SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing HSERO_RS23185 FitnessBrowser__HerbieS:HSERO_RS23185 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 7 hits to proteins with known functional sites (download)

Q29551 Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial; SCOT; 3-oxoacid CoA-transferase 1; Somatic-type succinyl-CoA:3-oxoacid CoA-transferase; SCOT-s; Succinate-coenzyme A transferase; Succinyl-CoA:3-oxoacid CoA transferase; EC 2.8.3.5 from Sus scrofa (Pig) (see 3 papers)
56% identity, 97% coverage: 5:214/216 of query aligns to 302:509/520 of Q29551

query
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Q29551

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E344 (= E47) active site, 5-glutamyl coenzyme A thioester intermediate

Sites not aligning to the query:

1:39 modified: transit peptide, Mitochondrion
67 C→A: Decreases KM for acetoacetyl-CoA 3-fold. No effect on KM for acetoacetate, succinyl-CoA and succinate.; C→S: Decreases KM for acetoacetate and acetoacetyl-CoA 2-fold. Increases KM for succinate 2-fold. Decreases KM for succinyl-CoA nearly 4-fold. Strongly reduces catalytic activity.
235 C→S: Similar specific activity to wild-type.

3k6mC Dynamic domains of succinyl-coa:3-ketoacid-coenzyme a transferase from pig heart. (see paper)
56% identity, 97% coverage: 5:214/216 of query aligns to 249:456/466 of 3k6mC

query
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3k6mC

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binding glycerol: I270 (= I26), E291 (= E47), A313 (= A69), V377 (= V133), A380 (≠ V136), T382 (vs. gap), C412 (≠ V171), N414 (= N173)

Sites not aligning to the query:

binding glycerol: 25, 52, 76, 99

1o9lA Succinate:coenzyme-a transferase (pig heart)
56% identity, 97% coverage: 5:214/216 of query aligns to 250:457/468 of 1o9lA

query
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1o9lA

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Sites not aligning to the query:

binding 2-(ethylmercuri-thio)-benzoic acid: 87, 94, 95, 96, 131, 132, 145

3oxoE Succinyl-coa:3-ketoacid coa transferase from pig heart covalently bound to coa (see paper)
57% identity, 94% coverage: 11:214/216 of query aligns to 251:452/462 of 3oxoE

query
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3oxoE

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binding coenzyme a: I266 (= I26), G267 (= G27), I268 (≠ L28), E287 (= E47), G343 (= G103), M345 (= M105), N355 (= N115), I358 (= I118), M366 (= M126), A369 (= A129), K389 (= K148)

8i3yD Crystal structure of asct from trypanosoma brucei in complex with succinyl-coa.
54% identity, 96% coverage: 5:212/216 of query aligns to 257:460/471 of 8i3yD

query
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8i3yD

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Sites not aligning to the query:

binding calcium ion: 201, 229

8i40A Crystal structure of asct from trypanosoma brucei in complex with coa.
54% identity, 96% coverage: 5:212/216 of query aligns to 253:456/467 of 8i40A

query
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8i40A

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V

T

V

P

D

G

G

G

R

L

L

K

V

L

L

I

K

E

E

L

I

A

A

S

E

G

D

V

T

T

T

K

V

E

D

E

Q

V

V

I

K

E

K

K

L

T

T

E

G

A

V

P

G

L

F

D

D

binding coenzyme a: I274 (= I26), I276 (≠ L28), E295 (= E47), G351 (= G103), L353 (≠ M105), N363 (= N115), G376 (= G128), K401 (= K157)

8i3yA Crystal structure of asct from trypanosoma brucei in complex with succinyl-coa.
54% identity, 95% coverage: 7:212/216 of query aligns to 249:450/459 of 8i3yA

query
sites
8i3yA

E

R

M

I

A

A

A

R

R

R

A

A

K

L

E

E

L

F

Q

A

D

N

G

G

F

M

Y

Y

V

V

N

N

L

L

G
|
G

I
|
I

G
|
G

L

I

P

P

T

T

L

E

V

S

A

S

N

N

H

Y

V

I

P

P

S

A

D

G

I

V

E

N

V

V

W

V

L

L

Q

Q

S

S

E
|
E

N

N

G

G

L

L

L

I

G

G

I

M

G

G

P

P

F

F

P

P

T

T

E

E

D

D

Q

K

V

V

D

D

P

A

D

D

L

W

I

I

N

N

A
|
A

G

G

K
|
K

Q

Q

T

T

V

I

T

S

T

H

I

L

P

A

G

G

S

S

S

A

F

L

F

F

S

D

S

S

A

A

D

T

S

S

F

F

G

A

M

M

I

I

R

R

G

G

K

H

I

M

N

D

I

L

A

T

I

M

L

L

G

G

A

A

M

L

Q

E

V

V

S

A

A

A

K

N

G

G

D

D

L

L

A

A

N
|
N

W

F

M

M

I

I

P

P

G

G

K

K

M

L

V

V

K

K

G

G

M

P

G
|
G

A
|
A

M

M

D

D

L

L

V

V

A

S

G

C

V

G

G

T

R

R

V

V

L

T

M

T

E

T

H

H

V

C

A
x
N

K
|
K

A

N

K

G

D

D

G

-

S

-

T

-

S

-

K

P

K
|
K

I

I

L

V

N

E

D

R

C

C

N

R

L

L

P

P

L

V

T

T

G

G

V

K

G

H

V

C

V

V

N

C

R

R

I

I

T

T

D

E

L

Y

G

A

V

V

I

F

D

D

V

V

T

V

P

D

G

G

G

R

L

L

K

V

L

L

I

K

E

E

L

I

A

A

S

E

G

D

V

T

T

T

K

V

E

D

E

Q

V

V

I

K

E

K

K

L

T

T

E

G

A

V

P

G

L

F

D

D

binding succinyl-coenzyme a: G267 (= G25), I268 (= I26), G269 (= G27), E289 (= E47), A311 (= A69), K313 (= K71), N357 (= N115), G369 (= G127), G370 (= G128), A371 (= A129), N389 (≠ A147), K390 (= K148), K395 (= K157)

Sites not aligning to the query:

Query Sequence

>HSERO_RS23185 FitnessBrowser__HerbieS:HSERO_RS23185
MAWTRDEMAARAAKELQDGFYVNLGIGLPTLVANHVPSDIEVWLQSENGLLGIGPFPTED
QVDPDLINAGKQTVTTIPGSSFFSSADSFGMIRGGKINIAILGAMQVSAKGDLANWMIPG
KMVKGMGGAMDLVAGVGRVVVLMEHVAKAKDGSTSKKILNDCNLPLTGVGVVNRIITDLG
VIDVTPGGLKLIELASGVTKEEVIEKTEAPLDVSEL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory