SitesBLAST
Comparing NP_662486.1 NCBI__GCF_000006985.1:NP_662486.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1mtoA Crystal structure of a phosphofructokinase mutant from bacillus stearothermophilus bound with fructose-6-phosphate (see paper)
28% identity, 94% coverage: 1:375/401 of query aligns to 1:317/319 of 1mtoA
- active site: G11 (= G11), R72 (= R60), C73 (≠ L61), D103 (= D110), G104 (≠ D111), G124 (≠ K131), T125 (= T132), D127 (= D134), D129 (= D136), R171 (= R176)
- binding 6-O-phosphono-beta-D-fructofuranose: D127 (= D134), R162 (= R167), M169 (≠ F174), R171 (= R176), E222 (= E234), R243 (= R301), H249 (= H307), R252 (= R310)
6pfkA Phosphofructokinase, inhibited t-state (see paper)
28% identity, 94% coverage: 1:375/401 of query aligns to 1:317/319 of 6pfkA
- active site: G11 (= G11), R72 (= R60), C73 (≠ L61), D103 (= D110), G104 (≠ D111), G124 (≠ K131), T125 (= T132), D127 (= D134), D129 (= D136), R171 (= R176)
- binding 2-phosphoglycolic acid: R21 (≠ K21), R25 (≠ L25), G58 (vs. gap), D59 (≠ N46), R154 (≠ K159), R211 (≠ Q220), K213 (≠ D222)
P00512 ATP-dependent 6-phosphofructokinase; ATP-PFK; Phosphofructokinase; Phosphohexokinase; EC 2.7.1.11 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see 2 papers)
28% identity, 94% coverage: 1:375/401 of query aligns to 1:317/319 of P00512
- RSVVR 21:25 (≠ KGAAL 21:25) binding ADP
- D59 (≠ N46) binding ADP
- RC 72:73 (≠ RL 60:61) binding ATP
- D103 (= D110) binding Mg(2+)
- TID 125:127 (≠ TMD 132:134) binding in other chain
- R154 (≠ K159) binding in other chain
- R162 (= R167) binding substrate
- MGR 169:171 (≠ FGR 174:176) binding in other chain
- GAE 185:187 (≠ EAD 190:192) binding in other chain
- R211 (≠ Q220) binding in other chain
- KKH 213:215 (≠ DVH 222:224) binding in other chain
- E222 (= E234) binding in other chain
- R243 (= R301) binding substrate
- HVQR 249:252 (≠ HLVR 307:310) binding in other chain
1pfkA Crystal structure of the complex of phosphofructokinase from escherichia coli with its reaction products (see paper)
29% identity, 85% coverage: 1:339/401 of query aligns to 2:282/320 of 1pfkA
- active site: G12 (= G11), R73 (vs. gap), F74 (vs. gap), D104 (= D110), G105 (≠ D111), G125 (≠ K131), T126 (= T132), D128 (= D134), D130 (= D136), R172 (= R176)
- binding adenosine-5'-diphosphate: G11 (= G10), R22 (≠ K21), R26 (≠ L25), Y56 (≠ A59), S59 (≠ D62), D60 (≠ Q63), R73 (vs. gap), F74 (vs. gap), F77 (≠ A73), R78 (≠ G74), G103 (= G109), D104 (= D110), G105 (≠ D111), S106 (= S112), M108 (≠ S114), G109 (≠ V115), R155 (≠ K159), G213 (= G235), K214 (≠ M236), H216 (≠ N238)
- binding 1,6-di-O-phosphono-beta-D-fructofuranose: G12 (= G11), R73 (vs. gap), T126 (= T132), D128 (= D134), M170 (≠ F174), E223 (≠ D246), H250 (= H307), R253 (= R310)
- binding magnesium ion: G186 (≠ E190), E188 (≠ D192)
P0A796 ATP-dependent 6-phosphofructokinase isozyme 1; ATP-PFK 1; Phosphofructokinase 1; 6-phosphofructokinase isozyme I; Phosphohexokinase 1; Sedoheptulose-7-phosphate kinase; EC 2.7.1.11 from Escherichia coli (strain K12) (see 2 papers)
29% identity, 85% coverage: 1:339/401 of query aligns to 2:282/320 of P0A796
- G12 (= G11) binding ATP
- RGVVR 22:26 (≠ KGAAL 21:25) binding ADP
- RYSVSD 55:60 (≠ EARLDQ 58:63) binding ADP
- RF 73:74 (vs. gap) binding ATP
- GDGS 103:106 (≠ GDDS 109:112) binding ATP
- D104 (= D110) binding Mg(2+)
- TID 126:128 (≠ TMD 132:134) binding in other chain
- D128 (= D134) active site, Proton acceptor; mutation to S: 18000-fold reduction of catalytic rate.
- R155 (≠ K159) binding in other chain
- R163 (= R167) binding substrate
- R172 (= R176) mutation to S: 3.4-fold reduction in turnover numbers.
- GCE 186:188 (≠ EAD 190:192) binding in other chain
- KKH 214:216 (≠ MKN 236:238) binding in other chain
- E223 (≠ D246) binding in other chain
- R244 (= R301) binding substrate
- HIQR 250:253 (≠ HLVR 307:310) binding in other chain
4pfkA Phosphofructokinase. Structure and control (see paper)
28% identity, 94% coverage: 1:375/401 of query aligns to 1:317/319 of 4pfkA
- active site: G11 (= G11), R72 (= R60), C73 (≠ L61), D103 (= D110), G104 (≠ D111), G124 (≠ K131), T125 (= T132), D127 (= D134), D129 (= D136), R171 (= R176)
- binding adenosine-5'-diphosphate: S9 (= S9), Y41 (= Y41), R72 (= R60), C73 (≠ L61), F76 (= F64), K77 (≠ Q65), G104 (≠ D111), G108 (≠ V115), R154 (≠ K159), G185 (≠ E190), R211 (≠ Q220), G212 (≠ S221), K213 (≠ D222), H215 (= H224)
- binding 6-O-phosphono-beta-D-fructofuranose: D127 (= D134), M169 (≠ F174), E222 (= E234), H249 (= H307), R252 (= R310)
- binding magnesium ion: G185 (≠ E190), E187 (≠ D192)
3pfkA Phosphofructokinase. Structure and control (see paper)
28% identity, 94% coverage: 1:375/401 of query aligns to 1:317/319 of 3pfkA
- active site: G11 (= G11), R72 (= R60), C73 (≠ L61), D103 (= D110), G104 (≠ D111), G124 (≠ K131), T125 (= T132), D127 (= D134), D129 (= D136), R171 (= R176)
- binding phosphate ion: R154 (≠ K159), K213 (≠ D222), H249 (= H307), R252 (= R310)
4i4iA Crystal structure of bacillus stearothermophilus phosphofructokinase mutant t156a bound to pep (see paper)
27% identity, 94% coverage: 1:375/401 of query aligns to 1:317/319 of 4i4iA
- active site: G11 (= G11), R72 (= R60), C73 (≠ L61), D103 (= D110), G104 (≠ D111), G124 (≠ K131), T125 (= T132), D127 (= D134), D129 (= D136), R171 (= R176)
- binding phosphoenolpyruvate: R21 (≠ K21), R25 (≠ L25), G58 (vs. gap), R154 (≠ K159), R211 (≠ Q220), K213 (≠ D222), H215 (= H224)
5xz6A Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adenylylimidodiphosphate, the atp analogue (see paper)
30% identity, 60% coverage: 1:239/401 of query aligns to 1:229/318 of 5xz6A
5xzaA Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adp (see paper)
30% identity, 60% coverage: 1:239/401 of query aligns to 1:229/322 of 5xzaA
5xz7A Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adenylylimidodiphosphate, the atp analogue (see paper)
30% identity, 60% coverage: 1:239/401 of query aligns to 1:229/322 of 5xz7A
Sites not aligning to the query:
Q2FXM8 ATP-dependent 6-phosphofructokinase; ATP-PFK; Phosphofructokinase; Phosphohexokinase; EC 2.7.1.11 from Staphylococcus aureus (strain NCTC 8325 / PS 47) (see paper)
30% identity, 60% coverage: 1:239/401 of query aligns to 1:229/322 of Q2FXM8
- RC 72:73 (≠ AG 69:70) binding ATP
- GDGS 102:105 (≠ GDDS 109:112) binding ATP
- TID 127:129 (≠ TMD 132:134) binding in other chain
- G150 (≠ Q153) mutation to D: Exhibits higher affinity for fructose 6-phosphate and higher catalytic activity with a loss of dimer conversion; in association with A-151.
- L151 (≠ F154) mutation to A: Exhibits higher affinity for fructose 6-phosphate and higher catalytic activity with a loss of tetramer-dimer conversion; in association with D-150.
- R164 (= R167) mutation to A: Complete loss of fructose 6-phosphate binding.
- MGR 171:173 (≠ FGR 174:176) binding in other chain
- E224 (= E234) binding in other chain
Sites not aligning to the query:
- 245 R→A: Complete loss of fructose 6-phosphate binding.
- 251:254 binding in other chain
5xz9A Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adenylylimidodiphosphate, the atp analogue (see paper)
30% identity, 60% coverage: 1:239/401 of query aligns to 1:229/321 of 5xz9A
- binding adenosine-5'-triphosphate: S9 (= S9), Y41 (= Y41), C73 (≠ G70), F76 (≠ A73), K77 (≠ G74), G102 (= G109), D103 (= D110), G104 (≠ D111), S105 (= S112), R107 (vs. gap), G108 (= G113)
3o8lA Structure of phosphofructokinase from rabbit skeletal muscle (see paper)
25% identity, 84% coverage: 2:336/401 of query aligns to 8:319/748 of 3o8lA
- active site: G17 (= G11), R80 (= R77), D111 (= D110), S156 (≠ T132), D158 (= D134), D160 (= D136), R202 (= R176)
- binding adenosine-5'-diphosphate: D165 (≠ T139), M166 (≠ Y140), F300 (≠ Y317)
- binding adenosine-5'-triphosphate: G16 (= G10), Y47 (= Y41), R80 (= R77), C81 (≠ V78), R85 (≠ P86), G110 (= G109), G112 (≠ D111), S113 (= S112), T115 (vs. gap), G116 (vs. gap), W219 (≠ C193), H234 (= H209), R238 (= R213)
Sites not aligning to the query:
P08237 ATP-dependent 6-phosphofructokinase, muscle type; ATP-PFK; PFK-M; 6-phosphofructokinase type A; Phosphofructo-1-kinase isozyme A; PFK-A; Phosphohexokinase; EC 2.7.1.11 from Homo sapiens (Human) (see 6 papers)
25% identity, 84% coverage: 2:336/401 of query aligns to 16:327/780 of P08237
- R39 (≠ L25) to P: in GSD7; Italian; dbSNP:rs121918193
- G57 (= G43) to V: in GSD7; Italian
- R100 (≠ K81) to Q: in dbSNP:rs2228500
- S180 (= S146) to C: in GSD7; Italian
- G209 (= G175) to D: in GSD7; loss of activity shown by complementation assays in yeast; dbSNP:rs767265360
- D309 (= D318) to G: in GSD7; Spanish; complete loss of enzyme activity; dbSNP:rs1169383137
Sites not aligning to the query:
- 543 D → A: in GSD7; Italian; dbSNP:rs121918194
- 557 modified: N6-(2-hydroxyisobutyryl)lysine
- 591 D → A: in GSD7; Italian
- 686 W → C: in GSD7; Japanese; dbSNP:rs121918196
- 696 R → H: in dbSNP:rs41291971
P00511 ATP-dependent 6-phosphofructokinase, muscle type; ATP-PFK; PFK-M; 6-phosphofructokinase type A; Phosphofructo-1-kinase isozyme A; PFK-A; Phosphohexokinase; EC 2.7.1.11 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
25% identity, 84% coverage: 2:336/401 of query aligns to 16:327/780 of P00511
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylthreonine
- 775 modified: Phosphoserine; by PKA
7lw1A Human phosphofructokinase-1 liver type bound to activator na-11 (see paper)
25% identity, 83% coverage: 2:335/401 of query aligns to 6:316/744 of 7lw1A
- binding adenosine-5'-diphosphate: R78 (≠ K79), C79 (≠ I80), T83 (≠ S84), G108 (= G109), G110 (≠ D111), S111 (= S112), G114 (vs. gap), I117 (vs. gap)
- binding 6-O-phosphono-beta-D-fructofuranose: I155 (vs. gap), D156 (vs. gap), R191 (= R167), M198 (≠ F174), R200 (= R176), E254 (= E234), R282 (= R279), H288 (= H307), R291 (= R310)
- binding N-{(11S)-2-[2-(5-hydroxypent-1-yn-1-yl)phenyl]-4H,10H-pyrazolo[5,1-c][1,4]benzoxazepin-7-yl}acetamide: M164 (vs. gap), F298 (≠ Y317), I301 (≠ N320), K305 (≠ E324)
Sites not aligning to the query:
- binding 1,6-di-O-phosphono-beta-D-fructofuranose: 399, 460, 519, 555, 562, 618, 644, 650, 724
- binding N-{(11S)-2-[2-(5-hydroxypent-1-yn-1-yl)phenyl]-4H,10H-pyrazolo[5,1-c][1,4]benzoxazepin-7-yl}acetamide: 331, 527, 532, 535, 568, 660, 730, 734
P17858 ATP-dependent 6-phosphofructokinase, liver type; ATP-PFK; PFK-L; 6-phosphofructokinase type B; Phosphofructo-1-kinase isozyme B; PFK-B; Phosphohexokinase; EC 2.7.1.11 from Homo sapiens (Human) (see 3 papers)
25% identity, 83% coverage: 2:335/401 of query aligns to 16:326/780 of P17858
- G81 (≠ A66) natural variant: G -> A
- R151 (≠ Q138) to W: in dbSNP:rs755851304
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 527 T→A: Does not affect GlcNAcylation.
- 529 modified: carbohydrate, O-linked (GlcNAc) serine; S→A: Prevents GlcNAcylation and enhance enzyme activity.
4xz2A Human platelet phosphofructokinase in an r-state in complex with adp and f6p, crystal form i (see paper)
26% identity, 83% coverage: 4:335/401 of query aligns to 3:287/708 of 4xz2A
Sites not aligning to the query:
- binding 1,6-di-O-phosphono-beta-D-fructofuranose: 372, 433, 491, 492, 535, 591, 623, 626
- binding phosphate ion: 382, 386, 419, 420
Q01813 ATP-dependent 6-phosphofructokinase, platelet type; ATP-PFK; PFK-P; 6-phosphofructokinase type C; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphohexokinase; EC 2.7.1.11 from Homo sapiens (Human) (see paper)
25% identity, 83% coverage: 2:335/401 of query aligns to 25:335/784 of Q01813
Sites not aligning to the query:
- 386 modified: Phosphoserine; S→A: Decreased interaction with ATG4B.
Query Sequence
>NP_662486.1 NCBI__GCF_000006985.1:NP_662486.1
MKKIGILTSGGDCGGLNAVIKGAALMALNKGLELYVIPNGYAGLYNLLNQDRLVRLDEAR
LDQFQASFAGSEAGHSRVKIKAISNPDKYNRIKVGMKKFDLDALIISGGDDSGSVMIDLN
HNGIQCIHCPKTMDLDLQTYSVGGDSTINRIAQFVEDLKTTGRTHNRVLVTEVFGRYAGH
TAFRSGVAAEADCILIPEIPVDWDVVYEHIVERFTRRIRQSDVHSGTYTIVVAEGMKNAD
GTDIVDESAGIDAFGHKKLAGAGKYVCQEIKKRFKTDPRMPQFMKDTGMFVEGIYEIPEV
REVHPGHLVRAGHSSAYDINFGFEAGAAAVLLLLEGKTGVTISKVKGRKIEYIEASKAIE
QRYVDLDQVAMYESIGTCFGRTPAAYEPILREVDGVYERIY
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory