SitesBLAST
Comparing N515DRAFT_4421 FitnessBrowser__Dyella79:N515DRAFT_4421 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P00962 Glutamine--tRNA ligase; Glutaminyl-tRNA synthetase; GlnRS; EC 6.1.1.18 from Escherichia coli (strain K12) (see 7 papers)
54% identity, 96% coverage: 23:585/587 of query aligns to 10:551/554 of P00962
- R31 (= R44) mutation R->A,K: Decreased affinity for glutamine and catalytic activity.
- EPN 35:37 (= EPN 48:50) binding
- 41:47 (vs. 54:60, 86% identical) binding
- D67 (= D80) binding
- Y212 (= Y224) binding
- C230 (= C242) mutation to R: Decreases catalytic activity 1000-fold, but has no effect on affinity for glutamine. Loss of catalytic activity; when associated with I-256.
- T231 (= T243) binding
- Q256 (= Q286) mutation to I: Loss of catalytic activity; when associated with R-230.
- RL 261:262 (= RL 291:292) binding
- MSK 269:271 (≠ TSK 299:301) binding
- 317:324 (vs. 347:354, 38% identical) Interaction with tRNA
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
4jyzA Crystal structure of e. Coli glutaminyl-tRNA synthetase bound to atp and native tRNA(gln) containing the cmnm5s2u34 anticodon wobble base (see paper)
54% identity, 96% coverage: 23:583/587 of query aligns to 3:542/542 of 4jyzA
- active site: E28 (= E48), R254 (= R291), K264 (= K301)
- binding adenosine-5'-triphosphate: H34 (= H54), G36 (= G56), H37 (= H57), S40 (≠ A60), T224 (= T243), R254 (= R291), L255 (= L292), M262 (≠ T299), K264 (= K301)
- binding : Q7 (= Q27), E28 (= E48), T62 (= T82), N63 (= N83), P120 (≠ A139), R124 (= R143), R127 (= R146), T129 (= T148), L130 (= L149), G162 (= G181), C165 (≠ T184), P175 (≠ G194), F176 (≠ N195), I177 (= I196), V183 (≠ A202), R186 (= R205), K188 (≠ R207), M204 (= M223), Y205 (= Y224), F227 (= F246), Q228 (≠ E247), D229 (= D248), R232 (≠ P251), I307 (≠ L344), T310 (≠ S347), K311 (= K348), Q312 (= Q349), D313 (≠ N350), N314 (≠ S351), T315 (≠ V352), E317 (≠ D354), A319 (= A356), S320 (≠ I357), S323 (≠ N360), N330 (≠ D367), R335 (= R372), N364 (≠ K402), Q393 (≠ G431), K395 (≠ H433), R396 (= R434), R404 (= R442), R406 (= R444), S437 (≠ Q475), K438 (≠ G476), V449 (≠ T487), Q511 (= Q552), E513 (= E554), R514 (= R555), L538 (= L579), R539 (= R580), T541 (= T582), W542 (= W583)
1o0cA Crystal structure of l-glutamate and ampcpp bound to glutamine aminoacyl tRNA synthetase (see paper)
53% identity, 95% coverage: 23:582/587 of query aligns to 2:529/529 of 1o0cA
- binding adenosine monophosphate: F24 (= F45), P26 (= P47), H33 (= H54), G35 (= G56), H36 (= H57), S39 (≠ A60), T223 (= T243), R253 (= R291), L254 (= L292), K263 (= K301)
- binding glutamic acid: R23 (= R44), Y204 (= Y224), H208 (= H228), C222 (= C242)
- binding : Q6 (= Q27), E27 (= E48), T61 (= T82), N62 (= N83), P119 (≠ A139), R123 (= R143), R126 (= R146), T128 (= T148), L129 (= L149), T130 (= T150), G161 (= G181), P174 (≠ G194), F175 (≠ N195), I176 (= I196), R185 (= R205), K187 (≠ R207), M203 (= M223), Y204 (= Y224), F226 (= F246), Q227 (≠ E247), D228 (= D248), R231 (≠ P251), I306 (≠ L344), T309 (≠ S347), K310 (= K348), Q311 (= Q349), N313 (≠ S351), T314 (≠ V352), E316 (≠ D354), A318 (= A356), S319 (≠ I357), S322 (≠ N360), N329 (≠ D367), R334 (= R372), P362 (= P401), Q392 (≠ G431), K394 (≠ H433), R395 (= R434), R403 (= R442), R405 (= R444), N406 (≠ G445), L435 (≠ R474), Q499 (= Q552), E501 (= E554), R502 (= R555), R527 (= R580)
1o0bA Crystal structure of l-glutamine and ampcpp bound to glutamine aminoacyl tRNA synthetase (see paper)
53% identity, 95% coverage: 23:582/587 of query aligns to 2:529/529 of 1o0bA
- binding adenosine monophosphate: F24 (= F45), P26 (= P47), H33 (= H54), G35 (= G56), H36 (= H57), S39 (≠ A60), R253 (= R291), L254 (= L292)
- binding glutamine: P25 (= P46), D59 (= D80), Y204 (= Y224), H208 (= H228), C222 (= C242), F226 (= F246)
- binding : Q6 (= Q27), E27 (= E48), D59 (= D80), T61 (= T82), N62 (= N83), P119 (≠ A139), R123 (= R143), R126 (= R146), T128 (= T148), L129 (= L149), T130 (= T150), G161 (= G181), P174 (≠ G194), F175 (≠ N195), I176 (= I196), V182 (≠ A202), R185 (= R205), K187 (≠ R207), M203 (= M223), Y204 (= Y224), F226 (= F246), Q227 (≠ E247), D228 (= D248), R231 (≠ P251), I306 (≠ L344), T309 (≠ S347), K310 (= K348), Q311 (= Q349), D312 (≠ N350), N313 (≠ S351), T314 (≠ V352), E316 (≠ D354), A318 (= A356), S322 (≠ N360), N329 (≠ D367), R334 (= R372), P362 (= P401), N363 (≠ K402), Q392 (≠ G431), K394 (≠ H433), R395 (= R434), R403 (= R442), R405 (= R444), N406 (≠ G445), L435 (≠ R474), V437 (≠ T487), Q499 (= Q552), E501 (= E554), R502 (= R555), R527 (= R580)
1gtsA Structural basis for transfer RNA aminoaceylation by escherichia coli glutaminyl-tRNA synthetase (see paper)
53% identity, 95% coverage: 23:582/587 of query aligns to 2:529/529 of 1gtsA
- binding adenosine monophosphate: E27 (= E48), G35 (= G56), H36 (= H57), S39 (≠ A60), T223 (= T243), R253 (= R291), L254 (= L292), K263 (= K301)
- binding : Q6 (= Q27), E27 (= E48), D59 (= D80), T61 (= T82), N62 (= N83), P119 (≠ A139), R126 (= R146), L129 (= L149), G161 (= G181), P174 (≠ G194), F175 (≠ N195), I176 (= I196), V182 (≠ A202), R185 (= R205), K187 (≠ R207), M203 (= M223), Y204 (= Y224), F226 (= F246), Q227 (≠ E247), D228 (= D248), I306 (≠ L344), T309 (≠ S347), K310 (= K348), Q311 (= Q349), N313 (≠ S351), T314 (≠ V352), E316 (≠ D354), A318 (= A356), S322 (≠ N360), N329 (≠ D367), R334 (= R372), Q392 (≠ G431), Y393 (≠ F432), K394 (≠ H433), R395 (= R434), R403 (= R442), R405 (= R444), N406 (≠ G445), V437 (≠ T487), E501 (= E554), R502 (= R555), R527 (= R580), T529 (= T582)
1exdA Crystal structure of a tight-binding glutamine tRNA bound to glutamine aminoacyl tRNA synthetase (see paper)
53% identity, 95% coverage: 23:582/587 of query aligns to 2:529/529 of 1exdA
- active site: E27 (= E48), R253 (= R291), K263 (= K301)
- binding adenosine-5'-monophosphate: F24 (= F45), P26 (= P47), E27 (= E48), G35 (= G56), H36 (= H57), S39 (≠ A60), R253 (= R291), L254 (= L292), K263 (= K301)
- binding sulfate ion: V260 (≠ I298), M261 (≠ T299), S262 (= S300), K265 (= K303)
- binding : E27 (= E48), D59 (= D80), T61 (= T82), N62 (= N83), P119 (≠ A139), R123 (= R143), R126 (= R146), L129 (= L149), T130 (= T150), G161 (= G181), P174 (≠ G194), F175 (≠ N195), I176 (= I196), V182 (≠ A202), R185 (= R205), K187 (≠ R207), M203 (= M223), Y204 (= Y224), F226 (= F246), Q227 (≠ E247), D228 (= D248), I306 (≠ L344), T309 (≠ S347), K310 (= K348), Q311 (= Q349), D312 (≠ N350), N313 (≠ S351), T314 (≠ V352), E316 (≠ D354), A318 (= A356), S319 (≠ I357), S322 (≠ N360), N329 (≠ D367), R334 (= R372), P362 (= P401), Q392 (≠ G431), K394 (≠ H433), R395 (= R434), R403 (= R442), R405 (= R444), N406 (≠ G445), V437 (≠ T487), Q499 (= Q552), E501 (= E554), R502 (= R555), L526 (= L579), R527 (= R580)
1euqA Crystal structure of glutaminyl-tRNA synthetase complexed with a tRNA- gln mutant and an active-site inhibitor (see paper)
53% identity, 95% coverage: 23:582/587 of query aligns to 2:529/529 of 1euqA
- active site: E27 (= E48), R253 (= R291), K263 (= K301)
- binding 5'-o-[n-(l-glutaminyl)-sulfamoyl]adenosine: P26 (= P47), H33 (= H54), G35 (= G56), H36 (= H57), S39 (≠ A60), Y204 (= Y224), H208 (= H228), C222 (= C242), F226 (= F246), R253 (= R291), K263 (= K301)
- binding : Q6 (= Q27), E27 (= E48), D59 (= D80), T61 (= T82), N62 (= N83), P119 (≠ A139), R123 (= R143), R126 (= R146), L129 (= L149), G161 (= G181), C164 (≠ T184), P174 (≠ G194), F175 (≠ N195), I176 (= I196), V182 (≠ A202), R185 (= R205), K187 (≠ R207), M203 (= M223), Y204 (= Y224), F226 (= F246), I306 (≠ L344), T309 (≠ S347), K310 (= K348), Q311 (= Q349), N313 (≠ S351), T314 (≠ V352), E316 (≠ D354), A318 (= A356), S322 (≠ N360), N329 (≠ D367), R334 (= R372), N363 (≠ K402), Q392 (≠ G431), K394 (≠ H433), R403 (= R442), R405 (= R444), N406 (≠ G445), V437 (≠ T487), Q499 (= Q552), E501 (= E554), R502 (= R555), L526 (= L579), R527 (= R580)
2re8A Glutaminyl-tRNA synthetase mutant c229r with bound analog 5'-o-[n-(l- glutamyl)-sulfamoyl]adenosine (see paper)
53% identity, 95% coverage: 23:582/587 of query aligns to 2:529/529 of 2re8A
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: P25 (= P46), P26 (= P47), E27 (= E48), G35 (= G56), H36 (= H57), D59 (= D80), Y204 (= Y224), H208 (= H228), R222 (≠ C242), T223 (= T243), R253 (= R291), L254 (= L292), K263 (= K301)
- binding : Q6 (= Q27), E27 (= E48), T61 (= T82), N62 (= N83), L117 (= L137), P119 (≠ A139), R123 (= R143), R126 (= R146), T128 (= T148), L129 (= L149), G161 (= G181), P174 (≠ G194), F175 (≠ N195), I176 (= I196), V182 (≠ A202), R185 (= R205), M203 (= M223), Y204 (= Y224), F226 (= F246), D228 (= D248), I306 (≠ L344), T309 (≠ S347), K310 (= K348), Q311 (= Q349), N313 (≠ S351), T314 (≠ V352), E316 (≠ D354), S319 (≠ I357), S322 (≠ N360), N329 (≠ D367), R334 (= R372), P362 (= P401), N363 (≠ K402), Q392 (≠ G431), K394 (≠ H433), R395 (= R434), R403 (= R442), R405 (= R444), N406 (≠ G445), L435 (≠ R474), Q499 (= Q552), E501 (= E554), R502 (= R555), R527 (= R580)
5zdkA Crystal structure analysis of ttqrs in complex with atp (see paper)
47% identity, 95% coverage: 24:580/587 of query aligns to 8:543/543 of 5zdkA
- binding adenosine-5'-triphosphate: F29 (= F45), P31 (= P47), E32 (= E48), G40 (= G56), H41 (= H57), T223 (= T243), R259 (= R291), L260 (= L292)
- binding cesium ion: W366 (≠ H400), P367 (= P401), I370 (vs. gap), E373 (≠ F406)
P47897 Glutamine--tRNA ligase; Glutaminyl-tRNA synthetase; GlnRS; EC 6.1.1.18 from Homo sapiens (Human) (see 2 papers)
43% identity, 92% coverage: 41:581/587 of query aligns to 264:770/775 of P47897
- R403 (= R186) to W: in MSCCA; results in loss of glutaminyl-tRNA aminoacylation activity; impairs protein folding; does not interact with RARS1; results in reduced protein solubility; dbSNP:rs587777332
- R515 (= R320) to W: in MSCCA; results in loss of glutaminyl-tRNA aminoacylation activity; impairs protein folding; results in reduced protein solubility; dbSNP:rs587777334
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 45 G → V: in MSCCA; results in reduced glutaminyl-tRNA aminoacylation activity; does not affect interaction with RARS1; dbSNP:rs587777331
- 57 Y → H: in MSCCA; results in reduced glutaminyl-tRNA aminoacylation activity; does not affect interaction with RARS1; dbSNP:rs587777333
- 175 H→A: Decreases catalytic efficiency about 60-fold.
4h3sA The structure of glutaminyl-tRNA synthetase from saccharomyces cerevisiae (see paper)
40% identity, 93% coverage: 41:585/587 of query aligns to 38:585/585 of 4h3sA
O13775 Probable glutamate--tRNA ligase, cytoplasmic; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
34% identity, 93% coverage: 32:574/587 of query aligns to 201:702/716 of O13775
Sites not aligning to the query:
- 190 modified: Phosphoserine
Q8CGC7 Bifunctional glutamate/proline--tRNA ligase; Bifunctional aminoacyl-tRNA synthetase; EC 6.1.1.17; EC 6.1.1.15 from Mus musculus (Mouse) (see 2 papers)
34% identity, 91% coverage: 32:563/587 of query aligns to 189:682/1512 of Q8CGC7
Sites not aligning to the query:
- 999 modified: Phosphoserine; by RPS6KB1; S→A: Loss of function in translation inhibition. Loss of interaction with SLC27A1. Mutant mice have no apparent developmental defect but display reduced adiposity associated with decreased insulin levels and adipocytes size. They also display increased lipolysis and fatty acid beta-oxidation and an extended lifespan. Adipocytes display decreased insulin-stimulated long-chain fatty acid uptake.; S→D: Constitutively active in translation inhibition (phosphomimetic). Mutant mice have no apparent developmental defect and do not display overt phenotype related to adiposity or lifespan.
P07814 Bifunctional glutamate/proline--tRNA ligase; Bifunctional aminoacyl-tRNA synthetase; Cell proliferation-inducing gene 32 protein; Glutamatyl-prolyl-tRNA synthetase; EC 6.1.1.17; EC 6.1.1.15 from Homo sapiens (Human) (see 13 papers)
33% identity, 94% coverage: 10:563/587 of query aligns to 171:682/1512 of P07814
- K300 (≠ R143) modified: N6-malonyllysine; alternate
- D308 (≠ N155) to E: in dbSNP:rs2230301
- Q334 (≠ G181) to H: in dbSNP:rs1063236
Sites not aligning to the query:
- 886 modified: Phosphoserine; by CDK5; S→A: Abolishes release from the aminoacyl-tRNA synthetase multienzyme complex and association with the GAIT complex upon interferon-gamma treatment. Abolishes interaction with SYNCRIP.; S→D: Not active in translation inhibition (phosphomimetic) and abolishes GAIT complex association with eiF4G. No effect on interaction with SYNCRIP.
- 999 modified: Phosphoserine; by RPS6KB1; S→A: Not active in translation inhibition, abolishes release from the aminoacyl-tRNA synthetase multienzyme complex and association with the GAIT complex upon interferon-gamma treatment.; S→D: Active in translation inhibition (phosphomimetic). No effect on GAIT complex association with eiF4G.
- 1043 I → V: in dbSNP:rs5030752
- 1097 F→A: Almost complete loss of prolyl-tRNA ligase activity.; F→W: No effect on prolyl-tRNA ligase activity. Decreases inhibition by halofuginone.
- 1115 P → R: in HLD15; slightly decreased protein level; does not affect multisynthetase complex assembly; dbSNP:rs1288116010
- 1121:1123 binding
- 1126 M → T: in HLD15; uncertain significance; small decrease in aminoacylation activity; dbSNP:rs1474000585
- 1152 binding ; binding ; R→K: No effect on prolyl-tRNA ligase activity. Decreases inhibition by halofuginone.; R→L: Almost complete loss of prolyl-tRNA ligase activity.
- 1154 binding
- 1160 P → S: in HLD15; uncertain significance; dbSNP:rs898824971
- 1163 binding
- 1164 binding
- 1237 binding ; binding
- 1240 binding
- 1242 binding
- 1276 binding
- 1278 binding
- 1448 binding
- 1453 binding
- 1495 binding
- 1497 binding
P46655 Glutamate--tRNA ligase, cytoplasmic; Glutamyl-tRNA synthetase; (c)ERS; GluRS; P85; EC 6.1.1.17 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
32% identity, 92% coverage: 32:573/587 of query aligns to 193:692/708 of P46655
Sites not aligning to the query:
- 148 R→A: Abolishes interaction with ARC1.
P28668 Bifunctional glutamate/proline--tRNA ligase; Bifunctional aminoacyl-tRNA synthetase; EC 6.1.1.17; EC 6.1.1.15 from Drosophila melanogaster (Fruit fly) (see 2 papers)
33% identity, 90% coverage: 32:562/587 of query aligns to 195:688/1714 of P28668
Sites not aligning to the query:
- 170:754 Glutamate--tRNA ligase
- 1110 modified: Phosphoserine
- 1207:1714 Proline--tRNA ligase
7waoA Glutamyl-tRNA synthetase from plasmodium falciparum (pfers) in complex with mn (see paper)
31% identity, 91% coverage: 33:565/587 of query aligns to 2:488/502 of 7waoA
7wajA Glutamyl-tRNA synthetase from plasmodium falciparum (pfers) complexed with atp and co (see paper)
31% identity, 91% coverage: 33:565/587 of query aligns to 2:479/492 of 7wajA
Sites not aligning to the query:
7wakA Glutamyl-tRNA synthetase from plasmodium falciparum (pfers) in complex with adp (see paper)
30% identity, 91% coverage: 33:565/587 of query aligns to 1:468/481 of 7wakA
3aiiA Archaeal non-discriminating glutamyl-tRNA synthetase from methanothermobacter thermautotrophicus (see paper)
30% identity, 90% coverage: 32:558/587 of query aligns to 3:438/455 of 3aiiA
Query Sequence
>N515DRAFT_4421 FitnessBrowser__Dyella79:N515DRAFT_4421
MSTEPAAAETTSASAAPAEGQRDFVRQIIRDDLANGKHHAIRTRFPPEPNGYLHIGHAKA
ICLNFGIAREFAGWCNLRLDDTNPGKEDPEFVEGIKDDVRWLGYDWHDLRHASDYFEVFY
RAAVKLIEDGHAYVDDLNADEMREYRGTLTAPGRNSPHRERSVAENLDLFRRMREGEFAD
GAKTLRAKIDMTSGNINMRDPAIYRIRRIPHQNTGDAWPIYPMYDYAHCLSDALEGITHS
LCTLEFEDHRPLYDWFVDKVDLANHPELWTHLREGGFKTEPAKPRQIEFSRLNLSYCITS
KRKLAQLVNERLVDGWDDPRMNTLRGLRRRGFTPGGIRLLIERLGVSKQNSVIDYAIFEN
CVREDLDASAPRRLAVLDPLKLVITNLAEDHEETLSFANHPKDESFGTREVPFARELWIE
REDFAEVPPKGFHRLKPEGEVRLRGVGIVKCEELVKDAAGNVIELRCTLDLDSRQGMPGA
DRKVKGTIHWVSARHAVAAEVRVYDRLFNVAAPDDESDGKTWIEHINPEAKRIVQAWVEP
SAAATAPEQRFQFERLGYFVADRVEHGAQKPIFNRTVTLRDTWAKQA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory