SitesBLAST
Comparing PP_0159 FitnessBrowser__Putida:PP_0159 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3ubmB Formyl-coa:oxalate coa-transferase from acetobacter aceti (see paper)
32% identity, 99% coverage: 4:405/406 of query aligns to 5:427/430 of 3ubmB
- active site: Q17 (≠ L16), E140 (≠ D146), D182 (= D179), G261 (vs. gap), G262 (vs. gap)
- binding coenzyme a: V16 (= V15), R38 (= R37), L72 (≠ I78), N73 (≠ D79), T74 (≠ F80), K75 (≠ T81), N96 (= N102), F97 (= F103), R98 (≠ K104), A101 (≠ G107), R104 (≠ A110), K125 (≠ T131), D182 (= D179), M213 (≠ L210)
1pt5A Crystal structure of gene yfdw of e. Coli (see paper)
31% identity, 98% coverage: 4:401/406 of query aligns to 4:410/415 of 1pt5A
- active site: Q16 (≠ L16), E139 (≠ D146), D168 (= D179), G247 (vs. gap), G248 (vs. gap)
- binding acetyl coenzyme *a: V15 (= V15), S17 (≠ A17), R37 (= R37), L71 (≠ I78), N72 (≠ D79), T73 (≠ F80), K74 (≠ T81), N95 (= N102), F96 (= F103), H97 (≠ K104), K124 (≠ T131), K136 (≠ A143), A137 (≠ G144), Y138 (= Y145), E139 (≠ D146), D168 (= D179), M199 (≠ L210)
P69902 Formyl-CoA:oxalate CoA-transferase; FCOCT; Formyl-coenzyme A transferase; Formyl-CoA transferase; EC 2.8.3.16 from Escherichia coli (strain K12) (see paper)
31% identity, 98% coverage: 4:401/406 of query aligns to 5:411/416 of P69902
1q6yA Hypothetical protein yfdw from e. Coli bound to coenzyme a (see paper)
31% identity, 98% coverage: 4:401/406 of query aligns to 5:411/417 of 1q6yA
- active site: Q17 (≠ L16), E140 (≠ D146), D169 (= D179), G248 (vs. gap), G249 (vs. gap)
- binding coenzyme a: V16 (= V15), Q17 (≠ L16), S18 (≠ A17), R38 (= R37), L72 (≠ I78), N73 (≠ D79), T74 (≠ F80), K75 (≠ T81), N96 (= N102), F97 (= F103), H98 (≠ K104), M105 (≠ Y111), I124 (= I130), K137 (≠ A143), A138 (≠ G144), Y139 (= Y145), D169 (= D179), M200 (≠ L210)
5yx6A Crystal structure of rv3272 from m. Tuberculosis orthorhombic form (see paper)
33% identity, 94% coverage: 4:386/406 of query aligns to 6:360/360 of 5yx6A
1q7eA Crystal structure of yfdw protein from e. Coli (see paper)
31% identity, 98% coverage: 4:401/406 of query aligns to 5:404/410 of 1q7eA
- active site: Q17 (≠ L16), E133 (≠ D146), D162 (= D179), G241 (vs. gap), G242 (vs. gap)
- binding methionine: N96 (= N102), F97 (= F103), H98 (≠ Y111), P99 (≠ G112), K118 (≠ T131), K130 (≠ A143), A131 (≠ G144), W246 (vs. gap), F299 (≠ A294), A303 (≠ P298), E306 (≠ R301)
O06644 Formyl-CoA:oxalate CoA-transferase; FCOCT; Formyl-coenzyme A transferase; EC 2.8.3.16 from Oxalobacter formigenes (see 4 papers)
28% identity, 99% coverage: 4:406/406 of query aligns to 5:428/428 of O06644
- Q17 (≠ L16) mutation to A: 45-fold decrease of the catalytic effiency.
- R38 (= R37) binding
- W48 (= W47) mutation to F: Little change in the affinity binding and catalytic efficiency, and it does not display major structural changes.; mutation to P: Little change in the affinity binding and catalytic efficiency. It exhibits substrate inhibition with oxalate. It does not display major structural changes.
- R104 (≠ A110) binding
- D169 (= D179) active site, Nucleophile; mutation to A: Loss of CoA-transferase activity.; mutation to E: Loss of CoA-transferase activity.; mutation to S: Loss of CoA-transferase activity.
- G259 (≠ H239) mutation to A: 2.5-fold decrease of the catalytic effiency.
- G260 (≠ P240) mutation to A: 25-fold decrease of the catalytic effiency. Reduction of the affinity binding for both formyl-CoA and oxalate.
1p5rA Formyl-coa transferase in complex with coenzyme a (see paper)
28% identity, 99% coverage: 4:406/406 of query aligns to 4:427/427 of 1p5rA
- active site: Q16 (≠ L16), E139 (≠ D146), D168 (= D179), G259 (≠ P240), G260 (≠ N241)
- binding coenzyme a: H14 (≠ R14), V15 (= V15), Q16 (≠ L16), A17 (= A17), R37 (= R37), M73 (≠ F80), K74 (≠ T81), N95 (= N102), F96 (= F103), A100 (≠ G107), R103 (≠ A110), K136 (≠ A143), V137 (≠ G144), D168 (= D179), M199 (≠ L210)
2vjoA Formyl-coa transferase mutant variant q17a with aspartyl-coa thioester intermediates and oxalate (see paper)
28% identity, 99% coverage: 4:406/406 of query aligns to 4:427/427 of 2vjoA
- active site: A16 (≠ L16), E139 (≠ D146), D168 (= D179), G259 (≠ P240), G260 (≠ N241)
- binding coenzyme a: H14 (≠ R14), A16 (≠ L16), A17 (= A17), R37 (= R37), L71 (≠ I78), M73 (≠ F80), N95 (= N102), F96 (= F103), G97 (≠ K104), R103 (≠ A110), M104 (≠ Y111), K136 (≠ A143), V137 (≠ G144), Y138 (= Y145), D168 (= D179), M199 (≠ L210)
- binding oxalate ion: G257 (≠ A238), G259 (≠ P240), Q261 (vs. gap)
2vjkA Formyl-coa transferase with aspartyl-coa thioester intermediate derived from oxalyl-coa (see paper)
28% identity, 99% coverage: 4:406/406 of query aligns to 4:427/427 of 2vjkA
- active site: Q16 (≠ L16), E139 (≠ D146), D168 (= D179), G259 (≠ P240), G260 (≠ N241)
- binding coenzyme a: H14 (≠ R14), Q16 (≠ L16), A17 (= A17), R37 (= R37), M73 (≠ F80), K74 (≠ T81), N95 (= N102), F96 (= F103), G97 (≠ K104), R103 (≠ A110), M104 (≠ Y111), K136 (≠ A143), V137 (≠ G144), Y138 (= Y145), D168 (= D179), M199 (≠ L210)
- binding magnesium ion: D293 (≠ E270), D296 (≠ G273)
1t4cA Formyl-coa transferase in complex with oxalyl-coa (see paper)
28% identity, 99% coverage: 4:406/406 of query aligns to 4:427/427 of 1t4cA