SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing NP_347574.1 NCBI__GCF_000008765.1:NP_347574.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O23346 Imidazoleglycerol-phosphate dehydratase 2, chloroplastic; IGPD 2; Protein HISTIDINE BIOSYNTHESIS 5B; EC 4.2.1.19 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
46% identity, 99% coverage: 1:195/197 of query aligns to 72:267/272 of O23346

query
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O23346

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E73 (= E2) mutation to Q: Loss of activity.
E86 (= E15) binding substrate
H112 (= H41) binding Mn(2+)
112:120 (vs. 41:49, 44% identical) binding substrate
H138 (= H67) binding Mn(2+)
HHTNE 138:142 (≠ HHTVE 67:71) binding substrate
H139 (= H68) binding Mn(2+)
E142 (= E71) binding Mn(2+)
R164 (= R93) binding substrate
R186 (= R115) binding substrate
H210 (≠ E139) binding Mn(2+)
H234 (= H163) binding Mn(2+)
234:242 (vs. 163:171, 67% identical) binding substrate
H235 (= H164) binding Mn(2+)
E238 (= E167) binding Mn(2+)
SSK 264:266 (≠ STK 192:194) binding substrate

6fwhA Acanthamoeba igpd in complex with r-c348 to 1.7a resolution (see paper)
48% identity, 98% coverage: 3:195/197 of query aligns to 1:193/196 of 6fwhA

query
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6fwhA

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binding [(2R)-2-hydroxy-3-(1H-1,2,4-triazol-1-yl)propyl]phosphonic acid: E13 (= E15), H39 (= H41), H65 (= H67), H66 (= H68), E69 (= E71), R91 (= R93), R113 (= R115), H161 (= H163), H162 (= H164), E165 (= E167), S190 (= S192), K192 (= K194)
binding manganese (ii) ion: H39 (= H41), H65 (= H67), E69 (= E71), H137 (≠ E139), H161 (= H163), E165 (= E167)

6fwhL Acanthamoeba igpd in complex with r-c348 to 1.7a resolution (see paper)
48% identity, 97% coverage: 4:195/197 of query aligns to 1:192/195 of 6fwhL

query
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6fwhL

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binding [(2R)-2-hydroxy-3-(1H-1,2,4-triazol-1-yl)propyl]phosphonic acid: E12 (= E15), H38 (= H41), H64 (= H67), H65 (= H68), E68 (= E71), R90 (= R93), R112 (= R115), H160 (= H163), H161 (= H164), E164 (= E167), S189 (= S192), K191 (= K194)
binding magnesium ion: Q5 (≠ F8), Q80 (≠ K83)
binding manganese (ii) ion: H38 (= H41), H64 (= H67), E68 (= E71), H136 (≠ E139), H160 (= H163), E164 (= E167)

5el9A A. Thaliana igpd2 in complex with the triazole-phosphonate inhibitor, (s)-c348, to 1.1a resolution (see paper)
46% identity, 97% coverage: 5:195/197 of query aligns to 3:194/199 of 5el9A

query
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5el9A

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binding [(2S)-2-hydroxy-3-(1H-1,2,4-triazol-1-yl)propyl]phosphonic acid: R91 (= R93), R113 (= R115), S191 (= S192), K193 (= K194)

5ekwA A. Thaliana igpd2 in complex with the racemate of the triazole- phosphonate inhibitor, c348 (see paper)
46% identity, 97% coverage: 5:195/197 of query aligns to 3:194/194 of 5ekwA

query
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5ekwA

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binding [(2S)-2-hydroxy-3-(1H-1,2,4-triazol-1-yl)propyl]phosphonic acid: R91 (= R93), R113 (= R115), S191 (= S192), K193 (= K194)
binding [(2R)-2-hydroxy-3-(1H-1,2,4-triazol-1-yl)propyl]phosphonic acid: R91 (= R93), R113 (= R115), S191 (= S192), K193 (= K194)

Q9S5G5 Histidine biosynthesis bifunctional protein HisB; EC 3.1.3.15; EC 4.2.1.19 from Escherichia coli O157:H7 (see paper)
48% identity, 98% coverage: 5:197/197 of query aligns to 167:355/355 of Q9S5G5

query
sites
Q9S5G5

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G

S

L

L

F

F

K

K

A

A

F

F

G

G

R

R

A

T

L

L

S

R

E

Q

A

A

V

I

S

-

K

-

N

-

T

-

R

R

I

V

K

E

G

G

-

D

-

T

V

L

M

P

S

S

T

S

K

K

G

G

S

V

I

L

Sites not aligning to the query:

1:166 Histidinol-phosphatase
9 binding Mg(2+)
11 binding Mg(2+)
17 E→A: Severe decrease in histidinol-phosphate phosphatase activity in presence of low magnesium concentration. At higher magnesium concentration (5mM), no effect on activity.
93 binding Zn(2+)
95 binding Zn(2+)
101 binding Zn(2+)
103 binding Zn(2+)
130 binding Mg(2+)

P34047 Imidazoleglycerol-phosphate dehydratase 1, chloroplastic; IGPD 1; Protein HISTIDINE BIOSYNTHESIS 5A; EC 4.2.1.19 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
45% identity, 99% coverage: 1:195/197 of query aligns to 70:265/270 of P34047

query
sites
P34047

M

M

E

A

E

L

K

G

R

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T

I

A

G

F

E

I

V

E

K

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R

K

V

T

T

T

K

E

E

T

T

S

N

I

V

E

S

V

V

D

K

I

I

N

N

L

L

D

D

G

G

E

T

G

G

K

V

Y

A

D

D

I

S

D

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T

S

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G

I

I

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F

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D

H
|
H

M

M

L

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A

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S

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H

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G

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G

D

D

L

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K

H

V

I

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D

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D

H
|
H

T

T

V

N

E
|
E

D

D

T

I

G

A

I

L

V

A

I

I

G

G

E

T

C

A

I

L

N

L

K

K

A

A

L

L

G

G

N

E

K

R

K

K

S

G

I

I

N

N

R

R

Y

F

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A

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P

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A

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I

Q

H

V

V

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S

M

L

D

D

I

L

S

S

G

G

R

R

A

P

F

Y

L

L

V

G

F

Y

D

N

G

L

E

E

F

I

T

P

C

T

E

Q

K

R

L

V

G

G

D

T

F

Y

Q

D

T

T

E

Q

M

L

V

V

E

E

E
x
H

F

F

R

Q

A

S

L

L

A

V

F

N

N

T

A

S

G

G

I

M

T

T

L

L

H

H

A

I

R

R

V

Q

I

L

Y

A

G

G

K

E

N

N

N

S

H
|
H

M

I

I

I

E
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E

G

A

L

T

F

F

K

K

A

A

F

F

G

A

R

R

A

A

L

L

S

R

E

Q

A

A

V

T

S

E

K

T

N

D

T

P

R

R

I

R

K

G

G

G

-

T

V

I

M

P

S

S

T

S

K

K

G

G

H110 (= H41) binding Mn(2+)
H136 (= H67) binding Mn(2+)
H137 (= H68) binding Mn(2+)
E140 (= E71) binding Mn(2+)
H208 (≠ E139) binding Mn(2+)
H232 (= H163) binding Mn(2+)
H233 (= H164) binding Mn(2+)
E236 (= E167) binding Mn(2+)

4qnkA The structure of wt a. Thaliana igpd2 in complex with mn2+ and phosphate (see paper)
47% identity, 92% coverage: 5:186/197 of query aligns to 3:184/185 of 4qnkA

query
sites
4qnkA

R

R

T

I

A

G

F

E

I

V

E

K

R

R

K

E

T

T

T

K

E

E

T

T

S

N

I

V

E

S

V

V

D

K

I

I

N

N

L

L

D

D

G

G

E

H

G

G

K

V

Y

S

D

D

I

S

D

S

T

T

G

G

I

I

G

P

F

F

F

L

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H
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H

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M

L

L

E

D

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x
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M

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H
|
H

G

G

L

L

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F

D

D

L

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K

H

V

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V

A

I

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L

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H
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H

H

H

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N

E
|
E

D

D

T

V

G

A

I

L

V

A

I

I

G

G

E

T

C

A

I

L

N

L

K

K

A

A

L

L

G

G

N

E

K

R

K

K

S

G

I

I

N

N

R

R

Y

F

G

G

T

D

S

F

F

T

V

A

P

P

M

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D

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E

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A

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M

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I

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S

G

G

R

R

A

P

F

Y

L

L

V

G

F

Y

D

N

G

L

E

E

F

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P

C

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E

Q

K

R

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V

G

G

D

T

F

Y

Q

D

T

T

E

Q

M

L

V

V

E

E

E
x
H

F

F

R

Q

A

S

L

L

A

V

F

N

N

T

A

S

G

G

I

M

T

T

L

L

H

H

A

I

R

R

V

Q

I

L

Y

A

G

G

K

K

N

N

N

S

H
|
H

H

H

M

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I

E
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E

G

A

L

T

F

F

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|
K

A

A

F

F

G

A

R

R

A

A

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L

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Q

A

A

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E

K

S

N

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R

R

binding manganese (ii) ion: H39 (= H41), H65 (= H67), E69 (= E71), H137 (≠ E139), H161 (= H163), E165 (= E167)
binding phosphate ion: Q43 (≠ L45), H47 (= H49), K169 (= K171)

4mu1A The structure of wt a. Thaliana igpd2 in complex with mn2+, imidazole, and sulfate at 1.5 a resolution (see paper)
47% identity, 92% coverage: 5:186/197 of query aligns to 3:184/185 of 4mu1A

query
sites
4mu1A

R

R

T

I

A

G

F

E

I

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E

K

R

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K

E

T

T

T

K

E

E

T

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S

N

I

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E

S

V

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K

I

I

N

N

L

L

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D

G

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H

G

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T

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|
H

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E

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D

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A

I

L

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A

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I

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C

A

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K

A

A

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G

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I

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N

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Y

L

L

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G

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D

N

G

L

E

E

F

I

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P

C

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E

Q

K

R

L

V

G

G

D

T

F

Y

Q

D

T

T

E

Q

M

L

V

V

E

E

E
x
H

F

F

R

Q

A

S

L

L

A

V

F

N

N

T

A

S

G

G

I

M

T

T

L

L

H

H

A

I

R

R

V

Q

I

L

Y

A

G

G

K

K

N

N

N

S

H

H

M

I

I

I

E

E

G

A

L

T

F

F

K

K

A

A

F

F

G

A

R

R

A

A

L

L

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R

E

Q

A

A

V

T

S

E

K

S

N

D

T

P

R

R

binding imidazole: H65 (= H67), E69 (= E71)
binding manganese (ii) ion: H65 (= H67), E69 (= E71), H137 (≠ E139)

4mu0A The structure of wt a. Thaliana igpd2 in complex with mn2+ and 1,2,4- triazole at 1.3 a resolution (see paper)
47% identity, 92% coverage: 5:186/197 of query aligns to 3:184/185 of 4mu0A

query
sites
4mu0A

R

R

T

I

A

G

F

E

I

V

E

K

R

R

K

E

T

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T

K

E

E

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S

N

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V

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N

L

L

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D

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G

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H

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K

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A

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L

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N

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F

Y

L

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N

G

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C

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Q

K

R

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G

G

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F

Y

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T

E

Q

M

L

V

V

E

E

E
x
H

F

F

R

Q

A

S

L

L

A

V

F

N

N

T

A

S

G

G

I

M

T

T

L

L

H

H

A

I

R

R

V

Q

I

L

Y

A

G

G

K

K

N

N

N

S

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H

M

I

I

I

E

E

G

A

L

T

F

F

K

K

A

A

F

F

G

A

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R

A

A

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R

E

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A

A

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E

K

S

N

D

T

P

R

R

binding manganese (ii) ion: H65 (= H67), E69 (= E71), H137 (≠ E139)
binding 1,2,4-triazole: H65 (= H67), H66 (= H68)

4mu3A The form a structure of an e21q catalytic mutant of a. Thaliana igpd2 in complex with mn2+ and a mixture of its substrate, 2r3s-igp, and an inhibitor, 2s3s-igp, to 1.12 a resolution (see paper)
46% identity, 92% coverage: 5:186/197 of query aligns to 3:184/186 of 4mu3A

query
sites
4mu3A

R

R

T

I

A

G

F

E

I

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E

K

R

R

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x
Q

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N

I

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E

S

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V

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K

I

I

N

N

L

L

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D

G

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G

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|
H

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E

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A

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A

I

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A

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K

A

A

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L

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G

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N

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D

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S

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G

R

R

A

P

F

Y

L

L

V

G

F

Y

D

N

G

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E

F

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C

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E

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K

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V

G

G

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T

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Y

Q

D

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T

E

Q

M

L

V

V

E

E

E
x
H

F

F

R

Q

A

S

L

L

A

V

F

N

N

T

A

S

G

G

I

M

T

T

L

L

H

H

A

I

R

R

V

Q

I

L

Y

A

G

G

K

K

N

N

N

S

H

H

M

I

I

I

E

E

G

A

L

T

F

F

K

K

A

A

F

F

G

A

R

R

A

A

L

L

S

R

E

Q

A

A

V

T

S

E

K

S

N

D

T

P

R

R

binding (2S,3S)-2,3-dihydroxy-3-(1H-imidazol-5-yl)propyl dihydrogen phosphate: H65 (= H67), H66 (= H68)
binding (2R,3S)-2,3-dihydroxy-3-(1H-imidazol-5-yl)propyl dihydrogen phosphate: Q13 (≠ E15), H65 (= H67), H66 (= H68)
binding manganese (ii) ion: H65 (= H67), E69 (= E71), H137 (≠ E139)

2f1dA X-ray structure of imidazoleglycerol-phosphate dehydratase (see paper)
46% identity, 92% coverage: 5:186/197 of query aligns to 2:183/183 of 2f1dA

query
sites
2f1dA

R

R

T

I

A

G

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E

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E

K

R

R

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T

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E

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I

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V

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D

K

I

I

N

N

L

L

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D

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G

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A

D

D

I

S

D

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S

G

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F

F

F

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D

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H
|
H

M

M

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M

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A

K

S

H

H

G

G

L

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G

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D

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H
|
H

H

H

T

T

V

N

E
|
E

D

D

T

I

G

A

I

L

V

A

I

I

G

G

E

T

C

A

I

L

N

L

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K

A

A

L

L

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G

N

E

K

R

K

K

S

G

I

I

N

N

R

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Y

F

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P

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F

Y

L

L

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F

Y

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N

G

L

E

E

F

I

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P

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E

Q

K

R

L

V

G

G

D

T

F

Y

Q

D

T

T

E

Q

M

L

V

V

E

E

E
x
H

F

F

R

Q

A

S

L

L

A

V

F

N

N

T

A

S

G

G

I

M

T

T

L

L

H

H

A

I

R

R

V

Q

I

L

Y

A

G

G

K

E

N

N

N

S

H
|
H

H

H

M

I

I

I

E
|
E

G

A

L

T

F

F

K

K

A

A

F

F

G

A

R

R

A

A

L

L

S

R

E

Q

A

A

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T

S

E

K

T

N

D

T

P

R

R

binding manganese (ii) ion: H38 (= H41), H64 (= H67), E68 (= E71), H136 (≠ E139), H160 (= H163), E164 (= E167)

8qawA Medicago truncatula hisn5 (igpd) in complex with mn, imd, edo, fmt, gol and trs (see paper)
46% identity, 92% coverage: 5:186/197 of query aligns to 3:184/185 of 8qawA

query
sites
8qawA

R

R

T

I

A

G

F

E

I

M

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K

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R

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T

T

T

K

E
|
E

T

T

S

N

I

V

E

S

V

V

D

K

I

I

N

N

L

L

D

D

G

G

E

T

G

G

K

V

Y

A

D

D

I

N

D

S

T

S

G

G

I

I

G

P

F

F

F

L

D

D

H
|
H

M

M

L

L

E

D

L
x
Q

M

L

S

A

K

S

H
|
H

G

G

L

L

I

F

D

D

L

V

K

H

V

V

K

K

V

A

I

T

G

G

D

D

L

T

K

H

V

I

D

D

S

D

H
|
H

T

T

V

N

E
|
E

D

D

T

V

G

A

I

L

V

A

I

I

G

G

E

T

C

A

I

L

N

L

K

Q

A

A

L

L

G

G

N

D

K

R

K

K

S

G

I

I

N

N

R

R

Y

F

G

G

T

N

S

F

F

S

V

A

P

P

M

L

D

D

E

E

S

A

L

L

C

V

Q

H

V

V

S

S

M

L

D

D

I

L

S

S

G

G

R

R

A

P

F

H

L

L

V

G

F

Y

D

D

G

L

E

N

F

I

T

P

C

T

E

Q

K

R

L

V

G

G

D

K

F

Y

Q

D

T

T

E

Q

M

L

V

V

E

E

E
x
H

F

F

R

Q

A

S

L

L

A

V

F

N

N

T

A

S

G

G

I

M

T

T

L

L

H

H

A

I

R

R

V

Q

I

F

Y

S

G

G

K

T

N

N

N

S

H
|
H

H

H

M

I

I

I

E
|
E

G

A

L

T

F

F

K
|
K

A

A

F

F

G

A

R

R

A

A

L

L

S

R

E

Q

A

A

V

T

S

E

K

Y

N

D

T

T

R

R

binding glycerol: Q43 (≠ L45), H47 (= H49), K169 (= K171)
binding imidazole: E13 (= E15), H66 (= H68), E69 (= E71)
binding manganese (ii) ion: H39 (= H41), H65 (= H67), E69 (= E71), H137 (≠ E139), H161 (= H163), E165 (= E167)

8qavA Medicago truncatula hisn5 (igpd) in complex with mn and ig2 (see paper)
46% identity, 92% coverage: 5:186/197 of query aligns to 2:183/184 of 8qavA

query
sites
8qavA

R

R

T

I

A

G

F

E

I

M

E

K

R

R

K

V

T

T

T

K

E

E

T

T

S

N

I

V

E

S

V

V

D

K

I

I

N

N

L

L

D

D

G

G

E

T

G

G

K

V

Y

A

D

D

I

N

D

S

T

S

G

G

I

I

G

P

F

F

F

L

D

D

H
|
H

M

M

L

L

E

D

L

Q

M

L

S

A

K

S

H

H

G

G

L

L

I

F

D

D

L

V

K

H

V

V

K

K

V

A

I

T

G

G

D

D

L

T

K

H

V

I

D

D

S

D

H
|
H

T

T

V

N

E
|
E

D

D

T

V

G

A

I

L

V

A

I

I

G

G

E

T

C

A

I

L

N

L

K

Q

A

A

L

L

G

G

N

D

K

R

K

K

S

G

I

I

N

N

R

R

Y

F

G

G

T

N

S

F

F

S

V

A

P

P

M

L

D

D

E

E

S

A

L

L

C

V

Q

H

V

V

S

S

M

L

D

D

I

L

S

S

G

G

R

R

A

P

F

H

L

L

V

G

F

Y

D

D

G

L

E

N

F

I

T

P

C

T

E

Q

K

R

L

V

G

G

D

K

F

Y

Q

D

T

T

E

Q

M

L

V

V

E

E

E
x
H

F

F

R

Q

A

S

L

L

A

V

F

N

N

T

A

S

G

G

I

M

T

T

L

L

H

H

A

I

R

R

V

Q

I

F

Y

S

G

G

K

T

N

N

N

S

H
|
H

H

H

M

I

I

I

E
|
E

G

A

L

T

F

F

K

K

A

A

F

F

G

A

R

R

A

A

L

L

S

R

E

Q

A

A

V

T

S

E

K

Y

N

D

T

T

R

R

binding (2S,3S)-2,3-dihydroxy-3-(1H-imidazol-5-yl)propyl dihydrogen phosphate: H64 (= H67), H65 (= H68)
binding manganese (ii) ion: H38 (= H41), H64 (= H67), E68 (= E71), H136 (≠ E139), H160 (= H163), E164 (= E167)

P9WML9 Imidazoleglycerol-phosphate dehydratase; IGPD; EC 4.2.1.19 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
41% identity, 93% coverage: 15:197/197 of query aligns to 21:210/210 of P9WML9

query
sites
P9WML9

E

E

T

S

S

D

I

I

E

V

V

I

D

E

I

L

N

D

L

L

D

D

G

G

E

T

G

G

K

Q

Y

V

D

A

I

V

D

D

T

T

G

G

I

V

G

P

F

F

F

Y

D

D

H
|
H

M
|
M

L
|
L

E
x
T

L
x
A

M
x
L

S
x
G

K
x
S

H
|
H

G

A

L

S

I

F

D

D

L

L

K

T

V

V

K

R

V

A

I

T

G

G

D

D

L

V

K

E

V

I

D

E

S

A

H
|
H

T
|
T

V
x
I

E
|
E

D

D

T

T

G

A

I

I

V

A

I

L

G

G

E

T

C

A

I

L

N

G

K

Q

A

A

L

L

G

G

N

D

K

K

K

R

S

G

I

I

N

R

R

R

Y

F

G

G

T

D

S

A

F

F

V

I

P

P

M

M

D

D

E

E

S

T

L

L

C

A

Q

H

V

A

S

A

M

V

D

D

I

L

S

S

G

G

R

R

A

P

F

Y

L

C

V

V

F

H

D

T

G

G

E

E

F

P

-

D

-

H

-

L

-

Q

-

H

-

T

T

I

C

A

E

G

K

S

L

S

G

V

D

P

F

Y

Q

H

T

T

E

V

M

I

V

N

E

R

E
x
H

F

V

F

F

R

E

A

S

L

L

A

A

F

A

N

N

A

A

G

R

I

I

T

A

L

L

H

H

A

V

R

R

V

V

I

L

Y

Y

G

G

K

R

N

D

N

P

H
|
H

M
x
I

I
x
T

E
|
E

G
x
A

L
x
Q

F
x
Y

K
|
K

A

A

F

V

G

A

R

R

A

A

L

L

S

R

E

Q

A

A

V

V

S

E

K

P

N

D

T

P

R

R

I

V

K

S

G

G

V

V

M

P

S

S

T

T

K

K

G

G

S

A

I

L

H47 (= H41) binding Mn(2+)
47:55 (vs. 41:49, 44% identical) binding substrate
H73 (= H67) binding Mn(2+)
HHTIE 73:77 (≠ HHTVE 67:71) binding substrate
H74 (= H68) binding Mn(2+)
E77 (= E71) binding Mn(2+)
H152 (≠ E139) binding Mn(2+)
H176 (= H163) binding Mn(2+)
176:184 (vs. 163:171, 44% identical) binding substrate
H177 (= H164) binding Mn(2+)
E180 (= E167) binding Mn(2+)

5xdsA Crystal structure of mycobacterium tuberculosis hisb bound with an inhibitor (see paper)
40% identity, 88% coverage: 15:187/197 of query aligns to 12:191/191 of 5xdsA

query
sites
5xdsA

E

E

T

S

S

D

I

I

E

V

V

I

D

E

I

L

N

D

L

L

D

D

G

G

E

T

G

G

K

Q

Y

V

D

A

I

V

D

D

T

T

G

G

I

V

G

P

F

F

F

Y

D

D

H
|
H

M

M

L

L

E

T

L

A

M

L

S

G

K

S

H

H

G

A

L

S

I

F

D

D

L

L

K

T

V

V

K

R

V

A

I

T

G

G

D

D

L

V

K

E

V

I

D

E

S

A

H
|
H

T

T

V

I

E
|
E

D

D

T

T

G

A

I

I

V

A

I

L

G

G

E

T

C

A

I

L

N

G

K

Q

A

A

L

L

G

G

N

D

K

K

K

R

S

G

I

I

N

R

R

R

Y

F

G

G

T

D

S

A

F

F

V

I

P

P

M

M

D

D

E

E

S

T

L

L

C

A

Q

H

V

A

S

A

M

V

D

D

I

L

S

S

G

G

R

R

A

P

F

Y

L

C

V

V

F

H

D

T

G

G

E

E

F

P

-

D

-

H

-

L

-

Q

-

H

-

T

T

I

C

A

E

G

K

S

L

S

G

V

D

P

F

Y

Q

H

T

T

E

V

M

I

V

N

E

R

E
x
H

F

V

F

F

R

E

A

S

L

L

A

A

F

A

N

N

A

A

G

R

I

I

T

A

L

L

H

H

A

V

R

R

V

V

I

L

Y

Y

G

G

K

R

N

D

N

P

H
|
H

H

H

M

I

I

T

E
|
E

G

A

L

Q

F

Y

K

K

A

A

F

V

G

A

R

R

A

A

L

L

S

R

E

Q

A

A

V

V

S

E

K

P

N

D

T

P

R

R

I

V

binding (2S)-2-azanyl-3-(4H-1,2,4-triazol-3-yl)propanoic acid: H64 (= H67), H65 (= H68), E68 (= E71)
binding manganese (ii) ion: H38 (= H41), H64 (= H67), E68 (= E71), H143 (≠ E139), H167 (= H163), E171 (= E167)

4lomA Crystal structure of mycobacterium tuberculosis hisb in complex with its substrate
40% identity, 88% coverage: 15:187/197 of query aligns to 12:191/191 of 4lomA

query
sites
4lomA

E

E

T

S

S

D

I

I

E

V

V

I

D

E

I

L

N

D

L

L

D

D

G

G

E

T

G

G

K

Q

Y

V

D

A

I

V

D

D

T

T

G

G

I

V

G

P

F

F

F

Y

D

D

H
|
H

M

M

L

L

E

T

L

A

M

L

S

G

K

S

H

H

G

A

L

S

I

F

D

D

L

L

K

T

V

V

K

R

V

A

I

T

G

G

D

D

L

V

K

E

V

I

D

E

S

A

H
|
H

H

H

T

T

V

I

E
|
E

D

D

T

T

G

A

I

I

V

A

I

L

G

G

E

T

C

A

I

L

N

G

K

Q

A

A

L

L

G

G

N

D

K

K

K

R

S

G

I

I

N

R

R

R

Y

F

G

G

T

D

S

A

F

F

V

I

P

P

M
|
M

D

D

E

E

S

T

L

L

C

A

Q

H

V

A

S

A

M

V

D

D

I

L

S

S

G

G

R

R

A

P

F

Y

L

C

V

V

F

H

D

T

G

G

E

E

F

P

-

D

-

H

-

L

-

Q

-

H

-

T

T

I

C

A

E

G

K

S

L

S

G

V

D

P

F

Y

Q

H

T

T

E

V

M

I

V

N

E

R

E
x
H

F

V

F

F

R

E

A

S

L

L

A

A

F

A

N

N

A

A

G

R

I

I

T

A

L

L

H

H

A

V

R

R

V

V

I

L

Y

Y

G

G

K

R

N

D

N

P

H
|
H

M

I

I

T

E
|
E

G

A

L

Q

F

Y

K
|
K

A

A

F

V

G

A

R

R

A

A

L

L

S

R

E

Q

A

A

V

V

S

E

K

P

N

D

T

P

R

R

I

V

binding (2R,3S)-2,3-dihydroxy-3-(1H-imidazol-5-yl)propyl dihydrogen phosphate: H38 (= H41), M98 (= M101), H167 (= H163), H168 (= H164), E171 (= E167), K175 (= K171)
binding manganese (ii) ion: H38 (= H41), H64 (= H67), E68 (= E71), H143 (≠ E139), H167 (= H163), E171 (= E167)

4gquA Crystal structure of hisb from mycobacterium tuberculosis
40% identity, 88% coverage: 15:187/197 of query aligns to 12:191/191 of 4gquA

query
sites
4gquA

E

E

T

S

S

D

I

I

E

V

V

I

D

E

I

L

N

D

L

L

D

D

G

G

E

T

G

G

K

Q

Y

V

D

A

I

V

D

D

T

T

G

G

I

V

G

P

F

F

F

Y

D

D

H
|
H

M

M

L

L

E

T

L

A

M

L

S

G

K

S

H

H

G

A

L

S

I

F

D

D

L

L

K

T

V

V

K

R

V

A

I

T

G

G

D

D

L

V

K

E

V

I

D

E

S

A

H
|
H

H

H

T

T

V

I

E
|
E

D

D

T

T

G

A

I

I

V

A

I

L

G

G

E

T

C

A

I

L

N

G

K

Q

A

A

L

L

G

G

N

D

K

K

K

R

S

G

I

I

N

R

R

R

Y

F

G

G

T

D

S

A

F

F

V

I

P

P

M

M

D

D

E

E

S

T

L

L

C

A

Q

H

V

A

S

A

M

V

D

D

I

L

S

S

G

G

R

R

A

P

F

Y

L

C

V

V

F

H

D

T

G

G

E

E

F

P

-

D

-

H

-

L

-

Q

-

H

-

T

T

I

C

A

E

G

K

S

L

S

G

V

D

P

F

Y

Q

H

T

T

E

V

M

I

V

N

E

R

E
x
H

F

V

F

F

R

E

A

S

L

L

A

A

F

A

N

N

A

A

G

R

I

I

T

A

L

L

H

H

A

V

R

R

V

V

I

L

Y

Y

G

G

K

R

N

D

N

P

H
|
H

H

H

M

I

I

T

E
|
E

G

A

L

Q

F

Y

K

K

A

A

F

V

G

A

R

R

A

A

L

L

S

R

E

Q

A

A

V

V

S

E

K

P

N

D

T

P

R

R

I

V

binding manganese (ii) ion: H38 (= H41), H64 (= H67), E68 (= E71), H143 (≠ E139), H167 (= H163), E171 (= E167)

7fcyA Crystal structure of m.Tuberculosis imidazole glycerol phosphate dehydratase in complex with an inhibitor
40% identity, 87% coverage: 15:186/197 of query aligns to 12:190/190 of 7fcyA

query
sites
7fcyA

E

E

T

S

S

D

I

I

E

V

V

I

D

E

I

L

N

D

L

L

D

D

G

G

E

T

G

G

K

Q

Y

V

D

A

I

V

D

D

T

T

G

G

I

V

G

P

F

F

F

Y

D

D

H
|
H

M

M

L

L

E

T

L

A

M

L

S

G

K

S

H

H

G

A

L

S

I

F

D

D

L

L

K

T

V

V

K

R

V

A

I

T

G

G

D

D

L

V

K

E

V

I

D

E

S

A

H
|
H

H

H

T

T

V

I

E
|
E

D

D

T

T

G

A

I

I

V

A

I

L

G

G

E

T

C

A

I

L

N

G

K

Q

A

A

L

L

G

G

N

D

K

K

K

R

S

G

I

I

N

R

R

R

Y

F

G

G

T

D

S

A

F

F

V

I

P

P

M
|
M

D

D

E

E

S

T

L

L

C

A

Q

H

V

A

S

A

M

V

D

D

I

L

S

S

G

G

R

R

A

P

F

Y

L

C

V

V

F

H

D

T

G

G

E

E

F

P

-

D

-

H

-

L

-

Q

-

H

-

T

T

I

C

A

E

G

K

S

L

S

G

V

D

P

F

Y

Q

H

T

T

E

V

M

I

V

N

E

R

E
x
H

F

V

F

F

R

E

A

S

L

L

A

A

F

A

N

N

A

A

G

R

I

I

T

A

L

L

H

H

A

V

R

R

V

V

I

L

Y

Y

G

G

K

R

N

D

N

P

H
|
H

M

I

I

T

E
|
E

G

A

L

Q

F

Y

K

K

A

A

F

V

G

A

R

R

A

A

L

L

S

R

E

Q

A

A

V

V

S

E

K

P

N

D

T

P

R

R

binding (1S)-2-methyl-1-(2-methyl-1,2,4-triazol-3-yl)propan-1-amine: H38 (= H41), M98 (= M101), H167 (= H163), H168 (= H164), E171 (= E167)
binding manganese (ii) ion: H38 (= H41), H64 (= H67), E68 (= E71), H143 (≠ E139), H167 (= H163), E171 (= E167)

7dnqA Crystal structure of m.Tuberculosis imidazole glycerol phosphate dehydratase in complex with an inhibitor
40% identity, 87% coverage: 15:186/197 of query aligns to 12:190/190 of 7dnqA

query
sites
7dnqA

E

E

T

S

S

D

I

I

E

V

V

I

D

E

I

L

N

D

L

L

D

D

G

G

E

T

G

G

K

Q

Y

V

D

A

I

V

D

D

T

T

G

G

I

V

G

P

F

F

F

Y

D

D

H
|
H

M

M

L

L

E

T

L

A

M

L

S

G

K

S

H

H

G

A

L

S

I

F

D

D

L

L

K

T

V

V

K

R

V

A

I

T

G

G

D

D

L

V

K

E

V

I

D

E

S

A

H
|
H

T

T

V

I

E
|
E

D

D

T

T

G

A

I

I

V

A

I

L

G

G

E

T

C

A

I

L

N

G

K

Q

A

A

L

L

G

G

N

D

K

K

K

R

S

G

I

I

N

R

R

R

Y

F

G

G

T

D

S

A

F

F

V

I

P

P

M

M

D

D

E

E

S

T

L

L

C

A

Q

H

V

A

S

A

M

V

D

D

I

L

S

S

G

G

R

R

A

P

F

Y

L

C

V

V

F

H

D

T

G

G

E

E

F

P

-

D

-

H

-

L

-

Q

-

H

-

T

T

I

C

A

E

G

K

S

L

S

G

V

D

P

F

Y

Q

H

T

T

E

V

M

I

V

N

E

R

E
x
H

F

V

F

F

R

E

A

S

L

L

A

A

F

A

N

N

A

A

G

R

I

I

T

A

L

L

H

H

A

V

R

R

V

V

I

L

Y

Y

G

G

K

R

N

D

N

P

H
|
H

H

H

M

I

I

T

E
|
E

G

A

L

Q

F

Y

K

K

A

A

F

V

G

A

R

R

A

A

L

L

S

R

E

Q

A

A

V

V

S

E

K

P

N

D

T

P

R

R

binding 2-(1H-1,2,4-triazol-5-ylsulfanyl)ethanamine: H64 (= H67), H65 (= H68), E68 (= E71)
binding manganese (ii) ion: H38 (= H41), H64 (= H67), E68 (= E71), H143 (≠ E139), H167 (= H163), E171 (= E167)

Query Sequence

>NP_347574.1 NCBI__GCF_000008765.1:NP_347574.1
MEEKRTAFIERKTTETSIEVDINLDGEGKYDIDTGIGFFDHMLELMSKHGLIDLKVKVIG
DLKVDSHHTVEDTGIVIGECINKALGNKKSINRYGTSFVPMDESLCQVSMDISGRAFLVF
DGEFTCEKLGDFQTEMVEEFFRALAFNAGITLHARVIYGKNNHHMIEGLFKAFGRALSEA
VSKNTRIKGVMSTKGSI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory