SitesBLAST
Comparing N515DRAFT_3582 N515DRAFT_3582 FAD/FMN-containing dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
39% identity, 94% coverage: 26:466/467 of query aligns to 20:464/465 of 3pm9A
- active site: A149 (= A155), L159 (≠ N165)
- binding flavin-adenine dinucleotide: P69 (= P75), Q70 (≠ S76), G71 (= G77), G72 (= G78), N73 (≠ R79), T74 (= T80), G75 (= G81), L76 (= L82), G79 (= G85), Q80 (≠ A86), L91 (= L97), L133 (≠ F139), G134 (≠ A140), A135 (= A141), C139 (= C145), T140 (≠ S146), G142 (= G148), G143 (= G149), S146 (≠ A152), T147 (= T153), A149 (= A155), G150 (= G156), E200 (= E206), G201 (= G207), I205 (= I211), I206 (≠ V212), E423 (= E425)
6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
37% identity, 94% coverage: 25:465/467 of query aligns to 25:462/466 of 6lpxA
- binding 2-oxoglutaric acid: R333 (= R330), T337 (= T334), K348 (= K344), Y379 (≠ F377), H381 (= H379), H388 (= H386), H423 (= H426)
- binding flavin-adenine dinucleotide: W39 (= W39), P75 (= P75), Q76 (≠ S76), G77 (= G77), G78 (= G78), N79 (≠ R79), T80 (= T80), G81 (= G81), M82 (≠ L82), G85 (= G85), S86 (≠ A86), L139 (≠ F139), G140 (≠ A140), A141 (= A141), C145 (= C145), G149 (= G149), N150 (= N150), A152 (= A152), T153 (= T153), G157 (= G157), G207 (= G207), I212 (≠ V212), E422 (= E425), N459 (= N462)
- binding zinc ion: H381 (= H379), H388 (= H386), E422 (= E425)
6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
37% identity, 94% coverage: 25:465/467 of query aligns to 25:462/466 of 6lpuA
- binding flavin-adenine dinucleotide: W39 (= W39), P75 (= P75), G77 (= G77), G78 (= G78), N79 (≠ R79), T80 (= T80), G81 (= G81), G85 (= G85), S86 (≠ A86), L139 (≠ F139), G140 (≠ A140), A141 (= A141), C145 (= C145), H146 (≠ S146), G148 (= G148), G149 (= G149), N150 (= N150), A152 (= A152), T153 (= T153), A155 (= A155), E206 (= E206), G207 (= G207), I211 (= I211), I212 (≠ V212), E422 (= E425), N459 (= N462)
- binding (2s)-2-hydroxypentanedioic acid: R333 (= R330), T337 (= T334), K348 (= K344), Y379 (≠ F377), H381 (= H379), H388 (= H386), H423 (= H426)
- binding zinc ion: H381 (= H379), H388 (= H386), E422 (= E425)
6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
37% identity, 94% coverage: 25:465/467 of query aligns to 25:462/466 of 6lpqA
- binding flavin-adenine dinucleotide: W39 (= W39), P75 (= P75), G77 (= G77), G78 (= G78), N79 (≠ R79), T80 (= T80), G81 (= G81), G85 (= G85), S86 (≠ A86), L139 (≠ F139), G140 (≠ A140), A141 (= A141), C145 (= C145), H146 (≠ S146), G149 (= G149), N150 (= N150), A152 (= A152), T153 (= T153), A155 (= A155), G157 (= G157), E206 (= E206), G207 (= G207), I211 (= I211), I212 (≠ V212), E422 (= E425), N459 (= N462)
- binding d-malate: M82 (≠ L82), R333 (= R330), T337 (= T334), K348 (= K344), Y379 (≠ F377), H381 (= H379), H388 (= H386), E422 (= E425), H423 (= H426)
- binding zinc ion: H381 (= H379), H388 (= H386), E422 (= E425)
6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
37% identity, 94% coverage: 25:465/467 of query aligns to 25:462/466 of 6lppA
- binding (2R)-2-hydroxypentanedioic acid: R333 (= R330), T337 (= T334), K348 (= K344), Y379 (≠ F377), H381 (= H379), H388 (= H386), N390 (= N388), H423 (= H426)
- binding flavin-adenine dinucleotide: W39 (= W39), P75 (= P75), G77 (= G77), G78 (= G78), N79 (≠ R79), T80 (= T80), G81 (= G81), M82 (≠ L82), G85 (= G85), S86 (≠ A86), L139 (≠ F139), G140 (≠ A140), A141 (= A141), C145 (= C145), G149 (= G149), N150 (= N150), A152 (= A152), T153 (= T153), A155 (= A155), G157 (= G157), G207 (= G207), I212 (≠ V212), E422 (= E425), H423 (= H426)
- binding zinc ion: H381 (= H379), H388 (= H386), E422 (= E425)
6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
37% identity, 94% coverage: 25:465/467 of query aligns to 26:463/467 of 6lpnB
- binding flavin-adenine dinucleotide: W40 (= W39), P76 (= P75), G78 (= G77), G79 (= G78), N80 (≠ R79), T81 (= T80), G82 (= G81), M83 (≠ L82), G86 (= G85), S87 (≠ A86), L140 (≠ F139), A142 (= A141), C146 (= C145), H147 (≠ S146), G150 (= G149), N151 (= N150), A153 (= A152), T154 (= T153), G208 (= G207), I212 (= I211), I213 (≠ V212), E423 (= E425), N460 (= N462)
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
37% identity, 94% coverage: 25:465/467 of query aligns to 78:515/521 of Q8N465
- S109 (≠ V56) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ V74) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (= G78) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (≠ V94) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (= M100) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- C172 (≠ I119) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (= P136) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (= A152) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (≠ G178) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (= G180) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
- D375 (≠ S319) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- R386 (= R330) binding ; binding ; binding ; mutation to A: Loss of catalytic activity.
- T390 (= T334) binding ; binding ; mutation to A: Significantly reduced catalytic activity.
- V399 (≠ P342) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- K401 (= K344) binding ; binding ; mutation to A: Loss of catalytic activity.
- R419 (≠ Q362) to H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- A426 (≠ Y369) to T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- H434 (= H379) binding ; mutation to A: Loss of catalytic activity.
- G436 (= G381) to V: slight reduction in catalytic activity
- N439 (= N384) to D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- H441 (= H386) binding ; mutation to A: Loss of catalytic activity.
- N443 (= N388) binding ; mutation to A: Significantly reduced catalytic activity.
- V444 (= V389) to A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- A446 (≠ C404) to V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- E475 (= E425) binding ; mutation to A: Loss of catalytic activity.
- H476 (= H426) binding ; binding ; binding ; mutation to A: Loss of catalytic activity.
- G477 (= G427) to R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
P39976 D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3; D-2HG--pyruvate transhydrogenase DLD3; (R)-2-hydroxyglutarate--pyruvate transhydrogenase; D-lactate dehydrogenase [cytochrome] 3; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.99.40; EC 1.1.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
33% identity, 94% coverage: 28:465/467 of query aligns to 49:494/496 of P39976
Sites not aligning to the query:
- 17 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
31% identity, 99% coverage: 6:466/467 of query aligns to 7:463/467 of 7qh2C
- binding flavin-adenine dinucleotide: V73 (≠ P75), G75 (= G77), S76 (≠ G78), G77 (≠ R79), T78 (= T80), G79 (= G81), L80 (= L82), A83 (≠ G85), C84 (≠ A86), P137 (≠ A140), G138 (≠ A141), E139 (≠ R142), A142 (≠ C145), T143 (≠ S146), G146 (= G149), N147 (= N150), S149 (≠ A152), T150 (= T153), A152 (= A155), G153 (= G156), E203 (= E206), G204 (= G207), I209 (≠ V212), E422 (= E425), H423 (= H426)
- binding fe (iii) ion: H377 (= H379), H384 (= H386), E422 (= E425)
P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
32% identity, 97% coverage: 13:467/467 of query aligns to 1:456/459 of P9WIT1
- K354 (≠ A356) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
31% identity, 94% coverage: 27:467/467 of query aligns to 20:455/455 of 8jdxA
- binding flavin-adenine dinucleotide: E32 (≠ W39), P68 (= P75), G70 (= G77), T71 (≠ G78), G72 (≠ R79), T73 (= T80), G74 (= G81), G78 (= G85), V79 (≠ A86), L90 (= L97), P132 (≠ F139), G133 (≠ A140), A134 (= A141), G140 (= G149), M141 (≠ N150), A143 (= A152), T144 (= T153), A146 (= A155), S147 (≠ G156), E200 (= E206), G201 (= G207), I206 (≠ V212), W322 (≠ Q322), E413 (= E425), H414 (= H426), N450 (= N462)
- binding 3-methyl-2-oxobutanoic acid: R318 (≠ Q318), H369 (= H379), H376 (= H386), H414 (= H426)
- binding manganese (ii) ion: H369 (= H379), H376 (= H386), E413 (= E425)
8jdzA Crystal structure of mldhd in complex with 2-keto-3-methylvaleric acid (see paper)
30% identity, 94% coverage: 27:467/467 of query aligns to 20:454/454 of 8jdzA
- binding (3S)-3-methyl-2-oxopentanoic acid: R318 (≠ Q318), W322 (≠ Q322), H369 (= H379), H376 (= H386), H413 (= H426)
- binding flavin-adenine dinucleotide: E32 (≠ W39), P68 (= P75), G70 (= G77), T71 (≠ G78), G72 (≠ R79), T73 (= T80), G74 (= G81), G78 (= G85), V79 (≠ A86), L90 (= L97), P132 (≠ F139), G133 (≠ A140), A134 (= A141), G140 (= G149), M141 (≠ N150), A143 (= A152), T144 (= T153), A146 (= A155), S147 (≠ G156), E200 (= E206), G201 (= G207), I206 (≠ V212), W322 (≠ Q322), E412 (= E425), H413 (= H426), N449 (= N462)
- binding manganese (ii) ion: H369 (= H379), H376 (= H386), E412 (= E425)
8jdyA Crystal structure of mldhd in complex with 2-ketoisocaproic acid (see paper)
30% identity, 94% coverage: 27:467/467 of query aligns to 20:454/454 of 8jdyA
- binding 2-oxo-4-methylpentanoic acid: R318 (≠ Q318), W322 (≠ Q322), S336 (≠ K344), H369 (= H379), H376 (= H386), H413 (= H426)
- binding flavin-adenine dinucleotide: P68 (= P75), G70 (= G77), T71 (≠ G78), G72 (≠ R79), T73 (= T80), G74 (= G81), G78 (= G85), V79 (≠ A86), L90 (= L97), P132 (≠ F139), G133 (≠ A140), A134 (= A141), G140 (= G149), M141 (≠ N150), A143 (= A152), T144 (= T153), A146 (= A155), S147 (≠ G156), E200 (= E206), G201 (= G207), I206 (≠ V212), E412 (= E425), N449 (= N462)
- binding manganese (ii) ion: H369 (= H379), H376 (= H386), E412 (= E425)
8jdpA Crystal structure of h405a mldhd in complex with d-2-hydroxyisovaleric acid (see paper)
29% identity, 94% coverage: 27:467/467 of query aligns to 20:455/455 of 8jdpA
- binding flavin-adenine dinucleotide: P68 (= P75), G70 (= G77), T71 (≠ G78), G72 (≠ R79), T73 (= T80), G74 (= G81), G78 (= G85), V79 (≠ A86), L90 (= L97), P132 (≠ F139), G133 (≠ A140), A134 (= A141), G140 (= G149), M141 (≠ N150), A143 (= A152), T144 (= T153), A146 (= A155), S147 (≠ G156), E200 (= E206), G201 (= G207), I206 (≠ V212), H369 (= H379), E413 (= E425), N450 (= N462)
- binding deaminohydroxyvaline: R319 (= R330), H414 (= H426)
8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
29% identity, 94% coverage: 27:467/467 of query aligns to 20:456/456 of 8jdsA
- binding flavin-adenine dinucleotide: E32 (≠ W39), P68 (= P75), G70 (= G77), T71 (≠ G78), G72 (≠ R79), T73 (= T80), G74 (= G81), G78 (= G85), V79 (≠ A86), L90 (= L97), P132 (≠ F139), G133 (≠ A140), A134 (= A141), G140 (= G149), M141 (≠ N150), A143 (= A152), T144 (= T153), A146 (= A155), S147 (≠ G156), E200 (= E206), G201 (= G207), I206 (≠ V212), W323 (vs. gap), E414 (= E425), H415 (= H426), N451 (= N462)
- binding manganese (ii) ion: H370 (= H379), H377 (= H386), E414 (= E425)
- binding pyruvic acid: R319 (= R330), H370 (= H379), H377 (= H386), H415 (= H426)
8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
30% identity, 94% coverage: 27:467/467 of query aligns to 20:454/454 of 8jdvA
- binding 2-Ketohexanoic acid: V75 (≠ L82), R317 (≠ Q318), W321 (≠ Q322), H368 (= H379), H375 (= H386), H413 (= H426)
- binding flavin-adenine dinucleotide: P68 (= P75), G70 (= G77), T71 (≠ G78), G72 (≠ R79), T73 (= T80), G74 (= G81), G78 (= G85), V79 (≠ A86), L90 (= L97), P132 (≠ F139), G133 (≠ A140), A134 (= A141), G140 (= G149), M141 (≠ N150), A143 (= A152), T144 (= T153), A146 (= A155), S147 (≠ G156), E200 (= E206), G201 (= G207), I206 (≠ V212), W321 (≠ Q322), Y322 (≠ A323), E412 (= E425), H413 (= H426), N449 (= N462)
- binding manganese (ii) ion: H368 (= H379), H375 (= H386), E412 (= E425)
8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
30% identity, 94% coverage: 27:467/467 of query aligns to 20:455/455 of 8jdeA
- binding flavin-adenine dinucleotide: P68 (= P75), G70 (= G77), T71 (≠ G78), G72 (≠ R79), T73 (= T80), G74 (= G81), G78 (= G85), V79 (≠ A86), L90 (= L97), P132 (≠ F139), G133 (≠ A140), A134 (= A141), G140 (= G149), M141 (≠ N150), A143 (= A152), T144 (= T153), A146 (= A155), S147 (≠ G156), E200 (= E206), G201 (= G207), I206 (≠ V212), W322 (≠ Q322), E413 (= E425), H414 (= H426), N450 (= N462)
- binding lactic acid: R318 (≠ Q318), H369 (= H379), H376 (= H386), H414 (= H426)
- binding manganese (ii) ion: H369 (= H379), H376 (= H386), E413 (= E425)
8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
30% identity, 94% coverage: 27:467/467 of query aligns to 20:455/455 of 8jdtA
- binding 2-ketobutyric acid: R318 (≠ Q318), H369 (= H379), H376 (= H386), H414 (= H426)
- binding flavin-adenine dinucleotide: P68 (= P75), G70 (= G77), T71 (≠ G78), G72 (≠ R79), T73 (= T80), G74 (= G81), G78 (= G85), V79 (≠ A86), L90 (= L97), P132 (≠ F139), G133 (≠ A140), A134 (= A141), G140 (= G149), M141 (≠ N150), A143 (= A152), T144 (= T153), A146 (= A155), S147 (≠ G156), E200 (= E206), G201 (= G207), I206 (≠ V212), W322 (≠ Q322), E413 (= E425), H414 (= H426), N450 (= N462)
- binding manganese (ii) ion: H369 (= H379), H376 (= H386), E413 (= E425)
8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
30% identity, 94% coverage: 27:467/467 of query aligns to 20:455/455 of 8jduA
- binding 2-oxopentanoic acid: R318 (= R330), W322 (vs. gap), H369 (= H379), H376 (= H386), H414 (= H426)
- binding flavin-adenine dinucleotide: P68 (= P75), G70 (= G77), T71 (≠ G78), G72 (≠ R79), T73 (= T80), G74 (= G81), G78 (= G85), V79 (≠ A86), L90 (= L97), P132 (≠ F139), G133 (≠ A140), A134 (= A141), G140 (= G149), M141 (≠ N150), A143 (= A152), T144 (= T153), A146 (= A155), S147 (≠ G156), E200 (= E206), G201 (= G207), I206 (≠ V212), W322 (vs. gap), E413 (= E425), N450 (= N462)
- binding manganese (ii) ion: H369 (= H379), H376 (= H386), E413 (= E425)
8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid (see paper)
29% identity, 94% coverage: 27:467/467 of query aligns to 20:456/456 of 8jdrA
- binding flavin-adenine dinucleotide: P68 (= P75), G70 (= G77), T71 (≠ G78), G72 (≠ R79), T73 (= T80), G74 (= G81), G78 (= G85), V79 (≠ A86), L90 (= L97), P132 (≠ F139), G133 (≠ A140), A134 (= A141), G140 (= G149), M141 (≠ N150), A143 (= A152), T144 (= T153), A146 (= A155), S147 (≠ G156), E200 (= E206), G201 (= G207), I206 (≠ V212), Y324 (vs. gap), H370 (= H379), E414 (= E425), N451 (= N462)
- binding (2R,3S)-3-methyl-2-oxidanyl-pentanoic acid: R319 (= R330), W323 (vs. gap), H415 (= H426)
Query Sequence
>N515DRAFT_3582 N515DRAFT_3582 FAD/FMN-containing dehydrogenase
MTAPAQSPKVAALAELAARLPGLRLLTAPADLEHYGRDWTRRWTPAPLAIALPATVEEAQ
AVLRWANEHGVAVVPSGGRTGLSGGAVAAQGELVLSLERMNRVLGFDPIDRTLTVQAGIA
LQAVHDAARSHGLIYPVDFAARGSCSIGGNIATNAGGIRVIRYGNTREWIAGLKVIAGNG
ELLELNRGLIKNSSGYDLRHLTIGSEGTLGIVVEATLRLAEPPPPSQVMLLALPDMAALM
QVFALFRARLKLQAFEFFTDIALRHVLAHGAQRALDGEHPFYVVTEFDAADEAAQDAALA
AFEHGVNEGWIADGAIAQSEAQAAALWRLREGITESLAPHKPYKNDISLRIGALPAFLDE
IQALLGDAYPHFEVVWFGHIGDGNLHINVLKPEGLADAEFIAQCEQVTKLLAAALQRHGG
SISAEHGIGLVKKPYLESTRSAAEIALMRGVKQVFDPNGILNPGKLF
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory