SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing N515DRAFT_4363 FitnessBrowser__Dyella79:N515DRAFT_4363 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6ld9A Crystal structure of cystathionine gamma synthase from xanthomonas oryzae pv. Oryzae in complex with cystathionine
67% identity, 96% coverage: 7:391/399 of query aligns to 2:387/387 of 6ld9A

query
sites
6ld9A

P

P

C

C

S

T

T

A

S

A

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A

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R

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N

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-

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Y

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binding (2~{S})-4-[(2~{R})-2-azanyl-3-oxidanyl-3-oxidanylidene-propyl]sulfanyl-2-[(~{E})-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]butanoic acid: Y46 (= Y51), R48 (= R53), S49 (= S54), G76 (= G81), M77 (= M82), Y101 (= Y105), R106 (= R110), N148 (= N152), D173 (= D177), S195 (= S199), T197 (= T201), K198 (= K202), N227 (= N231), E325 (= E329), S326 (= S330), T341 (= T345), R361 (= R365)

6lgoA Crystal structure of cystathionine gamma synthase from xanthomonas oryzae pv. Oryzae in complex with homolanthionine
67% identity, 96% coverage: 7:391/399 of query aligns to 2:387/387 of 6lgoA

query
sites
6lgoA

P

P

C

C

S

T

T

A

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T

T

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A

A

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V

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R

A

A

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G

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I

E

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D

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A

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Y

G

G

A

A

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V

V

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P

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S

T

S

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Y

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F

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D

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K

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Y

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G

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N

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D

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G

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-

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binding (2~{S})-2-azanyl-4-[(3~{S})-3-[(~{E})-[2-methyl-3-oxidanyl-5-(phosphonomethyl)pyridin-4-yl]methylideneamino]-4-oxidanyl-4-oxidanylidene-butyl]sulfanyl-butanoic acid: D45 (= D50), Y46 (= Y51), R48 (= R53), S49 (= S54), G76 (= G81), M77 (= M82), Y101 (= Y105), R106 (= R110), N148 (= N152), D173 (= D177), S195 (= S199), K198 (= K202), E325 (= E329), S326 (= S330), T341 (= T345), R361 (= R365)

6ld8A Crystal structure of cystathionine gamma synthase from xanthomonas oryzae pv. Oryzae in complex with aminoacrylate and cysteine
67% identity, 96% coverage: 7:391/399 of query aligns to 2:387/388 of 6ld8A

query
sites
6ld8A

P

P

C

C

S

T

T

A

S

A

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T

C

A

A

A

V

V

R

R

A

A

G

G

I

I

E

D

S

R

D

D

T

T

Q

A

H

Y

G

G

A

A

V

V

V

T

P

P

P

P

L

I

H

V

L

L

S

S

T

S

N

N

Y

F

A

S

F

F

E

D

G

G

L

F

G

G

G

N

K

K

R

R

A

Q

Y

Y

D

D

Y
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Y

S

T

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R

S
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S

G

G

N

N

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P

T

T

R

R

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D

L

L

L

L

G

G

N

E

A

A

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L

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E

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G

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A

A

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G

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G

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M

A

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A

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A

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L

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L

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L

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P

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L

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-

P

H

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C

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Y

G

G

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G

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W

W

R
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R

L

L

L

F

D

N

A

A

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L

A

A

K

K

K

K

G

G

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H

F

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I

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A

A

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D

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E

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K

A

K

A

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G

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N

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L

binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: Y46 (= Y51), R48 (= R53), G76 (= G81), M77 (= M82), Y101 (= Y105), N148 (= N152), D173 (= D177), S195 (= S199), T197 (= T201), K198 (= K202), S326 (= S330), T341 (= T345), R361 (= R365)
binding cysteine: Y46 (= Y51), R48 (= R53), S49 (= S54), Y101 (= Y105), R106 (= R110), E325 (= E329), T341 (= T345)

P00935 Cystathionine gamma-synthase; CGS; O-succinylhomoserine (thiol)-lyase; EC 2.5.1.48 from Escherichia coli (strain K12) (see paper)
57% identity, 94% coverage: 11:384/399 of query aligns to 6:380/386 of P00935

query
sites
P00935

S

A

T

T

C

I

A

A

V

V

R

R

A

S

G

G

I

L

E

N

S

D

D

D

T

E

Q

Q

H

Y

G

G

A

C

V

V

V

V

P

P

P

P

L

I

H

H

L

L

S

S

T

S

N

T

Y

Y

A

N

F

F

E

T

G

G

L

F

G

N

G

E

K

P

R

R

A

A

Y

H

D

D

Y

Y

S

S

R

R

S

R

G

G

N

N

P

P

T

T

R

R

D

D

L

V

L

V

G

Q

N

R

A

A

L

L

A

A

E

E

L

L

E

E

Q

G

G

G

A

A

G

G

A

A

V

V

V

L

T

T

A

N

S

T

G

G

M

M

A

S

A

A

V

I

A

H

L

L

A

V

L

T

E

T

L

V

V

F

P

L

A

K

G

P

G

G

R

D

V

L

L

L

A

V

A

A

-

P

H

H

D

D

C

C

Y

Y

G

G

G

G

T

S

W

Y

R

R

L

L

L

F

D

D

A

S

W

L

A

A

K

K

K

R

G

G

R

C

F

Y

T

R

L

V

E

L

F

F

A

V

D

D

L

Q

T

G

D

D

R

E

V

Q

A

A

L

L

A

R

A

A

G

A

L

L

A

A

S

E

K

K

P

P

S

K

L

L

V

V

W

L

V

V

E

E

T

S

P

P

S

S

N

N

P

P

L

L

L

L

R

R

I

V

T

V

D

D

V

I

R

A

H

K

V

I

A

C

Q

H

A

L

A

A

H

R

A

E

A

V

G

G

A

A

L

V

V

S

V

V

V

V

D

D

N

N

T

T

F

F

L

L

S

S

P

P

A

A

L

L

Q

Q

Q

N

P

P

L

L

V

A

L

L

G

G

A

A

D

D

V

L

V

V

V

L

H

H

S

S

T

C

T

T

K
|
K

Y

Y

I

L

N

N

G

G

H

H

S

S

D

D

V

V

V

V

G

A

G

G

A

V

V

V

V

I

A

A

R

K

D

D

Q

P

A

D

V

V

A

V

D

T

Q

E

L

L

K

A

W

W

W

W

G

A

N

N

C

N

N

I

G

G

L

V

T

T

G

G

A

G

P

A

F

F

D

D

S

S

F

Y

L

L

T

L

L

L

R

R

G

G

L

L

R

R

T

T

L

L

S

V

V

P

R

R

L

M

R

E

Q

L

H

A

Q

Q

E

R

N

N

A

A

Q

Q

R

A

I

I

A

V

T

K

L

Y

L

L

D

Q

G

T

H

Q

A

P

A

L

V

V

R

K

K

K

V

L

Y

Y

Y

H

P

P

G

S

L

L

E

P

S

E

H

N

P

Q

G

G

H

H

A

E

L

I

A

A

A

A

R

R

Q

Q

Q

Q

A

K

G

G

F

F

G

G

A

A

M

M

L

L

S

S

F

F

E

E

L

L

E

D

G

G

D

D

V

E

A

Q

Q

T

I

L

E

R

A

R

F

F

V

L

N

G

E

G

L

L

K

S

Y

L

F

F

S

T

L

L

A

A

E

E

S

S

L

L

G

G

G

G

V

V

E

E

S

S

L

L

V

I

A

S

H

H

P

A

A

A

S

T

M

M

T

T

H

H

A

A

S

G

M

M

A

A

P

P

E

E

A

A

R

R

R

A

T

A

A

A

G

G

I

I

A

S

D

E

S

T

L

L

L

L

R

R

I

I

S

S

V

T

G

G

I

I

E

E

D

D

G

G

D

E

D

D

L

L

V

I

R

A

D

D

V

L

D

E

A

N

G

G

L

F

K198 (= K202) modified: N6-(pyridoxal phosphate)lysine

1cs1D Cystathionine gamma-synthase (cgs) from escherichia coli (see paper)
57% identity, 94% coverage: 11:384/399 of query aligns to 4:378/384 of 1cs1D

query
sites
1cs1D

S

A

T

T

C

I

A

A

V

V

R

R

A

S

G

G

I

L

E

N

S

D

D

D

T

E

Q

Q

H

Y

G

G

A

C

V

V

V

V

P

P

P

P

L

I

H

H

L

L

S

S

T

S

N

T

Y

Y

A

N

F

F

E

T

G

G

L

F

G

N

G

E

K

P

R

R

A

A

Y

H

D

D

Y

Y

S

S

R
|
R

S

R

G

G

N

N

P

P

T

T

R

R

D

D

L

V

L

V

G

Q

N

R

A

A

L

L

A

A

E

E

L

L

E

E

Q

G

G

G

A

A

G

G

A

A

V

V

V

L

T

T

A

N

S

T

G

G

M

M

A

S

A

A

V

I

A

H

L

L

A

V

L

T

E

T

L

V

V

F

P

L

A

K

G

P

G

G

R

D

V

L

L

L

A

V

A

A

-

P

H

H

D

D

C

C

Y
|
Y

G

G

G

G

T

S

W

Y

R

R

L

L

L

F

D

D

A

S

W

L

A

A

K

K

K

R

G

G

R

C

F

Y

T

R

L

V

E

L

F

F

A

V

D

D

L

Q

T

G

D

D

R

E

V

Q

A

A

L

L

A

R

A

A

G

A

L

L

A

A

S

E

K

K

P

P

S

K

L

L

V

V

W

L

V

V

E

E

T

S

P

P

S

S

N

N

P

P

L

L

L

L

R

R

I

V

T

V

D

D

V

I

R
x
A

H
x
K

V

I

A

C

Q

H

A

L

A

A

H

R

A

E

A

V

G

G

A

A

L

V

V

S

V

V

V

V

D
|
D

N

N

T

T

F

F

L

L

S

S

P

P

A

A

L

L

Q

Q

Q

N

P

P

L

L

V

A

L

L

G

G

A

A

D

D

V

L

V

V

V

L

H

H

S

S

T

C

T

T

K
|
K

Y

Y

I

L

N

N

G

G

H

H

S

S

D

D

V

V

V

V

G

A

G

G

A

V

V

V

V

I

A

A

R

K

D

D

Q

P

A

D

V

V

A

V

D

T

Q

E

L

L

K

A

W

W

W

W

G

A

N

N

C

N

N

I

G

G

L

V

T

T

G

G

A

G

P

A

F

F

D

D

S

S

F

Y

L

L

T

L

L

L

R

R

G

G

L

L

R

R

T

T

L

L

S

V

V

P

R

R

L

M

R

E

Q

L

H

A

Q

Q

E

R

N

N

A

A

Q

Q

R

A

I

I

A

V

T

K

L

Y

L

L

D

Q

G

T

H

Q

A

P

A

L

V

V

R

K

K

K

V

L

Y

Y

Y

H

P

P

G

S

L

L

E

P

S

E

H

N

P

Q

G

G

H

H

A

E

L

I

A

A

A

A

R

R

Q

Q

Q

Q

A

K

G

G

F

F

G

G

A

A

M

M

L

L

S

S

F

F

E

E

L

L

E

D

G

G

D

D

V

E

A

Q

Q

T

I

L

E

R

A

R

F

F

V

L

N

G

E

G

L

L

K

S

Y

L

F

F

S

T

L

L

A

A

E

E

S

S

L

L

G

G

G

G

V

V

E

E

S

S

L

L

V

I

A

S

H

H

P

A

A

A

S

T

M

M

T

T

H

H

A

A

S

G

M

M

A

A

P

P

E

E

A

A

R

R

R

A

T

A

A

A

G

G

I

I

A

S

D

E

S

T

L

L

L

L

R

R

I

I

S

S

V

T

G

G

I

I

E

E

D

D

G

G

D

E

D

D

L

L

V

I

R

A

D

D

V

L

D

E

A

N

G

G

L

F

active site: R46 (= R53), Y99 (= Y105), D171 (= D177), K196 (= K202)
binding 2,4-dioxo-pentanedioic acid: A155 (≠ R161), K156 (≠ H162)

4iyoD Crystal structure of cystathionine gamma lyase from xanthomonas oryzae pv. Oryzae (xometc) in complex with e-site serine, a-site serine, a- site external aldimine structure with aminoacrylate and a-site iminopropionate intermediates (see paper)
48% identity, 94% coverage: 9:384/399 of query aligns to 3:380/384 of 4iyoD

query
sites
4iyoD

S

S

T

L

S

A

T

T

C

L

A

A

V

I

R

H

A

G

G

G

I

Q

E

S

S

P

D

D

T

P

Q

S

H

T

G

G

A

A

V

V

V

M

P

P

P

P

L

I

H

Y

L

A

S

T

T

S

N

T

Y

Y

A

A

F

Q

E

S

G

S

L

P

G

G

G

E

K

H

R

Q

A

G

Y

F

D

E

Y
|
Y

S

S

R
|
R

S
x
T

G

H

N

N

P

P

T

T

R

R

D

F

L

A

L

Y

G

E

N

R

A

C

L

V

A

A

E

A

L

L

E

E

Q

G

G

G

A

T

G

R

A

A

V

F

V

A

T

F

A

A

S

S

G

G

M

M

A

A

A

A

V

T

A

S

L

T

A

V

L

M

E

E

L

L

V

L

P

D

A

A

G

G

G

S

R

H

V

V

L

V

A

A

A

M

H

D

D

D

C

L

Y
|
Y

G

G

G

G

T

T

W

F

R
|
R

L

L

L

F

D

E

A

R

W

V

A

R

K

R

K

R

-

T

G

A

R

G

F

L

T

D

L

F

E

S

F

F

A

V

D

D

L

L

T

T

D

D

R

P

V

A

A

A

L

F

A

K

A

A

G

A

L

I

A

R

S

A

K

D

P

T

S

K

L

M

V

V

W

W

V

I

E

E

T

T

P

P

S

T

N

N

P

P

L

M

L

L

R

K

I

L

T

V

D

D

V

I

R

A

H

A

V

I

A

A

Q

V

A

I

A

A

H

R

A

K

A

H

G

G

A

L

L

L

V

T

V

V

V

V

D
|
D

N

N

T

T

F

F

L

A

S

S

P

P

A

M

L

L

Q

Q

Q

R

P

P

L

L

V

S

L

L

G

G

A

A

D

D

V

L

V

V

V

V

H

H

S

S

T

A

T

T

K
|
K

Y

Y

I

L

N

N

G

G

H

H

S

S

D

D

V

M

V

V

G

G

G

G

A

I

V

A

V

V

A

V

R

G

D

D

Q

N

A

A

-

E

V

L

A

A

D

E

Q

Q

L

M

K

A

W

F

W

L

G

Q

N
|
N

C

S

N

I

G

G

L

G

T

V

G

Q

A

G

P

P

F

F

D

D

S

S

F

F

L

L

T

A

L

L

R

R

G

G

L

L

R

K

T

T

L

L

S

P

V

L

R

R

L

M

R

R

Q

A

H

H

Q

C

E

E

N

N

A

A

Q

L

R

A

I

L

A

A

T

Q

L

W

L

L

D

E

G

T

H

H

A

P

A

A

V

I

R

E

K

K

V

V

Y

I

Y

Y

P

P

G

G

L

L

E

A

S

S

H

H

P

P

G

Q

H

H

A

V

L

L

A

A

A

K

R

R

Q

Q

Q

M

A

S

G

G

F

F

G

G

A

G

M

I

L

V

S

S

F

I

E

V

L

L

E

K

G

G

D

G

V

F

A

D

Q

A

I

A

E

K

A

R

F

F

V

C

N

E

E

K

L

T

K

E

Y

L

F

F

S

T

L

L

A

A

E
|
E

S
|
S

L

L

G

G

G

G

V

V

E

E

S

S

L

L

V

V

A

N

H

H

P

P

A

A

S

V

M

M

T
|
T

H

H

A

A

S

S

M

I

A

P

P

V

E

A

A

R

R

R

R

E

T

Q

A

L

G

G

I

I

A

S

D

D

S

A

L

L

L

V

R
|
R

I

L

S

S

V

V

G

G

I

I

E

E

D

D

G

L

D

G

D

D

L

L

V

R

R

G

D

D

V

L

D

E

A

R

G

A

L

L

active site: R47 (= R53), Y99 (= Y105), D172 (= D177), K197 (= K202)
binding serine: Y45 (= Y51), T48 (≠ S54), Y99 (= Y105), Y99 (= Y105), R104 (= R110), K197 (= K202), N227 (= N231), E325 (= E329), S326 (= S330), T341 (= T345), R361 (= R365)

4iyoB Crystal structure of cystathionine gamma lyase from xanthomonas oryzae pv. Oryzae (xometc) in complex with e-site serine, a-site serine, a- site external aldimine structure with aminoacrylate and a-site iminopropionate intermediates (see paper)
48% identity, 94% coverage: 9:384/399 of query aligns to 3:380/381 of 4iyoB

query
sites
4iyoB

S

S

T

L

S

A

T

T

C

L

A

A

V

I

R

H

A

G

G

G

I

Q

E

S

S

P

D

D

T

P

Q

S

H

T

G

G

A

A

V

V

V

M

P

P

P

P

L

I

H

Y

L

A

S

T

T

S

N

T

Y

Y

A

A

F

Q

E

S

G

S

L

P

G

G

G

E

K

H

R

Q

A

G

Y

F

D

E

Y
|
Y

S

S

R
|
R

S
x
T

G

H

N

N

P

P

T

T

R

R

D

F

L

A

L

Y

G

E

N

R

A

C

L

V

A

A

E

A

L

L

E

E

Q

G

G

G

A

T

G

R

A

A

V

F

V

A

T

F

A

A

S

S

G

G

M

M

A

A

A

A

V

T

A

S

L

T

A

V

L

M

E

E

L

L

V

L

P

D

A

A

G

G

G

S

R

H

V

V

L

V

A

A

A

M

H

D

D

D

C

L

Y
|
Y

G

G

G

G

T

T

W

F

R
|
R

L

L

L

F

D

E

A

R

W

V

A

R

K

R

K

R

-

T

G

A

R

G

F

L

T

D

L

F

E

S

F

F

A

V

D

D

L

L

T

T

D

D

R

P

V

A

A

A

L

F

A

K

A

A

G

A

L

I

A

R

S

A

K

D

P

T

S

K

L

M

V

V

W

W

V

I

E

E

T

T

P

P

S

T

N

N

P

P

L

M

L

L

R

K

I

L

T

V

D

D

V

I

R

A

H

A

V

I

A

A

Q

V

A

I

A

A

H

R

A

K

A

H

G

G

A

L

L

L

V

T

V

V

V

V

D
|
D

N

N

T

T

F

F

L

A

S

S

P

P

A

M

L

L

Q

Q

Q

R

P

P

L

L

V

S

L

L

G

G

A

A

D

D

V

L

V

V

V

V

H

H

S

S

T

A

T

T

K
|
K

Y

Y

I

L

N

N

G

G

H

H

S

S

D

D

V

M

V

V

G

G

G

G

A

I

V

A

V

V

A

V

R

G

D

D

Q

N

A

A

-

E

V

L

A

A

D

E

Q
|
Q

L

M

K

A

W
x
F

W

L

G

Q

N
|
N

C

S

N

I

G

G

L

G

T

V

G

Q

A

G

P

P

F

F

D

D

S

S

F

F

L

L

T

A

L

L

R

R

G

G

L

L

R

K

T

T

L

L

S

P

V

L

R

R

L

M

R

R

Q

A

H

H

Q

C

E

E

N

N

A

A

Q

L

R

A

I

L

A

A

T

Q

L

W

L

L

D

E

G

T

H

H

A

P

A

A

V

I

R

E

K

K

V

V

Y

I

Y

Y

P

P

G

G

L

L

E

A

S

S

H

H

P

P

G

Q

H

H

A

V

L

L

A

A

A

K

R

R

Q

Q

Q

M

A

S

G

G

F

F

G

G

A

G

M

I

L

V

S

S

F

I

E

V

L

L

E

K

G

G

D

G

V

F

A

D

Q

A

I

A

E

K

A

R

F

F

V

C

N

E

E

K

L

T

K

E

Y

L

F

F

S

T

L

L

A

A

E
|
E

S
|
S

L

L

G

G

G

G

V

V

E

E

S

S

L

L

V

V

A

N

H

H

P

P

A

A

S

V

M

M

T
|
T

H

H

A

A

S

S

M

I

A

P

P

V

E

A

A

R

R

R

R

E

T

Q

A

L

G

G

I

I

A

S

D

D

S

A

L

L

L

V

R
|
R

I

L

S

S

V

V

G

G

I

I

E

E

D

D

G

L

D

G

D

D

L

L

V

R

R

G

D

D

V

L

D

E

A

R

G

A

L

L

active site: R47 (= R53), Y99 (= Y105), D172 (= D177), K197 (= K202)
binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: Y45 (= Y51), R47 (= R53)
binding amino-acrylate: Y99 (= Y105), K197 (= K202), S326 (= S330), T341 (= T345), R361 (= R365)
binding pyruvic acid: Q221 (= Q225), F224 (≠ W228)
binding serine: Y45 (= Y51), T48 (≠ S54), Y99 (= Y105), R104 (= R110), N227 (= N231), E325 (= E329)

4iy7B Crystal structure of cystathionine gamma lyase (xometc) from xanthomonas oryzae pv. Oryzae in complex with e-site serine, a-site external aldimine structure with serine and a-site external aldimine structure with aminoacrylate intermediates (see paper)
48% identity, 94% coverage: 9:384/399 of query aligns to 3:380/381 of 4iy7B

query
sites
4iy7B

S

S

T

L

S

A

T

T

C

L

A

A

V

I

R

H

A

G

G

G

I

Q

E

S

S

P

D

D

T

P

Q

S

H

T

G

G

A

A

V

V

V

M

P

P

P

P

L

I

H

Y

L

A

S

T

T

S

N

T

Y

Y

A

A

F

Q

E

S

G

S

L

P

G

G

G

E

K

H

R

Q

A

G

Y

F

D

E

Y
|
Y

S

S

R
|
R

S
x
T

G

H

N

N

P

P

T

T

R

R

D

F

L

A

L

Y

G

E

N

R

A

C

L

V

A

A

E

A

L

L

E

E

Q

G

G

G

A

T

G

R

A

A

V

F

V

A

T

F

A

A

S

S

G
|
G

M
|
M

A

A

A

A

V

T

A

S

L

T

A

V

L

M

E

E

L

L

V

L

P

D

A

A

G

G

G

S

R

H

V

V

L

V

A

A

A

M

H

D

D

D

C

L

Y
|
Y

G

G

G

G

T

T

W

F

R
|
R

L

L

L

F

D

E

A

R

W

V

A

R

K

R

K

R

-

T

G

A

R

G

F

L

T

D

L

F

E

S

F

F

A

V

D

D

L

L

T

T

D

D

R

P

V

A

A

A

L

F

A

K

A

A

G

A

L

I

A

R

S

A

K

D

P

T

S

K

L

M

V

V

W

W

V

I

E
|
E

T

T

P

P

S

T

N
|
N

P

P

L

M

L

L

R

K

I

L

T

V

D

D

V

I

R

A

H

A

V

I

A

A

Q

V

A

I

A

A

H

R

A

K

A

H

G

G

A

L

L

L

V

T

V

V

V

V

D
|
D

N

N

T

T

F

F

L

A

S

S

P

P

A

M

L

L

Q

Q

Q

R

P

P

L

L

V

S

L

L

G

G

A

A

D

D

V

L

V

V

V

V

H

H

S
|
S

T

A

T

T

K
|
K

Y

Y

I

L

N

N

G

G

H

H

S

S

D

D

V

M

V

V

G

G

G

G

A

I

V

A

V

V

A

V

R

G

D

D

Q

N

A

A

-

E

V

L

A

A

D

E

Q
|
Q

L

M

K

A

W
x
F

W

L

G

Q

N
|
N

C

S

N

I

G

G

L

G

T

V

G

Q

A

G

P

P

F

F

D

D

S

S

F

F

L

L

T

A

L

L

R

R

G

G

L

L

R

K

T

T

L

L

S

P

V

L

R

R

L

M

R

R

Q

A

H

H

Q

C

E

E

N

N

A

A

Q

L

R

A

I

L

A

A

T

Q

L

W

L

L

D

E

G

T

H

H

A

P

A

A

V

I

R

E

K

K

V

V

Y

I

Y

Y

P

P

G

G

L

L

E

A

S

S

H

H

P

P

G

Q

H

H

A

V

L

L

A

A

A

K

R

R

Q

Q

Q

M

A

S

G

G

F

F

G

G

A

G

M

I

L

V

S

S

F

I

E

V

L

L

E

K

G

G

D

G

V

F

A

D

Q

A

I

A

E

K

A

R

F

F

V

C

N

E

E

K

L

T

K

E

Y

L

F

F

S

T

L

L

A

A

E
|
E

S
|
S

L
|
L

G

G

G

G

V

V

E

E

S

S

L

L

V

V

A

N

H

H

P

P

A

A

S

V

M

M

T
|
T

H

H

A

A

S

S

M

I

A

P

P

V

E

A

A

R

R

R

R

E

T

Q

A

L

G

G

I

I

A

S

D

D

S

A

L

L

L

V

R
|
R

I

L

S

S

V

V

G

G

I

I

E

E

D

D

G

L

D

G

D

D

L

L

V

R

R

G

D

D

V

L

D

E

A

R

G

A

L

L

active site: R47 (= R53), Y99 (= Y105), D172 (= D177), K197 (= K202)
binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: Y45 (= Y51), R47 (= R53)
binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: G75 (= G81), M76 (= M82), Y99 (= Y105), E143 (= E148), N147 (= N152), D172 (= D177), S194 (= S199), K197 (= K202), S326 (= S330), L327 (= L331), T341 (= T345), R361 (= R365)
binding pyruvic acid: Q221 (= Q225), F224 (≠ W228)
binding serine: Y45 (= Y51), T48 (≠ S54), Y99 (= Y105), R104 (= R110), N227 (= N231), E325 (= E329)

4iy7A Crystal structure of cystathionine gamma lyase (xometc) from xanthomonas oryzae pv. Oryzae in complex with e-site serine, a-site external aldimine structure with serine and a-site external aldimine structure with aminoacrylate intermediates (see paper)
48% identity, 94% coverage: 9:384/399 of query aligns to 3:380/381 of 4iy7A

query
sites
4iy7A

S

S

T

L

S

A

T

T

C

L

A

A

V

I

R

H

A

G

G

G

I

Q

E

S

S

P

D

D

T

P

Q

S

H

T

G

G

A

A

V

V

V

M

P

P

P

P

L

I

H

Y

L

A

S

T

T

S

N

T

Y

Y

A

A

F

Q

E

S

G

S

L

P

G

G

G

E

K

H

R

Q

A

G

Y

F

D

E

Y
|
Y

S

S

R
|
R

S
x
T

G

H

N

N

P

P

T

T

R

R

D

F

L

A

L

Y

G

E

N

R

A

C

L

V

A

A

E

A

L

L

E

E

Q

G

G

G

A

T

G

R

A

A

V

F

V

A

T

F

A

A

S

S

G
|
G

M
|
M

A

A

A

A

V

T

A

S

L

T

A

V

L

M

E

E

L

L

V

L

P

D

A

A

G

G

G

S

R

H

V

V

L

V

A

A

A

M

H

D

D

D

C

L

Y
|
Y

G

G

G

G

T

T

W

F

R
|
R

L

L

L

F

D

E

A

R

W

V

A

R

K

R

K

R

-

T

G

A

R

G

F

L

T

D

L

F

E

S

F

F

A

V

D

D

L

L

T

T

D

D

R

P

V

A

A

A

L

F

A

K

A

A

G

A

L

I

A

R

S

A

K

D

P

T

S

K

L

M

V

V

W

W

V

I

E
|
E

T

T

P

P

S

T

N
|
N

P

P

L

M

L

L

R

K

I

L

T

V

D

D

V

I

R

A

H

A

V

I

A

A

Q

V

A

I

A

A

H

R

A

K

A

H

G

G

A

L

L

L

V

T

V

V

V

V

D
|
D

N

N

T

T

F

F

L

A

S

S

P

P

A

M

L

L

Q

Q

Q

R

P

P

L

L

V

S

L

L

G

G

A

A

D

D

V

L

V

V

V

V

H

H

S
|
S

T

A

T

T

K
|
K

Y

Y

I

L

N

N

G

G

H

H

S

S

D

D

V

M

V

V

G

G

G

G

A

I

V

A

V

V

A

V

R

G

D

D

Q

N

A

A

-

E

V

L

A

A

D

E

Q

Q

L

M

K

A

W

F

W

L

G

Q

N
|
N

C

S

N

I

G

G

L

G

T

V

G

Q

A

G

P

P

F

F

D

D

S

S

F

F

L

L

T

A

L

L

R

R

G

G

L

L

R

K

T

T

L

L

S

P

V

L

R

R

L

M

R

R

Q

A

H

H

Q

C

E

E

N

N

A

A

Q

L

R

A

I

L

A

A

T

Q

L

W

L

L

D

E

G

T

H

H

A

P

A

A

V

I

R

E

K

K

V

V

Y

I

Y

Y

P

P

G

G

L

L

E

A

S

S

H

H

P

P

G

Q

H

H

A

V

L

L

A

A

A

K

R

R

Q

Q

Q

M

A

S

G

G

F

F

G

G

A

G

M

I

L

V

S

S

F

I

E

V

L

L

E

K

G

G

D

G

V

F

A

D

Q

A

I

A

E

K

A

R

F

F

V

C

N

E

E

K

L

T

K

E

Y

L

F

F

S

T

L

L

A

A

E
|
E

S
|
S

L
|
L

G

G

G

G

V

V

E

E

S

S

L

L

V

V

A

N

H

H

P

P

A

A

S

V

M

M

T
|
T

H

H

A

A

S

S

M

I

A

P

P

V

E

A

A

R

R

R

R

E

T

Q

A

L

G

G

I

I

A

S

D

D

S

A

L

L

L

V

R
|
R

I

L

S

S

V

V

G

G

I

I

E

E

D

D

G

L

D

G

D

D

L

L

V

R

R

G

D

D

V

L

D

E

A

R

G

A

L

L

active site: R47 (= R53), Y99 (= Y105), D172 (= D177), K197 (= K202)
binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: G75 (= G81), M76 (= M82), Y99 (= Y105), E143 (= E148), N147 (= N152), D172 (= D177), S194 (= S199), K197 (= K202), S326 (= S330), L327 (= L331), T341 (= T345), R361 (= R365)
binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: Y45 (= Y51), R47 (= R53)
binding serine: Y45 (= Y51), T48 (≠ S54), Y99 (= Y105), R104 (= R110), N227 (= N231), E325 (= E329)

4ixzA Native structure of cystathionine gamma lyase (xometc) from xanthomonas oryzae pv. Oryzae at ph 9.0 (see paper)
48% identity, 94% coverage: 9:384/399 of query aligns to 3:380/381 of 4ixzA

query
sites
4ixzA

S

S

T

L

S

A

T

T

C

L

A

A

V

I

R

H

A

G

G

G

I

Q

E

S

S

P

D

D

T

P

Q

S

H

T

G

G

A

A

V

V

V

M

P

P

P

P

L

I

H

Y

L

A

S

T

T

S

N

T

Y

Y

A

A

F

Q

E

S

G

S

L

P

G

G

G

E

K

H

R

Q

A

G

Y

F

D

E

Y

Y

S

S

R
|
R

S

T

G

H

N

N

P

P

T

T

R

R

D

F

L

A

L

Y

G

E

N

R

A

C

L

V

A

A

E

A

L

L

E

E

Q

G

G

G

A

T

G

R

A

A

V

F

V

A

T

F

A

A

S

S

G

G

M

M

A

A

A

A

V

T

A

S

L

T

A

V

L

M

E

E

L

L

V

L

P

D

A

A

G

G

G

S

R

H

V

V

L

V

A

A

A

M

H

D

D

D

C

L

Y
|
Y

G

G

G

G

T

T

W

F

R

R

L

L

L

F

D

E

A

R

W

V

A

R

K

R

K

R

-

T

G

A

R

G

F

L

T

D

L

F

E

S

F

F

A

V

D

D

L

L

T

T

D

D

R

P

V

A

A

A

L

F

A

K

A

A

G

A

L

I

A

R

S

A

K

D

P

T

S

K

L

M

V

V

W

W

V

I

E

E

T

T

P

P

S

T

N

N

P

P

L

M

L

L

R

K

I

L

T

V

D

D

V

I

R

A

H

A

V

I

A

A

Q

V

A

I

A

A

H

R

A

K

A

H

G

G

A

L

L

L

V

T

V

V

V

V

D
|
D

N

N

T

T

F

F

L

A

S

S

P

P

A

M

L

L

Q

Q

Q

R

P

P

L

L

V

S

L

L

G

G

A

A

D

D

V

L

V

V

V

V

H

H

S

S

T

A

T

T

K
|
K

Y

Y

I

L

N

N

G

G

H

H

S

S

D

D

V

M

V

V

G

G

G

G

A

I

V

A

V

V

A

V

R

G

D

D

Q

N

A

A

-

E

V

L

A

A

D

E

Q

Q

L

M

K

A

W

F

W

L

G

Q

N

N

C

S

N

I

G

G

L

G

T

V

G

Q

A

G

P

P

F

F

D

D

S

S

F

F

L

L

T

A

L

L

R

R

G

G

L

L

R

K

T

T

L

L

S

P

V

L

R

R

L

M

R

R

Q

A

H

H

Q

C

E

E

N

N

A

A

Q

L

R

A

I

L

A

A

T

Q

L

W

L

L

D

E

G

T

H

H

A

P

A

A

V

I

R

E

K

K

V

V

Y

I

Y

Y

P

P

G

G

L

L

E

A

S

S

H

H

P

P

G

Q

H

H

A

V

L

L

A

A

A

K

R

R

Q

Q

Q

M

A

S

G

G

F

F

G

G

A

G

M

I

L

V

S

S

F

I

E

V

L

L

E

K

G

G

D

G

V

F

A

D

Q

A

I

A

E

K

A

R

F

F

V

C

N

E

E

K

L

T

K

E

Y

L

F

F

S

T

L

L

A

A

E

E

S
|
S

L
|
L

G

G

G

G

V

V

E

E

S

S

L

L

V

V

A

N

H

H

P

P

A

A

S

V

M

M

T
|
T

H

H

A

A

S

S

M

I

A

P

P

V

E

A

A

R

R

R

R

E

T

Q

A

L

G

G

I

I

A

S

D

D

S

A

L

L

L

V

R
|
R

I

L

S

S

V

V

G

G

I

I

E

E

D

D

G

L

D

G

D

D

L

L

V

R

R

G

D

D

V

L

D

E

A

R

G

A

L

L

active site: R47 (= R53), Y99 (= Y105), D172 (= D177), K197 (= K202)
binding bicarbonate ion: Y99 (= Y105), S326 (= S330), L327 (= L331), T341 (= T345), R361 (= R365)

7ba4A Structure of cystathionine gamma-lyase from pseudomonas aeruginosa
47% identity, 94% coverage: 11:387/399 of query aligns to 8:374/377 of 7ba4A

query
sites
7ba4A

S

A

T

T

C

R

A

V

V

I

R

H

A

A

G

G

I

Q

E

A

S

P

D

D

T

P

Q

S

H

T

G

G

A

A

V

I

V

M

P

P

P

P

L

I

H

Y

L

A

S

N

T

S

N

T

Y

Y

A

-

F

-

E

-

G

-

L

-

G

-

G

-

K

-

R

-

A

-

Y

-

D

-

Y

I

S

Q

R

R

S

S

G

H

N

N

P

P

T

T

R

R

D

W

L

A

L

L

G

E

N

R

A

C

L

V

A

A

E

D

L

L

E

E

Q

G

G

G

A

T

G

Q

A

A

V

F

V

A

T

F

A

A

S

S

G
|
G

M
x
L

A

A

A

A

V

I

A

S

L

S

A

V

L

L

E

E

L

L

V

L

P

D

A

A

G

G

G

S

R

H

V

I

L

V

A

S

A

G

H

N

D

D

C

L

Y
|
Y

G

G

G

G

T

T

W

F

R

R

L

L

L

F

D

E

A

R

W

V

A

R

K

R

K

R

G

S

-

A

-

G

-

H

R

R

F

F

T

S

L

-

E

-

F

F

A

V

D

D

L

P

T

T

D

D

R

L

V

Q

A

A

L

F

A

E

A

A

G

A

L

L

A

T

S

P

K

E

P

T

S

R

L

M

V

V

W

W

V

V

E

E

T

T

P

P

S

S

N

N

P

P

L

L

L

L

R

R

I

L

T

T

D

D

V

L

R

R

H

A

V

I

A

A

Q

Q

A

L

A

C

H

R

A

A

A

R

G

G

A

I

L

I

V

S

V

V

V

A

D
|
D

N

N

T

T

F
|
F

L

A

S

S

P

P

A

Y

L

I

Q

Q

Q

R

P

P

L

L

V

E

L

L

G

G

A

F

D

D

V

V

V

V

V

V

H

H

S
|
S

T

T

T
|
T

K
|
K

Y

Y

I

L

N

N

G

G

H

H

S

S

D

D

V

V

V

I

G

G

G

G

-

I

A

A

V

I

V

V

A

G

R

D

D

N

Q

P

A

D

V

L

A

R

D

E

Q

R

L

L

K

G

W

F

W

L

G

Q

N

N

C

S

N

V

G

G

L

A

T

I

G

S

A

G

P

P

F

F

D

D

S

A

F

F

L

L

T

T

L

L

R

R

G

G

L

V

R

K

T

T

L

L

S

A

V

L

R

R

L

M

R

E

Q

R

H

H

Q

C

E

S

N

N

A

A

Q

L

R

A

I

L

A

A

T

Q

L

W

L

L

D

E

G

R

H

Q

A

P

A

Q

V

V

R

A

K

R

V

V

Y

Y

Y

Y

P

P

G

G

L

L

E

A

S

S

H

H

P

P

G

Q

H

H

A

E

L

L

A

A

A

K

R

R

Q

Q

Q

M

A

R

G

G

F

F

G

G

A

G

M

M

L

I

S

S

F

L

E

D

L

L

E

R

G

C

D

D

V

L

A

A

Q

G

I

A

E

R

A

R

F

F

V

L

N

E

E

N

L

V

K

R

Y

I

F

F

S

S

L

L

A

A

E

E

S

S

L

L

G

G

G

G

V

V

E

E

S

S

L

L

V

I

A

E

H

H

P

P

A

A

S

I

M

M

T

T

H

H

A

A

S

S

M

I

A

P

P

A

E

E

A

T

R

R

R

A

T

D

A

L

G

G

I

I

A

G

D

D

S

S

L

L

L

I

R

R

I

L

S

S

V

V

G

G

I

V

E

E

D

A

G

L

D

E

D

D

L

L

V

Q

R

A

D

D

V

L

D

A

A

Q

G

A

L

L

A

A

R

K

V

I

binding pyridoxal-5'-phosphate: G66 (= G81), L67 (≠ M82), Y90 (= Y105), D163 (= D177), F166 (= F180), S185 (= S199), T187 (= T201), K188 (= K202)

4ixsB Native structure of xometc at ph 5.2 (see paper)
48% identity, 94% coverage: 9:384/399 of query aligns to 2:371/372 of 4ixsB

query
sites
4ixsB

S

S

T

L

S

A

T

T

C

L

A

A

V

I

R

H

A

G

G

G

I

Q

E

S

S

P

D

D

T

P

Q

S

H

T

G

G

A

A

V

V

V

M

P

P

P

P

L

I

H

Y

L

A

S

T

T

S

N

T

Y

Y

A

A

F

-

E

-

G

-

L

-

G

-

G

-

K

-

R

-

A

Q

Y

S

D

S

Y

P

S

S

R
|
R

S

T

G

H

N

N

P

P

T

T

R

R

D

F

L

A

L

Y

G

E

N

R

A

C

L

V

A

A

E

A

L

L

E

E

Q

G

G

G

A

T

G

R

A

A

V

F

V

A

T

F

A

A

S

S

G

G

M

M

A

A

A

A

V

T

A

S

L

T

A

V

L

M

E

E

L

L

V

L

P

D

A

A

G

G

G

S

R

H

V

V

L

V

A

A

A

M

H

D

D

D

C

L

Y
|
Y

G

G

G

G

T

T

W

F

R

R

L

L

L

F

D

E

A

R

W

V

A

R

K

R

K

R

-

T

G

A

R

G

F

L

T

D

L

F

E

S

F

F

A

V

D

D

L

L

T

T

D

D

R

P

V

A

A

A

L

F

A

K

A

A

G

A

L

I

A

R

S

A

K

D

P

T

S

K

L

M

V

V

W

W

V

I

E

E

T

T

P

P

S

T

N

N

P

P

L

M

L

L

R

K

I

L

T

V

D

D

V

I

R

A

H

A

V

I

A

A

Q

V

A

I

A

A

H

R

A

K

A

H

G

G

A

L

L

L

V

T

V

V

V

V

D
|
D

N

N

T

T

F

F

L

A

S

S

P

P

A

M

L

L

Q

Q

Q

R

P

P

L

L

V

S

L

L

G

G

A

A

D

D

V

L

V

V

V

V

H

H

S

S

T

A

T

T

K
|
K

Y

Y

I

L

N

N

G

G

H

H

S

S

D

D

V

M

V

V

G

G

G

G

A

I

V

A

V

V

A

V

R

G

D

D

Q

N

A

A

-

E

V

L

A

A

D

E

Q

Q

L

M

K

A

W

F

W

L

G

Q

N

N

C

S

N

I

G

G

L

G

T

V

G

Q

A

G

P

P

F

F

D

D

S

S

F

F

L

L

T

A

L

L

R

R

G

G

L

L

R

K

T

T

L