SitesBLAST

244 G→F: Disrupts ergothioneine (EGT) binding. Binds EGT 100-fold more weakly. May have a global impact on overall structure.
293 F→Y: Disrupts ergothioneine (EGT) binding.
375 E→Q: Disrupts ergothioneine (EGT) binding. Binds EGT 500-fold more weakly.
419 Y→F: Disrupts ergothioneine (EGT) binding.

B5Z7I3 Ergothioneine transport permease/ergothioneine binding protein EgtU from Helicobacter pylori (strain G27) (see paper)
32% identity, 38% coverage: 105:211/279 of query aligns to 78:184/553 of B5Z7I3

query
sites
B5Z7I3

V

I

G

G

I

V

A

F

I

A

G

F

Y

Y

N

N

R

S

V

K

A

A

R

R

G

A

I

F

L

L

D

L

P

P

L

V

I

V

E

N

F

F

Y

L

R

Y

P

T

I

I

P

P

P

S

L

L

A

A

Y

L

L

F

P

A

L

L

I

F

V

I

I

P

W

V

C

I

G

G

I

V

G

G

E

L

L

K

S

N

K

A

V

L

L

L

L

V

I

L

Y

V

L

L

A

Y

I

G

F

L

A

L

P

P

I

I

A

V

I

Y

A

S

T

T

A

Y

T

N

G

A

V

L

R

K

T

E

V

V

D

R

P

E

A

E

K

V

L

I

R

K

A

A

Q

I

S

G

L

L

G

G

A

C

T

N

K

P

A

K

Q

E

L

L

I

F

R

F

H

R

V

V

I

H

L

F

P

L

S

L

A

A

L

I

P

P

D

Q

I

I

L

L

T

A

G

G

I

L

R

R

I

I

G

A

L

V

Sites not aligning to the query:

390 Y→A: Disrupts EGT binding.; Y→F: Retains EGT binding.
454 R→A: Retains EGT binding.; R→E: Disrupts EGT binding.

Query Sequence

>PP_0231 FitnessBrowser__Putida:PP_0231
MSSLDLPVAGKHNTQIRPAATLRRPLSTRWISTLTLASLLMVWWLVTAAGWVEPLFLPSP
GDILAKAWTLLTQGYMDASLWQHLSASLGRIGLALVAATLTAIPVGIAIGYNRVARGILD
PLIEFYRPIPPLAYLPLIVIWCGIGELSKVLLIYLAIFAPIAIATATGVRTVDPAKLRAA
QSLGATKAQLIRHVILPSALPDILTGIRIGLGVGWSTLVAAELIAATSGLGFMVQSAAQF
LVTDVVVLGILLIALIAFALEMSLRALQRKLVPWHGQIH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory