SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing N515DRAFT_1367 FitnessBrowser__Dyella79:N515DRAFT_1367 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

P77589 3-(3-hydroxy-phenyl)propionate transporter; 3HPP transporter; 3-(3-hydroxy-phenyl)propionate:H(+) symporter; 3HPP:H(+) symporter from Escherichia coli (strain K12) (see paper)
33% identity, 39% coverage: 63:232/437 of query aligns to 47:201/403 of P77589

query
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P77589

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D75 (= D91) mutation D->A,E: Lack of 3HPP transport activity.

Sites not aligning to the query:

27 E→A: Lack of 3HPP transport activity.; E→D: Slight decrease in 3HPP transport activity.
272 A→H: 30% increase in 3HPP transport activity.
276 K→D: Lack of 3HPP transport activity.

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
29% identity, 33% coverage: 91:234/437 of query aligns to 82:211/452 of Q5EXK5

query
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Q5EXK5

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D82 (= D91) mutation to A: Loss of activity.

Sites not aligning to the query:

311 V→W: Loss of activity.
314 D→A: Loss of activity.

Q9Y7Q9 Probable metabolite transporter C2H8.02 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
25% identity, 37% coverage: 18:179/437 of query aligns to 34:196/583 of Q9Y7Q9

query
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Q9Y7Q9

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Sites not aligning to the query:

267 modified: Phosphoserine
269 modified: Phosphoserine
289 modified: Phosphoserine
290 modified: Phosphoserine
292 modified: Phosphoserine
330 modified: Phosphoserine

Query Sequence

>N515DRAFT_1367 FitnessBrowser__Dyella79:N515DRAFT_1367
MTQAAPSVSPLDSSAPRLNRNDLRTLALAALGGALEFYDFVVFVFFTKALGQLFFPADMP
EWLAQVQVYGIFAAGYLARPLGGIVMAHFGDRTGRKRMFTLSVFLMALPTLCIGLLPTYA
QLGVFAPMLLLLLRVVQGVAIGGEVPGAWVFVAEHAPPGRVGFACASLTSGLTAGILIGS
LVAAGVTGMGAEKMLAYGWRLPFILGGVFGFFAVWLRRWLSETPVFAELRERKELASELP
LKRVMTGHLGGVLLSMLVTWMLTAAVVVVILMTPTLVQSSFHLPQKLAFHGNSLAALMLC
FGCIAGGVAVDRFGRGWSLLVGSVALLVTTYLLYRDLGQGAEHFMLLYALAGLCVGVVGV
VPAVMVAAFPPAVRFSGLSFSYNIAYAVFGAITPPLIAYLAAKLGPMAPAHYVAVTAAIG
VLCAVYLLTTRREFHER

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory