SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing N515DRAFT_4259 FitnessBrowser__Dyella79:N515DRAFT_4259 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

G3XD01 UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronate N-acetyltransferase; UDP-D-GlcNAc3NA N-acetyltransferase; UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronic acid 3-N-acetyltransferase; EC 2.3.1.201 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
78% identity, 99% coverage: 1:193/195 of query aligns to 1:191/191 of G3XD01

query
sites
G3XD01

M

M

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K136 (≠ Q136) mutation to A: Reduction in catalytic activity.; mutation to R: No effect on catalytic activity.

3mqhA Crystal structure of the 3-n-acetyl transferase wlbb from bordetella petrii in complex with coa and udp-3-amino-2-acetamido-2,3-dideoxy glucuronic acid (see paper)
76% identity, 94% coverage: 6:189/195 of query aligns to 6:189/191 of 3mqhA

query
sites
3mqhA

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Q

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binding coenzyme a: F76 (= F76), T84 (= T84), N85 (= N85), V86 (= V86), Y87 (= Y87), P89 (= P89), G111 (= G111), A112 (= A112), A130 (= A130), N135 (≠ T135), V145 (= V145), P148 (= P148), R150 (= R150), Q161 (= Q161)
binding (2S,3S,4R,5R,6R)-5-(acetylamino)-4-amino-6-{[(R)-{[(R)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-3-hydroxytetrahydro-2H-pyran-2-carboxylic acid: W24 (= W24), H28 (= H28), F46 (= F46), Q60 (= Q60), N61 (= N61), Y66 (= Y66), F76 (= F76), N85 (= N85), V86 (= V86), R95 (= R95), Y99 (= Y99)

3mqgC Crystal structure of the 3-n-acetyl transferase wlbb from bordetella petrii in complex with acetyl-coa (see paper)
76% identity, 94% coverage: 6:189/195 of query aligns to 7:190/192 of 3mqgC

query
sites
3mqgC

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binding acetyl coenzyme *a: Y67 (= Y66), F77 (= F76), P80 (= P79), T85 (= T84), N86 (= N85), V87 (= V86), Y88 (= Y87), P90 (= P89), G112 (= G111), A113 (= A112), F128 (= F127), G130 (= G129), A131 (= A130), N136 (≠ T135), V146 (= V145), P149 (= P148), R151 (= R150), Q162 (= Q161)
binding uridine-5'-monophosphate: H29 (= H28), F47 (= F46)
binding uridine-5'-diphosphate: W25 (= W24), S41 (= S40), N62 (= N61)

4mzuF Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
38% identity, 78% coverage: 13:164/195 of query aligns to 9:153/294 of 4mzuF

query
sites
4mzuF

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binding coenzyme a: N81 (= N85), D82 (≠ V86), K83 (≠ Y87), G100 (= G111), A101 (= A112), L106 (≠ V117), G118 (= G129), A119 (= A130), T124 (= T135), K125 (≠ Q136), I134 (≠ V145), N136 (≠ V147), R139 (= R150)
binding thymine: W20 (= W24), Q21 (≠ H25), C38 (≠ G42)

Sites not aligning to the query:

binding thymidine-5'-diphosphate: 189, 203, 205, 270

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
39% identity, 79% coverage: 13:167/195 of query aligns to 9:154/290 of 4mzuB

query
sites
4mzuB

E

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binding coenzyme a: F72 (= F76), T80 (= T84), N81 (= N85), D82 (≠ V86), K83 (≠ Y87), P85 (= P89), G99 (= G111), A100 (= A112), G117 (= G129), A118 (= A130), T123 (= T135), K124 (≠ Q136), I133 (≠ V145), R138 (= R150)
binding magnesium ion: G58 (≠ N62), N76 (≠ S80)
binding thymine: W20 (= W24), Q21 (≠ H25), C38 (≠ G42), A39 (≠ Q43)

Sites not aligning to the query:

binding coenzyme a: 199
binding thymidine-5'-diphosphate: 171, 176, 189, 191, 202, 203, 270

7s45A Crystal structure of an n-acetyltransferase, c80t mutant, from helicobacter pullorum in the presence of acetyl coenzyme a and dtdp (see paper)
41% identity, 71% coverage: 3:141/195 of query aligns to 2:134/141 of 7s45A

query
sites
7s45A

H

H

Y

M

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binding acetyl coenzyme *a: W64 (≠ Y66), T82 (= T84), N83 (= N85), D84 (≠ V86), K85 (≠ Y87), Y86 (≠ N88), P87 (= P89), L110 (≠ V117), T128 (= T135), K129 (≠ Q136)
binding thymidine-5'-diphosphate: F44 (= F46), E46 (≠ G48)

7s42A Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-acetamido-3,6-dideoxy-d- galactose (see paper)
41% identity, 71% coverage: 3:141/195 of query aligns to 2:138/145 of 7s42A

query
sites
7s42A

H

H

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W

H

Q

F

F

V

C

H

V

V

V

C

L

G

P

G

S

A

A

R

I

I

I

G

G

K

E

G

N

V

C

S

N

L

I

G

C

Q

S

N

H

V

C

F
|
F

V

I

G
x
E

N

N

R

D

V

V

S

K

I

I

G

G

D

N

H

N

C

V

K

T

V

I

Q

K

N

C

N

G

V

V

S

Q

V

I

Y
x
W

D

D

N

G

V

I

T

E

L

I

E

E

E

D

G

D

V

V

F

F

C

I

G

G

P

P

S

N

M

V

V

T

F

F

T
x
C

N
|
N

V
x
D

Y
x
K

N
x
Y

P
|
P

R

R

S

S

L

K

I

-

E

Q

R
x
Y

K

P

D

K

E

E

Y

F

R

S

D

K

T

T

L

I

V

I

K

K

R

K

G

G

A

A

T

S

L

I

G

G

A

A

N

N

C

A

T

T

I

I

V
x
L

C

P

G

G

I

I

T

T

I

I

G

G

E

E

Y

N

A

A

F

M

V

I

G

G

A

A

G

G

A

A

V

I

V

V

T
|
T

Q
x
K

D

D

V

V

S

L

P

P

Y

H

binding [(2~{R},3~{R},4~{S},5~{R},6~{R})-4-acetamido-6-methyl-3,5-bis(oxidanyl)oxan-2-yl] [[(2~{R},3~{S},5~{R})-5-[5-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: F44 (= F46), E46 (≠ G48), W64 (≠ Y66), N83 (= N85), D84 (≠ V86), Y92 (≠ R95)
binding coenzyme a: C82 (≠ T84), D84 (≠ V86), K85 (≠ Y87), Y86 (≠ N88), P87 (= P89), L114 (≠ V117), T132 (= T135), K133 (≠ Q136)

7s41A Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-acetamido-3,6-dideoxy-d- glucose (see paper)
41% identity, 71% coverage: 3:141/195 of query aligns to 2:138/145 of 7s41A

query
sites
7s41A

H

H

Y

M

Q

I

H

H

S

K

S

L

A

A

I

D

V

V

D

-

E

Q

G

S

A

K

Q

N

I

I

G

G

E

S

G

G

S

T

R

R

V

I

W

W

H

Q

F

F

V

C

H

V

V

V

C

L

G

P

G

S

A

A

R

I

I

I

G

G

K

E

G

N

V

C

S

N

L

I

G

C

Q

S

N

H

V

C

F
|
F

V

I

G
x
E

N

N

R

D

V

V

S

K

I

I

G

G

D

N

H

N

C

V

K

T

V

I

Q

K

N

C

N

G

V

V

S

Q

V

I

Y
x
W

D

D

N

G

V

I

T

E

L

I

E

E

E

D

G

D

V

V

F

F

C

I

G

G

P

P

S

N

M

V

V

T

F

F

T
x
C

N
|
N

V
x
D

Y
x
K

N
x
Y

P
|
P

R

R

S

S

L

K

I

-

E

Q

R
x
Y

K

P

D

K

E

E

Y

F

R

S

D

K

T

T

L

I

V

I

K

K

R

K

G

G

A

A

T

S

L

I

G

G

A

A

N

N

C

A

T

T

I

I

V
x
L

C

P

G

G

I

I

T

T

I

I

G

G

E

E

Y

N

A

A

F

M

V

I

G

G

A

A

G

G

A

A

V

I

V

V

T
|
T

Q
x
K

D

D

V

V

S

L

P

P

Y

H

binding [(2~{R},3~{R},4~{S},5~{S},6~{R})-4-acetamido-6-methyl-3,5-bis(oxidanyl)oxan-2-yl] [[(2~{R},3~{S},5~{R})-5-[5-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate : F44 (= F46), E46 (≠ G48), W64 (≠ Y66), N83 (= N85), D84 (≠ V86), Y92 (≠ R95)
binding coenzyme a: C82 (≠ T84), D84 (≠ V86), K85 (≠ Y87), Y86 (≠ N88), P87 (= P89), L114 (≠ V117), T132 (= T135), K133 (≠ Q136)

7s3wA Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-amino-3,6-dideoxy-d- galactose (see paper)
41% identity, 71% coverage: 3:141/195 of query aligns to 2:138/145 of 7s3wA

query
sites
7s3wA

H

H

Y

M

Q

I

H

H

S

K

S

L

A

A

I

D

V

V

D

-

E

Q

G

S

A

K

Q

N

I

I

G

G

E

S

G

G

S

T

R

R

V

I

W

W

H

Q

F

F

V

C

H

V

V

V

C

L

G

P

G

S

A

A

R

I

I

I

G

G

K

E

G

N

V

C

S

N

L

I

G

C

Q

S

N

H

V

C

F
|
F

V

I

G
x
E

N

N

R

D

V

V

S

K

I

I

G

G

D

N

H

N

C

V

K

T

V

I

Q

K

N

C

N

G

V

V

S

Q

V

I

Y

W

D

D

N

G

V

I

T

E

L

I

E

E

E

D

G

D

V

V

F

F

C

I

G

G

P

P

S

N

M

V

V

T

F

F

T
x
C

N

N

V
x
D

Y
x
K

N
x
Y

P
|
P

R

R

S

S

L

K

I

-

E

Q

R
x
Y

K

P

D

K

E

E

Y

F

R

S

D

K

T

T

L

I

V

I

K

K

R

K

G

G

A

A

T

S

L

I

G

G

A

A

N

N

C

A

T

T

I

I

V
x
L

C

P

G

G

I

I

T

T

I

I

G

G

E

E

Y

N

A

A

F

M

V

I

G

G

A

A

G

G

A

A

V

I

V

V

T
|
T

Q
x
K

D

D

V

V

S

L

P

P

Y

H

binding oxidized coenzyme a: C82 (≠ T84), D84 (≠ V86), K85 (≠ Y87), Y86 (≠ N88), P87 (= P89), L114 (≠ V117), T132 (= T135), K133 (≠ Q136)
binding (3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: F44 (= F46), E46 (≠ G48), D84 (≠ V86), Y92 (≠ R95)

7s3uA Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-amino-3,6-dideoxy-d-glucose (see paper)
41% identity, 71% coverage: 3:141/195 of query aligns to 2:138/145 of 7s3uA

query
sites
7s3uA

H

H

Y

M

Q

I

H

H

S

K

S

L

A

A

I

D

V

V

D

-

E

Q

G

S

A

K

Q

N

I

I

G

G

E

S

G

G

S

T

R

R

V

I

W

W

H

Q

F

F

V

C

H

V

V

V

C

L

G

P

G

S

A

A

R

I

I

I

G

G

K

E

G

N

V

C

S

N

L

I

G

C

Q

S

N

H

V

C

F
|
F

V

I

G
x
E

N

N

R

D

V

V

S

K

I

I

G

G

D

N

H

N

C

V

K

T

V

I

Q

K

N

C

N

G

V

V

S

Q

V

I

Y

W

D

D

N

G

V

I

T

E

L

I

E

E

E

D

G

D

V

V

F

F

C

I

G

G

P

P

S

N

M

V

V

T

F

F

T
x
C

N
|
N

V
x
D

Y
x
K

N
x
Y

P
|
P

R

R

S

S

L

K

I

-

E

Q

R
x
Y

K

P

D

K

E

E

Y

F

R

S

D

K

T

T

L

I

V

I

K

K

R

K

G

G

A

A

T

S

L

I

G

G

A

A

N

N

C

A

T

T

I

I

V
x
L

C

P

G

G

I

I

T

T

I

I

G

G

E

E

Y

N

A

A

F

M

V

I

G

G

A

A

G

G

A

A

V

I

V

V

T
|
T

Q
x
K

D

D

V

V

S

L

P

P

Y

H

binding coenzyme a: C82 (≠ T84), N83 (= N85), D84 (≠ V86), K85 (≠ Y87), Y86 (≠ N88), P87 (= P89), L114 (≠ V117), T132 (= T135), K133 (≠ Q136)
binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: F44 (= F46), E46 (≠ G48), D84 (≠ V86), Y92 (≠ R95)

3fsbA Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with coa and dtdp-3-amino-quinovose (see paper)
30% identity, 87% coverage: 17:186/195 of query aligns to 89:244/260 of 3fsbA

query
sites
3fsbA

I

I

G

G

E

D

G

N

S

F

R

Q

V

T

W

G

H

H

F

K

V

V

H

T

V

I

C

R

G

E

G

N

A

T

R

K

I

I

G

G

K

N

G

N

V

V

S

K

L

I

G
|
G

Q

T

N

L

V

S

F

D

V

I

G

Q

N

H

R

H

V

V

S

Y

I

I

G

G

D

N

H

Y

C

V

K

N

V

I

Q
x
H

N

S

N

N

V

V

S

F

V

V

Y

G

D

E

N

K

V

S

T

I

L

I

E

K

E

D

G

F

V

V

F

W

C

L

G
x
F

P

P

S

H

M

V

V

V

F

L

T

T

N

N

V

D

Y

P

N

T

P

P

R

P

S

S

L

-

I

-

E

-

R

-

K

-

D

N

E

E

Y

L

R

L

D

G

T

V

L

T

V

I

K

E

R

L

G

F

A

A

T

V

L

I

G
x
A

A
|
A

N

R

C

S

T

V

I

V

V

L

C

P

G

G

I

I

T

H

I

I

G

N

E

E

Y

D

A

A

F

L

V

V

G
|
G

A
|
A

G

G

A

A

V

V

T

T

Q

K

D

D

V

V

S

P

P

K

Y

E

A

T

L

V

M

V

V
|
V

G

G

V

N

P

P

A

A

R

R

Q

E

I

I

G

C

W

S

M

I

S

R

E

K

Y

I

G

K

E

N

Q

K

L

I

D

-

L

-

P

-

L

-

S

-

G

-

A

T

G

G

E

E

A

Q

L

V

C

Y

P

P

H

W

T

-

G

-

T

-

R

R

Y

Y

V

T

L
x
F

R

K

D
x
R

G

G

binding coenzyme a: F150 (≠ G78), A178 (≠ G111), A179 (= A112), G196 (= G129), A197 (= A130), V212 (= V145)
binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: G114 (= G42), H132 (≠ Q60), F150 (≠ G78), F241 (≠ L183), R243 (≠ D185)

Sites not aligning to the query:

binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: 78

3fscA Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with coa and dtdp-3-amino-fucose (see paper)
30% identity, 87% coverage: 17:186/195 of query aligns to 89:244/259 of 3fscA

query
sites
3fscA

I

I

G

G

E

D

G

N

S

F

R

Q

V

T

W

G

H

H

F

K

V

V

H

T

V

I

C

R

G

E

G

N

A

T

R

K

I

I

G

G

K

N

G

N

V

V

S
x
K

L

I

G
|
G

Q
x
T

N

L

V

S

F

D

V

I

G

Q

N

H

R

H

V

V

S

Y

I

I

G

G

D

N

H

Y

C

V

K

N

V

I

Q
x
H

N
x
S

N

N

V

V

S

F

V

V

Y

G

D

E

N

K

V

S

T

I

L

I

E

K

E

D

G

F

V

V

F

W

C

L

G
x
F

P

P

S

H

M

V

V

V

F

L

T

T

N

N

V

D

Y

P

N

T

P

P

R

P

S

S

L

-

I

-

E

-

R

-

K

-

D

N

E

E

Y

L

R

L

D

G

T

V

L

T

V

I

K

E

R

L

G

F

A

A

T

V

L

I

G
x
A

A
|
A

N

R

C

S

T

V

I

V

V

L

C

P

G

G

I

I

T

H

I

I

G

N

E

E

Y

D

A

A

F

L

V

V

G
|
G

A
|
A

G

G

A

A

V

V

T

T

Q

K

D

D

V

V

S

P

P

K

Y

E

A

T

L

V

M

V

V
|
V

G

G

V

N

P

P

A

A

R

R

Q

E

I

I

G

C

W

S

M

I

S

R

E

K

Y

I

G

K

E

N

Q
x
K

L

I

D

-

L

-

P

-

L

-

S

-

G

-

A

T

G

G

E

E

A

Q

L

V

C

Y

P

P

H

W

T

-

G

-

T

-

R

R

Y

Y

V

T

L

F

R

K

D
x
R

G

G

binding coenzyme a: F150 (≠ G78), A178 (≠ G111), A179 (= A112), G196 (= G129), A197 (= A130), V212 (= V145), K228 (≠ Q161)
binding (3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: K112 (≠ S40), G114 (= G42), T115 (≠ Q43), H132 (≠ Q60), S133 (≠ N61), R243 (≠ D185)

Sites not aligning to the query:

binding (3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: 78

3fs8A Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d- glucose n-acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with acetyl-coa (see paper)
30% identity, 87% coverage: 17:186/195 of query aligns to 89:244/259 of 3fs8A

query
sites
3fs8A

I

I

G

G

E

D

G

N

S

F

R

Q

V

T

W

G

H

H

F

K

V

V

H

T

V

I

C
x
R

G

E

G

N

A

T

R

K

I

I

G

G

K

N

G

N

V

V

S

K

L

I

G

G

Q

T

N

L

V

S

F

D

V

I

G

Q

N

H

R

H

V

V

S

Y

I

I

G

G

D

N

H

Y

C

V

K

N

V

I

Q

H

N

S

N

N

V

V

S

F

V

V

Y

G

D

E

N

K

V

S

T

I

L

I

E

K

E

D

G

F

V

V

F

W

C

L

G
x
F

P
|
P

S

H

M

V

V

V

F

L

T

T

N

N

V

D

Y

P

N

T

P

P

R

P

S

S

L

-

I

-

E

-

R

-

K

-

D

N

E

E

Y

L

R

L

D

G

T

V

L

T

V

I

K

E

R

L

G

F

A

A

T

V

L

I

G
x
A

A
|
A

N

R

C

S

T

V

I

V

V

L

C

P

G

G

I

I

T

H

I

I

G

N

E

E

Y

D

A

A

F

L

V

V

G
|
G

A
|
A

G

G

A

A

V

V

T

T

Q

K

D

D

V

V

S

P

P

K

Y

E

A

T

L

V

M

V

V
|
V

G

G

V

N

P

P

A

A

R

R

Q

E

I

I

G

C

W

S

M

I

S

R

E

K

Y

I

G

K

E

N

Q
x
K

L

I

D

-

L

-

P

-

L

-

S

-

G

-

A

T

G

G

E

E

A

Q

L

V

C

Y

P

P

H

W

T

-

G

-

T

-

R

R

Y

Y

V

T

L

F

R

K

D

R

G

G

binding acetyl coenzyme *a: F150 (≠ G78), P151 (= P79), A178 (≠ G111), A179 (= A112), G196 (= G129), A197 (= A130), V212 (= V145), K228 (≠ Q161)
binding thymine: R102 (≠ C30)

Sites not aligning to the query:

binding thymine: 84

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
29% identity, 59% coverage: 36:150/195 of query aligns to 72:179/190 of 5u2kA

query
sites
5u2kA

G

G

K

W

G

N

V

V

S

K

L

L

G

G

Q

K

N

N

V

V

F

Y

V

V

G

N

N

T

R

N

V

C

S

Y

I

F

G
x
M

D

D

H

G

C

G

K

Q

V

I

Q

-

N

-

V

-

S

-

V

-

Y

-

D

-

N

-

V

-

T

T

L

I

E

G

E

D

G

N

V

V

F

F

C

I

G

G

P

P

S

N

M

C

V

G

F

F

T
x
Y

N

T

V
x
A

Y

T

N
x
H

P

P

R

L

S

N

L

F

I

H

E

H

R

R

K

N

D

E

E

G

Y

F

R

E

D

K

T

A

-

G

-

P

-

I

L

H

V

I

K

G

R

S

G

N

A

T

T

W

L

F

G

G

A

G

N

H

C

V

T

A

I

V

V
x
L

C

P

G

G

I

V

T

T

I

I

G

G

E

E

Y

G

A

S

F

V

V

I

G

G

A

A

G

G

A

S

V

V

T
|
T

Q
x
K

D

D

V

I

S

P

P

P

Y

H

A

S

L

L

M

A

V

V

G

G

V

N

P

P

A

C

R

K

binding coenzyme a: M90 (≠ G54), Y110 (≠ T84), A112 (≠ V86), H114 (≠ N88), L146 (≠ V117), T164 (= T135), K165 (≠ Q136)

3nz2C Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
35% identity, 53% coverage: 48:150/195 of query aligns to 69:176/183 of 3nz2C

query
sites
3nz2C

G

G

N

K

R

T

V

I

S

R

I

I

G

G

D

D

H

H

C

T

K

F

V

I

Q

N

N

M

N

N

V

V

S

V

V

M

Y

L

D

D

N

G

-

A

-

P

V

I

T

T

L

I

E

G

E

D

G

H

V

V

F

L

C

I

G

G

P

P

S

S

M

T

V

Q

F

F

T
x
Y

N

T

V

A

Y

S

N
x
H

P

-

R

-

S

S

L

L

I

D

E

Y

R

R

K

R

D

R

E

Q

Y

A

R

W

D

E

T

T

L

I

-

C

-

K

-

P

-

I

-

V

V

I

K

E

R

D

G

D

A

V

T

W

L

I

G
|
G

A

G

N

N

C

V

T

V

I

I

V

N

C

Q

G

G

I

V

T

T

I

I

G

G

E

A

Y

R

A

S

F

V

V

V

G
x
A

A
|
A

G
x
N

A

S

V

V

T
x
N

Q

Q

D

D

V

V

S

P

P

P

Y

D

A

T

L

L

M

V

V
x
G

G

G

V
x
T

P
|
P

A

A

R

R

binding acetyl coenzyme *a: Y107 (≠ T84), H111 (≠ N88), G137 (= G111), A155 (≠ G129), A156 (= A130), N161 (≠ T135), G171 (≠ V145), T173 (≠ V147), P174 (= P148)
binding magnesium ion: N157 (≠ G131), T173 (≠ V147)

Sites not aligning to the query:

binding acetyl coenzyme *a: 178, 179

3nz2J Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
35% identity, 53% coverage: 48:150/195 of query aligns to 72:179/185 of 3nz2J

query
sites
3nz2J

G

G

N

K

R

T

V

I

S

R

I

I

G

G

D

D

H

H

C

T

K

F

V

I

Q

N

N

M

N

N

V

V

S

V

V

M

Y

L

D

D

N

G

-

A

-

P

V

I

T

T

L

I

E

G

E

D

G

H

V

V

F

L

C

I

G
|
G

P

P

S

S

M

T

V

Q

F

F

T
x
Y

N

T

V

A

Y

S

N
x
H

P

-

R

-

S

S

L

L

I

D

E

Y

R

R

K

R

D

R

E

Q

Y

A

R

W

D

E

T

T

L

I

-

C

-

K

-

P

-

I

-

V

V

I

K

E

R

D

G

D

A

V

T
x
W

L

I

G
|
G

A

G

N

N

C

V

T

V

I

I

V

N

C

Q

G

G

I

V

T

T

I

I

G

G

E

A

Y

R

A

S

F

V

V

V

G
x
A

A
|
A

G

N

A

S

V

V

T
x
N

Q

Q

D

D

V

V

S

P

P

P

Y

D

A

T

L

L

M

V

V
x
G

G

G

V
x
T

P
|
P

A

A

R

R

binding acetyl coenzyme *a: G104 (= G78), Y110 (≠ T84), H114 (≠ N88), W138 (≠ T109), G140 (= G111), A158 (≠ G129), A159 (= A130), N164 (≠ T135), G174 (≠ V145), T176 (≠ V147), P177 (= P148)

Sites not aligning to the query:

binding acetyl coenzyme *a: 182

3ectA Crystal structure of the hexapeptide-repeat containing- acetyltransferase vca0836 from vibrio cholerae
35% identity, 53% coverage: 48:150/195 of query aligns to 62:169/176 of 3ectA

query
sites
3ectA

G

G

N

K

R

T

V

I

S

R

I

I

G

G

D

D

H

H

C

T

K

F

V

I

Q

N

N

M

N

N

V

V

S

V

V

M

Y

L

D

D

N

G

-

A

-

P

V

I

T

T

L

I

E

G

E

D

G

H

V

V

F

L

C

I

G

G

P

P

S

S

M

T

V

Q

F

F

T

Y

N

T

V

A

Y

S

N

H

P

-

R

-

S

S

L

L

I

D

E

Y

R

R

K

R

D

R

E

Q

Y

A

R

W

D

E

T

T

L

I

-

C

-

K

-

P

-

I

-

V

V

I

K

E

R

D

G

D

A

V

T

W

L

I

G

G

A

G

N

N

C

V

T

V

I

I

V

N

C

Q

G
|
G

I

V

T

T

I

I

G

G

E

A

Y

R

A

S

F

V

V

V

G

A

A

A

G

N

A

S

V

V

T

N

Q

Q

D
|
D

V

V

S

P

P

P

Y

D

A

T

L

L

M

V

V

G

G

G

V

T

P

P

A

A

R

R

binding calcium ion: G138 (= G119), D156 (= D137)

4h7oA Crystal structure of serine acetyltransferase from vibrio cholerae o1 biovar el tor n16961
30% identity, 71% coverage: 27:164/195 of query aligns to 138:252/258 of 4h7oA

query
sites
4h7oA

V

V

H

D

V

I

C

H

G

P

G

A

A

A

R

R

I

I

G

G

K

R

G

G

V

I

S

M

L

L

G
x
D

Q
x
H

N

-

V

-

F

-

V

-

G

A

N

T

R

G

V

I

S

V

I

I

G

G

D

E

H

T

C

A

K

V

V

V

Q

E

N

D

V

V

S

S

V

I

Y

L

D

Q

N

D

V

V

T

T

L

L

E

G

-
x
G

E

T

G

G

V

K

F

E

C

C

G

G

P

D

S

-

M

-

V

-

F

-

T

-

N

-

V

-

Y

-

N

-

P

-

R

-

S

-

L

-

I

-

E

-

R

-

K

-

D

-

E

-

Y

-

R
|
R

D
x
H

T

P

L

K

V

I

K

R

R

E

G

G

A

V

T

M

L

I

G

G

A

A

N

G

C

A

T

K

I

I

V

L

C

G

G

N

I

I

T

E

I

V

G

G

E

E

Y

G

A

A

F

K

V

I

G

G

A

S

G

G

A

S

V

V

T

L

Q

Q

D

A

V

V

S

P

P

P

Y

H

A

T

L

T

M

V

V

A

G

G

V

V

P

P

A

A

R

R

Q

I

I

V

G

G

W

R

M

P

S

Q

E

S

Y

D

G

K

E

P

Q

S

L

L

D

D

L

M

binding cysteine: D153 (≠ G42), H154 (≠ Q43), G180 (vs. gap), R188 (= R100), H189 (≠ D101)

Sites not aligning to the query:

binding arginine: 51, 53, 127, 131
binding cysteine: 88, 89

3st8A Crystal structure of glmu from mycobacterium tuberculosis in complex with coenzyme a, glucosamine 1-phosphate and uridine-diphosphate-n- acetylglucosamine
35% identity, 72% coverage: 15:155/195 of query aligns to 324:456/475 of 3st8A

query
sites
3st8A

A

S

Q

S

I

I

G

G

E

D

G

G

S

A

R

A

V

V

W

G

H

P

F

F

V

T

H

Y

V

L

C

R

G

P

G

G

A

T

R

A

I

L

G

G

K

A

G

D

V

G

S

K

L

L

G

G

Q

A

N

F

V

V

F

E

V

V

G

K

N

N

R

S

V

-

S

T

I

I

G

G

D

T

H

G

C

T

K

K

V

V

Q
x
P

N
x
H

N

L

V

T

S

Y

V

V

Y

G

D

D

N

-

V

A

T

D

L

I

E

G

E

E

G

Y

V

S

F
x
N

C

I

G
|
G

P
x
A

S

S

M

S

V

V

F

F

T

V

N

N

V

-

Y

Y

N

D

P

G

R

T

S

S

L

-

I

-

E

-

R

-

K

-

D

-

E

-

Y

K

R

R

D

R

T

T

L

T

V

V

K

G

R

S

G

H

A

V

T

R

L

T

G
|
G

A
x
S

N

D

C

T

T

M

I

F

V

V

C

A

G

P

I

V

T

T

I

I

G

G

E

D

Y

G

A

A

F

Y

V

T

G
|
G

A
|
A

G

G

A

T

V

V

T

R

Q

E

D

D

V

V

S

P

P

P

Y

G

A

A

L

L

M

A

V
|
V

G

S

V
x
A

-

G

P

P

A

Q

R

R

Q

N

I

I

-

E

G

N

W

W

M

V

binding coenzyme a: G385 (= G78), A386 (≠ P79), G410 (= G111), S411 (≠ A112), G428 (= G129), A429 (= A130), V444 (= V145), A446 (≠ V147)
binding 2-amino-2-deoxy-1-O-phosphono-alpha-D-glucopyranose: P368 (≠ Q60), H369 (≠ N61), N383 (≠ F76)

Sites not aligning to the query:

active site: 14
binding magnesium ion: 109, 234
binding uridine-diphosphate-n-acetylglucosamine: 7, 8, 9, 10, 78, 81, 83, 84, 87, 109, 145, 146, 161, 176, 206, 234

3sptA Crystal structure of glmu from mycobacterium tuberculosis in complex with acetyl coenzyme a and uridine-diphosphate-n-acetylglucosamine
35% identity, 72% coverage: 15:155/195 of query aligns to 324:456/474 of 3sptA

query
sites
3sptA

A

S

Q

S

I

I

G

G

E

D

G

G

S

A

R

A

V

V

W

G

H

P

F

F

V

T

H

Y

V

L

C

R

G

P

G

G

A

T

R

A

I

L

G

G

K

A

G

D

V

G

S

K

L

L

G

G

Q

A

N

F

V

V

F

E

V

V

G

K

N

N

R

S

V

-

S

T

I

I

G

G

D

T

H

G

C

T

K

K

V

V

Q

P

N

H

N

L

V

T

S

Y

V

V

Y

G

D

D

N

-

V

A

T

D

L

I

E

G

E

E

G

Y

V

S

F

N

C

I

G
|
G

P
x
A

S

S

M

S

V

V

F

F

T

V

N

N

V

-

Y

Y

N

D

P

G

R

T

S

S

L

-

I

-

E

-

R

-

K

-

D

-

E

-

Y

K

R

R

D

R

T

T

L

T

V

V

K

G

R

S

G

H

A

V

T

R

L

T

G
|
G

A
x
S

N

D

C

T

T

M

I

F

V

V

C

A

G

P

I

V

T

T

I

I

G

G

E

D

Y

G

A

A

F

Y

V

T

G
|
G

A
|
A

G

G

A

T

V

V

T

R

Q

E

D

D

V

V

S

P

P

P

Y

G

A

A

L

L

M

A

V
|
V

G

S

V
x
A

-

G

P

P

A

Q

R

R

Q

N

I

I

-

E

G

N

W

W

M

V

binding acetyl coenzyme *a: G385 (= G78), A386 (≠ P79), G410 (= G111), S411 (≠ A112), G428 (= G129), A429 (= A130), V444 (= V145), A446 (≠ V147)

Sites not aligning to the query:

active site: 14
binding magnesium ion: 109, 234
binding uridine-diphosphate-n-acetylglucosamine: 7, 8, 9, 10, 78, 81, 83, 84, 87, 109, 145, 146, 161, 176, 206, 234

Query Sequence

>N515DRAFT_4259 FitnessBrowser__Dyella79:N515DRAFT_4259
MAHYQHSSAIVDEGAQIGEGSRVWHFVHVCGGARIGKGVSLGQNVFVGNRVSIGDHCKVQ
NNVSVYDNVTLEEGVFCGPSMVFTNVYNPRSLIERKDEYRDTLVKRGATLGANCTIVCGI
TIGEYAFVGAGAVVTQDVSPYALMVGVPARQIGWMSEYGEQLDLPLSGAGEALCPHTGTR
YVLRDGRVRREEVTA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory