SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing N515DRAFT_1562 N515DRAFT_1562 sulfate transport system ATP-binding protein to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
42% identity, 79% coverage: 21:322/384 of query aligns to 36:344/378 of P69874

query
sites
P69874

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F

F45 (= F30) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ S39) mutation to T: Loss of ATPase activity and transport.
L60 (= L45) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ R61) mutation to P: Lower ATPase activity and transport efficiency.
V135 (= V124) mutation to M: Loss of ATPase activity and transport.
D172 (= D161) mutation to N: Loss of ATPase activity and transport.
C276 (≠ G261) mutation to A: Lower ATPase activity and transport efficiency.
E297 (= E279) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.

Sites not aligning to the query:

26 C→A: Lower ATPase activity and transport efficiency.
27 F→L: Lower ATPase activity and transport efficiency.

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
42% identity, 72% coverage: 10:285/384 of query aligns to 14:301/375 of 2d62A

query
sites
2d62A

R

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S

binding pyrophosphate 2-: P40 (= P36), S41 (= S37), G42 (= G38), C43 (≠ S39), G44 (= G40), K45 (= K41), T46 (≠ S42), T47 (≠ S43)

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
43% identity, 74% coverage: 1:283/384 of query aligns to 5:280/353 of 1vciA

query
sites
1vciA

M

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binding adenosine-5'-triphosphate: F16 (≠ Y12), F19 (= F15), P40 (= P36), S41 (= S37), G42 (= G38), C43 (≠ S39), G44 (= G40), K45 (= K41), T46 (≠ S42), T47 (≠ S43)

1g291 Malk (see paper)
45% identity, 70% coverage: 12:281/384 of query aligns to 13:294/372 of 1g291

query
sites
1g291

Y

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D

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D

L

L

binding magnesium ion: D69 (= D62), E71 (≠ T64), K72 (≠ D65), K79 (≠ Q72), D80 (≠ R73), Y228 (= Y225), D229 (≠ R226), E292 (= E279), D293 (≠ H280)
binding pyrophosphate 2-: P37 (= P36), S38 (= S37), G39 (= G38), C40 (≠ S39), G41 (= G40), K42 (= K41), T43 (≠ S42), T44 (≠ S43)

Sites not aligning to the query:

binding magnesium ion: 341, 342, 359

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
36% identity, 94% coverage: 2:362/384 of query aligns to 3:367/369 of P19566

query
sites
P19566

S

S

L

V

S

Q

I

L

R

R

Q

N

L

V

T

T

R

K

R

A

Y

W

G

G

A

D

F

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V

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S

D

K

D

D

F

I

S

N

L

L

D

D

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A

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E

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G

G

E

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F

F

V

V

A

V

L

F

L

V

G

G

P

P

S

S

G

G

S

C

G

G

K

K

S

S

S

T

L

L

R

R

I

M

L

I

A

A

G

G

L

L

D

E

D

T

P

I

D

T

R

S

G

G

D

D

V

L

L

F

R

I

D

G

G

E

T

T

D

R

L

M

L

N

A

D

L

I

P

P

A

P

Q

A

R

E

R

R

D

G

I

V

G

G

L

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L
|
L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

D

E

N

N

I

M

A

S

F

F

G

G

L

L

R

K

V

L

R

A

P

-

R

-

A

-

R

-

R

G

P

A

S

K

R

K

R

E

D

V

I

M

A

N

A

Q

R

R

V

V

E

N

D

Q

L

V

L

A

R

E

R

V

V

L

Q

Q

L

L

E

A

E

H

L

L

G

L

R

E

R

R

Y

K

P

P

T

K

Q

A

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

V

A

E

E

P

P

S

R

L

V

L

F

L

L

D

D

E

E

P
|
P

F

L

G

S

A

N

L

L

D
|
D

A

A

Q

A

V

L

R

R

G

V

T

Q

L

M

R

R

V

I

W

E

L

I

R

S

D

R

L

L

Q

H

R

K

S

R

L

L

G

G

L

R

T

T

T

M

V

I

L

Y

V

V

T

T

H

H

D

D

Q

Q

D

V

E

E

A

A

L

M

E

T

L

L

A

A

D

D

R

K

V

I

V

V

M

L

N

D

R

A

G

G

R

R

I

V

E

A

Q

Q

V

V

G

G

A

K

P

P

S

L

E

E

I

L

Y

Y

R

H

E

Y

P

P

A

A

T

D

P

R

F

F

V

V

H

A

G

G

F

F

V

I

G

G

-

S

-

P

R

K

A

M

N

N

R

F

I

L

R

P

G

V

H

K

V

V

E

T

R

A

D

T

R

A

L

I

H

-

L

-

G

-

H

E

S

Q

F

V

Q

Q

G

V

E

E

L

L

P

P

-

N

-

R

-

Q

-

I

-

W

-

L

-

P

-

V

-

E

-

S

-

R

G

G

D

V

L

Q

A

V

G

G

R

A

E

N

I

M

E

S

A

L

W

G

L

I

R

R

P

P

E

E

H

H

L

L

A

L

L

P

A

S

S

D

R

I

G

A

L

D

G

V

G

T

W

L

T

E

G

G

R

E

L

V

Q

Q

H

V

L

V

D
x
E

L

Q

A

L

G

G

-

H

-

E

-

T

-

Q

-

I

-

H

-

I

-

Q

-

I

P

P

V

A

A

I

R

R

A

Q

R

N

L

L

A

V

M

Y

H

R

G

Q

D

N

G

D

L

V

V

V

L

L

D

V

A

E

E

E

W

G

N

A

A

T

A

-

E

-

V

-

A

-

H

-

G

-

L

F

A

A

I

I

G

G

E

-

V

-

T

-

L

L

Q

P

P

P

R

E

E

R

F

C

T

H

L

L

F

F

A

R

D

E

V

D

P

G

G

S

G

A

V

C

R

R

L

L

R

H

F

Q

V

E

P

P

L86 (= L85) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P163) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D168) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
E306 (≠ D299) mutation to K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

2awoA Crystal structure of the adp-mg-bound e. Coli malk (crystallized with adp-mg) (see paper)
46% identity, 62% coverage: 2:238/384 of query aligns to 2:234/372 of 2awoA

query
sites
2awoA

S

S

L

V

S

Q

I

L

R

Q

Q

N

L

V

T

T

R

K

R

A

Y
x
W

G

G

A

E

F

V

A

V

A
x
V

L

S

D

K

D

D

F

I

S

N

L

L

D

D

I

I

A

H

E

E

G

G

E

E

F

F

V

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

S
x
T

L

L

R

R

I

M

L

I

A

A

G

G

L

L

D

E

D

T

P

I

D

T

R

S

G

G

D

D

V

L

L

F

R

I

D

G

G

E

T

K

D

R

L

M

L

N

A

D

L

T

P

P

A

P

Q

A

R

E

R

R

D

G

I

V

G

G

L

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

D

E

N

N

I

M

A

S

F

F

G

G

L

L

R

K

V

L

R

A

P

-

R

-

A

-

R

-

R

G

P

A

S

K

R

K

R

E

D

V

I

I

A

N

A

Q

R

R

V

V

E

N

D

Q

L

V

L

A

R

E

R

V

V

L

Q

Q

L

L

E

A

E

H

L

L

G

L

R

D

R

R

Y

K

P

P

T

K

Q

A

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

V

A

E

E

P

P

S

S

L

V

L

F

L

L

D
|
D

E

E

P

P

F

L

G

S

A

N

L

L

D

D

A

A

Q

A

V

L

R

R

G

V

T

Q

L

M

R

R

V

I

W

E

L

I

R

S

D

R

L

L

Q

H

R

K

S

R

L

L

G

G

L

R

T

T

T

M

V

I

L

Y

V

V

T

T

H

H

D

D

Q

Q

D

V

E

E

A

A

L

M

E

T

L

L

A

A

D

D

R

K

V

I

V

V

M

L

N

D

R

A

G

G

R

R

I

V

E

A

Q

Q

V

V

G

G

A

K

P

P

S

L

E

E

I

L

Y

Y

R

H

E

Y

P

P

A

A

T

D

P

R

F

F

V

V

H

A

G

G

F

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ Y12), V17 (≠ A17), S37 (= S37), G38 (= G38), C39 (≠ S39), G40 (= G40), K41 (= K41), S42 (= S42), T43 (≠ S43)
binding magnesium ion: S42 (= S42), D157 (= D161)

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
46% identity, 62% coverage: 2:238/384 of query aligns to 2:234/371 of 3puyA

query
sites
3puyA

S

S

L

V

S

Q

I

L

R

Q

Q

N

L

V

T

T

R

K

R

A

Y
x
W

G

G

A

E

F

V

A

V

A
x
V

L

S

D

K

D

D

F

I

S

N

L

L

D

D

I

I

A

H

E

E

G

G

E

E

F

F

V

V

A

V

L

F

L

V

G

G

P
|
P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

S
x
T

L

L

R

R

I

M

L

I

A

A

G

G

L

L

D

E

D

T

P

I

D

T

R

S

G

G

D

D

V

L

L

F

R

I

D

G

G

E

T

K

D

R

L

M

L

N

A

D

L

T

P

P

A

P

Q

A

R

E

R

R

D

G

I

V

G

G

L

M

V

V

F

F

Q
|
Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

D

E

N

N

I

M

A

S

F

F

G

G

L

L

R

K

V

L

R

A

P

-

R

-

A

-

R

-

R

G

P

A

S

K

R

K

R

E

D

V

I

I

A

N

A

Q

R

R

V

V

E

N

D

Q

L

V

L

A

R

E

R

V

V

L

Q

Q

L

L

E

A

E

H

L

L

G

L

R

D

R
|
R

Y

K

P

P

T

K

Q
x
A

L

L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

V

A

E

E

P

P

S

S

L

V

L

F

L

L

D
|
D

E
|
E

P

P

F

L

G

S

A

N

L

L

D

D

A

A

Q

A

V

L

R

R

G

V

T

Q

L

M

R

R

V

I

W

E

L

I

R

S

D

R

L

L

Q

H

R

K

S

R

L

L

G

G

L

R

T

T

T

M

V

I

L

Y

V

V

T

T

H
|
H

D

D

Q

Q

D

V

E

E

A

A

L

M

E

T

L

L

A

A

D

D

R

K

V

I

V

V

M

L

N

D

R

A

G

G

R

R

I

V

E

A

Q

Q

V

V

G

G

A

K

P

P

S

L

E

E

I

L

Y

Y

R

H

E

Y

P

P

A

A

T

D

P

R

F

F

V

V

H

A

G

G

F

F

V

I

G

G

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y12), V17 (≠ A17), P36 (= P36), S37 (= S37), G38 (= G38), C39 (≠ S39), G40 (= G40), K41 (= K41), S42 (= S42), T43 (≠ S43), Q81 (= Q81), R128 (= R132), A132 (≠ Q136), S134 (= S138), G135 (= G139), G136 (= G140), Q137 (= Q141), E158 (= E162), H191 (= H195)
binding magnesium ion: S42 (= S42), Q81 (= Q81), D157 (= D161)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
46% identity, 62% coverage: 2:238/384 of query aligns to 2:234/371 of 3puxA

query
sites
3puxA

S

S

L

V

S

Q

I

L

R

Q

Q

N

L

V

T

T

R

K

R

A

Y
x
W

G

G

A

E

F

V

A

V

A
x
V

L

S

D

K

D

D

F

I

S

N

L

L

D

D

I

I

A

H

E

E

G

G

E

E

F

F

V

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

S
x
T

L

L

R

R

I

M

L

I

A

A

G

G

L

L

D

E

D

T

P

I

D

T

R

S

G

G

D

D

V

L

L

F

R

I

D

G

G

E

T

K

D

R

L

M

L

N

A

D

L

T

P

P

A

P

Q

A

R

E

R

R

D

G

I

V

G

G

L

M

V

V

F

F

Q
|
Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

D

E

N

N

I

M

A

S

F

F

G

G

L

L

R

K

V

L

R

A

P

-

R

-

A

-

R

-

R

G

P

A

S

K

R

K

R

E

D

V

I

I

A

N

A

Q

R

R

V

V

E

N

D

Q

L

V

L

A

R

E

R

V

V

L

Q

Q

L

L

E

A

E

H

L

L

G

L

R

D

R
|
R

Y

K

P

P

T

K

Q
x
A

L
|
L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

V

A

E

E

P

P

S

S

L

V

L

F

L

L

D
|
D

E
|
E

P

P

F

L

G

S

A

N

L

L

D

D

A

A

Q

A

V

L

R

R

G

V

T

Q

L

M

R

R

V

I

W

E

L

I

R

S

D

R

L

L

Q

H

R

K

S

R

L

L

G

G

L

R

T

T

T

M

V

I

L

Y

V

V

T

T

H
|
H

D

D

Q

Q

D

V

E

E

A

A

L

M

E

T

L

L

A

A

D

D

R

K

V

I

V

V

M

L

N

D

R

A

G

G

R

R

I

V

E

A

Q

Q

V

V

G

G

A

K

P

P

S

L

E

E

I

L

Y

Y

R

H

E

Y

P

P

A

A

T

D

P

R

F

F

V

V

H

A

G

G

F

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ Y12), V17 (≠ A17), S37 (= S37), G38 (= G38), C39 (≠ S39), G40 (= G40), K41 (= K41), S42 (= S42), T43 (≠ S43), R128 (= R132), A132 (≠ Q136), L133 (= L137), S134 (= S138), Q137 (= Q141)
binding beryllium trifluoride ion: S37 (= S37), G38 (= G38), K41 (= K41), Q81 (= Q81), S134 (= S138), G135 (= G139), G136 (= G140), E158 (= E162), H191 (= H195)
binding magnesium ion: S42 (= S42), Q81 (= Q81), D157 (= D161)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
46% identity, 62% coverage: 2:238/384 of query aligns to 2:234/371 of 3puwA

query
sites
3puwA

S

S

L

V

S

Q

I

L

R

Q

Q

N

L

V

T

T

R

K

R

A

Y
x
W

G

G

A

E

F

V

A

V

A
x
V

L

S

D

K

D

D

F

I

S

N

L

L

D

D

I

I

A

H

E

E

G

G

E

E

F

F

V

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

S
x
T

L

L

R

R

I

M

L

I

A

A

G

G

L

L

D

E

D

T

P

I

D

T

R

S

G

G

D

D

V

L

L

F

R

I

D

G

G

E

T

K

D

R

L

M

L

N

A

D

L

T

P

P

A

P

Q

A

R

E

R

R

D

G

I

V

G

G

L

M

V

V

F

F

Q
|
Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

D

E

N

N

I

M

A

S

F

F

G

G

L

L

R

K

V

L

R

A

P

-

R

-

A

-

R

-

R

G

P

A

S

K

R

K

R

E

D

V

I

I

A

N

A

Q

R

R

V

V

E

N

D

Q

L

V

L

A

R

E

R

V

V

L

Q

Q

L

L

E

A

E

H

L

L

G

L

R

D

R
|
R

Y

K

P

P

T

K

Q
x
A

L

L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

V

A

E

E

P

P

S

S

L

V

L

F

L

L

D

D

E
|
E

P

P

F

L

G

S

A
x
N

L

L

D

D

A

A

Q

A

V

L

R

R

G

V

T

Q

L

M

R

R

V

I

W

E

L

I

R

S

D

R

L

L

Q

H

R

K

S

R

L

L

G

G

L

R

T

T

T

M

V

I

L

Y

V

V

T

T

H
|
H

D

D

Q

Q

D

V

E

E

A

A

L

M

E

T

L

L

A

A

D

D

R

K

V

I

V

V

M

L

N

D

R

A

G

G

R

R

I

V

E

A

Q

Q

V

V

G

G

A

K

P

P

S

L

E

E

I

L

Y

Y

R

H

E

Y

P

P

A

A

T

D

P

R

F

F

V

V

H

A

G

G

F

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ Y12), V17 (≠ A17), S37 (= S37), G38 (= G38), C39 (≠ S39), G40 (= G40), K41 (= K41), S42 (= S42), T43 (≠ S43), R128 (= R132), A132 (≠ Q136), S134 (= S138), Q137 (= Q141)
binding tetrafluoroaluminate ion: S37 (= S37), G38 (= G38), K41 (= K41), Q81 (= Q81), S134 (= S138), G135 (= G139), G136 (= G140), E158 (= E162), N162 (≠ A166), H191 (= H195)
binding magnesium ion: S42 (= S42), Q81 (= Q81)

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
46% identity, 62% coverage: 2:238/384 of query aligns to 2:234/374 of 2awnB

query
sites
2awnB

S

S

L

V

S

Q

I

L

R

Q

Q

N

L

V

T

T

R

K

R

A

Y
x
W

G

G

A

E

F

V

A

V

A
x
V

L

S

D

K

D

D

F

I

S

N

L

L

D

D

I

I

A

H

E

E

G

G

E

E

F

F

V

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

S
x
T

L

L

R

R

I

M

L

I

A

A

G

G

L

L

D

E

D

T

P

I

D

T

R

S

G

G

D

D

V

L

L

F

R

I

D

G

G

E

T

K

D

R

L

M

L

N

A

D

L

T

P

P

A

P

Q

A

R

E

R

R

D

G

I

V

G

G

L

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

D

E

N

N

I

M

A

S

F

F

G

G

L

L

R

K

V

L

R

A

P

-

R

-

A

-

R

-

R

G

P

A

S

K

R

K

R

E

D

V

I

I

A

N

A

Q

R

R

V

V

E

N

D

Q

L

V

L

A

R

E

R

V

V

L

Q

Q

L

L

E

A

E

H

L

L

G

L

R

D

R

R

Y

K

P

P

T

K

Q

A

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

V

A

E

E

P

P

S

S

L

V

L

F

L

L

D

D

E

E

P

P

F

L

G

S

A

N

L

L

D

D

A

A

Q

A

V

L

R

R

G

V

T

Q

L

M

R

R

V

I

W

E

L

I

R

S

D

R

L

L

Q

H

R

K

S

R

L

L

G

G

L

R

T

T

T

M

V

I

L

Y

V

V

T

T

H

H

D

D

Q

Q

D

V

E

E

A

A

L

M

E

T

L

L

A

A

D

D

R

K

V

I

V

V

M

L

N

D

R

A

G

G

R

R

I

V

E

A

Q

Q

V

V

G

G

A

K

P

P

S

L

E

E

I

L

Y

Y

R

H

E

Y

P

P

A

A

T

D

P

R

F

F

V

V

H

A

G

G

F

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ Y12), V17 (≠ A17), S37 (= S37), G38 (= G38), C39 (≠ S39), G40 (= G40), K41 (= K41), S42 (= S42), T43 (≠ S43)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
46% identity, 62% coverage: 2:238/384 of query aligns to 3:235/371 of P68187

query
sites
P68187

S

S

L

V

S

Q

I

L

R

Q

Q

N

L

V

T

T

R

K

R

A

Y

W

G

G

A

E

F

V

A

V

L

S

D

K

D

D

F

I

S

N

L

L

D

D

I

I

A

H

E

E

G

G

E

E

F

F

V

V

A

V

L

F

L

V

G

G

P

P

S

S

G

G

S

C

G

G

K

K

S

S

S

T

L

L

R

R

I

M

L

I

A

A

G

G

L

L

D

E

D

T

P

I

D

T

R

S

G

G

D

D

V

L

L

F

R

I

D

G

G

E

T

K

D

R

L

M

L

N

A

D

L

T

P

P

A

P

Q

A

R

E

R

R

D

G

I

V

G

G

L

M

V

V

F

F

Q

Q

H

S

Y

Y

A
|
A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

D

E

N

N

I

M

A

S

F

F

G

G

L

L

R

K

V

L

R

A

P

-

R

-

A

-

R

-

R

G

P

A

S
x
K

R

K

R

E

D

V

I

I

A

N

A

Q

R

R

V
|
V

E

N

D

Q

L
x
V

L

A

R
x
E

R

V

V

L

Q

Q

L

L

E
x
A

E

H

L

L

G

L

R

D

R

R

Y

K

P

P

T

K

Q

A

L

L

S

S

G

G

G
|
G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

V

A

E

E

P

P

S

S

L

V

L

F

L

L

D
|
D

E

E

P

P

F

L

G

S

A

N

L

L

D

D

A

A

Q

A

V

L

R

R

G

V

T

Q

L

M

R

R

V

I

W

E

L

I

R

S

D

R

L

L

Q

H

R

K

S

R

L

L

G

G

L

R

T

T

T

M

V

I

L

Y

V

V

T

T

H

H

D

D

Q

Q

D

V

E

E

A

A

L

M

E

T

L

L

A

A

D

D

R

K

V

I

V

V

M

L

N

D

R

A

G

G

R

R

I

V

E

A

Q

Q

V

V

G

G

A

K

P

P

S

L

E

E

I

L

Y

Y

R

H

E

Y

P

P

A

A

T

D

P
x
R

F

F

V

V

H

A

G

G

F

F

V

I

G

G

A85 (= A84) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ S109) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V117) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ L120) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ R122) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ E127) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G140) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D161) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ P231) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

7cagC Mycobacterium smegmatis lpqy-sugabc complex in the catalytic intermediate state (see paper)
48% identity, 60% coverage: 7:238/384 of query aligns to 7:232/362 of 7cagC

query
sites
7cagC

Q

R

L

V

T

T

R

K

R

S

Y

Y

G

P

A
|
A

F

-

A

-

A

A

L

V

D

K

D

E

F

F

S

S

L

M

D

T

I

I

A

A

E

D

G

G

E

E

F

F

V

I

A

I

L

L

L

V

G

G

P
|
P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

S
x
T

L

T

L

L

R

N

I

M

L

I

A

A

G

G

L

L

D

E

P

I

D

T

R

S

G

G

D

E

V

L

L

R

R

I

D

G

G

G

T

E

D

R

L

V

L

N

A

E

L

K

P

A

A

P

Q

K

R

D

R

R

D

D

I

I

G

A

L

M

V

V

F

F

Q
|
Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

V

V

A

R

D

Q

N

N

I

I

A

A

F

F

G

P

L

L

R

T

V

L

R

-

P

-

R

-

A

A

R

K

R

V

P

P

S

-

R

K

R

A

D

E

I

I

A

A

R

K

V

V

E

E

D

E

L

T

L

A

R

K

R

I

V

L

Q

D

L

L

E

S

E

E

L
|
L

G

L

R

D

R
|
R

Y

K

P

P

T

G

Q
|
Q

L

L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

A

V

V

R

E

S

P

P

S

K

L

A

L

F

L

L

L

M

D

D

E

Q

P

P

F

L

G

S

A

N

L

L

D

D

A

A

Q

K

V

L

R

R

G

V

T

Q

L

M

R

R

V

A

W

E

L

I

R

S

D

R

L

L

Q

Q

R

D

S

R

L

L

G

G

L

T

T

T

V

V

L

Y

V

V

T

T

H

H

D

D

Q

Q

D

T

E

E

A

A

L

M

E

T

L

L

A

G

D

D

R

R

V

V

M

M

N

L

R

A

G

G

R

E

I

V

E

Q

Q

Q

V

I

G

G

A

T

P

P

S

D

E

E

I

L

Y

Y

R

S

E

S

P

P

A

A

T

N

P

L

F

F

V

V

H

A

G

G

F

F

V

I

G

G

binding adenosine-5'-triphosphate: A14 (= A14), P34 (= P36), S35 (= S37), G36 (= G38), C37 (≠ S39), G38 (= G40), K39 (= K41), S40 (= S42), T41 (≠ S43), Q79 (= Q81), L123 (= L129), R126 (= R132), Q130 (= Q136), S132 (= S138), G133 (= G139), G134 (= G140), Q135 (= Q141)
binding magnesium ion: S40 (= S42), Q79 (= Q81)

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
45% identity, 61% coverage: 3:238/384 of query aligns to 1:232/367 of 1q12A

query
sites
1q12A

L

V

S

Q

I

L

R

Q

Q

N

L

V

T

T

R

K

R

A

Y
x
W

G

G

A

E

F

V

A

V

A
x
V

L

S

D

K

D

D

F

I

S

N

L

L

D

D

I

I

A

H

E

E

G

G

E

E

F

F

V

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

S
x
T

L

L

R

R

I

M

L

I

A

A

G

G

L

L

D

E

D

T

P

I

D

T

R

S

G

G

D

D

V

L

L

F

R

I

D

G

G

E

T

K

D

R

L

M

L

N

A

D

L

T

P

P

A

P

Q

A

R

E

R

R

D

G

I

V

G

G

L

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

A

A

D

E

N

N

I

M

A

S

F

F

G

G

L

L

R

K

V

L

R

A

P

-

R

-

A

-

R

-

R

G

P

A

S

K

R

K

R

E

D

V

I

I

A

N

A

Q

R

R

V

V

E

N

D

Q

L

V

L

A

R

E

R

V

V

L

Q

Q

L

L

E

A

E

H

L

L

G

L

R

D

R
|
R

Y

K

P

P

T
x
K

Q
x
A

L
|
L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

A

V

V

A

E

E

P

P

S

S

L

V

L

F

L

L

D

D

E

E

P

P

F

L

G

S

A
x
N

L

L

D

D

A

A

Q

A

V

L

R

R

G

V

T

Q

L

M

R

R

V

I

W

E

L

I

R

S

D

R

L

L

Q

H

R

K

S

R

L

L

G

G

L

R

T

T

T

M

V

I

L

Y

V

V

T

T

H
|
H

D

D

Q

Q

D

V

E

E

A

A

L

M

E

T

L

L

A

A

D

D

R

K

V

I

V

V

M

L

N

D

R

A

G

G

R

R

I

V

E

A

Q

Q

V

V

G

G

A

K

P

P

S

L

E

E

I

L

Y

Y

R

H

E

Y

P

P

A

A

T

D

P

R

F

F

V

V

H

A

G

G

F

F

V

I

G

G

binding adenosine-5'-triphosphate: W10 (≠ Y12), V15 (≠ A17), S35 (= S37), G36 (= G38), C37 (≠ S39), G38 (= G40), K39 (= K41), S40 (= S42), T41 (≠ S43), R126 (= R132), K129 (≠ T135), A130 (≠ Q136), L131 (= L137), S132 (= S138), G133 (= G139), G134 (= G140), Q135 (= Q141), N160 (≠ A166), H189 (= H195)

3d31A Modbc from methanosarcina acetivorans (see paper)
40% identity, 74% coverage: 3:285/384 of query aligns to 2:280/348 of 3d31A

query
sites
3d31A

L

I

S

E

I

I

R

E

Q

S

L

L

T

S

R

R

R

K

Y

W

G

K

A

N

F

F

A

S

A

-

L

L

D

D

D

N

F

L

S

S

L

L

D

K

I

V

A

E

E

S

G

G

E

E

F

Y

V

F

A

V

L

I

L

L

G

G

P

P

S

T

G

G

S

A

G

G

K

K

S

T

S

L

L

F

L

L

R

E

I

L

L

I

A

A

G

G

L

F

D

H

D

V

P

P

D

D

R

S

G

G

D

R

V

I

L

L

R

L

D

D

G

G

T

K

D

D

L

V

L

T

A

D

L

L

P

S

A

P

Q

E

R

K

R

H

D

D

I

I

G

A

L

F

V

V

F

Y

Q

Q

H

N

Y

Y

A

S

L

L

F

F

P

P

H

H

M

M

T

N

V

V

A

K

D

K

N

N

I

L

A

E

F

F

G

G

L

M

R

R

V

M

R

K

P

-

R

K

A

I

R

K

R

D

P

P

S

K

R

R

R

-

D

-

I

-

A

-

R

-

V

V

E

L

D

D

L

T

L

A

R

R

R

D

V

L

Q

K

L

I

E

E

E

H

L

L

G

L

R

D

R

R

Y

N

P

P

T

L

Q

T

L

L

S

S

G

G

Q

E

R

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

A

V

V

T

E

N

P

P

S

K

L

I

L

L

D

D

E

E

P

P

F

L

G

S

A

A

L

L

D

D

A

P

Q

R

V

T

R

Q

G

E

T

N

L

A

R

R

V

E

W

M

L

L

R

S

D

V

L

L

Q

H

R

K

S

K

L

N

G

K

L

L

T

T

T

V

V

L

L

H

V

I

T

T

H

H

D

D

Q

Q

D

T

E

E

A

A

L

R

E

I

L

M

A

A

D

D

R

R

V

I

V

A

V

V

M

V

N

M

R

D

G

G

R

K

I

L

E

I

Q

Q

V

V

G

G

A

K

P

P

S

E

E

E

I

I

Y

F

R

E

E

K

P

P

A

V

T

E

P

G

F

R

V

V

H

A

G

S

F

F

V

V

G

G

R

F

A

E

N

N

R

V

I

L

R

K

G

G

H

R

V

V

-

I

-

S

-

A

E

E

R

Q

D

G

R

L

L

L

H

R

L

I

G

R

G

V

H

G

S

E

F

V

Q

V

G

I

E

D

L

A

P

A

G

G

D

D

L

M

-

E

A

V

G

G

R

D

E

Q

I

V

E

Y

A

A

W

F

L

L

R

R

P

P

E

E

H

N

L

I

A

A

L

L

A

S

S

K

Sites not aligning to the query:

binding tungstate(vi)ion: 286, 287, 288, 319, 320, 323, 340, 341, 342

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
48% identity, 58% coverage: 16:238/384 of query aligns to 18:236/393 of P9WQI3

query
sites
P9WQI3

A

T

A

A

L

V

D

R

D

D

F

L

S

N

L

L

D

T

I

I

A

A

E

D

G

G

E

E

F

F

V

L

A

I

L

L

L

V

G

G

P

P

S

S

G

G

S

C

G

G

K

K

S

T

L

T

L

L

R

N

I

M

L

I

A

A

G

G

L

L

D

E

D

D

P

I

D

S

R

S

G

G

D

E

V

L

L

R

R

I

D

A

G

G

T

E

D

R

L

V

L

N

A

E

L

K

P

A

A

P

Q

K

R

D

R

R

D

D

I

I

G

A

L

M

V

V

F

F

Q

Q

H

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

V

V

A

R

D

Q

N

N

I

I

A

A

F

F

G

P

L

L

R

T

V

L

R

A

P

-

R

-

A

-

R

-

R

K

P

M

S

R

R

K

R

A

D

D

I

I

A

A

A

Q

R

K

V

V

E

S

D

E

L

T

L

A

R

K

R

I

V

L

Q

D

L

L

E

T

E

N

L

L

G

L

R

D

R

R

Y

K

P

P

T

S

Q

Q

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

A

V

V

R

E

H

P

P

S

K

L

A

L

F

L

L

L

M

D

D

E

E

P

P

F

L

G

S

A

N

L

L

D

D

A

A

Q

K

V

L

R

R

G

V

T

Q

L

M

R

R

V

G

W

E

L

I

R

A

D

Q

L

L

Q

Q

R

R

S

R

L

L

G

G

L

T

T

T

V

V

L

Y

V

V

T

T

H
|
H

D

D

Q

Q

D

T

E

E

A

A

L

M

E

T

L

L

A

G

D

D

R

R

V

V

M

M

N

Y

R

G

G

G

R

I

I

A

E

Q

Q

Q

V

I

G

G

A

T

P

P

S

E

E

E

I

L

Y

Y

R

E

E

R

P

P

A

A

T

N

P

L

F

F

V

V

H

A

G

G

F

F

V

I

G

G

H193 (= H195) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
34% identity, 87% coverage: 11:344/384 of query aligns to 14:344/353 of 1oxvD

query
sites
1oxvD

R

K

Y

K

G

G

A

K

F
x
V

A

V

A
|
A

L

L

D

D

D

N

F

V

S

N

L

I

D

N

I

I

A

E

E

N

G

G

E

E

F

R

V

F

A

G

L

I

L

L

G

G

P
|
P

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
x
T

L

F

L

M

R

R

I

I

L

I

A

A

G

G

L

L

D

D

D

V

P

P

D

S

R

T

G

G

D

E

V

L

L

Y

R

F

D

D

-

D

-

R

-

L

-

V

-

A

G

S

T

N

D

G

L

K

L

L

A

I

L

V

P

P

A

P

Q

E

R

D

R

R

D

K

I

I

G

G

L

M

V

V

F

F

Q
|
Q

H

T

Y

W

A

A

L

L

F

Y

P

P

H

N

M

L

T

T

V

A

A

F

D

E

N

N

I

I

A

A

F

F

G

P

L

L

R

T

V

-

R

-

P

-

R

-

A

N

R

M

R

K

P

M

S

S

R

K

R

E

D

E

I

I

A

R

A

K

R

R

V

V

E

E

D

E

L

V

L

A

R

K

R

I

V

L

Q

D

L

I

E

H

L

V

G

L

R

N

R

H

Y

F

P

P

T

R

Q

E

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

A

V

V

K

E

D

P

P

S

S

L

L

D

D

E
|
E

P

P

F

F

G

S

A

N

L

L

D

D

A

A

Q

R

V

M

R

R

G

D

T

S

L

A

R

R

V

A

W

L

L

V

R

K

D

E

L

V

Q

Q

R

S

S

R

L

L

G

G

L

V

T

T

T

L

V

L

L

V

V

V

T

S

H

H

D

D

Q

P

D

A

E

D

A

I

L

F

E

A

L

I

A

A

D

D

R

R

V

V

V

G

V

V

M

L

N

V

R

K

G

G

R

K

I

L

E

V

Q

Q

V

V

G

G

A

K

P

P

S

E

E

D

I

L

Y

Y

R

D

E

N

P

P

A

V

T

S

P

I

F

Q

V

V

H

A

G

S

F

L

V

I

G

G

R

E

A

I

N

N

R

E

I

L

R

E

G

G

H

K

V

V

E

T

R

N

D

E

R

G

L

V

H

V

L

I

G

G

G

S

H

L

S

R

F

F

Q

P

G

V

E

S

L

V

P

S

G

S

D

D

L

R

A

A

G

-

R

-

E

-

I

-

E

I

A

I

W

G

L

I

R

R

P

P

E

E

H

D

L

V

A

K

L

L

A

S

S

K

R

D

G

V

L

I

G

K

-

D

G

S

W

W

T

I

-

L

-

V

-

G

-

K

G

G

R

K

L

V

Q

K

H

V

L

I

D

G

L

Y

A

Q

G

G

P

G

V

L

A

F

R

R

A

I

R

T

L

I

A

T

M

P

H

-

G

-

D

-

G

-

L

-

V

-

L

L

D

D

A

S

E

E

W

E

N

E

A

I

A

F

E

T

V

Y

A

S

A

D

H

H

G

P

L

I

A

H

I

S

G

G

E

E

V

E

V

V

T

L

L

V

Q

Y

P

V

R

R

E

K

binding phosphoaminophosphonic acid-adenylate ester: V18 (≠ F15), A20 (= A17), P39 (= P36), S40 (= S37), G41 (= G38), A42 (≠ S39), G43 (= G40), K44 (= K41), T45 (≠ S42), T46 (≠ S43), Q89 (= Q81), E166 (= E162)
binding magnesium ion: T45 (≠ S42), Q89 (= Q81)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13

1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
34% identity, 87% coverage: 11:344/384 of query aligns to 14:344/353 of 1oxvA

query
sites
1oxvA

R

K

Y

K

G

G

A

K

F
x
V

A

V

A
|
A

L

L

D

D

D

N

F

V

S

N

L

I

D

N

I

I

A

E

E

N

G

G

E

E

F

R

V

F

A

G

L

I

L

L

G

G

P
|
P

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
x
T

L

F

L

M