SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing N515DRAFT_1530 FitnessBrowser__Dyella79:N515DRAFT_1530 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 6 hits to proteins with known functional sites (download)

Q02I31 Putative pterin-4-alpha-carbinolamine dehydratase; PHS; 4-alpha-hydroxy-tetrahydropterin dehydratase; Pterin carbinolamine dehydratase; PCD; EC 4.2.1.96 from Pseudomonas aeruginosa (strain UCBPP-PA14) (see paper)
33% identity, 93% coverage: 6:110/113 of query aligns to 4:110/118 of Q02I31

query
sites
Q02I31

L

L

A

T

S

Q

Q

A

H

H

C

C

T

E

P

A

R

C

K

R

G

A

K

D

E

A

H

P

A

H

L

V

D

S

G

D

V

E

Q

E

I

L

E

P

T

V

L

L

A

R

R

Q

L

I

P

P

G

D

W

W

R

N

V

I

A

E

A

V

E

R

G

D

-

G

-

I

K

M

A

Q

I

L

V

E

K

K

D

V

F

Y

K

L

F

F

A

K

D

N

F

F

H

K

H

H

T

A

L

L

G

A

F

F

I

T

N

N

A

A

V

V

G

G

F

E

M

I

A

S

N

E

Q

A

E

E

D

G

H

H

P

P

D

G

L

L

E

L

A

T

G

E

Y

W

G

G

H

K

C

V

Q

T

V

V

L

T

W

W

S

W

T

S

H

H

D

S

V

I

G

K

G

G

L

L

S

H

L

R

N

N

D

D

F

F

I

I

C

M

A

A

R

R

V

T

E

D

A

E

L

V

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: Phosphothreonine

1f93A Crystal structure of a complex between the dimerization domain of hnf-1 alpha and the coactivator dcoh (see paper)
35% identity, 84% coverage: 16:110/113 of query aligns to 2:98/103 of 1f93A

query
sites
1f93A

G

G

K

K

E

A

H

H

A

R

L

L

D

S

G

A

V

E

Q

E

I

R

E

D

T

Q

L

L

A

P

R

N

L

L

P

R

-

A

-

V

G

G

W

W

R

N

V

E

A

L

A

E

E

G

G

R

K

D

A

A

I

I

V

F

K

K

D

Q

F

F

K

H

F

F

A

K

D

D

F

F

H
x
N

H
x
R

T

A

L

F

G
|
G

F

F

I

M

N

T

A
x
R

V

V

G

A

F
x
L

M

Q

A

A

N
x
E

Q

K

E

L

D

D

H
|
H

P

P

D

E

L

W

E

F

A

N

G

V

Y

Y

G

N

H

K

C

V

Q

H

V

I

L

T

W

L

S

S

T

T

H
|
H

D
x
E

V

C

G

A

G

G

L

L

S

S

L

E

N

R

D
|
D

F

I

I

N

C

L

A

A

A

S

R

F

V

I

E

E

A

Q

L

V

active site: E57 (≠ N69), H61 (= H73), H62 (= H74), H79 (= H91), E80 (≠ D92), D88 (= D100)
binding : N43 (≠ H55), R44 (≠ H56), G47 (= G59), R51 (≠ A63), L54 (≠ F66)

P61459 Pterin-4-alpha-carbinolamine dehydratase; PHS; 4-alpha-hydroxy-tetrahydropterin dehydratase; Dimerization cofactor of hepatocyte nuclear factor 1-alpha; DCoH; Dimerization cofactor of HNF1; Phenylalanine hydroxylase-stimulating protein; Pterin carbinolamine dehydratase; PCD; EC 4.2.1.96 from Rattus norvegicus (Rat) (see 2 papers)
35% identity, 84% coverage: 16:110/113 of query aligns to 3:99/104 of P61459

query
sites
P61459

G

G

K

K

E

A

H

H

A

R

L

L

D

S

G

A

V

E

Q

E

I

R

E

D

T

Q

L

L

A

P

R

N

L

L

P

R

-

A

-

V

G

G

W

W

R

N

V

E

A

L

A

E

E

G

G

R

K

D

A

A

I

I

V

F

K

K

D

Q

F

F

K

H

F

F

A

K

D

D

F

F

H

N

H

R

T

A

L

F

G

G

F

F

I

M

N

T

A

R

V

V

G

A

F

L

M

Q

A

A

N

E

Q

K

E

L

D

D

H

H

P

P

D

E

L

W

E

F

A

N

G

V

Y

Y

G

N

H

K

C

V

Q

H

V

I

L

T

W

L

S

S

T

T

H

H

D

E

V
x
C

G

A

G

G

L

L

S

S

L

E

N

R

D

D

F

I

I

N

C

L

A

A

A

S

R

F

V

I

E

E

A

Q

L

V

C82 (≠ V93) mutation to R: Reduced dehydratase activity. No impact on hydroxytetrahydrobiopterin-binding.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine

P61457 Pterin-4-alpha-carbinolamine dehydratase; PHS; 4-alpha-hydroxy-tetrahydropterin dehydratase; Dimerization cofactor of hepatocyte nuclear factor 1-alpha; DCoH; Dimerization cofactor of HNF1; Phenylalanine hydroxylase-stimulating protein; Pterin carbinolamine dehydratase; PCD; EC 4.2.1.96 from Homo sapiens (Human) (see 6 papers)
35% identity, 84% coverage: 16:110/113 of query aligns to 3:99/104 of P61457

query
sites
P61457

G

G

K

K

E

A

H

H

A

R

L

L

D

S

G

A

V

E

Q

E

I

R

E

D

T

Q

L

L

A

P

R

N

L

L

P

R

-

A

-

V

G

G

W

W

R

N

V
x
E

A
x
L

A
x
E

E
x
G

G
x
R

K
x
D

A
|
A

I
|
I

V
x
F

K
|
K

D
x
Q

F
|
F

K
x
H

F
|
F

A
x
K

D
|
D

F
|
F

H
x
N

H
x
R

T
x
A

L
x
F

G
|
G

F
|
F

I
x
M

N
x
T

A
x
R

V
|
V

G
x
A

F
x
L

M
x
Q

A
|
A

N
x
E

Q
x
K

E
x
L

D
|
D

H
|
H

P
|
P

D
x
E

L
x
W

E
x
F

A
x
N

G
x
V

Y
|
Y

G
x
N

H
x
K

C
x
V

Q
x
H

V
x
I

L
x
T

W
x
L

S
|
S

T
|
T

H
|
H

D
x
E

V
x
C

G
x
A

G
|
G

L
|
L

S
|
S

L
x
E

N
x
R

D
|
D

F
x
I

I
x
N

C
x
L

A
|
A

A
x
S

R
x
F

V
x
I

E
|
E

A
x
Q

L
x
V

T79 (= T90) to I: in HPABH4D; increased proteolytic degradation; reduced interaction with HNF1B; loss of HNF1B-coactivator activity; dbSNP:rs121913014
C82 (≠ V93) to R: in HPABH4D; increased proteolytic degradation; reduced dehydratase activity; no impact on hydroxytetrahydrobiopterin-binding; reduced interaction with HNF1B; partial impact on HNF1B-coactivator activity; dbSNP:rs104894177
R88 (≠ N99) to Q: in HPABH4D; when associated in cis with 27-E--T-104 del; uncertain significance; no impact on HNF1B interaction; no impact on HNF1B-coactivator activity; dbSNP:rs115117837
E97 (= E108) to K: in HPABH4D; increased proteolytic degradation; loss of HNF1B interaction; loss of HNF1B-coactivator activity; dbSNP:rs397518416

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
27:104 natural variant: Missing (in HPABH4D; when associated in cis with Q-88; reduced protein levels; loss of HNF1B interaction; loss of HNF1B-coactivator activity; dbSNP:rs727505360)
87:104 natural variant: Missing (in HPABH4D; increased proteolytic degradation; loss of HNF1B interaction; loss of HNF1B-coactivator activity; dbSNP:rs104894172)
98:104 natural variant: Missing (in HPABH4D; increased proteolytic degradation; loss of HNF1B interaction; loss of HNF1B-coactivator activity; dbSNP:rs121913015)

2v6tB Crystal structure of a complex of pterin-4a-carbinolamine dehydratase from toxoplasma gondii with 7,8-dihydrobiopterin (see paper)
33% identity, 75% coverage: 20:104/113 of query aligns to 5:89/100 of 2v6tB

query
sites
2v6tB

A

A

L

A

D

N

G

S

V

A

Q

R

I

L

E

L

T

Q

L

L

L

H

A

K

R

T

L

V

P

P

G

Q

W

W

R

H

V

L

A

T

A

D

E

G

G

H

K

L

A

S

I

I

V

K

K

R

D

K

F

F

K

Q

F

F

A

S

D

D

F

F

H

N

H

E

T

A

L

W

G

G

F

F

I

M

N

S

A

R

V

V

G

A

F

L

M

Y

A

A

N
x
D

Q

K

E

V

D
|
D

H
|
H

P

P

D

N

L

W

E

Y

A

N

G

V

Y

Y

G

N

H

T

C

V

Q

D

V

V

L

E

W

L

S

S

T

T

H
|
H

D
|
D

V

A

G

A

G

G

L

L

S

T

L

E

N

K

D
|
D

F

F

I

A

C

L

A

A

active site: D54 (≠ N69), H58 (= H73), H59 (= H74), H76 (= H91), D77 (= D92), D85 (= D100)
binding 2-amino-6-(1,2-dihydroxy-propyl)-7,8-dihydro-6h-pteridin-4-one: D57 (= D72), H58 (= H73), H59 (= H74), D77 (= D92)

1dcpA Dcoh, a bifunctional protein-binding transcriptional coactivator, complexed with biopterin (see paper)
34% identity, 81% coverage: 19:110/113 of query aligns to 1:94/99 of 1dcpA

query
sites
1dcpA

H

H

A

R

L

L

D

S

G

A

V

E

Q

E

I

R

E

D

T

Q

L

L

A

P

R

N

L

L

P

R

-

A

-

V

G

G

W

W

R

N

V

E

A

L

A

E

E

G

G

R

K

D

A

A

I

I

V

F

K

K

D

Q

F

F

K

H

F

F

A

K

D

D

F

F

H

N

H

R

T

A

L

F

G

G

F

F

I

M

N

T

A

R

V

V

G

A

F

L

M

Q

A

A

N
x
E

Q

K

E

L

D
|
D

H
|
H

P

P

D

E

L

W

E

F

A

N

G

V

Y

Y

G

N

H

K

C

V

Q

H

V

I

L

T

W

L

S

S

T
|
T

H
|
H

D
x
E

V

C

G

A

G

G

L

L

S

S

L

E

N

R

D
|
D

F

I

I

N

C

L

A

A

A

S

R

F

V

I

E

E

A

Q

L

V

active site: E53 (≠ N69), H57 (= H73), H58 (= H74), H75 (= H91), E76 (≠ D92), D84 (= D100)
binding 7,8-dihydrobiopterin: D56 (= D72), H57 (= H73), H58 (= H74), T74 (= T90), H75 (= H91)

Query Sequence

>N515DRAFT_1530 FitnessBrowser__Dyella79:N515DRAFT_1530
MSQADLASQHCTPRKGKEHALDGVQIETLLARLPGWRVAAEGKAIVKDFKFADFHHTLGF
INAVGFMANQEDHHPDLEAGYGHCQVLWSTHDVGGLSLNDFICAARVEALLAR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory