SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing N515DRAFT_0084 FitnessBrowser__Dyella79:N515DRAFT_0084 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 16 hits to proteins with known functional sites (download)

4dfdA Crystal structure of had family enzyme bt-2542 (target efi-501088) from bacteroides thetaiotaomicron, magnesium complex
24% identity, 91% coverage: 2:188/206 of query aligns to 5:197/205 of 4dfdA

query
sites
4dfdA

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binding magnesium ion: D11 (= D8), G13 (= G10), D175 (= D166)

Q9HZ62 N-acetylmuramic acid 6-phosphate phosphatase; MurNAc 6-phosphate phosphatase; MurNAc-6P phosphatase; EC 3.1.3.105 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
24% identity, 87% coverage: 2:181/206 of query aligns to 6:186/226 of Q9HZ62

query
sites
Q9HZ62

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D12 (= D8) mutation to A: Loss of activity.

Q51645 (S)-2-haloacid dehalogenase 4A; 2-haloalkanoic acid dehalogenase IVA; Halocarboxylic acid halidohydrolase IVA; L-2-haloacid dehalogenase IVA; EC 3.8.1.2 from Burkholderia cepacia (Pseudomonas cepacia) (see 2 papers)
27% identity, 50% coverage: 79:180/206 of query aligns to 92:191/231 of Q51645

query
sites
Q51645

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N

SN 119:120 (≠ TN 108:109) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
11 active site, Nucleophile
12:13 binding
42 binding

2no5B Crystal structure analysis of a dehalogenase with intermediate complex (see paper)
27% identity, 50% coverage: 79:181/206 of query aligns to 92:192/226 of 2no5B

query
sites
2no5B

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active site: S119 (≠ T108), N120 (= N109), K152 (= K141), S176 (≠ D165), N178 (≠ A167), W180 (≠ R169), D181 (≠ N170)
binding (2s)-2-chloropropanoic acid: N178 (≠ A167), A179 (≠ R168), W180 (≠ R169)

Sites not aligning to the query:

active site: 11, 15, 42
binding (2s)-2-chloropropanoic acid: 20, 35, 38, 39

2b0cA The crystal structure of the putative phosphatase from escherichia coli
21% identity, 93% coverage: 6:196/206 of query aligns to 6:198/199 of 2b0cA

query
sites
2b0cA

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binding 1-O-phosphono-alpha-D-glucopyranose: D8 (= D8), G10 (= G10), S109 (≠ T108), N110 (= N109), K143 (= K141), D168 (= D166)

P0A8Y3 Alpha-D-glucose 1-phosphate phosphatase YihX; Alpha-D-glucose-1-P phosphatase; Alpha-D-glucose-1-phosphatase; Haloacid dehalogenase-like phosphatase 4; HAD4; EC 3.1.3.10 from Escherichia coli (strain K12)
21% identity, 93% coverage: 6:196/206 of query aligns to 4:196/199 of P0A8Y3

query
sites
P0A8Y3

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K

K141 (= K141) binding
D166 (= D166) binding

4ygrA Crystal structure of had phosphatase from thermococcus onnurineus (see paper)
32% identity, 43% coverage: 97:185/206 of query aligns to 98:186/215 of 4ygrA

query
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4ygrA

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active site: T109 (= T108), S110 (= S111), G111 (≠ N112), K141 (= K141), G165 (≠ D165), D166 (= D166), L168 (vs. gap), Q170 (≠ R169), D171 (≠ N170)
binding magnesium ion: D166 (= D166)

Sites not aligning to the query:

active site: 7, 9, 15, 38
binding magnesium ion: 7, 9
binding 2-[n-cyclohexylamino]ethane sulfonic acid: 9, 50, 53, 58, 58, 61

4knvA The crystal structure of apo human hdhd4 from se-mad (see paper)
25% identity, 98% coverage: 5:205/206 of query aligns to 4:215/241 of 4knvA

query
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4knvA

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C

Q

I

D

T

A

D

G

L

R

R

P

T

-

S

-

H

W

W

S

E

E

E

A

A

I

I

A

Q

R

E

L

T

S

K

A

G

E

G

H

-

P

-

A

-

H

-

A

-

P

-

L

-

I

-

A

A

F

N

R

R

E

K

R

L

W

A

E

E

-

C

-

Y

-

F

-

L

-

W

-

K

-

S

-

T

-

R

M

L

L

Q

A

H

G

M

P

T

I

L

A

A

G

E

S

D

V

V

E

K

-

A

L

M

L

L

A

T

E

E

L

L

K

R

A

-

R

K

G

E

V

V

R

R

L

L

Y

L

A

L

L

L

T
|
T

N
|
N

W

G

S

D

N

R

E

Q

T

T

F

Q

P

R

R

E

A

K

L

I

E

E

L

A

Y

C

D

A

F

C

L

Q

H

S

W

Y

F

F

E

D

G

A

I

V

V

V

S

G

G

G

E

E

E

Q

R

R

L

E

I

E

K
|
K

P

P

D

A

P

P

R

S

I

I

Y

F

Q

Y

C

Y

L

C

F

C

E

N

R

L

Y

L

G

G

V

V

E

Q

P

P

S

G

R

D

A

C

L

V

Y

M

I

V

D

G

D
|
D

A

T

-

L

R

E

R

T

N

D

V

I

D

Q

A

G

A

L

A

N

L

A

G

G

M

L

Q

K

A

A

W

-

F

-

Q

-

G

-

A

-

E

-

G

-

L

-

R

T

T

V

W

W

L

I

A

N

S

K

H

N

G

G

L

I

V

V

D

P

D

L

K

K

A

S

T

S

binding magnesium ion: D7 (= D8), D9 (≠ G10), D184 (= D166)
binding phosphate ion: D7 (= D8), L8 (= L9), D9 (≠ G10), T126 (= T108), N127 (= N109), K159 (= K141)

2fdrA Crystal structure of conserved haloacid dehalogenase-like protein of unknown function atu0790 from agrobacterium tumefaciens str. C58
25% identity, 90% coverage: 3:188/206 of query aligns to 3:188/222 of 2fdrA

query
sites
2fdrA

D

D

T

L

V

I

F

F

D
|
D

L

C

G
x
D

G

G

V

V

L

L

I

V

D

D

W

S

N

E

P

I

R

I

Y

-

L

-

Y

-

R

-

Q

-

L

-

F

-

D

-

E

A

A

A

A

Q

M

V

E

E

H

S

F

R

L

L

T

L

K

T

I

E

C

A

T

G

G

Y

P

P

W

I

N

S

E

V

Q

E

D

M

-

G

-

E

-

R

-

F

A

A

G

G

R

M

P

T

W

W

S

K

E

N

A

I

I

L

A

-

R

-

L

L

S

Q

A

V

E

E

H

S

P

E

A

A

H

S

A

I

P

P

L

L

I

S

A

A

A

S

F

L

R

L

E

D

R

K

W

S

E

E

E

K

M

L

L

L

A

D

G

M

P

R

I

L

A

E

G

R

S

D

V

V

E

K

L

I

A

D

E

G

L

V

K

K

A

F

R

A

G

L

V

S

R

R

L

L

Y

T

A

T

-

P

-

R

-

C

-

I

L

C

T

S

N

N

W

S

S

S

N

S

E

H

T

R

F

L

P

D

R

M

A

M

L

L

E

T

L

K

Y

V

D

G

F

L

L

K

H

P

W

Y

F

F

E

A

G

P

I

H

V

I

V

Y

S

S

-

A

-

K

-

D

-

L

G

G

E

A

E

D

R

R

L

-

I

V

K

K

P

P

D

K

P

P

R

D

I

I

Y

F

Q

L

C

H

L

G

F

A

E

A

R

Q

Y

F

G

G

V

V

E

S

P

P

S

D

R

R

A

V

L

V

Y

V

I

V

D

E

D
|
D

A

S

R

V

R

H

N

G

V

I

D

H

A

G

A

A

A

R

A

A

L

A

G

G

M

M

Q

R

A

V

W

I

W

G

F

F

Q

T

G

G

A

A

E

S

binding magnesium ion: D8 (= D8), D10 (≠ G10), D166 (= D166)

3r09A Crystal structure of probable had family hydrolase from pseudomonas fluorescens pf-5 with bound mg
35% identity, 46% coverage: 86:180/206 of query aligns to 70:165/197 of 3r09A

query
sites
3r09A

P

P

I

A

A

P

G

G

S

A

V

V

E

E

L

L

L

V

A

R

E

E

L

L

K

A

A

G

R

R

G

G

V

Y

R

R

L

L

Y

G

A

I

L

L

T

T

N

R

W

N

S

A

N

R

E

E

T

L

F

A

P

H

R

V

A

T

L

L

E

E

L

A

Y

I

D

G

F

L

L

A

H

D

W

C

F

F

-

A

E

E

G

A

I

D

V

V

V

L

S

G

G

R

E

D

E

E

R

A

L

P

I

P

K

K

P

P

D

H

P

P

R

G

I

G

Y

L

Q

L

C

K

L

L

F

A

E

E

R

A

Y

W

G

D

V

V

E

S

P

P

S

S

R

R

A

M

L

V

Y

M

I

V

D

G

D
|
D

A

Y

R

R

R

F

N

D

V

L

D

D

A

C

A

G

A

R

A

A

L

A

G

G

M

T

Q

R

binding magnesium ion: D151 (= D166)

Sites not aligning to the query:

binding magnesium ion: 11, 13

5mwaA Human seh phosphatase in complex with 3-4-3,4-dichlorophenyl-5-phenyl- 1,3-oxazol-2-yl-benzoic-acid (see paper)
27% identity, 45% coverage: 97:188/206 of query aligns to 106:193/209 of 5mwaA

query
sites
5mwaA

L

L

K

R

A

K

R

K

G

G

V

F

R

T

L

T

Y

A

A

I

L

L

T

T

N
|
N

-

T

W
|
W

S

L

N

D

E

D

T

R

F

L

P

M

R

C

A

E

L

L

E

K

L

M

Y

H

D

-

F

-

L

-

H

-

W

-

F

F

E

D

G

F

I

L

V

I

V

E

S

S

G

C

E

Q

E

V

R

G

L

M

I

V

K

K

P

P

D

E

P

P

R

Q

I

I

Y

Y

Q

K

C

F

L

L

F

L

E

D

R

T

Y

L

G

K

V

A

E

S

P

P

S

S

R

E

A

V

L

V

Y

F

I

L

D

D

D
|
D

A

I

R

G

R

A

N

N

V

L

D

K

A

P

A

A

A

R

A

D

L

L

G

G

M

M

Q

V

A

T

W

I

W

L

F

V

Q

Q

G

D

A

T

E

D

Sites not aligning to the query:

binding 3-[4-(3,4-dichlorophenyl)-5-phenyl-1,3-oxazol-2-yl]benzoic acid: 16, 22, 24, 46, 58, 59, 68, 86, 89, 90, 93
binding magnesium ion: 14, 16

8wbtA Crystal structure of cis-epoxysuccinate hydrolases klcesh[l] mutant d48n complexed with l-ta (see paper)
26% identity, 67% coverage: 43:180/206 of query aligns to 62:195/237 of 8wbtA

query
sites
8wbtA

P

P

W

W

N

T

E

T

Q

T

Q

D

D

-

A

-

G

-

R

R

P

I

W

Y

S

R

E

E

A

A

I

L

A

D

R

G

L

I

S

L

A

A

E

N

H

H

P

P

A

W

H

G

A

A

P

S

L

L

I

N

A

S

A

A

F

D

R

R

E

D

R

E

-

L

-

N

-

S

-

L

W

W

E

S

E

K

M

L

L

I

A

-

G

-

P

P

I

W

A

D

G

D

S

T

V

A

E

P

L

G

L

L

A

A

E

R

L

L

K

R

A

S

R

K

G

-

V

-

R

-

L

-

Y

Y

A

I

L

T

T

S

N

T

W

L

S
|
S

N
|
N

E

G

T

S

F

M

P

A

R

S

A

V

L

L

E

R

L

I

Y

S

D

K

F

L

L

G

H

A

W

L

-

P

F

F

E

D

G

A

I

I

V

L

V

T

S

A

G

E

E

L

E

V

R

R

L

S

I

S

K
|
K

P

P

D

D

P

P

R

K

I

V

Y

Y

Q

Q

C

L

L

A

F

L

E

D

R

S

Y

V

G

G

V

I

E

E

P

A

S

H

R

Q

A

A

L

M

Y

M

I

V

D

A

D

C

A

H

R

K

R
x
Y

N

D

V

L

D

Q

A

A

A

K

A

R

L

L

G

G

M

F

Q

K

binding l(+)-tartaric acid: S125 (= S111), N126 (= N112), K156 (= K141), Y184 (≠ R169)

Sites not aligning to the query:

binding l(+)-tartaric acid: 9, 10, 11

4uauA Crystal structure of cbby (mutant d10n) from rhodobacter sphaeroides in complex with xylulose-(1,5)bisphosphate, crystal form ii (see paper)
26% identity, 90% coverage: 1:186/206 of query aligns to 1:196/226 of 4uauA

query
sites
4uauA

M

M

I

I

D

E

T

A

V

I

V

L

F

F

D
|
D

L
x
V

G
x
N

G

G

V

T

L

L

I

A

D

E

W
x
T

N
x
E

P

E

R

-

Y

-

L

L

Y
x
H

R

R

Q

R

L

A

F

F

D

N

D

-

E

-

A

-

M

-

E

E

H

T

F

F

L

A

T

A

K

L

I

G

C

V

T

D

G

W

P

F

W

W

N

D

E

R

Q

E

-

Y

-

R

-

E

-

L

-
x
T

-

T

D
x
T

A
x
G

G
|
G

R

K

P

E

W
x
R

S

I

E

A

A

R

I

F

A

L

R

R

L

H

S

Q

A

K

E

G

H

D

P

P

A

A

H

P

A

L

P

P

L

I

I

A

A

D

A

I

F
x
H

R

R

E

A

R
x
K

W

T

E

E

E

R

M

F

L

V

A

A

-

L

-

M

-

A

-

E

G

G

P

E

I

I

A

A

-

L

-

R

-

P

G

G

S

I

V

A

E

D

L

L

L

I

A

A

E

E

L

A

K

K

A

R

R

A

G

G

V

I

R

R

L

L

Y

A

A

V

L

A

T
|
T

N
x
T

W
x
T

S
|
S

-

L

-

P

-

N

N

V

E

E

T

A

F

L

P

C

R

R

A

A

L

C

E

F

L

G

Y

H

D

P

F

A

L

R

H

E

W

I

F

F

E

D

G

V

I

I

V

A

S

G

G

D

E

M

E

V

R

A

L

E

I

K

K
|
K

P

P

D

S

P

P

R

D

I

I

Y

Y

Q

R

C

L

L

A

F

L

E

R

R

E

Y

L

G

D

V

V

E

P

P

P

S

E

R

R

A

A

L

V

Y

A

I

L

D
x
E

D
|
D

A

S

R

L

R

N

N

G

V

L

D

R

A

A

A

K

A

G

L

A

G

G

M

L

Q

R

A

C

W

I

W

V

F

S

Q

P

G

G

active site: D8 (= D8), V9 (≠ L9), N10 (≠ G10), T16 (≠ W16), T47 (vs. gap), T115 (= T108), T116 (≠ N109), K151 (= K141), E175 (≠ D165), D176 (= D166)
binding magnesium ion: D8 (= D8), N10 (≠ G10), D176 (= D166)
binding xylulose-1,5-bisphosphate: D8 (= D8), V9 (≠ L9), N10 (≠ G10), E17 (≠ N17), H20 (≠ Y22), T49 (≠ D49), G50 (≠ A50), G51 (= G51), R54 (≠ W54), H75 (≠ F75), K78 (≠ R78), T115 (= T108), T116 (≠ N109), T117 (≠ W110), S118 (= S111)

P95649 Protein CbbY; RuCbby; EC 3.1.3.- from Cereibacter sphaeroides (Rhodobacter sphaeroides) (see paper)
26% identity, 90% coverage: 1:186/206 of query aligns to 1:196/230 of P95649

query
sites
P95649

M

M

I

I

D

E

T

A

V

I

V

L

F

F

D

D

L

V

G
x
D

G

G

V

T

L

L

I

A

D

E

W

T

N
x
E

P

-

R

-

Y

E

L

L

Y
x
H

R

R

Q

R

L

A

F

F

D

N

D

-

E

-

A

-

M

-

E

E

H

T

F

F

L

A

T

A

K

L

I

G

C

V

T

D

G

W

P

F

W

W

N

D

E

R

Q

E

-
x
Y

-

R

-

E

-

L

-

T

D

T

A

G

G

G

R

K

P

E

W
x
R

S

I

E

A

A

R

I

F

A

L

R

R

L

H

S

Q

A

K

E

G

H

D

P

P

A

A

H

P

A

L

P

P

L

I

I

A

A

D

A

I

F

H

R

R

E

A

R
x
K

W

T

E

E

E

R

M

F

L

V

A

A

-

L

-

M

-

A

-

E

G

G

P

E

I

I

A

A

-

L

-

R

-

P

G

G

S

I

V

A

E

D

L

L

L

I

A

A

E

E

L

A

K

K

A

R

R

A

G

G

V

I

R

R

L

L

Y

A

A

V

L

A

T

T

N

T

W

T

S

S

-

L

-

P

-

N

N

V

E

E

T

A

F

L

P

C

R

R

A

A

L

C

E

F

L

G

Y

H

D

P

F

A

L

R

H

E

W

I

F

F

E

D

G

V

I

I

V

A

S

G

G

D

E

M

E

V

R

A

L

E

I

K

K

K

P

P

D

S

P

P

R

D

I

I

Y

Y

Q

R

C

L

L

A

F

L

E

R

R

E

Y

L

G

D

V

V

E

P

P

P

S

E

R

R

A

A

L

V

Y

A

I

L

D

E

D

D

A

S

R

L

R

N

N

G

V

L

D

R

A

A

A

K

A

G

L

A

G

G

M

L

Q

R

A

C

W

I

W

V

F

S

Q

P

G

G

D10 (≠ G10) mutation to N: Loss of catalytic activity.
E17 (≠ N17) mutation to A: 40% to 80% decreased catalytic activity with xylulose-1,5-bisphosphate, but no effect on activity with ribulose-1,5-bisphosphate.
H20 (≠ Y22) mutation to A: 40% to 80% decreased catalytic activity with xylulose-1,5-bisphosphate, but no effect on activity with ribulose-1,5-bisphosphate.
Y42 (vs. gap) mutation to A: 40% to 80% decreased catalytic activity with xylulose-1,5-bisphosphate, but no effect on activity with ribulose-1,5-bisphosphate.
R54 (≠ W54) mutation to A: 97% decreased catalytic activity with xylulose-1,5-bisphosphate, but no effect on activity with ribulose-1,5-bisphosphate.
K78 (≠ R78) mutation to A: 40% to 80% decreased catalytic activity with xylulose-1,5-bisphosphate, but no effect on activity with ribulose-1,5-bisphosphate.

4uasA Crystal structure of cbby from rhodobacter sphaeroides in complex with phosphate (see paper)
26% identity, 90% coverage: 1:186/206 of query aligns to 1:196/225 of 4uasA

query
sites
4uasA

M

M

I

I

D

E

T

A

V

I

V

L

F

F

D
|
D

L
x
V

G
x
D

G

G

V

T

L

L

I

A

D

E

W
x
T

N

E

P

-

R

-

Y

E

L

L

Y

H

R

R

Q

R

L

A

F

F

D

N

D

-

E

-

A

-

M

-

E

E

H

T

F

F

L

A

T

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L

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G

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D

G

W

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F

W

W

N

D

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Y

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x
T

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T

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T

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G

G

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A

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P

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A

D

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H

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A

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G

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active site: D8 (= D8), V9 (≠ L9), D10 (≠ G10), T16 (≠ W16), T47 (vs. gap), T115 (= T108), T116 (≠ N109), K151 (= K141), E175 (≠ D165), D176 (= D166)
binding magnesium ion: D8 (= D8), D10 (≠ G10), D176 (= D166)
binding phosphate ion: D8 (= D8), V9 (≠ L9), D10 (≠ G10), T115 (= T108), T116 (≠ N109), K151 (= K141)

P80299 Bifunctional epoxide hydrolase 2; EC 3.3.2.10; EC 3.1.3.76 from Rattus norvegicus (Rat) (see paper)
27% identity, 41% coverage: 95:179/206 of query aligns to 110:198/554 of P80299

query
sites
P80299

A

A

E

A

L

L

K

K

A

K

R

K

G

G

V

F

R

T

L

T

Y

C

A

I

L

V

T

T

N

N

-

N

W

W

S

L

N

D

E

D

T

S

F

D

P

K

R

R

-

D

-

I

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A

A

L

Q

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L

M

Y

C

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H

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D

K

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A

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G

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M

Sites not aligning to the query:

333 active site, Nucleophile; D→G: Loss of epoxide hydrolase activity.
334 W→F: Slight reduction of epoxide hydrolase activity.
433 mutation E->Q,K: Slight loss of epoxide hydrolase activity.
434 D→Y: Slight loss of epoxide hydrolase activity.
493 mutation E->Q,K: No effect.
495 D→H: Loss of epoxide hydrolase activity.
517 H→Y: Reduces epoxide hydrolase activity by 50%. Loss of activity; when associated with Y-521.
521 C→Y: Loss of epoxide hydrolase activity; when associated with Y-517.
523 active site, Proton acceptor; mutation H->D,N,Y: Loss of epoxide hydrolase activity.
526 Q→H: Reduces epoxide hydrolase activity by 80%; when associated with T-542.
540 mutation W->L,S: Reduces epoxide hydrolase activity by 95%.
542 K→T: Reduces epoxide hydrolase activity by 80%; when associated with H-526.

Query Sequence

>N515DRAFT_0084 FitnessBrowser__Dyella79:N515DRAFT_0084
MIDTVVFDLGGVLIDWNPRYLYRQLFDDEAAMEHFLTKICTGPWNEQQDAGRPWSEAIAR
LSAEHPAHAPLIAAFRERWEEMLAGPIAGSVELLAELKARGVRLYALTNWSNETFPRALE
LYDFLHWFEGIVVSGEERLIKPDPRIYQCLFERYGVEPSRALYIDDARRNVDAAAALGMQ
AWWFQGAEGLRTWLASHGLVDDKATA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory