Comparing N515DRAFT_0030 N515DRAFT_0030 2-methylaconitate cis-trans isomerase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 7 hits to proteins with known functional sites (download)
Q8EJW4 2-methyl-aconitate isomerase; Cis-trans isomerase; EC 5.3.3.- from Shewanella oneidensis (strain MR-1) (see paper)
80% identity, 99% coverage: 1:397/399 of query aligns to 1:395/397 of Q8EJW4
5k87A Crystal structure of malonate bound to methylaconitate isomerase prpf from shewanella oneidensis (see paper)
80% identity, 97% coverage: 10:397/399 of query aligns to 4:387/389 of 5k87A
2pvzA Crystal structure of methylaconitate isomerase prpf from shewanella oneidensis (see paper)
80% identity, 97% coverage: 10:397/399 of query aligns to 4:385/387 of 2pvzA
2pw0A Crystal structure of trans-aconitate bound to methylaconitate isomerase prpf from shewanella oneidensis (see paper)
80% identity, 97% coverage: 10:397/399 of query aligns to 3:383/385 of 2pw0A
2pw0B Crystal structure of trans-aconitate bound to methylaconitate isomerase prpf from shewanella oneidensis (see paper)
71% identity, 97% coverage: 10:397/399 of query aligns to 4:339/341 of 2pw0B
6p3hC Crystal structure of ligu(k66m) bound to substrate (see paper)
38% identity, 96% coverage: 15:396/399 of query aligns to 6:351/352 of 6p3hC
6p3jB Crystal structure of ligu (see paper)
38% identity, 96% coverage: 15:396/399 of query aligns to 11:348/352 of 6p3jB
Sites not aligning to the query:
>N515DRAFT_0030 N515DRAFT_0030 2-methylaconitate cis-trans isomerase
MTNAIKAHAPQLRIPATYLRGGTSKGVFFRLQDLPAAAQVPGAARDALLQRVIGSPDPYG
KQIDGMGGATSSTSKTVIVSRSSRADHDVDYLFGQVAIDKAFVDWSGNCGNLSAAVGPFA
IANGLVDPARVPRDGIAIVRIWQANIGKTIIARVPITDGAVQETGDFELDGVTFPAAEVQ
LEFVDPAAEEDGAGGAMFPTGHVVDDLEVPGVGTLKATMINAGIPTIFVEAAAIGYTGTE
LQDAINSDAKALAMFETIRAHGALRMGLIATLEEIATRQHTPKVAFVAGPADYLASSGKR
VAAGDVDLLVRAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVNVAAGGGERHAVRFGHPSG
TLRVGAEAAQVDGAWTVTKAVMSRSARVLMEGWVRVPAG
Or try a new SitesBLAST search
SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory