SitesBLAST
Comparing N515DRAFT_3206 FitnessBrowser__Dyella79:N515DRAFT_3206 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
7nbgDDD structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
30% identity, 90% coverage: 24:306/316 of query aligns to 23:309/310 of 7nbgDDD
- active site: K53 (= K54), S76 (≠ A77), E202 (≠ V202), A206 (= A206), D208 (≠ S208), G231 (= G230), L304 (= L301), S305 (≠ C302)
- binding calcium ion: E202 (≠ V202), A206 (= A206), D208 (≠ S208)
- binding magnesium ion: N239 (≠ S239)
- binding ortho-xylene: S76 (≠ A77), Q81 (= Q82), I96 (≠ V97), Y113 (≠ L114)
- binding pyridoxal-5'-phosphate: F52 (= F53), K53 (= K54), N78 (= N79), G177 (= G177), G178 (≠ N178), G179 (= G179), G180 (≠ A180), M181 (≠ L181), G231 (= G230), V232 (≠ I231), E275 (= E275), T277 (≠ A277), S305 (≠ C302), G306 (= G303)
Sites not aligning to the query:
3l6bA X-ray crystal structure of human serine racemase in complex with malonate a potent inhibitor (see paper)
30% identity, 90% coverage: 24:306/316 of query aligns to 24:310/322 of 3l6bA
- active site: K54 (= K54), S77 (≠ A77), E203 (≠ V202), A207 (= A206), D209 (≠ S208), G232 (= G230), T278 (≠ A277), L305 (= L301), S306 (≠ C302)
- binding malonate ion: K54 (= K54), S76 (= S76), S77 (≠ A77), N79 (= N79), H80 (≠ F80), R128 (≠ A128), G232 (= G230)
- binding manganese (ii) ion: E203 (≠ V202), A207 (= A206), D209 (≠ S208)
- binding pyridoxal-5'-phosphate: F53 (= F53), K54 (= K54), N79 (= N79), G178 (= G177), G179 (≠ N178), G180 (= G179), G181 (≠ A180), M182 (≠ L181), V233 (≠ I231), E276 (= E275), T278 (≠ A277), S306 (≠ C302), G307 (= G303)
6zspAAA serine racemase bound to atp and malonate. (see paper)
30% identity, 90% coverage: 24:306/316 of query aligns to 23:307/320 of 6zspAAA
- active site: K53 (= K54), S74 (≠ A77), E200 (≠ V202), A204 (= A206), D206 (≠ S208), G229 (= G230), L302 (= L301), S303 (≠ C302)
- binding adenosine-5'-triphosphate: S28 (≠ A29), S29 (≠ A30), I30 (≠ A31), K48 (≠ P49), T49 (≠ I50), Q79 (= Q82), Y111 (≠ L114), E266 (≠ R268), R267 (≠ H269), K269 (≠ G271), N306 (= N305)
- binding magnesium ion: E200 (≠ V202), A204 (= A206), D206 (≠ S208)
- binding malonate ion: K53 (= K54), S73 (= S76), S74 (≠ A77), N76 (= N79), H77 (≠ F80), R125 (≠ A128), G229 (= G230), S232 (≠ V233)
7nbfAAA structure of human serine racemase in complex with DSiP fragment Z126932614, XChem fragment screen (see paper)
30% identity, 90% coverage: 24:306/316 of query aligns to 23:314/323 of 7nbfAAA
- active site: K53 (= K54), S81 (≠ A77), E207 (≠ V202), A211 (= A206), D213 (≠ S208), G236 (= G230), L309 (= L301), S310 (≠ C302)
- binding calcium ion: E207 (≠ V202), A211 (= A206), D213 (≠ S208)
- binding magnesium ion: N244 (≠ S239)
- binding pyridoxal-5'-phosphate: F52 (= F53), K53 (= K54), N83 (= N79), G182 (= G177), G183 (≠ N178), G184 (= G179), G185 (≠ A180), M186 (≠ L181), G236 (= G230), V237 (≠ I231), T282 (≠ A277), S310 (≠ C302), G311 (= G303)
- binding 2-[(methylsulfonyl)methyl]-1H-benzimidazole: T23 (= T24), P24 (= P25), L26 (≠ R27), T27 (≠ R28), F46 (≠ D47)
Sites not aligning to the query:
7nbdAAA structure of human serine racemase in complex with DSiP fragment Z235449082, XChem fragment screen (see paper)
30% identity, 90% coverage: 24:306/316 of query aligns to 23:314/323 of 7nbdAAA
- active site: K53 (= K54), S81 (≠ A77), E207 (≠ V202), A211 (= A206), D213 (≠ S208), G236 (= G230), L309 (= L301), S310 (≠ C302)
- binding calcium ion: E207 (≠ V202), A211 (= A206), D213 (≠ S208)
- binding [4-(1H-benzimidazol-1-yl)phenyl]methanol: W272 (≠ Q267), L278 (≠ V273), V314 (≠ I306)
- binding magnesium ion: N244 (≠ S239)
- binding pyridoxal-5'-phosphate: F52 (= F53), K53 (= K54), N83 (= N79), G182 (= G177), G183 (≠ N178), G184 (= G179), G185 (≠ A180), M186 (≠ L181), G236 (= G230), V237 (≠ I231), E280 (= E275), T282 (≠ A277), S310 (≠ C302), G311 (= G303)
Sites not aligning to the query:
7nbcCCC structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
30% identity, 90% coverage: 24:306/316 of query aligns to 23:314/323 of 7nbcCCC
- active site: K53 (= K54), S81 (≠ A77), E207 (≠ V202), A211 (= A206), D213 (≠ S208), G236 (= G230), L309 (= L301), S310 (≠ C302)
- binding biphenyl-4-ylacetic acid: T78 (≠ S74), H79 (≠ A75), H84 (≠ F80), V148 (= V144), H149 (≠ E145), P150 (≠ D146)
- binding calcium ion: E207 (≠ V202), A211 (= A206), D213 (≠ S208)
- binding pyridoxal-5'-phosphate: F52 (= F53), K53 (= K54), N83 (= N79), G182 (= G177), G183 (≠ N178), G184 (= G179), G185 (≠ A180), M186 (≠ L181), G236 (= G230), V237 (≠ I231), T282 (≠ A277), S310 (≠ C302), G311 (= G303)
7nbcAAA structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
30% identity, 90% coverage: 24:306/316 of query aligns to 23:314/323 of 7nbcAAA
- active site: K53 (= K54), S81 (≠ A77), E207 (≠ V202), A211 (= A206), D213 (≠ S208), G236 (= G230), L309 (= L301), S310 (≠ C302)
- binding calcium ion: E207 (≠ V202), A211 (= A206), D213 (≠ S208)
- binding magnesium ion: N244 (≠ S239)
- binding pyridoxal-5'-phosphate: F52 (= F53), K53 (= K54), N83 (= N79), G182 (= G177), G183 (≠ N178), G184 (= G179), G185 (≠ A180), M186 (≠ L181), G236 (= G230), V237 (≠ I231), T282 (≠ A277), S310 (≠ C302), G311 (= G303)
Sites not aligning to the query:
P04968 L-threonine dehydratase biosynthetic IlvA; Threonine deaminase; EC 4.3.1.19 from Escherichia coli (strain K12) (see paper)
31% identity, 90% coverage: 24:307/316 of query aligns to 32:320/514 of P04968
- K62 (= K54) modified: N6-(pyridoxal phosphate)lysine
- N89 (= N79) binding
- GGGGL 188:192 (≠ GNGAL 177:181) binding
- S315 (≠ C302) binding
7nbgAAA structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
30% identity, 90% coverage: 24:306/316 of query aligns to 23:314/322 of 7nbgAAA
- active site: K53 (= K54), S81 (≠ A77), E207 (≠ V202), A211 (= A206), D213 (≠ S208), G236 (= G230), L309 (= L301), S310 (≠ C302)
- binding calcium ion: E207 (≠ V202), A211 (= A206), D213 (≠ S208)
- binding pyridoxal-5'-phosphate: F52 (= F53), K53 (= K54), N83 (= N79), G182 (= G177), G183 (≠ N178), G184 (= G179), G185 (≠ A180), M186 (≠ L181), G236 (= G230), V237 (≠ I231), T282 (≠ A277), S310 (≠ C302), G311 (= G303)
- binding ~{N}-[2-(2-methylphenyl)ethyl]ethanamide: S81 (≠ A77), G85 (= G81), Q86 (= Q82), I101 (≠ V97), K111 (= K107), I115 (≠ M111), Y118 (≠ L114)
7nbhAAA structure of human serine racemase in complex with DSiP fragment Z26781964, XChem fragment screen (see paper)
30% identity, 90% coverage: 24:306/316 of query aligns to 23:314/320 of 7nbhAAA
- active site: K53 (= K54), S81 (≠ A77), E207 (≠ V202), A211 (= A206), D213 (≠ S208), G236 (= G230), L309 (= L301), S310 (≠ C302)
- binding calcium ion: E207 (≠ V202), A211 (= A206), D213 (≠ S208)
- binding N-[(1H-benzimidazol-2-yl)methyl]furan-2-carboxamide: S81 (≠ A77), G85 (= G81), Q86 (= Q82), K111 (= K107), I115 (≠ M111), Y118 (≠ L114), D235 (= D229), P281 (= P276), N313 (= N305), V314 (≠ I306)
Sites not aligning to the query:
1tdjA Threonine deaminase (biosynthetic) from e. Coli (see paper)
31% identity, 90% coverage: 24:307/316 of query aligns to 28:316/494 of 1tdjA
- active site: K58 (= K54), A83 (= A77), E209 (≠ V202), S213 (≠ A206), C215 (≠ S208), G237 (= G230), L310 (= L301), S311 (≠ C302)
- binding pyridoxal-5'-phosphate: F57 (= F53), K58 (= K54), N85 (= N79), G184 (= G177), G185 (≠ N178), G186 (= G179), G187 (≠ A180), G237 (= G230), E282 (= E275), S311 (≠ C302), G312 (= G303)
Q7XSN8 Serine racemase; D-serine dehydratase; D-serine ammonia-lyase; L-serine dehydratase; L-serine ammonia-lyase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Oryza sativa subsp. japonica (Rice) (see paper)
29% identity, 95% coverage: 6:306/316 of query aligns to 20:327/339 of Q7XSN8
- E219 (≠ V202) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-225.
- D225 (≠ S208) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-219.
5cvcA Structure of maize serine racemase (see paper)
27% identity, 95% coverage: 6:306/316 of query aligns to 4:311/329 of 5cvcA
- active site: K52 (= K54), S77 (≠ A77), E203 (≠ V202), A207 (= A206), D209 (≠ S208), G231 (= G230), V306 (≠ L301), S307 (≠ C302)
- binding magnesium ion: E203 (≠ V202), A207 (= A206), D209 (≠ S208)
- binding pyridoxal-5'-phosphate: F51 (= F53), K52 (= K54), N79 (= N79), S178 (≠ G177), G179 (≠ N178), G180 (= G179), G181 (≠ A180), L232 (≠ I231), E275 (= E275), S307 (≠ C302), G308 (= G303)
1ve5A Crystal structure of t.Th. Hb8 threonine deaminase
36% identity, 95% coverage: 6:305/316 of query aligns to 2:303/308 of 1ve5A
- active site: K50 (= K54), S56 (≠ F61), S72 (≠ A77), E200 (≠ V202), A204 (= A206), D206 (≠ S208), G229 (= G230), L299 (= L301), S300 (≠ C302)
- binding calcium ion: E200 (≠ V202), A204 (= A206), D206 (≠ S208)
- binding pyridoxal-5'-phosphate: F49 (= F53), K50 (= K54), N74 (= N79), G175 (= G177), G176 (≠ N178), G177 (= G179), G178 (≠ A180), E274 (= E275), T276 (≠ A277), S300 (≠ C302), G301 (= G303)
A4F2N8 L-threo-3-hydroxyaspartate ammonia-lyase; L-threo-3-hydroxyaspartate dehydratase; L-THA DH; EC 4.3.1.16 from Pseudomonas sp. (see paper)
28% identity, 97% coverage: 1:306/316 of query aligns to 1:308/319 of A4F2N8
- K53 (= K54) mutation to A: Loss of enzymatic activity.
3hmkA Crystal structure of serine racemase (see paper)
27% identity, 91% coverage: 18:306/316 of query aligns to 19:315/321 of 3hmkA
- active site: K54 (= K54), S82 (≠ A77), E208 (≠ V202), A212 (= A206), D214 (≠ S208), G237 (= G230), T283 (≠ A277), L310 (= L301), S311 (≠ C302)
- binding manganese (ii) ion: E208 (≠ V202), A212 (= A206), D214 (≠ S208)
- binding pyridoxal-5'-phosphate: F53 (= F53), K54 (= K54), N84 (= N79), G183 (vs. gap), G184 (vs. gap), G185 (vs. gap), G186 (≠ A180), M187 (≠ L181), G237 (= G230), V238 (≠ I231), T283 (≠ A277), S311 (≠ C302), G312 (= G303)
Q9QZX7 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Mus musculus (Mouse) (see paper)
27% identity, 90% coverage: 24:306/316 of query aligns to 26:317/339 of Q9QZX7
- C113 (≠ A106) modified: S-nitrosocysteine; mutation to S: Abolishes S-nitrosylation.
O59791 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 4.3.1.17; EC 4.3.1.18; EC 5.1.1.18 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
25% identity, 95% coverage: 7:306/316 of query aligns to 10:312/323 of O59791
- S82 (≠ A77) mutation to A: Loss of racemase activity. Reduces D-serine dehydratase activity by 99%. Slightly reduced L-serine dehydratase activity.
2zr8A Crystal structure of modified serine racemase complexed with serine (see paper)
25% identity, 95% coverage: 7:306/316 of query aligns to 6:308/319 of 2zr8A
- active site: K53 (= K54), S78 (≠ A77), E204 (≠ V202), G208 (≠ A206), D210 (≠ S208), G232 (= G230), I303 (≠ L301), S304 (≠ C302)
- binding magnesium ion: E204 (≠ V202), G208 (≠ A206), D210 (≠ S208)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F53), K53 (= K54), S77 (= S76), S78 (≠ A77), N80 (= N79), H81 (≠ F80), P147 (≠ D146), G179 (= G177), G180 (≠ N178), G181 (= G179), G182 (≠ A180), G232 (= G230), E277 (= E275), T279 (≠ A277), S304 (≠ C302)
- binding serine: S78 (≠ A77), R129 (≠ A128), D231 (= D229), G232 (= G230), A233 (≠ I231), Q234 (≠ A232), T235 (≠ V233)
2zpuA Crystal structure of modified serine racemase from s.Pombe. (see paper)
25% identity, 95% coverage: 7:306/316 of query aligns to 6:308/319 of 2zpuA
- active site: K53 (= K54), S78 (≠ A77), E204 (≠ V202), G208 (≠ A206), D210 (≠ S208), G232 (= G230), I303 (≠ L301), S304 (≠ C302)
- binding magnesium ion: E204 (≠ V202), G208 (≠ A206), D210 (≠ S208)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F53), K53 (= K54), S77 (= S76), S78 (≠ A77), N80 (= N79), H81 (≠ F80), P147 (≠ D146), G179 (= G177), G180 (≠ N178), G181 (= G179), G182 (≠ A180), G232 (= G230), E277 (= E275), T279 (≠ A277), S304 (≠ C302)
Query Sequence
>N515DRAFT_3206 FitnessBrowser__Dyella79:N515DRAFT_3206
MTLSLDLDGVLAASPQIDPVFLHTPLRRAAALDDALGCRLLGKDETDNPIGSFKGRGTEW
FVATALNSGEAIVSASAGNFGQGLARAATRRGHACTVFAAQTANPAKLEAMRALGAEVRL
AGVDFDAAKSAARQYAEEHRLRFVEDGAEPAIAQGAATIALELLAEAAPEVVLVPLGNGA
LLEGIGSVLRARAPSVEIVAVVAAGAPSMKLSLEAGRAIETAHADTIADGIAVRVPIASR
LPQLRRCCDRIVAVSEAQLLDAMRLIQRHLGMVVEPAGAAGVAGILAEPARHAGRLVATV
LCGKNIHPSLRDRLIP
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory