SitesBLAST
Comparing N515DRAFT_0382 N515DRAFT_0382 MFS transporter, sugar porter (SP) family to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P0AGF4 D-xylose-proton symporter; D-xylose transporter from Escherichia coli (strain K12) (see paper)
37% identity, 97% coverage: 14:470/472 of query aligns to 7:474/491 of P0AGF4
- F24 (= F31) mutation to A: Decreases xylose transport.
- G83 (= G82) mutation to A: Abolishes xylose transport.
- R133 (= R114) mutation R->C,H,L: Abolishes xylose transport.
- E153 (= E134) mutation to A: Abolishes xylose transport.
- R160 (= R141) mutation to A: Abolishes xylose transport.
- Q168 (= Q149) binding ; mutation to A: Abolishes xylose transport.
- Q288 (= Q280) mutation to A: Abolishes xylose transport.
- QQ 288:289 (= QQ 280:281) binding
- Q289 (= Q281) mutation to A: Strongly decreases xylose transport.
- N294 (= N286) binding ; mutation to A: Abolishes xylose transport.
- Y298 (= Y290) mutation to A: Abolishes xylose transport.
- N325 (= N317) mutation to A: No effect on xylose transport.
- G340 (= G332) mutation to A: Abolishes xylose transport.
- R341 (= R333) mutation R->A,W: Abolishes xylose transport.
- W392 (= W395) binding ; mutation to A: Abolishes xylose transport.
- E397 (= E400) mutation to A: Abolishes xylose transport.
- R404 (= R407) mutation to A: Strongly decreases xylose transport.
- Q415 (= Q418) binding
- W416 (= W419) mutation to A: Strongly decreases xylose transport.
4gc0A The structure of the mfs (major facilitator superfamily) proton:xylose symporter xyle bound to 6-bromo-6-deoxy-d-glucose (see paper)
37% identity, 97% coverage: 14:470/472 of query aligns to 3:470/475 of 4gc0A
4gbzA The structure of the mfs (major facilitator superfamily) proton:xylose symporter xyle bound to d-glucose (see paper)
37% identity, 97% coverage: 14:470/472 of query aligns to 3:470/475 of 4gbzA
- binding beta-D-glucopyranose: F20 (= F31), Q164 (= Q149), Q171 (≠ I156), Q284 (= Q280), Q285 (= Q281), N290 (= N286), L293 (≠ F289), F379 (= F386), G384 (= G391), W388 (= W395), Q411 (= Q418)
4gbyA The structure of the mfs (major facilitator superfamily) proton:xylose symporter xyle bound to d-xylose (see paper)
37% identity, 97% coverage: 14:470/472 of query aligns to 3:470/475 of 4gbyA
A0A0H2VG78 Glucose transporter GlcP; Glucose/H(+) symporter from Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) (see paper)
34% identity, 93% coverage: 34:470/472 of query aligns to 22:433/446 of A0A0H2VG78
- D22 (= D34) mutation to N: Affects symport activity. May function as an uniporter.
- R102 (= R114) mutation to A: Loss of transport activity.
- I105 (≠ G117) mutation to S: Affects symport activity. May function as an uniporter.
- E122 (= E134) mutation to A: Loss of transport activity.
- Q137 (= Q149) mutation to A: Loss of transport activity.
- Q250 (= Q280) mutation to A: Loss of transport activity.
- Q251 (= Q281) mutation to A: Loss of transport activity.
- N256 (= N286) mutation to A: Loss of transport activity.
- W357 (= W395) mutation to A: Loss of transport activity.
Q8VZR6 Inositol transporter 1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
32% identity, 96% coverage: 20:472/472 of query aligns to 34:477/509 of Q8VZR6
Sites not aligning to the query:
- 479:509 mutation Missing: Leads to endoplasmic reticulum relocalization.
- 481:482 ER→AA: No effect on targeting.
- 500:509 mutation Missing: Leads to endoplasmic reticulum relocalization.
- 502:504 mutation LLE->AAA,SSS: Leads to plasma membrane relocalization.
P38695 Probable glucose transporter HXT5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
29% identity, 98% coverage: 10:472/472 of query aligns to 76:547/592 of P38695
Sites not aligning to the query:
- 61 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
P17809 Solute carrier family 2, facilitated glucose transporter member 1; Glucose transporter type 1, erythrocyte/brain; GLUT-1; GT1 from Mus musculus (Mouse) (see 3 papers)
29% identity, 97% coverage: 12:470/472 of query aligns to 7:463/492 of P17809
- N45 (≠ G50) modified: carbohydrate, N-linked (GlcNAc...) asparagine
Sites not aligning to the query:
- 485 P→L: Lethality immediately after birth in knockin mice; caused by creation of a dileucine internalization motif that promotes mislocalization of the protein.
P11169 Solute carrier family 2, facilitated glucose transporter member 3; Glucose transporter type 3, brain; GLUT-3 from Homo sapiens (Human) (see paper)
27% identity, 98% coverage: 9:472/472 of query aligns to 2:463/496 of P11169
- Q159 (= Q149) binding
- QLS 277:279 (≠ SVF 277:279) Important for selectivity against fructose; mutation to HVA: Confers moderate fructose transport activity.
- QQ 280:281 (= QQ 280:281) binding
- N286 (= N286) binding
- N315 (= N317) binding
- E378 (≠ G387) binding
- W386 (= W395) binding
P39003 High-affinity hexose transporter HXT6 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
30% identity, 99% coverage: 4:471/472 of query aligns to 52:525/570 of P39003