SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing N515DRAFT_4319 N515DRAFT_4319 electron transfer flavoprotein alpha subunit apoprotein to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 11 hits to proteins with known functional sites (download)

1efpA Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
55% identity, 99% coverage: 4:313/314 of query aligns to 2:307/307 of 1efpA

query
sites
1efpA

I

V

L

L

V

L

A

G

E

E

H

V

L

T

N

N

G

G

K

A

L

L

N

N

S

R

S

D

T

A

A

T

R

A

A

K

V

A

S

V

A

A

A

V

A

K

V

A

K

L

G

G

D

D

S

-

I

V

D

T

V

V

L

L

V

C

L

A

A

G

D

A

S

S

V

A

D

K

A

A

I

A

A

A

A

E

E

E

A

A

K

K

I

I

D

A

G

G

V

V

G

A

K

K

V

V

L

L

T

V

I

A

A

E

R

D

A

A

E

L

N

Y

A

G

H

H

P

R

L

L

A

A

A

E

V

P

L

T

A

A

P

A

Q

L

V

I

A

V

K

G

A

L

A

A

A

G

G

D

Y

Y

S

S

H

H

V

I

F

A

P

P

S

A

T

T

F

D

G

A

K

K

D

N

V

V

A

M

P

P

R

R

V

V

A

A

L

L

G

D

V

V

S

M

Q

V

V

L

S

S

D

D

V

V

M

S

S

A

V

I

E

L

G

D

A

A

H

D

S

T

F

F

K

E

R

R

P

P

I

I

Y

Y

A

A

G

G

N

N

A

A

I

I

V

Q

T

V

V

V

E

K

A

S

N

K

P

D

A

A

H

K

A

K

V

V

V

F

A

-

T

T

I

I

R

R

T

T

A

A

S

S

W

F

P

D

A

A

G

G

S

E

R

G

G

G

D

T

S

A

A

P

A

V

V

T

E

E

A

T

L

A

A

A

V

-

D

-

A

A

L

D

P

P

A

G

H

L

T

S

R

S

F

W

V

V

E

A

L

D

Q

E

Q

V

G

A

K

E

S

S

D

D

R

R

P

P

D

E

L

L

Q

T

G

S

A

A

G

R

K

R

V

V

S

S

G

G

G
|
G

R
|
R

G
|
G

V

L

G

G

S

S

K

K

E

E

N

S

F

F

E

A

V

I

I

I

Y

E

K

E

L

L

A

A

D

D

K

K

I

L

G

G

A

A

V

V

G

G

A

A

S
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S

R
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R

A
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A

A

A

V

V

D

D

A

S

G

G

Y

Y

V

A

P

P

N

N

E

D

L

W

Q
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Q

V
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V

G
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G

Q

Q

T
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T

G

G

K

K

I

V

A

A

P

P

E

E

L

L

Y

Y

M

V

A

A

F

V

G
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G

I
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I

S
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S

G

G

A
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A

I

I

Q
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Q

H
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H

L

L

T

A

G

G

I

M

K

K

D

D

A

S

G

K

T

V

I

I

V

V

A

A

I

I

N
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N

K
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K

D

D

G

E

E

E

A

A

P

P

I

I

F

F

E

Q

I

I

A

A

D

D

I

Y

G

G

L

L

V

V

G

G

D
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D

L
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L

F

F

K

S

L

V

I

V

P

P

E

E

I

L

E

T

Q

G

A

K

L

L

binding flavin-adenine dinucleotide: G199 (= G205), R200 (= R206), G201 (= G207), S225 (= S231), R226 (= R232), A227 (= A233), Q239 (= Q245), V240 (= V246), G241 (= G247), T243 (= T249), G256 (= G262), I257 (= I263), S258 (= S264), A260 (= A266), Q262 (= Q268), H263 (= H269), N277 (= N283), K278 (= K284), D295 (= D301), L296 (= L302)

2a1uA Crystal structure of the human etf e165betaa mutant (see paper)
47% identity, 98% coverage: 5:313/314 of query aligns to 5:312/315 of 2a1uA

query
sites
2a1uA

L

L

V

V

V

I

A

A

E

E

H

H

L

A

N

N

G

D

K

S

L

L

N

A

S

P

S

I

T

T

A

L

R

N

A

T

V

I

S

T

A

A

A

T

A

R

V

L

K

G

G

G

D

E

S

-

I

V

D

S

V

C

L

L

V

V

L

A

A

G

D

T

S

K

V

C

D

D

A

K

I

V

A

A

A

Q

E

D

A

L

A

C

K

K

I

V

D

A

G

G

V

I

G

A

K

K

V

V

L

L

T

V

I

A

A

Q

R

H

A

D

E

V

N

Y

A

K

H

G

P

L

L

L

A

P

A

E

V

E

L

L

A

T

P

P

Q

L

V

I

-

L

-

A

A

T

K

Q

A

K

A

Q

A

F

G

N

Y

Y

S

T

H

H

V

I

F

C

F

A

P

G

S

A

T

S

T

A

F

F

G

G

K

K

D

N

V

L

A

L

P

P

R

R

V

V

A

A

L

K

L

L

G

E

V

V

S

A

Q

P

V

I

S

S

D

D

V

I

M

I

S

A

V

I

E

K

G

S

A

P

H

D

S

T

F

F

K

V

R

R

P

T

I

I

Y

Y

A

A

G

G

N

N

A

A

I

L

V

C

T

T

V

V

E

K

A

C

N

D

P

-

A

E

H

K

A

V

V

K

V

V

A

F

T

S

I

V

R

R

T

G

A

T

S

S

W

F

P

D

A

A

G

A

S

T

R

S

G

G

D

G

S

S

A

A

A

S

V

S

E

E

A

K

L

A

A

S

V

S

D

T

A

S

A

P

L

V

P

E

A

I

H

-

T

S

R

E

F

W

V

L

E

D

L

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Q

K

Q

L

G

T

K

K

S

S

D

D

R

R

P

P

D

E

L

L

Q

T

G

G

A

A

G

K

K

V

V

V

S

S

G

G

G
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G

R
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R

G

G

V

L

G

K

S

S

K

G

E

E

N

N

F

F

E

K

V

L

I

L

Y

Y

K

D

L

L

A

A

D

D

K

Q

I

L

G

H

A

A

V

V

G

G

A

A

S
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S

R
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R

A
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A

A

A

V

V

D

D

A

A

G

G

Y

F

V

V

P

P

N

N

E

D

L

M

Q
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Q

V
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V

G
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G

Q

Q

T
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T

G

G

K

K

I

I

I

V

A

A

P

P

E

E

L

L

Y

Y

M

I

A

A

F

V

G
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G

I
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I

S
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S

G

G

A
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A

I

I

Q
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Q

H
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H

L

L

T

A

G

G

I

M

K

K

D

D

A

S

G

K

T

T

I

I

V

V

A

A

I

I

N
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N

K
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K

D

D

G

P

E

E

A

A

P

P

I

I

F

F

E

Q

I

V

A

A

D

D

I

Y

G

G

L

I

V

V

G

A

D
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D

L
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L

F

F

K

K

L

V

I

V

P

P

E

E

I

M

E

T

Q

E

A

I

L

L

binding flavin-adenine dinucleotide: G204 (= G205), R205 (= R206), S230 (= S231), R231 (= R232), A232 (= A233), Q244 (= Q245), V245 (= V246), G246 (= G247), T248 (= T249), G261 (= G262), I262 (= I263), S263 (= S264), A265 (= A266), Q267 (= Q268), H268 (= H269), N282 (= N283), K283 (= K284), D300 (= D301), L301 (= L302)

P13804 Electron transfer flavoprotein subunit alpha, mitochondrial; Alpha-ETF from Homo sapiens (Human) (see 6 papers)
47% identity, 98% coverage: 5:313/314 of query aligns to 23:330/333 of P13804

query
sites
P13804

L
|
L

V
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V

V
x
I

A
|
A

E
|
E

H
|
H

L
x
A

N
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N

G
x
D

K
x
S

L
|
L

N
x
A

S
x
P

S
x
I

T
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T

A
x
L

R
x
N

A
x
T

V
x
I

S
x
T

A
|
A

A
x
T

A
x
R

V
x
L

K
x
G

G
|
G

D
x
E

S

-

I
x
V

D
x
S

V
x
C

L
|
L

V
|
V

L
x
A

A
x
G

D
x
T

S
x
K

V
x
C

D
|
D

A
x
K

I
x
V

A
|
A

A
x
Q

E
x
D

A
x
L

A
x
C

K
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K

I
x
V

D
x
A

G
|
G

V
x
I

G
x
A

K
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K

V
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V

L
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L

T
x
V

I
x
A

A
x
Q

R
x
H

A
x
D

E
x
V

N
x
Y

A
x
K

H
x
G

P
x
L

L
|
L

A
x
P

A
x
E

V
x
E

L
|
L

A
x
T

P
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P

Q
x
L

V
x
I

-
x
L

-
x
A

A
x
T

K
x
Q

A
x
K

A
x
Q

A
x
F

G
x
N

Y
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Y

S
x
T

H
|
H

V
x
I

F
x
C

F
x
A

P
x
G

S
x
A

T
x
S

T
x
A

F
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F

G
|
G

K
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K

D
x
N

V
x
L

A
x
L

P
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P

R
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R

V
|
V

A
|
A

L
x
K

L
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L

G
x
E

V
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V

S
x
A

Q
x
P

V
x
I

S
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S

D
|
D

V
x
I

M
x
I

S
x
A

V
x
I

E
x
K

G
x
S

A
x
P

H
x
D

S
x
T

F
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F

K
x
V

R
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R

P
x
T

I
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I

Y
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Y

A
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A

G
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G

N
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N

A
|
A

I
x
L

V
x
C

T
|
T

V
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V

E
x
K

A
x
C

N
x
D

P

-

A
x
E

H
x
K

A
x
V

V
x
K

V
|
V

A
x
F

T
x
S

I
x
V

R
|
R

T
x
G

A
x
T

S
|
S

W
x
F

P
x
D

A
|
A

G
x
A

S
x
T

R
x
S

G
|
G

D
x
G

S
|
S

A
|
A

A
x
S

V
x
S

E
|
E

A
x
K

L
x
A

A
x
S

V
x
S

D
x
T

A
x
S

A
x
P

L
x
V

P
x
E

A
x
I

H

-

T
x
S

R
x
E

F
x
W

V
x
L

E
x
D

L
x
Q

Q
x
K

Q
x
L

G
x
T

K
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K

S
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S

D
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D

R
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R

P
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P

D
x
E

L
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L

Q
x
T

G
|
G

A
|
A

G
x
K

K
x
V

V
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V

S
|
S

G
|
G

R
|
R

G
|
G

V
x
L

G
x
K

S
|
S

K
x
G

E
|
E

N
|
N

F
|
F

E
x
K

V
x
L

I
x
L

Y
|
Y

K
x
D

L
|
L

A
|
A

D
|
D

K
x
Q

I
x
L

G
x
H

A
|
A

V
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V

G
|
G

A
|
A

S
|
S

R
|
R

A
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A

V
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V

D
|
D

A
|
A

G
|
G

Y
x
F

V
|
V

P
|
P

N
|
N

E
x
D

L
x
M

Q
|
Q

V
|
V

G
|
G

Q
|
Q

T
|
T

G
|
G

K
|
K

I
|
I

I
x
V

A
|
A

P
|
P

E
|
E

L
|
L

Y
|
Y

M
x
I

A
|
A

F
x
V

G
|
G

I
|
I

S
|
S

G
|
G

A
|
A

I
|
I

Q
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Q

H
|
H

L
|
L

T
x
A

G
|
G

I
x
M

K
|
K

D
|
D

A
x
S

G
x
K

T
|
T

I
|
I

V
|
V

A
|
A

I
|
I

N
|
N

K
|
K

D
|
D

G
x
P

E
|
E

A
|
A

P
|
P

I
|
I

F
|
F

E
x
Q

I
x
V

A
|
A

D
|
D

I
x
Y

G
|
G

L
x
I

V
|
V

G
x
A

D
|
D

L
|
L

F
|
F

K
|
K

L
x
V

I
x
V

P
|
P

E
|
E

I
x
M

E
x
T

Q
x
E

A
x
I

L
|
L

G116 (= G97) to R: in GA2A; impaired protein stability and loss of electron transfer activity; dbSNP:rs119458971
T171 (≠ A153) to I: decreased protein stability; dbSNP:rs1801591
R223 (= R206) binding
S248 (= S231) binding
R249 (= R232) mutation to A: Loss of electron transfer activity.
VGQT 263:266 (= VGQT 246:249) binding
T266 (= T249) to M: in GA2A; decreased electron transfer activity; dbSNP:rs119458970
SGAIQH 281:286 (= SGAIQH 264:269) binding
N300 (= N283) binding
DL 318:319 (= DL 301:302) binding

Sites not aligning to the query:

20:204 Domain I
205:333 Domain II

6fahE Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
38% identity, 72% coverage: 89:313/314 of query aligns to 157:388/393 of 6fahE

query
sites
6fahE

V

V

F

L

F

Y

P

G

S

A

T

T

T

H

F

I

G

G

K

R

D

D

V

L

A

A

P

P

R

C

V

L

A

A

A

V

L

K

L

V

G

N

V

T

S

G

Q
x
L

V

T

S

A

D

D

V

C

M

T

S

K

V

L

E

E

G

I

A

D

H

P

S

D

F

D

K

K

-

K

-

I

-

R

-

Q

-

T

R
|
R

P

P

I

A

Y

F

A

G

G

G

N

N

A

L

I

M

V

A

T

T

V

I

E

V

A

C

N

P

P

G

A

S

H

R

A

P

V

Q

V

M

A

S

T

T

I

V

R

R

T

P

A

G

S

V

W

M

P

D

A

K

A

A

G

-

S

A

R

Y

G

D

D

P

S

S

A

Q

A

K

V

G

E

E

A

V

L

I

A

K

V

L

D

D

A

A

A

T

L

F

P

N

A

E

-

G

-

D

-

I

H

R

T

T

R

K

F

V

V

L

E

E

L

I

Q

V

Q

K

G

T

K

T

S

T

D

D

R

N

P

I

D

S

L

I

Q

S

G

D

A

A

G

D

K

F

V

I

V

V

S

S

G

G

R
x
M

G
|
G

V

L

G

G

S

K

K

P

E

E

N

G

F

F

E

E

V

L

I

L

Y

K

K

Q

L

L

A

A

D

D

K

K

I

L

G

G

A

G

A

T

V

V

G

A

A

T

S

S

R
|
R

A
|
A

A

C

V

V

D

D

A

A

G

G

Y

W

V

A

P

D

N

H

E

A

L

Q

Q
|
Q

V
|
V

G
|
G

Q
|
Q

T
|
T

G

G

K

T

I

T

I

V

A

K

P

P

E

Q

L

I

Y

Y

M

F

A

A

F

C

G
|
G

I
|
I

S
|
S

G

G

A

A

I

I

Q
|
Q

H
|
H

L

I

T

A

G

G

I

M

K

Q

D

D

A

S

G

D

T

I

I

I

V

I

A

A

I

I

N
|
N

K
|
K

D

N

G

E

E

N

A

A

P

P

I

I

F

F

E

E

I

V

A

A

D

D

I

Y

G

G

L

I

V

V

G

G

D

D

L
|
L

F

Y

K

K

L

V

I

I

P

P

E

A

I

I

E

I

Q

E

A

E

L

L

binding flavin-adenine dinucleotide: L180 (≠ Q112), R200 (= R127), M281 (≠ R206), G282 (= G207), R307 (= R232), A308 (= A233), Q320 (= Q245), V321 (= V246), G322 (= G247), Q323 (= Q248), T324 (= T249), G337 (= G262), I338 (= I263), S339 (= S264), Q343 (= Q268), H344 (= H269), N358 (= N283), K359 (= K284), L377 (= L302)

Sites not aligning to the query:

binding iron/sulfur cluster: 7, 10, 13, 17, 18, 35, 36, 37, 38, 41, 45, 49

5ol2A The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
38% identity, 70% coverage: 89:309/314 of query aligns to 93:320/331 of 5ol2A

query
sites
5ol2A

V

V

F

L

F

F

P

G

S

A

T

T

T

S

F

I

G

G

K

R

D

D

V

L

A

A

P

P

R

R

V

V

A

S

A

A

L

R

L

I

G

H

V

T

S

G

Q

L

V
x
T

S

A

D

D

V

C

M

T

S

G

V

L

E

A

G

V

A

A

H

E

S

D

F

T

K

K

-

L

-

M

-

T

R
|
R

P

P

I

A

Y

F

A

G

G

G

N

N

A

I

I

M

V

A

T

T

V
x
I

E

V

A

C

N

K

P

D

A

F

H

R

A

P

V

Q

V

M

A

S

T

T

I

V

R

R

T

-

A

-

S

-

W

-

P

P

A

G

A

V

G

M

S

K

R

K

G

N

D

E

-

P

-

D

-

E

-

T

-

K

S

E

A

A

A

V

V

I

E

N

A

R

L

F

A

K

V

V

D

E

-

F

A

N

A

D

L

A

P
x
D

A

K

H

L

T

V

R

Q

F

V

V

V

E

Q

L

V

Q

I

Q

K

G

E

K

A

S

K

D

K

R

Q

P

V

D

K

L

I

Q

E

G

D

A

A

G

K

K

I

V

L

V

V

S

S

G

A

G
|
G

R
|
R

G
|
G

V

M

G

G

S

G

K

K

E

E

N

N

F

L

E

D

V

I

I

L

Y

Y

K

E

L

L

A

A

D

E

K

I

I

I

G

G

A

G

A

E

V

V

G

S

A

G

S
|
S

R
|
R

A
|
A

A

T

V

I

D

D

A

A

G

G

Y

W

V

L

P

D

N

K

E

A

L

R

Q
|
Q

V
|
V

G
|
G

Q

Q

T
|
T

G

G

K

K

I

T

I

V

A

R

P

P

E

D

L

L

Y

Y

M

I

A

A

F

C

G
|
G

I
|
I

S
|
S

G

G

A
|
A

I

I

Q
|
Q

H
|
H

L

I

T

A

G

G

I

M

K

E

D

D

A

A

G

E

T

F

I

I

V

V

A

A

I

I

N
|
N

K
|
K

D

N

G

P

E

E

A

A

P

P

I

I

F

F

E

K

I

Y

A

A

D

D

I

V

G

G

L

I

V

V

G

G

D
|
D

L
x
V

F

H

K

K

L

V

I

L

P

P

E

E

I

L

binding calcium ion: D188 (≠ P177)
binding flavin-adenine dinucleotide: T117 (≠ V113), R136 (= R127), I147 (≠ V138), G216 (= G205), R217 (= R206), G218 (= G207), S242 (= S231), R243 (= R232), A244 (= A233), Q256 (= Q245), V257 (= V246), G258 (= G247), T260 (= T249), G273 (= G262), I274 (= I263), S275 (= S264), A277 (= A266), Q279 (= Q268), H280 (= H269), N294 (= N283), K295 (= K284), D312 (= D301), V313 (≠ L302)

Sites not aligning to the query:

binding calcium ion: 75

4kpuA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
38% identity, 70% coverage: 87:307/314 of query aligns to 100:326/338 of 4kpuA

query
sites
4kpuA

S

S

H

S

V

V

F

F

F

I

P

G

S

A

T

T

T

N

F

D

G

G

K

R

D

D

V

L

A

G

P

P

R

R

V

I

A

A

L

R

L

V

G

N

V

T

S

G

Q
x
L

V

C

S

A

D

D

V

C

M

T

S

I

V

L

E

D

-

A

-

E

-

D

G

G

A

L

H

I

S

E

F

W

K

T

R
|
R

P

P

I

A

Y

A

A

G

G

G

N

N

A

I

I

M

V

A

T

T

V
x
I

E

L

A

C

N

K

P

E

A

H

H

R

A

P

V

Q

V

M

A

G

T

T

I

V

R

R

T

P

A

K

S

T

W

F

P

K

A

A

A

M

-

E

-

P

-

D

G

A

S

S

R

R

G

T

D

G

S

E

A

V

A

I

V

N

E

Y

A

T

L

L

A

K

-

N

-

H

V

V

D

D

A

D

A

R

L

V

P

T

A

C

H

I

T

R

R

R

F

E

V

E

E

V

L

V

Q

S

Q

E

G

G

K

E

S

M

D

A

R

I

P

D

D

D

L

-

Q

-

G

-

A

A

G

P

K

F

V

V

V

C

S

S

G

G

G
|
G

R
|
R

G
|
G

V

M

G

K

S

A

K

K

E

E

N

N

F

F

E

S

V

L

I

L

Y

Y

K

D

L

L

A

A

D

H

K

A

I

L

G

G

A

G

A

A

V

V

G

G

A

G

S
|
S

R
|
R

A
|
A

A

A

V

V

D

D

A

E

G

G

Y

F

V

I

P

E

N

H

E

P

L

R

Q
|
Q

V
|
V

G
|
G

Q
|
Q

T
x
S

G

G

K

K

I

T

I

V

A

T

P

P

E

K

L

I

Y

Y

M

F

A

A

F

C

G
|
G

I
|
I

S
|
S

G

G

A
x
S

I

V

Q
|
Q

H
|
H

L

K

T

A

G

G

I

M

K

S

D

K

A

S

G

D

T

T

I

I

V

V

A

C

I

I

N
|
N

K
|
K

D

D

G

P

E

D

A

A

P

P

I

M

F

F

E

E

I

I

A

S

D

K

I

Y

G

G

L

I

V

V

G

G

D
|
D

L
x
A

F

L

K

K

L

I

I

L

P

P

binding flavin-adenine dinucleotide: L125 (≠ Q112), R144 (= R127), I155 (≠ V138), G224 (= G205), R225 (= R206), G226 (= G207), S250 (= S231), R251 (= R232), A252 (= A233), Q264 (= Q245), V265 (= V246), G266 (= G247), Q267 (= Q248), S268 (≠ T249), G281 (= G262), I282 (= I263), S283 (= S264), S285 (≠ A266), Q287 (= Q268), H288 (= H269), N302 (= N283), K303 (= K284), D320 (= D301), A321 (≠ L302)

7qh2A Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
31% identity, 97% coverage: 4:309/314 of query aligns to 13:327/337 of 7qh2A

query
sites
7qh2A

I

I

L

T

V

V

V

Y

A

V

E

D

H

H

L

I

N

E

G

G

K

Q

L

I

N

H

S

P

S

V

T

T

A

F

R

E

A

L

V

I

S

G

A

K

A

A

-

R

-

E

-

L

A

A

V

V

K

I

G

G

D

H

S

P

I

V

D

Y

V

A

L

L

V

L

L

M

A

G

D

T

S

N

V

I

D

T

A

E

I

K

A

A

A

D

E

E

A

L

K

K

I

Y

D

-

G

G

V

V

G

D

K

K

V

V

L

F

T

V

I

Y

A

D

R

K

A

P

E

E

N

L

A

K

H

H

-

F

-

V

-

I

-

E

P

P

L

Y

A

A

A

N

V

V

L

L

A

E

P

D

Q

F

V

I

A

E

K

K

A

V

A

K

A

P

G

-

Y

-

S

S

H

S

V

I

F

L

F

V

P

G

S

A

T

T

T

N

F

V

G

G

K

R

D

S

V

L

A

A

P

P

R

R

V

V

A

A

L

R

L

Y

G

R

V

T

S

G

Q
x
L

V
x
T

S

A

D

D

V

C

M

T

S

I

V

L

E

E

G

M

A

K

H

E

S

N

F

T

K

D

-

L

-

V

-

Q

-

I

R
|
R

P

P

I

A

Y

F

A

G

G

G

N

N

A

I

I

M

V

A

T

Q

V
x
I

E

V

A

T

N

E

P

N

A

T

H

R

A

P

V

Q

V

F

A

C

T

T

I

V

R

R

T

Y

A

K

S

V

W

F

P

T

A

A

A

P

G

E

S

R

R

V

G

N

D

E

S

P

A

W

A

G

-

D

V

V

E

E

A

M

L

M

A

D

V

I

D

E

A

K

A

A

-

K

L

L

P

V

A

S

H

A

T

I

R

E

F

V

V

M

E

E

L

V

Q

I

Q

K

G

-

K

K

S

E

D

K

R

G

P

I

D

D

L

L

Q

S

G

E

A

A

G

E

K

T

V

I

V

V

S

A

G

V

G

G

R
|
R

G
|
G

V

V

G

K

S

C

K

E

E

K

N

D

F

L

E

D

V

M

I

I

Y

H

K

E

L

F

A

A

D

E

K

K

I

I

G

G

A

A

A

T

V

V

G

A

A

C

S
x
T

R
|
R

A

P

A

G

V

I

D

E

A

A

G

G

Y

W

V

F

P

D

N

A

E

R

L

L

Q
|
Q

V
x
I

G
|
G

Q
x
L

T
x
S

G

G

K

R

I

T

I

V

A

K

P

P

E

K

L

L

Y

I

M

I

A

A

F

L

G
|
G

I
|
I

S
|
S

G

G

A

A

I

V

Q
|
Q

H

F

L

A

T

A

G

G

I

M

K

Q

D

N

A

S

G

E

T

Y

I

I

V

I

A

A

I

I

N
|
N

K
x
S

D
|
D

G

P

E

K

A

A

P

P

I

I

F

F

E

N

I

I

A

A

D

H

I

C

G

G

L

M

V

V

G

G

D
|
D

L
|
L

F

Y

K

E

L

I

I

L

P

P

E

E

I

L

binding flavin-adenine dinucleotide: L125 (≠ Q112), T126 (≠ V113), R144 (= R127), I155 (≠ V138), R224 (= R206), G225 (= G207), T249 (≠ S231), R250 (= R232), Q263 (= Q245), I264 (≠ V246), G265 (= G247), L266 (≠ Q248), S267 (≠ T249), G280 (= G262), I281 (= I263), S282 (= S264), Q286 (= Q268), N301 (= N283), S302 (≠ K284), D303 (= D285), D319 (= D301), L320 (= L302)

5ow0A Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
39% identity, 73% coverage: 86:313/314 of query aligns to 69:291/292 of 5ow0A

query
sites
5ow0A

Y

Y

S

E

H

L

V

I

F

L

P

S

S

T

T

T

T

A

F

I

G

G

K

S

D

G

V

L

A

A

P

G

R

P

V

L

A

A

A

V

L

S

L

L

G

G

V

A

S

P

Q

I

V

L

S

S

D

E

V

V

M

T

S

A

V

I

E

S

G

P

A

D

H

L

S

T

F

I

K

E

R

R

P

S

I

L

Y

Y

A

G

G

S

N

K

A

A

I

V

-

A

-

R

V

Y

T

K

V

L

E

E

A

S

N

G

P

P

A

-

H

-

A

-

V

L

V

V

A

L

T

T

I

I

R

K

T

R

A

K

S

Y

W

F

P

E

A

A

G

T

S

L

R

E

G

G

D

-

S

T

A

T

A

A

V

T

E

E

A

E

L

L

A

P

V

V

D

G

A

-

L

-

P

P

A

Q

H

K

T

I

R

T

F

L

V

L

E

E

L

E

Q

I

Q

E

G

E

K

E

S

R

D

T

R

G

P

I

D

P

L

L

Q

E

G

D

A

A

G

E

K

V

V

V

S

T

G

G

G
|
G

R
|
R

G
|
G

V

I

G

G

S

S

K

G

E

D

N

N

F

F

E

S

V

I

I

L

Y

K

K

E

L

I

A

A

D

G

K

M

I

L

G

N

A

G

A

A

V

V

G

G

A

A

S
|
S

R
|
R

A

G

A

A

V

V

D

D

A

E

G

G

Y

W

V

M

P

P

N

P

E

G

L

A

Q
|
Q

V
x
I

G
|
G

Q

Q

T
|
T

G

G

K

K

I

I

I

V

A

A

P

P

E

T

L

V

Y

Y

M

F

A

A

F

V

G
|
G

I
x
V

S
|
S

G

G

A
|
A

I

S

Q
|
Q

H
|
H

L

L

T

A

G

G

I

I

K

S

D

N

A

A

G

K

T

C

I

V

V

I

A

A

I

I

N
|
N

K
|
K

D

D

G

N

E

E

A

A

P

N

I

I

F

F

E

K

I

R

A

A

D

R

I

F

G

G

L

I

V

V

G

G

D
|
D

L
x
Y

F

K

K

K

L

A

I

V

P

P

E

A

I

L

E

I

Q

N

A

A

L

L

binding flavin-adenine dinucleotide: G183 (= G205), R184 (= R206), G185 (= G207), S209 (= S231), R210 (= R232), Q223 (= Q245), I224 (≠ V246), G225 (= G247), T227 (= T249), G240 (= G262), V241 (≠ I263), S242 (= S264), A244 (= A266), Q246 (= Q268), H247 (= H269), N261 (= N283), K262 (= K284), D279 (= D301), Y280 (≠ L302)

7koeB Electron bifurcating flavoprotein fix/etfabcx (see paper)
34% identity, 97% coverage: 4:307/314 of query aligns to 7:321/336 of 7koeB

query
sites
7koeB

I

I

L

F

V

V

V

L

A

I

E

E

H

H

L

H

N

G

G

G

K

K

L

A

N

H

S

P

S

V

T

S

-

W

-

E

-

L

-

I

A

G

R

K

A

A

V

R

S

D

A

L

A

A

A

S

A

K

V

L

K

E

G

N

D

S

S

E

I

V

D

W

V

G

L

V

V

L

L

L

A

G

D

E

S

G

V

L

D

E

A

S

I

V

A

A

A

K

E

E

A

A

A

I

K

Q

I

-

D

R

G

G

V

A

G

D

K

K

V

V

L

L

T

Y

I

V

A

K

R

N

A

R

E

E

N

F

A

N

H

T

P

Y

L

V

A

N

A

Y

V

L

L

Y

A

K

P

K

Q

A

V

L

A

V

K

D

A

M

A

V

A

R

G

K

Y

Y

S

R

-

P

H

E

V

I

F

F

F

L

P

I

S

G

T

A

T

T

F

L

-

E

G

G

K

R

D

E

V

L

A

A

P

G

R

M

V

V

A

A

A

T

L

E

L

L

G

E

V

T

S

G

Q

L

V
x
T

S

A

D

D

V

C

M

T

S

G

V

L

E

D

-

I

-

P

-

D

-

K

G

K

A

L

H

L

S

A

F

M

K

T

R
|
R

P

P

I
x
T

Y

F

A

G

G

G

N

N

A

L

I

M

V

A

T

T

V

I

E

M

A

C

N

P

P

D

A

H

H

R

A

P

V

Q

V

M

A

A

T

T

I

V

R

R

-

P

-

G

-

V

T

M

A

K

S

E

W

L

P

P

A

P

A

D

G

P

S

E

R

R

G

T

D

G

S

-

A

-

V

-

E

E

A

I

L

I

A

E

V

E

D

E

A

Y

A

D

L

L

P

G

A

T

H

F

T

D

R

K

F

L

V

I

E

E

L

I

Q

L

Q

E

G

T

-

I

-

P

K

L

S

Q

D

T

R

Q

P

V

D

N

L

L

Q

E

G

Y

A

A

G

P

K

V

V

V

S

A

G

G

G
|
G

R
x
K

G
|
G

V

V

G

G

S

G

K

P

E

E

N

G

F

F

E

K

V

K

I

L

Y

K

K

E

L

L

A

A

D

D

K

L

I

L

G

G

A

G

A

E

V

V

G

G

A

A

S
|
S

R
|
R

A
|
A

A

A

V

V

D

K

A

A

G

G

Y

W

V

I

P

S

N

P

E

E

L

H

Q
|
Q

V
|
V

G
|
G

Q
|
Q

T
|
T

G

G

K

K

I

T

I

V

A

R

P

P

E

V

L

L

Y

Y

M

F

A

A

F

C

G
|
G

I

I

S
|
S

G

G

A

A

I

I

Q
|
Q

H
|
H

L

V

T

V

G

G

I

I

K

K

D

E

A

S

G

E

T

I

I

I

V

V

A

A

I

I

N
|
N

K
x
I

D

D

G

E

E

K

A

A

P

P

I

I

F

F

E

D

I

I

A

A

D

D

I

I

G

G

L

I

V

V

G

G

D

D

L
|
L

F

H

K

K

L

V

I

V

P

P

binding flavin-adenine dinucleotide: T121 (≠ V113), R140 (= R127), T142 (≠ I129), G219 (= G205), K220 (≠ R206), G221 (= G207), S245 (= S231), R246 (= R232), A247 (= A233), Q259 (= Q245), V260 (= V246), G261 (= G247), Q262 (= Q248), T263 (= T249), G276 (= G262), S278 (= S264), Q282 (= Q268), H283 (= H269), N297 (= N283), I298 (≠ K284), L316 (= L302)

P53571 Electron transfer flavoprotein subunit alpha; Alpha-ETF; Electron transfer flavoprotein large subunit; ETFLS from Methylophilus methylotrophus (Bacterium W3A1) (see 2 papers)
32% identity, 100% coverage: 1:313/314 of query aligns to 1:319/321 of P53571

query
sites
P53571

M
|
M

S

S

K

K

I

I

L

L

V

V

V

I

A

A

E

E

H

H

L

R

N

R

G

N

K

D

L

L

N

R

S

P

S

V

T

S

A

L

R

E

A

L

V

I

S

G

A

A

A

N

A

G

V

L

K

K

G

K

-

S

-

G

-

E

D

D

S

K

I

V

D

V

V

V

L

A

V

V

L

I

A

G

D

S

S

Q

V

A

D

D

A

A

I

F

A

V

A

P

E

-

A

A

A

L

K

S

I

V

D

N

G

G

V

V

G

D

K

E

V

L

L

V

T

V

I

V

A

K

R

G

A

S

E

S

-

I

N

D

A

F

H

D

P

P

L

D

A

V

A

F

V

E

L

A

A

S

P

V

Q

S

V

A

A

L

K

I

A

A

A

H

G

N

Y

P

S

S

H

V

V

V

F

L

P

P

S

H

T

S

T

V

F

D

G

S

K

L

D

G

V

Y

A

A

P

S

R

S

V

L

A

A

A

S

L

K

L

T

G

G

V

Y

S

G

Q

F

V

A

S

T

D

D

V

V

M

Y

S

I

V

V

E

E

G

Y

A

Q

H

G

S

D

F

E

K

L

R

V

P

A

I

T

Y

R

A

G

G

G

-

Y

N

N

A

Q

I

K

V

V

T

N

V

V

E

E

A

V

N

D

-

F

P

P

A

G

H

K

A

S

-

T

V

V

A

L

T

T

I

I

R

R

T

P

A

S

S

V

W

F

-

K

P

P

A

L

A

E

G

G

-

A

-

G

-

S

-

P

-

V

S

S

R

N

G

V

D

D

S

A

A

P

A

S

V

V

E

Q

A

S

L

R

A

S

V

-

D

-

A

-

L

-

P

-

A

Q

H

N

T

K

R

D

F

Y

V

V

E

E

L

V

Q

-

G

G

K

G

S

G

D

N

R

D

P

I

D

D

L

I

Q

T

G

T

A

V

G

D

K

F

V

I

V

M

S

S

G

I

G

G

R
|
R

G

G

V

I

G

G

S

E

K

E

E

T

N

N

F

V

E

E

V

Q

I

F

Y

R

K

E

L

L

A

A

D

D

K

E

I

A

G

G

A

A

A

T

V

L

G

C

A

C

S
|
S

R
|
R

A

P

A

I

V

A

D

D

A

A

G

G

Y

W

V

L

P

P

N

K

E

S

L

R

Q
|
Q

V
|
V

G
|
G

Q
|
Q

T
x
S

G

G

K

K

I

V

-

G

A

S

P

C

E

K

L

L

Y

Y

M

V

A

A

F

M

G
|
G

I
|
I

S
|
S

G
|
G

A
x
S

I
|
I

Q
|
Q

H
|
H

L

M

T

A

G

G

I

M

K

K

D

H

A

V

G

P

T

T

I

I

V

I

A

A

I

V

N
|
N

K

T

D

D

G

P

E

G

A

A

P

S

I

I

F

F

E

T

I

I

A

A

D

K

I

Y

G

G

L

I

V

V

G

A

D
|
D

L
x
I

F

F

K

D

L

I

I

E

P

E

E

E

I

L

E

K

Q

A

A

Q

L

L

M1 (= M1) modified: Initiator methionine, Removed
R211 (= R206) binding
SR 236:237 (= SR 231:232) binding
QVGQS 250:254 (≠ QVGQT 245:249) binding
268:275 (vs. 262:269, 88% identical) binding
N289 (= N283) binding
DI 307:308 (≠ DL 301:302) binding

3clrD Crystal structure of the r236a etf mutant from m. Methylotrophus (see paper)
32% identity, 99% coverage: 2:313/314 of query aligns to 1:318/319 of 3clrD

query
sites
3clrD

S

S

K

K

I

I

L

L

V

V

V

I

A

A

E

E

H

H

L

R

N

R

G

N

K

D

L

L

N

R

S

P

S

V

T

S

A

L

R

E

A

L

V

I

S

G

A

A

A

N

A

G

V

L

K

K

G

K

-

S

-

G

-

E

D

D

S

K

I

V

D

V

V

V

L

A

V

V

L

I

A

G

D

S

S

Q

V

A

D

D

A

A

I

F

A

V

A

P

E

-

A

A

A

L

K

S

I

V

D

N

G

G

V

V

G

D

K

E

V

L

L

V

T

V

I

V

A

K

R

G

A

S

E

S

-

I

N

D

A

F

H

D

P

P

L

D

A

V

A

F

V

E

L

A

A

S

P

V

Q

S

V

A

A

L

K

I

A

A

A

H

G

N

Y

P

S

S

H

V

V

V

F

L

P

P

S

H

T

S

T

V

F

D

G

S

K

L

D

G

V

Y

A

A

P

S

R

S

V

L

A

A

A

S

L

K

L

T

G

G

V

Y

S

G

Q

F

V

A

S

T

D

D

V

V

M

Y

S

I

V

V

E

E

G

Y

A

Q

H

G

S

D

F

E

K

L

R

V

P

A

I

T

Y

R

A

G

G

G

-

Y

N

N

A

Q

I

K

V

V

T

N

V

V

E

E

A

V

N

D

-

F

P

P

A

G

H

K

A

S

-

T

V

V

A

L

T

T

I

I

R

R

T

P

A

S

S

V

W

F

-

K

P

P

A

L

A

E

G

G

-

A

-

G

-

S

-

P

-

V

S

S

R

N

G

V

D

D

S

A

A

P

A

S

V

V

E

Q

A

S

L

R

A

S

V

-

D

-

A

-

L

-

P

-

A

Q

H

N

T

K

R

D

F

Y

V

V

E

E

L

V

Q

-

G

G

K

G

S

G

D

N

R

D

P

I

D

D

L

I

Q

T

G

T

A

V

G

D

K

F

V

I

V

M

S

S

G

I

G
|
G

R
|
R

G
|
G

V

I

G

G

S

E

K

E

E

T

N

N

F

V

E

E

V

Q

I

F

Y

R

K

E

L

L

A

A

D

D

K

E

I

A

G

G

A

A

A

T

V

L

G

C

A

C

S
|
S

R
x
A

A
x
P

A

I

V

A

D

D

A

A

G

G

Y

W

V

L

P

P

N

K

E

S

L

R

Q
|
Q

V
|
V

G
|
G

Q
|
Q

T
x
S

G

G

K

K

I

V

-

G

A

S

P

C

E

K

L

L

Y

Y

M

V

A

A

F

M

G
|
G

I
|
I

S
|
S

G

G

A
x
S

I

I

Q
|
Q

H
|
H

L

M

T

A

G

G

I

M

K

K

D

H

A

V

G

P

T

T

I

I

V

I

A

A

I

V

N
|
N

K
x
T

D

D

G

P

E

G

A

A

P

S

I

I

F

F

E

T

I

I

A

A

D

K

I

Y

G

G

L

I

V

V

G

A

D
|
D

L
x
I

F

F

K

D

L

I

I

E

P

E

E

E

I

L

E

K

Q

A

A

Q

L

L

binding flavin-adenine dinucleotide: G209 (= G205), R210 (= R206), G211 (= G207), S235 (= S231), A236 (≠ R232), P237 (≠ A233), Q249 (= Q245), V250 (= V246), G251 (= G247), Q252 (= Q248), S253 (≠ T249), G267 (= G262), I268 (= I263), S269 (= S264), S271 (≠ A266), Q273 (= Q268), H274 (= H269), N288 (= N283), T289 (≠ K284), D306 (= D301), I307 (≠ L302)

Query Sequence

>N515DRAFT_4319 N515DRAFT_4319 electron transfer flavoprotein alpha subunit apoprotein
MSKILVVAEHLNGKLNSSTARAVSAAAAVKGDSIDVLVLADSVDAIAAEAAKIDGVGKVL
TIARAENAHPLAAVLAPQVAKAAAGYSHVFFPSTTFGKDVAPRVAALLGVSQVSDVMSVE
GAHSFKRPIYAGNAIVTVEANPAHAVVATIRTASWPAAGSRGDSAAVEALAVDAALPAHT
RFVELQQGKSDRPDLQGAGKVVSGGRGVGSKENFEVIYKLADKIGAAVGASRAAVDAGYV
PNELQVGQTGKIIAPELYMAFGISGAIQHLTGIKDAGTIVAINKDGEAPIFEIADIGLVG
DLFKLIPEIEQALG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory