SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing N515DRAFT_2085 FitnessBrowser__Dyella79:N515DRAFT_2085 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 18 hits to proteins with known functional sites (download)

P53322 High-affinity nicotinic acid transporter; Nicotinic acid permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
26% identity, 75% coverage: 1:326/434 of query aligns to 73:397/534 of P53322

query
sites
P53322

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K283 (≠ A216) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

P0AA76 D-galactonate transporter; D-galactonate/H(+) symporter from Escherichia coli (strain K12) (see paper)
23% identity, 90% coverage: 24:414/434 of query aligns to 18:414/430 of P0AA76

query
sites
P0AA76

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Y29 (= Y35) binding D-galactonate
D31 (= D37) mutation to N: Loss of galactonate transport activity.
R32 (= R38) binding D-galactonate
Y64 (= Y70) binding D-galactonate
E118 (= E124) mutation to Q: Loss of galactonate transport activity.
W358 (= W358) binding D-galactonate

8u3hA Structure of fmoc-leu-oh bound sialin (see paper)
20% identity, 91% coverage: 28:424/434 of query aligns to 12:408/425 of 8u3hA

query
sites
8u3hA

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binding leucine: M279 (= M298), S344 (≠ T359)
binding Fluorenylmethyloxycarbonyl chloride: Y19 (= Y35), H235 (≠ Y254), Y238 (≠ C257), Y243 (= Y262)

8u3gA Structure of naag-bound sialin (see paper)
20% identity, 91% coverage: 28:424/434 of query aligns to 12:410/427 of 8u3gA

query
sites
8u3gA

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E

A

G

G

V

F

T

F
|
F

P

P

G

A

I

M

V

H

L

A

Y

M

L

W

T

S

Y

S

W

W

Y

A

P

P

S

P

A

L

R

E

R

R

G

S

R

K

M

L

N

L

A

S

L

I

F

S

M

Y

T

A

G

G

I

A

P

Q

I

L

A

G

G

T

V

V

L

I

G

S

G

L

P

P

L

L

S

S

G

G

W

I

I

I

M

C

H

Y

A

-

F

-

G

-

V

-

H

-

G

-

L

Y

A

M

N

N

W

W

Q

T

W

Y

L

V

F

F

L

Y

L

F

E

F

A

G

L

T

P

I

S

G

L

I

A

F

L

W

G

F

I

L

A

L

T

W

P

I

F

W

V

L

L

V

P

S

N

D

G

T

I

P

R

Q

S

K

A

H

S

K

W

R

L

I

D

S

A

H

R

Y

E

E

K

K

Q

E

L

Y

L

I

E

L

D

S

R

S

L

L

A

R

G

N

D

Q

P

L

Q

S

A

S

G

Q

S

K

E

S

A

V

S

P

L

W

R

V

S

P

V

I

M

L

A

K

D

S

V

L

R

P

V

L

W

W

R

A

L

I

A

V

I

A

Y

H

F

F

C

S

C

Y

M

N

M

W

G

T

L

F

Y

Y

G

T

V

L

S

L

F

T

Y

L

L

L

P

P

T

T

L

Y

I

M

A

K

A

E

A

I

G

L

V

R

D

F

D

N

A

V

L

Q

D

E

V

N

G

G

L

F

L

L

T

S

A

S

I

L

P

P

Y

Y

A

L

V

G

A

S

V

W

V

L

S

C

M

M

I

I

L

L

L

S

A

G

R

Q

S

A

D

D

R

-

H

-

N

N

E

L

R

R

-

A

R

K

W

W

H

N

L

F

A

S

A

T

-

L

-

C

-

V

-

R

-

I

-

F

-

S

-

L

-

I

A

G

S

M

I

I

A

G

G

P

A

A

A

V

G

F

L

L

Y

V

A

A

S

A

T

G

L

F

C

I

G

G

S

C

E

D

L

Y

V

S

L

L

G

A

L

V

I

A

A

F

L

L

S

T

L

I

G

S

T

T

A

-

G

-

V

-

L

-

S

-

T

T

M

L

P

G

V

G

F

F

W

C

T

S

W

S

P

G

S

F

S
|
S

V

I

-

N

-
x
H

-

L

-

D

L

I

A

A

G

P

T

S

A

Y

A

A

A

G

A

I

G

L

I
x
L

A

G

M

I

I

T

N

N

S

T

I

F

G

A

N

T

L

I

A

P

G

G

F

M

V

V

S

G

P

P

S

V

I

I

I

-

G

-

W

-

M

A

K

K

D

S

L

L

T

T

H

P

S

D

T

N

N

T

A

V

G

G

L

E

W

W

V

-

A

Q

V

T

A

V

L

F

L

Y

L

I

G

A

A

A

L

I

A

N

L

V

L

F

G

G

S

A

binding aspartic acid: F15 (≠ Y31), Y19 (= Y35), F118 (= F128)
binding glutamic acid: S350 (= S363), H353 (vs. gap), L365 (≠ I374)

6e9nA E. Coli d-galactonate:proton symporter in the inward open form (see paper)
23% identity, 90% coverage: 24:414/434 of query aligns to 7:395/409 of 6e9nA

query
sites
6e9nA

L

L

P

V

F

M

L

I

F

F

I

I

C

T

Y

V

V

V

A

I

A

C

Y

Y

L

V

D

D

R

R

V

A

N

N

I

L

G

A

F

V

A

A

K

S

L

A

Q

H

M

I

L

Q

S

E

D

E

L

F

H

G

F

I

S

T

E

K

A

A

V

E

Y

M

G

G

L

Y

G

V

A

F

G

S

V

A

F

F

F

A

I

W

G

L

Y

Y

F

T

L

L

F

C

E

Q

V

I

P

P

S

G

N

G

M

W

L

F

L

L

H

D

R

R

L

V

G

G

A

S

R

R

L

V

W

T

I

Y

S

F

R

I

I

A

M

I

V

F

S

G

W

W

A

S

L

V

V

A

S

T

A

L

A

F

T

Q

M

G

L

F

V

A

T

T

T

G

P

L

T

M

A

S

F

L

Y

I

V

G

L

L

R

R

F

A

L

I

L

T

G

G

V

I

A

F

E

E

A

A

G

P

F

A

F
|
F

P

P

G

T

I

N

V

N

L

R

Y

M

L

V

T

T

Y

S

W

W

Y

F

P

P

S

E

A

H

R

E

R

R

G

A

R

S

M

A

N

V

A

G

L

F

F

Y

M

T

T

S

G

G

I

Q

P

F

I

V

A

G

G

L

V

A

L

F

G

L

G

T

P

P

L

L

S

L

G

I

W

W

I

I

M

Q

H

E

A

-

F

-

G

-

V

-

H

-

G

-

L

M

A

L

N

S

W

W

Q

H

W

W

L

V

F

F

L

I

L

V

E

T

A

G

L

G

P

I

S

G

L

I

A

I

-

W

-

S

-

L

-

I

-

W

L

F

G

K

I

V

A

Y

T

Q

P

P

F

P

V

R

L

L

P

T

N

K

G

G

I

I

R

S

S

K

A

A

S

E

W

L

L

D

D

Y

A

I

R

R

E

D

K

G

Q

G

L

G

L

L

E

V

D

D

R

-

L

-

A

-

G

G

D

D

-

A

P

P

Q

L

A

T

G

A

S

K

E

D

A

W

S

K

L

L

R

-

S

-

V

-

M

-

A

-

D

-

V

V

R

F

V

H

W

R

R

K

L

L

A

I

A

G

I

V

Y

Y

-

L

-

G

-

Q

F

F

C

A

C

V

M

A

M

S

G

T

L

L

Y

W

G

F

V

F

S

L

F

T

Y

W

L

F

P

P

T

N

L

Y

I

L

A

T

A

Q

A

E

G

K

V

G

D

I

D

T

A

A

L

L

D

K

V

A

G

G

L

F

L

M

T

T

A

T

I

V

P

P

Y

F

A

L

V

A

A

A

V

F

V

V

S

G

M

V

I

L

L

L

L

S

A

G

R

W

S

V

S

A

D

D

R

L

H

L

N

V

E

R

R

K

R

G

W

F

H

S

L

L

A

G

-

F

A

A

A

R

S

K

I

T

A

P

G

I

A

I

A

C

G

G

L

L

Y

L

A

I

S

S

T

T

-

C

L

I

C

M

G

G

S

A

E

N

-

Y

-

T

-

N

-

D

-

P

-

M

L

M

V

I

L

M

G

C

L

L

I

M

A

A

L

L

S

A

L

F

G

F

T

G

A

N

G

G

V

F

L

A

S

S

T

-

M

-

P

-

V

I

F

T

W

W

T

S

W

L

P

V

S

S

V

L

L

A

A

P

G

M

T

R

A

L

A

I

A

G

A

L

G

T

I

G

A

G

M

V

I

F

N
|
N

S

F

I

A

G

G

N

G

L

L

A

G

G

G

F

I

V

T

S

V

P

P

S

L

I

V

G

G

W

Y

M

L

K

A

D

Q

L

G

T

Y

H

G

S

F

T

A

N

P

A

A

G

L

L

V

W

Y

V

I

V

S

A

A

V

V

A

A

L

L

L

I

binding D-gluconic acid: F111 (= F128), N359 (= N378)

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
20% identity, 83% coverage: 64:424/434 of query aligns to 113:471/495 of Q9NRA2

query
sites
Q9NRA2

G

G

V

S

F

F

I

Y

G

G

Y

Y

F

I

L

I

F

T

E

Q

V

I

P

P

S

G

N

G

M

Y

L

V

L

A

H

S

R

K

L

I

G

G

A

G

R
x
K

L

M

W

-

I

-

S

-

R

-

I

-

M

L

V

L

S

G

W

F

A

G

L

I

V

L

S

G

A

T

A

A

T

V

M

L

L

T

V

L

T

F

T

T

P

P

T

I

A

A

-

A

-

D

-

L

-

G

-

V

-

G

-

P

F

L

Y

I

V

V

L

L

R

R

F

A

L

L

L

E

G

G

V

L

A

G

E

E

A

G

G

V

F

T

F

F

P

P

G

A

I

M

V
x
H

L

A

Y

M

L

W

T

S

Y

S

W

W

Y

A

P

P

S

P

A

L

R

E

R

R

G

S

R

K

M
x
L

N
x
L

A

S

L

I

F

S

M

Y

T

A

G

G

I

A

P

Q

I

L

A

G

G

T

V

V

L

I

G

S

G

L

P

P

L

L

S

S

G

G

W

I

I

I

M

C

H

Y

A

-

F

-

G

-

V

-

H

-

G

-

L

Y

A

M

N

N

W

W

Q

T

W

Y

L

V

F

F

L

Y

L

F

E

F

A

G

L

T

P

I

S

G

L

I

A

F

L

W

G

F

I

L

A

L

T

W

P

I

F

W

V

L

L

V

P

S

N

D

G

T

I

P

R

Q

S

K

A

H

S

K

W

R

L

I

D

S

A

H

R

Y

E

E

K

K

Q

E

L

Y

L
x
I

E
x
L

D
x
S

R
x
S

L
|
L

A
x
R

G
x
N

D

Q

P

L

Q

S

A

S

G

Q

S

K

E

S

A

V

S

P

L

W

R

V

S

P

V

I

M

L

A

K

D

S

V

L

R

P

V

L

W

W

R

A

L

I

A

V

I

A

Y

H

F

F

C

S

C

Y

M

N

M

W

G

T

L

F

Y

Y

G

T

V

L

S

L

F

T

Y

L

L

L

P

P

T

T

L

Y

I

M

A

K

A

E

A

I

G

L

V

R

D

F

D

N

A

V

L

Q

D

E

V

N

G
|
G

L

F

L

L

T

S

A

S

I

L

P
|
P

Y

Y

A

L

V

G

A

S

V

W

V

L

S

C

M

M

I

I

L

L

L

S

A

G

R

Q

S

A

D

D

R

-

H

-

N

N

E

L

R

R

-

A

R

K

W

W

H

N

L

F

A

S

A

T

-

L

-

C

-

V

-

R

-

I

-

F

-

S

-

L

-

I

A
x
G

S

M

I

I

A

G

G

P

A

A

A

V

G

F

L

L

Y

V

A

A

S

A

T

G

L

F

C

I

G

G

S

C

E

D

L

Y

V

S

L

L

G

A

L

V

I

A

A

F

L

L

S

T

L

I

G

S

T

T

A

-

G

-

V

-

L

-

S

-

T

T

M

L

P

G

V

G

F

F

W

C

T

S

W

S

P

G

S

F

S

S

V

I

-

N

-

H

-

L

-

D

L

I

A

A

G

P

T

S

A

Y

A

A

A

G

A

I

G

L

I

L

A

G

M

I

I

T

N

N

S

T

I

F

G

A

N

T

L

I

A

P

G

G

F

M

V

V

S

G

P

P

S

V

I

I

I

-

G

-

W

-

M

A

K

K

D

S

L

L

T

T

H

P

S

D

T

N

N

T

A

V

G

G

L

E

W

W

V

-

A

Q

V

T

A

V

L

F

L

Y

L

I

G

A

A

A

L

I

A

N

L

V

L

F

G

G

S

A

K136 (≠ R87) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
H183 (≠ V132) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
LL 198:199 (≠ MN 147:148) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
IL 266:267 (≠ LE 222:223) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
SSLRN 268:272 (≠ DRLAG 224:228) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
G328 (= G284) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
P334 (= P290) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
G371 (≠ A318) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794

Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
22% identity, 59% coverage: 64:320/434 of query aligns to 113:363/495 of Q5Q0U0

query
sites
Q5Q0U0

G

G

V

S

F

F

I

Y

G

G

Y

Y

F

I

L

V

F

T

E

Q

V

I

P

P

S

G

N

G

M

Y

L

M

L

A

H

S

R

R

L

I

G

G

A

G

R
x
K

L

L

W

L

I

L

S

G

R

L

I

G

M

I

V

L

S

G

W

T

A

A

L

V

V

F

S

T

A

L

A

F

T

T

M

P

L

L

V

A

T

A

T

D

-

L

-

G

P

V

T

V

A

A

F

L

Y

I

V

V

L

L

R
|
R

F

A

L

L

L
x
E

G
|
G

V

L

A

G

E
|
E

A
x
G

G

V

F

T

F
|
F

P
|
P

G

A

I

M

V
x
H

L

A

Y

M

L
x
W

T

S

Y

S

W

W

Y

A

P

P

S

P

A

L

R

E

R

R

G

S

R

K

M

L

N

L

A

T

L

I

F

S

M

Y

T

A

G

G

I

A

P

Q

I

L

A

G

G

T

V

V

L

I

G

S

G

L

P

P

L

L

S

S

G

G

W

I

I

I

M

C

H

Y

A

-

F

-

G

-

V

-

H

-

G

-

L

Y

A

M

N

N

W

W

Q

T

W

Y

L

V

F

F

L

Y

L

L

E

F

A

G

L

L

P

V

S

G

L

I

A

V

L

W

G

F

I

I

A

L

T

W

P

M

F

W

V

I

L

V

P

S

N

D

G

T

I

P

R

E

S

T

A

H

S

K

W

T

L

I

D

S

A

H

R

Y

E

E

K

K

Q

E

L

Y

L

I

E

V

D
x
S

R
x
S

L
|
L

A
x
K

G
x
N

D

Q

P

L

Q

S

A

S

G

Q

S

K

E

V

A

V

S

P

L

W

R

V

S

S

V

I

M

L

A

K

D

S

V

L

R

P

V

L

W

W

R

A

L

I

A

V

I

A

Y

H

F

F

C

S

C

Y

M

N

M

W

G

T

L

F

Y

Y

G

T

V

L

S

L

F

T

Y

L

L

L

P

P

T

T

L

Y

I

M

A

K

A

E

A

I

G

L

V

R

D

F

D

N

A

V

L

Q

D

E

V

N

G

G

L

F

L

L

T

S

A

A

I

L

P
|
P

Y

Y

A

F

V

G

A

C

V

W

V

L

S

C

M

M

I

I

L

V

L

C

A

G

R

Q

S

A

D

D

R

-

H

Y

N

L

E

R

R

V

R

K

W

W

H

N

L

F

A

S

A

T

A

I

S

S

I

V

K136 (≠ R87) mutation to E: Markedly decreases H(+)-coupled sialic acid transporter activity.
R168 (= R117) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
E171 (≠ L120) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
G172 (= G121) mutation to C: Decreases protein levels and alters subcellular localization.
E175 (= E124) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
G176 (≠ A125) mutation to C: Decreases protein levels and alters subcellular localization.
F179 (= F128) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
P180 (= P129) mutation to C: Decreases protein levels and alters subcellular localization.
H183 (≠ V132) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
W186 (≠ L135) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
SSLKN 268:272 (≠ DRLAG 224:228) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
P334 (= P290) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.
371 G→V: Remains in the endoplasmic reticulum.

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
21% identity, 59% coverage: 64:320/434 of query aligns to 113:363/495 of Q8BN82

query
sites
Q8BN82

G

G

V

S

F

F

I

Y

G

G

Y

Y

F

I

L

V

F

T

E

Q

V

I

P

P

S

G

N

G

M

Y

L

I

L

A

H

S

R

R

L

V

G

G

A

G

R

K

L

L

W

L

I

L

S

G

R

L

I

G

M

I

V

L

S

G

W

T

A

S

L

V

V

F

S

T

A

L

A

F

T

T

M

P

L

L

V

A

T

A

T

D

-

L

-

G

P

V

T

V

A

T

F

L

Y

V

V

V

L

L

R

R

F

A

L

L

L

E

G

G

V

L

A

G

E

E

A

G

G

V

F

T

F

F

P

P

G

A

I

M

V
x
H

L

A

Y

M

L

W

T

S

Y

S

W

W

Y

A

P

P

S

P

A

L

R

E

R

R

G

S

R

K

M

L

N

L

A

T

L

I

F

S

M

Y

T

A

G

G

I

A

P

Q

I

L

A

G

G

T

V

V

L

I

G

S

G

L

P

P

L

L

S

S

G

G

W

I

I

I

M

C

H

Y

A

-

F

-

G

-

V

-

H

-

G

-

L

Y

A

M

N

N

W

W

Q

T

W

Y

L

V

F

F

L

Y

L

L

E

F

A

G

L

I

P

V

S

G

L

I

A

V

L

W

G

F

I

I

A

L

T

W

P

M

F

W

V

I

L

V

P

S

N

D

G

T

I

P

R

E

S

T

A

H

S

K

W

T

L

I

D

S

A

H

R

Y

E

E

K

K

Q

E

L

Y

L

I

E

V

D

S

R

S

L

L

A

K

G

N

D

Q

P

L

Q

S

A

S

G

Q

S

K

E

V

A

V

S

P

L

W

R

G

S

S

V

I

M

L

A

K

D

S

V

L

R

P

V

L

W

W

R

A

L

I

A

V

I

A

Y

H

F

F

C

S

C

Y

M

N

M

W

G

S

L

F

Y

Y

G

T

V

L

S

L

F

T

Y

L

L

L

P

P

T

T

L

Y

I

M

A

K

A

E

A

I

G

L

V

R

D

F

D

N

A

V

L

Q

D

E

V

N

G

G

L

F

L

L

T

S

A

A

I

L

P

P

Y

Y

A

F

V

G

A

C

V

W

V

L

S

C

M

M

I

I

L

L

L

C

A

G

R

Q

S

A

D

D

R

-

H

Y

N

L

E

R

R

V

R

K

W

W

H

N

L

F

A

S

A

T

A

I

S

S

I

V

H183 (≠ V132) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

6e9oA E. Coli d-galactonate:proton symporter mutant e133q in the outward substrate-bound form (see paper)
21% identity, 90% coverage: 24:414/434 of query aligns to 10:379/393 of 6e9oA

query
sites
6e9oA

L

L

P

V

F

M

L

I

F

F

I

I

C

T

Y

V

V

V

A

I

A

C

Y
|
Y

L

V

D

D

R

R

V

A

N

N

I

L

G

A

F

V

A

A

K

S

L

A

Q

H

M

I

L

Q

S

E

D

E

L

F

H

G

F

I

S

T

E

K

A

A

V

E

Y

M

G

G

L

Y

G

V

A

F

G

S

V

A

F

F

F

A

I

W

G

L

Y

Y

F

T

L

L

F

C

E

Q

V

I

P

P

S

G

N

G

M

W

L

F

L

L

H

D

R

R

L

V

G

G

A

S

R

R

L

V

W

T

I

Y

S

F

R

I

I

A

M

I

V

F

S

G

W

W

A

S

L

V

V

A

S

T

A

L

A

F

T

Q

M

G

L

F

V

A

T

T

T

G

P

L

T

M

A

S

F

L

Y

I

V

G

L

L

R

R

F

A

L

I

L

T

G

G

V

I

A

F

E

Q

A

A

G

P

F

A

F
|
F

P

P

G

T

I

N

V

N

L

R

Y

M

L

V

T

T

Y

S

W

W

Y

F

P

P

S

E

A

H

R

E

R

R

G

A

R

S

M

A

N

V

A

G

L

F

F

Y

M

T

T

S

G

G

I
x
Q

P

F

I

V

A

G

G

L

V

A

L

F

G

L

G

T

P

P

L

L

S

L

G

I

W

W

I

I

M

Q

H

E

A

-

F

-

G

-

V

-

H

-

G

-

L

M

A

L

N

S

W

W

Q

H

W

W

L

V

F

F

L

I

L

V

E

T

A

G

L

-

P

-

S

-

L

-

A

G

L

I

G

G

I

I

A

-

T

-

P

-

F

-

V

-

L

-

P

-

N

-

G

-

I

I

R

W

S

S

A

L

S

I

W

W

L

F

D

K

A

V

R

Y

E

Q

K

P

Q

P

L

R

L

L

E

T

D

K

R

G

L

I

A

-

G

-

D

-

P

-

Q

-

A

-

G

-

S

S

E

K

A

A

S

E

L

L

R

D

S

Y

V

I

-

R

-

D

-

G

-

L

M

V

A

L

D

T

V

A

R

K

V

D

W

W

R

K

L

L

A

-

I

V

Y

F

F

H

C

R

C

K

M

L

M

I

G

G

L

V

Y

Y

G

L

V

G

S
x
Q

F

F

Y

A

L

V

P

A

T

S

L

T

I

L

A

W

A

-

G

-

V

F

D

F

D

L

A

T

L

L

D

K

V

A

G

G

L

F

L

M

T

T

A

T

I

V

P

P

Y

F

A

L

V

A

A

A

V

F

V

V

S

G

M

V

I

L

L

L

L

S

A

G

R

W

S

V

S

A

D

D

R

L

H

L

N

V

E

R

R

K

R

G

W

F

H

S

L

L

A

G

-

F

A

A

A

R

S

K

I

T

A

P

G

I

A

I

A

C

G

G

L

L

Y

L

A

I

S

S

T

T

-

C

L

I

C

M

G

G

S

A

E

N

-

Y

-

T

-

N

-

D

-

P

-

M

L

M

V

I

L

M

G

C

L

L

I

M

A

A

L

L

S

A

L

F

G

F

T

G

A

N

G

G

V

F

L

A

S
|
S

T

-

M

-

P

-

V

I

F

T

W

W

T

S

W

L

P

V

S

S

V

L

L

A

A

P

G

M

T

R

A

L

A

I

A

G

A

L

G

T

I

G

A

G

M

V

I

F

N
|
N

S

F

I

A

G

G

N

G

L

L

A

G

G

G

F

I

V

T

S

V

P

P

S

L

I

V

G

G

W

Y

M

L

K

A

D

Q

L

G

T

Y

H

G

S

F

T

A

N

P

A

A

G

L

L

V

W

Y

V

I

V

S

A

A

V

V

A

A

L

L

L

I

binding D-galactonic acid: Y21 (= Y35), F114 (= F128), Q141 (≠ I155), Q228 (≠ S265), S320 (= S352), N343 (= N378)

Q9Y2C5 Probable small intestine urate exporter; Solute carrier family 17 member 4 from Homo sapiens (Human) (see 2 papers)
24% identity, 43% coverage: 115:300/434 of query aligns to 168:347/497 of Q9Y2C5

query
sites
Q9Y2C5

V

V

L

L

R

R

F

I

L

V

L

Q

G

G

V

I

A

A

E

Q

A

V

G

M

F

V

F

L

P

T

G

G

I

Q

V

Y

L

S

Y

I

L

W

T

V

Y

K

W

W

Y

A

P

P

S

P

A

L

R

E

R

R

G

S

R

Q

M

L

N

T

A

T

L

I

F

A

M

G

T

S

G

G

-

S

-

M

-

L

-

G

-

S

-

F

-

I

I

V

P

L

I

L

A

A

G

G

V

G

L

L

G

L

G

C

P

Q

L

T

S

I

G

G

W

W

-

P

-

Y

I

V

M

F

H

Y

A

I

F

F

G

G

V

I

H

G

G

C

L

A

A

C

N

C

W

P

Q

L

W

W

L

F

F

P

L

L

L

I

E

Y

A

D

L

D

P

P

S

-

L

-

A

-

L

-

G

-

I

V

A

N

T

H

P

P

F

F

V

I

L

-

P

-

N

-

G

-

I

-

R

-

S

-

A

-

S

-

W

-

L

-

D

S

A

A

R

G

E

E

K

K

Q

R

L

Y

L

I

E

V

D

C

R

S

L

L

A

A

G

Q

-

Q

D

D

P

C

Q

S

A

P

G

G

S

W

E

S

A

L

S

P

L

I

R

R

S

A

V

M

M

I

A

K

D

S

V

L

R

P

V

L

W

W

R

A

L

I

A

L

A

V

I

S

Y

Y

F

F

C

C

C

E

M

Y

M

W

G

L

L

F

Y

Y

G

T

V

I

S

M

F

A

Y

Y

L

T

P

P

T

T

L

Y

I

I

A

S

A

V

G

L

V

Q

D

A

D

N

A

L

L

R

D

D

V

S

G

G

L

I

L

L

T

S

A

A

I

L

P

P

Y

F

A

V

V

V

A

G

V

C

V

I

S

C

M

I

I

I

L

L

Sites not aligning to the query:

66 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
75 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-66 and A-90.
90 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
372 A → T: in dbSNP:rs11754288

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
24% identity, 82% coverage: 80:434/434 of query aligns to 80:442/452 of Q5EXK5

query
sites
Q5EXK5

L

L

L

S

H
x
D

R

R

L

F

G

G

A

R

R

K

L

R

W

V

I

I

S

E

R

F

I

C

M

V

V

A

S

L

W

F

A

G

L

L

V

F

S

S

A

L

T

S

M

A

L

F

V

A

T

P

T

D

P

L

T

Q

A

M

F

L

Y

V

V

F

L

L

R

R

F

F

L

L

L

T

G

G

V

L

A

G

E

L

A

G

G

G

F

A

F

M

P

P

G

N

I

T

V

I

L

T

Y

M

L

T

T

S

Y

E

W

Y

Y

L

P

P

S

A

A

R

R

R

G

G

R

A

M

L

N

V

A

T

L

L

F

M

M

F

T

C

G

G

I

F

P

T

I

L

A

G

G

S

V

A

L

F

G

G

P

V

L

V

S

S

G

A

W

Q

I

L

M

V

H

P

A

L

F

I

G

G

G

-

V

-

H

-

G

-

L

-

A

-

N

-

W

W

Q

H

W

G

L

I

F

L

L

V

L

L

E

G

A

G

L

V

P

L

S

P

L

L

A

I

L

L

G

A

I

V

A

A

T

L

P

V

F

P

V

L

L

L

P

P

N

E

G

-

I

-

R

-

S

S

A

P

S

R

W

W

L

Q

D

I

A

R

R

R

E

Q

-

L

K

P

Q

Q

L

A

L

T

E

I

D

A

R

R

L

T

A

V

G

G

-

A

-

I

-

T

-

G

-

E

-

R

-

Y

-

N

-

D

-

T

-

H

-

F

-

W

-

L

-

D

D

E

P

P

Q

A

A

A

G

G

S

A

E

K

A

G

S

S

L

I

R

S

S

Q

V

L

M

F

A

A

D

G

V

R

R

Q

V

L

-

A

-

I

-

T

W

L

R

M

L

L

A

W

A

V

I

V

Y

F

F

F

C

M

C

S

M

L

G

I

L

I

Y

Y

G

L

V

L

S

S

F

S

Y

W

L

M

P

P

T

T

L

L

I

L

A

N

A

H

A

R

G

G

V

I

D

D

D

L

A

Q

L

-

D

H

V

A

G

S

L

W

L

V

T

T

A

A

I

A

P

F

Y

Q

A

I

V

G

A

G

V

T

V

F

S

G

M

A

I

L

L

L

A

G

R
x
V

S

L

S

M

D
|
D

R

R

H

F

N

N

E

P

R

Y

R

R

W

-

H

V

L

L

A

A

A

L

A

S

S

Y

I

G

A

L

G

G

A

A

A

V

G

C

L

I

Y

V

A

M

S

I

T

G

L

L

C

-

G

-

S

S

E

E

L

N

V

G

L

L

G

W

L

L

I

M

A

A

L

L

S

A

L

I

G

F

T

G

A

T

G

G

V

I

-

G

L

I

S

S

T

G

M

S

P

Q

V

V

F

G

W

L

T

N

W

A

P

L

S

T

S

A

V

T

L

L

A

Y

G

P

T

T

A

Q

A

S

-

R

A

A

A

T

G

G

I

V

A

S

M

W

I

S

N

N

S

A

I

V

G

G

N

R

L

C

A

G

G

A

F

I

V

V

S

G

P

S

S

L

I

S

I

G

G

G

W

V

M

M

K

M

D

A

L

M

T

N

H

F

S

S

T

F

N

D

A

T

G

L

L

F

W

F

V

V

V

I

A

A

V

V

A

P

L

A

L

V

G

S

A

A

A

V

-

M

L

L

A

I

L

L

G

T

S

L

A

A

A

V

K

R

A

N

P

P

A

T

R

S

M

V

R

P

A

A

D82 (≠ H82) mutation to A: Loss of activity.
V311 (≠ R303) mutation to W: Loss of activity.
D314 (= D306) mutation to A: Loss of activity.

A0LNN5 L-lactate transporter; SfMCT from Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) (see paper)
24% identity, 84% coverage: 72:434/434 of query aligns to 62:412/412 of A0LNN5

query
sites
A0LNN5

L

L

F

M

E

T

V

F

P

P

S

A

N

G

M

R

L

L

L

S

H

D

R

K

L

M

G

G

A

P

R

R

L

-

W

-

I

-

S

K

R

V

I

V

M

M

V

T

S

G

W

G

A

V

L

L

V

L

S

A

A

I

A

G

T

F

M

I

L

L

V

S

T

G

T

F

P

I

T

Q

A

S

F

K

Y

Y

V

Q

L

L

R

Y

F

I

L

T

L

Y

G

G

V

V

A

I

E

A

A

G

G

-

F

-

F

F

P

G

G

G

I

G

V

M

L

I

Y
|
Y

L

L

T

P

-

P

-

I

-

A

-

T

-

A

-

P

Y

K

W

W

Y

W

P

P

S

D

A

-

R

R

G

A

R

L

M

A

N

T

A

G

L

F

F

A

M

V

T

V

G

G

I
x
L

P

G

I

L

A

G

G

S

V

F

L

L

G

M

G

G

P

P

L

L

S

A

G

T

W

Y

I

I

M

I

H

E

A

K

F

P

G

G

G

-

V

-

H

-

G

-

L

-

A

M

N

G

W

W

Q

R

W

Y

L

V

F

F

L

W

L

Y

-

C

-

G

E

V

A

A

L

M

P

G

S

I

L

M

A

A

L

L

G

-

I

I

A

A

T

G

P

A

F

F

V

L

L

E

P

P

N

P

G

-

I

-

R

-

S

P

A

A

S

G

W

W

L

K

D

P

A

A

R

G

E

Y

K

T

Q

P

L

P

L

A

E

P

D

P

R

A

L

G

A

A

G

A

D

A

P

P

Q

K

A

V

G

T

S

R

E

D

A

W

S

T

L

Y

R

E

S

E

V

A

M

K

A

G

D

D

V

T

R

K

V

F

W

W

R

L

L

L

A

Y

A

L

I

A

Y

Y

F

F

C

C

C

G

M

S

M

F

G

A

L

G

Y

L

G

M

V

V

S

I

F

G

Y
x
H

L

L

P

A

T

G

L

F

I

G

A
x
R

A
x
D

A

A

G

G

V

L

D

T

D

A

A

M

L

A

D

A

V

A

G

G

L

A

L

V

T

S

A

S

I

L

P

A

Y

F

A

S

V
x
N

A

A

V

A

V

-

S

T

M
x
R

I

I

L

L

L

S

A

G

R

W

S

F

S

V

D

D

R

K

H

I

N

G

E

I

R

R

R

V

W

Y

H

F

L

A

A

A

A

L

A

F

S

A

I

L

A

Q

G

T

A

A

G

M

L

I

Y

A

A

I

S

F

T

Q

L

L

C

G

G

G

S

S

E

V

L

V

V

G

L

L

G

S

L

I

I

V

A

A

L

I

S

V

L

I

G

G

-

W

T

N

A
x
Y

G

G

V

A

L

M

S
x
F

T

T

M

L

P

-

V

-

F

-

W

-

T

-

W

F

P

P

S

A

S

T

V

C

L

L

-

Q

-

F

-

Y

A

G

G

P

T

T

A

A

A

Q

A

G

A

S

G

N

I

Y

A

G

M

L

I

L

N
x
F

S

T

I

A

G
x
C

N

G

L

L

A

A

G

G

F

F

V

A

S

G

P

P

S

W

I

V

I

G

G

G

W

W

M

L

K
|
K

D
|
D

L

T

T

T

H

G

S

T

T
x
Y

N

Y

A

-

G

-

L

-

W

-

V

-

A

-

V

L

A

P

L

F

L

L

L

C

G

A

A

A

L

L

A

C

L

A

L

L

G

G

S

T

A

A

-

I

-

V

-

F

-

M

A

T

K

K

A

P

P

P

A

E

R

K

M

K

R

H

A

A

Y119 (= Y134) binding (S)-lactate; mutation Y->A,F: Loss of transport activity.
L145 (≠ I155) mutation to A: Strong decrease in transport activity.
H250 (≠ Y267) mutation to A: Strong decrease in transport activity.; mutation to F: Loss of transport activity.
R256 (≠ A273) mutation to A: No change in transport activity.; mutation to D: Increases transport activity.
D257 (≠ A274) mutation to A: Loss of transport activity.
N276 (≠ V293) mutation to A: Loss of transport activity.
R280 (≠ M298) binding (S)-lactate; mutation to A: Abolishes L-lactate binding and L-lactate transport.
Y331 (≠ A348) mutation to A: Loss of transport activity.; mutation to F: No change in transport activity.
F335 (≠ S352) mutation to A: Increases transport activity.
F359 (≠ N378) mutation to A: Loss of transport activity.
C362 (≠ G381) mutation to A: Decrease in transport activity.
K377 (= K396) mutation K->A,D: No change in transport activity.
D378 (= D397) mutation to A: Loss of transport activity.
Y383 (≠ T402) mutation to A: Loss of transport activity.; mutation to F: Strong decrease in transport activity.

Sites not aligning to the query:

28 L→A: Loss of transport activity.

6zguA Crystal structure of a mfs transporter with bound 3-(2-methylphenyl) propanoic acid at 2.41 angstroem resolution
24% identity, 84% coverage: 72:434/434 of query aligns to 56:396/404 of 6zguA

query
sites
6zguA

L

L

F

M

E

T

V

F

P

P

S

A

N

G

M

R

L

L

L

S

H

D

R

K

L

M

G

G

A

P

R

R

L

-

W

-

I

-

S

K

R

V

I

V

M

M

V

T

S

G

W

G

A

V

L

L

V

L

S

A

A

I

A

G

T

F

M

I

L

L

V

S

T

G

T

F

P

I

T

Q

A

S

F

K

Y

Y

V

Q

L

L

R

Y

F

I

L

T

L

Y

G

G

V

V

A

I

E

A

A

G

G

-

F

-

F

F

P

G

G

G

I

G

V

M

L

I

Y
|
Y

L

L

T

P

-

P

-

I

-

A

-

T

-

A

-

P

Y

K

W

W

Y

W

P

P

S

D

A

-

R

R

G

A

R

L

M

A

N

T

A

G

L

F

F

A

M

V

T

V

G

G

I
x
L

P

G

I

L

A

G

G

S

V

F

L

L

G

M

G

G

P

P

L

L

S

A

G

T

W

Y

I

I

M

I

H

E

A

K

F

P

G

G

G

-

V

-

H

-

G

-

L

-

A

M

N

G

W

W

Q

R

W

Y

L

V

F

F

L

W

L

Y

-

C

-

G

E

V

A

A

L

M

P

G

S

I

L

M

A

A

L

L

G

-

I

I

A

A

T

G

P

A

F

F

V

L

L

E

P

P

N

P

G

-

I

-

R

-

S

P

A

A

S

G

W

W

L

K

D

P

A

A

R

G

E

Y

K

-

Q

-

L

-

E

-

D

-

R

-

L

-

A

-

G

-

D

T

P

P

Q

K

A

V

G

T

S

R

E

D

A

W

S

T

L

Y

R

E

S

E

V

A

M

K

A

G

D

D

V

T

R

K

V

F

W

W

R

L

L

L

A

Y

A

L

I

A

Y

Y

F

F

C

C

C

G

M

S

M

F

G

A

L

G

Y

L

G

M

V

V

S

I

F

G

Y

H

L

L

P

A

T

G

L

F

I

G

A

R

A

D

A

A

G

G

V

L

D

T

D

A

A

M

L

A

D

A

V

A

G

G

L

A

L

V

T

S

A

S

I

L

P

A

Y

F

A

S

V

N

A

A

V

A

V

-

S

T

M
x
R

I

I

L

L

L

S

A

G

R

W

S

F

S

V

D

D

R

K

H

I

N

G

E

I

R

R

R

V

W

Y

H

F

L

A

A

A

A

L

A

F

S

A

I

L

A

Q

G

T

A

A

G

M

L

I

Y

A

A

I

S

F

T

Q

L

L

C

G

G

G

S

S

E

V

L

V

V

G

L

L

G

S

L

I

I

V

A

A

L

I

S

V

L

I

G

G

-

W

T

N

A

Y

G

G

V

A

L

M

S
x
F

T

T

M

L

P

-

V

-

F

-

W

-

T

-

W

F

P

P

S

A

S

T

V

C

L

L

-

Q

-

F

-

Y

A

G

G

P

T

T

A

A

A

Q

A

G

A

S

G

N

I

Y

A

G

M

L

I

L

N

F

S

T

I

A

G
x
C

N

G

L

L

A

A

G

G

F

F

V

A

S

G

P

P

S

W

I

V

I

G

G

G

W

W

M

L

K

K

D

D

L

T

T

T

H

G

S

T

T

Y

N

Y

A

-

G

-

L

-

W

-

V

-

A

-

V

L

A

P

L

F

L

L

L

C

G

A

A

A

L

L

A

C

L

A

L

L

G

G

S

T

A

A

-

I

-

V

-

F

-

M

A

T

K

K

A

P

P

P

A

E

R

K

M

K

R

H

A

A

binding 3-(2-methylphenyl)propanoic acid: Y113 (= Y134), L139 (≠ I155), R264 (≠ M298), F319 (≠ S352), C346 (≠ G381)

Sites not aligning to the query:

binding 3-(2-methylphenyl)propanoic acid: 54

6zgtA Crystal structure of a mfs transporter with bound 2-naphthoic acid at 2.39 angstroem resolution
24% identity, 84% coverage: 72:434/434 of query aligns to 56:396/404 of 6zgtA

query
sites
6zgtA

L

L

F

M

E

T

V

F

P

P

S

A

N

G

M

R

L

L

L

S

H

D

R

K

L

M

G

G

A

P

R

R

L

-

W

-

I

-

S

K

R

V

I

V

M

M

V

T

S

G

W

G

A

V

L

L

V

L

S

A

A

I

A

G

T

F

M

I

L

L

V

S

T

G

T

F

P

I

T

Q

A

S

F

K

Y

Y

V

Q

L

L

R

Y

F

I

L

T

L

Y

G

G

V

V

A

I

E

A

A

G

G

-

F

-

F

F

P

G

G

G

I

G

V

M

L

I

Y
|
Y

L

L

T

P

-

P

-

I

-

A

-

T

-

A

-

P

Y

K

W

W

Y

W

P

P

S

D

A

-

R

R

G

A

R

L

M

A

N

T

A

G

L

F

F

A

M

V

T

V

G

G

I
x
L

P

G

I

L

A

G

G

S

V

F

L

L

G

M

G

G

P

P

L

L

S

A

G

T

W

Y

I

I

M

I

H

E

A

K

F

P

G

G

G

-

V

-

H

-

G

-

L

-

A

M

N

G

W

W

Q

R

W

Y

L

V

F

F

L

W

L

Y

-

C

-

G

E

V

A

A

L

M

P

G

S

I

L

M

A

A

L

L

G

-

I

I

A

A

T

G

P

A

F

F

V

L

L

E

P

P

N

P

G

-

I

-

R

-

S

P

A

A

S

G

W

W

L

K

D

P

A

A

R

G

E

Y

K

-

Q

-

L

-

E

-

D

-

R

-

L

-

A

-

G

-

D

T

P

P

Q

K

A

V

G

T

S

R

E

D

A

W

S

T

L

Y

R

E

S

E

V

A

M

K

A

G

D

D

V

T

R

K

V

F

W

W

R

L

L

L

A

Y

A

L

I

A

Y

Y

F

F

C

C

C

G

M

S

M

F

G

A

L

G

Y

L

G

M

V

V

S

I

F

G

Y

H

L

L

P

A

T

G

L

F

I

G

A

R

A

D

A

A

G

G

V

L

D

T

D

A

A

M

L

A

D

A

V

A

G

G

L

A

L

V

T

S

A

S

I

L

P

A

Y

F

A

S

V

N

A

A

V

A

V

-

S

T

M
x
R

I

I

L

L

L

S

A

G

R

W

S

F

S

V

D

D

R

K

H

I

N

G

E

I

R

R

R

V

W

Y

H

F

L

A

A

A

A

L

A

F

S

A

I

L

A

Q

G

T

A

A

G

M

L

I

Y

A

A

I

S

F

T

Q

L

L

C

G

G

G

S

S

E

V

L

V

V

G

L

L

G

S

L

I

I

V

A

A

L

I

S

V

L

I

G

G

-

W

T

N

A

Y

G

G

V

A

L

M

S
x
F

T

T

M

L

P

-

V

-

F

-

W

-

T

-

W

F

P

P

S

A

S

T

V

C

L

L

-

Q

-

F

-

Y

A

G

G

P

T

T

A

A

A

Q

A

G

A

S

G

N

I

Y

A

G

M

L

I

L

N
x
F

S

T

I

A

G
x
C

N

G

L

L

A

A

G

G

F

F

V

A

S

G

P

P

S

W

I

V

I

G

G

G

W

W

M

L

K

K

D

D

L

T

T

T

H

G

S

T

T

Y

N

Y

A

-

G

-

L

-

W

-

V

-

A

-

V

L

A

P

L

F

L

L

L

C

G

A

A

A

L

L

A

C

L

A

L

L

G

G

S

T

A

A

-

I

-

V

-

F

-

M

A

T

K

K

A

P

P

P

A

E

R

K

M

K

R

H

A

A

binding naphthalene-2-carboxylic acid: Y113 (= Y134), L139 (≠ I155), R264 (≠ M298), F319 (≠ S352), F343 (≠ N378), C346 (≠ G381)

Sites not aligning to the query:

binding naphthalene-2-carboxylic acid: 51, 54

6zgsA Crystal structure of a mfs transporter with bound 3-phenylpropanoic acid at 2.39 angstroem resolution
24% identity, 84% coverage: 72:434/434 of query aligns to 56:396/404 of 6zgsA

query
sites
6zgsA

L

L

F

M

E

T

V

F

P

P

S

A

N

G

M

R

L

L

L

S

H

D

R

K

L

M

G

G

A

P

R

R

L

-

W

-

I

-

S

K

R

V

I

V

M

M

V

T

S

G

W

G

A

V

L

L

V

L

S

A

A

I

A

G

T

F

M

I

L

L

V

S

T

G

T

F

P

I

T

Q

A

S

F

K

Y

Y

V

Q

L

L

R

Y

F

I

L

T

L

Y

G

G

V

V

A

I

E

A

A

G

G

-

F

-

F

F

P

G

G

G

I

G

V

M

L

I

Y
|
Y

L

L

T

P

-

P

-

I

-

A

-

T

-

A

-

P

Y

K

W

W

Y

W

P

P

S

D

A

-

R

R

G

A

R

L

M

A

N

T

A

G

L

F

F

A

M

V

T

V

G

G

I
x
L

P

G

I

L

A

G

G

S

V

F

L

L

G

M

G

G

P

P

L

L

S

A

G

T

W

Y

I

I

M

I

H

E

A

K

F

P

G

G

G

-

V

-

H

-

G

-

L

-

A

M

N

G

W

W

Q

R

W

Y

L

V

F

F

L

W

L

Y

-

C

-

G

E

V

A

A

L

M

P

G

S

I

L

M

A

A

L

L

G

-

I

I

A

A

T

G

P

A

F

F

V

L

L

E

P

P

N

P

G

-

I

-

R

-

S

P

A

A

S

G

W

W

L

K

D

P

A

A

R

G

E

Y

K

-

Q

-

L

-

E

-

D

-

R

-

L

-

A

-

G

-

D

T

P

P

Q

K

A

V

G

T

S

R

E

D

A

W

S

T

L

Y

R

E

S

E

V

A

M

K

A

G

D

D

V

T

R

K

V

F

W

W

R

L

L

L

A

Y

A

L

I

A

Y

Y

F

F

C

C

C

G

M

S

M

F

G

A

L

G

Y

L

G

M

V

V

S

I

F

G

Y

H

L

L

P

A

T

G

L

F

I

G

A

R

A

D

A

A

G

G

V

L

D

T

D

A

A

M

L

A

D

A

V

A

G

G

L

A

L

V

T

S

A

S

I

L

P

A

Y

F

A

S

V

N

A

A

V

A

V

-

S

T

M
x
R

I

I

L

L

L

S

A

G

R

W

S

F

S

V

D

D

R

K

H

I

N

G

E

I

R

R

R

V

W

Y

H

F

L

A

A

A

A

L

A

F

S

A

I

L

A

Q

G

T

A

A

G

M

L

I

Y

A

A

I

S

F

T

Q

L

L

C

G

G

G

S

S

E

V

L

V

V

G

L

L

G

S

L

I

I

V

A

A

L

I

S

V

L

I

G

G

-

W

T

N

A

Y

G

G

V

A

L

M

S
x
F

T

T

M

L

P

-

V

-

F

-

W

-

T

-

W

F

P

P

S

A

S

T

V

C

L

L

-

Q

-

F

-

Y

A

G

G

P

T

T

A

A

A

Q

A

G

A

S

G

N

I

Y

A

G

M

L

I

L

N

F

S

T

I

A

G
x
C

N

G

L

L

A

A

G

G

F

F

V

A

S

G

P

P

S

W

I

V

I

G

G

G

W

W

M

L

K

K

D

D

L

T

T

T

H

G

S

T

T

Y

N

Y

A

-

G

-

L

-

W

-

V

-

A

-

V

L

A

P

L

F

L

L

L

C

G

A

A

A

L

L

A

C

L

A

L

L

G

G

S

T

A

A

-

I

-

V

-

F

-

M

A

T

K

K

A

P

P

P

A

E

R

K

M

K

R

H

A

A

binding hydrocinnamic acid: Y113 (= Y134), L139 (≠ I155), R264 (≠ M298), F319 (≠ S352), C346 (≠ G381)

Sites not aligning to the query:

binding hydrocinnamic acid: 54

Q9C0U9 Uncharacterized transporter PB1C11.03 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
19% identity, 44% coverage: 46:238/434 of query aligns to 126:315/570 of Q9C0U9

query
sites
Q9C0U9

L

L

Q

G

M

L

L

F

S

D

D

D

L

V

H

H

F

I

S

T

E

S

A

N

V

Q

Y

Y

G

N

L

N

G

L

A

N

G

T

V

L

F

F

F

Y

I

V

G

G

Y

Y

F

I

L

V

F

G

E

Q

V

F

P

P

S

G

N

H

M

Y

L

I

L

M

H

Q

R

T

L

F

G

P

A

L

R

G

L

K

W

F

I

V

S

G

R

L

I

V

M

T

V

F

S

S

W

W

A

S

L

V

V

I

S

V

A

F

A

L

T

H

M

C

L

C

V

A

T

Y

T

N

P

Y

T

G

A

G

F

L

Y

I

V

A

L

L

R

R

F

F

L

F

L

L

G

G

V

F

A

T

E

E

A

S

G

C

F

L

P

P

G

A

I

M

V

E

L

A

Y

T

L

M

T

G

Y

M

W

F

Y

F

P

T

S

H

A

Q

R

E

R

Q

G

A

R

F

M

L

N

Q

A

P

L

V

F

F

M

W

T

-

G

-

I

-

P

-

I

I

A

S

G

C

V

L

L

S

G

C

G

G

P

I

L

P

S

A

G

G

W

F

I

I

M

A

H

Y

A

G

F

L

G

E

G

F

V

V

-

T

H

K

G

S

L

I

A

A

N

P

W

W

Q

K

W

L

L

F

F

M

L

I

E

T

A

G

L

G

P

I

S

T

L

F

A

F

L

L

G

S

I

I

A

F

T

L

P

F

F

F

V

Y

L

Y

P

P

N

D

G

N

I

P

R

S

S

K

A

A

S

R

W

F

L

L

D

T

A

D

R

E

E

E

K

K

Q

L

L

Y

L

T

E

I

D

D

R

R

L

V

A

R

G

K

D

S

P

T

Q

R

A

G

G

G

S

I

E

E

A

N

S

K

L

I

Sites not aligning to the query:

14 modified: Phosphoserine

6zgrA Crystal structure of a mfs transporter with bound 1- hydroxynaphthalene-2-carboxylic acid at 2.67 angstroem resolution
23% identity, 88% coverage: 51:434/434 of query aligns to 33:391/399 of 6zgrA

query
sites
6zgrA

D

N

L

A

H

E

F

F

S

S

E

R

A

A

V

E

Y

I

G

A

L

M

G

A

A

F

G

A

V

I

F

C

I

L

G

I

Y
x
F

F

G

L

L

F

M

E

T

V

F

P

P

S

A

N

G

M

R

L

L

L

S

H

D

R

K

L

M

G

G

A

P

R

R

L

-

W

-

I

-

S

K

R

V

I

V

M

M

V

T

S

G

W

G

A

V

L

L

V

L

S

A

A

I

A

G

T

F

M

I

L

L

V

S

T

G

T

F

P

I

T

Q

A

S

F

K

Y

Y

V

Q

L

L

R

Y

F

I

L

T

L

Y

G

G

V

V

A

I

E

A

A

G

G

-

F

-

F

F

P

G

G

G

I

G

V

M

L

I

Y
|
Y

L

L

T

P

-

P

-

I

-

A

-

T

-

A

-

P

Y

K

W

W

Y

W

P

P

S

D

A

-

R

R

G

A

R

L

M

A

N

T

A

G

L

F

F

A

M

V

T

V

G

G

I
x
L

P

G

I

L

A

G

G

S

V

F

L

L

G

M

G

G

P

P

L

L

S

A

G

T

W

Y

I

I

M

I

H

E

A

-

F

-

G

-

V

-

H

-

G

-

L

-

A

-

N

-

W

W

Q

R

W

Y

L

V

F

F

L

W

L

Y

-

C

-

G

E

V

A

A

L

M

P

G

S

I

L

M

A

A

L

L

G

-

I

I

A

A

T

G

P

A

F

F

V

L

L

E

P

P

N

P

G

-

I

-

R

-

S

P

A

A

S

G

W

W

L

K

D

P

A

A

R

G

E

Y

K

T

Q

A

L

-

E

-

D

-

R

-

L

-

A

-

G

-

D

A

P

P

Q

K

A

V

G

T

S

R

E

D

A

W

S

T

L

Y

R

E

S

E

V

A

M

K

A

G

D

D

V

T

R

K

V

F

W

W

R

L

L

L

A

Y

A

L

I

A

Y

Y

F

F

C

C

C

G

M

S

M

F

G

A

L

G

Y

L

G

M

V

V

S

I

F

G

Y

H

L

L

P

A

T

G

L

F

I

G

A

R

A

D

A

A

G

G

V

L

D

T

D

A

A

M

L

A

D

A

V

A

G

G

L

A

L

V

T

S

A

S

I

L

P

A

Y

F

A

S

V

N

A

A

V

A

V

-

S

T

M
x
R

I

I

L

L

L

S

A

G

R

W

S

F

S

V

D

D

R

K

H

I

N

G

E

I

R

R

R

V

W

Y

H

F

L

A

A

A

A

L

A

F

S

A

I

L

A

Q

G

T

A

A

G

M

L

I

Y

A

A

I

S

F

T

Q

L

L

C

G

G

G

S

S

E

V

L

V

V

G

L

L

G

S

L

I

I

V

A

A

L

I

S

V

L

I

G

G

-

W

T

N

A
x
Y

G

G

V

A

L

M

S
x
F

T

T

M

L

P

-

V

-

F

-

W

-

T

-

W

F

P

P

S

A

S

T

V

C

L

L

-

Q

-

F

-

Y

A

G

G

P

T

T

A

A

A

Q

A

G

A

S

G

N

I

Y

A

G

M

L

I

L

N
x
F

S

T

I

A

G

C

N

G

L

L

A

A

G

G

F

F

V

A

S

G

P

P

S

W

I

V

I

G

G

G

W

W

M

L

K

K

D

D

L

T

T

T

H

G

S

T

T

Y

N

Y

A

-

G

-

L

-

W

-

V

-

A

-

V

L

A

P

L

F

L

L

L

C

G

A

A

A

L

L

A

C

L

A

L

L

G

G

S

T

A

A

-

I

-

V

-

F

-

M

A

T

K

K

A

P

P

P

A

E

R

K

M

K

R

H

A

A

binding 1-hydroxynaphthalene-2-carboxylic acid: F52 (≠ Y70), Y111 (= Y134), L137 (≠ I155), R259 (≠ M298), Y310 (≠ A348), F314 (≠ S352), F338 (≠ N378)

6g9xA Crystal structure of a mfs transporter at 2.54 angstroem resolution (see paper)
24% identity, 84% coverage: 72:434/434 of query aligns to 53:388/396 of 6g9xA

query
sites
6g9xA

L

L

F

M

E

T

V

F

P

P

S

A

N

G

M

R

L

L

L

S

H

D

R

K

L

M

G

G

A

P

R

R

L

-

W

-

I

-

S

K

R

V

I

V

M

M

V

T

S

G

W

G

A

V

L

L

V

L

S

A

A

I

A

G

T

F

M

I

L

L

V

S

T

G

T

F

P

I

T

Q

A

S

F

K

Y

Y

V

Q

L

L

R

Y

F

I

L

T

L

Y

G

G

V

V

A

I

E

A

A

G

G

-

F

-

F

F

P

G

G

G

I

G

V

M

L

I

Y
|
Y

L

L

T

P

-

P

-

I

-

A

-

T

-

A

-

P

Y

K

W

W

Y

W

P

P

S

D

A

-

R

R

G

A

R

L

M

A

N

T

A

G

L

F

F

A

M

V

T

V

G

G

I
x
L

P

G

I

L

A

G

G

S

V

F

L

L

G

M

G

G

P

P

L

L

S

A

G

T

W

Y

I

I

M

I

H

E

A

-

F

-

G

-

V

-

H

-

G

-

L

-

A

-

N

-

W

W

Q

R

W

Y

L

V

F

F

L

W

L

Y

-

C

-

G

E

V

A

A

L

M

P

G

S

I

L

M

A

A

L

L

G

-

I

I

A

A

T

G

P

A

F

F

V

L

L

E

P

P

N

P

G

-

I

-

R

-

S

P

A

A

S

G

W

W

L

K

D

P

A

A

R

-

E

-

K

-

Q

-

L

-

E

-

D

-

R

-

L

-

A

G

G

A

D

A

P

P

Q

K

A

V

G

T

S

R

E

D

A

W

S

T

L

Y

R

E

S

E

V

A

M

K

A

G

D

D

V

T

R

K

V

F

W

W

R

L

L

L

A

Y

A

L

I

A

Y

Y

F

F

C

C

C

G

M

S

M

F

G

A

L

G

Y

L

G

M

V

V

S

I

F

G

Y

H

L

L

P

A

T

G

L

F

I

G

A

R

A

D

A

A

G

G

V

L

D

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-

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R

I

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A

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I

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C

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G

G

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W

W

M

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-

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-

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L

A

L

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G

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F

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M

A

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K

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A

binding 2-sulfanylbenzoic acid: Y110 (= Y134), L136 (≠ I155), R256 (≠ M298), Y307 (≠ A348), F311 (≠ S352), C338 (≠ G381)

Query Sequence

>N515DRAFT_2085 FitnessBrowser__Dyella79:N515DRAFT_2085
MPTAPHPVTELETATYAKVTLRLLPFLFICYVAAYLDRVNIGFAKLQMLSDLHFSEAVYG
LGAGVFFIGYFLFEVPSNMLLHRLGARLWISRIMVSWALVSAATMLVTTPTAFYVLRFLL
GVAEAGFFPGIVLYLTYWYPSARRGRMNALFMTGIPIAGVLGGPLSGWIMHAFGGVHGLA
NWQWLFLLEALPSLALGIATPFVLPNGIRSASWLDAREKQLLEDRLAGDPQAGSEASLRS
VMADVRVWRLAAIYFCCMMGLYGVSFYLPTLIAAAGVDDALDVGLLTAIPYAVAVVSMIL
LARSSDRHNERRWHLAAASIAGAAGLYASTLCGSELVLGLIALSLGTAGVLSTMPVFWTW
PSSVLAGTAAAAGIAMINSIGNLAGFVSPSIIGWMKDLTHSTNAGLWVVAVALLLGAALA
LLGSAAKAPARMRA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory