SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PP_0406 FitnessBrowser__Putida:PP_0406 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q88QT2 N-acetylmuramate alpha-1-phosphate uridylyltransferase; MurNAc-1P uridylyltransferase; MurNAc-alpha-1P uridylyltransferase; EC 2.7.7.99 from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
100% identity, 100% coverage: 1:223/223 of query aligns to 1:223/223 of Q88QT2

query
sites
Q88QT2

M

M

K

K

A

A

M

M

I

I

L

L

A

A

G

G

K

K

G
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G

E
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E

R
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R

M

M

R

R

P

P

L

L

T

T

L

L

H

H

T

T

P

P

K
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K

P

P

L

L

V

V

P

P

V

V

A

A

G

G

Q

Q

P

P

L

L

I

I

E

E

Y

Y

H

H

L

L

R

R

A

A

L

L

A

A

G

G

V

V

T

T

E

E

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V

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I

N

N

H

H

A

A

W

W

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L

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G

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Q

I

I

E

E

D

D

H

H

L

L

G

G

D

D

G

G

S

S

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R

F

F

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G

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L

S

S

I

I

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R

Y

Y

S

S

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P

E

E

G

G

E

E

P

P

L

L

E

E

T

T

G

G

I

I

F

F

K

K

A

A

L

L

P

P

L

L

G

G

D

D

A

A

P

P

F

F

L

L

V

V

N
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N

G

G

D
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D

V

V

W

W

T

T

D

D

Y

Y

D

D

F

F

A

A

R

R

L

L

Q

Q

A

A

P

P

L

L

Q

Q

G

G

L

L

A

A

H

H

L

L

V

V

L

L

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V

D

D

N

N

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P

G

G

H

H

G

G

R

R

G

G

D
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D

F

F

R

R

L

L

V

V

G

G

E

E

Q

Q

V

V

D

D

G

G

D

D

A

A

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P

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G

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T

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T

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G

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H

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P

A

A

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F

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E

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C

C

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Q

A

A

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G

A

A

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F

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A

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R

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Q

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A

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M

A

A

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G

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E

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Y

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R

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H

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W

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V

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G

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T

L

L

E

E

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R

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L

A

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A

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E

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S

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L

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I

G

G

E

E

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R

A

A

GER 11:13 (= GER 11:13) binding
K23 (= K23) binding
N105 (= N105) binding
D107 (= D107) binding
D140 (= D140) binding
D205 (= D205) binding ; binding

4y7uA Structural analysis of muru (see paper)
100% identity, 100% coverage: 1:223/223 of query aligns to 1:223/224 of 4y7uA

query
sites
4y7uA

M

M

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K

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A

M

M

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I

L
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L

A
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A

G
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G

K

K

G
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G

E
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E

R
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R

M

M

R

R

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P

L

L

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T

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L

H

H

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T

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K
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K

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L

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A

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G

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Q

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P

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L

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I

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E

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Y

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R

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A

L

L

A

A

G

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T

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E

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I

N

N

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H

A

A

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W

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L

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G

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Q

I

I

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E

D

D

H

H

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L

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D

D

G

G

S

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F

F

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L

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S

I

I

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R

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Y

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S

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P

E

E

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G

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E

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L

E
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E

T

T

G

G

I

I

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F

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K

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A

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L

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P

L

L

G

G

D

D

A

A

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P

F

F

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L

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N
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G

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D

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V

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W

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T

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D

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Y

D

D

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F

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A

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A

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Q

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G

L

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D

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N

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P

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H

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D

F

F

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R

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L

V

V

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G

E

E

Q

Q

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V

D

D

G

G

D

D

A

A

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P

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G

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T

F
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F

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S

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G

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I

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S

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V

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L

H

H

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P

A

A

L

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F

F

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E

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C

C

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A

A

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G

A

A

F

F

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K

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L

A

A

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P

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L

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R

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Q

A

A

M

M

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A

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G

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K

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G

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G

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T

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L

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E

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R

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A

E

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A

A

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A

A

binding 5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]uridine: L6 (= L6), A7 (= A7), A8 (= A8), G9 (= G9), G11 (= G11), E12 (= E12), R13 (= R13), K23 (= K23), E84 (= E84), D107 (= D107)
binding 2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-1-O-phosphono-alpha-D-glucopyranose: N105 (= N105), D140 (= D140), F160 (= F160), W203 (= W203), D205 (= D205)
binding magnesium ion: D107 (= D107), D205 (= D205)

4y7vA Structural analysis of muru (see paper)
98% identity, 99% coverage: 1:221/223 of query aligns to 1:216/216 of 4y7vA

query
sites
4y7vA

M

M

K

K

A

A

M

M

I

I

L

L

A

A

G
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G

K
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K

G
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G

E
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E

R
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R

M

M

R

R

P

P

L

L

T

T

L

L

H

H

T

T

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K
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K

P

P

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L

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V

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P

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V

A

A

G

G

Q

Q

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P

L

L

I

I

E

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Y

Y

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R

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A

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L

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A

G

G

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V

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T

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E

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V

I

I

N

N

H

H

A

A

W

W

L

L

G

G

Q

Q

I

I

E

E

D

D

H

H

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L

G

G

D

D

G

G

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S

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R

F

F

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G

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L

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S

I

I

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R

Y

Y

S

S

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P

E

E

G

G

E

E

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L

L

E

E

T

T

G

G

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I

F

F

K

K

A

A

L

L

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P

L

L

G

G

D

D

A

A

P

P

F

F

L

L

V

V

N
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N

G

G

D

D

V

V

W

W

T

T

D

D

Y

Y

D

D

F

F

A

A

R

R

L

L

Q

Q

A

A

P

P

L

L

Q

Q

G

G

L

L

A

A

H

H

L

L

V

V

L

L

V

V

D

D

N

N

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P

G

-

H

-

G

G

R

R

G

G

D
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D

F

F

R

R

L

L

V

V

G

G

E

E

Q

Q

V

V

D

D

G

G

D

D

A

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P

-

G

G

T

T

L

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T

T

F
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F

S

S

G

G

I

I

S

S

V

V

L

L

H

H

P

P

A

A

L

L

F

F

E

E

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G

C

C

Q

Q

A

A

G

G

A

A

F

F

K

K

L

L

A

A

P

P

L

L

R

R

Q

Q

A

A

M

M

A

A

G

G

K

K

V

V

S

S

G

G

E

E

H

H

Y

Y

R

R

G

G

H

H

W
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W

V

V

D
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D

V

V

G

G

T

T

L

L

E

E

R

R

L

L

A

A

E

E

A

A

E

E

S

S

L

L

I

I

G

G

E

E

binding imidodiphosphoric acid: G9 (= G9), K10 (= K10), G11 (= G11), E12 (= E12), R13 (= R13), K23 (= K23)
binding 2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-1-O-phosphono-alpha-D-glucopyranose: N105 (= N105), D137 (= D140), F155 (= F160), W198 (= W203), D200 (= D205)

7d73E Cryo-em structure of gmppa/gmppb complex bound to gtp (state i) (see paper)
32% identity, 93% coverage: 1:207/223 of query aligns to 1:220/360 of 7d73E

query
sites
7d73E

M

M

K

K

A

A

M

L

I

I

L
|
L

A
x
V

A
x
G

G
|
G

K

Y

G
|
G

E
x
T

R
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R

M

L

R

R

P

P

L

L

T

T

L

L

H

S

T

T

P

P

K

K

P

P

L

L

V

V

P

D

V

F

A

C

G

N

Q

K

P

P

L

I

I

L

E

L

Y

H

H

Q

L

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R

E

A

A

L

L

A

A

G

G

V

V

T

D

E

H

V

V

V

I

I

L

N
x
A

H

V

A
x
S

W

Y

L

M

G

S

Q

Q

Q

V

I

L

E

E

D

K

H

E

L

M

-

K

G

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D

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G

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L

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G

L

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S

R

I

I

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M

S

S

P

H

E
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E

G

E

E

E

P
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P

L

L

E
x
G

T

T

G

A

G

G

G

P

I

L

F

A

K

L

A

A

L

R

P

D

L

L

G

S

D

E

A

T

-

A

-

D

P

P

F

F

L

F

L

V

V

L

N
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N

G

S

D

D

V

V

W

I

T

C

D

D

Y

F

D

P

F

F

A

-

R

-

L

-

Q

Q

A

A

P

M

L

V

Q

Q

G

F

L

H

A

R

H

H

-

H

-

G

-

Q

-

E

-

G

-

S

-

I

-

L

L

V

V

T

L

K

V

V

D

E

N

E

P

P

G

S

H

K

H

Y

G

G

-

V

-

C

R

E

G

A

D

D

F

T

R

G

L

R

V

I

G

H

E

R

Q

F

V

V

V

E

D

K

G

P

D

Q

D

V

A

F

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N

L

K

T

I

F

N

S

A

G

G

I

M

S

Y

V

I

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H

S

P

P

A

A

L

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F

L

E

Q

G

R

C

I

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Q

A

L

G

Q

A

P

F

T

K

S

L

I

A

E

P

K

L

E

L

V

R

F

Q

P

A

I

M

M

A

A

A

K

-

E

G

G

K

Q

V

L

S

Y

G

A

E

M

H

E

Y

L

R

Q

G

G

H

F

W

W

V

M

D

D

V

I

G

G

binding guanosine-5'-triphosphate: L6 (= L6), V7 (≠ A7), G8 (≠ A8), G9 (= G9), G11 (= G11), T12 (≠ E12), R13 (= R13), A52 (≠ N52), S54 (≠ A54), E80 (= E79), P83 (= P82), G85 (≠ E84), N108 (= N105)

7d72K Cryo-em structures of human gmppa/gmppb complex bound to gdp-mannose (see paper)
32% identity, 93% coverage: 1:207/223 of query aligns to 1:220/360 of 7d72K

query
sites
7d72K

M

M

K

K

A

A

M

L

I

I

L
|
L

A
x
V

A
x
G

G

G

K

Y

G

G

E

T

R

R

M

L

R

R

P

P

L

L

T

T

L

L

H

S

T

T

P

P

K

K

P

P

L

L

V

V

P

D

V

F

A

C

G

N

Q

K

P

P

L

I

I

L

E

L

Y

H

H

Q

L

V

R

E

A

A

L

L

A

A

G

G

V

V

T

D

E

H

V

V

V

I

I

L

N

A

H

V

A

S

W

Y

L

M

G

S

Q

Q

Q

V

I

L

E

E

D

K

H

E

L

M

-

K

G

A

D

Q

G

E

S

Q

R

R

F

L

G

G

L

I

S

R

I

I

R

S

Y

M

S

S

P

H

E

E

G

E

E

E

P

P

L

L

E
x
G

T
|
T

G

A

G

G

G

P

I

L

F

A

K

L

A

A

L

R

P

D

L

L

G

S

D

E

A

T

-

A

-

D

P

P

F

F

L

F

L

V

V

L

N
|
N

G
x
S

D
|
D

V

V

W

I

T

C

D

D

Y

F

D

P

F

F

A

-

R

-

L

-

Q

Q

A

A

P

M

L

V

Q

Q

G

F

L

H

A

R

H

H

-

H

-

G

-

Q

-

E

-

G

-

S

-

I

-

L

L

V

V

T

L

K

V

V

D

E

N

E

P

P

G

S

H

K

H

Y

G

G

-

V

-

C

R

E

G

A

D

D

F

T

R

G

L

R

V

I

G

H

E

R

Q

F

V

V

V

E

D

K

G

P

D

Q

D

V

A

F

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V

G

S

T

N

L

K

T

I

F
x
N

S

A

G

G

I

M

S

Y

V

I

L

L

H

S

P

P

A

A

L

V

F

L

E

Q

G

R

C

I

Q

Q

A

L

G

Q

A

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F

T

K

S

L

I

A
x
E

P

K

L

E

L

V

R

F

Q

P

A

I

M

M

A

A

A

K

-

E

G

G

K

Q

V

L

S

Y

G

A

E

M

H

E

Y

L

R

Q

G

G

H

F

W

W

V

M

D
|
D

V

I

G

G

binding guanosine-5'-diphosphate-alpha-d-mannose: L6 (= L6), V7 (≠ A7), G8 (≠ A8), G85 (≠ E84), T86 (= T85), N108 (= N105), S109 (≠ G106), D110 (= D107), N172 (≠ F160), E194 (≠ A182), D218 (= D205)

7d72E Cryo-em structures of human gmppa/gmppb complex bound to gdp-mannose (see paper)
32% identity, 93% coverage: 1:207/223 of query aligns to 1:220/360 of 7d72E

query
sites
7d72E

M

M

K

K

A

A

M

L

I

I

L
|
L

A
x
V

A
x
G

G

G

K

Y

G

G

E

T

R

R

M

L

R

R

P

P

L

L

T

T

L

L

H

S

T

T

P

P

K

K

P

P

L

L

V

V

P

D

V

F

A

C

G

N

Q

K

P

P

L

I

I

L

E

L

Y

H

H

Q

L

V

R

E

A

A

L

L

A

A

G

G

V

V

T

D

E

H

V

V

V

I

I

L

N

A

H

V

A
x
S

W

Y

L

M

G

S

Q

Q

Q

V

I

L

E

E

D

K

H

E

L

M

-

K

G

A

D

Q

G

E

S

Q

R

R

F

L

G

G

L

I

S

R

I

I

R

S

Y

M

S

S

P

H

E

E

G

E

E

E

P

P

L

L

E
x
G

T

T

G

A

G

G

G

P

I

L

F

A

K

L

A

A

L

R

P

D

L

L

G

S

D

E

A

T

-

A

-

D

P

P

F

F

L

F

L

V

V

L

N

N

G
x
S

D
|
D

V

V

W

I

T

C

D

D

Y

F

D

P

F

F

A

-

R

-

L

-

Q

Q

A

A

P

M

L

V

Q

Q

G

F

L

H

A

R

H

H

-

H

-

G

-

Q

-

E

-

G

-

S

-

I

-

L

L

V

V

T

L

K

V

V

D

E

N

E

P

P

G

S

H

K

H

Y

G

G

-

V

-

C

R

E

G

A

D

D

F

T

R

G

L

R

V

I

G

H

E

R

Q

F

V

V

V

E

D

K

G

P

D

Q

D

V

A

F

P

V

G

S

T

N

L

K

T

I

F
x
N

S

A

G

G

I

M

S

Y

V

I

L

L

H

S

P

P

A

A

L

V

F

L

E

Q

G

R

C

I

Q

Q

A

L

G

Q

A

P

F

T

K

S

L

I

A
x
E

P

K

L

E

L

V

R

F

Q

P

A

I

M

M

A

A

A

K

-

E

G

G

K

Q

V

L

S

Y

G

A

E

M

H

E

Y

L

R

Q

G

G

H

F

W

W

V

M

D
|
D

V

I

G

G

binding guanosine-5'-diphosphate-alpha-d-mannose: L6 (= L6), V7 (≠ A7), G8 (≠ A8), S54 (≠ A54), G85 (≠ E84), S109 (≠ G106), N172 (≠ F160), E194 (≠ A182), D218 (= D205)
binding magnesium ion: D110 (= D107), D218 (= D205)

7whsA Cryo-em structure of leishmanial gdp-mannose pyrophosphorylase in complex with gtp (see paper)
30% identity, 93% coverage: 1:207/223 of query aligns to 1:220/366 of 7whsA

query
sites
7whsA

M

M

K

R

A

A

M

V

I

I

L
|
L

A
x
V

A
x
G

G
|
G

K

F

G
|
G

E

T

R
|
R

M

L

R

R

P

P

L

L

T

T

L

L

H

T

T

T

P

P

K

K

P

P

L

L

V

V

P

P

V

F

A

C

G

N

Q

K

P

P

L

M

I

I

E

I

Y

H

H

Q

L

I

R

E

A

A

L

L

A

K

A

A

A

V

G

G

V

V

T

T

E

E

V

V

V

I

I

L

N
x
A

H

V

A

A

W

Y

L

R

G

P

Q

E

Q

A

I

M

E

K

D

E

H

Q

L

M

G

D

D

E

G

W

S

S

R

R

-

K

F

L

G

G

L

V

S

S

I

F

R

V

Y

F

S

S

P

V

E

E

G

E

E

E

P

P

L

L

E
x
G

T

T

G

A

G

G

G

P

I

L

F

A

K

L

A

A

L

R

P

D

L

I

L

L

-

M

-

Q

G

D

D

D

A

K

P

P

F

F

L

F

L

V

V

L

N
|
N

G

S

D
|
D

V

V

W

T

T

C

D

T

Y

F

-

P

-

M

-

Q

-

E

-

L

D

D

F

F

A

H

R

K

-

A

-

H

-

G

-

E

-

G

-

T

-

I

-

M

L

V

Q

S

A

Q

P

V

L

T

Q

Q

G

W

L

E

A

K

H

Y

L

G

V

V

L

V

V

V

D

Y

N

S

P

P

G

Q

H

N

H

Y

G

Q

R

I

G

E

D

R

F

F

-

V

-

E

-

K

-

P

-

S

R

R

L

F

V

L

G

G

E

D

Q

R

V

I

V

-

D

-

G

-

D

-

A

-

P

-

G

-

T

-

L

-

T

-

F

N

S

A

G

G

I

I

S

Y

V

I

L

F

H

N

P

K

A

S

L

I

F

L

E

D

G

R

C

I

Q

P

A

P

G

R

A

R

F

A

K

S

L

I

A

E

P

K

L

E

L

I

R

F

Q

P

A

A

M

M

A

A

-

E

G

G

K

Q

V

L

S

Y

G

A

E

F

H

N

Y

L

R

E

G

G

H

F

W

W

V

M

D
|
D

V

V

G

G

binding guanosine-5'-triphosphate: L6 (= L6), V7 (≠ A7), G8 (≠ A8), G9 (= G9), G11 (= G11), R13 (= R13), A52 (≠ N52), G85 (≠ E84), N108 (= N105), D110 (= D107)
binding magnesium ion: D110 (= D107), D218 (= D205)

P74285 UTP--glucose-1-phosphate uridylyltransferase; Cyanobacterial UDP-glucose pyrophosphorylase; UDP-glucose pyrophosphorylase; UDP-Glc PPase; EC 2.7.7.9 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
31% identity, 87% coverage: 1:193/223 of query aligns to 1:201/388 of P74285

query
sites
P74285

M

M

K

K

A

A

M

M

I

I

L

L

A

A

A
|
A

G

G

K

K

G

G

E

T

R

R

M

V

R

R

P

P

L

I

T

T

L

H

H

T

T

I

P

P

K

K

P

P

L

M

V

I

P

P

V

I

A

L

G

Q

Q

K

P

P

L

V

I

M

E

E

Y

F

H

L

L

L

R

E

A

L

L

L

A

R

A

Q

A

H

G

G

V

F

T

D

E

Q

V

I

V

M

I

V

N

N

H

V

A

S

W

H

L

L

G

A

Q

E

I

I

E

E

D

S

H

Y

L

F

G

R

D

D

G

G

S

Q

R

R

F

F

G

G

L

V

S

Q

I

I

R

A

Y

Y

S

S

P

F

E

E

-

G

-

N

-

I

-

V

-

D

-

G

-

D

-

L

-

V

G

G

E

K

P

A

L

L

E

G

T

S

G

A

G

G

I

L

F

K

K

K

A

I

L

Q

P

E

L

F

-

N

-

P

L

F

G

F

D

D

A

D

P

T

F

F

L

V

V

L

N

C

G

G

D

D

V

A

W

L

T

I

D

D

Y

L

D

D

F

L

A

T

-

T

-

A

-

V

R

K

L

L

Q

H

A

R

P

E

L

K

Q

G

G

A

L

I

A

A

H

T

L

I

V

I

L

T

V

K

D

T

N

V

P

P

G

Q

H

E

H

-

G

-

R

-

G

-

D

-

F

-

R

-

L

L

V

V

G

S

E

S

Q

Y

-

G

V

V

D

V

G

T

D

D

A

N

P

G

G

K

T

I

L

L

T

T

F

F

S

Q

G

E

I

K

S

P

V

A

L

V

H

E

P

E

A

A

L

L

F

S

E

T

G

E

C

I

Q

N

A

T

G

G

A

I

F

Y

K

I

L

F

A

E

P

P

L

E

L

V

R

I

Q

D

A

Y

M

I

A

P

A

S

G

G

K

Q

A8 (= A8) mutation to G: Two-fold decrease in affinity for UTP. No effect on affinity for Glc-1P and on catalytic activity rate.

7whtA Cryo-em structure of leishmanial gdp-mannose pyrophosphorylase in complex with gdp-mannose (see paper)
42% identity, 52% coverage: 1:117/223 of query aligns to 1:120/360 of 7whtA

query
sites
7whtA

M

M

K

R

A

A

M

V

I

I

L
|
L

A
x
V

A

G

G
|
G

K

F

G
|
G

E

T

R

R

M

L

R

R

P

P

L

L

T

T

L

L

H

T

T

T

P

P

K
|
K

P

P

L

L

V

V

P

P

V

F

A

C

G

N

Q

K

P

P

L

M

I

I

E

I

Y

H

H

Q

L

I

R

E

A

A

L

L

A

K

A

A

A

V

G

G

V

V

T

T

E

E

V

V

V

I

I

L

N

A

H

V

A

A

W

Y

L

R

G

P

Q

E

Q

A

I

M

E

K

D

E

H

Q

L

M

G

D

D

E

G

W

S

S

R

R

-

K

F

L

G

G

L

V

S

S

I

F

R

V

Y

F

S

S

P

V

E

E

G

E

E

E

P
|
P

L
|
L

E

G

T

T

G

A

G

G

G

P

I

L

F

A

K

L

A

A

L

R

P

D

L

I

L

L

-

M

-

Q

G

D

D

D

A

K

P

P

F

F

L

F

L

V

V

L

N
|
N

G
x
S

D
|
D

V

V

W

T

T

C

D

T

Y

F

D

P

F

M

A

Q

R

E

L

L

binding guanosine-5'-diphosphate-alpha-d-mannose: L6 (= L6), V7 (≠ A7), G9 (= G9), G11 (= G11), K23 (= K23), P83 (= P82), L84 (= L83), N108 (= N105), S109 (≠ G106), D110 (= D107)
binding magnesium ion: D110 (= D107)

Sites not aligning to the query:

7x8kA Arabidopsis gdp-d-mannose pyrophosphorylase (vtc1) structure (product- bound) (see paper)
31% identity, 93% coverage: 1:207/223 of query aligns to 1:220/365 of 7x8kA

query
sites
7x8kA

M

M

K

K

A

A

M

L

I

I

L

L

A
x
V

A
x
G

G

G

K

F

G

G

E

T

R

R

M

L

R

R

P

P

L

L

T

T

L

L

H

S

T

F

P

P

K

K

P

P

L

L

V

V

P

D

V

F

A

A

G

N

Q

K

P

P

L

M

I

I

E

L

Y

H

H

Q

L

I

R

E

A

A

L

L

A

K

A

A

A

V

G

G

V

V

T

D

E

E

V

V

I

L

N

A

H

I

A

N

W

Y

L

Q

G

P

Q

E

Q

V

I

M

E

L

D

N

H

F

L

L

G

K

D

D

-

F

G

E

S

T

R

K

F

L

G

E

L

I

S

K

I

I

R

T

Y

C

S

S

P

Q

E
|
E

G

T

E

E

P

P

L

L

E
x
G

T

T

G

A

G

G

G
x
P

I

L

F

A

K

L

A

A

L

R

P

D

L

K

L

L

G

L

D

D

A

G

-

S

-

G

-

E

P

P

F

F

L

F

L

V

V

L

N
|
N

G

S

D
|
D

V

V

W

I

T

S

D

E

Y

Y

-

P

-

L

-

K

D

E

F

M

A

L

R

E

L

F

Q

H

A

K

P

S

L

H

Q

G

G

G

L

E

A

A

H

S

L

I

V

M

L

V

-

T

-

K

V

V

D

D

N

E

P

P

G

S

H

K

H

Y

G
|
G

-

V

-

M

-

E

R

E

G

S

D

T

F

G

R

R

L

V

V

-

G

-

E

E

Q

K

V

F

V

V

D
x
E

G

K

D

P

D

L

A

Y

P

V

G

G

T

N

L

K

T

I

F
x
N

S

A

G
|
G

I

I

S

Y

V

L

L

L

H

N

P

P

A

S

L

V

F

L

E

D

G

K

C

I

Q

E

A

L

G

R

A

P

F

T

K

S

L

I

A
x
E

P

K

L

E

L

T

R

F

Q

P

A

K

M

I

A

A

G

A

K

Q

-

G

V

L

S

Y

G

A

E

M

H

V

Y

L

R

P

G

G

H

F

W

W

V

M

D

D

V

I

G

G

binding guanosine-5'-diphosphate-alpha-d-mannose: V7 (≠ A7), G8 (≠ A8), E80 (= E79), G85 (≠ E84), P89 (≠ G88), N109 (= N105), D111 (= D107), G146 (= G137), E162 (≠ D150), N172 (≠ F160), G174 (= G162), E194 (≠ A182)

O22287 Mannose-1-phosphate guanylyltransferase 1; GDP-mannose pyrophosphorylase 1; Protein CYTOKINESIS DEFECTIVE 1; Protein EMBRYO DEFECTIVE 101; Protein HYPERSENSITIVE TO AMMONIUM ION 1; Protein SENSITIVE TO OZONE 1; Protein VITAMIN C DEFECTIVE 1; EC 2.7.7.13 from Arabidopsis thaliana (Mouse-ear cress) (see 5 papers)
30% identity, 93% coverage: 1:207/223 of query aligns to 1:221/361 of O22287

query
sites
O22287

M

M

K

K

A

A

M

L

I

I

L
|
L

A
x
V

A

G

G
|
G

K

F

G
|
G

E
x
T

R
|
R

M

L

R

R

P

P

L

L

T

T

L

L

H

S

T

F

P
|
P

K
|
K

P

P

L

L

V

V

P
x
D

V

F

A

A

G

N

Q

K

P

P

L

M

I

I

E

L

Y

H

H

Q

L

I

R

E

A

A

L

L

A

K

A

A

A

V

G

G

V

V

T

D

E

E

V

V

I

L

N

A

H

I

A

N

W

Y

L

Q

G

P

Q

E

Q

V

I

M

E

L

D

N

H

F

L

L

G

K

D

D

-

F

G

E

S

T

R

K

F

L

G

E

L

I

S

K

I

I

R

T

Y

C

S

S

P

Q

E

E

G

T

E

E

P

P

L

L

E
x
G

T

T

G

A

G

G

G
x
P

I

L

F

A

K

L

A

A

L

R

P

D

L

K

L

L

G

L

D

D

A

G

-

S

-

G

-

E

P

P

F

F

L

F

L

V

V

L

N
|
N

G

S

D
|
D

V

V

W

I

T

S

D

E

Y

Y

-

P

-

L

-

K

D

E

F

M

A

L

R

E

L

F

Q

H

A

K

P

S

L

H

Q

G

G

G

L

E

A

A

H

S

L

I

V

M

L

V

-

T

-

K

V

V

D

D

N

E

P

P

G

S

H

K

H

Y

G
|
G

R

V

G

V

D

V

F

M

R

E

L

E

V

S

G

T

E

G

Q

R

V

V

V

E

D

K

G

F

D

V

D

E

A

K

P

P

-

K

-

L

-

Y

-

V

G

G

T

N

L

K

T

I

F
x
N

S

A

G

G

I

I

S

Y

V

L

L

L

H

N

P

P

A

S

L

V

F

L

E

D

G

K

C

I

Q

E

A

L

G

R

A

P

F

T

K

S

L

I

A

E

P

K

L

E

L

T

R

F

Q

P

A

K

M

I

A

A

G

A

K

Q

-

G

V

L

S

Y

G

A

E

M

H

V

Y

L

R

P

G

G

H

F

W

W

V

M

D

D

V

I

G

G

L6 (= L6) binding
V7 (≠ A7) binding
G9 (= G9) binding
G11 (= G11) binding ; mutation to S: In hsn1; reduced enzyme activity, ascorbate concentrations and N-glycosylation, and increased sensitivity to ammonium.
T12 (≠ E12) binding
R13 (= R13) binding
P22 (= P22) mutation to S: In vtc1-1 and vtc1-2; reduced enzyme activity and ascorbate concentrations, and ozone-sensitive.
K23 (= K23) binding
D27 (≠ P27) mutation to E: Abolishes interaction with CSN5B and subsequent degradation in the dark by the 26S proteasome, and increases ascorbate accumulation in seedlings.
G85 (≠ E84) binding
P89 (≠ G88) mutation to L: In cyt1-1; deficient in N-glycosylation and cellulose, and embryo lethal.
N109 (= N105) binding
D111 (= D107) binding
G146 (= G137) binding
N173 (≠ F160) binding

Sites not aligning to the query:

223:361 mutation Missing: Reduces catalytic activity 3-fold.

7x8kB Arabidopsis gdp-d-mannose pyrophosphorylase (vtc1) structure (product- bound) (see paper)
30% identity, 93% coverage: 1:207/223 of query aligns to 1:221/367 of 7x8kB

query
sites
7x8kB

M

M

K

K

A

A

M

L

I

I

L

L

A

V

A

G

G
|
G

K
x
F

G
|
G

E
x
T

R

R

M

L

R

R

P

P

L

L

T

T

L

L

H

S

T

F

P

P

K
|
K

P

P

L

L

V

V

P

D

V

F

A

A

G

N

Q

K

P

P

L

M

I

I

E

L

Y

H

H

Q

L

I

R

E

A

A

L

L

A

K

A

A

A

V

G

G

V

V

T

D

E

E

V

V

I

L

N

A

H

I

A

N

W

Y

L

Q

G

P

Q

E

Q

V

I

M

E

L

D

N

H

F

L

L

G

K

D

D

-

F

G

E

S

T

R

K

F

L

G

E

L

I

S

K

I

I

R

T

Y

C

S

S

P

Q

E

E

G

T

E

E

P

P

L

L

E

G

T

T

G

A

G

G

G

P

I

L

F

A

K

L

A

A

L

R

P

D

L

K

L

L

G

L

D

D

A

G

-

S

-

G

-

E

P

P

F

F

L

F

L

V

V

L

N

N

G

S

D

D

V

V

W

I

T

S

D

E

Y

Y

-

P

-

L

-

K

D

E

F

M

A

L

R

E

L

F

Q

H

A

K

P

S

L

H

Q

G

G

G

L

E

A

A

H

S

L

I

V

M

L

V

-

T

-

K

V

V

D

D

N

E

P

P

G

S

H

K

H

Y

G

G

R

V

G

V

D

V

F

M

R

E

L

E

V

S

G

T

E

G

Q

R

V

V

V

E

D

K

G

F

D

V

D

E

A

K

P

P

-

K

-

L

-

Y

-

V

G

G

T

N

L

K

T

I

F

N

S

A

G

G

I

I

S

Y

V

L

L

L

H

N

P

P

A

S

L

V

F

L

E

D

G

K

C

I

Q

E

A

L

G

R

A

P

F

T

K

S

L

I

A

E

P

K

L

E

L

T

R

F

Q

P

A

K

M

I

A

A

G

A

K

Q

-

G

V

L

S

Y

G

A

E

M

H

V

Y

L

R

P

G

G

H

F

W

W

V

M

D

D

V

I

G

G

binding pyrophosphate: G9 (= G9), F10 (≠ K10), G11 (= G11), T12 (≠ E12), K23 (= K23)

7d73B Cryo-em structure of gmppa/gmppb complex bound to gtp (state i) (see paper)
27% identity, 93% coverage: 1:208/223 of query aligns to 2:236/406 of 7d73B

query
sites
7d73B

M

L

K

K

A

A

M

V

I

I

L
|
L

A
x
I

A

G

G

G

-

P

-

Q

K

K

G

G

E

T

R

R

M

F

R

R

P

P

L

L

T

S

L

F

H

E

T

V

P

P

K

K

P

P

L

L

V

F

P

P

V

V

A

A

G

G

Q

V

P

P

L

M

I

I

E

Q

Y

H

H

H

L

I

R

E

A

A

L

C

A

A

A

Q

A

V

-

P

G

G

V

M

T

Q

E

E

V

I

V

L

I

L

N
x
I

H

G

A
x
F

W

Y

L

Q

G

P

Q

D

Q

E

I

P

E

L

D

T

H

Q

L

F

G

L

D

E

G

A

S

A

R

Q

-

Q

-

E

F

F

G

N

L

L

S

P

I

V

R

R

Y

Y

S

L

P

Q

E
|
E

G

F

E

A

P

P

L

L

E
x
G

T

T

G

G

I

L

F

Y

K

H

A

F

L

R

-

D

P

Q

L

I

L

L

G

A

D

G

A

S

P

P

-

E

-

A

F

F

L

F

L

V

V

L

N

N

G

A

D
|
D

V

V

W

C

T

S

D

D

Y

F

D

P

F

L

A

S

-

A

-

M

-

L

-

E

-

A

-

H

-

R

R

R

L

Q

Q

R

A

H

P

P

L

F

-

L

-

G

-

T

-

A

-

N

-

R

-

T

Q

Q

G

S

L

L

A

N

H

Y

L

G

V

C

L

I

V

V

D

E

N

N

P

P

G

Q

H

T

H

H

G

-

R

-

G

-

D

-

F

-

R

-

L

-

V

-

G

-

E

-

Q

E

V

V

V

L

D

H

G

Y

D

V

D
x
E

A

K

P

P

G

S

T

T

-

F

-

I

-

S

-

D

L

I

T

I

F

N

S

C

G

G

I

I

S

Y

V

L

L

F

H

S

P

P

A

E

-

A

-

L

-

K

-

P

-

L

-

R

-

D

L

V

F

F

E

Q

G

R

C

N

Q

Q

A

Q

G

G

A

T

F

I

K

R

L

L

A
x
E

P

Q

L

D

L

V

R

F

Q

S

A

A

M

L

A

A

-

G

A

Q

G

G

K

Q

V

I

S

Y

G

V

E

H

H

L

Y

T

R

D

G

G

H

I

W

W

V

S

D

Q

V

I

G

K

T

S

binding guanosine-5'-triphosphate: L7 (= L6), I8 (≠ A7), I56 (≠ N52), F58 (≠ A54), E85 (= E79), G90 (≠ E84), D116 (= D107), E169 (≠ D153), E209 (≠ A182)

7d72A Cryo-em structures of human gmppa/gmppb complex bound to gdp-mannose (see paper)
32% identity, 61% coverage: 1:136/223 of query aligns to 2:162/407 of 7d72A

query
sites
7d72A

M

L

K

K

A

A

M

V

I

I

L
|
L

A

I

A
x
G

G

G

-

P

-

Q

K
|
K

G

G

E

T

R

R

M

F

R

R

P

P

L

L

T

S

L

F

H

E

T

V

P

P

K

K

P

P

L

L

V

F

P

P

V

V

A

A

G

G

Q

V

P

P

L

M

I

I

E

Q

Y

H

H

H

L

I

R

E

A

A

L

C

A

A

A

Q

A

V

-

P

G

G

V

M

T

Q

E

E

V

I

V

L

I

L

N

I

H

G

A
x
F

W

Y

L

Q

G

P

Q

D

Q

E

I

P

E

L

D

T

H

Q

L

F

G

L

D

E

G

A

S

A

R

Q

-

Q

-

E

F

F

G

N

L

L

S

P

I

V

R

R

Y

Y

S

L

P

Q

E
|
E

G

F

E

A

P

P

L

L

E
x
G

T

T

G

G

I

L

F

Y

K

H

A

F

L

R

-

D

P

Q

L

I

L

L

G

A

D

G

A

S

P

P

-

E

-

A

F

F

L

F

L

V

V

L

N
|
N

G

A

D

D

V

V

W

C

T

S

D

D

Y

F

D

P

F

L

A

S

-

A

-

M

-

L

-

E

-

A

-

H

-

R

R

R

L

Q

Q

R

A

H

P

P

L

F

-

L

-

G

-

T

-

A

-

N

-

R

-

T

Q

Q

G

S

L

L

A

N

H

Y

L

G

V

C

L

I

V

V

D

E

N

N

P

P

G

Q

H

T

H

H

binding guanosine-5'-diphosphate-alpha-d-mannose: L7 (= L6), G9 (≠ A8), K13 (= K10), F58 (≠ A54), E85 (= E79), G90 (≠ E84), N114 (= N105)

Sites not aligning to the query:

binding guanosine-5'-diphosphate-alpha-d-mannose: 170, 180, 210, 234, 236

1lvwA Crystal structure of glucose-1-phosphate thymidylyltransferase, rmla, complex with dtdp
29% identity, 98% coverage: 1:219/223 of query aligns to 4:240/295 of 1lvwA

query
sites
1lvwA

M

M

K

K

A

G

M

I

I

V

L
|
L

A

A

A
x
G

G

G

K

S

G

G

E

T

R

R

M

L

R

Y

P

P

L

I

T

T

L

R

H

A

T

V

P

S

K

K

P
x
Q

L

L

V

L

P

P

V

I

A

Y

G

D

Q

K

P

P

L

M

I

I

E

Y

Y

Y

H

P

L

L

R

S

A

V

L

L

A

M

A

L

A

A

G

G

V

I

T

R

E

D

V

I

V

L

I

I

N

I

H

S

A

T

W

P

L

R

G

D

Q

L

Q

P

I

L

-

Y

E

R

D

D

H

L

L

L

G

G

D

D

G

G

S

S

R

Q

F

F

G

G

L

V

S

R

I

F

R

S

Y

Y

S

R

P

V

E
x
Q

G

E

E

E

P

P

L

R

E
x
G

T

I

G

A

G

D

G

A

I

F

F

I

K

V

A

G

L

K

P

D

L

F

L

I

G

G

D

D

A

S

P

K

F

V

L

A

L

L

V

V

N
x
L

G

G

D
|
D

-

N

V

V

W

F

T
x
Y

D
x
G

Y
x
H

D
x
R

F
|
F

A
x
S

-

E

-

I

-

L

R

R

L

R

Q

A

A

A

P

S

L

L

Q

E

G

D

L

G

A

A

H

V

L

I

V

F

-

G

-

Y

L

Y

V

V

D

R

N

D

P

P

G

R

H

P

H

F

G

G

R

V

G

V

D

E

F

F

R

D

L

S

V

E

G

G

E

-

Q

R

V

V

V

I

D

S

G

I

D

E

A

K

P

P

G

S

T

R

-

P

-

K

-

S

-

N

-

Y

-

V

-

P

-

G

L

L

T

Y

F

F

S

Y

G

D

I

N

S

Q

V

V

L

V

H

E

P

I

A

A

-

R

L

R

F

I

E

E

G

P

C

S

Q

D

A

R

G

G

A

E

F

L

K

E

L

I

A

T

P

S

L

V

L

N

R

E

Q

E

A

Y

M

L

A

R

A

M

G

G

K

K

V

L

S

R

G

V

E

E

H

L

Y

M

-

G

R

R

G

G

H

M

-

A

W

W

V

L

D

D

V

T

G

G

T

T

L

H

E

D

R

G

L

L

A

L

E

E

A

A

E

S

S

S

L

F

I

I

binding thymidine-5'-diphosphate: L9 (= L6), G11 (≠ A8), Q27 (≠ P24), Q83 (≠ E79), G88 (≠ E84), L109 (≠ N105), D111 (= D107), Y115 (≠ T110), G116 (≠ D111), H117 (≠ Y112), R118 (≠ D113), F119 (= F114), S120 (≠ A115)

Sites not aligning to the query:

binding thymidine-5'-diphosphate: 251, 257

6jq8A Crystal structure of hddc from yersinia pseudotuberculosis complexed with gmp-pn (see paper)
26% identity, 62% coverage: 3:140/223 of query aligns to 4:144/225 of 6jq8A

query
sites
6jq8A

A

V

M

V

I

I

L
|
L

A
|
A

A
x
G

G
|
G

K

L

G

G

E

T

R

R

M

L

R

K

P

S

L

V

T

S

L

G

H

D

T

I

P

P

K

K

P

P

L

M

V

V

P

D

V

I

A

S

G

G

Q

K

P

P

L

F

I

L

E

Y

Y

R

H

L

L

M

R

E

A

Y

L

L

A

E

A

D

A

Q

G

G

V

V

T

Q

E

R

V

I

I

L

N
x
S

H

L

A

S

W

Y

L

K

G

A

Q

D

Q

Y

I

I

E

I

D

D

H

S

L

V

G

I

D

K

G

D

S

N

R

P

F

V

G

N

L

S

S

K

I

V

R

D

Y

F

S

I

P

I

E
|
E

G

N

E

E

P
|
P

L

L

E
x
G

T

T

G

G

G
x
A

I

I

F

K

K

N

A

A

L

C

P

K

L

Y

L

I

G

E

D

A

A

S

P

K

F

F

L

I

L

V

V

I

N
|
N

G

G

D
|
D

V

T

W

Y

T

C

D

E

Y

L

D

D

F

Y

A

K

R

K

-

F

L

I

Q

E

A

S

P

S

L

L

Q

D

G

S

L

D

A

L

H

Q

L

I

-

S

-

G

V

V

L

E

V

V

D

D

N

D

P

V

G

C

H

R

H

Y

G

G

R

S

G

L

D

D

binding aminophosphonic acid-guanylate ester: L7 (= L6), A8 (= A7), G9 (≠ A8), G10 (= G9), S53 (≠ N52), E80 (= E79), P83 (= P82), G85 (≠ E84), A89 (≠ G88), N106 (= N105), D108 (= D107)

5ifyA Crystal structure of glucose-1-phosphate thymidylyltransferase from burkholderia vietnamiensis in complex with 2 -deoxyuridine-5'- monophosphate and 2'-deoxy-thymidine-b-l-rhamnose
26% identity, 98% coverage: 2:220/223 of query aligns to 3:239/293 of 5ifyA

query
sites
5ifyA

K

K

A

G

M

I

I

I

L
|
L

A

A

A
x
G

G

G

K

S

G

G

E

T

R

R

M

L

R

Y

P

P

L

I

T

T

L

H

H

A

T

V

P

S

K

K

P

Q

L

L

V

L

P

P

V

V

A

Y

G

D

Q

K

P

P

L

M

I

I

E

Y

Y

Y

H

P

L

L

R

S

A

T

L

L

A

M

A

I

A

A

G

G

V

I

T

R

E

D

V

V

-

L

V

I

I

I

N

S

H

T

A

P

W

Q

L

D

G

T

Q

P

Q

R

I

F

E

E

D

S

H

M

L

L

G

G

D

D

G

G

S

S

R

Q

F

W

G

G

L

M

S

N

I

I

R

Q

Y

Y

S

A

P

T

E
x
Q

G

P

E

S

P
|
P

L

D

E
x
G

T
x
L

G

A

G

Q

G

A

I

F

F

V

K

I

A

G

L

R

P

D

L

F

L

V

G

G

D

N

A

E

P

P

F

S

L

A

L

L

V

I

N

L

G

G

D
|
D

-

N

V

I

W

F

T
x
Y

D
x
G

Y
x
H

D
|
D

F

L

A

A

R

K

L

Q

-

L

-

E

Q

R

A

A

P

S

L

A

Q

K

G

D

L

T

A

G

H

A

L

T

V

V

L

F

-

A

-

Y

-

H

V

V

D

Q

N

D

P

P

G

E

H

R

H
x
Y

G
|
G

R

V

G

V

D

E

F

F

R

D

L

R

V

E

G

F

E

R

Q

A

V

I

V

S

D

I

G

E

D
x
E

D
x
K

A

P

P

A

G

K

T

P

L

R

T

S

F

S

S

Y

G

A

I
x
V

S

T

V

G

L

L

H
x
Y

P

F

A

Y

L

D

F

R

E

Q

G

V

C

C

Q

D

A

I

-

A

-

D

-

I

-

K

-

P

-

S

-

A

-
x
R

G

G

A

E

F

L

K

E

L

I

A

T

P

D

L

V

L

N

R

S

Q

R

A

Y

M

L

A

A

G

G

K

Q

V

L

S

D

G

V

E

E

H

L

Y

M

-
x
G

R
|
R

G
|
G

H

Y

-

A

W

W

V

L

D

D

V

T

G

G

T

T

L

H

E

D

R

S

L

L

A

I

E

E

A

A

E

G

S

T

L

F

I

I

G

A

binding 2'-deoxy-thymidine-beta-l-rhamnose: L7 (= L6), G9 (≠ A8), Q81 (≠ E79), P84 (= P82), G86 (≠ E84), L87 (≠ T85), D109 (= D107), Y144 (≠ H136), G145 (= G137), E160 (≠ D152), K161 (≠ D153), V171 (≠ I163), Y175 (≠ H167), R193 (vs. gap)
binding 2'-deoxyuridine 5'-monophosphate: Y113 (≠ T110), G114 (≠ D111), H115 (≠ Y112), D116 (= D113), G217 (vs. gap), R218 (= R200), G219 (= G201)

Sites not aligning to the query:

binding 2'-deoxyuridine 5'-monophosphate: 254, 255

P26393 Glucose-1-phosphate thymidylyltransferase; dTDP-glucose pyrophosphorylase; Ep; dTDP-glucose synthase; EC 2.7.7.24 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
27% identity, 98% coverage: 2:220/223 of query aligns to 5:241/292 of P26393

query
sites
P26393

K

K

A

G

M

I

I

I

L

L

A

A

A

G

G

G

K

S

G

G

E

T

R

R

M

L

R

Y

P

P

L

V

T

T

L

M

H

A

T

V

P

S

K

K

P

Q

L

L

V

L

P

P

V

I

A

Y

G

D

Q

K

P

P

L

M

I

I

E

Y

Y

Y

H

P

L

L

R

S

A

T

L

L

A

M

A

L

A

A

G

G

V

I

T

R

E

D

V

I

-

L

V

I

I

I

N

S

H

T

A

P

W

Q

L

D

G

T

Q

P

Q

R

I

F

E

Q

D

Q

H

L

L

L

G

G

D

D

G

G

S

S

R

Q

F

W

G

G

L

L

S

N

I

L

R

Q

Y

Y

S

K

P

V

E

Q

G

P

E

S

P

P

L

D

E

G

T

L

G

A

G

Q

G

A

I

F

F

I

K

I

A

G

L

E

P

E

L

F

L

I

G

G

D

H

A

D

P

D

F

C

L

A

L

L

V

V

N

L

G

G

D

D

-

N

V

I

W

F

T

Y

D

G

Y

H

D

D

F

L

A

P

R

K

L

L

-

M

Q

E

A

A

P

A

L

V

Q

N

G

K

L

E

A

S

H

G

L

A

V

T

L

V

-

F

-

A

-

Y

-

H

V

V

D

N

N

D

P

P

G

E

H

R

H

Y

G

G

R

V

G

V

D

E

F

F

R

D

L

Q

V

K

G

G

E

T

Q

A

V

V

V

S

-

L

-

E

-

K

-

P

-

L

-

Q

-

P

D

K

G

S

D

N

D

Y

A

A

P

V

G

T

T

G

L

L

T

Y

F

F

S

Y

G

D

I

N

S

S

V

V

L

V

H

E

P

M

A

A

L

K

-

N

F

L

E

K

G

P

C

S

Q

A

A

R

G

G

A

E

F

L

K

E

L

I

A
x
T

P

D

L

I

L

N

R

R

Q

I

A

Y

M

M

A

E

A

Q

G

G

K

R

V

L

S

S

G

V

E

A

H

M

Y

M

-

G

R

R

G

G

H

Y

-

A

W
|
W

V

L

D

D

V

T

G

G

T

T

L

H

E

Q

R

S

L

L

A

I

E

E

A

A

E

S

S

N

L

F

I

I

G

A

T201 (≠ A182) mutation to A: Two-fold increase in the conversion of 2-acetamido-2-deoxy-alpha-D-glucopyranosyl phosphate.
W224 (= W203) mutation to H: Is able to convert both 6-acetamido-6-deoxy-alpha-D-glucopyranosyl phosphate and alpha-D-glucopyranuronic acid 1-(dihydrogen phosphate), which are not accepted by the wild-type.

3pkpA Q83s variant of s. Enterica rmla with datp (see paper)
27% identity, 98% coverage: 2:220/223 of query aligns to 5:241/290 of 3pkpA

query
sites
3pkpA

K

K

A

G

M

I

I

I

L

L

A

A

A
x
G

G
|
G

K
x
S

G
|
G

E
x
T

R
|
R

M

L

R

Y

P

P

L

V

T

T

L

M

H

A

T

V

P

S

K
|
K

P
x
Q

L

L

V

L

P

P

V

I

A

Y

G

D

Q

K

P

P

L

M

I

I

E

Y

Y

Y

H

P

L

L

R

S

A

T

L

L

A

M

A

L

A

A

G

G

V

I

T

R

E

D

V

I

-

L

V

I

I

I

N

S

H

T

A

P

W

Q

L

D

G

T

Q

P

Q

R

I

F

E

Q

D

Q

H

L

L

L

G

G

D

D

G

G

S

S

R

Q

F

W

G

G

L

L

S

N

I

L

R

Q

Y

Y

S

K

P

V

E
x
S

G

P

E

S

P
|
P

L

D

E

G

T

L

G

A

G

Q

G

A

I

F

F

I

K

I

A

G

L

E

P

E

L

F

L

I

G

G

D

H

A

D

P

D

F

C

L

A

L

L

V

V

N

L

G
|
G

D
|
D

-

N

V

I

W

F

T

Y

D

G

Y

H

D

D

F

L

A

P

R

K

L

L

-

M

Q

E

A

A

P

A

L

V

Q

N

G

K

L

E

A

S

H

G

L

A

V

T

L

V

-

F

-

A

-

Y

-

H

V

V

D

N

N

D

P

P

G

E

H

R

H

Y

G

G

R

V

G

V

D

E

F

F

R

D

L

Q

V

K

G

G

E

T

Q

A

V

V

V

S

-

L

-

E

-

K

-

P

-

L

-

Q

-

P

D

K

G

S

D

N

D

Y

A

A

P

V

G

T

T

G

L

L

T

Y

F

F

S

Y

G

D

I

N

S

S

V

V

L

V

H

E

P

M

A

A

L

K

-

N

F

L

E

K

G

P

C

S

Q

A

A

R

G

G

A

E

F

L

K

E

L

I

A

T

P

D

L

I

L

N

R

R

Q

I

A

Y

M

M

A

E

A

Q

G

G

K

R

V

L

S

S

G

V

E

A

H

M

Y

M

-

G

R

R

G

G

H

Y

-

A

W

W

V

L

D

D

V

T

G

G

T

T

L

H

E

Q

R

S

L

L

A

I

E

E

A

A

E

S

S

N

L

F

I

I

G

A

binding 2'-deoxyadenosine 5'-triphosphate: G11 (≠ A8), G12 (= G9), S13 (≠ K10), G14 (= G11), T15 (≠ E12), R16 (= R13), K26 (= K23), Q27 (≠ P24), S83 (≠ E79), P86 (= P82), G110 (= G106), D111 (= D107)

3pkpB Q83s variant of s. Enterica rmla with datp (see paper)
27% identity, 98% coverage: 2:220/223 of query aligns to 4:240/289 of 3pkpB

query
sites
3pkpB

K

K

A

G

M

I

I

I

L

L

A

A

A
x
G

G
|
G

K
x
S

G
|
G

E
x
T

R
|
R

M

L

R

Y

P

P

L

V

T

T

L

M

H

A

T

V

P

S

K
|
K

P
x
Q

L

L

V

L

P

P

V

I

A

Y

G

D

Q

K

P

P

L

M

I

I

E

Y

Y

Y

H

P

L

L

R

S

A

T

L

L

A

M

A

L

A

A

G

G

V

I

T

R

E

D

V

I

-

L

V

I

I

I

N

S

H

T

A

P

W

Q

L

D

G

T

Q

P

Q

R

I

F

E

Q

D

Q

H

L

L

L

G

G

D

D

G

G

S

S

R

Q

F

W

G

G

L

L

S

N

I

L

R

Q

Y

Y

S

K

P

V

E
x
S

G

P

E

S

P
|
P

L

D

E

G

T

L

G

A

G

Q

G

A

I

F

F

I

K

I

A

G

L

E

P

E

L

F

L

I

G

G

D

H

A

D

P

D

F

C

L

A

L

L

V

V

N

L

G
|
G

D
|
D

-

N

V

I

W

F

T

Y

D

G

Y

H

D

D

F

L

A

P

R

K

L

L

-

M

Q

E

A

A

P

A

L

V

Q

N

G

K

L

E

A

S

H

G

L

A

V

T

L

V

-

F

-

A

-

Y

-

H

V

V

D

N

N

D

P

P

G

E

H

R

H

Y

G

G

R

V

G

V

D

E

F

F

R

D

L

Q

V

K

G

G

E

T

Q

A

V

V

V

S

-

L

-

E

-

K

-

P

-

L

-

Q

-

P

D

K

G

S

D

N

D

Y

A

A

P

V

G

T

T

G

L

L

T

Y

F

F

S

Y

G

D

I

N

S

S

V

V

L

V

H

E

P

M

A

A

L

K

-

N

F

L

E

K

G

P

C

S

Q

A

A

R

G

G

A

E

F

L

K

E

L

I

A

T

P

D

L

I

L

N

R

R

Q

I

A

Y

M

M

A

E

A

Q

G

G

K

R

V

L

S

S

G

V

E

A

H

M

Y

M

-

G

R

R

G

G

H

Y

-

A

W

W

V

L

D
|
D

V

T

G

G

T

T

L

H

E

Q

R

S

L

L

A

I

E

E

A

A

E

S

S

N

L

F

I

I

G

A

binding 2'-deoxyadenosine 5'-triphosphate: G10 (≠ A8), G11 (= G9), S12 (≠ K10), G13 (= G11), T14 (≠ E12), R15 (= R13), K25 (= K23), Q26 (≠ P24), S82 (≠ E79), P85 (= P82), G109 (= G106), D110 (= D107)
binding magnesium ion: D110 (= D107), D225 (= D205)

Query Sequence

>PP_0406 FitnessBrowser__Putida:PP_0406
MKAMILAAGKGERMRPLTLHTPKPLVPVAGQPLIEYHLRALAAAGVTEVVINHAWLGQQI
EDHLGDGSRFGLSIRYSPEGEPLETGGGIFKALPLLGDAPFLLVNGDVWTDYDFARLQAP
LQGLAHLVLVDNPGHHGRGDFRLVGEQVVDGDDAPGTLTFSGISVLHPALFEGCQAGAFK
LAPLLRQAMAAGKVSGEHYRGHWVDVGTLERLAEAESLIGERA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory