SitesBLAST

Y102 (= Y105) mutation to L: Retains 4% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
W106 (= W109) mutation to L: Retains 20% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
K163 (= K166) mutation to A: Retains 24% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
F216 (= F220) mutation to L: Retains 13% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
E222 (= E226) mutation to A: Abolishes lysine uptake. Strongly inhibits CadC.
E230 (= E234) mutation to V: Abolishes lysine uptake. Shows significant less inhibition of CadC.
D275 (vs. gap) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-278.
D278 (vs. gap) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-275.
E438 (≠ D434) mutation to A: Retains 14% of wild-type lysine uptake activity. Is unable to inhibit CadC.
D443 (vs. gap) mutation to A: Retains 11% of wild-type lysine uptake activity. Is unable to inhibit CadC.
D446 (vs. gap) mutation to A: Retains 13% of wild-type lysine uptake activity. Is unable to inhibit CadC.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P46349 Gamma-aminobutyric acid permease; GABA permease; 4-aminobutyrate permease; Gamma-aminobutyrate permease; Proline transporter GabP from Bacillus subtilis (strain 168) (see paper)
39% identity, 90% coverage: 10:441/479 of query aligns to 2:429/469 of P46349

query
sites
P46349

N

N

A

Q

S

S

A

Q

H

S

T

G

F

L

K

K

Q

K

D

E

M

L

Q

K

T

T

R

R

H

H

I

M

V

T

M

M

L

I

A

S

L

I

G

A

G

G

V

V

I

I

G

G

T

A

G

G

L

L

F

F

L

V

T
x
G

S

S

G

G

Y

S

T

V

V

I

N

H

Q

S

A

T

G
|
G

P

P

L

-

G

G

A

A

V

V

I

V

A

S

Y

Y

I

A

L

L

G

A

A

G

I

L

M

L

V

V

Y

I

L

F

V

I

M

M

M

R

C

M

L

L

G

G

E

E

L

M

A

S

V

A

H

V

M

N

P

P

E

T

V

S

G

G

S

S

F

F

S

S

S

Q

Y

Y

A

A

T

H

R

D

Y

A

L

I

G

G

P

P

G

W

T

A

G

G

Y

F

M

T

V

I

A

G

W

W

M

L

Y

Y

W

W

L

F

T

F

W

W

T

V

V

I

A

V

I

I

G

A

S

I

E

E

F

A

T

I

A

A

A

G

G

A

I

G

L

I

M

I

V

Q

R

Y

W

W

F

F

P

H

D

D

T

I

P

P

V

L

W

W

M

L

W

T

S

S

A

L

L

I

F

L

G

T

A

I

A

V

V

L

F

T

I

L

S

T

N

N

I

V

I

Y

S

S

V

V

R

K

S

S

F

F

A

G

E

E

T

F

E

E

F

Y

W

W

L

F

S

S

L

L

V

I

K

K

V

V

L

V

A

T

V

I

I

I

A

A

F

F

L

L

V

I

V

V

G

G

G

F

G

A

A

F

I

I

L

F

G

G

G

-

F

-

E

F

I

A

Q

P

Q

G

A

S

H

E

S

P

A

V

G

G

L

F

G

S

N

N

F

L

T

T

-

G

R

K

E

G

G

G

L

F

F

F

P

P

T

E

G

G

F

I

W

S

S

S

I

V

A

L

M

L

T

G

L

I

L

V

A

V

V

V

A

I

F

F

A

S

F

F

S

M

G

G

T

T

E

E

L

I

I

V

G

A

I

I

A

A

G

G

E

E

T

T

K

S

D

N

P

P

E

I

K

E

N

S

V

V

P

T

K

K

A

A

I

T

R

R

T

S

T

V

V

V

L

W

R

R

L

I

A

I

L

V

F

F

F

Y

I

V

G

G

T

S

I

I

F

A

V

I

L

V

A

V

T

A

L

L

P

P

R

W

E

N

Q

S

A

A

G

N

V

I

I

L

E

E

S

S

P

P

F

F

V

V

M

A

V

V

F

L

A

E

M

H

I

I

G

G

I

V

P

P

Y

A

A

A

D

Q

I

I

M

M

N

N

F

F

V

I

I

V

I

L

T

T

A

A

L

V

L

L

S

S

A

C

A

L

N

N

S

S

G
|
G

L

L

Y

Y

A

T

S

S

R

R

M

M

L

L

W

Y

T

S

L

L

S

A

D

E

Q

R

G

N

N

E

M

A

P

P

R

R

Y

F

A

M

T

K

L

L

S

S

R

K

G

G

T

V

P

P

F

V

N

Q

A

A

I

I

V

V

L

A

S
x
G

M

T

A

F

G
x
F

G

S

M

Y

A

I

S

A

L

V

S

M

S

N

V

Y

F

F

A

S

P

P

D

D

T

T

I

V

Y

F

L

L

A

F

L

L

V

V

S

N

I

S

S

S

G

G

L

A

A

I

V

A

V

L

V

V

W

Y

I

L

S

V

I

I

A

A

A

V

S

S

Q

Q

I

L

A

K

F

M

R

R

R

K

H

K

Y

L

I

E

A

K

S

T

G

N

G

P

K

-

L

-

E

E

D

A

L

L

K

K

F

I

R

K

V

M

R

W

G

L

Y

F

P

P

F

F

V

L

P

T

L

Y

G

L

A

T

I

I

F

I

C

A

C

I

V

C

L
x
G

A

I

C

L

V

V

G

S

I

M

A

A

F

F

D

I

P

D

A

S

Q

M

R

R

V

D

A

E

L

L

G33 (≠ T41) mutation to D: Lack of activity.
G42 (= G50) mutation to S: Lack of activity.
G301 (= G311) mutation to V: Lack of activity.
G338 (≠ S348) mutation to E: Lack of activity.
F341 (≠ G351) mutation to S: Lack of activity.
G414 (≠ L426) mutation to R: Lack of activity.

P24207 Phenylalanine-specific permease; Phenylalanine:H(+) symporter PheP from Escherichia coli (strain K12) (see 3 papers)
38% identity, 86% coverage: 2:415/479 of query aligns to 5:412/458 of P24207

query
sites
P24207

S

S

V

T

Q

V

T

S

T

E

T

D

T

T

R

A

N

S

A

N

S

Q

A

E

H

P

T

T

F

L

K

H

Q

R

D

G

M

L

Q

H

T

N

R
|
R

H

H

I

I

V

Q

M

L

L

I

A

A

L

L

G

G

V

A

I

I

G

G

T

T

G

G

L

L

F

F

L

L

T

G

S

I

G

G

Y

P

T

A

V

I

N

Q

Q

M

A

A

G

G

P
|
P

L

-

G

A

A

V

V

L

I

L

A

G

Y

Y

I

G

L

V

G

A

A

G

I

I

M

I

V

A

Y

F

L

L

V

I

M

M

M

R

C

Q

L

L

G

G

E

E

L

M

A

V

V

V

H

E

M

E

P

P

E

V

V

S

G

G

S

S

F
|
F

S

A

S

H

Y
x
F

A

A

T
x
Y

R

K

Y
|
Y

L
x
W

G

G

P

P

G
x
F

T

A

G

G

Y
x
F

M

L

V

S

A

G

W
|
W

M

N

Y
|
Y

W
|
W

L

V

T

M

W
x
F

T

V

V

L

A

V

I

G

G

M

S

A

E
|
E

F

L

T

T

A

A

G

G

I

I

L

Y

M

M

V

Q

R

Y

W

W

F

F

P

P

D

D

T

V

P

P

V

T

W

W

M

I

W

W

S

A

A

A

L

A

F

F

G

F

A

I

V

I

F

N

I

A

S

V

N

N

I

L

I

V

S

N

V

V

R

R

S

L

F

Y

A

G

E

E

T

T

E

E

F

F

W

W

L

F

S

A

L

L

V

I

K
|
K

V

V

L

L

A

A

V

I

I

I

A

G

F

M

L

I

V

G

V

F

G

G

G

L

G

W

A

L

I

L

L

F

G

S

G

G

F

-

E

-

I

-

Q

H

Q

G

A

G

H

E

S

K

A

A

G

S

L

I

G

D

N

N

F

L

T

W

R

R

E

Y

G

G

-

G

L

F

F

F

P

A

T

T

G

G

F

W

W

N

S

G

I

L

A

I

M

L

T

S

L

L

L

A

A

V

V

I

A

M

F

F

A

S

F

F

S

G

G

G

T

L

E
|
E

L

L

I

I

G

G

I

I

A

T

A

A

G

A

E

E

T

A

K

R

D

D

P

P

E

E

K

K

N

S

V

I

P

P

K

K

A

A

I

V

R

N

T

Q

T

V

V

V

L

Y

R
|
R

L

I

A

L

L

L

F

F

F

Y

I

I

G

G

T

S

I

L

F

V

V

V

L

L

A

L

T

A

L

L

L

Y

P

P

R

W

E

V

Q

E

A

V

G

K

V

S

I

N

E

S

S

S

P

P

F

F

V

V

M

M

V

I

F

F

A

H

M

N

I

L

G

D

I

S

P

N

Y

V

A

V

A

A

D

S

I

A

M

L

N

N

F

F

V

V

I

I

I

L

T

V

A

A

L

S

L

L

S

S

A

V

A

Y

N

N

S

S

G

G

L

V

Y

Y

A

S

A

N

S

S

R

R

M

M

L

L

W

F

T

G

L

L

S

S

D

V

Q

Q

G

G

N

N

M

A

P

P

R

K

R

F

Y

L

A

T

T

R

L

V

S

S

R

R

G

G

T

V

P
|
P

F

I

N

N

A

S

I

L

V

M

L

L

S

S

M

G

A

A

G

I

G

T

M

S

A

L

S

V

L

V

L

L

S

I

S

N

V

Y

F

L

A

L

P

P

D

Q

T

K

I

A

Y

F

L

G

A

L

L

L

V

M

S

A

I

L

S

V

G

V

L

A

A

T

V

L

V

N

W

W

I

I

S

M

I

I

A

C

A

L

S

A

Q

H

I

L

A

R

F

F

R

R

R

A

H

A

Y

M

I

R

A

R

S

Q

G

G

G

-

K

-

L

-

E

R

D

E

L

T

K

Q

F

F

R

K

V

A

R

L

G

L

Y

Y

P

P

F

F

R26 (= R23) mutation R->G,S,Q: Strong decrease in phenylalanine transport activity.
P54 (= P51) mutation to A: 50% of wild-type phenylalanine transport activity.; mutation to G: No change in phenylalanine transport activity.; mutation to L: 26% of wild-type phenylalanine transport activity.
F87 (= F85) mutation to L: No effect on phenylalanine transport activity.
F90 (≠ Y88) mutation to L: 65% of wild-type phenylalanine transport activity.
Y92 (≠ T90) mutation to L: 41% of wild-type phenylalanine transport activity.
Y94 (= Y92) mutation to L: 69% of wild-type phenylalanine transport activity.
W95 (≠ L93) mutation to L: 10% of wild-type phenylalanine transport activity.
F98 (≠ G96) mutation to L: No effect on phenylalanine transport activity.
F101 (≠ Y99) mutation to L: 38% of wild-type phenylalanine transport activity.
W105 (= W103) mutation to L: 39% of wild-type phenylalanine transport activity.
Y107 (= Y105) mutation to L: No effect on phenylalanine transport activity.
W108 (= W106) mutation to L: 71% of wild-type phenylalanine transport activity.
F111 (≠ W109) mutation to L: 60% of wild-type phenylalanine transport activity.; mutation to Y: Enables the transport of tryptophan to almost the same steady-state level as that of phenylalanine.
E118 (= E116) mutation E->G,L,V,N: Loss of activity.
K168 (= K166) mutation K->L,R: Strong decrease in phenylalanine transport activity.; mutation to N: Loss of activity.
E226 (= E226) mutation E->A,Q,K,R,W: Loss of activity.
R252 (= R252) mutation R->D,E,F,W,P: Loss of activity.
P341 (= P341) mutation to A: 5% of wild-type phenylalanine transport activity.; mutation P->G,Q,K,R: Loss of activity.; mutation to S: 3% of wild-type phenylalanine transport activity.; mutation to T: 17% of wild-type phenylalanine transport activity.

Sites not aligning to the query:

442 P→A: 46% of wild-type phenylalanine transport activity.; P→G: 52% of wild-type phenylalanine transport activity.; P→L: 43% of wild-type phenylalanine transport activity.

P15993 Aromatic amino acid transport protein AroP; Aromatic amino acid:H(+) symporter AroP; General aromatic amino acid permease; General aromatic transport system from Escherichia coli (strain K12) (see paper)
36% identity, 80% coverage: 14:394/479 of query aligns to 9:386/457 of P15993

query
sites
P15993

H

E

T

Q

F

L

K

K

Q

R

D

G

M

L

Q

K

T

N

R

R

H

H

I

I

V

Q

M

L

L

I

A

A

L

L

G

G

V

A

I

I

G

G

T

T

G

G

L

L

F

F

L

L

T

G

S

S

G

A

Y

S

T

V

V

I

N

Q

Q

S

A

A

G

G

P

P

L

-

G

G

A

I

V

I

I

L

A

G

Y

Y

I

A

L

I

G

A

A

G

I

F

M

I

V

A

Y

F

L

L

V

I

M

M

M

R

C

Q

L

L

G

G

E

E

L

M

A

V

V

V

H

E

M

E

P

P

E

V

V

A

G

G

S

S

F

F

S

S

S

H

Y

F

A

A

T

Y

R

K

Y

Y

L

W

G

G

P

S

G

F

T

A

G

G

Y

F

M

A

V

S

A

G

W

W

M

N

Y

Y

W

W

L

V

T

L

W
x
Y

T

V

V

L

A

V

I

A

G

M

S

A

E

E

F

L

T

T

A

A

A

V

G

G

I

K

L

Y

M

I

V

Q

R

F

W

W

F

Y

P

P

D

E

T

I

P

P

V

T

W

W

M

V

W

S

S

A

A

A

L

V

F

F

G

F

A

V

V

I

F

N

I

A

S

I

N

N

I

L

I

T

S

N

V

V

R

K

S

V

F

F

A

G

E

E

T

M

E

E

F

F

W

W

L

F

S

A

L

I

V

I

K

K

V

V

L

I

A

A

V

V

I

V

A

A

F

M

L

I

V

I

V

F

G

G

G

W

A

L

I

L

L

F

G

S

G

G

F

N

E

G

I

G

Q

P

Q

Q

A

A

H

T

S

V

A

S

G

N

L

L

G

-

N

-

F

W

T

D

R

Q

E

G

G

G

L

F

F

L

P

P

T

H

G

G

F

F

W

T

S

G

I

L

A

V

M

M

T

M

L

M

L

A

A

I

V

I

A

M

F

F

A

S

F

F

S

G

G

G

T

L

E

E

L

L

I

V

G

G

I

I

A

T

A

A

G

A

E

E

T

A

K

D

D

N

P

P

E

E

K

Q

N

S

V

I

P

P

K

K

A

A

I

T

R

N

T

Q

T

V

V

I

L

Y

R

R

L

I

A

L

L

I

F

F

F

Y

I

I

G

G

T

S

I

L

F

A

V

V

L

L

A

L

T

S

L

L

L

M

P

P

R

W

E

T

Q

R

A

V

G

T

V

A

I

D

E

T

S

S

P

P

F

F

V

V

M

L

V

I

F

F

A

H

M

E

I

L

G

G

I

D

P

T

Y

F

A

V

A

A

D

N

I

A

M

L

N

N

F

I

V

V

I

V

I

L

T

T

A

A

L

A

L

L

S

S

A

V

A

Y

N

N

S

S

G

C

L

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Y

Y

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C

A

N

S

S

R

R

M

M

L

L

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G

L

L

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A

D

Q

Q

Q

G

G

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N

M

A

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R

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Y

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A

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L

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F

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R

Y103 (≠ W109) Key residue for tryptophan transport; mutation to F: Decreases tryptophan transport to less than 50% of wild-type levels and reduces the ability of tryptophan to inhibit phenylalanine transport from 95 to 62%.

P04817 Arginine permease CAN1; Canavanine resistance protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
31% identity, 94% coverage: 17:464/479 of query aligns to 85:551/590 of P04817

query
sites
P04817

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-

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P113 (≠ T45) mutation to L: In CAN1-343; confers citrulline transport activity in GAP1-deleted cells.
P148 (= P80) mutation to L: In CAN1-337; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, but not sensitivity to L-aspartic acid alpha-hydroxamate or p-fluoro-L-phenylalanine.
V149 (≠ E81) mutation to F: In CAN1-315; confers citrulline transport activity in GAP1-deleted cells.
S152 (= S84) mutation to F: In CAN1-342; confers citrulline transport activity in GAP1-deleted cells.
Y173 (= Y105) mutation to D: In CAN1-306; confers citrulline transport activity in GAP1-deleted cells.; mutation to H: In CAN1-327; confers citrulline transport activity in GAP1-deleted cells.
G308 (= G233) mutation to A: In CAN1-341; confers citrulline transport activity in GAP1-deleted cells.
P313 (= P238) mutation to S: In CAN1-329; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, L-aspartic acid alpha-hydroxamate and p-fluoro-L-phenylalanine.
TS 354:355 (vs. gap) mutation Missing: In CAN1-318; confers citrulline transport activity in GAP1-deleted cells.
Y356 (vs. gap) mutation to H: In CAN1-340; confers citrulline transport activity in GAP1-deleted cells.; mutation to N: In CAN1-339; confers citrulline transport activity in GAP1-deleted cells.
W451 (≠ A369) mutation to C: In CAN1-328; confers citrulline transport activity in GAP1-deleted cells.; mutation to L: In CAN1-316; confers citrulline transport activity in GAP1-deleted cells.; mutation to S: In CAN1-335; confers citrulline transport activity in GAP1-deleted cells.
F461 (≠ V379) mutation to S: In CAN1-307; confers citrulline transport activity in GAP1-deleted cells.

Q9URZ4 Cationic amino acid transporter 1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
31% identity, 82% coverage: 17:408/479 of query aligns to 81:481/587 of Q9URZ4

query
sites
Q9URZ4

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F

Sites not aligning to the query:

29 modified: Phosphoserine
30 modified: Phosphoserine
37 modified: Phosphoserine

P19145 General amino-acid permease GAP1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
32% identity, 82% coverage: 17:409/479 of query aligns to 87:491/602 of P19145

query
sites
P19145

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x
A

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L

F

F

F

Y

I

I

G

L

T

S

I

L

F

L

V

M

L

I

A

G

T

L

L

L

L

V

P

P

R

Y

E

N

Q

D

A

K

G

S

V

L

I

I

-

G

-

A

-

S

-

V

-

D

-

A

E

A

S

S

P

P

F

F

V

V

M

I

V

A

F

I

A

K

M

T

I

H

G

G

I

I

P

K

Y

G

A

L

A

P

D

S

I

V

M

V

N

N

F

V

V

V

I

I

I

L

T

I

A

A

L

V

L

L

S

S

A

V

A

G

N

N

S

S

G

A

L

I

Y

Y

A

A

A

C

S

S

R

R

M

T

L

M

W

V

T

A

L

L

S

A

D

E

Q

Q

G

R

N

F

M

L

P

P

R

E

R

I

Y

F

A

S

T

Y

L

V

S

D

R

R

R

K

G

G

T

R

P

P

F

L

N

V

A

G

I

I

V

A

L

V

S

T

M

S

A

A

G

F

G

G

M

L

A

I

S

A

L

F

L

V

S

A

V

S

F

K

A

K

P

E

D

G

T

E

I

V

Y

F

L

N

A

W

L

L

V

L

S

A

I

L

S

S

G

G

L

L

A

S

V

S

V

L

V

F

V

T

W

W

I

G

S

G

I

I

A

C

A

I

S

C

Q

H

I

I

A

R

F

F

R

R

R

K

H

A

Y

L

I

A

A

A

S

Q

G

G

G

R

K

G

L

L

E

D

D

E

L

L

K

S

F

F

R

K

A297 (≠ S223) mutation to V: Impairs basic amino-acids transport and regulation by these amino-acids.

Sites not aligning to the query:

76 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

P48813 High-affinity glutamine permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
28% identity, 85% coverage: 4:409/479 of query aligns to 133:550/663 of P48813

query
sites
P48813

Q

Q

T

S

T

P

T

Q

T

E

R

Q

N

E

A

Q

S

K

A

Q

H

E

T

N

F

L

K

K

Q

K

D

S

M

I

Q

K

T

P

R

R

H

H

I

T

V

V

M

M

L

M

A

S

L

L

G

G

G

T

V

G

I

I

G

G

T

T

G

G

L

L

F

L

L

V

T

G

S

N

G

S

Y

K

T

V

V

L

N

N

Q

N

A

A

G

G

P

P

L

G

G

G

A

L

V

I

I

I

A

G

Y

Y

I

A

L

I

G

M

A

G

I

S

M

C

V

V

Y

Y

L

C

V

I

M

I

M

Q

C

A

L

C

G

G

E

E

L

L

A

A

V

V

H

I

M

Y

P

S

E

D

-

L

V

I

G

G

S

G

F

F

S

N

S

T

Y

Y

A

P

T

L

R

F

Y

L

L

V

G

D

P

P

G

A

T

L

G

G

Y

F

M

S

V

V

A

A

W

W

M

L

Y

F

W

C

L

L

T

Q

W

W

T

L

V

C

A

V

I

C

G

P

S

L

E

E

F

L

T

V

A

T

A

A

G

S

I

M

L

T

M

I

V

K

R

Y

W

W

F

T

P

T

D

S

T

V

P

N

V

P

W

D

M

V

W

F

S

V

A

V

L

I

F

F

G

Y

A

V

A

L

V

I

F

V

I

V

S

I

N

N

I

V

I

F

S

G

V

A

R

K

S

G

F

Y

A

A

E

E

T

A

E

D

F

F

W

F

L

F

S

N

L

C

V

C

K

K

V

I

L

L

A

M

V

I

I

V

A

G

F

F

L

F

V

I

V

L

G

A

-

I

-

D

-

C

G

G

A

A

I

G

L

T

G

D

G

G

F

Y

E

-

I

I

Q

G

Q

S

A

K

H

Y

S

W

A

R

G

D

L

P

G

G

N

A

F

F

T

-

R

-

E

R

G

G

L

D

F

T

P

P

-

I

T

Q

G

R

F

F

W

K

S

G

I

V

A

V

M

A

T

T

L

F

L

V

A

T

V

A

A

A

F

F

A

A

F

F

S

G

G

M

T

S

E

E

L

Q

I

L

G

A

I

M

A

T

A

A

G

S

E

E

T

Q

K

S

D

N

P

P

E

R

K

K

N

A

V

I

P

P

K

S

A

A

I

A

R

K

T

K

T

M

V

I

L

Y

R

R

L

I

A

L

L

F

F

V

F

F

I

L

G

A

T

S

I

L

F

T

V

L

L

V

A

G

T

F

L

L

L

V

P

P

R

Y

E

T

Q

S

A

D

G

Q

V

L

I

L

-

G

-

A

-

G

-

S

-

A

-

T

-

K

E

A

S

S

P

P

F

Y

V

V

M

I

V

A

F

V

A

S

M

S

I

H

G

G

I

V

P

R

Y

V

A

V

A

P

D

H

I

F

M

I

N

N

F

A

V

V

I

I

I

L

T

L

A

S

L

V

L

L

S

S

A

V

A

A

N

N

S

G

G

A

L

F

Y

Y

A

T

A

S

S

S

R

R

M

I

L

L

W

M

T

S

L

L

S

A

D

K

Q

Q

G

G

N

N

M

A

P

P

R

K

R

C

Y

F

A

D

T

Y

L

I

S

D

R

R

R

E

G

G

T

R

P

P

F

A

N

A

A

A

I

M

V

L

L

V

S

S

M

A

A

L

G

F

G

G

M

V

A

I

S

A

L

F

L

C

S

A

S

S

V

S

F

K

A

K

P

E

D

E

T

D

I

V

Y

F

L

T

A

W

L

L

V

L

S

A

I

I

S

S

G

G

L

L

A

S

V

Q

V

L

V

F

V

T

W

W

I

I

S

T

I

I

A

C

A

L

S

S

Q

H

I

I

A

R

F

F

R

R

H

A

Y

M

I

K

A

V

S

Q

G

G

G

R

K

S

L

L

E

G

D

E

L

V

K

G

F

Y

R

K

Sites not aligning to the query:

132 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q03770 SPS-sensor component SSY1; Amino-acid permease homolog SSY1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
22% identity, 87% coverage: 8:425/479 of query aligns to 269:761/852 of Q03770

query
sites
Q03770

T

S

R

R

N

A

A

T

S

R

A

K

H

Y

T

H

F

I

K

Q

Q

R

D

K

M

L

Q

K

T

V

R

R

H

H

I

I

V

Q

M

M

L

L

A

S

L

I

G

G

G

A

V

C

I

F

G

S

T

V

G

G

L

L

F

F

L

L

T

T

S

S

G

G

Y

K

T

A

V

F

N

S

Q

I

A

A

G

G

P

P

L

F

G

G

A

T

V

L

I

L

A

G

Y

F

I

G

L

L

G

T

A

G

I

S

M

I

V

I

Y

L

L

A

V

T

M

M

M

L

C

S

L

F

G

T

E

E

L

L

A

S

V

T

H

L

M

I

P

P

E

V

V

S

G

S

S

G

F

F

S

S

S

G

Y

L

A

A

T

S

R

R

Y

F

L

V

G

E

P

D

G

A

T

F

G

G

Y

F

M

A

V

L

A

G

W

W

M

T

Y

Y

W

W

L

I

T

S

W

C

T

M

V

L

A

A

I

L

G

P

S

A

E

Q

F

V

T

S

A

S

G

-

I
x
T

L

F

M

Y

V

L

R

S

W

Y

F

Y

P

N

D

N

T

V

P

N

V

I

W

S

M

K

-

G

-

V

-

T

-

A

-

G

W

F

S

I

A

T

L

L

F

F

G

S

A

A

A

F

V

S

F

I

I

V

S

V

N

N

I

L

S

D

V

V

R

S

S

I

F

M

A

G

E

E

T

I

E

V

F

Y

W

V

L

A

S

G

L

I

V

S

K

K

V

V

L

-

A

-

V

I

I

I

A

A

F

I

L

L

V

M

V

V

G

F

G

T

G

M

A

I

I

I

L

L

G

N

G

A

F

G

E

H

I

G

Q

N

Q

D

A

I

H

H

S

E

-

G

-

V

-

G

-

F

-

R

-

Y

-

W

-

D

-

S

-

K

-

S

-

V

-

R

-

N

-

L

-

T

-

Y

-

G

-

L

-

Y

-

R

-

P

-

T

-

F

-

D

-

L

A

A

G

D

L

A

G

G

N

E

F

G

T

S

R

K

E

K

G

G

L

I

F

S

-

G

P

P

T

K

G

G

-

R

F

F

W

L

S

A

I

T

A

A

M

S

T

V

L

M

L

L

A

I

V

S

A

T

F

F

A

A

F

F

S

S

G

G

T

V

E

E

L

M

I

T

G

F

I

L

A

A

A

S

G

G

E

E

T

A

K

I

D

N

P

P

E

R

K

K

N

T

V

I

P

P

K

S

A

A

I

T

R

K

T

R

T

T

V

F

L

S

R

I

L

V

A

L

L

I

F

S

F

Y

I

V

G

F

T

L

I

I

F

F

V

S

-

V

-

G

-

I

-

N

-

I

-

Y

-

S

-

G

-

D

-

P

-

R

L

L

A

L

T

S

L

Y

L

F

P

P

-

G

-

I

-

S

-

E

R

K

E

R

Q

Y

A

E

G

A

V

I

I

I

E

K

-

G

-

T

-

G

-

M

-

D

-

W

-

R

-

L

-

R

-

T

-

N

-

C

-

R

-

G

-

I

-

D

-

Y

-

R

-

Q

-

I

-

S

-

V

-

G

-

T

-

G

-

Y

-

S

S

S

P

P

F

W

V

V

M

V

V

A

F

L

A

Q

M

N

I

F

G

G

I

L

P

C

Y

T

A

F

A

A

D

S

I

A

M

F

N

N

F

A

V

I

I

L

I

I

T

F

A

F

L

T

L

A

S

T

A

A

A

G

N

I

S

S

G

S

L

L

Y

F

A

S

A

C

S

S

R

R

M

T

L

L

W

Y

T

A

L

M

S

S

D

V

Q

Q

G

R

N

K

M

A

P

P

R

P

R

V

Y

F

A

E

T

I

L

C

S

S

R

K

R

R

G

G

T

V

P

P

F

Y

N

V

A

S

I

V

V

I

L

F

S

S

M

S

A

L

G

F

G

S

M

V

A

I

S

A

L

Y

L

I

S

A

S

V

V

D

F

Q

A

T

P

A

D

I

T

E

I

N

Y

F

L

D

A

V

L

L

V

A

S

N

I

V

S

S

G

S

L

A

A

S

V

T

V

S

V

I

W

W

I

M

S

G

I

L

A

-

S

-

Q

N

I

L

A

S

F

F

R

L

R

R

H

F

Y

Y

I

Y

A

A

S

L

G

K

G

Q

K

R

L

K

E

D

D

I

L

I

K

S

F

R

R

N

V

D

R

S

G

S

Y

Y

-

P

-

Y

-

K

-

S

P

P

F

F

V

Q

P

P

L

Y

G

L

A

A

I

I

F

Y

C

G

C

L

V

V

T382 (≠ I122) mutation T->H,L: Constitutively active, up-regulates amino acid permease transcription in response to subthreshold concentrations of amino acids.; mutation to K: In SSY1-102; constitutively active, up-regulates amino acid permease transcription in the absence of amino-acids.; mutation to R: Constitutively active, up-regulates amino acid permease transcription in the absence of amino acids.

P76037 Putrescine importer PuuP from Escherichia coli (strain K12) (see paper)
27% identity, 53% coverage: 17:269/479 of query aligns to 19:265/461 of P76037

query
sites
P76037

K

R

Q

K

D

S

M

L

Q

K

T

L

R

W

H

Q

I

V

V

V

M

M

L

M

A

G

L

L

G

A

G

Y

V

L

I

T

G

P

T

M

G

T

L

V

F

F

L

D

T

T

S

F

G

G

Y

I

T

V

V

S

N

G

Q

I

A

S

G

D

P

-

L

-

G

G

A

H

V

V

I

P

A

A

-

S

Y

Y

I

L

L

L

G

A

A

L

I

A

M

G

V

V

Y

L

L

F

V

T

M

A

M

I

C

S

L

Y

G

G

E

K

L

L

A

V

V

R

H

Q

M

F

P

P

E

E

V

A

G

G

S

S

F

A

S

Y

S

T

Y

Y

A

A

T

Q

R

K

Y

S

L

I

G

N

P

P

G

H

T

V

G

G

Y

F

M

M

V

V

A

G

W

W

M

S

Y

S

W

L

L

L

T

D

W
x
Y

T

L

V

F

A

L

I

P

G

M

S

I

E

N

F

V

T

L

A

L

A

A

G

K

I

I

L

Y

M

L

V

S

R

A

W

L

F

F

P

P

D

E

T

V

P

P

V

P

W

W

M

V

W

W

S

V

A

V

L

T

F

F

G

V

A

A

A

I

V

L

F

T

I

A

S

A

N

N

I

L

I

K

S

S

V

V

R

N

S

L

F

V

A

A

-

N

-

F

E

N

T

T

E

L

F

F

W

V

L

L

S

V

L

Q

V

I

K

S

V

I

L

M

A

V

V

V

I

F

A

I

F

F

L

L

V

V

G

Q

G

G

G

-

A

-

I

-

L

-

G

-

F

-

E

-

I

L

Q

H

Q

K

A

G

H

E

S

G

A

V

G

G

-

T

-

V

-

W

-

S

L

L

G

Q

N

P

F

F

T

I

R

S

E

E

G

N

-

A

-

H

L

L

F

I

P

P

T

-

G

-

F

-

W

-

S

-

I

I

A

I

M

T

T

G

L

A

L

T

A

I

V

V

A

C

F

F

A

S

F

F

S

L

G

G

T

F

E

D

L

A

I

V

G

T

I

T

A

L

A

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G

E

E

E

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D

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N

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A

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Y110 (≠ W109) mutation to X: The uptake activity is reduced to one-eighth of that of wild-type.

6f34A Crystal structure of a bacterial cationic amino acid transporter (cat) homologue bound to arginine. (see paper)
23% identity, 86% coverage: 11:424/479 of query aligns to 18:421/458 of 6f34A

query
sites
6f34A

A

A

S

K

A

G

H

A

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S

F

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R

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K

D

E

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L

Q
x
G

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x
A

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x
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x
D

I

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M
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M

L

L

A

G

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I

G

G

G
x
A

V
x
I

I

I

G
|
G

T
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T

G
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G

L

I

F

F

L

V

T

L

S

T

G

G

Y

V

T

A

V

A

-

A

N

E

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H

A

A

G

G

P

P

L

-

G

A

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F

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I

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A

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W

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Y

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W

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x
E

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-

W

W

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A

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x
A

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x
R

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I
x
A

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x
V

Q

I

A

V

H
x
A

-
x
I

-

K

-

V

-
x
A

-

V

-
x
L

-

F

-

L

S
x
A

A
x
V

G

G

L

V

G
x
W

N
x
Y

F

V

T

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P

E

E

G

N

-

W

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x
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A

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N

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D

D

L

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binding arginine: A39 (≠ G32), I40 (≠ V33), G42 (= G35), T43 (= T36), G44 (= G37), E115 (≠ T108), Y116 (≠ W109), A119 (= A112), F228 (= F220), A229 (= A221), I231 (≠ S223), V314 (≠ A307), S318 (≠ G311)
binding cholesterol: A197 (≠ S192), W201 (≠ G196), Y202 (≠ N197)
binding : G28 (≠ Q21), A29 (≠ T22), F30 (≠ R23), D31 (≠ H24), M34 (= M27), A178 (≠ G183), R179 (≠ G184), A182 (≠ I187), V183 (≠ Q188), A186 (≠ H191), I187 (vs. gap), A190 (vs. gap), L193 (vs. gap), L194 (vs. gap), A197 (≠ S192), V198 (≠ A193), H295 (≠ G288), Q296 (≠ I289), W298 (≠ Y291), V299 (≠ A292), F302 (≠ I295)

5oqtA Crystal structure of a bacterial cationic amino acid transporter (cat) homologue (see paper)
23% identity, 86% coverage: 11:424/479 of query aligns to 16:419/456 of 5oqtA

query
sites
5oqtA

A

A

S

K

A

G

H

A

T

S