SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PP_0414 FitnessBrowser__Putida:PP_0414 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
29% identity, 70% coverage: 71:262/275 of query aligns to 85:285/296 of P68183

query
sites
P68183

L

L

K

W

N

N

S

S

I

V

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V

A

A

P

G

A

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A

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E190 (≠ R171) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A173) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G177) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P190) mutation to A: No effect.

P94530 Arabinooligosaccharides transport system permease protein AraQ from Bacillus subtilis (strain 168) (see paper)
25% identity, 49% coverage: 61:194/275 of query aligns to 67:198/281 of P94530

query
sites
P94530

F

F

F

N

G

G

S

G

A

S

E

I

W

Y

M

F

R

K

A

W

L

F

K

F

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N

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D180 (≠ L176) mutation to A: Negative impact in the growth rate in the presence of arabinotriose.

Query Sequence

>PP_0414 FitnessBrowser__Putida:PP_0414
MLSPYMSPVERVWFYSLRILCGLILLFLILPVLVIVPLSFNSGSFLVYPLQGFSLQWYQD
FFGSAEWMRALKNSIIVAPAATVLAMVFGTLAAIGLTRGDFPGKSLVMALVISPMVVPVV
IIGVASYLFFAPLGMGNSFTSLILVHAVLGVPFVIITVSATLQGFNYNLVRAAASLGASP
LLAFRRVTLPLIAPGVISGALFAFATSFDEVVVTLFLAGPEQATLPRQMFSGIRENLSPT
IAAAATLLIAFSVLLLLTLEWLRGRSEKLRTQQPA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory