SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PP_0328 FitnessBrowser__Putida:PP_0328 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P46154 Glutathione-independent formaldehyde dehydrogenase; FALDH; FDH; Formaldehyde dismutase; EC 1.2.1.46; EC 1.2.98.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see paper)
94% identity, 100% coverage: 1:399/399 of query aligns to 1:399/399 of P46154

query
sites
P46154

M

M

S

S

G

G

N

N

R

R

G

G

V

V

Y

Y

L

L

G

G

A

S

G

G

K

K

V

V

E

E

V

V

Q

Q

K

K

I

I

D

D

Y

Y

P

P

K

K

M

M

Q

Q

D

D

P

P

R

R

G

G

K

K

I

I

E

E

H

H

G

G

V

V

I

I

L

L

K

K

V

V

S

S

T

T

N

N

I

I

C
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C

G
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G

S
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S

D
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D

Q
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Q

H
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H

M

M

V

V

R

R

G

G

R

R

T

T

A

A

Q

Q

V

V

G

G

L

L

V

V

L

L

G

G

H
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H

E

E

I

I

T

T

G

G

E

E

I

V

V

I

E

E

K

K

G

G

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R

D

D

V

V

E

E

R

N

M

L

Q

Q

I

I

G

G

D

D

L

L

V

V

S

S

V

V

P

P

F

F

N

N

V

V

A

A

C
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C

G

G

R

R

C
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C

R

R

S

S

C
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C

K

K

E

E

M

M

H

H

T

T

G

G

V

V

C
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C

L

L

T

T

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V

N

N

P

P

A

A

R

R

A

A

G

G

A

A

Y

Y

G

G

Y

Y

V

V

D

D

M

M

G

G

D

D

W

W

T

T

G

G

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Q

A

A

E

E

Y

Y

V

L

L

L

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V

P

P

Y

Y

A

A

D

D

F

F

N

N

L

L

K

K

L

L

P

P

E

D

R

R

D

D

K

K

A

A

M

M

E

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K

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I

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D

L

L

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T

C

C

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L

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S

D

D

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I

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P

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T

G

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Y

Y

H

H

G

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A

A

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T

T

A

A

G

G

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G

G

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P

G

G

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S

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T

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Y

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V

A

A

G

G

A

A

G

G

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V
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V

G

G

L

L

A

A

S

S

A

A

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R

L

L

G

G

A

A

C

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V

V

I

I

V

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G

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D
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D

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L

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A

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R

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L

A

A

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H

A

A

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K

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Q

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G

F

F

E

E

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A

D

D

L

L

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D

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L

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E

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L

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G

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P

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D

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C

A

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A

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V

G

G

F

F

E

E

A

A

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R

G
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G

H
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H

G

G

H

H

E

E

G

G

A

A

K

K

H

H

E

E

A

A

P

P

A

A

T

T

V

V

L

L

N

N

S

S

L

L

M

M

Q

Q

V

V

T

T

R

R

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V

A

A

G

G

N

K

I

I

G

G

I

I

P
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P

G
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L
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L

Y

Y

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T

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E

D

D

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P

G

G

A

A

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D

D

A

A

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K

I

I

G

G

A

S

L

L

S

S

I

I

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R

F

F

G

G

L

L

G

G

W

W

A

A

K

K

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H

H

S

S

F

F

H

H

T

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G
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G

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T

V

M

M

K

K

Y

Y

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N

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L

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M

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Q

A

A

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I

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M

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W

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D

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R

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I

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N

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I

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E

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A

C47 (= C47) binding
GSDQH 48:52 (= GSDQH 48:52) binding
H68 (= H68) binding
C98 (= C98) binding
C101 (= C101) binding
C104 (= C104) binding
C112 (= C112) binding
V198 (= V198) binding
D218 (= D218) binding
R223 (= R223) binding
V263 (= V263) binding
RGH 268:270 (= RGH 268:270) binding
PGL 300:302 (= PGL 300:302) binding
GQT 337:339 (= GQT 337:339) binding

1kolA Crystal structure of formaldehyde dehydrogenase (see paper)
94% identity, 99% coverage: 3:398/399 of query aligns to 1:396/396 of 1kolA

query
sites
1kolA

G

G

N

N

R

R

G

G

V

V

Y

Y

L

L

G

G

A

S

G

G

K

K

V

V

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E

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V

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Q

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K

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I

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D

Y

Y

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P

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K

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M

Q

Q

D

D

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P

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G

G

K

K

I

I

E

E

H

H

G

G

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V

I

I

L

L

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K

V

V

S

S

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T

N

N

I

I

C
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C

G
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G

S
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S

D

D

Q

Q

H
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H

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M

V

V

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R

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G

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A

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Q

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G

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L

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V

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L

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E
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E

I

I

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T

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E

E

I

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V

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E

E

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K

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G

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D

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Q

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I

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G

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D

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L

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S

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F
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F

N

N

V

V

A

A

C
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C

G

G

R

R

C
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C

R

R

S

S

C
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C

K

K

E

E

M

M

H

H

T

T

G

G

V

V

C
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C

L

L

T

T

V

V

N
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N

P

P

A

A

R

R

A

A

G

G

A

A

Y

Y

G

G

Y

Y

V

V

D

D

M

M

G

G

D

D

W

W

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T

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G

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Q

A

A

E

E

Y

Y

V

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L

L

V

V

P

P

Y

Y

A

A

D

D

F

F

N

N

L

L

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K

L

L

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P

E

D

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R

D

D

K

K

A

A

M

M

E

E

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K

I

I

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R

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D

L

L

T

T

C

C

L

L

S

S

D
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D

I

I

L

L

P

P

T
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T

G

G

Y

Y

H

H

G

G

A

A

V

V

T

T

A

A

G

G

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V

G

G

P

P

G

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T

T

V

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Y

Y

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V

A

A

G
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G

A

A

G
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G

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P

V
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V

G

G

L

L

A

A

S

S

A

A

R

R

L

L

G

G

A

A

C

V

V

V

I

I

V

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G

G

D
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D

L
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L

N

N

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P

A

A

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R

L

L

A

A

H

H

A

A

K

K

S

A

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Q

G

G

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F

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E

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I

V

A

D

D

L

L

S

S

K

L

D

D

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P

L

L

H

H

E

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I

I

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A

D

A

I

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L

G

G

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E

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P

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E

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V

D

D

C

C

A

A

V

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D

D

A
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A

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V

G

G

F

F

E

E

A

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R
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R

G

G

H
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H

G

G

H

H

E

E

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G

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A

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K

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H

E

E

A

A

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P

A

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T

V
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V

L

L

N

N

S

S

L

L

M

M

Q

Q

V

V

T

T

R

R

V

V

A

A

G

G

N

K

I

I

G

G

I

I

P
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P

G

G

L
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L

Y

Y

V

V

T

T

E

E

D

D

P

P

G

G

A

A

V

V

D

D

A

A

K

K

I

I

G

G

A

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L

L

S

S

I

I

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R

F

F

G

G

L

L

G

G

W

W

A

A

K

K

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S

H

H

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S

F

F

H

H

T

T

G

G

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Q

T
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T

P

P

T

V

M

M

K

K

Y

Y

N

N

R

R

Q

A

L

L

M

M

Q

Q

A

A

I

I

M

M

W

W

D

D

R

R

I

I

N

N

I

I

A

A

E

E

V

V

G

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Q

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V

I

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N

S

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L

D

D

Q

D

A

A

P

P

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R

G

G

Y

Y

G

G

E

E

F

F

D

D

A

A

G

G

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K

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F

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A

A

active site: C45 (= C47), G46 (= G48), S47 (= S49), H50 (= H52), H66 (= H68), E67 (= E69), C96 (= C98), C99 (= C101), C102 (= C104), C110 (= C112), N114 (= N116), D168 (= D170), T172 (= T174), K385 (= K387)
binding nicotinamide-adenine-dinucleotide: C45 (= C47), G46 (= G48), S47 (= S49), H50 (= H52), F92 (= F94), D168 (= D170), T172 (= T174), G192 (= G194), G194 (= G196), P195 (= P197), V196 (= V198), D216 (= D218), L217 (= L219), R221 (= R223), A260 (= A262), V261 (= V263), R266 (= R268), H268 (= H270), V281 (= V283), P298 (= P300), L300 (= L302), Q336 (= Q338), T337 (= T339)
binding zinc ion: C45 (= C47), H66 (= H68), C96 (= C98), C99 (= C101), C102 (= C104), C110 (= C112), D168 (= D170)

4jlwA Crystal structure of formaldehyde dehydrogenase from pseudomonas aeruginosa (see paper)
87% identity, 99% coverage: 3:396/399 of query aligns to 1:394/395 of 4jlwA

query
sites
4jlwA

G

G

N

N

R

R

G

G

V

V

Y

Y

L

L

G

G

A

P

G

G

K

K

V

V

E

E

V

V

Q

Q

K

N

I

I

D

P

Y

Y

P

P

K

K

M

M

Q

Q

D

D

P

P

R

Q

G

G

K

R

K

Q

I

I

E

D

H

H

G

G

V

V

I

I

L

L

K

R

V

V

S

S

T

T

N

N

I

I

C
|
C

G
|
G

S
|
S

D

D

Q

Q

H
|
H

M

M

V

V

R

R

G

G

R

R

T

T

A

A

Q

P

V

E

G

G

L

L

V

V

L

L

G

G

H
|
H

E

E

I

I

T

T

G

G

E

E

I

V

V

V

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E

K

I

G

G

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R

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G

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V

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E

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M

M

Q

K

I

I

G

G

D

D

L

L

V

V

S

S

V

V

P

P

F

F

N

N

V

V

A

A

C
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C

G

G

R

H

C
|
C

R

R

S

T

C
|
C

K

K

E

E

M

Q

H

H

T

T

G

G

V

V

C
|
C

L

L

T

T

V

V

N

N

P

P

A

A

R

R

A

A

G

G

A

A

Y

Y

G

G

Y

Y

V

V

D

D

M

M

G

G

D

G

W

W

T

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G

G

Q

Q

A

A

E

E

Y

Y

V

V

L

L

V

V

P

P

Y

Y

A

A

D

D

F

F

N

N

L

L

K

K

L

L

P

P

E

N

R

R

D

E

K

A

A

A

M

M

E

E

K

K

I

I

R

R

D

D

L

L

T

T

C

C

L

L

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S

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D

I

I

L

L

P

P

T
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T

G

G

Y

Y

H

H

G

G

A

A

V

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T

T

A

A

G

G

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V

G

G

P

P

G

G

S

S

T

T

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V

Y

Y

V

I

A

A

G

G

A

A

G
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G

P
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P

V
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V

G

G

L

L

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A

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S

A

A

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R

L

L

G

G

A

A

C

V

V

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I

I

V

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G

G

D
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D

L

V

N

N

P

P

A

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R
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R

L

L

A

A

H

H

A

A

K

K

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K

Q

Q

G

G

F

F

E

E

V

I

V

A

D

D

L

L

S

S

K

K

D

D

T

T

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P

L

L

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E

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Q

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A

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A

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L

G

G

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P

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E

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D

C

C

A

A

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D

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A

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V

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G

F

F

E

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A

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R

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H

G

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H

H

E

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G

G

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S

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Q

H

Q

E

E

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A

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P

A

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V

L

L

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N

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S

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L

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M

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T

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R

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V

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A

G

G

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K

I

I

G

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P

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L

Y

Y

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T

E

E

D

D

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P

G

G

A

A

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V

D

D

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A

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K

I

H

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G

A

A

L

L

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S

I

I

R

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F

F

G

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L

L

G

G

W

W

A

A

K

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H

H

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S

F

F

H

H

T

T

G

G

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Q

T
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T

P

P

T

V

M

M

K

K

Y

Y

N

N

R

R

Q

Q

L

L

M

M

Q

Q

A

A

I

I

M

M

W

W

D

D

R

R

I

I

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K

I

I

A

A

E

D

V

I

V

V

G

G

V

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Q

E

V

V

I

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N

T

L

L

D

D

Q

D

A

A

P

P

E

K

G

G

Y

Y

G

G

E

E

F

F

D

D

A

A

G

G

V

V

P

P

K

K

F

F

V

V

I

I

D

D

P

P

H

H

K

N

M

L

W

F

binding nicotinamide-adenine-dinucleotide: C45 (= C47), G46 (= G48), S47 (= S49), H50 (= H52), D168 (= D170), T172 (= T174), G194 (= G196), P195 (= P197), V196 (= V198), D216 (= D218), R221 (= R223), V261 (= V263), R266 (= R268), H268 (= H270), V281 (= V283), P298 (= P300), L300 (= L302), Q336 (= Q338), T337 (= T339)
binding zinc ion: C45 (= C47), H66 (= H68), C96 (= C98), C99 (= C101), C102 (= C104), C110 (= C112), D168 (= D170)

Q52078 Formaldehyde dismutase; EC 1.2.98.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see paper)
60% identity, 99% coverage: 1:395/399 of query aligns to 1:395/399 of Q52078

query
sites
Q52078

M
|
M

S
x
A

G
|
G

N
|
N

R
x
K

G
x
S

V
|
V

Y
|
Y

L
x
H

G
|
G

A
x
T

G
x
R

K
x
D

V
x
L

E
x
R

V
|
V

Q
x
E

K
x
T

I
x
V

D
x
P

Y
|
Y

P
|
P

K
|
K

M
x
L

Q
x
E

D
x
H

P

-

R
x
N

G
x
N

K
x
R

K
|
K

I
x
L

E
|
E

H
|
H

G
x
A

V
|
V

I
|
I

L
|
L

K
|
K

V
|
V

S
|
S

T
|
T

N
|
N

I
|
I

C
|
C

G
|
G

S
|
S

D
|
D

Q
|
Q

H
|
H

M
x
I

V
x
Y

R
|
R

G
|
G

R
|
R

T
x
F

T
x
I

A
x
V

Q
x
P

V
x
K

G
|
G

L
x
H

V
|
V

L
|
L

G
|
G

H
|
H

E
|
E

I
|
I

T
|
T

G
|
G

E
|
E

I
x
V

V
|
V

E
|
E

K
|
K

G
|
G

R
x
S

D
|
D

V
|
V

E
|
E

R
x
L

M
|
M

Q
x
D

I
|
I

G
|
G

D
|
D

L
|
L

V
|
V

S
|
S

V
|
V

P
|
P

F
|
F

N
|
N

V
|
V

A
|
A

C
|
C

G
|
G

R
|
R

C
|
C

R
|
R

S
x
N

C
|
C

K
|
K

E
|
E

M
x
A

H
x
R

T
x
S

G
x
D

V
|
V

C
|
C

L
x
E

T
x
N

-
x
N

-
x
L

V
|
V

N
|
N

P
|
P

A
x
D

R
x
A

A
x
D

G
x
L

G
|
G

A
|
A

Y
x
F

G
|
G

Y
x
F

V

-

D
|
D

M
x
L

G
x
K

D
x
G

W
|
W

T
x
S

G
|
G

Q
|
Q

A
|
A

E
|
E

Y
|
Y

V
|
V

L
|
L

V
|
V

P
|
P

Y
|
Y

A
|
A

D
|
D

F
x
Y

N
x
M

L
|
L

K
|
K

L
x
F

P
x
G

E
x
D

R
x
K

D
x
E

K
x
Q

A
|
A

M
|
M

E
|
E

K
|
K

I
|
I

R
x
K

D
|
D

L
|
L

T
|
T

C
x
L

L
x
I

S
|
S

D
|
D

I
|
I

L
|
L

P
|
P

T
|
T

G
|
G

Y
x
F

H
|
H

G
|
G

A
x
C

V
|
V

T
x
S

A
|
A

G
|
G

V
|
V

G
x
K

P
|
P

G
|
G

S
|
S

T
x
H

V
|
V

Y
|
Y

V
x
I

A
|
A

G
|
G

A
|
A

G
|
G

P
|
P

V
|
V

G
|
G

L
x
R

A
x
C

A
|
A

S
x
G

A
|
A

R
|
R

L
|
L

G
|
G

A
|
A

C
|
C

V
|
V

I
|
I

V
|
V

G
|
G

D
|
D

L
x
Q

N
|
N

P
|
P

A
x
E

R
|
R

L
|
L

A
x
K

H
x
L

A
x
L

K
x
S

S
x
D

Q
x
A

G
|
G

F
|
F

E
|
E

V
x
T

V
x
I

D
|
D

L
|
L

S
x
R

K
x
N

D
x
S

T
x
A

P
|
P

L
|
L

H
x
R

E
x
D

Q
|
Q

I
|
I

V
x
D

D
x
Q

I
|
I

L
|
L

G
|
G

E
x
K

P
|
P

E
|
E

V
|
V

D
|
D

C
|
C

A
x
G

V
|
V

D
|
D

A
|
A

V
|
V

G
|
G

F
|
F

E
|
E

A
|
A

R
x
H

G
|
G

H
x
L

G
|
G

H
x
D

E
|
E

G

-

A
|
A

K
x
N

H
x
T

E
|
E

A
x
T

P
|
P

A
x
N

T
x
G

V
x
A

L
|
L

N
|
N

S
|
S

L
|
L

M
x
F

Q
x
D

V
|
V

T
x
V

R
|
R

V
x
A

A
x
G

G
|
G

N
x
A

I
|
I

G
|
G

I
|
I

P
|
P

G
|
G

L
x
I

Y
|
Y

V
|
V

T
x
G

E
x
S

D
|
D

P
|
P

G
x
D

A
x
P

V
|
V

D
x
N

A
x
K

A
x
D

A
|
A

K
x
G

I
x
S

G
|
G

A
x
R

L
|
L

S
x
H

I
x
L

R
x
D

F
|
F

G
|
G

L
x
K

G
x
M

W
|
W

A
x
T

K
|
K

S
|
S

H
x
I

S
x
R

F
x
I

H
x
M

T
|
T

G
|
G

Q
x
M

T
x
A

P
|
P

T
x
V

M
x
T

K
x
N

Y
|
Y

N
|
N

R
|
R

Q
x
H

L
|
L

M
x
T

Q
x
E

A
|
A

I
|
I

M
x
L

W
|
W

D
|
D

R
x
Q

I
x
M

-
x
P

N
x
Y

I
x
L

A
x
S

E
x
K

V
|
V

V
x
M

G
x
N

V
x
I

Q
x
E

V
|
V

I
|
I

N
x
T

L
|
L

D
|
D

Q
|
Q

A
|
A

P
|
P

E
x
D

G
|
G

Y
|
Y

G
x
A

E
x
K

F
|
F

D
|
D

A
x
K

G
|
G

V
x
S

P
|
P

K
x
A

K
|
K

F
|
F

V
|
V

I
|
I

D
|
D

P
|
P

H
|
H

K
x
G

M
|
M

M1 (= M1) modified: Initiator methionine, Removed
C46 (= C47) binding
GSDQH 47:51 (= GSDQH 48:52) binding
H67 (= H68) binding
C97 (= C98) binding
C100 (= C101) binding
C103 (= C104) binding
C111 (= C112) binding
D170 (= D170) binding
T174 (= T174) binding
PV 197:198 (= PV 197:198) binding
DQ 218:219 (≠ DL 218:219) binding
R223 (= R223) binding
V263 (= V263) binding
H268 (≠ R268) binding
P299 (= P300) binding
PGI 299:301 (≠ PGL 300:302) binding
GMA 336:338 (≠ GQT 337:339) binding

Sites not aligning to the query:

2:399 modified: mature protein, Formaldehyde dismutase

2dphA Crystal structure of formaldehyde dismutase
60% identity, 99% coverage: 2:395/399 of query aligns to 1:394/398 of 2dphA

query
sites
2dphA

S

A

G

G

N

N

R

K

G

S

V

V

Y

Y

L

H

G

G

A

T

G

R

K

D

V

L

E

R

V

V

Q

E

K

T

I

V

D

P

Y

Y

P

P

K

K

M

L

Q

E

D

H

P

-

R

N

G

N

K

R

K

K

I

L

E

E

H

H

G

A

V

V

I

I

L

L

K

K

V

V

S

S

T

T

N

N

I

I

C
|
C

G
|
G

S
|
S

D

D

Q

Q

H
|
H

M

I

V

Y

R

R

G

G

R

R

T

F

T

I

A

V

Q

P

V

K

G

G

L

H

V

V

L

L

G

G

H
|
H

E
|
E

I

I

T

T

G

G

E

E

I

V

V

V

E

E

K

K

G

G

R

S

D

D

V

V

E

E

R

L

M

M

Q

D

I

I

G

G

D

D

L

L

V

V

S

S

V

V

P

P

F
|
F

N

N

V

V

A

A

C
|
C

G

G

R

R

C
|
C

R

R

S

N

C
|
C

K

K

E

E

M

A

H

R

T

S

G

D

V

V

C
|
C

L

E

T

N

-

N

-
x
L

V

V

N

N

P

P

A

D

R

A

A

D

G

L

G

G

A

A

Y

F

G

G

Y

F

V

-

D

D

M

L

G

K

D

G

W

W

T

S

G

G

Q

Q

A

A

E

E

Y

Y

V

V

L

L

V

V

P

P

Y

Y

A

A

D

D

F

Y

N

M

L

L

K

K

L

F

P

G

E

D

R

K

D

E

K

Q

A

A

M

M

E

E

K

K

I

I

R

K

D

D

L

L

T

T

C

L

L

I

S
|
S

D
|
D

I

I

L

L

P
|
P

T
|
T

G

G

Y

F

H

H

G

G

A

C

V

V

T

S

A

A

G

G

V

V

G

K

P

P

G

G

S

S

T

H

V

V

Y

Y

V

I

A
|
A

G
|
G

A

A

G
|
G

P
|
P

V
|
V

G

G

L

R

A

C

A

A

S

G

A

A

R

R

L

L

G

G

A

A

C

C

V

V

I

I

V

V

G
|
G

D
|
D

L
x
Q

N

N

P

P

A

E

R
|
R

L

L

A

K

H

L

A

L

K

S

S

D

Q

A

G

G

F

F

E

E

V

T

V

I

D

D

L
|
L

S

R

K

N

D

S

T

A

P

P

L

L

H

R

E

D

Q

Q

I

I

V

D

D

Q

I

I

L

L

G

G

E

K

P

P

E

E

V

V

D

D

C

C

A

G

V

V

D

D

A

A

V
|
V

G

G

F

F

E

E

A

A

R
x
H

G

G

H

L

G

G

H

D

E

E

G

-

A

A

K

N

H

T

E

E

A

T

P

P

A

N

T

G

V

A

L

L

N

N

S

S

L

L

M

F

Q

D

V

V

T

V

R

R

V

A

A

G

G

G

N

A

I

I

G

G

I

I

P
|
P

G
|
G

L
x
I

Y

Y

V

V

T

G

E

S

D

D

P

P

G

D

A

P

V

V

D

N

A

K

A

D

A

A

K

G

I

S

G

G

A

R

L

L

S

H

I

L

R

D

F

F

G

G

L

K

G

M

W

W

A

T

K

K

S

S

H

I

S

R

F

I

H

M

T

T

G

G

Q

M

T
x
A

P

P

T

V

M

T

K

N

Y

Y

N

N

R

R

Q

H

L

L

M

T

Q

E

A

A

I

I

M

L

W

W

D

D

R

Q

I

M

-

P

N

Y

I

L

A

S

E

K

V

V

V

M

G

N

V

I

Q

E

V

V

I

I

N

T

L

L

D

D

Q

Q

A

A

P

P

E

D

G

G

Y

Y

G

A

E

K

F

F

D

D

A

K

G

G

V

S

P

P

K

A

K
|
K

F

F

V

V

I

I

D

D

P

P

H

H

K

G

M

M

active site: C45 (= C47), G46 (= G48), S47 (= S49), H50 (= H52), H66 (= H68), E67 (= E69), C96 (= C98), C99 (= C101), C102 (= C104), C110 (= C112), L114 (vs. gap), S168 (= S169), D169 (= D170), P172 (= P173), K386 (= K387)
binding nicotinamide-adenine-dinucleotide: C45 (= C47), G46 (= G48), S47 (= S49), H50 (= H52), F92 (= F94), D169 (= D170), T173 (= T174), A192 (= A193), G193 (= G194), G195 (= G196), P196 (= P197), V197 (= V198), G216 (= G217), D217 (= D218), Q218 (≠ L219), R222 (= R223), L236 (= L237), V262 (= V263), H267 (≠ R268), P298 (= P300), G299 (= G301), I300 (≠ L302), A337 (≠ T339)
binding zinc ion: C45 (= C47), H66 (= H68), C96 (= C98), C99 (= C101), C102 (= C104), C110 (= C112), D169 (= D170)

4cpdA Alcohol dehydrogenase tadh from thermus sp. Atn1
30% identity, 94% coverage: 5:381/399 of query aligns to 2:334/346 of 4cpdA

query
sites
4cpdA

R

R

G

A

V

V

Y

F

L

E

G

N

A

K

G

E

K

R

V

V

E

A

V

V

Q

K

K

E

I

V

D

N

Y

A

P

P

K

R

M

L

Q

Q

D

H

P

P

R

L

G

D

K

-

I

-

E

-

H

-

G

-

V

A

I

L

L

V

K

R

V

V

V

H

S

L

T

A

N

G

I

I

C
|
C

G
|
G

S
|
S

D

D

Q

L

H
|
H

M

L

V

Y

R

H

G

G

R

K

T

I

T

P

A

V

Q

L

V

P

G

G

L

S

V

V

L

L

G

G

H
|
H

E
|
E

I

F

T

V

G

G

E

Q

I

V

V

E

E

A

K

V

G

G

R

E

D

G

V

I

E

Q

R

D

M

L

Q

Q

I

P

G

G

D

D

L

W

V

V

S

V

V

G

P

P

F

F

N

H

V

I

A

A

C
|
C

G

G

R

T

C
|
C

R

P

S

Y

C
|
C

K

R

E

R

M

H

H

Q

T

Y

G

N

V

L

C
|
C

L

-

T

-

V

-

N

-

P

-

A

-

R

E

A

R

G

G

G
|
G

A

V

Y

Y

G

G

Y

Y

V

G

D

P

M

M

-

F

G

G

D

N

W

L

T

Q

G

G

G

A

Q

Q

A

A

E

E

Y

I

V

L

L

R

V

V

P

P

Y

F

A

S

D

N

F

V

N

N

L

L

L

R

K

K

L

L

P

P

E

P

R

N

D

L

K

S

A

P

M

E

E

R

K

A

I

I

R

-

D

-

L

-

T

-

C

F

L

A

S

G

D
|
D

I

I

L

L

P

S

T
|
T

G

A

Y

Y

H

G

G

G

A

L

V

I

T

Q

A

G

G

Q

V

L

G

R

P

P

G

G

S

D

T

S

V

V

Y

A

V

V

A

I

G

G

A

A

G
|
G

P
|
P

V
|
V

G

G

L

L

A

M

A

A

A

I

A

E

S

V

A

A

R

Q

L

V

L

L

G

G

A

A

A

S

C

K

V

I

I

L

V

A

G

I

D
|
D

L
x
R

N

I

P

P

A

E

R
|
R

L

L

A

E

H

R

A

A

K

A

S

S

Q

L

G

G

F

A

E

I

V

P

V

I

D

N

L

A

S

E

K

Q

D

E

T

N

P

P

L

V

H

R

E

-

Q

R

I

V

V

R

D

S

I

E

L

T

G

N

E

D

P

E

E

G

V

P

D

D

C

L

A

V

V

L

D

E

A
|
A

V
|
V

G

G

F

G

E

-

A

-

R

-

G

-

H

-

G

-

H

-

E

-

G

-

A

-

K

-

H

-

E

-

A

-

P

-

A

A

T

A

V

T

L

L

N

S

S

L

L

A

M

L

Q

E

V

M

T

V

R

R

V

-

A

-

G

-

N

-

I

-

G

-

I

-

P

P

G

G

L

-

Y

-

V

-

T

-

E

-

D

-

P

-

G

G

A

R

V

V

D

S

A

A

A
x
V

A

G

K
x
V

I

D

G

N

A

A

L

P

S

S

I

F

R

P

F

F

G

P

L

L

-

A

-

S

G

G

W

L

A

V

K

K

S

D

H

L

S

T

F

F

H

R

T

I

G

G

Q
x
L

T
x
A

P

N

T

V

M

H

K

L

Y

Y

N

I

R

D

Q

A

L

V

M

L

Q

A

A

L

I

L

M

A

W

S

D

G

R

R

I

L

N

Q

I

P

A

E

E

R

V

I

V

V

G

S

V

-

Q

H

V

Y

I

L

N

P

L

L

D

E

Q

E

A

A

P

P

E

R

G

G

Y

Y

G

E

E

L

F
|
F

D

D

active site: C38 (= C47), G39 (= G48), S40 (= S49), H43 (= H52), H59 (= H68), E60 (= E69), C89 (= C98), C92 (= C101), C95 (= C104), C103 (= C112), G107 (= G122), D152 (= D170), T156 (= T174)
binding nicotinamide-adenine-dinucleotide: G39 (= G48), S40 (= S49), T156 (= T174), G178 (= G196), P179 (= P197), V180 (= V198), D200 (= D218), R201 (≠ L219), R205 (= R223), A243 (= A262), V244 (= V263), V266 (≠ A314), V268 (≠ K316), L292 (≠ Q338), A293 (≠ T339), F333 (= F380)
binding zinc ion: C38 (= C47), H59 (= H68), C89 (= C98), C92 (= C101), C95 (= C104), C103 (= C112), D152 (= D170)

Sites not aligning to the query:

active site: 340

5ylnA Zinc dependent alcohol dehydrogenase 2 from streptococcus pneumonia - apo form
34% identity, 67% coverage: 1:267/399 of query aligns to 2:246/348 of 5ylnA

query
sites
5ylnA

M

M

S

G

G

S

N

M

R

K

G

A

V

Y

V

T

Y

Y

L

V

G

K

A

P

G

G

K

L

V

A

E

S

V

F

Q

V

K

D

I

V

D

D

Y

K

P

P

K

V

M

I

Q

R

D

K

P

P

R

T

G

A

K

-

I

-

E

-

H

-

G

-

V

-

I

I

L

V

K

R

V

I

V

V

S

K

T

T

N

T

I

I

C
|
C

G

G

S
x
T

D

D

Q

L

H
|
H

M

I

V

I

R

K

G

G

R

D

T

V

-

P

T

T

A

C

Q

Q

V

S

G

G

L

T

V

I

L

L

G

G

H
|
H

E

E

I

G

T

I

G

G

E

I

I

V

V

E

E

E

K

V

G

G

R

E

D

G

V

V

E

S

R

N

M

F

Q

K

I

K

G

G

D

D

L

K

V

V

S

L

V

I

P

S

F

C

N

V

V

C

A

A

C
|
C

G

G

R

K

C
|
C

R

Y

S

Y

C
|
C

K

K

E

K

M

-

H

-

T

-

G

-

V

-

C

-

L

-

T

-

V

-

N

-

P

-

A

-

R

-

A

-

G

-

G

G

A

I

Y

Y

G

A

Y

H

V
x
C

-

E

D

D

M

E

G

G

D

G

W

W

T

I

-

F

-

I

-

D

G

G

G

M

Q

Q

A

A

E

E

Y

Y

V

L

L

R

V

V

P

P

Y

H

A

A

D

D

F

N

N

T

L

L

L

Y

K

H

L

T

P

P

E

E

-

D

-

L

R

S

D

D

K

E

A

A

M

-

E

-

K

-

I

-

R

-

D

-

L

-

T

-

C

-

L

L

S

V

D

M

I

L

L

L

P

P

T

T

G

G

Y

Y

H

E

-

I

G

G

A

V

V

L

T

K

A

G

G

K

V

V

G

E

P

P

G

G

S

C

T

S

V

V

Y

A

V

I

A

I

G

G

A

S

G

G

P

P

V

V

G

G

L

L

A

A

A

L

S

T

A

A

R

Q

L

F

L

Y

G

S

A

P

A

A

C

K

V

L

I

I

V

M

G

V

D

D

L

L

N

D

P

D

A

N

R

R

L

L

A

E

H

T

A

A

K

L

S

S

Q

F

G

G

-

A

F

T

E

H

V

K

V

V

D

N

L

S

S

S

K

D

D

P

T

E

P

K

L

A

H

I

E

K

Q

E

I

I

V

Y

D

D

I

L

L

T

G

D

E

G

P

R

E

G

V

V

D

D

C

V

A

A

V

I

D

E

A

A

V

V

G

G

F

I

E

P

A

A

active site: C41 (= C47), T43 (≠ S49), H46 (= H52), H63 (= H68)
binding zinc ion: C41 (= C47), H63 (= H68), C93 (= C98), C96 (= C101), C99 (= C104), C107 (≠ V127)

P07913 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Escherichia coli (strain K12) (see paper)
31% identity, 66% coverage: 37:300/399 of query aligns to 28:265/341 of P07913

query
sites
P07913

V

L

I

L

L

I

K

K

V

I

V

R

S

K

T

T

N

A

I

I

C
|
C

G

G

S

T

D

D

Q

V

H

H

M

I

V

Y

R

N

-

W

-

D

-

E

-

W

G

S

R

Q

T

K

T

T

A

I

Q

P

V

V

G

P

L

M

V

V

L

V

G

G

H

H

E

E

I

Y

T

V

G

G

E

E

I

V

V

V

E

G

K

I

G

G

R

Q

D

E

V

V

E

K

R

G

M

F

Q

K

I

I

G

G

D

D

L

R

V

V

S

S

V

G

P

E

F

G

N

H

V

I

A

T

C

C

G

G

R

H

C

C

R

R

S

N

C

C

K

R

E

G

M

G

H

R

T

T

G

H

V

L

C

C

L

R

T

N

V

T

N

-

P

-

A

-

R

-

A

-

G

-

A

-

Y

-

G

-

Y

-

V

I

D

G

M

V

G

G

-

V

D

N

W

R

T

P

G

G

G

C

Q

F

A

A

E

E

Y

Y

V

L

L

V

V

I

P

P

Y

A

A

-

D

-

F

F

N

N

L

A

L

F

K

K

L

I

P

P

E

D

R

N

D

I

K

S

A

-

M

-

E

-

K

-

I

-

R

D

D

D

L

L

T

A

C

A

L

I

S

F

D

D

I

P

L

F

P

G

T

N

G

A

Y

V

H

H

G

T

A

A

V

L

T

S

A

F

G

D

V

L

G

-

P

V

G

G

S

E

T

D

V

V

Y

L

V

V

A

S

G

G

A

A

G

G

P

P

V

I

G

G

L

I

A

M

A

A

S

V

A

A

R

K

L

H

L

V

G

G

A

A

A

R

C

N

V

V

I

V

V

I

G

T

D

D

L

V

N

N

P

E

A

Y

R

R

L

L

A

E

H

L

A

A

K

R

S

K

Q

M

G

G

F

I

-

T

E

R

V

A

V

V

D

N

L

V

S

A

K

K

D

-

T

-

P

-

L

-

H

-

E

E

Q

N

I

L

V

N

D

D

I

V

L

M

G

A

E

E

P

L

E

G

V

M

D

T

C

E

A

G

V

F

D

D

A

-

V

V

G

G

F

L

E

E

A

M

R

S

G

G

H

-

G

-

H

-

E

-

G

-

A

-

K

A

H

P

E

P

A

A

P

F

A

R

T

T

V

M

L

L

N

D

S

T

L

M

M

N

Q

H

V

G

T

G

R

R

V

I

A

A

G

-

N

M

I

L

G

G

I

I

P

P

C38 (= C47) mutation to D: Shows only 1% of wild-type catalytic activity. This mutant can be stimulated to the wild-type activity level after incubation with Zn(+).; mutation to S: Loss of catalytic activity. This mutant cannot be stimulated to the wild-type activity level after incubation with Zn(+).

5kiaA Crystal structure of l-threonine 3-dehydrogenase from burkholderia thailandensis
32% identity, 62% coverage: 23:269/399 of query aligns to 10:239/339 of 5kiaA

query
sites
5kiaA

P

P

K

G

M

L

Q

T

D

L

P

T

R

R

G

V

K

K

I

P

E

E

H

V

G

G

-

H

-

N

-

D

V

V

I

L

L

I

K

K

V

I

V

R

S

R

T

T

N

A

I

I

C
|
C

G
|
G

S
x
T

D

D

Q

I

H
|
H

M

I

V

W

R

K

G

W

R

D

T

D

T

W

A

A

Q

Q

V

K

G

T

-

I

-

P

-

V

L

M

V

H

L

V

G

G

H
|
H

E
|
E

I

Y

T

V

G

G

E

E

I

I

V

V

E

E

K

M

G

G

R

Q

D

E

V

V

E

R

R

G

M

F

Q

S

I

I

G

G

D

D

L

R

V

V

S

S

V

G

P

E

F

G

N

H

V

I

A

T

C
|
C

G

G

R

F

C
|
C

R

R

S

N

C
|
C

K

R

E

A

M

G

H

R

T

R

G

H

V

L

C
|
C

L

R

T

N

V

T

N

-

P

-

A

-

R

-

A

-

G

-

A

-

Y

-

G

-

Y

-

V
|
V

D

G

M

V

G

G

-

V

D

N

W

R

T

E

G

G

G

A

Q

F

A

A

E

E

Y

Y

V

L

L

A

V

I

P

P

Y

A

A

-

D

-

F

F

N

N

L

A

L

F

K

K

L

I

P

P

E

P

R

-

D

-

K

-

A

-

M

-

E

E

K

I

I

S

R

D

D

D

L

L

T

A

C

A

L

I

S

F

D
|
D

I
x
P

L

F

P

G

T
x
N

G
x
A

Y

T

H

H

G

T

A

A

V

L

T

S

A

F

G

N

V

L

G

-

P

V

G

G

S

E

T

D

V

V

Y

L

V

I

A

T

G

G

A

A

G

G

P

P

V

I

G

G

L

V

A

M

A

A

A

V

A

A

S

I

A

A

R

K

L

H

L

V

G

G

A

A

A

R

C

N

V

V

I

V

V

I

G

T

D

D

L

I

N

N

P

D

A

Y

R

R

L

L

A

D

H

L

A

A

K

R

S

R

Q

M

G

G

-

A

F

T

E

R

V

A

V

V

D

N

L

V

S

S

K

R

D

-

T

-

P

-

L

-

H

-

E

E

Q

S

I

L

V

R

D

D

I

V

L

M

G

A

E

D

P

L

E

H

V

M

D

T

C

E

A

G

V

F

D

D

A

-

V

V

G

G

F

L

E

E

A

M

R

S

G

G

active site: C37 (= C47), G38 (= G48), T39 (≠ S49), H42 (= H52), H61 (= H68), E62 (= E69), C91 (= C98), C94 (= C101), C97 (= C104), C105 (= C112), V109 (= V127), P147 (≠ I171), A151 (≠ G175)
binding calcium ion: D146 (= D170), N150 (≠ T174)
binding zinc ion: C91 (= C98), C94 (= C101), C97 (= C104), C105 (= C112)

Sites not aligning to the query:

active site: 333
binding calcium ion: 288

3gfbA L-threonine dehydrogenase (tktdh) from the hyperthermophilic archaeon thermococcus kodakaraensis (see paper)
29% identity, 90% coverage: 37:394/399 of query aligns to 30:347/347 of 3gfbA

query
sites
3gfbA

V

V

I

L

L

I

K

K

V

V

V

L

S

A

T

T

N

S

I

I

C
|
C

G
|
G

S
x
T

D

D

Q

L

H
|
H

M

I

V

Y

R

E

G

W

R

N

T

E

T

W

A

A

Q

Q

V

S

G

R

L

I

-

K

-

P

-

Q

V

I

L

M

G

G

H
|
H

E
|
E

I

V

T

A

G

G

E

E

I

V

V

V

E

E

K

V

G

G

R

P

D

G

V

V

E

E

R

D

M

L

Q

Q

I

V

G

G

D

D

L

Y

V

I

S

S

V

V

P

E

F

T

N

H

V

I

A

V

C
|
C

G

G

R

K

C
|
C

R

Y

S

A

C
|
C

K

K

E

H

M

N

H

R

T

Y

G

H

V

V

C
|
C

L

Q

T

N

V

T

N
x
K

P

-

A

-

R

-

A

-

G

-

A

I

Y

F

G

G

Y

-

V

V

D

D

M

M

G

-

D

-

W

-

T

D

G

G

G

V

Q

F

A

A

E

H

Y

Y

V

A

L

I

V

V

P

P

Y

A

A

K

D

-

F

-

N

N

L

A

L

W

K

K

L

N

P

P

E

-

R

K

D

D

K

M

A

P

M

P

E

E

K

Y

I

A

R

A

D

L

L

Q

T

E

C
x
P

L

L

S

G

D

N

I
x
A

L

V

P

D

T

T

G

-

Y

-

H

-

G

-

A

-

V

V

T

L

A

A

G

G

V

P

G

I

P

A

G

G

S

R

T

S

V

T

Y

L

V

I

A

T

G
|
G

A

A

G
|
G

P
|
P

V
x
L

G

G

L

L

A

L

A

G

A

I

A

A

S

V

A

A

R

K

L

A

L

S

G

G

A

A

A

Y

C

P

V

V

I

I

V

V

G
x
S

D
x
E

L
x
P

N

S

P

E

A

F

R
|
R

L

R

A

K

H

L

A

A

K

K

S

K

Q

V

G

G

F

A

E

D

-

Y

V

V

D

N

L

P

S

F

K

E

D

E

T

D

P

P

L

V

H

-

E

K

Q

F

I

V

V

M

D

D

I

I

L

-

G

-

E

-

P

-

E

-

V

T

D

D

C

G

A

A

V

G

D

V

A

E

V

V

G

F

F

L

E

E

A
x
F

R
x
S

G

G

H

-

G

-

H

-

E

-

G

-

A

-

K

-

H

-

E

-

A
|
A

P

P

A

K

T

A

V

L

L

E

N

Q

S

G

L

L

M

K

Q

A

V

V

T

T

R

P

V

-

A

G

G

G

N

R

I

V

G

S

I

L

P
x
L

G
|
G

L
|
L

Y

F

V

P

T

R

E

E

D

V

P

T

G

I

A

D

V

F

D

N

A

N

A

L

A

I

K

I

I

F

G

K

A

A

L

L

S

E

I

V

R

H

F

-

G

-

L

-

G

G

W
x
I

A
x
T

K

G

S

R

H

H

S

L

F

W

H

E

T

T

G

W

Q

Y

T

T

P

V

T

S

M

-

K

-

Y

-

N

-

R

-

Q

S

L

L

M

I

Q

Q

A

S

I

-

M

-

W

-

D

G

R

K

I

L

N

N

I

L

A

D

E

P

V

I

G

T

V

H

Q

K

V

Y

I

K

N

G

L

F

D

D

Q

K

A

F

P

E

E

E

G

A

Y

F

G

E

E

L

F

M

D

R

A

A

G

G

V

K

P

T

K

G

K
|
K

F

V

V

V

I

F

D

F

P

P

H

H

K

K

active site: C40 (= C47), G41 (= G48), T42 (≠ S49), H45 (= H52), H65 (= H68), E66 (= E69), C95 (= C98), C98 (= C101), C101 (= C104), C109 (= C112), K113 (≠ N116), P151 (≠ C167), A155 (≠ I171), K340 (= K387)
binding nicotinamide-adenine-dinucleotide: G173 (= G194), G175 (= G196), P176 (= P197), L177 (≠ V198), S196 (≠ G217), E197 (≠ D218), P198 (≠ L219), R202 (= R223), F241 (≠ A267), S242 (≠ R268), A244 (= A279), L264 (≠ P300), G265 (= G301), L266 (= L302), I289 (≠ W328), T290 (≠ A329)

Q5JI69 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) (see paper)
29% identity, 90% coverage: 37:394/399 of query aligns to 32:349/350 of Q5JI69

query
sites
Q5JI69

V

V

I

L

L

I

K

K

V

V

V

L

S

A

T

T

N

S

I

I

C

C

G

G

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T

D

D

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L

H

H

M

I

V

Y

R

E

G

W

R

N

T

E

T

W

A

A

Q

Q

V

S

G

R

L

I

-

K

-

P

-

Q

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I

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M

G

G

H

H

E

E

I

V

T

A

G

G

E

E

I

V

V

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E

K

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G

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R

P

D

G

V

V

E

E

R

D

M

L

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Q

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V

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G

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D

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Y

V

I

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S

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V

P

E

F

T

N

H

V

I

A

V

C

C

G

G

R

K

C

C

R

Y

S

A

C

C

K

K

E

H

M

N

H

R

T

Y

G

H

V

V

C

C

L

Q

T

N

V

T

N

K

P

-

A

-

R

-

A

-

G

-

A

I

Y

F

G

G

Y

-

V

V

D

D

M

M

G

-

D

-

W

-

T

D

G

G

G

V

Q

F

A

A

E

H

Y

Y

V

A

L

I

V

V

P

P

Y

A

A

K

D

-

F

-

N

N

L

A

L

W

K

K

L

N

P

P

E

-

R

K

D

D

K

M

A

P

M

P

E

E

K

Y

I

A

R

A

D

L

L

Q

T

E

C

P

L

L

S

G

D

N

I

A

L

V

P

D

T

T

G

-

Y

-

H

-

G

-

A

-

V

V

T

L

A

A

G

G

V

P

G

I

P

A

G

G

S

R

T

S

V

T

Y

L

V

I

A

T

G

G

A

A

G

G

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P

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x
L

G

G

L

L

A

L

A

G

A

I

A

A

S

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A

A

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K

L

A

L

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G

A

A

A

Y

C

P

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I

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x
E

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P

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S

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E

A

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|
R

L

R

A

K

H

L

A

A

K

K

S

K

Q

V

G

G

F

A

E

D

-

Y

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L

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S

F

K

E

D

E

T

D

P

P

L

V

H

-

E

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Q

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I

V

V

M

D

D

I

I

L

-

G

-

E

-

P

-

E

-

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T

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D

C

G

A

A

V

G

D

V

A

E

V

V

G

F

F

L

E

E

A

F

R

S

G

G

H

-

G

-

H

-

E

-

G

-

A

-

K

-

H

-

E

-

A

A

P

P

A

K

T

A

V

L

L

E

N

Q

S

G

L

L

M

K

Q

A

V

V

T

T

R

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V

-

A

G

G

G

N

R

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V

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S

I

L

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x
L

G
|
G

L
|
L

Y

F

V

P

T

R

E

E

D

V

P

T

G

I

A

D

V

F

D

N

A

N

A

L

A

I

K

I

I

F

G

K

A

A

L

L

S

E

I

V

R

H

F

-

G

-

L

-

G

G

W
x
I

A
x
T

K

G

S

R

H

H

S

L

F

W

H

E

T

T

G

W

Q

Y

T

T

P

V

T

S

M

-

K

-

Y

-

N

-

R

-

Q

S

L

L

M

I

Q

Q

A

S

I

-

M

-

W

-

D

G

R

K

I

L

N

N

I

L

A

D

E

P

V

I

G

T

V

H

Q

K

V

Y

I

K

N

G

L

F

D

D

Q

K

A

F

P

E

E

E

G

A

Y

F

G

E

E

L

F

M

D

R

A

A

G

G

V

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P

T

K

G

K

K

F

V

V

V

I

F

D

F

P

P

H

H

K

K

L179 (≠ V198) binding
E199 (≠ D218) binding
R204 (= R223) binding
LGL 266:268 (≠ PGL 300:302) binding
IT 291:292 (≠ WA 328:329) binding

3fplA Chimera of alcohol dehydrogenase by exchange of the cofactor binding domain res 153-295 of c. Beijerinckii adh by t. Brockii adh (see paper)
35% identity, 57% coverage: 38:264/399 of query aligns to 28:244/351 of 3fplA

query
sites
3fplA

I

I

L

V

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R

V

P

V

L

S

A

T

V

N

S

I

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C
|
C

G
x
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S
|
S

D

D

Q

I

H
|
H

M

T

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V

-

F

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E

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G

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A

T

L

T

G

A

D

Q

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G

N

L

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L

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G

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H
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H

E
|
E

I

A

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V

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G

E

E

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E

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V

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G

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G

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D

L

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C

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T

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T

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P

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x
D

G

W

R

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C
x
S

R

L

S

-

C

-

K

-

E

E

M
x
V

H

Q

T

A

G

G

V

F

C

Q

L

Q

T

H

V
x
S

N

N

P

G

A

M

R

L

A

A

G

G

G

-

A

-

Y

W

G

K

Y

F

V

S

D

N

M

F

G

K

D

D

W

G

T

V

G

F

G

G

Q

-

A

-

E

E

Y

Y

V

F

L

H

V

V

P

N

Y

D

A

A

D

D

F

M

N

N

L

L

L

A

K

I

L

L

P

P

E

-

R

K

D

D

K

M

A

P

M

L

E

E

K

-

I

-

R

-

D

N

L

A

T

V

C

M

L

I

S

T

D
|
D

I

M

L

M

P

T

T
|
T

G

G

Y

F

H

H

G

G

A

A

V

E

T

L

A

A

G

D

V

I

G

E

P

L

G

G

S

A

T

T

V

V

Y

A

V

V

A

L

G

G

A

I

G

G

P

P

V

V

G

G

L

L

A

M

A

A

A

V

A

A

S

G

A

A

R

K

L

L

L

R

G

G

A

A

A

G

C

R

V

I

I

I

V

A

G

V

D

G

L

S

N

R

P

P

A

V

R

C

L

V

A

D

H

A

A

A

K

K

S

Y

Q

Y

G

G

F

A

E

T

V

D

V

I

D

V

L

N

S

Y

K

K

D

D

T

G

P

P

L

I

H

E

E

S

Q

Q

I

I

V

M

D

N

I

L

L

T

G

E

E

G

P

K

E

G

V

V

D

D

C

A

A

A

V

I

D

I

A

A

V

G

G

G

active site: C37 (= C47), T38 (≠ G48), S39 (= S49), H42 (= H52), H59 (= H68), E60 (= E69), D89 (≠ C98), S92 (≠ C101), V95 (≠ M107), S103 (≠ V115), D150 (= D170), T154 (= T174)
binding zinc ion: C37 (= C47), H59 (= H68), D150 (= D170)

Sites not aligning to the query:

active site: 346

2dfvA Hyperthermophilic threonine dehydrogenase from pyrococcus horikoshii (see paper)
28% identity, 89% coverage: 37:390/399 of query aligns to 30:343/346 of 2dfvA

query
sites
2dfvA

V

V

I

L

L

I

K

K

V

V

V

L

S

A

T

T

N

S

I

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C

G
|
G

S
x
T

D

D

Q

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H
|
H

M

I

V

Y

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E

G

W

R

N

T

E

T

W

A

A

Q

Q

V

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G

R

L

I

-

K

-

P

-

Q

V

I

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M

G

G

H
|
H

E
|
E

I

V

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A

G

G

E

E

I

V

V

V

E

E

K

I

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E

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G

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D

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T

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H

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C

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C
|
C

R

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S

A

C
|
C

K

R

E

R

M

G

H

Q

T

Y

G

H

V

V

C
|
C

L

Q

T

N

V

T

N
x
K

P

-

A

-

R

-

A

-

G

-

A

I

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F

G

G

Y

V

V

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D

-

M

-

G

-

D

D

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T

D

G

G

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A

A

E

E

Y

Y

V

A

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P

Y

A

A

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D

-

F

-

N

N

L

I

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K

L

N

P

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S

D

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K

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A

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M

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E

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I

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R

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T

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E

I
x
P

L

L

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N

G
x
A

Y

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A

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A

A

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G

S

K

T

S

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V

I

A

T

G

G

A

A

G
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G

P
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P

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x
L

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L

A

L

A

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A

I

A

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G

A

A

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E

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x
P

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R

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A

A

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K

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K

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G

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A

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D

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E

G

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V

D

D

C

V

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V

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D

E

A
x
F

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x
S

G

G

F

-

E

-

A

-

R

-

G

-

H

-

G

-

H

-

E

-

G

-

A

-

K

-

H

-

E

-

A
|
A

P

P

A

K

T

A

V

L

L

E

N

Q

S

G

L

L

M

Q

Q

A

V

V

T

T

R

P

V

-

A

A

G

G

N

R

I

V

G

S

I

L

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x
L

G
|
G

L
|
L

Y

Y

V

-

T

-

E

-

D

-

P

P

G

G

A

K

V

V

-

T

-

I

-

D

-

F

D

N

A

N

A

L

A

I

K

I

I

F

G

K

A

A

L

L

S

T

I

I

R

-

F

-

G

-

L

Y

G

G

W
x
I

A
x
T

K

G

S

R

H

H

S

L

F

W

H

E

T

T

G

W

Q

Y

T

T

P

V

T

S

M

-

K

-

Y

-

N

-

R

-

Q

R

L

L

M

L

Q

Q

A

S

I

-

M

-

W

-

D

G

R

K

I

L

N

N

I

L

A

D

E

P

V

I

G

T

V

H

Q

K

V

Y

I

K

N

G

L

F

D

D

Q

K

A

Y

P

E

E

E

G

A

Y

F

G

E

E

L

F

M

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A

A

G

G

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P

T

K

G

K
|
K

F

V

V

V

I

F

active site: C40 (= C47), G41 (= G48), T42 (≠ S49), H45 (= H52), H65 (= H68), E66 (= E69), C95 (= C98), C98 (= C101), C101 (= C104), C109 (= C112), K113 (≠ N116), P151 (≠ I171), A155 (≠ G175), K340 (= K387)
binding nicotinamide-adenine-dinucleotide: G175 (= G196), P176 (= P197), L177 (≠ V198), E197 (≠ D218), P198 (≠ L219), R202 (= R223), F241 (≠ A262), S242 (≠ V263), A244 (= A279), L264 (≠ P300), G265 (= G301), L266 (= L302), I289 (≠ W328), T290 (≠ A329)
binding zinc ion: C95 (= C98), C101 (= C104), C109 (= C112)

O58389 L-threonine 3-dehydrogenase; L-ThrDH; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 2 papers)
28% identity, 89% coverage: 37:390/399 of query aligns to 32:345/348 of O58389

query
sites
O58389

V

V

I

L

L

I

K

K

V

V

V

L

S

A

T

T

N

S

I

I

C
|
C

G

G

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x
T

D

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M

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R

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R

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A

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I

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K

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H

E
|
E

I

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A

G

G

E

E

I

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E

K

I

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G

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D

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V

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E

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N

H

V

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A

V

C
|
C

G

G

R

K

C
|
C

R

Y

S

A

C
|
C

K

R

E

R

M

G

H

Q

T

Y

G

H

V

V

C
|
C

L

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T

N

V

T

N

K

P

-

A

-

R

-

A

-

G

-

A

I

Y

F

G

G

Y

V

V

-

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-

M

-

G

-

D

D

W

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D

G

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A

A

E

E

Y

Y

V

A

L

V

V

V

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P

Y

A

A

Q

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-

F

-

N

N

L

I

L

W

K

K

L

N

P

P

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R

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D

I

K

P

A

P

M

-

E

-

K

-

I

-

R

-

D

E

L

Y

T

A

C

T

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L

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x
E

I

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L

L

P

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T

N

G

A

Y

V

H

D

G

-

A

T

V

V

T

L

A

A

G

G

V

P

G

I

P

S

G

G

S

K

T

S

V

V

Y

L

V

I

A

T

G

G

A

A

G

G

P

P

V
x
L

G

G

L

L

A

L

A

G

A

I

A

A

S

V

A

A

R

K

L

A

L

S

G

G

A

A

A

Y

C

P

V

V

I

I

V

V

G

S

D
x
E

L

P

N

S

P

D

A

F

R
|
R

L

R

A

E

H

L

A

A

K

K

S

K

Q

V

G

G

F

A

E

D

V

Y

V

V

D

I

L

N

S

P

K

F

D

E

T

E

P

D

L

V

H

V

E

K

Q

E

I

V

V

M

D

D

I

I

L

T

G

D

E

G

P

N

E

G

V

V

D

D

C

V

A

F

V

L

D

E

A

F

V

S

G

G

F

-

E

-

A

-

R

-

G

-

H

-

G

-

H

-

E

-

G

-

A

-

K

-

H

-

E

-

A

A

P

P

A

K

T

A

V

L

L

E

N

Q

S

G

L

L

M

Q

Q

A

V

V

T

T

R

P

V

-

A

A

G

G

N

R

I

V

G

S

I

L

P
x
L

G
|
G

L
|
L

Y

Y

V

-

T

-

E

-

D

-

P

P

G

G

A

K

V

V

-

T

-

I

-

D

-

F

D

N

A

N

A

L

A

I

K

I

I

F

G

K

A

A

L

L

S

T

I

I

R

-

F

-

G

-

L

Y

G

G

W
x
I

A
x
T

K

G

S
x
R

H

H

S

L

F

W

H

E

T

T

G

W

Q

Y

T

T

P

V

T

S

M

-

K

-

Y

-

N

-

R

-

Q

R

L

L

M

L

Q

Q

A

S

I

-

M

-

W

-

D

G

R

K

I

L

N

N

I

L

A

D

E

P

V

I

G

T

V

H

Q

K

V

Y

I

K

N

G

L

F

D

D

Q

K

A

Y

P

E

E

E

G

A

Y

F

G

E

E

L

F

M

D

R

A

A

G

G

V

K

P

T

K

G

K

K

F

V

V

V

I

F

C42 (= C47) binding
T44 (≠ S49) mutation to A: Total loss of enzymatic activity.
H67 (= H68) binding
E68 (= E69) binding
C97 (= C98) binding
C100 (= C101) binding
C103 (= C104) binding
C111 (= C112) binding
E152 (≠ D170) mutation E->A,Q: Almost complete loss of enzymatic activity.; mutation to C: 120-fold decrease in catalytic efficiency.; mutation to D: Shows 3-fold higher turnover rate and reduced affinities toward L-threonine and NAD(+), compared to wild-type.; mutation to K: Total loss of enzymatic activity.
L179 (≠ V198) binding
E199 (≠ D218) binding ; mutation to A: Large decrease in affinity for NAD(+).
R204 (= R223) binding ; mutation to A: Large decrease in affinity for NAD(+).
LGL 266:268 (≠ PGL 300:302) binding
IT 291:292 (≠ WA 328:329) binding
R294 (≠ S331) mutation to A: 4000-fold decrease in catalytic efficiency.

7y9pA Xylitol dehydrogenase s96c/s99c/y102c mutant(thermostabilized form) from pichia stipitis (see paper)
30% identity, 66% coverage: 2:263/399 of query aligns to 1:260/357 of 7y9pA

query
sites
7y9pA

S

T

G

A

N

N

R

P

G

S

V

L

V

V

Y

L

L

N

G

K

A

I

G

D

K

D

V

I

E

S

V

F

Q

E

K

T

I

Y

D

D

Y

A

P

P

K

E

M

I

Q

S

D

E

P

P

R

T

G

D

K

-

I

-

E

-

H

-

G

-

V

V

I

L

L

V

K

Q

V

V

V

K

S

K

T

T

N

G

I

I

C
|
C

G

G

S

S

D

D

Q

I

H

H

M

F

V

Y

R

A

-

H

-

G

-

R

-

I

G

G

R

N

T

F

T

V

A

L

Q

T

V

K

G

P

L

M

V

V

L

L

G

G

H
|
H

E
|
E

I

S

T

A

G

G

E

T

I

V

V

V

E

Q

K

V

G

G

R

K

D

G

V

V

E

T

R

S

M

L

Q

K

I

V

G

G

D

D

L

N

V

V

S

A

V

I

P

E

F

P

N

G

V

I

A

P

C
|
C

G

R

R

F

C
|
C

R

D

S

E

C
|
C

K

K

E

S

M

G

H

H

T

Y

G

N

V

L

C
|
C

L

-

T

-

V

-

N

-

P

P

A

H

R

M

A

A

G

F

G

A

A

A

Y

T

G

P

Y

-

V

-

D

N

M

E

G

P

D

N

W

P

T

P

G

G

G

T

Q

L

A

C

E

K

Y

Y

V

F

L

K

V

S

P

P

Y

-

A

E

D

D

F

F

N

-

L

L

L

V

K

K

L

L

P

P

E

D

R

H

D

V

K

S

A

-

M

-

E

-

K

-

I

-

R

L

D

E

L

L

T

G

C

A

L

L

S

V

D

E

I

P

L

L

P

S

T

V

G

G

Y

V

H

H

G

A

A

S

V

K

T

L

A

G

G

S

V

V

G

A

P

F

G

G

S

D

T

Y

V

V

Y

A

V

V

A

F

G

G

A

A

G

G

P

P

V

V

G

G

L

L

A

L

A

A

S

V

A

A

R

K

L

T

L

F

G

G

A

A

A

K

C

G

V

V

I

I

V

V

G

V

D

D

L

I

N

F

P

D

A

N

R

K

L

L

A

K

H

M

A

A

K

K

S

D

Q

I

G

G

F

A

E

A

V

T

V

H

D

T

L

F

S

N

K

S

D

K

T

T

P

G

L

G

H

S

E

E

Q

E

I

L

V

I

D

K

I

A

L

F

G

G

-

G

-

N

-

V

-

P

-

N

-

V

-

L

-

E

-

C

-

T

-

G

-

A

E

E

P

P

E

C

V

I

D

K

C

L

A

G

V

V

D

D

A

A

V

I

binding zinc ion: C40 (= C47), H65 (= H68), E66 (= E69), C95 (= C98), C98 (= C101), C101 (= C104), C109 (= C112)

7xy9A Cryo-em structure of secondary alcohol dehydrogenases tbsadh after carrier-free immobilization based on weak intermolecular interactions
33% identity, 57% coverage: 38:264/399 of query aligns to 29:245/344 of 7xy9A

query
sites
7xy9A

I

I

L

V

K

R

V

P

V

L

S

A

T

V

N

A

I

P

C
|
C

G

T

S

S

D

D

Q

I

H

H

M

T

V

V

-

F

R

E

G

G

R

A

T

I

T

G

A

E

Q

R

V

H

G

N

L

M

V

I

L

L

G

G

H
|
H

E
|
E

I

A

T

V

G

G

E

E

I

V

V

V

E

E

K

V

G

G

R

S

D

E

V

V

E

K

R

D

M

F

Q

K

I

P

G

G

D

D

L

R

V

V

S

V

V

V

P

P

F

A

N

N

V

T

A

P

C

D

G

W

R

R

C

T

R

S

S

E

C

V

K

Q

E

R

-

G

-

Y

-
x
H

M

Q

H
|
H

T

S

G

G

V

G

C

M

L

L

T

-

V

-

N

-

P

-

A

-

R

-

A

A

G

G

G

W

A

K

Y

F

G

S

Y

N

V

V

D

K

M

D

G

G

D

V

W

F

T

-

G

-

Q

-

A

G

E

E

Y

F

V

F

L

H

V

V

P

N

Y

D

A

A

D

D

F

M

N

N

L

L

L

A

K

H

L

L

P

P

E

K

R

E

D

I

K

P

A

-

M

-

E

-

K

-

I

L

R

E

D

A

L

A

T

V

C

M

L

I

S

P

D
|
D

I

M

L

M

P

T

T

T

G

G

Y

F

H
|
H

G

G

A

A

V

E

T

L

A

A

G

D

V

I

G

E

P

L

G

G

S

A

T

T

V

V

Y

A

V

V

A

L

G

G

A

I

G

G

P

P

V

V

G

G

L

L

A

M

A

A

A

V

A

A

S

G

A

A

R

K

L

L

L

R

G

G

A

A

A

G

C

R

V

I

I

I

V

A

G

V

D

G

L

S

N

R

P

P

A

V

R

C

L

V

A

D

H

A

A

A

K

K

S

Y

Q

Y

G

G

F

A

E

T

V

D

V

I

D

V

L

N

S

Y

K

K

D

D

T

G

P

P

L

I

H

E

E

S

Q

Q

I

I

V

M

D

N

I

L

L

T

G

E

E

G

P

K

E

G

V

V

D

D

C

A

A

A

V

I

D

I

A

A

V

G

G

G

binding magnesium ion: H101 (vs. gap), H103 (= H108), H158 (= H177)
binding zinc ion: C38 (= C47), H60 (= H68), E61 (= E69), D151 (= D170)

Sites not aligning to the query:

binding magnesium ion: 288, 290, 291, 293, 294

2ejvA Crystal structure of threonine 3-dehydrogenase complexed with NAD+
32% identity, 54% coverage: 37:251/399 of query aligns to 28:224/343 of 2ejvA

query
sites
2ejvA

V

I

I

L

L

V

K

R

V

V

V

E

S

A

T

A

N

S

I

I

C
|
C

G
|
G

S
x
T

D

D

Q

L

H
|
H

M

I

-

W

-

K

-

W

-

D

-

A

-

W

V

A

R

R

G

G

R

R

T

I

T

R

A

P

Q

P

V

-

G

-

L

L

V

V

L

T

G

G

H
|
H

E
|
E

I

F

T

S

G

G

E

V

I

V

V

E

E

A

K

V

G

G

R

P

D

G

V

V

E

R

R

R

M

P

Q

Q

I

V

G

G

D

D

L

H

V

V

S

S

V

L

P

E

F

S

N

H

V

I

A

V

C
|
C

G

H

R

A

C
|
C

R

P

S

A

C
|
C

K

R

E

T

M

G

H

N

T

Y

G

H

V

V

C
|
C

L

L

T

N

V

T

N
x
Q

P

-

A

-

R

-

A

-

G

-

A

-

Y

-

G

-

Y

-

V

I

D

L

M

G

G

V

D

D

W

R

T

D

G

G

Q

F

A

A

E

E

Y

Y

V

V

L

V

V

V

P

P

Y

A

A

E

D

N

F

-

N

-

L

-

L

A

K

W

L

V

P

N

E

P

R

K

D

D

K

L

A

P

M

F

E

E

K

V

I

A

R

A

D

I

L

L

T

E

C
x
P

L

F

S

G

D

N

I
x
A

L

V

P

H

T

T

G

V

Y

Y

H

A

G

G

A

S

V

-

T

-

A

-

G

G

V

V

G

S

P

-

G

G

S

K

T

S

V

V

Y

L

V

I

A

T

G
|
G

A

A

G
|
G

P
|
P

V
x
I

G

G

L

L

A

M

A

A

A

M

S

V

A

V

R

R

L

A

L

S

G

G

A

A

A

G

C

P

V

I

I

L

V

V

G
x
S

D
|
D

L
x
P

N

N

P

P

A

Y

R
|
R

L

L

A

A

H

F

A

A

K

R

S

P

Q

Y

G

A

F

D

E

R

V

L

V

V

D

N

L

-

S

-

K

-

D

-

T

-

P

P

L

L

H

E

E

E

Q

D

I

L

V

L

D

E

I

V

L

V

active site: C38 (= C47), G39 (= G48), T40 (≠ S49), H43 (= H52), H63 (= H68), E64 (= E69), C93 (= C98), C96 (= C101), C99 (= C104), C107 (= C112), Q111 (≠ N116), P149 (≠ C167), A153 (≠ I171)
binding nicotinamide-adenine-dinucleotide: G172 (= G194), G174 (= G196), P175 (= P197), I176 (≠ V198), S195 (≠ G217), D196 (= D218), P197 (≠ L219), R201 (= R223)
binding zinc ion: C38 (= C47), H63 (= H68), E64 (= E69), C96 (= C101), C99 (= C104), C107 (= C112)

Sites not aligning to the query:

active site: 336
binding nicotinamide-adenine-dinucleotide: 238, 239, 241, 244, 261, 262, 263

2dq4A Crystal structure of threonine 3-dehydrogenase
32% identity, 54% coverage: 37:251/399 of query aligns to 28:224/343 of 2dq4A

query
sites
2dq4A

V

I

I

L

L

V

K

R

V

V

V

E

S

A

T

A

N

S

I

I

C
|
C

G
|
G

S
x
T

D

D

Q

L

H
|
H

M

I

-

W

-

K

-

W

-

D

-

A

-

W

V

A

R

R

G

G

R

R

T

I

T

R

A

P

Q

P

V

-

G

-

L

L

V

V

L

T

G

G

H
|
H

E
|
E

I

F

T

S

G

G

E

V

I

V

V

E

E

A

K

V

G

G

R

P

D

G

V

V

E

R

R

R

M

P

Q

Q

I

V

G

G

D

D

L

H

V

V

S

S

V

L

P

E

F

S

N

H

V

I

A

V

C
|
C

G

H

R

A

C
|
C

R

P

S

A

C
|
C

K

R

E

T

M

G

H

N

T

Y

G

H

V

V

C
|
C

L

L

T

N

V

T

N
x
Q

P

-

A

-

R

-

A

-

G

-

A

-

Y

-

G

-

Y

-

V

I

D

L

M

G

G

V

D

D

W

R

T

D

G

G

Q

F

A

A

E

E

Y

Y

V

V

L

V

V

V

P

P

Y

A

A

E

D

N

F

-

N

-

L

-

L

A

K

W

L

V

P

N

E

P

R

K

D

D

K

L

A

P

M

F

E

E

K

V

I

A

R

A

D

I

L

L

T

E

C
x
P

L

F

S

G

D

N

I
x
A

L

V

P

H

T

T

G

V

Y

Y

H

A

G

G

A

S

V

-

T

-

A

-

G

G

V

V

G

S

P

-

G

G

S

K

T

S

V

V

Y

L

V

I

A

T

G

G

A

A

G

G

P

P

V

I

G

G

L

L

A

M

A

A

A

M

S

V

A

V

R

R

L

A

L

S

G

G

A

A

A

G

C

P

V

I

I

L

V

V

G

S

D

D

L

P

N

N

P

P

A

Y

R

R

L

L

A

A

H

F

A

A

K

R

S

P

Q

Y

G

A

F

D

E

R

V

L

V

V

D

N

L

-

S

-

K

-

D

-

T

-

P

P

L

L

H

E

E

E

Q

D

I

L

V

L

D

E

I

V

L

V

active site: C38 (= C47), G39 (= G48), T40 (≠ S49), H43 (= H52), H63 (= H68), E64 (= E69), C93 (= C98), C96 (= C101), C99 (= C104), C107 (= C112), Q111 (≠ N116), P149 (≠ C167), A153 (≠ I171)
binding zinc ion: C38 (= C47), H63 (= H68), E64 (= E69), C93 (= C98), C96 (= C101), C107 (= C112)

Sites not aligning to the query:

active site: 336

Q5SKS4 L-threonine 3-dehydrogenase; TDH; EC 1.1.1.103 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
32% identity, 54% coverage: 37:251/399 of query aligns to 28:224/343 of Q5SKS4

query
sites
Q5SKS4

V

I

I

L

L

V

K

R

V

V

V

E

S

A

T

A

N

S

I

I

C
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C

G

G

S

T

D

D

Q

L

H

H

M

I

-

W

-

K

-

W

-

D

-

A

-

W

V

A

R

R

G

G

R

R

T

I

T

R

A

P

Q

P

V

-

G

-

L

L

V

V

L

T

G

G

H
|
H

E
|
E

I

F

T

S

G

G

E

V

I

V

V

E

E

A

K

V

G

G

R

P

D

G

V

V

E

R

R

R

M

P

Q

Q

I

V

G

G

D

D

L

H

V

V

S

S

V

L

P

E

F

S

N

H

V

I

A

V

C
|
C

G

H

R

A

C
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C

R

P

S

A

C
|
C

K

R

E

T

M

G

H

N

T

Y

G

H

V

V

C
|
C

L

L

T

N

V

T

N

Q

P

-

A

-

R

-

A

-

G

-

A

-

Y

-

G

-

Y

-

V

I

D

L

M

G

G

V

D

D

W

R

T

D

G

G

Q

F

A

A

E

E

Y

Y

V

V

L

V

V

V

P

P

Y

A

A

E

D

N

F

-

N

-

L

-

L

A

K

W

L

V

P

N

E

P

R

K

D

D

K

L

A

P

M

F

E

E

K

V

I

A

R

A

D

I

L

L

T

E

C

P

L

F

S

G

D

N

I

A

L

V

P

H

T

T

G

V

Y

Y

H

A

G

G

A

S

V

-

T

-

A

-

G

G

V

V

G

S

P

-

G

G

S

K

T

S

V

V

Y

L

V

I

A

T

G

G

A

A

G

G

P

P

V
x
I

G

G

L

L

A

M

A

A

A

M

S

V

A

V

R

R

L

A

L

S

G

G

A

A

A

G

C

P

V

I

I

L

V

V

G

S

D
|
D

L

P

N

N

P

P

A

Y

R
|
R

L

L

A

A

H

F

A

A

K

R

S

P

Q

Y

G

A

F

D

E

R

V

L

V

V

D

N

L

-

S

-

K

-

D

-

T

-

P

P

L

L

H

E

E

E

Q

D

I

L

V

L

D

E

I

V

L

V

C38 (= C47) binding
H63 (= H68) binding
E64 (= E69) binding
C93 (= C98) binding
C96 (= C101) binding
C99 (= C104) binding
C107 (= C112) binding
I176 (≠ V198) binding
D196 (= D218) binding
R201 (= R223) binding

Sites not aligning to the query:

261:263 binding
286:288 binding

P25405 Alcohol dehydrogenase 1A; Alcohol dehydrogenase I-A; ADH IA; EC 1.1.1.1 from Saara hardwickii (Indian spiny-tailed lizard) (Uromastyx hardwickii) (see paper)
30% identity, 58% coverage: 35:264/399 of query aligns to 34:271/375 of P25405

query
sites
P25405

H

H

G

E

V

V

I

R

L

I

K

K

V

I

V

L

S

A

T

T

N

G

I

I

C

C

G

R

S

S

D

D

Q

D

H

H

M

V

V

I

R

S

G

G

R

A

T

F

T

K

A

M

Q

P

V

L

G

P

L

M

V

V

L

L

G

G

H

H

E

E

I

A

T

A

G

G

E

V

I

V

V

E

E

S

K

V

G

G

R

E

D

G

V

V

E

T

R

C

M

V

Q

K

I

P

G

G

D

D

L

K

V

V

S

I

V

P

P

L

F

F

N

V

V

P

A

Q

C

C

G

G

R

K

C

C

R

S

S

S

C

C

K

R

E

S

M

T

H

R

T

G

G

N

V

L

C

C

L

T

T

S

V

N

N

D

P

L

A

S

R

A

A

A

G

T

G

G

A

L

Y

-

G

-

Y

-

V

-

D

-

M

M

G

P

D

D

W

G

T

T

G

S

-

R

-

F

-

T

-

C

-

K

G

G

Q

K

A

S

E

L

Y

H

V

H

L

F

V

I

P

S

Y

T

A

S

D

S

F

F

N

T

L

E

L

Y

K

T

L

V

P

V

E

H

R

E

D

N

K

S

A

V

-

V

-

K

-

I

-

D

-

A

-

P

M

L

E

E

K

K

I

V

R

C

D

L

L

I

T

G

C

C

L

G

S

-

D

-

I

-

L

F

P

S

T

T

G

G

Y

Y

H

G

G

A

A

A

V

V

-

E

T

T

A

A

G

K

V

V

G

E

P

P

G

G

S

S

T

T

V

C

Y

A

V

V

A

F

G

G

A

L

G

G

P

G

V

V

G

G

L

L

A

S

A

A

A

V

A

M

S

G

A

C

R

K

L

A

G

G

A

A

A

S

C

R

V

I

I

I

V

G

G

V

D

D

L

I

N

N

P

K

A

D

R

K

L

F

A

P

H

K

A

A

K

K

S

E

Q

M

G

G

-

A

F

T

E

E

V

C

V

V

D

N

-

P

L

L

S

D

K

Y

D

K

T

K

P

P

L

I

H

N

E

E

Q

V

I

L

V

F

D

D

I

L

L

T

G

G

E

G

P

E

E

G

V

V

D

D

C

Y

A

S

V

F

D

E

A

V

V

I

G

G

Sites not aligning to the query:

1 modified: N-acetylglycine

Query Sequence

>PP_0328 FitnessBrowser__Putida:PP_0328
MSGNRGVVYLGAGKVEVQKIDYPKMQDPRGKKIEHGVILKVVSTNICGSDQHMVRGRTTA
QVGLVLGHEITGEIVEKGRDVERMQIGDLVSVPFNVACGRCRSCKEMHTGVCLTVNPARA
GGAYGYVDMGDWTGGQAEYVLVPYADFNLLKLPERDKAMEKIRDLTCLSDILPTGYHGAV
TAGVGPGSTVYVAGAGPVGLAAAASARLLGAACVIVGDLNPARLAHAKSQGFEVVDLSKD
TPLHEQIVDILGEPEVDCAVDAVGFEARGHGHEGAKHEAPATVLNSLMQVTRVAGNIGIP
GLYVTEDPGAVDAAAKIGALSIRFGLGWAKSHSFHTGQTPTMKYNRQLMQAIMWDRINIA
EVVGVQVINLDQAPEGYGEFDAGVPKKFVIDPHKMWGAA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory