SitesBLAST
Comparing NP_662653.1 NCBI__GCF_000006985.1:NP_662653.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3m4rA Structure of the n-terminal class ii aldolase domain of a conserved protein from thermoplasma acidophilum
39% identity, 32% coverage: 13:234/694 of query aligns to 13:212/213 of 3m4rA
8ijgC Crystal structure of alcohol dehydrogenase m5 from burkholderia gladioli with NADP
37% identity, 26% coverage: 445:627/694 of query aligns to 6:185/250 of 8ijgC
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G452), T15 (≠ A454), S16 (≠ G455), I18 (= I457), R38 (≠ I477), R39 (≠ D478), D60 (= D499), V61 (= V500), N87 (= N526), G89 (= G528), R110 (≠ L549), N135 (= N577), A137 (≠ S579), Y150 (= Y592), K154 (= K596), P180 (≠ A622), G181 (≠ D623), T183 (≠ I625), T185 (≠ S627)
Sites not aligning to the query:
3ai2A The crystal structure of l-sorbose reductase from gluconobacter frateurii complexed with NADPH (see paper)
32% identity, 35% coverage: 443:687/694 of query aligns to 5:258/263 of 3ai2A
- active site: G18 (≠ A456), S144 (= S579), Y157 (= Y592), K161 (= K596)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G14 (= G452), S16 (≠ A454), S17 (≠ G455), G18 (≠ A456), I19 (= I457), A38 (≠ D476), R39 (≠ I477), Q40 (≠ D478), V64 (≠ C498), D65 (= D499), V66 (= V500), N92 (= N526), G94 (= G528), N142 (= N577), Y157 (= Y592), K161 (= K596), P187 (≠ A622), I190 (= I625), T192 (= T631), W195 (≠ M634)
6zyzA Structure of the borneol dehydrogenases of salvia rosmarinus with NAD+ (see paper)
32% identity, 35% coverage: 445:687/694 of query aligns to 5:245/259 of 6zyzA
- binding nicotinamide-adenine-dinucleotide: G12 (= G452), S15 (≠ G455), G16 (≠ A456), I17 (= I457), D36 (= D476), I37 (= I477), Q38 (≠ D478), C58 (= C498), D59 (= D499), V60 (= V500), N86 (= N526), A87 (≠ V527), V90 (≠ A530), I110 (≠ L549), T137 (≠ N577), Y152 (= Y592), K156 (= K596), V185 (≠ I625)
- binding (2S)-1-[3-{[(2R)-2-hydroxypropyl]oxy}-2,2-bis({[(2R)-2-hydroxypropyl]oxy}methyl)propoxy]propan-2-ol: P93 (≠ Q532), N94 (≠ G533), S95 (≠ R534), D98 (= D537)
7krmC Putative fabg bound to nadh from acinetobacter baumannii
32% identity, 35% coverage: 443:687/694 of query aligns to 3:240/244 of 7krmC
- active site: G18 (≠ A456), S140 (≠ N577), Y155 (= Y592)
- binding nicotinamide-adenine-dinucleotide: G12 (= G452), S15 (≠ G455), G18 (≠ A456), I19 (= I457), D38 (= D476), L39 (≠ I477), A60 (≠ C498), N61 (≠ D499), V62 (= V500), N88 (= N526), V111 (≠ L549), S140 (≠ N577), Y155 (= Y592), K159 (= K596), I188 (≠ L630), T190 (≠ P632)
3ai3C The crystal structure of l-sorbose reductase from gluconobacter frateurii complexed with NADPH and l-sorbose (see paper)
32% identity, 35% coverage: 443:687/694 of query aligns to 5:258/263 of 3ai3C
- active site: G18 (≠ A456), S144 (= S579), Y157 (= Y592), K161 (= K596)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G14 (= G452), S16 (≠ A454), S17 (≠ G455), G18 (≠ A456), I19 (= I457), A38 (≠ D476), R39 (≠ I477), Q40 (≠ D478), R43 (≠ A481), D65 (= D499), V66 (= V500), N92 (= N526), G94 (= G528), N142 (= N577), Y157 (= Y592), K161 (= K596), P187 (≠ A622), G188 (≠ D623), I190 (= I625), T192 (= T631), W195 (≠ M634)
- binding alpha-L-sorbopyranose: A252 (≠ I681), F254 (≠ T683)
- binding L-sorbose: G96 (≠ A530), E154 (≠ F589), Y157 (= Y592), W195 (≠ M634)
Sites not aligning to the query:
3ai3A The crystal structure of l-sorbose reductase from gluconobacter frateurii complexed with NADPH and l-sorbose (see paper)
32% identity, 35% coverage: 443:687/694 of query aligns to 5:258/263 of 3ai3A
- active site: G18 (≠ A456), S144 (= S579), Y157 (= Y592), K161 (= K596)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G14 (= G452), S16 (≠ A454), S17 (≠ G455), G18 (≠ A456), I19 (= I457), A38 (≠ D476), R39 (≠ I477), Q40 (≠ D478), R43 (≠ A481), V64 (≠ C498), D65 (= D499), V66 (= V500), N92 (= N526), G94 (= G528), N142 (= N577), S144 (= S579), Y157 (= Y592), K161 (= K596), P187 (≠ A622), G188 (≠ D623), I190 (= I625), T192 (= T631), W195 (≠ M634)
- binding L-sorbose: G96 (≠ A530), S144 (= S579), L151 (≠ G586), E154 (≠ F589), Y157 (= Y592), G188 (≠ D623)
F1SWA0 Zerumbone synthase; EC 1.1.1.326 from Zingiber zerumbet (Shampoo ginger) (Amomum zerumbet) (see paper)
31% identity, 35% coverage: 445:687/694 of query aligns to 5:255/267 of F1SWA0
- S142 (≠ N577) mutation to A: Strong reduction in oxidoreductase activity toward 8-hydroxy-alpha-humulene and borneol.
- S144 (= S579) mutation to A: Increased oxidoreductase activity toward 8-hydroxy-alpha-humulene and borneol.
- Y155 (= Y592) mutation to A: Strong reduction in oxidoreductase activity toward 8-hydroxy-alpha-humulene and borneol.
- K159 (= K596) mutation to A: Abolishes all oxidoreductase activity.
4wecA Crystal structure of a short chain dehydrogenase from mycobacterium smegmatis
32% identity, 35% coverage: 443:687/694 of query aligns to 8:249/258 of 4wecA
- active site: G21 (≠ A456), S143 (≠ V578), Q154 (≠ F589), Y157 (= Y592), K161 (= K596)
- binding nicotinamide-adenine-dinucleotide: G17 (= G452), A19 (= A454), S20 (≠ G455), G21 (≠ A456), I22 (= I457), D41 (= D476), I42 (= I477), V61 (≠ C498), D62 (= D499), V63 (= V500), N89 (= N526), T141 (≠ Y576), Y157 (= Y592), K161 (= K596), P187 (≠ A622), P189 (≠ R624), V190 (≠ I625)
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
28% identity, 35% coverage: 446:690/694 of query aligns to 8:254/255 of 5itvA
- active site: G18 (≠ A456), S141 (= S579), Y154 (= Y592), K158 (= K596)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G452), S17 (≠ G455), G18 (≠ A456), I19 (= I457), D38 (= D476), I39 (= I477), T61 (≠ C498), I63 (≠ V500), N89 (= N526), G91 (= G528), T139 (≠ N577), S141 (= S579), Y154 (= Y592), K158 (= K596), P184 (≠ A622), G185 (≠ D623), I186 (≠ R624), I187 (= I625)
A0A3Q8GL18 (+)-cis,trans-nepetalactol synthase NEPS1; Nepetalactol-related short-chain dehydrogenase; Nepetalactol dehydrogenase; Nepetalactol-related short-chain reductase 1; Nepetalactol-related SDR1; NmNEPS1; EC 5.5.1.34; EC 1.1.1.419 from Nepeta racemosa (Catmint) (Raceme catnip) (see paper)
28% identity, 35% coverage: 442:687/694 of query aligns to 14:261/271 of A0A3Q8GL18
- N125 (= N550) mutation to A: Strongly reduced cis-trans-nepetalactone levels.
- T152 (≠ N577) mutation to N: Absence of cis-trans-nepetalactone.
- T153 (≠ V578) mutation to A: Almost normal cis-trans-nepetalactone levels.
- T154 (≠ S579) mutation to G: Loss of dehydrogenase activity and strongly enhanced cis-trans-nepetalactol levels associated with a huge increase in Km for cis-trans-nepetalactol.
- P155 (≠ K580) mutation to S: Strongly reduced cis-trans-nepetalactone levels.
- L156 (≠ Q581) mutation to S: Reduced dehydrogenase activity and absence of cis-trans-nepetalactone.
- Y167 (= Y592) mutation to F: Absence of cis-trans-nepetalactone.
- K171 (= K596) mutation to M: Absence of cis-trans-nepetalactone.
- S198 (≠ D623) mutation to M: Absence of cis-trans-nepetalactone.
- V199 (≠ R624) mutation to A: Almost normal cis-trans-nepetalactone levels.
- T202 (vs. gap) mutation to A: Absence of cis-trans-nepetalactone.
2ewmB Crystal structure of the (s)-specific 1-phenylethanol dehydrogenase of the denitrifying bacterium strain ebn1 (see paper)
33% identity, 26% coverage: 446:627/694 of query aligns to 6:187/247 of 2ewmB
- active site: G16 (≠ A456), S139 (vs. gap), Y149 (≠ F589), Y152 (= Y592), K156 (= K596)
- binding nicotinamide-adenine-dinucleotide: G12 (= G452), N15 (≠ G455), G16 (≠ A456), I17 (= I457), D36 (= D476), L37 (≠ I477), C59 (= C498), D60 (= D499), V61 (= V500), N87 (= N526), S139 (vs. gap), Y152 (= Y592), K156 (= K596), P182 (≠ A622), S183 (≠ D623), L184 (≠ R624), V185 (≠ I625)
Sites not aligning to the query:
Q5P5I4 (S)-1-Phenylethanol dehydrogenase; EC 1.1.1.311 from Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) (see 2 papers)
33% identity, 26% coverage: 446:627/694 of query aligns to 8:189/249 of Q5P5I4
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 191 binding NAD(+)
1zemA Crystal structure of NAD+-bound xylitol dehydrogenase (see paper)
31% identity, 29% coverage: 442:639/694 of query aligns to 2:202/260 of 1zemA
- active site: N16 (≠ A456), S142 (≠ G569), Y155 (= Y592), K159 (= K596)
- binding nicotinamide-adenine-dinucleotide: G12 (= G452), G15 (= G455), N16 (≠ A456), I17 (= I457), D36 (= D476), M37 (≠ I477), D62 (= D499), V63 (= V500), N89 (= N526), A90 (≠ V527), G91 (= G528), T140 (≠ R567), S142 (≠ G569), Y155 (= Y592), K159 (= K596), P185 (≠ A622), M188 (≠ I625)
Sites not aligning to the query:
3ak4A Crystal structure of nadh-dependent quinuclidinone reductase from agrobacterium tumefaciens
29% identity, 35% coverage: 442:687/694 of query aligns to 4:254/258 of 3ak4A
- active site: G18 (≠ A456), S141 (= S579), L151 (≠ F589), Y154 (= Y592), K158 (= K596), E199 (≠ R638)
- binding nicotinamide-adenine-dinucleotide: K17 (≠ G455), G18 (≠ A456), I19 (= I457), D38 (= D476), L39 (≠ I477), V60 (≠ C498), D61 (= D499), V62 (= V500), N88 (= N526), A89 (≠ V527), G90 (= G528), T139 (≠ N577), S141 (= S579), Y154 (= Y592), K158 (= K596), G185 (≠ D623), V187 (≠ I625), T189 (≠ S627), M191 (≠ L629)
6f9qC Binary complex of a 7s-cis-cis-nepetalactol cyclase from nepeta mussinii with NAD+ (see paper)
29% identity, 35% coverage: 442:687/694 of query aligns to 5:253/260 of 6f9qC
- active site: G19 (≠ A456), Y159 (= Y592)
- binding nicotinamide-adenine-dinucleotide: G15 (= G452), S18 (≠ G455), G19 (≠ A456), I20 (= I457), D40 (= D476), I41 (= I477), Q42 (≠ D478), C63 (= C498), D64 (= D499), V65 (= V500), N91 (= N526), A92 (≠ V527), G93 (= G528), N144 (= N577), Y159 (= Y592), K163 (= K596), P189 (≠ A622), M190 (≠ D623), V192 (≠ I625), T194 (≠ S627), P195 (≠ G628), L196 (= L629), T197 (≠ S639)
A0A3Q8GLE8 (+)-cis,cis-nepetalactol synthase NEPS3; Nepetalactol-related short-chain reductase 3; NmNEPS3; EC 5.5.1.35 from Nepeta racemosa (Catmint) (Raceme catnip) (see paper)
29% identity, 35% coverage: 442:687/694 of query aligns to 11:259/270 of A0A3Q8GLE8
- 21:27 (vs. 452:458, 71% identical) binding NAD(+)
- DIQ 46:48 (≠ DID 476:478) binding NAD(+)
- DV 70:71 (= DV 499:500) binding NAD(+)
- N97 (= N526) binding NAD(+)
- N150 (= N577) mutation to T: No effect on catalytic activity.
- S154 (≠ Q581) mutation to L: Abolishes catalytic activity.
- YVMSK 165:169 (≠ YGLPK 592:596) binding NAD(+)
- K169 (= K596) mutation to M: Abolishes catalytic activity.
- M196 (≠ D623) mutation to S: Abolishes catalytic activity.
- VATPL 198:202 (≠ IRSGL 625:629) binding NAD(+)
7qujA Structure of nsneps2, a 7s-cis-trans nepetalactone synthase (see paper)
31% identity, 35% coverage: 442:687/694 of query aligns to 3:254/259 of 7qujA
- binding nicotinamide-adenine-dinucleotide: G13 (= G452), S16 (≠ G455), G17 (≠ A456), I18 (= I457), D38 (= D476), I39 (= I477), Q40 (≠ D478), C61 (= C498), D62 (= D499), I63 (≠ V500), N89 (= N526), A90 (≠ V527), G91 (= G528), T145 (≠ N577), S146 (≠ V578), Y160 (= Y592), K164 (= K596), P190 (≠ A622), M191 (≠ D623), A192 (≠ R624), V193 (≠ I625), T195 (vs. gap), L197 (vs. gap), T198 (vs. gap)
6zzsD Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and 3-oxovalerate (see paper)
27% identity, 35% coverage: 445:690/694 of query aligns to 7:260/261 of 6zzsD
- active site: G18 (≠ A456), S143 (≠ A582), Y156 (= Y592)
- binding nicotinamide-adenine-dinucleotide: G14 (= G452), S17 (≠ G455), I19 (= I457), D38 (= D476), M39 (≠ I477), D64 (= D499), V65 (= V500), N91 (= N526), A92 (≠ V527), G93 (= G528), M141 (≠ K580), A142 (≠ Q581), S143 (≠ A582), Y156 (= Y592), K160 (= K596), P186 (≠ A622), G187 (≠ D623), V189 (≠ I625), T191 (≠ S627), L193 (= L629)
- binding 3-oxidanylidenepentanoic acid: Q95 (≠ A530), S143 (≠ A582), N145 (= N584), K153 (vs. gap), Y156 (= Y592), Q197 (≠ E633)
6zzqA Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and acetoacetate (see paper)
27% identity, 35% coverage: 445:690/694 of query aligns to 6:259/260 of 6zzqA
- active site: G17 (≠ A456), S142 (≠ A582), Y155 (= Y592)
- binding acetoacetic acid: Q94 (≠ A530), S142 (≠ A582), K152 (vs. gap), Y155 (= Y592), Q196 (≠ E633)
- binding nicotinamide-adenine-dinucleotide: G13 (= G452), S16 (≠ G455), G17 (≠ A456), I18 (= I457), D37 (= D476), M38 (≠ I477), D63 (= D499), V64 (= V500), N90 (= N526), A91 (≠ V527), G92 (= G528), M140 (≠ K580), A141 (≠ Q581), S142 (≠ A582), Y155 (= Y592), K159 (= K596), Y187 (≠ R624), V188 (≠ I625), T190 (≠ S627)
Query Sequence
>NP_662653.1 NCBI__GCF_000006985.1:NP_662653.1
MEAGRIDAADTPAELVELVYASRLLGKDDRLVMHGGGNTSVKCELTDFIGNHVNVIFIKA
SGVNLASVDAGDFTPVRIDPLRKLQKMYESGQRHSEEDIRRFSTREFKNFLYLNLFTLTD
HMVSRSLSPSIETLLHAFLPHRYILHTHSLALLTLSNQTDGERLCREALGDGYGQVPYIQ
PGLGLANLAHDAYEKNPSIEGLVLHKHGLVTFGDSAKEAYDRMIDGVNRIEERIASAARK
VFASAPMPTAIASVEEVAPIVRGACSFEKTPGEKDYQSFVLEFRTSPALLDYLKIADLEA
FSKKGAMTPDFIIRTKNHPLVAPAPDAADLEGFGKELRARAKRFTEEYRSYFERQQQATG
MDVSMIDPMPRVVLVPGLGLFGLGLSAADAKLTADIAEHSAVAMLDAESIGCFESISEKE
AFEIEYWDMEQAKVRKSHNGGEFAGKVALVTGGAGAIGLATAKAFKAKGAEIVIMDIDPA
ALEKAAAELGSGTLTIPCDVTNAAAVREAFDTVCRTFGGLDIVVSNVGVAWQGRIGDVSD
ELLRRSFELNFFSHQTVSQNAVRIMRRQGIGGVLLYNVSKQAVNPGPDFGPYGLPKAATL
FLLRQYALDHGRDGIRANGVNADRIRSGLLTPEMIKARSAARGLSERDYMAGNLLGLEVS
AEDVADAFVHLALETKTTGSITTVDGGNIAAALR
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory