

V

S

I

I

L

R

R

I
x
F

active site: S107 (= S127), Y133 (= Y152), K137 (= K156)
binding nicotinamide-adenine-dinucleotide: G10 (= G25), R11 (≠ G26), V12 (= V27), D31 (= D46), L32 (= L47), S45 (≠ C66), L46 (= L68), L65 (= L87), A67 (≠ G89), V82 (≠ A102), Y133 (= Y152), K137 (= K156), F160 (≠ I179)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 161, 162, 163

5u4qB 1.5 angstrom resolution crystal structure of NAD-dependent epimerase from klebsiella pneumoniae in complex with NAD.
28% identity, 59% coverage: 17:183/284 of query aligns to 3:181/304 of 5u4qB

query
sites
5u4qB

R

K

L

F

L

L

L

V

T

T

G
|
G

A

A

G
|
G

G
x
F

V
x
I

G

G

K

F

Q

H

L

I

R

A

A

Q

R

R

L

L

A

L

S

N

F

E

A

G

E

H

V

N

V

V

R

V

V

G

A

I

D
|
D

L
x
N

A

M

S
x
N

A

D

M

Y

A
x
Y

A

D

V

V

D

S

P

L

A
x
K

A

Q

A

A

H

R

E

L

E

D

A

R

L

L

G

A

-

Y

-

P

-

A

-

F

-

H

-

F

-

Q

C

L

D
|
D

L
|
L

A

A

D

D

R

R

A

E

A

G

V

M

D

A

A

K

M

L

V

F

A

A

G

T

-

E

-

Q

C

F

E

D

A

R

I

V

I

I

H

H

L
|
L

G
x
A

-
x
A

-

Q

-

A

G

G

V

V

S

R

V

L

E

E

R

N

P

P

F

Y

E

A

E

-

I

Y

L

A

E

D

A

A

N

N

I

L

K

M

G

G

V

Y

F

L

H

N

I

I

Y

L

E

E

A

G

A

C

R

R

R

H

H

T

G

K

V

V

K

K

R

H

V

L

I

V

F

Y

A
|
A

S

S

N

S

H

S

V

V

T

Y

G

G

F

L

Y

N

G

R

Q

K

D

M

E

P

R

F

I

S

D

T

A

E

R

D

D

S

M

V

K

D

R

H

P

P

D

V

G

S

Y

L

Y
|
Y

G

A

L

A

S

T

K
|
K

S

K

Y

A

G

N

E

E

D

L

M

M

A

A

Q

H

F

T

Y

Y

F

S

D

H

R

L

Y

Y

G

G

V

I

E

P

T

T

V

T

S

G

I

L

R

R

I
x
F

G

F

S

T

I
x
V

F

Y

binding nicotinamide-adenine-dinucleotide: G8 (= G22), G11 (= G25), F12 (≠ G26), I13 (≠ V27), D32 (= D46), N33 (≠ L47), N35 (≠ S49), Y38 (≠ A52), K43 (≠ A57), D61 (= D67), L62 (= L68), L83 (= L87), A84 (≠ G88), A85 (vs. gap), A123 (= A125), Y150 (= Y152), K154 (= K156), F177 (≠ I179), V180 (≠ I182)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 186, 189

6wjaA Udp-glcnac c4-epimerase mutant s121a/y146f from pseudomonas protegens in complex with udp-galnac (see paper)
33% identity, 59% coverage: 17:183/284 of query aligns to 2:174/307 of 6wjaA

query
sites
6wjaA

R

R

L

I

L

L

L

V

T

T

G
|
G

A

G

A

A

G
|
G

G
x
F

V
x
I

G

G

K

S

Q

H

L

L

R

V

A

D

R

A

L

L

A

L

S

A

F

K

A

G

E

Y

V

A

V

V

R

R

V

V

A

L

D
|
D

-
x
D

L

L

A
x
S

S
x
T

A
x
G

M

K

A

V

A

G

V

N

D

L

P

P

A

M

A

G

-

D

A

A

H

G

E

L

E

E

A

L

L

L

G

V

C

G

D

D

L
x
A

A

A

D

D

R

A

A

A

A

L

V

L

D

A

A

D

M

A

V

V

A

Q

G

G

C

C

E

D

A

A

I

V

H

H

L
|
L

G
x
A

V

V

-

A

-

S

-
x
V

-

Q

-

A

S

S

V

V

E

E

R

D

P

P

F

V

E

A

E

T

I

-

L

H

E

Q

A
x
S

N

N

I

F

K

I

G

A

V

T

F

L

H

R

I

L

Y

C

E

E

A

A

A

M

R

T

R

A

H

A

G

G

V

I

K

R

R

R

V

V

I

V

F

F

A

A

S

S

S
x
A

N
x
A

H
x
A

V

V

T

Y

G

G

F

N

Y

N

G

G

Q

E

D

G

E

T

R

P

I

I

D

A

A

E

R

D

D

T

M

P

K

K

R

S

P

P

D

L

G

T

Y

P

Y
x
F

G

A

L

A

S

D

K
|
K

S

L

Y

A

G

S

E

E

D

Y

M

Y

A

L

Q

D

F

F

Y

Y

F

R

D

R

R

Q

Y

H

G

G

V

L

E

E

T

P

V

V

S

I

I

L

R

R

I
x
F

G
x
F

S
x
N

I
|
I

F

F

active site: A118 (≠ S127), A119 (≠ N128), A120 (≠ H129), F143 (≠ Y152), K147 (= K156)
binding nicotinamide-adenine-dinucleotide: G7 (= G22), G10 (= G25), F11 (≠ G26), I12 (≠ V27), D31 (= D46), D32 (vs. gap), S34 (≠ A48), T35 (≠ S49), G36 (≠ A50), A55 (≠ L68), L74 (= L87), A75 (≠ G88), A76 (≠ G89), S93 (≠ A102), F143 (≠ Y152), K147 (= K156), F170 (≠ I179), F171 (≠ G180), I173 (= I182)
binding uridine-diphosphate-n-acetylgalactosamine: V80 (vs. gap), A120 (≠ H129), N172 (≠ S181)

Sites not aligning to the query:

binding uridine-diphosphate-n-acetylgalactosamine: 186, 187, 191, 202, 204, 211, 247, 270, 273

6wj9B Udp-glcnac c4-epimerase mutant s121a/y146f from pseudomonas protegens in complex with udp-glcnac (see paper)
33% identity, 59% coverage: 17:183/284 of query aligns to 3:175/308 of 6wj9B

query
sites
6wj9B

R

R

L

I

L

L

L

V

T

T

G
|
G

A

G

A

A

G
|
G

G
x
F

V
x
I

G

G

K

S

Q

H

L

L

R

V

A

D

R

A

L

L

A

L

S

A

F

K

A

G

E

Y

V

A

V

V

R

R

V

V

A

L

D
|
D

-
x
D

L

L

A
x
S

S
x
T

A
x
G

M

K

A

V

A

G

V

N

D

L

P

P

A

M

A

G

-

D

A

A

H

G

E

L

E

E

A

L

L

L

G

V

C

G

D
|
D

L
x
A

A

A

D

D

R

A

A

A

A

L

V

L

D

A

A

D

M

A

V

V

A

Q

G

G

C

C

E

D

A

A

I

V

H

H

L
|
L

G
x
A

V

V

-

A

-

S

-
x
V

-

Q

-

A

S

S

V

V

E

E

R

D

P

P

F

V

E

A

E

T

I

-

L

H

E

Q

A
x
S

N

N

I

F

K

I

G

A

V

T

F

L

H

R

I

L

Y

C

E

E

A

A

A

M

R

T

R

A

H

A

G

G

V

I

K

R

R

R

V

V

I

V

F

F

A
|
A

S

S

S
x
A

N
x
A

H
x
A

V

V

T

Y

G

G

F

N

Y

N

G

G

Q

E

D

G

E

T

R

P

I

I

D

A

A

E

R

D

D

T

M

P

K

K

R

S

P

P

D

L

G

T

Y

P

Y
x
F

G

A

L

A

S

D

K
|
K

S

L

Y

A

G

S

E

E

D

Y

M

Y

A

L

Q

D

F

F

Y

Y

F

R

D

R

R

Q

Y

H

G

G

V

L

E

E

T

P

V

V

S

I

I

L

R

R

I
x
F

G
x
F

S
x
N

I
|
I

F

F

active site: A119 (≠ S127), A120 (≠ N128), A121 (≠ H129), F144 (≠ Y152), K148 (= K156)
binding nicotinamide-adenine-dinucleotide: G8 (= G22), G11 (= G25), F12 (≠ G26), I13 (≠ V27), D32 (= D46), D33 (vs. gap), S35 (≠ A48), T36 (≠ S49), G37 (≠ A50), D55 (= D67), A56 (≠ L68), L75 (= L87), A76 (≠ G88), A77 (≠ G89), S94 (≠ A102), A117 (= A125), A119 (≠ S127), F144 (≠ Y152), K148 (= K156), F171 (≠ I179), F172 (≠ G180), I174 (= I182)
binding uridine-diphosphate-n-acetylglucosamine: V81 (vs. gap), N173 (≠ S181)

Sites not aligning to the query:

binding uridine-diphosphate-n-acetylglucosamine: 187, 188, 192, 203, 204, 205, 212, 248, 271, 274

6jygD Crystal structure of l-threonine dehydrogenase from phytophthora infestans (see paper)
28% identity, 60% coverage: 16:186/284 of query aligns to 1:171/307 of 6jygD

query
sites
6jygD

S

S

R

R

L

I

L

L

L

V

T

T

G
|
G

A

G

A
x
T

G
|
G

G
x
Q

V
x
I

G

G

K

M

Q

E

L

L

R

V

A

P

R

Y

L

M

A

R

S

Q

F

L

-

F

-

G

A

A

E

D

V

T

V

I

R

V

V

N

A

S

D
|
D

L
x
I

A
x
K

S

A

A

P

M

-

A

-

V

-

D

-

P

-

A

-

A

G

A

R

H

D

E

G

E

N

A

F

L

V

G

Y

C

C

D
|
D

L
x
V

A

Q

D

D

R

R

A

D

A

S

V

M

D

A

A

R

M

I

V

V

A

T

-

E

-

Q

G

G

C

V

E

D

A

T

I

I

I

V

H

H

-
x
M

-
x
A

-
x
S

-

L

L
|
L

G
x
S

G

A

V

V

S

G

V

E

E

A

R

N

P

P

F

-

E

S

E

L

I

A

L

L

E

S

A
x
V

N

N

I

T

K

R

G

G

V

I

F

Q

H

N

I

V

Y

L

E

E

A

L

A

A

R

K

R

Q

H

Y

G

Q

V

L

K

R

R

-

V

-

I

V

F

F

A

A

S
x
P

S
|
S

N
x
T

H

-

V

-

T

I

G

A

F

V

Y

F

G

G

-

P

-

S

-

T

-

P

Q

Q

D

D

E

E

R

T

I

P

D

D

A

T

R

T

D

I

M

M

K

-

R

R

P

P

D

T

G

T

Y

M

Y
|
Y

G

G

L

L

S

T

K
|
K

S

V

Y

H

G

V

E

E

D

L

M

L

A

G

Q

E

F

Y

Y

Y

F

Y

D

N

R

K

Y

F

G

G

V

V

E

D

T

F

V

R

S

S

I

V

R

R

I
x
Y

-

P

G

G

S
x
V

I

I

F

S

P

S

E

E

A

A

binding citrate anion: Q11 (≠ G26), L73 (= L87), S74 (≠ G88), Y136 (= Y152)
binding nicotinamide-adenine-dinucleotide: G7 (= G22), T9 (≠ A24), G10 (= G25), Q11 (≠ G26), I12 (≠ V27), D33 (= D46), I34 (≠ L47), K35 (≠ A48), D47 (= D67), V48 (≠ L68), M69 (vs. gap), A70 (vs. gap), S71 (vs. gap), L73 (= L87), V87 (≠ A102), P109 (≠ S126), S110 (= S127), T111 (≠ N128), Y136 (= Y152), K140 (= K156), Y163 (≠ I179), V166 (≠ S181)

Sites not aligning to the query:

binding citrate anion: 176, 178, 179

5u4qA 1.5 angstrom resolution crystal structure of NAD-dependent epimerase from klebsiella pneumoniae in complex with NAD.
27% identity, 59% coverage: 17:183/284 of query aligns to 3:176/321 of 5u4qA

query
sites
5u4qA

R

K

L

F

L

L

L

V

T

T

G
|
G

A

A

G
|
G

G
x
F

V
x
I

G

G

K

F

Q

H

L

I

R

A

A

Q

R

R

L

L

A

L

S

N

F

E

A

G

E

H

V

N

V

V

R

V

V

G

A

I

D
|
D

L
x
N

A

M

S
x
N

A

D

M

Y

A
x
Y

A

D

V

V

D

S

P

L

A
x
K

A

Q

A

A

H

R

E

L

E

D

A

R

L

L

G

A

-

Y

-

P

-

A

-

F

-

H

-

F

-

Q

C

L

D
|
D

L
|
L

A

A

D

D

R

R

A

E

A

G

V

M

D

A

A

K

M

L

V

F

A

A

G

T

-

E

-

Q

C

F

E

D

A

R

I

V

I

I

H

H

L
|
L

G
x
A

V

Q

S

A

V

-

E

-

R

N

P

P

F

Y

E

A

E

-

I

Y

L

A

E

D

A

A

N

N

I

L

K

M

G

G

V

Y

F

L

H

N

I

I

Y

L

E

E

A

G

A

C

R

R

R

H

H

T

G

K

V

V

K

K

R

H

V

L

I

V

F

Y

A
|
A

S

S

N

S

H

S

V

V

T

Y

G

G

F

L

Y

N

G

R

Q

K

D

M

E

P

R

F

I

S

D

T

A

E

R

D

D

S

M

V

K

D

R

H

P

P

D

V

G

S

Y

L

Y
|
Y

G

A

L

A

S

T

K
|
K

S

K

Y

A

G

N

E

E

D

L

M

M

A

A

Q

H

F

T

Y

Y

F

S

D

H

R

L

Y

Y

G

G

V

I

E

P

T

T

V

T

S

G

I

L

R

R

I
x
F

G
x
F

S
x
T

I
x
V

F

Y

binding nicotinamide-adenine-dinucleotide: G8 (= G22), G11 (= G25), F12 (≠ G26), I13 (≠ V27), D32 (= D46), N33 (≠ L47), N35 (≠ S49), Y38 (≠ A52), K43 (≠ A57), D61 (= D67), L62 (= L68), L83 (= L87), A84 (≠ G88), A85 (≠ G89), A118 (= A125), Y145 (= Y152), K149 (= K156), F172 (≠ I179), F173 (≠ G180), T174 (≠ S181), V175 (≠ I182)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 181

7cgvA Full consensus l-threonine 3-dehydrogenase, fctdh-iiym (NAD+ bound form) (see paper)
27% identity, 59% coverage: 16:183/284 of query aligns to 1:170/310 of 7cgvA

query
sites
7cgvA

S

K

R

R

L

I

L

L

L

V

T

I

G
|
G

A

A

A

N

G
|
G

G
x
Q

V
x
I

G

G

K

S

Q

E

L

L

R

V

A

P

R

A

L

L

A

R

S

K

F

R

-

Y

-

G

A

A

E

D

V

N

V

V

R

I

V

A

A

S

D
|
D

L

-

A

-

S

-

A

-

M

-

A

-

V
x
I

D
x
R

P

P

A

P

A

N

A

L

H

Y

E

E

-

A

-

G

-

P

-

F

E

E

A

Y

L
|
L

G

-

C

-

D
|
D

L
x
V

A

L

D

D

R

K

A

E

A

A

V

L

D

A

A

E

M

L

V

V

A

K

G

K

C

Y

E

K

-

I

-

T

A

Q

I

I

I

Y

H

H

L

L

G
x
A

V

L

-
x
L

-

S

-

A

S

T

V

G

E

E

R

K

P

N

F

P

E

Q

E

K

I

A

L

W

E

D

A

L

N

N

I

M

K

D

G

G

V

L

F

L

H

N

I

V

Y

L

E

E

A

I

A

A

R

R

R

E

H

R

G

G

V

P

K

L

R

K

V

V

I

F

F

W

A
x
P

S

S

S
|
S

N

I

H

A

V

A

T

F

G

G

F

P

Y

N

G

T

Q

P

D

K

E

D

R

N

I

T

D

P

A

Q

R

D

D

T

M

I

K

M

R

R

P

P

D

T

G

T

Y

I

Y
|
Y

G

G

L

I

S

S

K
|
K

S

V

Y

A

G

G

E

E

D

L

M

L

A

C

Q

E

F

Y

Y

Y

F

H

D

T

R

K

Y

Y

G

G

V

V

E

D

T

V

V

R

S

S

I

V

R

R

I
x
Y

G

P

S

G

I
|
I

F

I

binding nicotinamide-adenine-dinucleotide: G7 (= G22), G10 (= G25), Q11 (≠ G26), I12 (≠ V27), D33 (= D46), I34 (≠ V54), R35 (≠ D55), L48 (= L64), D49 (= D67), V50 (≠ L68), A72 (≠ G88), A73 (≠ G89), L75 (vs. gap), P112 (≠ A125), S114 (= S127), Y139 (= Y152), K143 (= K156), Y166 (≠ I179), I169 (= I182)

3aw9A Structure of udp-galactose 4-epimerase mutant
28% identity, 58% coverage: 17:182/284 of query aligns to 2:161/304 of 3aw9A

query
sites
3aw9A

R

R

L

I

L

V

T

T

G
|
G

A

G

A

A

G
|
G

G
x
F

V
x
I

G

G

K

S

Q

H

L

L

R

V

A

D

R

K

L

L

A

V

S

E

F

L

A

G

E

Y

V

E

V

V

R

V

V

V

A

V

D
|
D

L
x
I

A

V

-

Q

-

R

-

D

-

T

-

G

S

S

A

A

M

E

A

L

A

H

V

V

D
x
R

P
x
D

A
x
L

A

K

A

D

H

Y

E

S

E

W

A

G

L

A

G

G

C

I

-

K

-

G

D

D

L

V

A

V

D

F

R

H

A
x
F

A
|
A

V
x
A

D

N

A
x
P

M

E

V
|
V

A

R

G

T

C

T

E

E

A

P

I

I

I

V

H

H

L

-

G

-

V

-

S

-

V

-

E

-

R

-

P

-

F

F

E

N

E
|
E

I

-

L

-

E

-

A

-

N

N

I

V

K

V

G

A

V

T

F

F

H

N

I

V

Y

L

E

E

A

W

A

A

R

R

R

Q

H

T

G

G

V

V

K

R

R

T

V

V

I

V

F

F

A
|
A

S
|
S

N
x
S

H
x
T

V

V

T

-

G

-

F

-

Y

Y

G

G

Q

D

D

A

E

D

R

V

I

I

D

P

A

T

R

P

D

E

M

E

K

E

-

P

-

Y

R

K

P

P

D

I

G

S

Y

V

Y
|
Y

G

G

L

A

S

A

K
|
K

S

A

Y

A

G

G

E

E

D

V

M

M

A

C

Q

A

F

T

Y

Y

F

A

D

R

R

L

Y

F

G

G

V

V

E

R

T

C

V

L

S

A

I

V

R

R

I
x
Y

G

A

S
x
N

I
x
V

active site: A105 (= A125), S107 (= S127), S108 (≠ N128), T109 (≠ H129), Y131 (= Y152), K135 (= K156)
binding galactose-uridine-5'-diphosphate: P69 (≠ A76), V71 (= V78), S107 (= S127), Y131 (= Y152), N160 (≠ S181)
binding nicotinamide-adenine-dinucleotide: G7 (= G22), G10 (= G25), F11 (≠ G26), I12 (≠ V27), D31 (= D46), I32 (≠ L47), R46 (≠ D55), D47 (≠ P56), L48 (≠ A57), F65 (≠ A72), A66 (= A73), A67 (≠ V74), E82 (= E98), A105 (= A125), S106 (= S126), Y131 (= Y152), K135 (= K156), Y158 (≠ I179), N160 (≠ S181), V161 (≠ I182)

Sites not aligning to the query:

active site: 166, 169
binding galactose-uridine-5'-diphosphate: 168, 170, 173, 188, 193, 195, 197, 234, 263, 266
binding nicotinamide-adenine-dinucleotide: 168

5z75A Artificial l-threonine 3-dehydrogenase designed by ancestral sequence reconstruction. (see paper)
24% identity, 57% coverage: 17:178/284 of query aligns to 4:166/306 of 5z75A

query
sites
5z75A

R

K

L

I

L

L

L

V

T

I

G
|
G

A

A

A
x
C

G
|
G

G
x
Q

V
x
I

G

G

K

T

Q

E

L

L

R

T

A

V

R

A

L

L

A

R

S

E

F

I

-

Y

-

G

A

N

E

E

V

N

V

V

R

V

V

A

A

S

D
|
D

L
x
I

A

R

S

E

A

P

M

N

A

E

V

S

D

G

P

P

A

F

A

E

A

K

H

-

E

-

A

-

L

-

G

-

C
x
L

D

D

L
x
V

A

M

D

D

R

K

A

E

A

R

V

L

D

E

A

E

M

I

V

V

A

E

G

K

-

H

-

K

C

I

E

T

A

Q

I

V

I

Y

H

H

L
|
L

G
x
A

G

A

V

I

-
x
L

-

S

-

A

S

T

V

G

E

E

R

K

P

N

F

P

E

L

E

F

I

A

L

W

E

D

A

L

N

N

I

M

K

N

G

S

V

L

F

L

H

N

I

V

Y

L

E

E

A

L

A

A

R

R

R

E

H

G

G

K

V

I

K

D

R

K

V

I

I

F

F

W

A
x
P

S

S

S
|
S

N

I

H

A

V

V

T

F

G

G

F

P

Y

T

G

T

Q

P

D

K

E

E

R

N

I

T

D

P

A

Q

R

H

D

T

M

V

K

M

R

D

P

P

D

S

G

T

Y

V

Y
|
Y

G

G

L

I

S

S

K
|
K

S

L

Y

A

G

G

E

E

D

R

M

W

A

C

Q

E

F

Y

Y

Y

F

H

D

E

R

K

Y

Y

G

G

V

V

E

D

T

V

V

R

S

S

I

I

R

R

binding nicotinamide-adenine-dinucleotide: G9 (= G22), C11 (≠ A24), G12 (= G25), Q13 (≠ G26), I14 (≠ V27), D35 (= D46), I36 (≠ L47), L49 (≠ C66), V51 (≠ L68), L72 (= L87), A73 (≠ G88), L76 (vs. gap), P113 (≠ A125), S115 (= S127), Y140 (= Y152), K144 (= K156)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 167, 168, 170, 176, 177

7eprB Partial consensus l-threonine 3-dehydrogenase (c-change) (see paper)
24% identity, 57% coverage: 17:178/284 of query aligns to 3:166/310 of 7eprB

query
sites
7eprB

R

K

L

I

L

L

L

I

T

V

G
|
G

A

A

A

C

G
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G

G
x
Q

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x
I

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G

K

S

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E

L

L

R

A

A

L

R

A

L

L

A

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F

R

A

Y

E

G

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N

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x
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x
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x
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N

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S

A

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H

E

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M

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L
x
D

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A

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L

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E

E

R

K

P

N

F

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Q

E

W

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L

W

E

N

A
x
L

N

N

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M

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G

S

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F

L

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N

I

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Y

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E

A

L

A

A

R

R

R

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K

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K

K

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R

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F

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x
P

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Y

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K

S

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N

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S

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R

R

binding nicotinamide-adenine-dinucleotide: G8 (= G22), G11 (= G25), Q12 (≠ G26), I13 (≠ V27), D34 (≠ A48), I35 (≠ S49), R36 (≠ A50), D50 (≠ L64), A51 (≠ G65), L72 (≠ I84), A73 (≠ I85), A74 (≠ H86), L90 (≠ A102), P113 (≠ A125), Y140 (= Y152), K144 (= K156)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 167, 170

3c1tB Binding of two substrate analogue molecules to dihydroflavonol 4- reductase alters the functional geometry of the catalytic site (see paper)
25% identity, 56% coverage: 18:177/284 of query aligns to 8:185/326 of 3c1tB

query
sites
3c1tB

L

V

L

C

L

V

T

T

G

G

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A

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x
S

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G

G
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F

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I

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L

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M

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R

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F

R

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E

Y

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R

-

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-

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x
R

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N

A

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x
K

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K

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H

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L

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V

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x
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M

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A

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F

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x
T

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S

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x
A

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G

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x
N

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I

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M

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K

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Y

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I

binding 3,5,7-trihydroxy-2-(3,4,5-trihydroxyphenyl)-4h-chromen-4-one: S125 (= S127), S125 (= S127), A126 (vs. gap), N130 (vs. gap), T156 (≠ P148), A157 (≠ D149), Y160 (= Y152), F161 (≠ G153)
binding nadp nicotinamide-adenine-dinucleotide phosphate: S14 (≠ A24), G15 (= G25), F16 (≠ G26), I17 (≠ V27), R37 (≠ A45), K44 (vs. gap), D64 (= D67), L65 (= L68), V84 (≠ L87), A85 (≠ G88), T86 (≠ G89), M88 (≠ S91), T123 (≠ A125), S124 (= S126), K164 (= K156)

Sites not aligning to the query:

5lc1A L-threonine dehydrogenase from trypanosoma brucei with NAD and the inhibitor pyruvate bound. (see paper)
26% identity, 60% coverage: 17:187/284 of query aligns to 3:178/320 of 5lc1A

query
sites
5lc1A

R

R

L

V

L

L

L

V

T

T

G
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G

A

A

A

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G
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G

G
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I

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G

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T

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L

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L

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L

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D

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D

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Y

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K

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Y

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E

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L

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W

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Y

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Y

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L

G

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I

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T

binding nicotinamide-adenine-dinucleotide: G8 (= G22), G11 (= G25), Q12 (≠ G26), I13 (≠ V27), D34 (= D46), V35 (≠ L47), D54 (= D67), C55 (≠ L68), L76 (= L87), P77 (≠ G88), A78 (≠ G89), M80 (≠ S91), P116 (≠ S126), T118 (≠ N128), Y143 (= Y152), K147 (= K156), L170 (≠ I179), I173 (= I182)
binding pyruvic acid: S81 (≠ V92), Y143 (= Y152)

Sites not aligning to the query:

binding pyruvic acid: 183, 185, 279

Query Sequence

>HSERO_RS23040 FitnessBrowser__HerbieS:HSERO_RS23040
MSQDQDVAAWQTARFSRLLLTGAAGGVGKQLRARLASFAEVVRVADLASAMAAVDPAAAH
EEALGCDLADRAAVDAMVAGCEAIIHLGGVSVERPFEEILEANIKGVFHIYEAARRHGVK
RVIFASSNHVTGFYGQDERIDARDMKRPDGYYGLSKSYGEDMAQFYFDRYGVETVSIRIG
SIFPEATNRRMLASWMSMDDFEQLLRRSLFIPDVGHTIVYGMSANAKTWWDNRYAAHLGY
APKDSSEIFRAKVEAQPMPAPDDPVLTLQGGAFTTAGPFDPLAD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory