SitesBLAST
Comparing N515DRAFT_2015 N515DRAFT_2015 branched-chain amino acid aminotransferase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
58% identity, 98% coverage: 7:305/305 of query aligns to 3:303/304 of 1iyeA
- active site: F33 (= F37), G35 (= G39), K156 (= K158), A157 (= A159), E190 (= E192), L214 (= L216)
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R60), Y92 (= Y96), Y126 (= Y128), K156 (= K158), Y161 (= Y163), E190 (= E192), G193 (= G195), E194 (= E196), N195 (= N197), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256), A255 (= A257)
1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
58% identity, 98% coverage: 7:305/305 of query aligns to 3:303/304 of 1iydA
- active site: F33 (= F37), G35 (= G39), K156 (= K158), A157 (= A159), E190 (= E192), L214 (= L216)
- binding glutaric acid: Y92 (= Y96), Y126 (= Y128), A255 (= A257)
- binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K158), Y161 (= Y163), E190 (= E192), G193 (= G195), E194 (= E196), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), T254 (= T256)
1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
58% identity, 98% coverage: 7:305/305 of query aligns to 3:303/304 of 1i1mA
- active site: K156 (= K158)
- binding 4-methyl valeric acid: Y92 (= Y96), K156 (= K158), T254 (= T256), A255 (= A257)
- binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K158), Y161 (= Y163), E190 (= E192), G193 (= G195), E194 (= E196), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256)
1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
58% identity, 98% coverage: 7:305/305 of query aligns to 3:303/304 of 1i1lA
- active site: K156 (= K158)
- binding 2-methylleucine: Y92 (= Y96), K156 (= K158), T254 (= T256), A255 (= A257)
- binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K158), Y161 (= Y163), E190 (= E192), G193 (= G195), G216 (= G218), I217 (= I219), T218 (= T220), T254 (= T256)
4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
48% identity, 97% coverage: 9:305/305 of query aligns to 9:306/306 of 4whxA
2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
44% identity, 96% coverage: 9:302/305 of query aligns to 7:301/305 of 2ej0B
- active site: F35 (= F37), G37 (= G39), K158 (= K158), E192 (= E192), L215 (= L216)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R60), Y163 (= Y163), E192 (= E192), G195 (= G195), E196 (= E196), L215 (= L216), G217 (= G218), I218 (= I219), T219 (= T220), G254 (= G255), T255 (= T256)
2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
43% identity, 96% coverage: 9:302/305 of query aligns to 7:293/297 of 2ej3A
- active site: F35 (= F37), G37 (= G39), K150 (= K158), E184 (= E192), L207 (= L216)
- binding [1-(aminomethyl)cyclohexyl]acetic acid: G187 (= G195), G246 (= G255), T247 (= T256), A248 (= A257)
- binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K158), Y155 (= Y163), E184 (= E192), G187 (= G195), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), G246 (= G255), T247 (= T256)
2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
43% identity, 96% coverage: 9:302/305 of query aligns to 7:293/297 of 2eiyA
- active site: F35 (= F37), G37 (= G39), K150 (= K158), E184 (= E192), L207 (= L216)
- binding 4-methyl valeric acid: F35 (= F37), Y94 (= Y96), T247 (= T256), A248 (= A257)
- binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K158), Y155 (= Y163), E184 (= E192), G187 (= G195), E188 (= E196), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), G246 (= G255), T247 (= T256)
1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
43% identity, 96% coverage: 9:302/305 of query aligns to 7:293/297 of 1wrvA
- active site: F35 (= F37), G37 (= G39), K150 (= K158), E184 (= E192), L207 (= L216)
- binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K158), Y155 (= Y163), E184 (= E192), G187 (= G195), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), T247 (= T256)
2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
43% identity, 96% coverage: 9:302/305 of query aligns to 7:290/294 of 2ej2A
- active site: F35 (= F37), G37 (= G39), K147 (= K158), E181 (= E192), L204 (= L216)
- binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: R58 (= R60), Y94 (= Y96), Y152 (= Y163), E181 (= E192), G184 (= G195), E185 (= E196), L204 (= L216), G206 (= G218), I207 (= I219), T208 (= T220), T244 (= T256), A245 (= A257)
6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
40% identity, 97% coverage: 9:305/305 of query aligns to 9:300/301 of 6thqB
- active site: F37 (= F37), K156 (= K158), E190 (= E192), L214 (= L216)
- binding pyridoxal-5'-phosphate: R60 (= R60), K156 (= K158), Y161 (= Y163), E190 (= E192), N195 (= N197), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), T254 (= T256)
- binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R60), Y97 (= Y96), K156 (= K158), Y161 (= Y163), E190 (= E192), G193 (= G195), E194 (= E196), N195 (= N197), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256), A255 (= A257)
6h65C Crystal structure of the branched-chain-amino-acid aminotransferase from haliangium ochraceum
36% identity, 98% coverage: 6:305/305 of query aligns to 4:306/308 of 6h65C
- active site: F35 (= F37), K158 (= K158), E192 (= E192), L216 (= L216)
- binding pyridoxal-5'-phosphate: R60 (= R60), K158 (= K158), Y163 (= Y163), E192 (= E192), A196 (≠ E196), L216 (= L216), S218 (≠ G218), V219 (≠ I219), T220 (= T220), G256 (= G255), T257 (= T256)
5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
37% identity, 93% coverage: 8:290/305 of query aligns to 3:280/290 of 5mr0D
- active site: F32 (= F37), G34 (= G39), K150 (= K158), E183 (= E192), L206 (= L216)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R60), G100 (= G109), L101 (= L110), K150 (= K158), Y154 (= Y163), E183 (= E192), G186 (= G195), D187 (≠ E196), L206 (= L216), I209 (= I219), T210 (= T220), G245 (= G255), T246 (= T256)
5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
37% identity, 91% coverage: 9:287/305 of query aligns to 5:278/290 of 5e25A
- active site: F33 (= F37), G35 (= G39), K151 (= K158), E184 (= E192), L207 (= L216)
- binding 2-oxoglutaric acid: Y88 (= Y96), K151 (= K158), T247 (= T256), A248 (= A257)
- binding pyridoxal-5'-phosphate: R52 (= R60), K151 (= K158), Y155 (= Y163), E184 (= E192), G187 (= G195), D188 (≠ E196), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), G246 (= G255), T247 (= T256)
6q8eA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum in pmp-form (see paper)
36% identity, 98% coverage: 6:305/305 of query aligns to 3:304/307 of 6q8eA
- active site: F34 (= F37), K156 (= K158), E190 (= E192), L214 (= L216)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R59 (= R60), K156 (= K158), Y161 (= Y163), E190 (= E192), G193 (= G195), S194 (≠ E196), C195 (≠ N197), L214 (= L216), S216 (≠ G218), I217 (= I219), T218 (= T220), G254 (= G255), T255 (= T256)
7neaA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum (m3 mutant). (see paper)
35% identity, 98% coverage: 6:305/305 of query aligns to 3:304/309 of 7neaA
- active site: F34 (= F37), K156 (= K158), E190 (= E192), L214 (= L216)
- binding pyridoxal-5'-phosphate: R59 (= R60), K156 (= K158), Y161 (= Y163), E190 (= E192), G193 (= G195), S194 (≠ E196), L214 (= L216), S216 (≠ G218), I217 (= I219), T218 (= T220), T255 (= T256)
6fteB Crystal structure of an (r)-selective amine transaminase from exophiala xenobiotica
29% identity, 92% coverage: 10:290/305 of query aligns to 31:308/321 of 6fteB
- active site: Y58 (≠ F37), K179 (= K158), E212 (= E192), L234 (= L216)
- binding pyridoxal-5'-phosphate: R77 (= R60), K179 (= K158), E212 (= E192), F216 (≠ E196), N217 (= N197), L234 (= L216), G236 (= G218), V237 (≠ I219), T238 (= T220), T274 (= T256)
3daaA Crystallographic structure of d-amino acid aminotransferase inactivated by pyridoxyl-d-alanine (see paper)
29% identity, 91% coverage: 9:287/305 of query aligns to 4:272/277 of 3daaA
- active site: Y31 (≠ F37), V33 (≠ G39), K145 (≠ P154), E177 (= E192), L201 (= L216)
- binding n-(5'-phosphopyridoxyl)-d-alanine: Y31 (≠ F37), R50 (= R60), K145 (≠ P154), E177 (= E192), S180 (≠ G195), S181 (≠ E196), L201 (= L216), G203 (= G218), I204 (= I219), T205 (= T220), S240 (≠ G255), T241 (= T256)
2daaA Crystallographic structure of d-amino acid aminotransferase inactivated by d-cycloserine
29% identity, 91% coverage: 9:287/305 of query aligns to 4:272/277 of 2daaA
- active site: Y31 (≠ F37), V33 (≠ G39), K145 (≠ P154), E177 (= E192), L201 (= L216)
- binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: Y31 (≠ F37), V33 (≠ G39), R50 (= R60), R98 (vs. gap), H100 (vs. gap), K145 (≠ P154), E177 (= E192), S180 (≠ G195), S181 (≠ E196), N182 (= N197), L201 (= L216), G203 (= G218), I204 (= I219), T205 (= T220), T241 (= T256)
1daaA Crystallographic structure of d-amino acid aminotransferase complexed with pyridoxal-5'-phosphate (see paper)
29% identity, 91% coverage: 9:287/305 of query aligns to 4:272/277 of 1daaA
- active site: Y31 (≠ F37), V33 (≠ G39), K145 (≠ P154), E177 (= E192), L201 (= L216)
- binding pyridoxal-5'-phosphate: R50 (= R60), K145 (≠ P154), E177 (= E192), S180 (≠ G195), S181 (≠ E196), L201 (= L216), G203 (= G218), I204 (= I219), T205 (= T220), S240 (≠ G255), T241 (= T256)
Query Sequence
>N515DRAFT_2015 N515DRAFT_2015 branched-chain amino acid aminotransferase
MAQQYPEWIWQNGQIKPWREATTHVMSHALHYGSSVFEGIRSYATPDGAAIFRLTDHLKR
LYQSAKIYDMVLPYSQDQIAAACRDVIKQNGLGAAYLRPVAYRGLGGFGLSAETPIDVAV
AAWPMGPYLGPEALESGIDACVSSWQRFAPNTIPAGAKAGGNYLSGQLIAREARRLGFGE
GIALANTGLLSEGAGENLFLVFDGVLHTTPASASILTGITRHTLITLAREDGIEVIERDI
PREYLYLCDELLMCGTAAEITPIRSVDGKKIGSGKAGRVTRRMQELFFGLFNGKTNDQWG
WLEPV
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory