SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing N515DRAFT_2015 N515DRAFT_2015 branched-chain amino acid aminotransferase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
58% identity, 98% coverage: 7:305/305 of query aligns to 3:303/304 of 1iyeA

query
sites
1iyeA

E

D

W

Y

I

I

W

W

Q

F

N

N

G

G

Q

E

I

M

K

V

P

R

W

W

R

E

E

D

A

A

T

K

T

V

H

H

V

V

M

M

S

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

S

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

S

C

Y

Y

A

D

T

S

P

H

D

K

G

G

A

P

A

V

I

V

F

F

R

R

L

H

T

R

D

E

H

H

L

M

K

Q

R
|
R

L

L

Y

H

Q

D

S

S

A

A

K

K

I

I

Y

Y

D

R

M

F

V

P

L

V

P

S

Y

Q

S

S

Q

I

D

D

Q

E

I

L

A

M

A

E

A

A

C

C

R

R

D

D

V

V

I

I

K

R

Q

K

N

N

G

N

L

L

G

T

A

S

A

A

Y
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Y

L

I

R
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R

P

P

V

L

A

I

Y

F

R

V

G

G

L

D

G

V

G

G

F

M

G

G

L

V

S

N

-

P

A

A

E

G

T

Y

P

S

I

T

D

D

V

V

A

I

V

I

A

A

W

F

P

P

M

W

G

G

P

A

Y
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Y

L

L

G

G

P

A

E

E

A

A

L

L

E

E

S

Q

G

G

I

I

D

D

A

A

C

M

V

V

S

S

W

W

Q

N

R

R

F

A

A

A

P

P

N

N

T

T

I

I

P

P

A

T

G

A

A

A

K
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K

A
|
A

G

G

N

N

Y
|
Y

L

L

S

S

G

S

Q

L

L

L

I

V

A

G

R

S

E

E

A

A

R

R

L

H

G

G

F

Y

G

Q

E

E

G

G

I

I

A

A

L

L

A

D

N

V

T

N

G

G

L

Y

L

I

S

S

E
|
E

G

G

A
|
A

G
|
G

E
|
E

N
|
N

L

L

F

F

L

E

V

V

F

K

D

D

G

G

V

V

L

L

H

F

T

T

T

P

P

P

A

F

S

T

A

S

S

S

I

A

L
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L

T

P

G
|
G

I
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I

T
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T

R

R

H

D

T

A

L

I

I

I

T

K

L

L

A

A

R

K

E

E

D

L

G

G

I

I

E

E

V

V

I

R

E

E

R

Q

D

V

I

L

P

S

R

R

E

E

Y

S

L

L

Y

Y

L

L

C

A

D

D

E

E

L

V

L

F

M

M

C

S

G
|
G

T
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T

A
|
A

A

A

E

E

I

I

T

T

P

P

I

V

R

R

S

S

V

V

D

D

G

G

K

I

K

Q

I

V

G

G

S

E

G

G

K

R

A

C

G

G

R

P

V

V

T

T

R

K

R

R

M

I

Q

Q

E

Q

L

A

F

F

G

G

L

L

F

F

N

T

G

G

K

E

T

T

N

E

D

D

Q

K

W

W

G

G

W

W

L

L

E

D

P

Q

V

V

active site: F33 (= F37), G35 (= G39), K156 (= K158), A157 (= A159), E190 (= E192), L214 (= L216)
binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R60), Y92 (= Y96), R94 (= R98), Y126 (= Y128), K156 (= K158), Y161 (= Y163), E190 (= E192), A192 (= A194), G193 (= G195), E194 (= E196), N195 (= N197), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256), A255 (= A257)

1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
58% identity, 98% coverage: 7:305/305 of query aligns to 3:303/304 of 1iydA

query
sites
1iydA

E

D

W

Y

I

I

W

W

Q

F

N

N

G

G

Q

E

I

M

K

V

P

R

W

W

R

E

E

D

A

A

T

K

T

V

H

H

V

V

M

M

S

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

S

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

S

C

Y

Y

A

D

T

S

P

H

D

K

G

G

A

P

A

V

I

V

F

F

R

R

L

H

T

R

D

E

H

H

L

M

K

Q

R
|
R

L

L

Y

H

Q

D

S

S

A

A

K

K

I

I

Y

Y

D

R

M

F

V

P

L

V

P

S

Y

Q

S

S

Q

I

D

D

Q

E

I

L

A

M

A

E

A

A

C

C

R

R

D

D

V

V

I

I

K

R

Q

K

N

N

G

N

L

L

G

T

A

S

A

A

Y
|
Y

L

I

R
|
R

P

P

V

L

A

I

Y

F

R

V

G

G

L

D

G

V

G

G

F

M

G

G

L

V

S

N

-

P

A

A

E

G

T

Y

P

S

I

T

D

D

V

V

A

I

V

I

A

A

W

F

P

P

M
x
W

G

G

P

A

Y
|
Y

L

L

G

G

P

A

E

E

A

A

L

L

E

E

S

Q

G

G

I

I

D

D

A

A

C

M

V

V

S

S

W

W

Q

N

R

R

F

A

A

A

P

P

N

N

T

T

I

I

P

P

A

T

G

A

A

A

K
|
K

A
|
A

G

G

N

N

Y
|
Y

L

L

S

S

G

S

Q

L

L

L

I

V

A

G

R

S

E

E

A

A

R

R

L

H

G

G

F

Y

G

Q

E

E

G

G

I

I

A

A

L

L

A

D

N

V

T

N

G

G

L

Y

L

I

S

S

E
|
E

G

G

A

A

G
|
G

E
|
E

N
|
N

L

L

F

F

L

E

V

V

F

K

D

D

G

G

V

V

L

L

H

F

T

T

T

P

P

P

A

F

S

T

A

S

S

S

I

A

L
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L

T

P

G
|
G

I
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I

T
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T

R

R

H

D

T

A

L

I

I

I

T

K

L

L

A

A

R

K

E

E

D

L

G

G

I

I

E

E

V

V

I

R

E

E

R

Q

D

V

I

L

P

S

R

R

E

E

Y

S

L

L

Y

Y

L

L

C

A

D

D

E

E

L

V

L

F

M

M

C

S

G
|
G

T
|
T

A
|
A

A

A

E

E

I

I

T

T

P

P

I

V

R

R

S

S

V

V

D

D

G

G

K

I

K

Q

I

V

G

G

S

E

G

G

K

R

A

C

G

G

R

P

V

V

T

T

R

K

R

R

M

I

Q

Q

E

Q

L

A

F

F

G

G

L

L

F

F

N

T

G

G

K

E

T

T

N

E

D

D

Q

K

W

W

G

G

W

W

L

L

E

D

P

Q

V

V

active site: F33 (= F37), G35 (= G39), K156 (= K158), A157 (= A159), E190 (= E192), L214 (= L216)
binding glutaric acid: G35 (= G39), Y92 (= Y96), R94 (= R98), W123 (≠ M125), Y126 (= Y128), K156 (= K158), Y161 (= Y163), T254 (= T256), A255 (= A257)
binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K158), Y161 (= Y163), E190 (= E192), G193 (= G195), E194 (= E196), N195 (= N197), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256)

1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
58% identity, 98% coverage: 7:305/305 of query aligns to 3:303/304 of 1i1mA

query
sites
1i1mA

E

D

W

Y

I

I

W

W

Q

F

N

N

G

G

Q

E

I

M

K

V

P

R

W

W

R

E

E

D

A

A

T

K

T

V

H

H

V

V

M

M

S

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

S

S

V

V

F

F

E

E

G
|
G

I

I

R

R

S

C

Y

Y

A

D

T

S

P

H

D

K

G

G

A

P

A

V

I

V

F

F

R

R

L

H

T

R

D

E

H

H

L

M

K

Q

R
|
R

L

L

Y

H

Q

D

S

S

A

A

K

K

I

I

Y

Y

D

R

M

F

V

P

L

V

P

S

Y

Q

S

S

Q

I

D

D

Q

E

I

L

A

M

A

E

A

A

C

C

R

R

D

D

V

V

I

I

K

R

Q

K

N

N

G

N

L

L

G

T

A

S

A

A

Y
|
Y

L

I

R

R

P

P

V

L

A

I

Y

F

R

V

G

G

L

D

G

V

G

G

F

M

G

G

L

V

S

N

-

P

A

A

E

G

T

Y

P

S

I

T

D

D

V

V

A

I

V

I

A

A

W

F

P

P

M

W

G

G

P

A

Y

Y

L

L

G

G

P

A

E

E

A

A

L

L

E

E

S

Q

G

G

I

I

D

D

A

A

C

M

V

V

S

S

W

W

Q

N

R
|
R

F

A

A

A

P

P

N

N

T

T

I

I

P

P

A

T

G

A

A

A

K
|
K

A

A

G

G

N

N

Y
|
Y

L

L

S

S

G

S

Q

L

L

L

I

V

A

G

R

S

E

E

A

A

R

R

L

H

G

G

F

Y

G

Q

E

E

G

G

I

I

A

A

L

L

A

D

N

V

T

N

G

G

L

Y

L

I

S

S

E
|
E

G

G

A

A

G
|
G

E
|
E

N
|
N

L

L

F

F

L

E

V

V

F

K

D

D

G

G

V

V

L

L

H

F

T

T

T

P

P

P

A

F

S

T

A

S

S

S

I

A

L
|
L

T

P

G
|
G

I
|
I

T
|
T

R

R

H

D

T

A

L

I

I

I

T

K

L

L

A

A

R

K

E

E

D

L

G

G

I

I

E

E

V

V

I

R

E

E

R

Q

D

V

I

L

P

S

R

R

E

E

Y

S

L

L

Y

Y

L

L

C

A

D

D

E

E

L

V

L

F

M

M

C

S

G
|
G

T
|
T

A
|
A

A

A

E

E

I

I

T

T

P

P

I

V

R

R

S

S

V

V

D

D

G

G

K

I

K

Q

I

V

G

G

S

E

G

G

K

R

A

C

G

G

R

P

V

V

T

T

R

K

R

R

M

I

Q

Q

E

Q

L

A

F

F

G

G

L

L

F

F

N

T

G

G

K

E

T

T

N

E

D

D

Q

K

W

W

G

G

W

W

L

L

E

D

P

Q

V

V

active site: K156 (= K158)
binding 4-methyl valeric acid: G35 (= G39), Y92 (= Y96), K156 (= K158), Y161 (= Y163), G193 (= G195), T254 (= T256), A255 (= A257)
binding pyridoxal-5'-phosphate: R56 (= R60), R145 (= R147), K156 (= K158), Y161 (= Y163), E190 (= E192), G193 (= G195), E194 (= E196), N195 (= N197), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256)

1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
58% identity, 98% coverage: 7:305/305 of query aligns to 3:303/304 of 1i1lA

query
sites
1i1lA

E

D

W

Y

I

I

W

W

Q

F

N

N

G

G

Q

E

I

M

K

V

P

R

W

W

R

E

E

D

A

A

T

K

T

V

H

H

V

V

M

M

S

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

S

S

V

V

F

F

E

E

G
|
G

I

I

R

R

S

C

Y

Y

A

D

T

S

P

H

D

K

G

G

A

P

A

V

I

V

F

F

R

R

L

H

T

R

D

E

H

H

L

M

K

Q

R
|
R

L

L

Y

H

Q

D

S

S

A

A

K

K

I

I

Y

Y

D

R

M

F

V

P

L

V

P

S

Y

Q

S

S

Q

I

D

D

Q

E

I

L

A

M

A

E

A

A

C

C

R

R

D

D

V

V

I

I

K

R

Q

K

N

N

G

N

L

L

G

T

A

S

A

A

Y
|
Y

L

I

R

R

P

P

V

L

A

I

Y

F

R

V

G

G

L

D

G

V

G

G

F

M

G

G

L

V

S

N

-

P

A

A

E

G

T

Y

P

S

I

T

D

D

V

V

A

I

V

I

A

A

W

F

P

P

M
x
W

G

G

P

A

Y
|
Y

L

L

G

G

P

A

E

E

A

A

L

L

E

E

S

Q

G

G

I

I

D

D

A

A

C

M

V

V

S

S

W

W

Q

N

R

R

F

A

A

A

P

P

N

N

T

T

I

I

P

P

A

T

G

A

A

A

K
|
K

A

A

G

G

N

N

Y
|
Y

L

L

S

S

G

S

Q

L

L

L

I

V

A

G

R

S

E

E

A

A

R

R

L

H

G

G

F

Y

G

Q

E

E

G

G

I

I

A

A

L

L

A

D

N

V

T

N

G

G

L

Y

L

I

S

S

E
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E

G

G

A

A

G
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G

E
|
E

N
|
N

L

L

F

F

L

E

V

V

F

K

D

D

G

G

V

V

L

L

H

F

T

T

T

P

P

P

A

F

S

T

A

S

S

S

I

A

L
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L

T

P

G
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G

I
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I

T
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T

R

R

H

D

T

A

L

I

I

I

T

K

L

L

A

A

R

K

E

E

D

L

G

G

I

I

E

E

V

V

I

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E

E

R

Q

D

V

I

L

P

S

R

R

E

E

Y

S

L

L

Y

Y

L

L

C

A

D

D

E

E

L

V

L

F

M

M

C

S

G
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G

T
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T

A
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A

A

A

E

E

I

I

T

T

P

P

I

V

R

R

S

S

V

V

D

D

G

G

K

I

K

Q

I

V

G

G

S

E

G

G

K

R

A

C

G

G

R

P

V

V

T

T

R

K

R

R

M

I

Q

Q

E

Q

L

A

F

F

G

G

L

L

F

F

N

T

G

G

K

E

T

T

N

E

D

D

Q

K

W

W

G

G

W

W

L

L

E

D

P

Q

V

V

active site: K156 (= K158)
binding 2-methylleucine: G35 (= G39), Y92 (= Y96), W123 (≠ M125), Y126 (= Y128), K156 (= K158), G193 (= G195), T254 (= T256), A255 (= A257)
binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K158), Y161 (= Y163), E190 (= E192), G193 (= G195), E194 (= E196), N195 (= N197), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256)

4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
48% identity, 97% coverage: 9:305/305 of query aligns to 9:306/306 of 4whxA

query
sites
4whxA

I

I

W

W

Q

M

N

D

G

G

Q

K

I

L

K

I

P

E

W

W

R

R

E

D

A

A

T

K

T

I

H

H

V

V

M

L

S

T

H

H

A

T

L

L

H

H

Y

Y

G

G

S

M

S

G

V

V

F
|
F

E

E

G

G

I

V

R

R

S

A

Y

Y

A

K

T

T

P

A

D

D

G

G

-

G

A

T

A

A

I

I

F

F

R

R

L

L

T

K

D

E

H

H

L

T

K

K

R

R

L

L

Y

L

Q

N

S

S

A

A

K

K

I

I

Y

F

D

Q

M

M

V

D

L

V

P

P

Y

F

S

D

Q

Q

D

E

Q

T

I

L

A

E

A

A

C

Q

R

R

D

D

V

V

I

V

K

R

Q

E

N

N

G

K

L

L

G

E

A

S

A

C

Y
|
Y

L

L

R

R

P

P

V

I

A

I

Y

W

R

I

G

G

L

S

G

E

G

K

F

L

G

G

L

V

S

S

A

A

E

K

-

G

T

N

P

T

I

I

D

H

V

V

A

A

V

I

A

A

W

W

P

P

M

W

G

G

P

A

Y

Y

L

L

G

G

P

E

E

E

A

G

L

L

E

A

S

K

G

G

I

I

D

R

A

V

C

K

V

T

S

S

W

F

Q

T

R

R

F

H

A

H

P

V

N

N

T

V

I

S

P

M

A

V

G

R

A

A

K
|
K

A

A

G

S

G

G

N

W

Y

Y

L

V

S

N

G

S

Q

I

L

L

I

A

A

N

R

Q

E

E

A

A

R

T

R

A

L

D

G

G

F

Y

G

D

E

E

G

A

I

L

A

L

L

L

A

D

N

V

T

D

G

G

L

Y

L

V

S

S

E
|
E

G

G

A

S

G
|
G

E

E

N

N

L

F

F

F

L

L

V

V

F

N

D

R

G

G

V

K

L

L

H

Y

T

T

T

-

P

P

A

D

S

L

A

A

S

S

I

C

L
|
L

T

D

G

G

I

I

T

T

R

R

H

D

T

T

L

V

I

I

T

T

L

L

A

A

R

K

E

E

D

A

G

G

I

I

E

E

V

V

I

I

E

E

R

K

D

R

I

I

P

T

R

R

E

D

Y

E

L

V

Y

Y

L

T

C

A

D

D

E

E

L

A

L

F

M

F

C

T

G

G

T
|
T

A
|
A

A

A

E

E

I

V

T

T

P

P

I

I

R

R

S

E

V

L

D

D

G

N

K

R

K

T

I

I

G

G

S

G

G

G

K

A

A

R

G

G

R

P

V

I

T

T

R

E

R

K

M

L

Q

Q

E

S

L

A

F

F

G

D

L

V

F

V

N

N

G

G

K

K

T

S

N

A

D

K

Q

H

W

A

G

D

W

W

L

L

E

T

P

K

V

I

active site: F37 (= F37), K160 (= K158), E194 (= E192), L217 (= L216)
binding alanine: Y97 (= Y96), K160 (= K158), G197 (= G195), T257 (= T256), A258 (= A257)

2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
44% identity, 96% coverage: 9:302/305 of query aligns to 7:301/305 of 2ej0B

query
sites
2ej0B

I

I

W

W

Q

M

N

N

G

G

Q

A

I

F

K

V

P

P

W

Q

R

E

E

E

A

A

T

K

T

T

H

S

V

V

M

L

S

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

S

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

S

A

Y

Y

A

E

T

T

P

A

D

K

G

G

A

P

A

A

I

I

F

F

R

R

L

L

T

K

D

E

H

H

L

V

K

K

R
|
R

L

F

Y

Y

Q

N

S

S

A

A

K

K

I

V

Y

L

D

R

M

M

V

E

L

I

P

P

Y

F

S

A

Q

P

D

E

Q

E

I

L

A

E

A

A

C

I

R

K

D

E

V

V

I

V

K

R

Q

R

N

N

G

G

L

Y

G

R

A

S

A

C

Y

Y

L

I

R

R

P

P

V

L

A

A

Y

W

R

M

G

G

L

A

G

K

G

A

F

L

G

G

L

V

S

N

-

P

-

L

A

P

E

N

T

N

P

P

I

A

D

E

V

V

A

M

V

V

A

A

W

W

P

E

M

W

G

G

P

A

Y

Y

L

L

G

G

P

E

E

E

A

A

L

V

E

R

S

K

G

G

I

A

D

R

A

L

C

I

V

T

S

S

W

W

Q

A

R

R

F

F

A

P

P

A

N

N

T

V

I

M

P

P

A

G

G

K

A

A

K
|
K

A

V

G

G

N

N

Y
|
Y

L

V

S

N

G

S

Q

A

L

L

I

A

A

K

R

M

E

E

A

A

R

V

R

A

L

A

G

G

F

A

G

D

E

E

G

A

I

L

A

L

L

L

A

D

N

E

T

E

G

G

L

Y

L

V

S

A

E
|
E

G

G

A

S

G
|
G

E
|
E

N
|
N

L

L

F

F

L

F

V

V

F

R

D

D

G

G

V

V

L

I

H

Y

T

A

T

L

P

E

A

H

S

S

A

V

S

N

I

-

L
|
L

T

E

G
|
G

I
|
I

T
|
T

R

R

H

D

T

S

L

V

I

I

T

R

L

I

A

A

R

K

E

D

D

L

G

G

I

Y

E

E

V

V

I

Q

E

V

R

V

D

R

I

A

P

T

R

R

E

D

Y

Q

L

L

Y

Y

L

M

C

A

D

D

E

E

L

V

L

F

M

M

C

T

G
|
G

T
|
T

A

A

E

E

I

V

T

T

P

P

I

V

R

S

S

M

V

I

D

D

G

W

K

R

K

P

I

I

G

G

S

K

G

G

K

T

A

A

G

G

R

P

V

V

T

A

R

L

R

R

M

L

Q

R

E

E

L

V

F

Y

F

L

G

E

L

A

F

V

N

T

G

G

K

R

T

R

N

P

D

E

Q

Y

W

E

G

G

W

W

L

L

active site: F35 (= F37), G37 (= G39), K158 (= K158), E192 (= E192), L215 (= L216)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R60), K158 (= K158), Y163 (= Y163), E192 (= E192), G195 (= G195), E196 (= E196), N197 (= N197), L215 (= L216), G217 (= G218), I218 (= I219), T219 (= T220), G254 (= G255), T255 (= T256)

2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
43% identity, 96% coverage: 9:302/305 of query aligns to 7:293/297 of 2ej3A

query
sites
2ej3A

I

I

W

W

Q

M

N

N

G

G

Q

A

I

F

K

V

P

P

W

Q

R

E

E

E

A

A

T

K

T

T

H

S

V

V

M

L

S

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

S

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

S

A

Y

Y

A

E

T

T

P

A

D

K

G

G

A

P

A

A

I

I

F

F

R

R

L

L

T

K

D

E

H

H

L

V

K

K

R
|
R

L

F

Y

Y

Q

N

S

S

A

A

K

K

I

V

Y

L

D

R

M

M

V

E

L

I

P

P

Y

F

S

A

Q

P

D

E

Q

E

I

L

A

E

A

A

C

I

R

K

D

E

V

V

I

V

K

R

Q

R

N

N

G

G

L

Y

G

R

A

S

A

C

Y
|
Y

L

I

R
|
R

P

P

V

L

A

A

Y

W

R

M

G

G

L

A

G

K

G

A

F

L

G

G

L

V

S

N

-

P

-

L

A

P

E

N

T

N

P

P

I

A

D

E

V

V

A

M

V

V

A

A

W

W

P

-

M

-

G

-

P

-

Y

-

L

-

G

-

P

-

E

E

A

W

L

V

E

R

S

K

G

G

I

A

D

R

A

L

C

I

V

T

S

S

W

W

Q

A

R
|
R

F

F

A

P

P

A

N

N

T

V

I

M

P

P

A

G

G

K

A

A

K
|
K

A

V

G

G

N

N

Y
|
Y

L

V

S

N

G

S

Q

A

L

L

I

A

A

K

R

M

E

E

A

A

R

V

R

A

L

A

G

G

F

A

G

D

E

E

G

A

I

L

A

L

L

L

A

D

N

E

T

E

G

G

L

Y

L

V

S

A

E
|
E

G

G

A

S

G
|
G

E
|
E

N

N

L

L

F

F

L

F

V

V

F

R

D

D

G

G

V

V

L

I

H

Y

T

A

T

L

P

E

A

H

S

S

A

V

S

N

I

-

L
|
L

T

E

G
|
G

I
|
I

T
|
T

R

R

H

D

T

S

L

V

I

I

T

R

L

I

A

A

R

K

E

D

D

L

G

G

I

Y

E

E

V

V

I

Q

E

V

R

V

D

R

I

A

P

T

R

R

E

D

Y

Q

L

L

Y

Y

L

M

C

A

D

D

E

E

L

V

L

F

M

M

C

T

G
|
G

T
|
T

A
|
A

E

E

I

V

T

T

P

P

I

V

R

S

S

M

V

I

D

D

G

W

K

R

K

P

I

I

G

G

S

K

G

G

K

T

A

A

G

G

R

P

V

V

T

A

R

L

R

R

M

L

Q

R

E

E

L

V

F

Y

F

L

G

E

L

A

F

V

N

T

G

G

K

R

T

R

N

P

D

E

Q

Y

W

E

G

G

W

W

L

L

active site: F35 (= F37), G37 (= G39), K150 (= K158), E184 (= E192), L207 (= L216)
binding [1-(aminomethyl)cyclohexyl]acetic acid: Y94 (= Y96), R96 (= R98), Y155 (= Y163), G187 (= G195), E188 (= E196), G246 (= G255), T247 (= T256), A248 (= A257), A249 (= A258)
binding pyridoxal-5'-phosphate: R58 (= R60), R139 (= R147), K150 (= K158), Y155 (= Y163), E184 (= E192), G187 (= G195), E188 (= E196), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), G246 (= G255), T247 (= T256)

2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
43% identity, 96% coverage: 9:302/305 of query aligns to 7:293/297 of 2eiyA

query
sites
2eiyA

I

I

W

W

Q

M

N

N

G

G

Q

A

I

F

K

V

P

P

W

Q

R

E

E

E

A

A

T

K

T

T

H

S

V

V

M

L

S

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

S

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

S

A

Y

Y

A

E

T

T

P

A

D

K

G

G

A

P

A

A

I

I

F

F

R

R

L

L

T

K

D

E

H

H

L

V

K

K

R
|
R

L

F

Y

Y

Q

N

S

S

A

A

K

K

I

V

Y

L

D

R

M

M

V

E

L

I

P

P

Y

F

S

A

Q

P

D

E

Q

E

I

L

A

E

A

A

C

I

R

K

D

E

V

V

I

V

K

R

Q

R

N

N

G

G

L

Y

G

R

A

S

A

C

Y
|
Y

L

I

R

R

P

P

V

L

A

A

Y

W

R

M

G

G

L

A

G

K

G

A

F

L

G

G

L

V

S

N

-

P

-

L

A

P

E

N

T

N

P

P

I

A

D

E

V

V

A

M

V

V

A

A

W

W

P

-

M

-

G

-

P

-

Y

-

L

-

G

-

P

-

E

E

A

W

L

V

E

R

S

K

G

G

I

A

D

R

A

L

C

I

V

T

S

S

W

W

Q

A

R
|
R

F

F

A

P

P

A

N

N

T

V

I

M

P

P

A

G

G

K

A

A

K
|
K

A

V

G

G

N

N

Y
|
Y

L

V

S

N

G

S

Q

A

L

L

I

A

A

K

R

M

E

E

A

A

R

V

R

A

L

A

G

G

F

A

G

D

E

E

G

A

I

L

A

L

L

L

A

D

N

E

T

E

G

G

L

Y

L

V

S

A

E
|
E

G

G

A

S

G
|
G

E
|
E

N
|
N

L

L

F

F

L

F

V

V

F

R

D

D

G

G

V

V

L

I

H

Y

T

A

T

L

P

E

A

H

S

S

A

V

S

N

I

-

L
|
L

T

E

G
|
G

I
|
I

T
|
T

R

R

H

D

T

S

L

V

I

I

T

R

L

I

A

A

R

K

E

D

D

L

G

G

I

Y

E

E

V

V

I

Q

E

V

R

V

D

R

I

A

P

T

R

R

E

D

Y

Q

L

L

Y

Y

L

M

C

A

D

D

E

E

L

V

L

F

M

M

C

T

G
|
G

T
|
T

A
|
A

A

A

E

E

I

V

T

T

P

P

I

V

R

S

S

M

V

I

D

D

G

W

K

R

K

P

I

I

G

G

S

K

G

G

K

T

A

A

G

G

R

P

V

V

T

A

R

L

R

R

M

L

Q

R

E

E

L

V

F

Y

F

L

G

E

L

A

F

V

N

T

G

G

K

R

T

R

N

P

D

E

Q

Y

W

E

G

G

W

W

L

L

active site: F35 (= F37), G37 (= G39), K150 (= K158), E184 (= E192), L207 (= L216)
binding 4-methyl valeric acid: F35 (= F37), Y94 (= Y96), Y155 (= Y163), T247 (= T256), A248 (= A257)
binding pyridoxal-5'-phosphate: R58 (= R60), R139 (= R147), K150 (= K158), Y155 (= Y163), E184 (= E192), G187 (= G195), E188 (= E196), N189 (= N197), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), G246 (= G255), T247 (= T256)

1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
43% identity, 96% coverage: 9:302/305 of query aligns to 7:293/297 of 1wrvA

query
sites
1wrvA

I

I

W

W

Q

M

N

N

G

G

Q

A

I

F

K

V

P

P

W

Q

R

E

E

E

A

A

T

K

T

T

H

S

V

V

M

L

S

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

S

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

S

A

Y

Y

A

E

T

T

P

A

D

K

G

G

A

P

A

A

I

I

F

F

R

R

L

L

T

K

D

E

H

H

L

V

K

K

R
|
R

L

F

Y

Y

Q

N

S

S

A

A

K

K

I

V

Y

L

D

R

M

M

V

E

L

I

P

P

Y

F

S

A

Q

P

D

E

Q

E

I

L

A

E

A

A

C

I

R

K

D

E

V

V

I

V

K

R

Q

R

N

N

G

G

L

Y

G

R

A

S

A

C

Y

Y

L

I

R

R

P

P

V

L

A

A

Y

W

R

M

G

G

L

A

G

K

G

A

F

L

G

G

L

V

S

N

-

P

-

L

A

P

E

N

T

N

P

P

I

A

D

E

V

V

A

M

V

V

A

A

W

W

P

-

M

-

G

-

P

-

Y

-

L

-

G

-

P

-

E

E

A

W

L

V

E

R

S

K

G

G

I

A

D

R

A

L

C

I

V

T

S

S

W

W

Q

A

R
|
R

F

F

A

P

P

A

N

N

T

V

I

M

P

P

A

G

G

K

A

A

K
|
K

A

V

G

G

N

N

Y
|
Y

L

V

S

N

G

S

Q

A

L

L

I

A

A

K

R

M

E

E

A

A

R

V

R

A

L

A

G

G

F

A

G

D

E

E

G

A

I

L

A

L

L

L

A

D

N

E

T

E

G

G

L

Y

L

V

S

A

E
|
E

G

G

A

S

G
|
G

E
|
E

N
|
N

L

L

F

F

L

F

V

V

F

R

D

D

G

G

V

V

L

I

H

Y

T

A

T

L

P

E

A

H

S

S

A

V

S

N

I

-

L
|
L

T

E

G
|
G

I
|
I

T
|
T

R

R

H

D

T

S

L

V

I

I

T

R

L

I

A

A

R

K

E

D

D

L

G

G

I

Y

E

E

V

V

I

Q

E

V

R

V

D

R

I

A

P

T

R

R

E

D

Y

Q

L

L

Y

Y

L

M

C

A

D

D

E

E

L

V

L

F

M

M

C

T

G
|
G

T
|
T

A

A

E

E

I

V

T

T

P

P

I

V

R

S

S

M

V

I

D

D

G

W

K

R

K

P

I

I

G

G

S

K

G

G

K

T

A

A

G

G

R

P

V

V

T

A

R

L

R

R

M

L

Q

R

E

E

L

V

F

Y

F

L

G

E

L

A

F

V

N

T

G

G

K

R

T

R

N

P

D

E

Q

Y

W

E

G

G

W

W

L

L

active site: F35 (= F37), G37 (= G39), K150 (= K158), E184 (= E192), L207 (= L216)
binding pyridoxal-5'-phosphate: R58 (= R60), R139 (= R147), K150 (= K158), Y155 (= Y163), E184 (= E192), G187 (= G195), E188 (= E196), N189 (= N197), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), G246 (= G255), T247 (= T256)

2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
43% identity, 96% coverage: 9:302/305 of query aligns to 7:290/294 of 2ej2A

query
sites
2ej2A

I

I

W

W

Q

M

N

N

G

G

Q

A

I

F

K

V

P

P

W

Q

R

E

E

E

A

A

T

K

T

T

H

S

V

V

M

L

S

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

S

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

S

A

Y

Y

A

E

T

T

P

A

D

K

G

G

A

P

A

A

I

I

F

F

R

R

L

L

T

K

D

E

H

H

L

V

K

K

R
|
R

L

F

Y

Y

Q

N

S

S

A

A

K

K

I

V

Y

L

D

R

M

M

V

E

L

I

P

P

Y

F

S

A

Q

P

D

E

Q

E

I

L

A

E

A

A

C

I

R

K

D

E

V

V

I

V

K

R

Q

R

N

N

G

G

L

Y

G

R

A

S

A

C

Y
|
Y

L

I

R

R

P

P

V

L

A

A

Y

W

R

M

G

G

L

A

G

K

G

A

F

L

G

G

L

V

S

N

-

P

-

L

A

P

E

N

T

N

P

P

I

A

D

E

V

V

A

M

V

V

A

A

W

W

P

E

M

W

G

G

P

A

Y

R

L

L

G

-

P

-

E

-

A

-

L

-

E

-

S

-

G

-

I

-

D

-

A

-

C

I

V

T

S

S

W

W

Q

A

R
|
R

F

F

A

P

P

A

N

N

T

V

I

M

P

P

A

G

G

K

A

A

K
|
K

A

V

G

G

N

N

Y
|
Y

L

V

S

N

G

S

Q

A

L

L

I

A

A

K

R

M

E

E

A

A

R

V

R

A

L

A

G

G

F

A

G

D

E

E

G

A

I

L

A

L

L

L

A

D

N

E

T

E

G

G

L

Y

L

V

S

A

E
|
E

G

G

A
x
S

G
|
G

E
|
E

N
|
N

L

L

F

F

L

F

V

V

F

R

D

D

G

G

V

V

L

I

H

Y

T

A

T

L

P

E

A

H

S

S

A

V

S

N

I

-

L
|
L

T

E

G
|
G

I
|
I

T
|
T

R

R

H

D

T

S

L

V

I

I

T

R

L

I

A

A

R

K

E

D

D

L

G

G

I

Y

E

E

V

V

I

Q

E

V

R

V

D

R

I

A

P

T

R

R

E

D

Y

Q

L

L

Y

Y

L

M

C

A

D

D

E

E

L

V

L

F

M

M

C

T

G
|
G

T
|
T

A
|
A

A

A

E

E

I

V

T

T

P

P

I

V

R

S

S

M

V

I

D

D

G

W

K

R

K

P

I

I

G

G

S

K

G

G

K

T

A

A

G

G

R

P

V

V

T

A

R

L

R

R

M

L

Q

R

E

E

L

V

F

Y

F

L

G

E

L

A

F

V

N

T

G

G

K

R

T

R

N

P

D

E

Q

Y

W

E

G

G

W

W

L

L

active site: F35 (= F37), G37 (= G39), K147 (= K158), E181 (= E192), L204 (= L216)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: G37 (= G39), R58 (= R60), Y94 (= Y96), R136 (= R147), K147 (= K158), Y152 (= Y163), E181 (= E192), S183 (≠ A194), G184 (= G195), E185 (= E196), N186 (= N197), L204 (= L216), G206 (= G218), I207 (= I219), T208 (= T220), G243 (= G255), T244 (= T256), A245 (= A257)

6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
40% identity, 97% coverage: 9:305/305 of query aligns to 9:300/301 of 6thqB

query
sites
6thqB

I

V

W

W

Q

L

N

D

G

G

Q

R

I

L

K

V

P

D

W

E

R

E

E

E

A

A

T

K

T

V

H

T

V

V

M

L

S

S

H

P

A

S

L

L

H

N

Y
|
Y

G

G

S

F

S

G

V

V

F
|
F

E

E

G

G

I

I

R

R

S

A

Y

Y

A

W

T

N

P

G

D

E

G

N

A

L

A

Y

I

V

F

F

R

R

L

L

T

R

D

D

H

H

L

M

K

E

R
|
R

L

L

Y

L

Q

R

S

S

A

A

K

K

I

I

Y

I

D

G

M

L

V

D

L

V

P

P

Y

Y

S

T

Q

A

D

E

Q

E

I

L

A

S

A

K

A

A

C

V

R

V

D

E

V

T

I

V

K

R

Q

A

N

N

G

G

L

F

G

K

A

E

-

D

A

L

Y
|
Y

L

I

R

R

P

P

V

V

A

A

Y

Y

R

I

G

S

L

K

G

P

G

Q

F
x
I

G

S

L

L

S

D

A

V

E

R

-

G

T

L

P

Q

I

A

D

S

V

V

A

A

V

I

A

A

W

I

P

P

M

F

G

G

P

K

Y

Y

L

L

G

K

P

V

E

E

A

-

L

-

E

-

S

-

G

G

I

V

D

R

A

A

C

A

V

V

S

V

S

S

W

W

Q

R

R
|
R

F

V

A

H

P

T

N

S

T

M

I

M

P

P

A

V

G

M

A

A

K
|
K

A

A

G

T

G

G

N

I

Y
|
Y

L

L

S

N

G

S

Q

I

L

M

I

A

A

A

R

V

E

E

A

A

R

R

R

A

L

R

G

G

F

Y

G

D

E

E

G

A

I

I

A

M

L

L

A

N

N

A

T

E

G

G

L

K

L

V

S

V

E
|
E

G

G

A
x
S

G
|
G

E
|
E

N
|
N

L

I

F

F

L

I

V

V

F

R

D

R

G

G

V

V

L

L

H

M

T

T

T

P

P

P

A

L

S

E

A

D

S

G

I

I

L
|
L

T

E

G
|
G

I
|
I

T
|
T

R

R

H

E

T

T

L

V

I

I

T

S

L

I

A

A

R

G

E

D

D

L

G

G

I

I

E

P

V

L

I

L

E

E

R

K

D

S

I

I

P

T

R

R

E

E

Y

E

L

L

Y

Y

L

A

C

A

D

D

E

E

L

A

L

F

M

F

C

V

G
|
G

T
|
T

A
|
A

A

A

E

E

I

I

T

T

P

P

I

I

R

I

S

E

V

I

D

D

G

G

K

R

K

V

I

L

G

-

S

-

G

-

K

Q

A

R

G

G

R

P

V

I

T

T

R

Q

R

K

M

I

Q

A

E

E

L

T

F

Y

F

R

G

R

L

I

F

V

N

L

G

G

K

K

T

E

N

E

D

K

Q

Y

W

L

G

P

W

W

L

L

E

T

P

P

V

V

active site: F37 (= F37), K156 (= K158), E190 (= E192), L214 (= L216)
binding pyridoxal-5'-phosphate: R60 (= R60), R145 (= R147), K156 (= K158), Y161 (= Y163), E190 (= E192), G193 (= G195), E194 (= E196), N195 (= N197), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256)
binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: Y32 (= Y32), R60 (= R60), Y97 (= Y96), I109 (≠ F108), K156 (= K158), Y161 (= Y163), E190 (= E192), S192 (≠ A194), G193 (= G195), E194 (= E196), N195 (= N197), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256), A255 (= A257)

6h65C Crystal structure of the branched-chain-amino-acid aminotransferase from haliangium ochraceum
36% identity, 98% coverage: 6:305/305 of query aligns to 4:306/308 of 6h65C

query
sites
6h65C

P

P

E

R

W

Y

I

G

W

W

Q

M

N

N

G

G

Q

Q

I

C

K

I

P

P

W

W

R

D

E

Q

A

C

T

S

T

L

H

H

V

V

M

S

S

T

H

Q

A

A

L

A

H

F

Y

F

G

G

S

A

S

S

V

L

F
|
F

E

E

G

G

I

V

R

R

S

A

Y

Y

-

W

-

N

A

A

T

E

P

R

D

E

G

Q

A

L

A

Y

I

V

F

F

R

R

L

L

T

D

D

E

H
|
H

L

L

K

R

R
|
R

L

L

Y

E

Q

Q

S

S

A

A

K

K

I

M

Y

L

D

R

M

M

V

K

L

L

P

S

Y

M

S

P

Q

I

D

A

Q

D

I

I

A

R

A

Q

A

G

C

V

R

L

D

E

V

L

I

L

K

R

Q

A

N

N

G

E

L

F

G

R

A

S

-

D

A

V

Y

H

L

L

R

Y

P

V

V

A

A

S

Y

Y

R

-

G

-

L

-

G

-

F

F

G

G

L

I

S

N

A

H

E

D

T

P

-

D

-

P

-

L

-

F

P

P

I

T

D

D

-

D

-

T

-

G

-

V

-

Y

-

V

-

T

-

G

V

T

A

A

V

V

A

S

A

R

W

L

P

P

M

L

G

-

P

-

Y

-

L

-

G

-

P

-

E

-

A

-

L

V

E

H

S

T

G

G

I

I

D

S

A

A

C

C

V

M

S

S

W

W

Q

R

R

R

F

I

A

S

P

D

N

D

T

S

I

V

P

P

A

P

G

R

A

I

K
|
K

A

I

G

G

G

A

N

N

Y
|
Y

L

Q

S

N

G

S

Q

R

L

L

I

A

A

Q

R

T

E

E

A

A

R

R

R

V

L

N

G

G

F

Y

G

H

E

T

G

S

I

V

A

L

L

L

A

N

N

S

T

R

G

G

L

K

L

V

S

S

E
|
E

G

T

A
x
P

G
|
G

E
x
A

N
x
C

L

L

F

L

L

M

V

V

F

R

D

D

G

G

V

R

L

V

H

I

T

S

T

P

P

P

A

V

S

T

A

A

S

D

I

I

L
|
L

T

E

G
x
S

I
x
V

T
|
T

R

R

H

K

T

T

L

L

I

M

T

S

L

L

A

S

R

E

-

A

E

E

D

L

G

D

I

S

E

P

V

V

I

I

E

E

R

R

D

D

I

M

P

D

R

R

E

T

Y

E

L

L

Y

Y

L

I

C

A

D

E

E

E

L

V

L

F

M

L

C

C

G
|
G

T
|
T

A

I

A

A

E

E

I

I

T

L

P

P

I

V

R

T

S

T

V

I

D

D

G

R

K

I

K

Q

I

V

G

G

S

D

G

G

K

E

A

V

G

G

R

P

V

V

T

T

R

R

M

L

Q

Q

E

E

L

L

F

Y

F

F

G

G

L

V

F

T

N

S

G

G

K

Q

T

L

N

E

D

A

Q

Y

W

K

G

S

W

W

L

L

E

L

P

P

V

V

active site: F35 (= F37), K158 (= K158), E192 (= E192), L216 (= L216)
binding pyridoxal-5'-phosphate: H57 (= H57), R60 (= R60), K158 (= K158), Y163 (= Y163), E192 (= E192), P194 (≠ A194), G195 (= G195), A196 (≠ E196), C197 (≠ N197), L216 (= L216), S218 (≠ G218), V219 (≠ I219), T220 (= T220), G256 (= G255), T257 (= T256)

5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
37% identity, 93% coverage: 8:290/305 of query aligns to 3:280/290 of 5mr0D

query
sites
5mr0D

W

Y

I

V

W

Y

Q

M

N

D

G

G

Q

E

I

F

K

V

P

P

W

E

R

N

E

E

A

A

T

K

T

V

H

S

V

I

M

F

S

D

H

H

A

G

L

F

H

L

Y

Y

G

G

S

D

S

G

V

V

F
|
F

E

E

G
|
G

I

I

R

R

S

A

Y

Y

A

-

T

-

P

-

D

-

G

N

A

G

A

R

I

V

F

F

R

R

L

L

T

K

D

E

H

H

L

I

K

D

R
|
R

L

L

Y

Y

Q

D

S

S

A

A

K

K

I

A

Y

I

D

D

M

L

V

E

L

I

P

P

Y

I

S

T

Q

K

D

E

Q

E

I

F

A

M

A

E

A

I

C

I

R

L

D

E

V

T

I

L

K

R

Q

K

N

N

G

N

L

L

G

R

A

D

A

A

Y

Y

L

I

R
|
R

P

P

V

I

A

V

Y

T

R

R

G

G

L

I

G

G

G

D

F

L

G
|
G

L
|
L

S

D

-

P

-

R

-

K

A

C

E

Q

T

N

P

P

I

-

D

S

V

I

A

I

V

V

A

I

A

T

W

K

P

P

M

W

G

G

P

K

Y

L

L

Y

G

G

P

-

E

D

A

L

L

Y

E

E

S

K

G

G

I

L

D

T

A

A

C

I

V

T

S

V

S

A

W

V

Q

R

R

R

F

N

A

S

P

F

N

D

T

A

I

L

P

P

A

P

G

N

A

I

K
|
K

A

S

G

L

G

-

N

N

Y
|
Y

L

L

S

N

G

N

Q

I

L

L

I

A

A

K

R

I

E

E

A

A

R

N

R

A

L

K

G

G

F

G

G

D

E

E

G

A

I

I

A

F

L

L

A

D

N

R

T

N

G

G

L

Y

L

V

S

S

E
|
E

G

G

A

S

G
|
G

E
x
D

N
|
N

L

I

F

F

L

V

V

V

F

K

D

N

G

G

V

A

L

I

H

-

T

T

P

P

A

P

S

T

A

I

S

N

I

N

L
|
L

T

R

G
|
G

I
|
I

T
|
T

R

R

H

E

T

A

L

V

I

I

T

E

L

I

A

I

R

N

E

R

D

L

G

G

I

I

E

P

V

F

I

K

E

E

R

T

D

N

I

I

P

G

R

L

E

Y

Y

D

L

L

Y

Y

L

T

C

A

D

D

E

E

L

V

L

F

M

V

C

T

G
|
G

T
|
T

A

A

E

E

I

I

T

A

P

P

I

I

R

V

S

V

V

I

D

D

G

G

K

R

K

K

I

I

G

G

S

D

G

G

K

K

A

P

G

G

R

E

V

I

T

T

R

R

R

K

M

L

Q

M

E

E

L

E

F

F

F

S

G

K

L

L

active site: F32 (= F37), G34 (= G39), K150 (= K158), E183 (= E192), L206 (= L216)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: F32 (= F37), R51 (= R60), R89 (= R98), G100 (= G109), L101 (= L110), K150 (= K158), Y154 (= Y163), E183 (= E192), G186 (= G195), D187 (≠ E196), N188 (= N197), L206 (= L216), G208 (= G218), I209 (= I219), T210 (= T220), G245 (= G255), T246 (= T256)

5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
37% identity, 91% coverage: 9:287/305 of query aligns to 5:278/290 of 5e25A

query
sites
5e25A

I

V

W

Y

Q

M

N

N

G

G

Q

E

I

F

K

V

P

P

W

E

R

S

E

Q

A

A

T

K

T

V

H

S

V

V

M

F

S

D

H

H

A

G

L

F

H

L

Y

Y

G

G

S

D

S

G

V

V

F
|
F

E

E

G
|
G

I

I

R

R

S

A

Y

Y

A

-

T

-

P

-

D

-

G

N

A

G

A

K

I

V

F

F

R

K

L

L

T

Y

D

E

H

H

L

I

K

D

R
|
R

L

L

Y

Y

Q

D

S

C

A

A

K

R

I

V

Y

I

D

D

M

L

V

K

L

I

P

P

Y

L

S

S

Q

K

D

E

Q

E

I

F

A

A

A

E

A

A

C

I

R

L

D

E

V

T

I

L

K

R

Q

R

N

N

G

N

L

L

G

R

A

D

A

A

Y
|
Y

L

I

R
|
R

P

P

V

I

A

V

Y

T

R

R

G

G

L

A

G

G

G

D

F

L

G

G

L

L

S

D

A

P

E

R

-

K

-

C

-

P

T

S

P

P

I

-

D

N

V

V

A

I

V

I

A

I

A

T

W

K

P

P

M

W

G

G

P

K

Y

L

L

Y

G

G

P

-

E

D

A

L

L

Y

E

E

S

K

G

G

I

L

D

K

A

A

C

I

V

T

S

V

S

A

W

I

Q

R

R

R

F

N

A

A

P

I

N

D

T

S

I

L

P

P

A

P

G

N

A

I

K
|
K

A

S

G

L

G

-

N

N

Y
|
Y

L

L

S

N

G

N

Q

I

L

L

I

A

A

K

R

I

E

E

A

A

R

N

R

A

L

K

G

G

F

G

G

D

E

E

G

A

I

I

A

F

L

L

A

D

N

H

T

N

G

G

L

Y

L

I

S

S

E
|
E

G

G

A

S

G
|
G

E
x
D

N
|
N

L

I

F

F

L

I

V

V

F

K

D

N

G

G

V

T

L

I

H

-

T

T

P

P

A

P

S

T

A

L

S

N

I

N

L
|
L

T

K

G
|
G

I
|
I

T
|
T

R

R

H

Q

T

V

L

V

I

I

T

E

L

L

A

I

R

N

E

E

D

L

G

E

I

I

E

P

V

F

I

R

E

E

R

A

D

N

I

I

P

G

R

L

E

F

Y

D

L

L

Y

Y

L

S

C

A

D

D

E

E

L

I

L

F

M

V

C

T

G
|
G

T
|
T

A
|
A

A

A

E

E

I

I

T

A

P

P

I

V

R

T

S

Y

V

I

D

D

G

G

K

R

K

T

I

V

G

G

S

N

G

G

K

K

A

P

G

G

R

K

V

V