SitesBLAST
Comparing N515DRAFT_0013 FitnessBrowser__Dyella79:N515DRAFT_0013 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
32% identity, 94% coverage: 2:409/433 of query aligns to 9:423/426 of 6xwnB
5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
32% identity, 94% coverage: 2:409/433 of query aligns to 9:423/427 of 5e9sA
- binding aspartic acid: R274 (≠ S267), S275 (= S268), S276 (= S269), T313 (= T305), G353 (= G345), V354 (= V346), A357 (= A349), G358 (= G350), D394 (≠ S380), R397 (= R383), T398 (≠ A384)
- binding decyl-beta-d-maltopyranoside: L194 (≠ R187), G198 (≠ M191), Y202 (≠ L195)
- binding sodium ion: Y87 (≠ F80), T90 (≠ V83), S91 (= S84), S276 (= S269), G305 (= G297), A306 (≠ Y298), T307 (≠ S299), N309 (= N301), N309 (= N301), M310 (≠ L302), D311 (= D303), S348 (= S340), I349 (≠ K341), G350 (= G342), T351 (≠ A343), N401 (= N387), V402 (≠ I388), D405 (≠ N391)
6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
32% identity, 94% coverage: 2:409/433 of query aligns to 6:420/424 of 6zl4A
- binding decyl-beta-d-maltopyranoside: L191 (≠ R187), G195 (≠ M191), R282 (≠ K279)
- binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ S267), S272 (= S268), S273 (= S269), M307 (≠ L302), T310 (= T305), G353 (= G348), A354 (= A349), R394 (= R383), T395 (≠ A384)
Sites not aligning to the query:
6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
32% identity, 94% coverage: 2:409/433 of query aligns to 7:421/425 of 6zgbA
6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
32% identity, 94% coverage: 2:409/433 of query aligns to 2:412/416 of 6r7rA
- binding d-aspartic acid: R263 (≠ S267), S265 (= S269), M299 (≠ L302), T302 (= T305), T340 (≠ A343), G342 (= G345), V343 (= V346), G347 (= G350), D383 (≠ S380), R386 (= R383), T387 (≠ A384), N390 (= N387)
- binding decyl-beta-d-maltopyranoside: H23 (= H24), V212 (≠ L216), A216 (≠ L220)
2nwwA Crystal structure of gltph in complex with tboa (see paper)
32% identity, 90% coverage: 12:402/433 of query aligns to 12:407/407 of 2nwwA
O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
30% identity, 94% coverage: 2:407/433 of query aligns to 11:421/425 of O59010
- S65 (≠ T55) mutation to V: Strongly decreased chloride conductance.
- R276 (≠ S267) mutation to S: Increased rate of aspartate transport; when associated with R-395.
- RSS 276:278 (≠ SSS 267:269) binding
- M311 (≠ L302) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
- T314 (= T305) binding
- V355 (= V346) binding
- D394 (≠ S380) binding
- M395 (≠ E381) mutation to R: Increased rate of aspartate transport; when associated with S-276.
- R397 (= R383) mutation to A: Strongly decreased affinity for aspartate.
- N401 (= N387) binding
- D405 (≠ N391) mutation to N: Strongly decreased affinity for aspartate.
6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
31% identity, 91% coverage: 12:405/433 of query aligns to 21:419/419 of 6x15A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: F46 (≠ L36), F46 (≠ L36), P75 (≠ D65), L91 (≠ V82), F95 (= F86), L130 (vs. gap), I133 (≠ F131), I159 (≠ L158), Y167 (≠ H166), K196 (≠ R187), G200 (≠ M191), I207 (≠ L198), F210 (≠ M201), L250 (≠ I241), I262 (≠ L253), M269 (≠ L260), T334 (= T325), V335 (≠ L326), G336 (≠ S327), T340 (= T331), L343 (≠ A334), M399 (≠ I385)
- binding aspartic acid: S277 (= S268), S278 (= S269), T314 (= T305), G354 (= G345), A358 (= A349), G359 (= G350), D394 (≠ S380), R397 (= R383), T398 (≠ A384)
- binding sodium ion: Y89 (≠ F80), T92 (≠ V83), S93 (= S84), G306 (= G297), T308 (≠ S299), N310 (= N301), N310 (= N301), M311 (≠ L302), D312 (= D303), S349 (= S340), I350 (≠ K341), T352 (≠ A343), N401 (= N387), V402 (≠ I388), D405 (≠ N391)
Sites not aligning to the query:
6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
31% identity, 90% coverage: 12:402/433 of query aligns to 18:413/413 of 6x14A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G59), V83 (≠ I77), I157 (≠ L159), Y164 (≠ H166), K193 (≠ R187), T305 (≠ S299), I306 (≠ F300), I347 (≠ K341)
- binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: M199 (= M193), S275 (= S269), T311 (= T305), G356 (= G350), L384 (= L373), D391 (≠ S380), R394 (= R383)
Sites not aligning to the query:
6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
30% identity, 93% coverage: 2:402/433 of query aligns to 3:408/408 of 6bauA
- binding cysteine: S270 (= S269), M303 (≠ L302), T306 (= T305), A345 (≠ S344), G346 (= G345), V347 (= V346), G351 (= G350), D386 (≠ S380), C389 (≠ R383), T390 (≠ A384), N393 (= N387)
6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
30% identity, 93% coverage: 2:402/433 of query aligns to 3:408/409 of 6bavA
6bmiA Crystal structure of gltph r397c in complex with l-serine (see paper)
29% identity, 90% coverage: 12:402/433 of query aligns to 13:396/396 of 6bmiA
7awmA Structure of the thermostabilized eaat1 cryst mutant in complex with l-asp, three sodium ions and the allosteric inhibitor ucph101 (see paper)
28% identity, 87% coverage: 37:411/433 of query aligns to 57:412/412 of 7awmA
- binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: S88 (≠ V69), G89 (= G70), G92 (= G73), A95 (= A76), V96 (≠ I77), Y99 (≠ F80), M163 (≠ L158), F167 (= F162), F293 (≠ L292), V297 (≠ S296)
- binding aspartic acid: S268 (= S268), S269 (= S269), T306 (= T305), G346 (= G345), I347 (≠ V346), A350 (= A349), G351 (= G350), D380 (≠ S380), R383 (= R383), T384 (≠ A384)
P31596 Excitatory amino acid transporter 2; GLT-1; Sodium-dependent glutamate/aspartate transporter 2; GLUT-R; Solute carrier family 1 member 2 from Rattus norvegicus (Rat) (see paper)
28% identity, 93% coverage: 10:411/433 of query aligns to 52:506/573 of P31596
- K298 (vs. gap) mutation K->H,R: Normal transporter activity.; mutation K->N,T: Reduced transporter activity.
- H326 (≠ M223) mutation H->N,T,K,R: No transporter activity.
7xr6A Structure of human excitatory amino acid transporter 2 (eaat2) in complex with way-213613 (see paper)
30% identity, 93% coverage: 10:411/433 of query aligns to 17:424/424 of 7xr6A
- binding (2S)-2-azanyl-4-[[4-[2-bromanyl-4,5-bis(fluoranyl)phenoxy]phenyl]amino]-4-oxidanylidene-butanoic acid: S280 (= S268), S281 (= S269), T318 (= T305), G363 (= G350), M367 (≠ L354), V385 (≠ L373), D388 (= D376), R395 (= R383), T396 (≠ A384)
- binding dodecyl beta-D-glucopyranoside: V19 (= V12), I20 (≠ L13), W389 (≠ R377)
- binding cholesterol hemisuccinate: R80 (= R71), R84 (≠ K75), I95 (≠ F86), I252 (= I231)
Sites not aligning to the query:
P43006 Excitatory amino acid transporter 2; GLT-1; Sodium-dependent glutamate/aspartate transporter 2; Solute carrier family 1 member 2 from Mus musculus (Mouse) (see paper)
27% identity, 93% coverage: 10:411/433 of query aligns to 52:506/572 of P43006
Sites not aligning to the query:
- 38 modified: S-palmitoyl cysteine; C→S: Severely impairs glutamate uptake activity.
7xr4A Structure of human excitatory amino acid transporter 2 (eaat2) in complex with glutamate (see paper)
29% identity, 93% coverage: 10:411/433 of query aligns to 16:425/425 of 7xr4A
5mjuA Structure of the thermostabilized eaat1 cryst mutant in complex with the competititve inhibitor tfb-tboa and the allosteric inhibitor ucph101 (see paper)
28% identity, 86% coverage: 37:409/433 of query aligns to 49:396/397 of 5mjuA
- binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: L72 (≠ I60), S80 (≠ V69), G81 (= G70), G84 (= G73), Y91 (≠ F80), M156 (≠ L158), F160 (= F162), F286 (≠ L292), V290 (≠ S296)
- binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I64 (= I52), I148 (≠ L150), S262 (= S269), S263 (= S271), A292 (≠ Y298), T293 (≠ S299), M296 (≠ L302), T299 (= T305), G329 (= G342), A336 (= A349), G337 (= G350), D366 (≠ S380), R369 (= R383), N373 (= N387)
7vr7A Inward-facing structure of human eaat2 in the way213613-bound state (see paper)
29% identity, 91% coverage: 10:404/433 of query aligns to 9:401/402 of 7vr7A
- binding (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en: S57 (≠ T55), L58 (≠ V56), L65 (≠ V63), V339 (≠ K341), G340 (= G342), S343 (≠ G345), I344 (≠ V346)
- binding cholesterol: W188 (≠ R194), I227 (≠ K221), F250 (= F250), W257 (≠ L260), M379 (≠ A382), S382 (≠ I385)
- binding (2S)-2-azanyl-4-[[4-[2-bromanyl-4,5-bis(fluoranyl)phenoxy]phenyl]amino]-4-oxidanylidene-butanoic acid: S266 (= S269), M300 (≠ L302), T303 (= T305), Y306 (= Y308), G348 (= G350), L349 (≠ F351), M352 (≠ L354), I366 (≠ L369), L369 (≠ I372), V370 (≠ L373), D373 (= D376), D377 (≠ S380), R380 (= R383), T381 (≠ A384), N384 (= N387)
Sites not aligning to the query:
Q10901 Excitatory amino acid transporter; Sodium-dependent glutamate/ aspartate transporter from Caenorhabditis elegans (see paper)
25% identity, 98% coverage: 1:425/433 of query aligns to 12:483/503 of Q10901
- N177 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- N187 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine
Query Sequence
>N515DRAFT_0013 FitnessBrowser__Dyella79:N515DRAFT_0013
MPAFFRHFYFWVLAAIVAGGLIGHYAPETGVALKPLGDGFIALVKMLIGPIIFLTVVLGI
AGVSDVKKVGRVGAKAILYFEVVSSFALVIGLVVVNTLKPGAGFNATPASLDATAVAKYA
NAAHEQGTVPFLLHLIPKTFSDAFSGDGDLLQVLLLALLFGFAMIHLGERARPVMTFLEA
LSKVFFRIMGMIMRLAPLGAMGAMAFTIGKYGVHSLGPLLKLMGSFYLACILFVVVVLGA
IARATGFSIFKFLRYIREELLLVLGTSSSESALVPLMQKLERLGCSKSVVGLVVPSGYSF
NLDGTNIYLTMAAIFVAQALGVELTLSQELTLLAVAMLTSKGASGVTGAGFITLAATLAV
VPSVPVAGLSLILGIDRFMSEARAITNIIGNGVATVVVSHWEKELDHASLQAALDGRSPS
PAADEAPLLSDAG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory