SitesBLAST

D10 (= D10) mutation to N: Does not affect H-NS binding ability.
R16 (= R16) mutation to C: Derepression of the hly operon, impaired H-NS binding.
D22 (= D22) mutation to N: Does not affect H-NS binding ability.
E25 (= E25) mutation to Q: Affects H-NS binding ability. Decrease in the ability to repress the expression of the hly operon.
E29 (= E29) mutation to Q: Does not affect H-NS binding ability.
E34 (= E34) mutation to Q: Does not affect H-NS binding ability.
D37 (= D37) mutation to N: Does not affect H-NS binding ability.
E39 (= E39) mutation to Q: Does not affect H-NS binding ability.
44:72 (vs. 44:72, 100% identical) mutation Missing: Derepression of the hly operon, impaired H-NS binding.
D48 (= D48) mutation D->E,R: Abolishes the interaction with H-NS.; mutation to N: Abolishes the interaction with H-NS. Loss of the ability to repress the expression of the hly operon.
R50 (= R50) mutation to H: Derepression of the hly operon, impaired H-NS binding.
58:72 (vs. 58:72, 100% identical) mutation Missing: Derepression of the hly operon, impaired H-NS binding.
D61 (= D61) mutation to N: Does not affect H-NS binding ability.
P64 (= P64) mutation P->L,S: Derepression of the hly operon, impaired H-NS binding.
R72 (= R72) mutation to RHHHHHH: Derepression of the hly operon, impaired H-NS binding.

Q7CR17 Hemolysin expression-modulating protein Hha from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
99% identity, 100% coverage: 1:72/72 of query aligns to 1:72/72 of Q7CR17

query
sites
Q7CR17

M

M

S

S

E

D

K

K

P

P

L

L

T

T

K

K

T

T

D

D

Y

Y

L

L

M

M

R
|
R

L
|
L

R
|
R

C

C

Q

Q

T

T

I

I

D

D

T

T

L

L

E

E

R
|
R

V

V

I

I

E

E

K

K

N

N

K

K

Y

Y

E

E

L

L

S

S

D

D

N

N

E

E

L

L

A

A

V

V

F

F

Y

Y

S

S

A

A

D
|
D

H

H

R

R

L

L

A

A

E

E

L

L

T

T

M

M

N

N

K

K

L

L

Y

Y

D

D

K

K

I

I

P

P

S

S

V

V

W

W

K

K

F

F

I

I

R

R

R14 (= R14) mutation to A: Still interacts with H-NS, derepresses expression of H-NS/Hha coregulated genes but not genes regulated solely by H-NS.
RLRR 14:17 (= RLRR 14:17) mutation to ALRA: Still interacts with H-NS, derepresses expression of H-NS/Hha coregulated genes but not genes regulated solely by H-NS.
R17 (= R17) mutation to A: Still interacts with H-NS, derepresses expression of H-NS/Hha coregulated genes but not genes regulated solely by H-NS.
R26 (= R26) mutation to A: Still interacts with H-NS, derepresses expression of H-NS/Hha coregulated genes but not genes regulated solely by H-NS.
D48 (= D48) mutation to A: No longer interacts with H-NS, derepresses expression of H-NS/Hha coregulated genes but not genes regulated solely by H-NS.

P0A3X1 Modulating protein YmoA; Histone-like protein from Yersinia enterocolitica (see paper)
82% identity, 93% coverage: 6:72/72 of query aligns to 1:67/67 of P0A3X1

query
sites
P0A3X1

L

M

T

T

K

K

T

T

D

D

Y

Y

L

L

M

M

R

R

L

L

R

R

R

K

C

C

Q

T

T

T

I

I

D

D

T

T

L

L

E

E

R

R

V

V

I

I

E

E

K

K

N

N

K

K

Y

Y

E

E

L

L

S

S

D

D

N

D

E

E

L

L

A

E

V

L

F

F

Y

Y

S

S

A

A

D
|
D

H

H

R

R

L

L

A

A

E

E

L

L

T

T

M

M

N

N

K

K

L

L

Y

Y

D

D

K

K

I

I

P

P

S

P

S

T

V

V

W

W

K

Q

F

H

I

V

R

K

D43 (= D48) mutation to N: Alters structural dynamics of free protein, loss of H-NS binding (tested with E.coli H-NS fragment 1-64).

P64467 OriC-binding nucleoid-associated protein; H-NS/StpA-binding protein 2; Transcription modulator YdgT from Escherichia coli (strain K12) (see paper)
38% identity, 93% coverage: 6:72/72 of query aligns to 1:71/71 of P64467

query
sites
P64467

L

M

T

T

K

V

T

Q

D

D

Y

Y

L

L

M

L

R

K

L

F

R

R

R

K

C

I

Q

S

T

S

I

L

D

E

T

S

L

L

E

E

R

K

V

L

I

Y

E

D

K

H

N

L

K

N

Y

Y

E

T

L

L

S

T

D

D

N

D

-

Q

E

E

L

L

A

I

V

N

F

M

Y

Y

S

R

A

A

D
|
D

H

H

R

R

L

R

A

A

E

E

L

L

-

V

T

S

M

G

N

G

K

R

L

L

Y

F

D

D

-

L

-

G

K

Q

I

V

P

P

S

K

S

S

V

V

W

W

K

H

F

Y

I

V

R

Q

D44 (= D48) mutation to N: Abolishes heterocomplex formation with H-NS.

Query Sequence

>OHPLBJKB_03214
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLY
DKIPSSVWKFIR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory