SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing N515DRAFT_1248 N515DRAFT_1248 ABC-2 type transport system ATP-binding protein to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4yerA Crystal structure of an abc transporter atp-binding protein (tm_1403) from thermotoga maritima msb8 at 2.35 a resolution
39% identity, 82% coverage: 12:268/315 of query aligns to 5:261/285 of 4yerA

query
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4yerA

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binding adenosine-5'-diphosphate: F14 (≠ Y21), F17 (= F25), A19 (= A27), P38 (= P46), N39 (= N47), G40 (= G48), A41 (= A49), G42 (= G50), K43 (= K51), T44 (= T52), T45 (= T53), T135 (= T141), F136 (≠ L142), S137 (= S143)

8ee6A Cryo-em structure of human abca7 in pe/ch nanodiscs (see paper)
35% identity, 99% coverage: 1:311/315 of query aligns to 612:932/1808 of 8ee6A

query
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binding phosphothiophosphoric acid-adenylate ester: F633 (≠ Y21), S636 (≠ G24), A640 (= A27), N660 (= N47), G661 (= G48), A662 (= A49), G663 (= G50), K664 (= K51), T665 (= T52), T666 (= T53)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 607, 608, 1507, 1514, 1535, 1536, 1537, 1538, 1539, 1540, 1579

Q9BZC7 ATP-binding cassette sub-family A member 2; ATP-binding cassette transporter 2; ATP-binding cassette 2; EC 7.6.2.- from Homo sapiens (Human) (see paper)
35% identity, 70% coverage: 1:219/315 of query aligns to 979:1199/2435 of Q9BZC7

query
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Q9BZC7

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Sites not aligning to the query:

271 modified: N5-methylglutamine; Q→R: Abolishes methylation by N6AMT1.

7tbwA The structure of atp-bound abca1
31% identity, 95% coverage: 11:310/315 of query aligns to 753:1046/1928 of 7tbwA

query
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7tbwA

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binding adenosine-5'-triphosphate: Y763 (= Y21), D765 (≠ G23), K768 (≠ Q26), A770 (= A27), H789 (≠ P46), N790 (= N47), G791 (= G48), G793 (= G50), K794 (= K51), T795 (= T52), T796 (= T53), Q835 (= Q92), K880 (= K134), Q887 (≠ T141), S889 (= S143), G891 (= G145), Q913 (≠ E167), H944 (= H199)
binding magnesium ion: Q835 (= Q92), D912 (= D166), Q913 (≠ E167)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 1630, 1633, 1657, 1658, 1660, 1661, 1662, 1663, 1702, 1753, 1755, 1756, 1757, 1779, 1783, 1811
binding cholesterol: 213, 218, 219, 352, 360, 367
binding magnesium ion: 1662, 1702, 1779

8eopA Cryo-em structure of nanodisc reconstituted human abca7 eq mutant in atp bound closed state (see paper)
39% identity, 68% coverage: 7:219/315 of query aligns to 646:859/1687 of 8eopA

query
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8eopA

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L

binding adenosine-5'-triphosphate: F659 (≠ Y21), S662 (≠ G24), A666 (= A27), N686 (= N47), G687 (= G48), G689 (= G50), K690 (= K51), T691 (= T52), T692 (= T53), Q731 (= Q92), H782 (≠ T141), S784 (= S143), M787 (= M146), H839 (= H199)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 1461, 1468, 1488, 1489, 1491, 1492, 1493, 1494, 1533, 1577, 1584, 1586, 1610
binding magnesium ion: 1493, 1533

7roqA Alternative structure of human abca1
30% identity, 98% coverage: 1:310/315 of query aligns to 768:1079/1831 of 7roqA

query
sites
7roqA

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Sites not aligning to the query:

binding cholesterol: 681, 688, 1106, 1426, 1526, 1527, 1528, 1531

O95477 Phospholipid-transporting ATPase ABCA1; ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter 1; ABC-1; ATP-binding cassette 1; Cholesterol efflux regulatory protein; EC 7.6.2.1 from Homo sapiens (Human) (see 35 papers)
33% identity, 74% coverage: 1:233/315 of query aligns to 888:1123/2261 of O95477

query
sites
O95477

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D917 (≠ K29) to Y: in a colorectal cancer sample; somatic mutation
T929 (≠ F41) to I: in TGD; moderately decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; loss protein subcellular localization to the plasma membrane
N935 (= N47) to S: in TGD; moderately decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; dbSNP:rs28937313
K939 (= K51) mutation to M: Inhibits ATPase activity; when associated with M-1952. Decreases translocase activity; when associated with M-1952. Does not affect protein subcellular localization in plasma membrane and endosome; when associated with M-1952.
S1042 (≠ L151) modified: Phosphoserine; by PKA
P1065 (≠ V174) natural variant: P -> S
M1091 (≠ I201) to T: in FHA1; loss of localization to plasma membrane; decreased cholesterol efflux; decreased phospholipid efflux
C1110 (≠ I220) modified: S-palmitoyl cysteine; mutation to S: Decreased palmitoylation; when associated with S-3, S-23 and S-1111.
C1111 (≠ L221) modified: S-palmitoyl cysteine; mutation to S: Decreased palmitoylation; when associated with S-3, S-23 and S-1110.

Sites not aligning to the query:

3 modified: S-palmitoyl cysteine; C→S: Mild decrease of palmitoylation. Loss of localization to plasma membrane. Decreased cholesterol efflux. Decreased phospholipid efflux. Decreased palmitoylation; when associated with S-23, S-1110 and S-1111.
23 modified: S-palmitoyl cysteine; C→S: Mild decrease of palmitoylation. Loss of localization to plasma membrane. Decreased palmitoylation; when associated with S-3, S-1110 and S-1111.
74 I→C: 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with C-371.; I→K: 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with E-371.
75 modified: Disulfide link with 309
85 P → L: in FHA1; Alabama; dbSNP:rs145183203
98 modified: carbohydrate, N-linked (GlcNAc...) asparagine
100 S→C: Highly decreased protein abundance. Highly decreased ATPase activity. Highly decreased phospholipid translocase activity.
210 E → D: in a colorectal cancer sample; somatic mutation
219 R → K: associated with a decreased severity of CAD; dbSNP:rs2230806
230 R → C: in dbSNP:rs9282541
244 modified: carbohydrate, N-linked (GlcNAc...) asparagine
248 P → A: in dbSNP:rs142625938
255 A → T: in TGD; deficient cellular cholesterol efflux; dbSNP:rs758100110
304 V→C: No effect on phospholipid and cholesterol efflux or localization to cell membrane; when associated with C-308.
308 V→C: No effect on phospholipid and cholesterol efflux or localization to cell membrane; when associated with C-304.
309 modified: Disulfide link with 75
364 S → C: in dbSNP:rs775035559
371 I→C: No effect on phospholipid and cholesterol efflux or localization to cell membrane. 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with C-74 or C-375.; I→E: 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-74.
375 L→C: 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with C-371.
399 V → A: in dbSNP:rs9282543
401 K → Q: in dbSNP:rs138487227
496 R → W: associated with increased plasma HDL cholesterol; dbSNP:rs147675550
568 K→A: 60-65% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane.
573 Y→F: No effect on phospholipid and cholesterol efflux and on localization to cell membrane.
581 D→K: 80-85% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-584 and K-585.
583 F→K: 90-95% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with E-590.
584 E→K: 80-85% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-581 and K-585.
585 D→K: 80-85% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-581 and K-584.
590 W → S: in TGD; moderately decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; dbSNP:rs137854496; W→E: 90-95% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-583.
593 F→L: Moderately decreased protein abundance. Highly decreased ATPase activity. Highly decreased phospholipid translocase activity.
638 R → Q: associated with reduced plasma HDL cholesterol; dbSNP:rs374190304
771 V → M: associated with HDL cholesterol; dbSNP:rs2066718
774 T → P: in dbSNP:rs35819696; natural variant: T -> S
776 K → N: may be associated with increased risk of ischemic heart disease; dbSNP:rs138880920
815 E → G: associated with reduced plasma HDL cholesterol; dbSNP:rs145582736
825 V → I: associated with higher plasma cholesterol; dbSNP:rs2066715
883 I → M: associated with higher plasma cholesterol; dbSNP:rs2066714
1172 E → D: associated with premature coronary heart disease; dbSNP:rs33918808
1181 S → F: associated with reduced plasma HDL cholesterol; dbSNP:rs76881554
1216 G → V: in dbSNP:rs562403512
1341 R → T: associated with reduced plasma HDL cholesterol; dbSNP:rs147743782
1376 S → G: in dbSNP:rs145689805
1379 L → F: in TGD; the mutant protein is retained in the endoplasmic reticulum while the wild-type protein is located at the plasma membrane
1407 A → T: in a colorectal cancer sample; somatic mutation; dbSNP:rs189206655
1463 modified: Disulfide link with 1477
1477 modified: Disulfide link with 1463; C → R: in TGD; loss of interaction with APOE; unable to generate APOE-containing high density lipoproteins; moderately decreased protein abundance; moderately decreased ATPase activity; moderately decreased phospholipid translocase activity; dbSNP:rs137854494
1512 T→M: Moderately decreased protein abundance. Does not affect ATPase activity. Moderately decreased phospholipid translocase activity.
1555 I → T: in dbSNP:rs1997618
1587 K → R: associated with HDL cholesterol; dbSNP:rs2230808
1611 N → D: probable disease-associated variant; associated with atherosclerosis; deficient cellular cholesterol efflux
1615 R → Q: associated with reduced plasma HDL cholesterol; dbSNP:rs1251839800
1648 L → P: in dbSNP:rs1883024
1670 A → T: associated with reduced plasma HDL cholesterol; dbSNP:rs1203589782
1680 R → Q: associated with increased plasma HDL cholesterol; dbSNP:rs150125857
1704 V → D: in TGD; the mutant protein is retained in the endoplasmic reticulum while the wild-type protein is located at the plasma membrane
1731 S → C: in dbSNP:rs760507032
1897 R → W: in FHA1; uncertain pathological significance; dbSNP:rs760768125
1925 R → Q: in Scott syndrome; shows impaired trafficking of the mutant protein to the plasma membrane; dbSNP:rs142688906
1952 K→M: Inhibits ATPase activity; when associated with M-939. Decreases translocase activity; when associated with M-939. Does not affect protein subcellular localization in plasma membrane and endosome; when associated with M-939.
2054 modified: Phosphoserine; by PKA
2081 R → W: in TGD; highly decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; loss protein subcellular localization to the plasma membrane; dbSNP:rs137854501
2109 A → T: in a colorectal cancer sample; somatic mutation
2150 P → L: in FHA1; moderately decreased protein abundance; does not affect ATPase activity; moderately decreased phospholipid translocase activity; dbSNP:rs369098049
2163 F → S: could be associated with reduced plasma HDL cholesterol
2168 L → P: in dbSNP:rs2853577
2243 D → E: in dbSNP:rs34879708
2244 V → I: could be associated with reduced plasma HDL cholesterol; dbSNP:rs144588452

7tbyA The structure of human abca1 in nanodisc
30% identity, 98% coverage: 1:310/315 of query aligns to 680:982/1788 of 7tbyA

query
sites
7tbyA

M

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G

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V

D

D

V

P

E

Y

L

S

R

R

H

R

D

G

M

I

W

W

E

E

M

L

V

L

R

L

R

K

L

Y

R

R

A

-

T

Q

G

G

V

R

T

T

V

I

I

I

L

L

T

S

T

T

H

H

Y

H

I

M

E

D

E

E

A

A

E

D

E

V

M

L

A

G

D

D

R

R

V

I

G

A

V

I

I

I

T

S

R

H

G

G

E

K

L

L

I

C

L

C

V

V

E

G

D

S

K

S

R

L

A

F

L

L

M

K

R

N

K

Q

L

L

G

G

K

T

K

G

-

Y

Q

Y

L

L

S

T

I

L

S

V

L

K

K

V

S

S

P

A

I

I

Q

S

A

N

L

L

P

I

Q

R

G

K

L

H

D

V

E

S

Y

E

A

A

L

R

E

L

L

V

A

E

D

D

D

I

G

G

A

H

T

E

I

L

T

T

Y

Y

T

V

F

L

D

P

A

Y

Q

E

G

G

E

-

D

-

T

-

G

A

I

F

A

V

V

E

L

L

L

F

R

H

K

E

L

I

G

D

D

D

R

R

G

L

V

S

D

D

F

L

-

G

-

I

K

S

D

S

L

Y

H

G

T

I

L

S

E

E

S

T

L

L

E

E

D

E

I

I

F

F

V

L

S

K

L

V

Sites not aligning to the query:

binding cholesterol: 155, 159, 161, 162

P34358 ABC transporter ced-7; Cell death protein 7 from Caenorhabditis elegans (see 2 papers)
36% identity, 72% coverage: 12:237/315 of query aligns to 546:773/1704 of P34358

query
sites
P34358

V

I

S

I

V

V

R

R

G

N

I

L

S

V

K

K

T

I

Y

W

K

S

G

T

-

T

G

G

F

E

Q

R

A

A

L

V

K

D

S

G

I

L

D

S

L

L

D

R

I

A

R

V

R

R

G

G

E

Q

I

C

F

S

A

I

L

L

G

G

P

H

N

N

G

G

A

A

G

G

K
|
K

T

S

T

T

L

T

I

F

S

S

I

S

I

I

C

A

G

G

I

I

V

I

K

R

P

P

S

T

A

N

G

G

T

R

V

I

S

T

A

I

D

C

G

G

H

Y

D

D

V

V

L

G

R

N

D

E

Y

P

R

G

L

E

T

T

R

R

A

R

K

H

I

I

G

G

L

M

V

C

P

P

Q

Q

E

Y

L

N

S

P

T

L

D

Y

A

D

F

Q

E

L

T

T

V

V

W

S

A
x
E

A

H

V

L

R

K

F

L

S

V

R

Y

G

G

L

L

F

K

G

G

R

A

A

R

R

E

D

K

D

D

-

F

-

K

R

Q

H

D

I

M

E

K

K

R

V

L

L

L

R

S

D

D

L

V

S

K

L

L

W

D

E

F

K

K

K

E

D

N

A

E

K

K

I

A

M

V

T

N

L

L

S

S

G

G

M

M

K

K

R

R

R

K

V

L

L

C

I

V

A

C

K

M

A

A

L

L

A

I

H

G

E

D

P

S

S

E

I

V

L

V

F

L

L

L

D

D

E

E

P

P

T

T

A

A

G

G

V

M

D

D

V

P

E

G

L

A

R

R

H

Q

D

D

M

V

W

Q

E

K

M

L

V

V

R

E

R

R

L

E

R

K

A

A

T

N

G

R

V

-

T

T

V

I

I

L

L

L

T

T

H

H

Y

Y

I

M

E

D

E

E

A

A

E

E

E

R

M

L

A

G

D

D

R

W

V

V

G

F

V

I

I

M

T

S

R

H

G

G

E

K

L

L

I

V

L

A

V

S

E

G

D

T

K

N

R

Q

A

Y

L

L

M

K

R

Q

K

K

L

F

G

G

K

T

K

G

Q

Y

L

L

K586 (= K51) mutation to R: Cell corpses not engulfed.
E639 (≠ A104) mutation to G: Cell corpses not engulfed.

Sites not aligning to the query:

126 modified: carbohydrate, N-linked (GlcNAc...) asparagine
1012 modified: carbohydrate, N-linked (GlcNAc...) asparagine
1045 modified: carbohydrate, N-linked (GlcNAc...) asparagine
1417 K→R: Some cell corpses not engulfed.

P41233 Phospholipid-transporting ATPase ABCA1; ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter 1; ABC-1; ATP-binding cassette 1; EC 7.6.2.1 from Mus musculus (Mouse) (see paper)
33% identity, 74% coverage: 1:233/315 of query aligns to 888:1123/2261 of P41233

query
sites
P41233

M

M

T

E

E

E

Q

E

A

P

T

T

P

H

T

L

P

R

H

L

A

G

V

V

S

S

V

I

R

Q

G

N

I

L

S

V

K

K

T

V

Y

Y

K

R

G

D

G

G

F

M

Q

K

-

V

A

A

L

V

K

D

S

G

I

L

D

A

L

L

D

N

I

F

R

Y

R

E

G

G

E

Q

I

I

F

T

A

S

L

F

L

L

G

G

P

H

N

N

G

G

A

A

G

G

K

K

T

T

L

T

I

M

S

S

I

I

I

L

C

T

G

G

I

L

V

F

K

P

P

P

S

T

A

S

G

G

T

T

V

A

S

Y

A

I

D

L

G

G

H

K

D

D

V

I

L

R

R

S

D

E

Y

M

R

S

L

S

T

I

R

R

A

Q

K

N

I

L

G

G

L

V

V

C

P

P

Q

Q

E

H

-

N

-

V

-

L

-

F

-

D

-

M

L

L

S

T

T

V

D

E

A

-

F

-

E

E

T

H

V

I

W

W

A

F

A

Y

V

A

R

R

F

L

S

-

R

K

G

G

L

L

F

-

G

-

R

-

A

-

R

-

D

S

D

E

R

K

H

H

I

V

E

K

K

A

V

E

L

M

R

E

D

Q

L

M

S

A

L

L

-

D

-

V

-

G

-

L

-

P

W

P

E

S

K

K

K

L

D

K

A

S

K

K

I

T

M

S

T

Q

L

L

S

S

G

G

M

M

K

Q

R

R

R

K

V

L

L

S

I

V

A

A

K

L

A

A

L

F

A

V

H

G

E

G

P

S

S

K

I

V

L

V

F

I

L

L

D

D

E

E

P

P

T

T

A

A

G

G

V

V

D

D

V

P

E

Y

L

S

R

R

H

R

D

G

M

I

W

W

E

E

M

L

V

L

R

L

R

K

L

Y

R

R

A

-

T

Q

G

G

V

R

T

T

V

I

I

I

L

L

T

S

T

T

H

H

Y

H

I

M

E

D

E

E

A

A

E

D

E

I

M

L

A

G

D

D

R

R

V

I

G

A

V

I

I

I

T

S

R

H

G

G

E

K

L

L

I

C

L

C

V

V

E

G

D

S

K

S

R

L

A

F

L

L

M

K

R

N

K

Q

L

L

G

G

Sites not aligning to the query:

489 modified: carbohydrate, N-linked (GlcNAc...) asparagine

E9Q876 Glucosylceramide transporter ABCA12; ATP-binding cassette sub-family A member 12; EC 7.6.2.1 from Mus musculus (Mouse) (see 2 papers)
33% identity, 66% coverage: 11:219/315 of query aligns to 1345:1555/2595 of E9Q876

query
sites
E9Q876

A

G

V

V

S

A

V

L

R

H

G

G

I

V

S

T

K

K

T

I

Y

Y

K

-

G

G

G

S

F

K

Q

T

A

A

L

V

K

E

S

N

I

L

D

N

L

L

D

N

I

F

R

Y

R

E

G

G

E

H

I

I

F

T

A

S

L

L

G

G

P

P

N

N

G

G

A

A

G

G

K

K

T

T

L

T

I
|
I

S
|
S

I
x
M

I
x
L

C
x
T

G
|
G

I
x
L

V
x
F

K
x
G

P
x
A

S
x
T

A
|
A

G
|
G

T
|
T

V
x
I

S
x
F

A
x
V

D
x
Y

G
|
G

H
x
K

D
|
D

V
x
I

L
x
K

R
x
T

D
|
D

Y
x
L

R
x
N

L
x
T

T
x
V

R
|
R

A
x
K

K
x
N

I
x
M

G
|
G

L
x
V

V
x
C

P
x
M

Q
|
Q

E
x
H

-
x
D

-
x
V

-
x
L

-
x
F

-
x
S

-
x
Y

L
|
L

S
x
T

T
|
T

D
x
K

A
x
E

F
x
H

E
x
L

T
x
L

V
x
L

W
x
Y

A
x
G

A
x
S

V
x
I

R
x
K

F
x
V

S
x
P

R
x
H

G

-

L

-

F
x
W

G
x
T

R
x
K

A
x
T

R
x
Q

D
x
L

D
x
H

R
x
E

H
x
E

I
x
V

E
x
K

K
x
R

V

T

L

L

R

K

D

D

L

T

S

G

L

L

W

Y

E

S

K

H

K

R

D

H

A

K

K

R

I

V

M

G

T

T

L

L

S

S

G

G

M

M

K

K

R

R

R

K

V

L

L

S

I

I

A

S

K

I

A

A

L

L

A

I

H

G

E

G

P

S

S

R

I

V

L

V

F

I

L

L

D

D

E

E

P

P

T

S

A

T

G

G

V

V

D

D

V

P

E

C

L

S

R

R

H

R

D

S

M

I

W

W

E

D

M

V

V

I

R

S

R

K

L

-

R

N

A

K

T

T

G

A

V

R

T

T

V

I

I

I

L

L

T

S

T

T

H

H

Y

H

I

L

E

D

E

E

A

A

E

E

E

V

M

L

A

S

D

D

R

R

V

I

G

A

V

F

I

L

T

E

R

Q

G

G

E

G

L

L

1388:1461 (vs. 55:124, 18% identical) mutation to M: In a mouse model for harlequin ichthyosis (HI), smooth skin (smsk) mutant mice show a pronounced perinatal lethal skin phenotype in 25% of the offspring and newborn mutant pups die within a few hours after birth, and appear severely dehydrated with dry cracking skin. Smsk homozygous mutants embryos show a normal appearance at 14.5 dpc, but at 16.5 dpc develop a partial absence of normal skin folds around the trunk and limbs, and by 18.5 dpc develop a taut, thick skin and limb contractures.

Sites not aligning to the query:

1996 G→D: In a mouse model for harlequin ichthyosis (HI), homozygous mice are embryonic lethal but occasionally pups are found in the first few hours after birth but die and are severely dehydrated and fail to suckle normally. Homozygous pups show hallmarks of HI desease including hyperkeratosis, abnormal extracellular lipid lamellae and defects in cornified envelope processing. At 14.5 dpc and 15.5 dpc homozygous embryos appear normal; however from 16.5 dpc onwards they are characterized by an absence of normal skin folds around the trunk and limbs. As development progressed, embryos develop a taut, thick epidermis and multiple contractures affecting the limbs. Late stage embryos are smaller.

P78363 Retinal-specific phospholipid-transporting ATPase ABCA4; ATP-binding cassette sub-family A member 4; RIM ABC transporter; RIM proteinv; RmP; Retinal-specific ATP-binding cassette transporter; Stargardt disease protein; EC 7.6.2.1 from Homo sapiens (Human) (see 41 papers)
33% identity, 66% coverage: 11:219/315 of query aligns to 928:1138/2273 of P78363

query
sites
P78363

A
x
G

V
|
V

S
x
C

V
|
V

R
x
K

G
x
N

I
x
L

S
x
V

K
|
K

T
x
I

Y
x
F

K
x
E

G
x
P

-
x
C

G
|
G

F
x
R

Q
x
P

A
|
A

L
x
V

K
x
D

S
x
R

I
x
L

D
x
N

L
x
I

D
x
T

I
x
F

R
x
Y

R
x
E

G
x
N

E
x
Q

I
|
I

F
x
T

A
|
A

L
x
F

L
|
L

G
|
G

P
x
H

N
|
N

G
|
G

A
|
A

G
|
G

K
|
K

T
|
T

L
x
T

I
x
L

S
|
S

I
|
I

I
x
L

C
x
T

G
|
G

I
x
L

V
x
L

K
x
P

P
|
P

S
x
T

A
x
S

G
|
G

T
|
T