SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PFLU_RS30215 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

8wk0N Helicase HerA central domain-containing protein
50% identity, 99% coverage: 2:69/69 of query aligns to 475:542/542 of 8wk0N

query
sites
8wk0N

N

N

R

I

D

K

A

R

V

L

S

P

S

V

S

S

N

N

I

I

I

L

S

S

V

I

G

G

Y

Y

D

E

S

A

G

D

S

S

E

M

T

T

L

L

E

E

I

I

E

E

F

F

K

N

D

H

G

G

A

L

V

V

Y

Y

Q

Q

Y

Y

Y

Y

N

D

V

V

S

P

E

E

H

T

L

L

Y

H

E

T

Q

E

F

L

K

L

A

A

S

A

S

E

S

S

K

H

G

G

Q

K

F

F

F

F

H

N

I

S

Y

Q

I

I

K

K

N

N

A

N

V

Y

P

R

F

F

S

S

R

R

V

I

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 114, 117, 118, 119, 120, 428

8wivD Helicase HerA central domain-containing protein
54% identity, 88% coverage: 9:69/69 of query aligns to 542:602/602 of 8wivD

query
sites
8wivD

S

S

N

N

I

I

I

L

S

S

V

I

G

G

Y

Y

D

E

S

A

G

D

S

S

E

M

T

T

L

L

E

E

I

I

E

E

F

F

K

N

D

H

G

G

A

L

V

V

Y

Y

Q

Q

Y

Y

Y

Y

N

D

V

V

S

P

E

E

H

T

L

L

Y

H

E

T

Q

E

F

L

K

L

A

A

S

A

S

E

S

S

K

H

G

G

Q

K

F

F

F

F

H

N

I

S

Y

Q

I

I

K

K

N

N

A

N

V

Y

P

R

F

F

S

S

R

R

V

I

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 155, 158, 159, 160, 161, 394, 395, 481
binding magnesium ion: 160, 190, 394

8wivO Helicase HerA central domain-containing protein
50% identity, 99% coverage: 2:69/69 of query aligns to 590:657/657 of 8wivO

query
sites
8wivO

N

N

R

I

D

K

A

R

V

L

S

P

S

V

S

S

N

N

I

I

I

L

S

S

V

I

G

G

Y

Y

D

E

S

A

G

D

S

S

E

M

T

T

L

L

E

E

I

I

E

E

F

F

K

N

D

H

G

G

A

L

V

V

Y

Y

Q

Q

Y

Y

Y

Y

N

D

V

V

S

P

E

E

H

T

L

L

Y

H

E

T

Q

E

F

L

K

L

A

A

S

A

S

E

S

S

K

H

G

G

Q

K

F

F

F

F

H

N

I

S

Y

Q

I

I

K

K

N

N

A

N

V

Y

P

R

F

F

S

S

R

R

V

I

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 155, 158, 159, 160, 161, 189, 436
binding magnesium ion: 160, 189, 436

Query Sequence

>PFLU_RS30215
MNRDAVSSSNIISVGYDSGSETLEIEFKDGAVYQYYNVSEHLYEQFKASSSKGQFFHIYI
KNAVPFSRV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory