SitesBLAST
Comparing NP_661098.1 NCBI__GCF_000006985.1:NP_661098.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6hulB Sulfolobus solfataricus tryptophan synthase ab complex (see paper)
46% identity, 90% coverage: 15:427/458 of query aligns to 4:414/416 of 6hulB
- binding pyridoxal-5'-phosphate: H103 (= H112), K104 (= K113), Q131 (= Q140), S220 (= S230), G255 (= G265), G256 (= G266), G257 (= G267), S258 (= S268), N259 (= N269), E371 (= E384), S400 (= S413), G401 (= G414)
- binding serine: K104 (= K113), T127 (= T136), G128 (= G137), A129 (= A138), G130 (= G139), W132 (= W141)
4qysB Trpb2 enzymes (see paper)
45% identity, 91% coverage: 7:423/458 of query aligns to 3:412/418 of 4qysB
- active site: K101 (= K113), E123 (= E135), S402 (= S413)
- binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: H100 (= H112), K101 (= K113), G252 (= G265), G253 (= G266), G254 (= G267), S255 (= S268), N256 (= N269), E373 (= E384), S402 (= S413)
4qysA Trpb2 enzymes (see paper)
43% identity, 91% coverage: 7:423/458 of query aligns to 3:402/408 of 4qysA
- active site: K108 (= K113), E130 (= E135), S392 (= S413)
- binding pyridoxal-5'-phosphate: H107 (= H112), K108 (= K113), G242 (= G265), G243 (= G266), G244 (= G267), S245 (= S268), N246 (= N269), E363 (= E384), S392 (= S413)
- binding phosphoserine: K108 (= K113), G132 (= G137), A133 (= A138), Q135 (= Q140), W136 (= W141), G316 (= G337), R318 (= R339), E363 (= E384)
5ey5B Lbcats
35% identity, 75% coverage: 77:421/458 of query aligns to 47:379/383 of 5ey5B
- binding pyridoxal-5'-phosphate: H81 (= H112), K82 (= K113), Q109 (= Q140), S185 (= S230), G227 (= G265), G229 (= G267), S230 (= S268), N231 (= N269), E345 (= E384), S371 (= S413), G372 (= G414)
5vm5D Engineered tryptophan synthase b-subunit from pyrococcus furiosus, pftrpb2b9, with ser bound (see paper)
33% identity, 80% coverage: 54:420/458 of query aligns to 19:376/383 of 5vm5D
- active site: K82 (= K113), E104 (= E135), S369 (= S413)
- binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: H81 (= H112), K82 (= K113), T105 (= T136), G106 (= G137), A107 (= A138), Q109 (= Q140), H110 (≠ W141), S185 (= S230), G227 (= G265), G229 (= G267), S230 (= S268), N231 (= N269), G296 (= G337), E343 (= E384), S369 (= S413)
5t6mA Structure of the tryptophan synthase b-subunit from pyroccus furiosus with b-methyltryptophan non-covalently bound (see paper)
33% identity, 80% coverage: 54:420/458 of query aligns to 19:376/383 of 5t6mA
5ixjD Tryptophan synthase beta-subunit from pyrococcus furiosus with l- threonine non-covalently bound in the active site (see paper)
33% identity, 80% coverage: 54:420/458 of query aligns to 19:376/394 of 5ixjD
5dw3A Tryptophan synthase beta-subunit from pyrococcus furiosus with product l-tryptophan non-covalently bound in the active site (see paper)
33% identity, 80% coverage: 54:420/458 of query aligns to 19:377/383 of 5dw3A
- active site: K82 (= K113), E104 (= E135), S370 (= S413)
- binding tryptophan: K82 (= K113), E104 (= E135), T105 (= T136), G106 (= G137), A107 (= A138), Q109 (= Q140), H110 (≠ W141), S185 (= S230), G228 (= G266), Y300 (= Y340)
7rnpA Engineered tryptophan synthase b-subunit from pyrococcus furiosus, pftrpb2b9_h275e with 4-cl-trp non-covalently bound (see paper)
33% identity, 80% coverage: 54:420/458 of query aligns to 19:378/384 of 7rnpA
6am8B Engineered tryptophan synthase b-subunit from pyrococcus furiosus, pftrpb2b9 with trp bound as e(aex2) (see paper)
33% identity, 80% coverage: 54:420/458 of query aligns to 19:378/385 of 6am8B
- active site: K82 (= K113), E104 (= E135), S371 (= S413)
- binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-l-tryptophane: H81 (= H112), K82 (= K113), E104 (= E135), T105 (= T136), G106 (= G137), A107 (= A138), Q109 (= Q140), H110 (≠ W141), L161 (= L197), S185 (= S230), V187 (≠ L232), G227 (= G265), G228 (= G266), G229 (= G267), S230 (= S268), N231 (= N269), G298 (= G337), Y301 (= Y340), E345 (= E384), S371 (= S413), G372 (= G414)
- binding tryptophan: L169 (≠ I215), S274 (≠ M315), H275 (≠ T316)
Sites not aligning to the query:
5t6mB Structure of the tryptophan synthase b-subunit from pyroccus furiosus with b-methyltryptophan non-covalently bound (see paper)
33% identity, 80% coverage: 54:420/458 of query aligns to 19:378/386 of 5t6mB
1v8zA X-ray crystal structure of the tryptophan synthase b2 subunit from hyperthermophile, pyrococcus furiosus (see paper)
33% identity, 80% coverage: 54:420/458 of query aligns to 19:378/386 of 1v8zA
- active site: K82 (= K113), E104 (= E135), S371 (= S413)
- binding pyridoxal-5'-phosphate: H81 (= H112), K82 (= K113), Q109 (= Q140), S185 (= S230), G227 (= G265), G228 (= G266), G229 (= G267), S230 (= S268), N231 (= N269), E345 (= E384), S371 (= S413), G372 (= G414)
5dw0A Trpb from pyrococcus furiosus with l-serine bound as the external aldimine (see paper)
33% identity, 80% coverage: 54:420/458 of query aligns to 19:378/388 of 5dw0A
- active site: K82 (= K113), E104 (= E135), S371 (= S413)
- binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: H81 (= H112), K82 (= K113), T105 (= T136), G106 (= G137), A107 (= A138), Q109 (= Q140), H110 (≠ W141), S185 (= S230), G227 (= G265), G229 (= G267), S230 (= S268), N231 (= N269), G298 (= G337), D300 (≠ R339), E345 (= E384), S371 (= S413)
6cuzA Engineered trpb from pyrococcus furiosus, pftrpb7e6 with (2s,3r)- ethylserine bound as the amino-acrylate (see paper)
32% identity, 80% coverage: 54:420/458 of query aligns to 19:378/383 of 6cuzA
- binding (2E)-2-[(E)-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)amino]pent-2-enoic acid: H81 (= H112), K82 (= K113), T105 (= T136), G106 (= G137), A107 (= A138), Q109 (= Q140), H110 (≠ W141), S185 (= S230), G227 (= G265), G229 (= G267), S230 (= S268), N231 (= N269), G298 (= G337), E345 (= E384), S371 (= S413)
6cutA Engineered holo trpb from pyrococcus furiosus, pftrpb7e6 with (2s,3s)- isopropylserine bound as the external aldimine (see paper)
32% identity, 80% coverage: 54:420/458 of query aligns to 19:378/385 of 6cutA
- binding (2S,3S)-3-hydroxy-2-[(E)-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)amino]-4-methylpentanoic acid (non-preferred name): H81 (= H112), K82 (= K113), T105 (= T136), G106 (= G137), A107 (= A138), Q109 (= Q140), H110 (≠ W141), S185 (= S230), G227 (= G265), G229 (= G267), S230 (= S268), N231 (= N269), G298 (= G337), E345 (= E384), S371 (= S413)
5ocwB Structure of mycobacterium tuberculosis tryptophan synthase in space group f222 (see paper)
33% identity, 75% coverage: 79:421/458 of query aligns to 60:390/399 of 5ocwB
- active site: K93 (= K113), E115 (= E135), S382 (= S413)
- binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: H92 (= H112), K93 (= K113), T116 (= T136), G117 (= G137), A118 (= A138), Q120 (= Q140), H121 (≠ W141), T196 (≠ S230), G238 (= G265), G240 (= G267), S241 (= S268), N242 (= N269), G309 (= G337), E356 (= E384), S382 (= S413)
6uapB Crystal structure of tryptophan synthase from m. Tuberculosis - open form with brd6309 bound
33% identity, 75% coverage: 79:421/458 of query aligns to 64:394/405 of 6uapB
- active site: K97 (= K113), E119 (= E135), S386 (= S413)
- binding (2R,3S,4R)-3-(4'-chloro-2',6'-difluoro[1,1'-biphenyl]-4-yl)-4-(fluoromethyl)azetidine-2-carbonitrile: I180 (= I211), N181 (≠ S212), F184 (≠ I215), Y196 (= Y226), F198 (≠ L228), P204 (vs. gap), F207 (vs. gap), H290 (≠ R302)
6u6cB Crystal structure of tryptophan synthase from m. Tuberculosis - aminoacrylate- and gsk2-bound form (see paper)
33% identity, 75% coverage: 79:421/458 of query aligns to 65:395/405 of 6u6cB
- active site: K98 (= K113), E120 (= E135), S387 (= S413)
- binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: H97 (= H112), K98 (= K113), T121 (= T136), G122 (= G137), A123 (= A138), Q125 (= Q140), H126 (≠ W141), T201 (≠ S230), G243 (= G265), G245 (= G267), S246 (= S268), N247 (= N269), G314 (= G337), E361 (= E384), S387 (= S413)
- binding 1-(2-fluorobenzene-1-carbonyl)-N-methyl-2,3-dihydro-1H-indole-5-sulfonamide: F185 (≠ I215), W188 (≠ A218), Y197 (= Y226), F199 (≠ L228), G204 (vs. gap), P205 (vs. gap), H291 (≠ R302), G292 (≠ A303)
Sites not aligning to the query:
6usaB Crystal structure of tryptophan synthase from m. Tuberculosis - aminoacrylate- and gsk1-bound form (see paper)
33% identity, 75% coverage: 79:421/458 of query aligns to 64:394/404 of 6usaB
- active site: K97 (= K113), E119 (= E135), S386 (= S413)
- binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: H96 (= H112), K97 (= K113), T120 (= T136), G121 (= G137), A122 (= A138), G123 (= G139), Q124 (= Q140), H125 (≠ W141), T200 (≠ S230), G242 (= G265), G244 (= G267), S245 (= S268), N246 (= N269), G313 (= G337), E360 (= E384), S386 (= S413)
- binding (3R,4R)-4-[4-(2-Chlorophenyl)piperazin-1-yl]-1,1-dioxothiolan-3-ol: F184 (≠ I215), W187 (≠ A218), Y196 (= Y226), F198 (≠ L228), G203 (vs. gap), P204 (vs. gap), F207 (vs. gap), H290 (≠ R302), G291 (≠ A303)
6dweB Crystal structure of tryptophan synthase from m. Tuberculosis - aminoacrylate- and brd0059-bound form
33% identity, 75% coverage: 79:421/458 of query aligns to 64:394/404 of 6dweB
- active site: K97 (= K113), E119 (= E135), S386 (= S413)
- binding (2R,3S,4R)-3-(2',6'-difluoro-4'-methyl[1,1'-biphenyl]-4-yl)-4-(fluoromethyl)azetidine-2-carbonitrile: F184 (≠ I215), Y196 (= Y226), F198 (≠ L228), P204 (vs. gap), F207 (vs. gap), H290 (≠ R302)
- binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: H96 (= H112), K97 (= K113), T120 (= T136), G121 (= G137), A122 (= A138), G123 (= G139), Q124 (= Q140), H125 (≠ W141), T200 (≠ S230), G242 (= G265), G244 (= G267), S245 (= S268), N246 (= N269), G313 (= G337), E360 (= E384), S386 (= S413)
Query Sequence
>NP_661098.1 NCBI__GCF_000006985.1:NP_661098.1
MSTEPTKILLSEDEMPRQWYNIQADLPSPMPPPVGLDGNPIGPDALAKVFPMNLIEQEVS
TERWIDIPEEILGILKLWRPSPLYRARRLEAALGTPAKIYYKNEGVSPAGSHKPNTAVAQ
AWYNREFGIKYLTTETGAGQWGSALAMSCKLIGIECKVFMVRISFDQKPFRKIMMNTWGA
ECIPSPSPLTAVGRRILEEDPDTPGSLGIAISEAIEQAVERDDTRYALGSVLNHVMLHQT
IIGLEARKQFDKIGRYPDIVIGCAGGGSNFAGISFPFLYDKIHGKDVQVIATEPEACPTL
TRAPYAYDSGDVAMMTPLLPMHSLGHTFIPPAIHAGGLRYHGMAPLVSHTKQLGLIEATA
LPQTECYEAALLFAHTEGFIPAPETSHAIAQTIREAKQAKEEGKEKVILMNWSGHGLMDL
QGYDAYMSGKISDYPLPEELLQRSIAASLEGHPPVPGC
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory