SitesBLAST

165:174 (vs. 129:138, 100% identical) binding
S174 (= S138) binding
WIT 198:200 (= WIT 162:164) binding
SR 222:223 (≠ AR 184:185) binding
G250 (= G212) to A: in IVA; unknown pathological significance
Y277 (≠ N239) binding
DLER 284:287 (≠ DFER 246:249) binding
E286 (= E248) active site, Proton acceptor; mutation to D: Residual isovaleryl-CoA dehydrogenase activity.; mutation to G: Loss of isovaleryl-CoA dehydrogenase activity. Does not affect isovaleryl-CoA dehydrogenase activity; when associated with 407-E.; mutation to Q: Loss of isovaleryl-CoA dehydrogenase activity.
A291 (≠ S253) to V: in IVA; unknown pathological significance; dbSNP:rs886042098
R312 (= R274) binding
Q323 (= Q285) binding
I379 (= I347) to T: in IVA; unknown pathological significance
QCFGG 380:384 (≠ QSLGG 348:352) binding
R398 (= R366) to Q: in IVA; unknown pathological significance; dbSNP:rs1477527791
Y403 (= Y371) to N: in IVA; unknown pathological significance
A407 (= A375) mutation to E: Does not affect isovaleryl-CoA dehydrogenase activity; when associated with 286-D.
AG 407:408 (= AG 375:376) binding
TSE 409:411 (= TSE 377:379) binding

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion

5lnxD Crystal structure of mmgc, an acyl-coa dehydrogenase from bacillus subtilis.
42% identity, 96% coverage: 11:390/394 of query aligns to 2:373/374 of 5lnxD

query
sites
5lnxD

H

H

G

V

E

Q

D

E

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Q

A

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M

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D

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F

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E

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G

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E

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Y

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A

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G

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A

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N

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x
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active site: L122 (≠ M131), T123 (≠ S132), G239 (≠ E248), E358 (≠ A375), K370 (≠ R387)
binding flavin-adenine dinucleotide: F120 (≠ L129), L122 (≠ M131), T123 (≠ S132), G128 (= G137), S129 (= S138), F153 (≠ W162), I154 (= I163), T155 (= T164), R265 (= R274), Q267 (= Q276), F268 (= F277), I272 (= I281), N275 (≠ F284), I278 (≠ M287), Q331 (= Q348), I332 (≠ S349), G334 (= G351), G335 (= G352), Y336 (≠ N353), Y357 (≠ G374), T360 (= T377), E362 (= E379)

5ol2C The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
42% identity, 95% coverage: 18:390/394 of query aligns to 11:377/378 of 5ol2C

query
sites
5ol2C

L

L

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K

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E

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Y

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Y

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L

active site: L124 (≠ M131), T125 (≠ S132), G241 (≠ E248), G374 (≠ R387)
binding calcium ion: E29 (= E36), E33 (≠ S40)
binding coenzyme a persulfide: T125 (≠ S132), T131 (≠ S138), S134 (≠ V141), F231 (≠ V238), M235 (= M242), L238 (= L245), R242 (= R249), E362 (≠ A375), G363 (= G376), V367 (≠ I380)
binding flavin-adenine dinucleotide: F122 (≠ L129), L124 (≠ M131), T125 (≠ S132), P127 (= P134), G130 (= G137), T131 (≠ S138), F155 (≠ W162), F155 (≠ W162), I156 (= I163), T157 (= T164), T208 (= T215), R267 (= R274), Q269 (= Q276), F270 (= F277), L274 (≠ I281), F277 (= F284), T280 (≠ M287), Q335 (= Q348), L336 (≠ S349), H337 (≠ L350), G338 (= G351), G339 (= G352), Y340 (≠ N353), Y340 (≠ N353), T343 (≠ I356), D345 (≠ E358), Y346 (= Y359), I357 (≠ L370), I360 (= I373), Y361 (≠ G374), G363 (= G376), T364 (= T377), E366 (= E379)

P15651 Short-chain specific acyl-CoA dehydrogenase, mitochondrial; SCAD; Butyryl-CoA dehydrogenase; EC 1.3.8.1 from Rattus norvegicus (Rat) (see 2 papers)
40% identity, 99% coverage: 2:390/394 of query aligns to 25:407/412 of P15651

query
sites
P15651

I

L

H

H

L

T

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S

F

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A

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x
F

A
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M
x
L

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G

A
x
N

G
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G

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S

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V

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A

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M

L

L

V

I

I

G

A

R

G

E

H

L

L

F

L

152:161 (vs. 129:138, 60% identical) binding
S161 (= S138) binding
WIT 185:187 (= WIT 162:164) binding
DMGR 269:272 (≠ DFER 246:249) binding
R297 (= R274) binding
QILGG 365:369 (≠ QSLGG 348:352) binding
E392 (≠ A375) active site, Proton acceptor
TSE 394:396 (= TSE 377:379) binding

Sites not aligning to the query:

1:24 modified: transit peptide, Mitochondrion

1jqiA Crystal structure of rat short chain acyl-coa dehydrogenase complexed with acetoacetyl-coa (see paper)
40% identity, 97% coverage: 8:390/394 of query aligns to 4:380/384 of 1jqiA

query
sites
1jqiA

S

S

F

V

D

E

H

L

G

P

E

E

D

T

I

H

A

Q

A

M

L

L

R

R

E

Q

A

T

V

C

A

R

A

D

F

F

A

A

H

E

S

K

E

E

I

L

A

V

P

P

R

I

A

A

E

Q

I

L

D

D

R

K

S

E

D

H

Q

L

F

F

P

P

M

T

D

S

L

Q

W

V

K

K

L

M

G

G

D

E

L

L

G

G

V

L

L

L

G

A

I

M

T

D

V

V

S

P

E

E

Y

L

G

S

G

G

A

A

G

G

L

L

G

D

Y

Y

L

L

A

A

H

Y

I

S

I

I

A

A

M

L

E

E

I

I

S

S

R

R

A

G

S

C

A

A

S

S

V

T

G

G

L

V

S

I

Y

M

G

S

A

V

H

N

S

N

N

S

L
|
L

C

Y

V

L

N

G

Q

P

I

I

K

L

R

K

N

F

G

G

N

S

E

S

E

Q

Q

Q

K

K

R

Q

K

Q

Y

W

L

I

P

T

R

P

L

F

I

T

S

N

G

G

D

D

F

K

I

I

G

G

A

C

L
x
F

A
|
A

M
x
L

S
|
S

E

E

P

P

N

G

A

N

G
|
G

S
|
S

D

D

V
x
A

V

G

S

A

M

A

K

S

L

T

R

T

A

A

D

R

K

E

G

G

D

D

R

S

Y

W

V

V

L

L

N

N

G

G

S

T

K

K

M

A

W
|
W

I
|
I

T
|
T

N

N

G

S

P

W

D

E

A

A

D

S

V

A

L

T

V

V

Y

F

A

A

K

S

T

T

D

D

L

R

E

S

A

R

G

Q

A

N

R
x
K

G

G

M

I

T

S

A

A

F

F

L

L

V

V

E

P

K

M

G

P

Y

T

K

P

G

G

F

L

S

T

V

L

A

G

Q

K

K

K

L

E

D

D

K
|
K

L

L

G

G

M

I

R

R

G

A

S

S

H

S

T
|
T

G

A

E

N

L

L

V

I

F

F

Q

E

D

D

C

C

E

R

V

I

P

P

E

K

E

E

N

N

V

L

L

L

G

G

G

E

V

P

G

G

R

M

G

G

V
x
F

N
x
K

V

I

L

A

M
|
M

S
x
Q

G

T

L
|
L

D
|
D

F

M

E

G

R
|
R

S

I

V

G

L

I

S

A

G

S

G

Q

P

A

L

L

G

G

I

I

M

A

Q

Q

A

A

C

S

M

L

D

D

V

C

V

A

V

V

P

K

Y

Y

V

A

H

E

D

N

R
|
R

K

H

Q

A

F
|
F

G

G

Q

A

A

P

I
x
L

G

T

E

K

F
x
L

Q

Q

L

N

M
x
I

Q

Q

G

F

K

K

L

L

A

A

D

D

M

M

Y

A

S

L

T

A

M

L

M

E

A

S

C

A

K

R

A

L

Y

L

V

T

Y

W

A

R

V

A

G

-

Q

-

A

-

C

-

D

-

R

-

A

A

D

M

S

L

A

K

D

D

K

N

V

K

R

K

A

P

L

F

R
x
T

K

K

D

E

A

S

A

A

G

M

A

A

I

K

L

L

Y

A

S

A

A

S

E

E

K

A

A

A

T

T

W

A

M

I

A

S

G

H

E

Q

A

A

I

I

Q
|
Q

S
x
I

L

L

G
|
G

N

M

G

G

Y
|
Y

I

V

N

T

E

E

Y

M

P

P

V

A

G

E

R

R

L

Y

W

Y

R

R

D

D

A

A

K

R

L
x
I

Y

T

E

E

I

I

G
x
Y

A
x
E

G
|
G

T
|
T

S

S

E
|
E

I
|
I

R

Q

R

R

M
x
L

L

V

I

I

G

A

R
x
G

E

H

L

L

F

L

active site: G377 (≠ R387)
binding acetoacetyl-coenzyme a: L95 (= L99), F125 (≠ L129), L127 (≠ M131), G133 (= G137), S134 (= S138), A136 (≠ V140), K181 (≠ R185), F234 (≠ V238), K235 (≠ N239), M238 (= M242), Q239 (≠ S243), L241 (= L245), D242 (= D246), R245 (= R249), T315 (≠ R325), Y364 (≠ G374), E365 (≠ A375), G366 (= G376), I370 (= I380)
binding flavin-adenine dinucleotide: F125 (≠ L129), A126 (= A130), L127 (≠ M131), S128 (= S132), G133 (= G137), S134 (= S138), W158 (= W162), I159 (= I163), T160 (= T164), K203 (= K207), T211 (= T215), R270 (= R274), F273 (= F277), L277 (≠ I281), L280 (≠ F284), I283 (≠ M287), Q338 (= Q348), I339 (≠ S349), G341 (= G351), G342 (= G352), Y345 (= Y355), I360 (≠ L370), Y364 (≠ G374), T367 (= T377), E369 (= E379), I370 (= I380), L373 (≠ M383)

P16219 Short-chain specific acyl-CoA dehydrogenase, mitochondrial; SCAD; Butyryl-CoA dehydrogenase; EC 1.3.8.1 from Homo sapiens (Human) (see 3 papers)
42% identity, 93% coverage: 25:390/394 of query aligns to 48:407/412 of P16219

query
sites
P16219

F

F

A

A

H

E

S

K

E

E

I

L

A

F

P

P

R

I

A

A

E

Q

I

V

D

D

R

K

S

E

D

H

Q

L

F

F

P

P

M

A

D

A

L

Q

W

V

K

K

L

M

G

G

D

G

L

L

G

G

V

L

L

L

G

A

I

M

T

D

V

V

S

P

E

E

Y

L

G

G

G
|
G

A

A

G

G

L

L

G

D

Y

Y

L

L

A

A

H

Y

I

A

I

I

A

A

M

M

E

E

E
|
E

I

I

S

S

R

R

A

G

S

C

A

A

S

S

V

T

G

G

L

V

S

I

Y

M

G

S

A

V

H

N

S

N

N

S

L

L

C

Y

V

L

N

G

Q

P

I

I

K

L

R

K

N

F

G

G

N

S

E

K

E

E

Q

Q

K

K

R

Q

K

A

Y

W

L

V

P

T

R

P

L

F

I

T

S

S

G

G

D

D

F

K

I

I

G

G

A

C

L
x
F

A
|
A

M
x
L

S
|
S

E
|
E

P
|
P

N
x
G

A
x
N

G
|
G

S
|
S

D

D

V

A

V

G

S

A

M

A

K

S

L

T

R

T

A

A

D
x
R

K

A

K

E

G

G

D

D

R

S

Y

W

V

V

L

L

N

N

G

G

S

T

K

K

M

A

W
|
W

I
|
I

T
|
T

N

N

G

A

P

W

D

E

A
|
A

D

S

V

A

L

A

V

V

Y

F

A

A

K

S

T

T

D

D

L

R

E

A

A

L

G

Q

A

N

R

K

G
|
G

M

I

T

S

A

A

F

F

L

L

V

V

E

P

K

M

G

P

Y

T

K

P

G

G

F

L

S

T

V

L

A

G

Q

K

K

K

L

E

D

D

K

K

L

L

G

G

M

I

R

R

G

G

S

S

H

S

T

T

G

A

E

N

L

L

V

I

F

F

Q

E

D

D

C

C

E

R

V

I

P

P

E

K

E

D

N

S

V

I

L

L

G

G

G

E

V

P

G

G

R

M

G

G

V

F

N

K

V

I

L

A

M

M

S

Q

G

T

L

L

D

D

F

M

E

G

R

R

S

I

V

G

L

I

S

A

G

S

G

Q

P

A

L

L

G

G

I

I

M

A

Q

Q

A

T

C

A

M

L

D

D

V

C

V

A

V

V

P

N

Y

Y

V

A

H

E

D

N

R
|
R

K

M

Q

A

F

F

G

G

Q

A

A

P

I

L

G

T

E

K

F

L

Q
|
Q

L

V

M

I

Q

Q

G

F

K

K

L

L

A

A

D

D

M

M

Y

A

S

L

T

A

M

L

M

E

A

S

C

A

K
x
R

A

L

Y

L

V

T

Y

W

A

R

V

A

G

-

Q

-

A

-

C

-

D

-

R

-

A

A

D

M

S

L

A

K

D

D

K

N

V

K

R

K

A

P

L

F

R

I

K

K

D

E

A

A

G

M

A

A

I

K

L

L

Y

A

S

A

A
x
S

E

E

K

A

A

A

T

T

W

A

M

I

A

S

G

H

E

Q

A

A

I

I

Q
|
Q

S
x
I

L
|
L

G
|
G

N

M

G

G

Y

Y

I

V

N

T

E

E

Y

M

P

P

V

A

G

E

R
|
R

L

H

W

Y

R

R

D

D

A

A

K

R

L

I

Y

T

E

E

I

I

G

Y

A

E

G

G

T
|
T

S
|
S

E
|
E

I

I

R

Q

R

R

M

L

L

V

I

I

G

A

R

G

E

H

L

L

F

L

G90 (= G67) to S: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs121908005
E104 (= E81) natural variant: Missing (in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs387906308)
152:161 (vs. 129:138, 60% identical) binding in other chain
R171 (≠ D148) to W: 69% of wild-type acyl-CoA dehydrogenase activity; confers susceptibility to ethylmalonicaciduria; dbSNP:rs1800556
WIT 185:187 (= WIT 162:164) binding in other chain
A192 (= A169) to V: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28940874
G209 (= G186) to S: 86% of wild-type acyl-CoA dehydrogenase activity; confers susceptibility to ethylmalonicaciduria; dbSNP:rs1799958
R297 (= R274) binding
Q308 (= Q285) binding in other chain
R325 (≠ K302) to W: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs121908006
S353 (≠ A336) to L: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28941773
QILGG 365:369 (≠ QSLGG 348:352) binding
R380 (= R363) to W: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28940875
TSE 394:396 (= TSE 377:379) binding in other chain

Sites not aligning to the query:

1:24 modified: transit peptide, Mitochondrion

4l1fA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
37% identity, 98% coverage: 7:392/394 of query aligns to 1:380/380 of 4l1fA

query
sites
4l1fA

L

M

S

D

F

F

D

N

H
x
L

G
x
T

E

E

D

D

I

Q

A
x
Q

A

M

L

I

R

K

E

D

A

M

V

A

A

A

A

E

F

F

A

A

H

E

S

K

E

F

I

L

A

A

P

P

R

T

A

V

A

E

E

E

I

R

D

D

R

K

S

A

D

H

Q

I

F

W

P

D

M

R

D

K

L

L

W

I

K

D

K

K

L

M

G

G

D

E

L

A

G

G

V

F

L

C

G

G

I

I

T

C

V

F

S

P

E

E

Y

Y

G

G

A

M

G

G

L

L

G

D

Y

V

L

L

A

S

H

Y

I

I

I

L

A

A

M

V

E

E

I

L

S

S

R

K

A

V

S

D

A

D

S

G

V

T

G

G

L

I

S

T

Y

L

G

S

A

A

H

N

S

V

N

S

L

L

C

C

V

A

N

T

Q

P

I

I

K

Y

R

M

N

F

G

G

N

T

E

E

Q

Q

K

K

R

Q

K

K

Y

Y

L

L

P

A

R

P

L

I

I

A

S

E

G

G

D

T

F

H

I

V

G

G

A

A

L
x
F

A

G

M
x
L

S
x
T

E

E

P

P

N

S

A

A

G
|
G

S
x
T

D

D

V
x
A

V

S

S

A

M

Q

K

Q

L

T

R

T

A

A

D

V

K

L

K

K

G

G

D

D

R

K

Y

Y

V

I

L

L

N

N

G

G

S

S

K

K

M

I

W
x
F

I
|
I

T
|
T

N

N

G

G

P

K

D

E

A

A

D

D

V

T

L

Y

V

V

Y

F

A

A

K

M

T

T

D

D

L

K

E

S

A

Q

G

G

A
x
V

R
x
H

G

G

M

I

T

S

A

A

F

F

L

I

V

L

E

E

K

K

G

G

Y

M

K

P

G

G

F

F

S

R

V

F

A

G

Q

K

K

I

L

E

D

D

K

K

L

M

G

G

M

G

R

H

G

T

S

S

H

I

T
|
T

G

A

E

E

L

L

V

I

F

F

Q

E

D

D

C

C

E

E

V

V

P

P

E

K

E

E

N

N

V

L

L

L

G

G

G

K

V

E

G

G

R

E

G

G

V
x
F

N

K

V

I

L

A

M
|
M

S
x
E

G

T

L
|
L

D
|
D

F

G

E
x
G

R
|
R

S

I

V

G

L

V

S

A

G

A

G

Q

P

A

L

L

G

G

I

I

M

A

Q

E

A

G

C

A

M

L

D

A

V

A

V

V

P

K

Y

Y

V

S

H

K

D

E

R
|
R

K

E

Q
|
Q

F
|
F

G

G

Q

R

A

S

I
|
I

G

S

E

K

F
|
F

Q

Q

L

A

M
x
L

Q

Q

G

F

K

M

L

M

A

A

D

D

M

M

Y

A

S

T

T

K

M

I

M

E

A

A

C

A

K

R

A

Y

Y

L

V

V

Y

Y

A

H

V

A

G

A

Q

M

A

L

C

K

D

N

R

E

A

G

D

K

S

P

A

Y

D
x
S

K

E

V

A

R

A

A

A

L

M

R

A

K

K

D

-

A

-

G

-

A

-

I

-

L

C

Y

F

S

A

A

S

E

D

K

V

A

A

T

M

W

E

M

V

A

T

G

T

E

D

A

A

I

V

Q
|
Q

S
x
I

L

F

G
|
G

N
x
Y

G

G

Y

Y

I

T

N

V

E

D

Y

Y

P

P

V

A

G

E

R

R

L

Y

W

M

R

R

D

N

A

A

K

K

L
x
I

Y

T

E

Q

I

I

G
x
Y

A
x
E

G
|
G

T
|
T

S

N

E
x
Q

I
x
V

R

M

R

R

M
x
I

L

V

I

T

G

S

R
|
R

E

A

L

L

F

L

A

R

D

D

active site: L125 (≠ M131), T126 (≠ S132), G242 (≠ E248), E363 (≠ A375), R375 (= R387)
binding coenzyme a persulfide: F123 (≠ L129), T132 (≠ S138), A134 (≠ V140), V178 (≠ A184), H179 (≠ R185), F232 (≠ V238), M236 (= M242), E237 (≠ S243), L239 (= L245), D240 (= D246), R243 (= R249), S313 (≠ D319), Y362 (≠ G374), E363 (≠ A375), G364 (= G376), V368 (≠ I380), R375 (= R387)
binding flavin-adenine dinucleotide: F123 (≠ L129), L125 (≠ M131), T126 (≠ S132), G131 (= G137), T132 (≠ S138), F156 (≠ W162), I157 (= I163), T158 (= T164), T209 (= T215), R268 (= R274), Q270 (= Q276), F271 (= F277), I275 (= I281), F278 (= F284), L281 (≠ M287), Q336 (= Q348), I337 (≠ S349), G339 (= G351), G340 (= G352), Y341 (≠ N353), I358 (≠ L370), Y362 (≠ G374), T365 (= T377), Q367 (≠ E379), I371 (≠ M383)
binding 1,3-propandiol: L5 (≠ H11), T6 (≠ G12), Q10 (≠ A16)

2vigB Crystal structure of human short-chain acyl coa dehydrogenase
41% identity, 93% coverage: 25:390/394 of query aligns to 15:367/371 of 2vigB

query
sites
2vigB

F

F

A

A

H

E

S

K

E

E

I

L

A

F

P

P

R

I

A

A

E

Q

I

V

D

D

R

K

S

E

D

H

Q

L

F

F

P

P

M

A

D

A

L

Q

W

V

K

K

L

M

G

G

D

G

L

L

G

G

V

L

L

L

G

A

I

M

T

D

V

V

S

P

E

E

Y

L

G

G

A

A

G

G

L

L

G

D

Y

Y

L

L

A

A

H

Y

I

A

I

I

A

A

M

M

E

E

I

I

S

S

R

R

A

G

S

C

A

A

S

S

V

T

G

G

L

V

S

I

Y

M

G

S

A

V

H

N

S

N

N

S

L

L

C

Y

V

L

N

G

Q

P

I

I

K

L

R

K

N

F

G

G

N

S

E

K

E

E

Q

Q

K

K

R

Q

K

A

Y

W

L

V

P

T

R

P

L

F

I

T

S

S

G

G

D

D

F

K

I

I

G

G

A

C

L
x
F

A
|
A

M
x
L

S
|
S

E

E

P

P

N

G

A

N

G
|
G

S
|
S

D

D

V

A

V

G

S

A

M

A

K

S

L

T

R

T

A

A

D

R

K

A

K

E

G

G

D

D

R

S

Y

W

V

V

L

L

N

N

G

G

S

T

K

K

M
x
A

W
|
W

I
|
I

T
|
T

N

N

G

A

P

W

D

E

A

A

D

S

V

A

L

A

V

V

Y

F

A

A

K

S

T

T

D

S

L

I

E

-

A

-

G

-

A

-

R

-

G

-

M

-

T

S

A

A

F

F

L

L

V

V

E

P

K

M

G

P

Y

T

K

P

G

G

F

L

S

T

V

L

A

G

Q

K

K

K

L

E

D

D

K
|
K

L

L

G

G

M

I

R

R

G

G

S

S

H

S

T
|
T

G

A

E

N

L

L

V

I

F

F

Q

E

D

D

C

C

E

R

V

I

P

P

E

K

E

D

N

S

V

I

L

L

G

G

G

E

V

P

G

G

R

M

G

G

V
x
F

N
x
K

V

I

L

A

M
|
M

S
x
Q

G

T

L
|
L

D
|
D

F

M

E
x
G

R
|
R

S

I

V

G

L

I

S

A

G

S

G

Q

P

A

L

L

G

G

I

I

M

A

Q

Q

A

T

C

A

M

L

D

D

V

C

V

A

V

V

P

N

Y

Y

V

A

H

E

D

N

R
|
R

K

M

Q

A

F
|
F

G

G

Q

A

A

P

I
x
L

G

T

E

K

F
x
L

Q

Q

L

V

M
x
I

Q

Q

G

F

K

K

L

L

A

A

D

D

M

M

Y

A

S

L

T

A

M

L

M

E

A

S

C

A

K

R

A

L

Y

L

V

T

Y

W

A

R

V

A

G

-

Q

-

A

-

C

-

D

-

R

-

A

A

D

M

S

L

A

K

D

D

K

N

V

K

R

K

A

P

L

F

R
x
I

K

K

D

E

A

A

G

M

A

A

I

K

L

L

Y

A

S

A

A

S

E

E

K

A

A

A

T

T

W

A

M

I

A

S

G

H

E

Q

A

A

I

I

Q
|
Q

S
x
I

L

L

G
|
G

N

M

G

G

Y

Y

I

V

N

T

E

E

Y

M

P

P

V

A

G

E

R

R

L

H

W

Y

R

R

D

D

A

A

K

R

L
x
I

Y

T

E

E

I
|
I

G
x
Y

A
x
E

G
|
G

T
|
T

S

S

E
|
E

I
|
I

R

Q

R

R

M
x
L

L

V

I

I

G

A

R
x
G

E

H

L

L

F

L

active site: L121 (≠ M131), S122 (= S132), G231 (≠ E248), E352 (≠ A375), G364 (≠ R387)
binding coenzyme a persulfide: F119 (≠ L129), G127 (= G137), S128 (= S138), F221 (≠ V238), K222 (≠ N239), M225 (= M242), Q226 (≠ S243), L228 (= L245), D229 (= D246), R232 (= R249), I302 (≠ R325), E352 (≠ A375), G353 (= G376), I357 (= I380)
binding flavin-adenine dinucleotide: F119 (≠ L129), A120 (= A130), L121 (≠ M131), S122 (= S132), G127 (= G137), S128 (= S138), A151 (≠ M161), W152 (= W162), I153 (= I163), T154 (= T164), K190 (= K207), T198 (= T215), R257 (= R274), F260 (= F277), L264 (≠ I281), L267 (≠ F284), I270 (≠ M287), Q325 (= Q348), I326 (≠ S349), G328 (= G351), G329 (= G352), I347 (≠ L370), I350 (= I373), Y351 (≠ G374), T354 (= T377), E356 (= E379), I357 (= I380), L360 (≠ M383)

3pfdC Crystal structure of an acyl-coa dehydrogenase from mycobacterium thermoresistibile bound to reduced flavin adenine dinucleotide solved by combined iodide ion sad mr (see paper)
38% identity, 95% coverage: 17:390/394 of query aligns to 3:368/369 of 3pfdC

query
sites
3pfdC

A

A

L

L

R

R

E

E

A

A

V

I

A

R

A

A

F

L

A

A

H

E

S

K

E

E

I

I

A

A

P

P

R

Y

A

A

E

E

I

V

D

D

R

E

S

K

D

A

Q

R

F

F

P

P

M

E

D

E

L

A

W

L

K

A

L

L

G

N

D

S

L

S

G

G

V

F

L

S

G

A

I

I

T

H

V

V

S

P

E

E

Y

Y

G

G

A

Q

G

G

L

A

G

D

Y

S

L

V

A

A

H

T

I

C

I

I

A

V

M

I

E

E

I

V

S

A

R

R

A

V

S

D

A

C

S

S

V

A

G

S

L

L

S

I

Y

P

G

A

A

V

H

N

S

K

N

L

L

G

C

T

V

M

N

G

Q

L

I

I

K

L

R

R

N

-

G

G

N

S

E

E

Q

L

K

K

R

K

K

Q

Y

V

L

L

P

P

R

A

L

V

I

A

S

S

G

G

D

E

F

A

I

M

G

A

A

S

L
x
Y

A
|
A

M
x
L

S
|
S

E

E

P

R

N

E

A

A

G
|
G

S
|
S

D

D

V

A

S

S

M

M

K

R

L

T

R

R

A

A

D

V

K

A

K

D

G

G

D

D

R

D

Y

W

V

I

L

L

N

N

G

G

S

S

K

K

M

C

W
|
W

I
|
I

T
|
T

N

N

G

G

P

G

D

K

A

S

D

T

V

W

L

Y