SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing NP_661517.1 NCBI__GCF_000006985.1:NP_661517.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 10 hits to proteins with known functional sites (download)

A0QUX7 Acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS; EC 2.2.1.6 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
45% identity, 97% coverage: 3:157/159 of query aligns to 8:162/170 of A0QUX7

query
sites
A0QUX7

H

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K46 (≠ P41) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

2pc6A Crystal structure of putative acetolactate synthase- small subunit from nitrosomonas europaea (see paper)
44% identity, 99% coverage: 1:158/159 of query aligns to 2:159/164 of 2pc6A

query
sites
2pc6A

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binding calcium ion: N66 (= N65), L68 (= L67), V71 (≠ T70)

Q9FFF4 Acetolactate synthase small subunit 2, chloroplastic; ALS-interacting protein 3; Acetohydroxy-acid synthase small subunit 2; Protein valine-tolerant 1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
44% identity, 97% coverage: 2:156/159 of query aligns to 76:228/477 of Q9FFF4

query
sites
Q9FFF4

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G88 (= G14) mutation to D: Resistance to valine ihibition.
T119 (= T47) mutation to I: In vat1-1; resistance to valine ihibition.

Sites not aligning to the query:

453 R→Q: In vat1-3; resistance to valine ihibition.

P9WKJ3 Putative acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS; EC 2.2.1.6 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
44% identity, 97% coverage: 3:157/159 of query aligns to 6:160/168 of P9WKJ3

query
sites
P9WKJ3

H

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K44 (≠ P41) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

6vz8F Arabidopsis thaliana acetohydroxyacid synthase complex with valine bound (see paper)
39% identity, 97% coverage: 2:156/159 of query aligns to 4:156/159 of 6vz8F

query
sites
6vz8F

K

K

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binding valine: D13 (≠ N11), E14 (≠ K12), G16 (= G14), M17 (≠ T15), I18 (≠ L16), N31 (= N29), I32 (≠ L30)

Q93YZ7 Acetolactate synthase small subunit 1, chloroplastic; ALS-interacting protein 1; Acetohydroxyacid synthase small subunit 1 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
39% identity, 97% coverage: 2:156/159 of query aligns to 86:238/491 of Q93YZ7

query
sites
Q93YZ7

K

K

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D95 (≠ N11) binding
G98 (= G14) mutation to E: Increased feedback inhibition by valine/isoleucine.
M99 (≠ T15) binding
I100 (≠ L16) binding
F107 (= F23) mutation to A: Decreased feedback inhibition by leucine and valine/isoleucine.
Y112 (≠ F28) mutation to A: Increased feedback inhibition by leucine.
N113 (= N29) binding ; mutation to A: Decreased feedback inhibition by valine/isoleucine.
I114 (≠ L30) binding

Sites not aligning to the query:

328 binding
331 G→D: Decreased feedback inhibition by leucine and valine/isoleucine.
332 binding
333 binding
340 F→A: Decreased feedback inhibition by valine/isoleucine.
345 Y→A: No effect.
346 binding ; mutation N->A,H: Decreased feedback inhibition by leucine and increased feedback inhibition by valine/isoleucine.
347 binding

6vz8G Arabidopsis thaliana acetohydroxyacid synthase complex with valine bound (see paper)
38% identity, 97% coverage: 3:156/159 of query aligns to 4:157/159 of 6vz8G

query
sites
6vz8G

H

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binding valine: D12 (≠ N11), G15 (= G14), V16 (≠ T15), L17 (= L16), N30 (= N29), I31 (≠ L30), V36 (≠ I35)

O60086 Probable acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
30% identity, 98% coverage: 2:157/159 of query aligns to 70:265/289 of O60086

query
sites
O60086

K

R

H

H

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N

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C

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F

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E

E

D

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P

E

E

N

I

L

S

S

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M

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T

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I

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L

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G

E

A

D

D

R

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I

V

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E

Q

Q

V

A

L

K

K

R

Q

Q

L

I

N

E

R

D

L

I

I

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S

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V

I

W

K

A

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Y

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L

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A

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K

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N

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N

L

A

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A

L

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L

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K

A

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Q

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G

A

Sites not aligning to the query:

34 modified: Phosphoserine

6wo1B Hybrid acetohydroxyacid synthase complex structure with cryptococcus neoformans ahas catalytic subunit and saccharomyces cerevisiae ahas regulatory subunit (see paper)
33% identity, 97% coverage: 2:156/159 of query aligns to 4:195/197 of 6wo1B

query
sites
6wo1B

K

Q

H

H

L

V

I

L

S

N

V

C

L

L

V

V

E

Q

N

N

K
x
E

F

P

G
|
G

T

V

L
|
L

N

S

R

R

V

V

A

S

A

G

M

T

F

L

S

A

A

A

R

R

G

G

F

F

N

N

L

I

E

D

S

S

I

L

S

V

I

V

G

C

E

N

T

T

E

E

D

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K

E

D

I

L

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S

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M

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Y

K

A

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Y

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L

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E

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H

Q

L

H

N

E

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I

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L

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N

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F

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R

binding valine: E14 (≠ K12), G16 (= G14), L18 (= L16)

6u9dL Saccharomyces cerevisiae acetohydroxyacid synthase (see paper)
29% identity, 97% coverage: 2:156/159 of query aligns to 37:240/255 of 6u9dL

query
sites
6u9dL

K

Q

H

H

L

V

I

L

S

N

V

C

L

L

V

V

E

Q

N

N

K

E

F

P

G

G

T

V

L

L

N

S

R

R

V

V

A

S

A

G

M

T

F

L

S

A

A

A

R

R

G

G

F

F

N

N

L

I

E

D

S

S

I

L

S

V

I

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G

C

E

N

T

T

E

E

D

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K

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M

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L

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Y

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A

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Y

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L

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N

E

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L

L

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N

N

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x
N

F
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F

K

G

G

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R

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V

D
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D

I
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I

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x
K

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K
x
R

I

I

N

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T

A

A

F

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L

D

K

L

L

F

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P

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G

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A

A

R

R

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S

G

G

A

M

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M

A

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R
|
R

binding adenosine-5'-triphosphate: N191 (≠ V107), F192 (= F108), V197 (= V113), D198 (= D114), I199 (= I115), K211 (≠ S127), R214 (≠ K130), R214 (≠ K130), R240 (= R156)

Query Sequence

>NP_661517.1 NCBI__GCF_000006985.1:NP_661517.1
MKHLISVLVENKFGTLNRVAAMFSARGFNLESISIGETEDPEISRMTIVTRGEDRIISQV
LKQLNRLIDTIKVTDLTHQPHVERELLLLSLKLSKSTQHEIFELANVFKGKVVDIKQKSI
TIEFVGSPDKINTAIDLFRPFGIRELARSGAVAIHRGES

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory