SitesBLAST

P10880 Shikimate kinase 2; SK 2; Shikimate kinase II; SKII; EC 2.7.1.71 from Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi) (see paper)
30% identity, 85% coverage: 3:143/165 of query aligns to 5:151/173 of P10880

query
sites
P10880

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C13 (= C11) mutation to S: 66% of wild-type activity. Increase in substrates affinity.
K15 (= K13) mutation to M: Loss of activity. Increased thermostability.
D34 (= D32) mutation to N: Loss of activity.

Sites not aligning to the query:

162 C→S: No effect on activity and substrates affinity.

1e6cA K15m mutant of shikimate kinase from erwinia chrysanthemi (see paper)
30% identity, 85% coverage: 3:143/165 of query aligns to 5:151/170 of 1e6cA

query
sites
1e6cA

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binding phosphate ion: R11 (≠ P9), G12 (= G10), M15 (≠ K13), T16 (≠ S14)

1shkA The three-dimensional structure of shikimate kinase from erwinia chrysanthemi (see paper)
30% identity, 85% coverage: 3:143/165 of query aligns to 5:136/158 of 1shkA

query
sites
1shkA

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binding magnesium ion: R92 (≠ K90), A93 (≠ K91), G95 (= G93), Q129 (≠ I136), A132 (≠ C139)

2iyvA Shikimate kinase from mycobacterium tuberculosis in complex with adp, open lid (conf. B) (see paper)
33% identity, 92% coverage: 4:155/165 of query aligns to 5:163/179 of 2iyvA

query
sites
2iyvA

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binding adenosine-5'-diphosphate: G11 (= G10), G13 (= G12), K14 (= K13), S15 (= S14), T16 (= T15), R109 (≠ E107), R152 (≠ L144), P154 (≠ D146)

4bqsA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with adp and a shikimic acid derivative. (see paper)
33% identity, 92% coverage: 4:155/165 of query aligns to 5:163/169 of 4bqsA

query
sites
4bqsA

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binding adenosine-5'-diphosphate: P10 (= P9), G11 (= G10), G13 (= G12), K14 (= K13), S15 (= S14), T16 (= T15), R109 (≠ E107), R152 (≠ L144), P154 (≠ D146)
binding (1R,6R,10S)-6,10-dihydroxy-2-oxabicyclo[4.3.1]deca-4(Z),7-diene-8-carboxylic acid: D33 (= D32), R135 (= R131)

2iyyA Shikimate kinase from mycobacterium tuberculosis in complex with shikimate-3-phosphate and so4 (see paper)
33% identity, 92% coverage: 4:155/165 of query aligns to 5:163/169 of 2iyyA

query
sites
2iyyA

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binding magnesium ion: E37 (= E36), S43 (≠ K42), I44 (= I43)
binding phosphate ion: K14 (= K13), G79 (= G77)
binding shikimate-3-phosphate: K14 (= K13), D33 (= D32), R57 (= R55), G79 (= G77), G80 (= G78), L118 (= L116), R135 (= R131)

3bafA Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with amp-pnp
33% identity, 92% coverage: 4:155/165 of query aligns to 5:163/165 of 3bafA

query
sites
3bafA

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binding phosphoaminophosphonic acid-adenylate ester: P10 (= P9), G13 (= G12), K14 (= K13), S15 (= S14), T16 (= T15), R109 (≠ E107), R116 (= R114)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D32), R135 (= R131)

2dfnA Structure of shikimate kinase from mycobacterium tuberculosis complexed with adp and shikimate at 1.9 angstrons of resolution (see paper)
33% identity, 92% coverage: 4:155/165 of query aligns to 5:163/165 of 2dfnA

query
sites
2dfnA

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T

T

R

S

E

P

K

G

N

V

I

R

L

A

C

A

L

L

K

A

K

G

K

H

G

T

K

V

I

V

V

Y

F

L

I

E

N

I

R

S

P

A

E

E

K

G

I

V

M

R

E
x
R

D

T

V

G

D

G

I

N

E

T

N

V

R
|
R

P

P

L

L

E

A

H

-

G

G

K

P

S

D

R

R

I

A

L

E

K

K

-

Y

-

R

-

A

L

L

Y

M

E

A

E

K

R
|
R

Y

A

K

P

L

L

Y

Y

I

R

N

R

Y

V

C

A

D

T

Y

M

Q

R

V

V

-

D

-

T

-

N

-

R

L
x
R

N

N

D
x
P

G

G

S

A

I

V

D

V

R

R

V

H

V

I

L

L

S

S

binding adenosine-5'-diphosphate: G11 (= G10), S12 (≠ C11), G13 (= G12), K14 (= K13), S15 (= S14), T16 (= T15), R109 (≠ E107), R116 (= R114), R152 (≠ L144), P154 (≠ D146)
binding chloride ion: P10 (= P9), K14 (= K13), G79 (= G77)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D32), I44 (= I43), F56 (= F54), R57 (= R55), G78 (= G76), G79 (= G77), G80 (= G78), R135 (= R131)

1we2A Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with mgadp and shikimic acid (see paper)
33% identity, 92% coverage: 4:155/165 of query aligns to 5:163/165 of 1we2A

query
sites
1we2A

V

V

L

L

I

V

G

G

M

L

P
|
P

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

I

I

G

G

K

R

I

R

L

L

S

A

K

K

E

A

L

L

N

G

M

V

K

G

F

L

Y

L

D

D

A

T

D
|
D

K

V

Y

A

L

I

E

E

D

Q

R

R

N

T

N

G

M

R

K

S

I
|
I

S

A

D

D

M

I

F
|
F

K

A

R

T

-

D

G

G

E

E

E

Q

F

E

F

F

R

R

N

R

L

I

E

E

S

E

A

D

A

V

L

V

K

R

E

A

L

A

S

L

F

A

E

D

E

H

N

D

S

G

V

V

I

L

A

S

T

L

G
|
G

A

A

V

V

T

T

R

S

E

P

K

G

N

V

I

R

L

A

C

A

L

L

K

A

K

G

K

H

G

T

K

V

I

V

V

Y

F

L

I

E

N

I

R

S

P

A

E

E

K

G

I

V

M

R

E
x
R

D

T

V

G

D

G

I

N

E

T

N

V

R
|
R

P

P

L
|
L

L

L

E

A

H

-

G

G

K

P

S

D

R

R

I

A

L

E

K

K

-

Y

-

R

-

A

L

L

Y

M

E

A

E

K

R
|
R

Y

A

K

P

L

L

Y

Y

I

R

N

R

Y

V

C

A

D

T

Y

M

Q

R

V

V

-

D

-

T

-

N

-

R

L

R

N

N

D

P

G

G

S

A

I

V

D

V

R

R

V

H

V

I

L

L

S

S

binding adenosine-5'-diphosphate: P10 (= P9), G11 (= G10), G13 (= G12), K14 (= K13), S15 (= S14), T16 (= T15), R109 (≠ E107), R116 (= R114)
binding 3-dehydroshikimate: D33 (= D32), I44 (= I43), F48 (= F47), G78 (= G76), G79 (= G77), G80 (= G78), L118 (= L116), R135 (= R131)

1zyuA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and amppcp at 2.85 angstrom resolution (see paper)
33% identity, 92% coverage: 4:155/165 of query aligns to 5:163/168 of 1zyuA

query
sites
1zyuA

V

V

L

L

I

V

G

G

M

L

P
|
P

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

I

I

G

G

K

R

I

R

L

L

S

A

K

K

E

A

L

L

N

G

M

V

K

G

F

L

Y

L

D

D

A

T

D
|
D

K

V

Y

A

L

I

E

E

D

Q

R

R

N

T

N

G

M

R

K

S

I

I

S

A

D

D

M

I

F

F

K

A

R

T

-

D

G

G

E

E

E

Q

F

E

F

F

R
|
R

N

R

L

I

E

E

S

E

A

D

A

V

L

V

K

R

E

A

L

A

S

L

F

A

E

D

E

H

N

D

S

G

V

V

I

L

A

S

T

L

G

G

G
|
G

A

A

V

V

T

T

R

S

E

P

K

G

N

V

I

R

L

A

C

A

L

L

K

A

K

G

K

H

G

T

K

V

I

V

V

Y

F

L

I

E

N

I

R

S

P

A

E

E

K

G

I

V

M

R

E
x
R

D

T

V

G

D

G

I

N

E

T

N

V

R

R

P
|
P

L
|
L

L

L

E

A

H

-

G

G

K

P

S

D

R

R

I

A

L

E

K

K

-

Y

-

R

-

A

L

L

Y

M

E

A

E

K

R
|
R

Y

A

K

P

L

L

Y

Y

I

R

N

R

Y

V

C

A

D

T

Y

M

Q

R

V

V

-

D

-
x
T

-

N

-

R

L
x
R

N

N

D
x
P

G

G

S

A

I

V

D

V

R

R

V

H

V

I

L

L

S

S

binding phosphomethylphosphonic acid adenylate ester: P10 (= P9), G11 (= G10), G13 (= G12), K14 (= K13), S15 (= S14), T16 (= T15), R109 (≠ E107), T149 (vs. gap), R152 (≠ L144), P154 (≠ D146)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D32), R57 (= R55), G80 (= G78), P117 (= P115), L118 (= L116), R135 (= R131)

2iywA Shikimate kinase from mycobacterium tuberculosis in complex with mgatp, open lid (conf. B) (see paper)
33% identity, 92% coverage: 4:155/165 of query aligns to 5:163/178 of 2iywA

query
sites
2iywA

V

V

L

L

I

V

G

G

M

L

P

P

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

I

I

G

G

K

R

I

R

L

L

S

A

K

K

E

A

L

L

N

G

M

V

K

G

F

L

Y

L

D

D

A

T

D

D

K

V

Y

A

L

I

E

E

D

Q

R

R

N

T

N

G

M

R

K

S

I

I

S

A

D

D

M

I

F

F

K

A

R

T

-

D

G

G

E

E

E

Q

F

E

F

F

R

R

N

R

L

I

E

E

S

E

A

D

A

V

L

V

K

R

E

A

L

A

S

L

F

A

E

D

E

H

N

D

S

G

V

V

I

L

A

S

T

L

G

G

A

A

V

V

T

T

R

S

E

P

K

G

N

V

I

R

L

A

C

A

L

L

K

A

K

G

K

H

G

T

K

V

I

V

V

Y

F

L

I

E

N

I

R

S

P

A

E

E

K

G

I

V

M

R

E
x
R

D

T

V

G

D

G

I

N

E

T

N

V

R

R

P

P

L

L

E

A

H

-

G

G

K

P

S

D

R

R

I

A

L

E

K

K

-

Y

-

R

-

A

L

L

Y

M

E

A

E

K

R

R

Y

A

K

P

L

L

Y

Y

I

R

N

R

Y

V

C

A

D

T

Y

M

Q

R

V

V

-

D

-

T

-

N

-

R

L
x
R

N

N

D
x
P

G

G

S

A

I

V

D

V

R

R

V

H

V

I

L

L

S

S

binding adenosine-5'-triphosphate: G11 (= G10), G13 (= G12), K14 (= K13), S15 (= S14), T16 (= T15), R109 (≠ E107), R152 (≠ L144), P154 (≠ D146)

1u8aA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and adp at 2.15 angstrom resolution (see paper)
33% identity, 92% coverage: 4:155/165 of query aligns to 5:161/163 of 1u8aA

query
sites
1u8aA

V

V

L

L

I

V

G

G

M

L

P

P

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

I

I

G

G

K

R

I

R

L

L

S

A

K

K

E

A

L

L

N

G

M

V

K

G

F

L

Y

L

D

D

A

T

D
|
D

K

V

Y

A

L

I

E

E

D

Q

R

R

N

T

N

G

M

R

K

S

I

I

S

A

D

D

M

I

F
|
F

K

A

R

T

-

D

G

G

E

E

E

Q

F

E

F

F

R
|
R

N

R

L

I

E

E

S

E

A

D

A

V

L

V

K

R

E

A

L

A

S

L

F

A

E

D

E

H

N

D

S

G

V

V

I

L

A

S

T

L

G

G

G
|
G

A

A

V

V

T

T

R

S

E

P

K

G

N

V

I

R

L

A

C

A

L

L

K

A

K

G

K

H

G

T

K

V

I

V

V

Y

F

L

I

E

N

I

R

S

P

A

E

E

K

G

I

V

M

R

E
x
R

D

T

V

G

D

G

I

V

E

-

N

-

R

R

P

P

L
|
L

L

L

E

A

H

-

G

G

K

P

S

D

R

R

I

A

L

E

K

K

-

Y

-

R

-

A

L

L

Y

M

E

A

E

K

R
|
R

Y

A

K

P

L

L

Y

Y

I

R

N

R

Y

V

C

A

D

T

Y

M

Q

R

V

V

-

D

-

T

-

N

-

R

L
x
R

N

N

D
x
P

G

G

S

A

I

V

D

V

R

R

V

H

V

I

L

L

S

S

binding adenosine-5'-diphosphate: G11 (= G10), G13 (= G12), K14 (= K13), S15 (= S14), T16 (= T15), R109 (≠ E107), R150 (≠ L144), P152 (≠ D146)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D32), F48 (= F47), R57 (= R55), G79 (= G77), G80 (= G78), L116 (= L116), R133 (= R131)

P07547 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
40% identity, 69% coverage: 2:115/165 of query aligns to 865:986/1583 of P07547

query
sites
P07547

N

S

L

I

V

Y

L

I

I

I

G

G

M

M

P

R

G

G

C

A

G

G

K

K

S

S

T

T

I

A

G

G

K

N

I

W

L

V

S

S

K

K

E

A

L

L

N

N

M

R

K

P

F

F

Y

V

D

D

A

L

D

D

K

T

Y

E

L

L

E

E

D

T

R

V

N

E

N

G

M

M

K

T

I

I

S

P

D

D

M

I

F

I

K

K

-

T

R

R

G

G

E

W

E

Q

F

G

F

F

R

R

N

N

L

A

E

E

S

L

A

E

A

I

L

L

K

K

E

R

L

-

S

T

F

L

E

K

E

E

N

R

S

S

-

R

-

G

-

Y

V

V

I

F

A

A

T

C

G

G

A

V

V

V

T

E

R

M

E

P

K

E

N

A

I

R

L

K

C

L

L

L

-

T

-

D

-

Y

-

H

K

K

K

T

K

K

G

G

K

N

I

V

V

L

F

L

I

L

N

M

R

R

P

D

P

I

E

K

K

K

I

I

M

M

E

D

D

F

V

L

D

S

I

I

E

D

-

K

N

T

R

R

P

P

Sites not aligning to the query:

44:46 binding
81:84 binding
114:116 binding
119 binding
139:140 binding
161 binding
179:182 binding
190 binding
194 binding
271 binding
287 binding

7tbvA Crystal structure of the shikimate kinase + 3-dehydroquinate dehydratase + 3-dehydroshikimate dehydrogenase domains of aro1 from candida albicans (see paper)
31% identity, 85% coverage: 2:142/165 of query aligns to 5:145/682 of 7tbvA

query
sites
7tbvA

N

S

L

I

V

I

L

V

I

I

G

G

M

M

P

R

G

G

C

T

G

G

K

K

S

S

T

T

I

L

G

S

K

E

I

W

L

L

S

A

K

S

E

F

L

L

N

G

M

F

K

K

F

M

Y

L

D

D

A

M

D

D

K

K

Y

Y

L

L

E

E

D

E

R

K

N

L

N

G

M

T

K

G

I

I

S

K

D

S

M

L

F

I

K

K

-

A

R

K

G

G

E

W

E

E

F

Y

F

F

R

R

N

Q

L

E

E

E

S

A

A

I

A

V

L

A

K

K

E

E

-

C

-

F

L

T

S

K

F

F

E

S

E

K

N

G

S

Y

V

V

I

L

A

S

T

T

G

G

A

I

V

V

T

E

R

G

E

E

K

D

N

A

I

R

L

Q

C

Q

L

L

K

K

K

S

-

Y

-

A

-

D

-

N

K

G

G

G

K

I

I

V

V

L

F

H

I

L

N

H

R

R

P

-

E

-

K

-

I

-

M

-

E

-

D

-

V

-

D

D

I

L

E

D

N

E

R

S

P

S

L

E

L

V

E

Q

H

E

G

V

K

W

S

L

R

R

I

R

L

E

K

K

L

W

Y

Y

E

H

E

E

-

C

-

S

R

N

Y

Y

K

H

L

F

Y

Y

I

S

N

S

Y

H

C

C

D

S

Y

T

Q

E

Sites not aligning to the query:

binding magnesium ion: 300, 302, 329

1zuiA Structural basis for shikimate-binding specificity of helicobacter pylori shikimate kinase (see paper)
29% identity, 97% coverage: 2:161/165 of query aligns to 2:156/158 of 1zuiA

query
sites
1zuiA

N

H

L

L

V

V

L

L

I

I

G

G

M

F

P
x
M

G

G

C

S

G

G

K

K

S

S

T

S

I

L

G

A

K

Q

I

E

L

L

S

G

K

L

E

A

L

L

N

K

M

L

K

E

F

V

Y

L

D

D

A

T

D
|
D

K

M

Y

I

L

I

E

S

D

E

R

R

N

V

N

G

M

L

K

S

I

V

S

R

D

E

M

I

F
|
F

K

E

R

E

-

L

G

G

E

E

E

D

F

N

F

F

R
|
R

N

M

L

F

E

E

S

K

A

N

A

L

L

I

K

D

E

E

L

L

-

K

S

T

F

L

E

K

E

T

N

P

S

H

V

V

I

I

A

S

T

T

G
|
G

G

G

A

I

V

V

T

M

R

H

E

E

K

N

N

-

I

-

L

-

C

-

L

L

K

K

K

G

K

L

G

G

K

T

I

T

V

F

F

Y

I

L

N

K

R

M

P

D

P

F

E

E

K

T

I

L

M

I

E

K

D

R

V

L

D

N

I

Q

-

R

E
|
E

N

K

R
|
R

P

P

L

L

E

-

H

N

G

N

K

L

S

T

R

Q

I

A

L

K

K

E

L

L

Y

F

E

E

E

K

R
|
R

Y

Q

K

A

L

L

Y

Y

I

E

N

K

Y

N

C

A

D

S

Y

F

Q

I

V

I

L

D

N

A

D

R

G

G

S

G

I

L

D

N

R

N

V

-

L

-

S

S

I

L

K

K

K

Q

Y

V

L

L

E

Q

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: M9 (≠ P9), D32 (= D32), F47 (= F47), R56 (= R55), G78 (= G76), G79 (= G77), E111 (= E112), R113 (= R114), R129 (= R131)

3mufA Shikimate kinase from helicobacter pylori in complex with shikimate-3- phosphate and adp (see paper)
29% identity, 97% coverage: 2:161/165 of query aligns to 2:158/160 of 3mufA

query
sites
3mufA

N

H

L

L

V

V

L

L

I

I

G

G

M

F

P
x
M

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
x
S

I

L

G

A

K

Q

I

E

L

L

S

G

K

L

E

A

L

L

N

K

M

L

K

E

F

V

Y

L

D

D

A

T

D
|
D

K

M

Y

I

L

I

E

S

D

E

R

R

N

V

N

G

M

L

K

S

I

V

S

R

D

E

M

I

F

F

K

E

R

E

-

L

G

G

E

E

E

D

F

N

F

F

R
|
R

N

M

L

F

E

E

S

K

A

N

A

L

L

I

K

D

E

E

L

L

-

K

S

T

F

L

E

K

E

T

N

P

S

H

V

V

I

I

A

S

T

T

G
|
G

A

I

V

V

T

M

R

H

E

E

K

N

N

-

I

-

L

-

C

-

L

L

K

K

K

G

K

L

G

G

K

T

I

T

V

F

F

Y

I

L

N

K

R
x
M

P

D

P

F

E

E

K

T

I

L

M

I

E

K

D
x
R

V

L

D

N

I

Q

-

K

-

E

-

R

E

E

N

K

R
|
R

P

P

L

L

E

-

H

N

G

N

K

L

S

T

R

Q

I

A

L

K

K

E

L

L

Y

F

E

E

E

K

R
|
R

Y

Q

K

A

L

L

Y

Y

I

E

N

K

Y

N

C

A

D

S

Y

F

Q

I

V

I

L

D

N

A

D

R

G
|
G

S
x
G

I

L

D

N

R

N

V

-

L

-

S

S

I

L

K

K

K

Q

Y

V

L

L

E

Q

binding adenosine-5'-diphosphate: G10 (= G10), S11 (≠ C11), G12 (= G12), K13 (= K13), S14 (= S14), S15 (≠ T15), M98 (≠ R100), R106 (≠ D108), G147 (= G147), G148 (≠ S148)
binding shikimate-3-phosphate: M9 (≠ P9), D32 (= D32), R56 (= R55), G78 (= G76), G79 (= G77), G80 (= G78), R115 (= R114), R131 (= R131)

3n2eA Crystal structure of helicobactor pylori shikimate kinase in complex with nsc162535 (see paper)
28% identity, 97% coverage: 2:161/165 of query aligns to 3:159/168 of 3n2eA

query
sites
3n2eA

N

H

L

L

V

V

L

L

I

I

G

G

M
x
F

P

M

G

G

C

S

G

G

K

K

S

S

T

S

I

L

G

A

K

Q

I

E

L

L

S

G

K

L

E

A

L

L

N

K

M

L

K

E

F

V

Y

L

D

D

A

T

D

D

K

M

Y

I

L

I

E

S

D

E

R

R

N

V

N

G

M

L

K

S

I

V

S
x
R

D

E

M

I

F
|
F

K

E

R

E

-

L

G

G

E

E

E

D

F

N

F

F

R
|
R

N

M

L

F

E

E

S

K

A

N

A

L

L

I

K

D

E

E

L

L

-

K

S

T

F

L

E

K

E

T

N

P

S

H

V

V

I

I

A

S

T

T

G

G

G
|
G

A

I

V

V

T
x
M

R

H

E

E

K

N

N

-

I

-

L

-

C

-

L

L

K

K

K

G

K

L

G

G

K

T

I

T

V

F

F

Y

I

L

N

K

R

M

P

D

P

F

E

E

K

T

I

L

M

I

E

K

D

R

V

L

D

N

I

Q

E

K

-

E

-

R

-

A

N

K

R
|
R

P

P

L

L

E

-

H

N

G

N

K

L

S

T

R

Q

I

A

L

K

K

E

L

L

Y

F

E

E

E

K

R
|
R

Y

Q

K

A

L
|
L

Y
|
Y

I

E

N

K

Y

N

C

A

D

S

Y

F

Q

I

V

I

L

D

N

A

D

R

G

G

S

G

I

L

D

N

R

N

V

-

L

-

S

S

I

L

K

K

K

Q

Y

V

L

L

E

Q

binding 7-amino-4-hydroxy-3-[(E)-(5-hydroxy-7-sulfonaphthalen-2-yl)diazenyl]naphthalene-2-sulfonic acid: F9 (≠ M8), R45 (≠ S44), F48 (= F47), R57 (= R55), G80 (= G77), G81 (= G78), M84 (≠ T81), R116 (= R114), R132 (= R131), L135 (= L134), Y136 (= Y135)

6hqvA Pentafunctional arom complex from chaetomium thermophilum (see paper)
30% identity, 64% coverage: 2:107/165 of query aligns to 844:957/1555 of 6hqvA

query
sites
6hqvA

N

S

L

I

V

F

L

I

I

V

G

G

M

M

P

R

G

G

C

A

G

G

K

K

S

S

T

T

I

A

G

G

K

R

I

W

L

M

S

S

K

E

E

L

L

L

N

K

M

R

K

P

F

L

Y

V

D

D

A

L

D
|
D

K

A

Y

E

L

L

E

E

D

R

R

R

N

E

N

G

M

M

K

T

I

I

S

P

D

E

M

I

F

I

K

R

-

G

-

E

R

R

G

G

E

W

E

E

F

G

F

F

R

R

N

Q

L

A

E

E

S

L

A

E

A

L

L

L

K

Q

E

D

L

V

S

I

F

K

E

N

E

Q

N

S

S

K

-

G

-

Y

V

I

I

F

A

S

T

C

G

G

G
|
G

A

I

V

V

T

E

R

T

E

E

K

A

N

A

I

R

L

K

C

L

L

L

-

I

-

D

-

Y

-

H

K

K

K

N

K

G

G

G

K

P

I

V

V

L

F

L

I

V

N

H

R

R

P

D

P

T

E

D

K

Q

I

V

M

V

E

E

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D874 (= D32), G923 (= G77), G924 (= G78)

Sites not aligning to the query:

active site: 123, 145, 187, 243, 253, 257, 261, 264, 268, 280
binding (4S,5R)-4,5-dihydroxy-3-oxocyclohex-1-ene-1-carboxylic acid: 1060, 1062, 1181, 1224, 1232, 1242, 1243
binding glutamic acid: 139, 145, 187, 243, 257, 264, 280
binding nicotinamide-adenine-dinucleotide: 42, 44, 45, 76, 79, 107, 108, 109, 112, 132, 133, 135, 139, 140, 145, 154, 175, 176, 177, 180, 280
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 413, 562, 563, 979, 1277, 1279, 1323, 1327, 1348, 1368, 1526
binding zinc ion: 187, 264, 280

Query Sequence

>NP_347534.1 NCBI__GCF_000008765.1:NP_347534.1
MNLVLIGMPGCGKSTIGKILSKELNMKFYDADKYLEDRNNMKISDMFKRGEEFFRNLESA
ALKELSFEENSVIATGGGAVTREKNILCLKKKGKIVFINRPPEKIMEDVDIENRPLLEHG
KSRILKLYEERYKLYINYCDYQVLNDGSIDRVVLSIKKYLEGEGI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory