SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing IAI46_00835 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

P0A890 Sulfur carrier protein TusA; Sulfur mediator TusA; Sulfur transfer protein TusA; tRNA 2-thiouridine synthesizing protein A from Escherichia coli (strain K12) (see 2 papers)
83% identity, 96% coverage: 1:80/83 of query aligns to 1:80/81 of P0A890

query
sites
P0A890

M

M

T

T

D

D

L

L

F

F

A

S

Q

S

A

P

D

D

Q

H

T

T

L

L

D

D

A

A

L

L

G

G

L

L

R

R

C
|
C

P

P

E
|
E

P

P

V

V

M

M

V

V

R

R

K

K

T

T

V

V

R

R

H

N

M

M

D

Q

N

P

G

G

E

E

T

T

L

L

I

I

A

A

D

D

P

P

A

A

T

T

R

R

D

D

I

I

P

P

G

G

F

F

C
|
C

R

T

F

F

M

M

E

E

H

H

T

E

L

L

V

V

A

A

Q

K

E

E

T

T

E

D

K

G

T

L

P

P

Y

Y

R

R

Y

Y

L

L

L

I

R

R

K

K

G

G

C19 (= C19) active site, Cysteine persulfide intermediate; mutation to S: Unable to restore mnm5s2U formation in a tusA-deficient mutant. Unable to accept sulfur from IscS.
E21 (= E21) mutation to K: In sirA1; affects the stability of sigma-S during the logarithmic growth phase.
C56 (= C56) mutation to S: Able to restore mnm5s2U formation in a tusA-deficient mutant.

P0A892 Sulfur carrier protein TusA; Sulfur mediator TusA; Sulfur transfer protein TusA; tRNA 2-thiouridine synthesizing protein A from Escherichia coli O157:H7 (see paper)
83% identity, 96% coverage: 1:80/83 of query aligns to 1:80/81 of P0A892

query
sites
P0A892

M

M

T

T

D

D

L

L

F

F

A

S

Q

S

A

P

D

D

Q

H

T

T

L

L

D

D

A

A

L

L

G

G

L

L

R

R

C

C

P

P

E
|
E

P

P

V

V

M
|
M

M

M

V

V

R
|
R

K
|
K

T
|
T

V
|
V

R
|
R

H
x
N

M
|
M

D
x
Q

N
x
P

G

G

E

E

T

T

L

L

I

I

A

A

D

D

D
|
D

P

P

A

A

T

T

R
|
R

D
|
D

I

I

P

P

G

G

F

F

C

C

R

T

F
|
F

M

M

E

E

H

H

T

E

L

L

V

V

A

A

Q

K

E

E

T

T

E

D

K

G

T

L

P

P

Y

Y

R

R

Y

Y

L

L

L

I

R

R

K

K

G

G

E21 (= E21) mutation to A: Decreased binding of IscS.
M24 (= M24) mutation to R: No binding of IscS, 50% modified tRNA produced.
R27 (= R27) mutation R->D,E: No binding of IscS, 19% modified tRNA produced (for R27E).
27:35 (vs. 27:35, 67% identical) mutation to DKTVRNMQS: No modified tRNA produced.
R31 (= R31) mutation to A: No binding of IscS, 30% modified tRNA produced.
D45 (= D45) mutation to A: Binds IscS, 56% modified tRNA produced.
R50 (= R50) mutation to A: Binds IscS, 67% modified tRNA produced.
D51 (= D51) mutation to A: Binds IscS, 67% modified tRNA produced.
F58 (= F58) mutation to A: No binding of IscS, 67% modified tRNA produced.

D3RPC0 Sulfur carrier protein TusA from Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / NCIMB 10441 / D) (Chromatium vinosum) (see paper)
35% identity, 89% coverage: 6:79/83 of query aligns to 2:75/76 of D3RPC0

query
sites
D3RPC0

A

A

Q

D

A

F

D

D

Q

Q

T

E

L

L

D

D

A

A

L

S

G

G

L

L

R

N

C
|
C

P

P

E

L

P

P

V

I

M

L

M

R

V

A

R

K

K

K

T

T

V

L

R

N

H

A

M

M

D

S

N

S

G

G

E

Q

T

V

L

L

L

H

I

V

I

I

A

A

D

T

D

D

P

P

A

G

T

S

T

V

R

K

D

D

I

F

P

D

G

A

F

F

C

A

R

K

F

Q

M

T

E

G

H

N

T

E

L

L

V

M

A

E

Q

S

E

K

T

E

E

E

K

G

T

G

P

K

Y

F

R

H

Y

F

L

L

L

I

R

K

K

K

C15 (= C19) active site, Cysteine persulfide intermediate; mutation to S: Loss of sulfur binding in the presence of sulfide. Loss of interaction with DsrEFH proteins.

Query Sequence

>IAI46_00835
MTDLFAQADQTLDALGLRCPEPVMMVRKTVRHMDNGETLLIIADDPATTRDIPGFCRFME
HTLVAQETEKTPYRYLLRKGVER

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory