Comparing N515DRAFT_0495 FitnessBrowser__Dyella79:N515DRAFT_0495 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P13009 Methionine synthase; 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS; EC 2.1.1.13 from Escherichia coli (strain K12) (see 5 papers)
64% identity, 99% coverage: 6:895/895 of query aligns to 340:1227/1227 of P13009
Sites not aligning to the query:
Q99707 Methionine synthase; MS; 5-methyltetrahydrofolate--homocysteine methyltransferase; Cobalamin-dependent methionine synthase; Vitamin-B12 dependent methionine synthase; EC 2.1.1.13 from Homo sapiens (Human) (see 6 papers)
54% identity, 100% coverage: 5:895/895 of query aligns to 354:1265/1265 of Q99707
Sites not aligning to the query:
3bulA E. Coli i690c/g743c meth c-terminal fragment (649-1227) (see paper)
60% identity, 65% coverage: 318:895/895 of query aligns to 4:577/577 of 3bulA
3ivaA Structure of the b12-dependent methionine synthase (meth) c-teminal half with adohcy bound (see paper)
60% identity, 64% coverage: 318:893/895 of query aligns to 4:575/576 of 3ivaA
3k13C Structure of the pterin-binding domain metr of 5- methyltetrahydrofolate-homocysteine methyltransferase from bacteroides thetaiotaomicron
67% identity, 32% coverage: 22:305/895 of query aligns to 4:286/287 of 3k13C
4cczA Crystal structure of human 5-methyltetrahydrofolate-homocysteine methyltransferase, the homocysteine and folate binding domains
61% identity, 33% coverage: 5:301/895 of query aligns to 314:610/611 of 4cczA
1mskA Methionine synthase (activation domain) (see paper)
55% identity, 36% coverage: 572:895/895 of query aligns to 4:327/327 of 1mskA
6bdyA Crystal structure of the meth reactivation domain bound to sinefungin (see paper)
55% identity, 36% coverage: 572:893/895 of query aligns to 4:325/326 of 6bdyA
1bmtA How a protein binds b12: a 3.O angstrom x-ray structure of the b12- binding domains of methionine synthase (see paper)
68% identity, 27% coverage: 318:562/895 of query aligns to 4:244/246 of 1bmtA
8g3hA Structure of cobalamin-dependent methionine synthase (meth) in a resting state (see paper)
38% identity, 54% coverage: 25:510/895 of query aligns to 335:806/841 of 8g3hA
Sites not aligning to the query:
8sseA Methionine synthase, c-terminal fragment, cobalamin and reactivation domains from thermus thermophilus hb8
34% identity, 60% coverage: 322:860/895 of query aligns to 1:506/507 of 8sseA
5vooA Methionine synthase folate-binding domain with methyltetrahydrofolate from thermus thermophilus hb8 (see paper)
36% identity, 32% coverage: 21:306/895 of query aligns to 2:281/282 of 5vooA
2i2xB Crystal structure of methanol:cobalamin methyltransferase complex mtabc from methanosarcina barkeri (see paper)
33% identity, 21% coverage: 321:507/895 of query aligns to 45:216/258 of 2i2xB
Sites not aligning to the query:
Q46EH4 Methanol--corrinoid protein; Methanol:corrinoid methyltransferase 1 subunit of 27 kDa; MT1 subunit 27 kDa from Methanosarcina barkeri (strain Fusaro / DSM 804) (see paper)
33% identity, 21% coverage: 321:507/895 of query aligns to 45:216/258 of Q46EH4
Sites not aligning to the query:
7xcnP Crystal structure of the mttb-mttc complex at 2.7 a resolution (see paper)
31% identity, 19% coverage: 336:507/895 of query aligns to 12:188/215 of 7xcnP
Sites not aligning to the query:
3ezxA Structure of methanosarcina barkeri monomethylamine corrinoid protein
31% identity, 20% coverage: 325:506/895 of query aligns to 7:185/212 of 3ezxA
Sites not aligning to the query:
4jgiB 1.5 angstrom crystal structure of a novel cobalamin-binding protein from desulfitobacterium hafniense dcb-2 (see paper)
33% identity, 16% coverage: 364:502/895 of query aligns to 45:174/206 of 4jgiB
Sites not aligning to the query:
4o1eA Structure of a methyltransferase component in complex with mthf involved in o-demethylation (see paper)
25% identity, 27% coverage: 21:264/895 of query aligns to 4:232/271 of 4o1eA
Sites not aligning to the query:
4o1fA Structure of a methyltransferase component in complex with thf involved in o-demethylation (see paper)
25% identity, 27% coverage: 21:264/895 of query aligns to 1:228/267 of 4o1fA
3bofA Cobalamin-dependent methionine synthase (1-566) from thermotoga maritima complexed with zn2+ and homocysteine (see paper)
26% identity, 27% coverage: 22:264/895 of query aligns to 315:538/560 of 3bofA
Sites not aligning to the query:
>N515DRAFT_0495 FitnessBrowser__Dyella79:N515DRAFT_0495
MSTRHTRLSGLEPLVITPDLLFVNVGERTNVTGSAQFRKLIKEDRYEEAVDVARQQVASG
AQIIDVNMDEGLIDSEAAMTRFLNLIAAEPDIARVPVMVDSSKWTVLEAGLRCLQGKGIV
NSISMKEGEELFLEHARKVQQYGAAVVVMAFDEQGQADTCERKVEICSRAYALLTEQLDF
PPEDIVFDPNIFAIATGIEEHNNYAVDFIEATRELKRRFPLSHISGGVSNVSFSFRGNNT
VREAIHSVFLYHAIKAGMDMGIVNAGALMIYDDVPAELRERVEDVVLNRRPDATERLLEI
ADNYKARKGEVVVENLAWREKPVRERLSHALVHGIDAFVDADTEEARQLATRPLDVIEGP
LMDGMNVVGDLFGAGKMFLPQVVKSARVMKKAVAYLLPYIEEEKARTGDVGKNNGTIVMA
TVKGDVHDIGKNIVGVVLRCNNFDVIDLGVMVPAQKILDAAREHNADLIGLSGLITPSLE
EMSHVAREMQRQEFSIPLLIGGATTSRAHTALKIDPHYKAPTVWVKDASRAVGVAQSLVS
KDLVDAFMAKVRADYEEVRERHRNRGPGKSLVPLEKARAQRYTCDWAGYAPPQPRQPGVT
VFDAYDLAELREYIDWTPFFQAWELAGRYPAILKDEIVGTQASELFRDAQAMLDRIVAER
WLTARAVIGFWRAAQVGDDTEVYGEDGRKLAVLRHLRQQADKPADRPDFSLGDFIAPKEA
GKQDWVGAFAVTAGIGIEEHVARFEAAHDDYSSILLKALADRLAEAFAERMHQRVRREFW
GYAPDEALDNEALIDEKYRGIRPAPGYPACPDHTEKSTLFKLLDATANAGIELTEGYAMY
PTAAVSGWYFSHPDSQYFVVGRLTREQVEDYAKRKGWTREEAERWLAPNLDYDPD
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SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory