SitesBLAST
Comparing N515DRAFT_3101 FitnessBrowser__Dyella79:N515DRAFT_3101 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4xckA Vibrio cholerae o395 ribokinase complexed with adp, ribose and cesium ion. (see paper)
44% identity, 99% coverage: 1:294/297 of query aligns to 1:303/306 of 4xckA
- active site: A249 (≠ G242), A250 (= A243), G251 (= G244), D252 (= D245)
- binding adenosine-5'-diphosphate: T220 (= T213), G222 (= G215), S223 (≠ G216), V242 (= V235), T247 (= T240), A250 (= A243), F254 (= F247), H276 (≠ C267), A279 (= A270), V283 (= V274)
- binding alpha-D-ribofuranose: N11 (= N11), D13 (= D13), G39 (= G39), K40 (= K40), N43 (= N43), A95 (= A95), I107 (= I106), I109 (≠ V108), E140 (= E139), T248 (= T241), D252 (= D245)
1gqtB Activation of ribokinase by monovalent cations (see paper)
46% identity, 98% coverage: 4:293/297 of query aligns to 6:304/307 of 1gqtB
- active site: A251 (≠ G242), A252 (= A243), G253 (= G244), D254 (= D245)
- binding phosphomethylphosphonic acid adenylate ester: N186 (= N183), T222 (= T213), G224 (= G215), S225 (≠ G216), A252 (= A243), G253 (= G244), H278 (≠ C267), A281 (= A270)
- binding cesium ion: D248 (= D239), I250 (≠ T241), A284 (≠ S273), R287 (= R276), S293 (≠ G282)
- binding alpha-D-ribofuranose: N13 (= N11), D15 (= D13), G41 (= G39), N45 (= N43), E142 (= E139), D254 (= D245)
P0A9J6 Ribokinase; RK; EC 2.7.1.15 from Escherichia coli (strain K12) (see 3 papers)
46% identity, 98% coverage: 4:293/297 of query aligns to 7:305/309 of P0A9J6
1rk2A E. Coli ribokinase complexed with ribose and adp, solved in space group p212121 (see paper)
46% identity, 98% coverage: 4:293/297 of query aligns to 4:302/305 of 1rk2A
- active site: A249 (≠ G242), A250 (= A243), G251 (= G244), D252 (= D245)
- binding adenosine-5'-diphosphate: T220 (= T213), G222 (= G215), S223 (≠ G216), A250 (= A243), G251 (= G244), H276 (≠ C267), A279 (= A270)
- binding tetrafluoroaluminate ion: G213 (≠ A206), R215 (≠ G208)
- binding magnesium ion: D246 (= D239), A282 (≠ S273), R285 (= R276), S291 (≠ G282)
- binding alpha-D-ribofuranose: N11 (= N11), D13 (= D13), G38 (= G38), G39 (= G39), K40 (= K40), N43 (= N43), E140 (= E139), D252 (= D245)
8cqxA Ribokinase from t.Sp mutant a92g
46% identity, 98% coverage: 4:295/297 of query aligns to 2:300/300 of 8cqxA
- binding adenosine-5'-diphosphate: N179 (= N183), T217 (= T213), G219 (= G215), A220 (≠ G216), G222 (= G218), F250 (= F247), N272 (≠ C267), G275 (≠ A270), A276 (= A271), T279 (≠ V274)
- binding magnesium ion: D242 (= D239), T244 (= T241), A278 (≠ S273), S287 (≠ G282)
6znxC Ribokinase from thermus species
42% identity, 98% coverage: 4:295/297 of query aligns to 2:265/265 of 6znxC
5c3yA Structure of human ribokinase crystallized with amppnp
38% identity, 97% coverage: 1:289/297 of query aligns to 1:303/306 of 5c3yA
- active site: G252 (= G242), A253 (= A243), G254 (= G244), D255 (= D245)
- binding amp phosphoramidate: T221 (= T213), G223 (= G215), V245 (= V235), T250 (= T240), G254 (= G244), N281 (≠ C267), A284 (= A270), A285 (= A271)
6wk0B Crystal structure of human ribokinase in complex with amppcp and ribose
38% identity, 97% coverage: 1:289/297 of query aligns to 2:304/311 of 6wk0B
- binding phosphomethylphosphonic acid adenylate ester: N186 (= N183), T222 (= T213), G224 (= G215), A225 (≠ G216), G227 (= G218), T243 (≠ G232), V246 (= V235), A254 (= A243), G255 (= G244), N282 (≠ C267), A285 (= A270), A286 (= A271), V289 (= V274)
- binding alpha-D-ribofuranose: D14 (= D13), G40 (= G39), K41 (= K40), N44 (= N43), A96 (= A95), E141 (= E139), D256 (= D245)
5c41A Crystal structure of human ribokinase in complex with amppcp in p21 spacegroup and with 4 protomers
38% identity, 97% coverage: 1:289/297 of query aligns to 2:304/317 of 5c41A
- active site: G253 (= G242), A254 (= A243), G255 (= G244), D256 (= D245)
- binding phosphomethylphosphonic acid adenylate ester: N186 (= N183), T222 (= T213), G224 (= G215), A225 (≠ G216), G227 (= G218), V246 (= V235), G255 (= G244), N282 (≠ C267), A285 (= A270), A286 (= A271)
2fv7A Crystal structure of human ribokinase
38% identity, 97% coverage: 1:289/297 of query aligns to 1:303/308 of 2fv7A
- active site: G252 (= G242), A253 (= A243), G254 (= G244), D255 (= D245)
- binding adenosine-5'-diphosphate: N185 (= N183), T221 (= T213), G223 (= G215), G226 (= G218), T242 (≠ G232), V245 (= V235), A253 (= A243), G254 (= G244), N281 (≠ C267), A284 (= A270), A285 (= A271), V288 (= V274)
6wjzA Crystal structure of human ribokinase in complex with ampcp
38% identity, 97% coverage: 1:289/297 of query aligns to 2:304/315 of 6wjzA
- binding phosphomethylphosphonic acid adenosyl ester: N186 (= N183), T222 (= T213), G224 (= G215), A225 (≠ G216), G227 (= G218), T243 (≠ G232), V246 (= V235), A254 (= A243), G255 (= G244), N282 (≠ C267), A285 (= A270), A286 (= A271), V289 (= V274)
5byfA Crystal structure of human ribokinase in complex with amp
37% identity, 95% coverage: 8:289/297 of query aligns to 10:305/313 of 5byfA
Q9H477 Ribokinase; RK; EC 2.7.1.15 from Homo sapiens (Human)
37% identity, 95% coverage: 8:289/297 of query aligns to 22:317/322 of Q9H477
6a8cA Ribokinase from leishmania donovani with adp (see paper)
36% identity, 97% coverage: 4:291/297 of query aligns to 16:324/327 of 6a8cA
- binding adenosine-5'-diphosphate: G245 (= G215), A246 (≠ G216), T271 (= T240), A274 (= A243), G275 (= G244), N300 (≠ C267), A303 (= A270)
- binding glycerol: D25 (= D13), S42 (≠ G30), S44 (≠ R32), G50 (= G38), G51 (= G39), N55 (= N43)
6a8bA Ribokinase from leishmania donovani with amppcp (see paper)
36% identity, 97% coverage: 4:291/297 of query aligns to 16:324/327 of 6a8bA
- binding phosphomethylphosphonic acid adenylate ester: G245 (= G215), A246 (≠ G216), T271 (= T240), A274 (= A243), G275 (= G244), N300 (≠ C267), A303 (= A270), V307 (= V274)
- binding glycerol: D25 (= D13), G50 (= G38), G51 (= G39), N55 (= N43), N157 (≠ M138), I159 (= I140), E190 (= E172)
6a8aA Ribokinase from leishmania donovani with atp (see paper)
36% identity, 97% coverage: 4:291/297 of query aligns to 16:324/327 of 6a8aA
- binding adenosine-5'-triphosphate: N207 (= N183), T243 (= T213), G245 (= G215), A246 (≠ G216), G248 (= G218), T271 (= T240), G273 (= G242), A274 (= A243), G275 (= G244), N300 (≠ C267), A303 (= A270), V307 (= V274)
- binding glycerol: D25 (= D13), G50 (= G38), G51 (= G39), N55 (= N43)
6ilsB Structure of arabidopsis thaliana ribokinase complexed with ribose and atp (see paper)
35% identity, 95% coverage: 4:286/297 of query aligns to 5:302/313 of 6ilsB
- binding adenosine-5'-triphosphate: N189 (= N183), K225 (≠ T213), G227 (= G215), I246 (≠ F233), A248 (= A234), A257 (= A243), G258 (= G244), F261 (= F247), A286 (= A270), S287 (≠ A271)
- binding alpha-D-ribofuranose: N12 (= N11), D14 (= D13), G40 (= G39), K41 (= K40), N44 (= N43), E144 (= E139), D259 (= D245)
A1A6H3 Ribokinase; AtRBSK; RK; EC 2.7.1.15 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
35% identity, 95% coverage: 4:286/297 of query aligns to 71:368/379 of A1A6H3
Sites not aligning to the query:
- 1:74 mutation Missing: Loss of ribokinase activity.
3go6B Crystal structure of m. Tuberculosis ribokinase (rv2436) in complex with ribose and amp-pnp
39% identity, 91% coverage: 3:273/297 of query aligns to 3:264/287 of 3go6B
- active site: G227 (= G242), A228 (= A243), G229 (= G244), D230 (= D245)
- binding adenosine-5'-diphosphate: G200 (= G215), V201 (≠ G216), G203 (= G218), V220 (= V235), T225 (= T240), A228 (= A243), G229 (= G244), C258 (= C267), G261 (≠ A270), A262 (= A271)
- binding magnesium ion: D224 (= D239), A226 (≠ T241), A264 (≠ S273)
Sites not aligning to the query:
3go6A Crystal structure of m. Tuberculosis ribokinase (rv2436) in complex with ribose and amp-pnp
39% identity, 91% coverage: 3:273/297 of query aligns to 3:264/287 of 3go6A
- active site: G227 (= G242), A228 (= A243), G229 (= G244), D230 (= D245)
- binding magnesium ion: D224 (= D239), A264 (≠ S273)
- binding alpha-D-ribofuranose: N11 (= N11), D13 (= D13), G39 (= G39), K40 (= K40), N43 (= N43), A94 (= A95), I96 (= I97), V106 (≠ I106), E134 (= E139), D230 (= D245)
Sites not aligning to the query:
Query Sequence
>N515DRAFT_3101 FitnessBrowser__Dyella79:N515DRAFT_3101
MARVVVVGSINMDLVTLAPRFAGPGETVLGERFLTVPGGKGANQAVAAARLGAEVTLIGA
LGDDTFGEQLREGLAREGIALDYVSRIDDCGSGTASITVAGGENEIIVVPAANARVTPAQ
VEAATDAIARADAVLVQMEIPLESVEATLRLGHRLGVPVILNPAPAQKLPTEWLKLARYV
TPNQHELAILLGADAQQDFRALMRQAPGPVVLTRGGEGAWYREDGEPTHQSGFAVDVVDT
TGAGDTFNGALAVFLHEGLPQAVRKACAAAALSVTRLGAQGGMPTRVELDAWLAGRP
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory