SitesBLAST
Comparing PP_0794 FitnessBrowser__Putida:PP_0794 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 19 hits to proteins with known functional sites (download)
2jg1C Structure of staphylococcus aureus d-tagatose-6-phosphate kinase with cofactor and substrate (see paper)
36% identity, 88% coverage: 4:280/315 of query aligns to 7:285/315 of 2jg1C
- active site: G256 (= G251), S257 (≠ A252), G258 (= G253), D259 (= D254)
- binding phosphoaminophosphonic acid-adenylate ester: S227 (= S222), G229 (= G224), A230 (≠ E225), G232 (= G227), I246 (≠ P241), I249 (≠ V244), V251 (= V246), V255 (= V250), G256 (= G251), S257 (≠ A252), G258 (= G253), D259 (= D254), T261 (≠ L256), N283 (≠ T278)
- binding 6-O-phosphono-beta-D-tagatofuranose: D17 (= D14), G42 (= G39), K43 (= K40), R93 (= R90), C95 (≠ N92), L108 (≠ N106), G140 (= G138), S141 (= S139), D259 (= D254)
Sites not aligning to the query:
2jg1A Structure of staphylococcus aureus d-tagatose-6-phosphate kinase with cofactor and substrate (see paper)
36% identity, 88% coverage: 4:280/315 of query aligns to 10:288/318 of 2jg1A
- active site: G259 (= G251), S260 (≠ A252), G261 (= G253), D262 (= D254)
- binding phosphoaminophosphonic acid-adenylate ester: K46 (= K40), N193 (= N186), S230 (= S222), G232 (= G224), G235 (= G227), I252 (≠ V244), V254 (= V246), G259 (= G251), S260 (≠ A252), G261 (= G253), D262 (= D254), T264 (≠ L256), N286 (≠ T278)
Sites not aligning to the query:
2jgvB Structure of staphylococcus aureus d-tagatose-6-phosphate kinase in complex with adp (see paper)
36% identity, 88% coverage: 4:280/315 of query aligns to 6:284/314 of 2jgvB
Sites not aligning to the query:
2f02A Crystal structure of lacc from enterococcus faecalis in complex with atp
32% identity, 97% coverage: 4:309/315 of query aligns to 3:311/319 of 2f02A
- binding adenosine-5'-triphosphate: N186 (= N186), S224 (= S222), G226 (= G224), I243 (≠ P241), I246 (≠ V244), G253 (= G251), S254 (≠ A252), G255 (= G253), T258 (≠ L256), M280 (≠ A279), G283 (≠ A282), M284 (≠ Q283)
3uqdB Crystal structure of the phosphofructokinase-2 from escherichia coli in complex with substrates and products (see paper)
34% identity, 91% coverage: 1:286/315 of query aligns to 1:287/309 of 3uqdB
- active site: G253 (= G251), A254 (= A252), G255 (= G253), D256 (= D254)
- binding adenosine-5'-diphosphate: K185 (= K184), N187 (= N186), S224 (= S222), G226 (= G224), P227 (≠ E225), G229 (= G227), S248 (≠ V246), M258 (≠ L256), V280 (≠ A279), G283 (≠ A282), S284 (≠ Q283)
- binding adenosine-5'-triphosphate: Y23 (≠ R23), K27 (≠ V27)
- binding 6-O-phosphono-beta-D-fructofuranose: K27 (≠ V27), R29 (= R29)
- binding 1,6-di-O-phosphono-beta-D-fructofuranose: D14 (= D14), G39 (= G39), N43 (= N43), R90 (= R90), R105 (≠ D104), S139 (= S139), G253 (= G251)
3uqdA Crystal structure of the phosphofructokinase-2 from escherichia coli in complex with substrates and products (see paper)
34% identity, 91% coverage: 1:286/315 of query aligns to 1:287/309 of 3uqdA
- active site: G253 (= G251), A254 (= A252), G255 (= G253), D256 (= D254)
- binding adenosine-5'-triphosphate: K185 (= K184), S224 (= S222), G226 (= G224), P227 (≠ E225), G229 (= G227), T251 (= T249), G255 (= G253), M258 (≠ L256), V280 (≠ A279), G283 (≠ A282), S284 (≠ Q283), T287 (= T286)
- binding 6-O-phosphono-beta-D-fructofuranose: D14 (= D14), G38 (≠ A38), G39 (= G39), N43 (= N43), R90 (= R90), S139 (= S139), D256 (= D254)
- binding 1,6-di-O-phosphono-beta-D-fructofuranose: K27 (≠ V27), R29 (= R29)
3n1cA Crystal structure of the phosphofructokinase-2 from escherichia coli in complex with fructose-6-phosphate (see paper)
34% identity, 91% coverage: 1:286/315 of query aligns to 1:287/309 of 3n1cA
- active site: G253 (= G251), A254 (= A252), G255 (= G253), D256 (= D254)
- binding 6-O-phosphono-beta-D-fructofuranose: D14 (= D14), K27 (≠ V27), R29 (= R29), G39 (= G39), N43 (= N43), R90 (= R90), G138 (= G138), S139 (= S139), D256 (= D254)
P06999 ATP-dependent 6-phosphofructokinase isozyme 2; ATP-PFK 2; Phosphofructokinase 2; 6-phosphofructokinase isozyme II; Phosphohexokinase 2; EC 2.7.1.11 from Escherichia coli (strain K12) (see 3 papers)
34% identity, 91% coverage: 1:286/315 of query aligns to 1:287/309 of P06999
- K27 (≠ V27) binding ; binding
- KPN 185:187 (= KPN 184:186) binding in other chain
- NQK 187:189 (≠ NTE 186:188) binding in other chain
- E190 (= E189) mutation to Q: Causes a 50-fold decrease in the kcat value and a 15-fold increment in the apparent KM for ATP.
- SLGPQG 224:229 (≠ SAGEQG 222:227) binding in other chain
- S248 (≠ V246) binding in other chain
- S250 (= S248) binding
- V252 (= V250) binding
- V280 (≠ A279) binding in other chain
- S284 (≠ Q283) binding in other chain
- A286 (≠ V285) binding
Sites not aligning to the query:
- 289 binding
- 291 binding
- 293 binding
3cqdA Structure of the tetrameric inhibited form of phosphofructokinase-2 from escherichia coli (see paper)
34% identity, 91% coverage: 1:286/315 of query aligns to 1:287/304 of 3cqdA
- active site: G253 (= G251), A254 (= A252), G255 (= G253), D256 (= D254)
- binding adenosine-5'-triphosphate: Y23 (≠ R23), G26 (≠ Q26), K27 (≠ V27), K185 (= K184), N187 (= N186), N187 (= N186), K189 (≠ E188), S224 (= S222), G226 (= G224), P227 (≠ E225), G229 (= G227), S248 (≠ V246), T251 (= T249), A254 (= A252), G255 (= G253), M258 (≠ L256), V280 (≠ A279), G283 (≠ A282), S284 (≠ Q283), T287 (= T286)
3ie7A The crystal structure of phosphofructokinase (lin2199) from listeria innocua in complex with atp at 1.6a
29% identity, 97% coverage: 4:309/315 of query aligns to 3:307/309 of 3ie7A
- binding adenosine-5'-triphosphate: N188 (= N186), S220 (= S222), G222 (= G224), A223 (≠ E225), G225 (= G227), V242 (= V244), G249 (= G251), A250 (= A252), G251 (= G253), D252 (= D254), S279 (≠ A281), V283 (= V285)
3uqeA Crystal structure of the phosphofructokinase-2 mutant y23d from escherichia coli
34% identity, 91% coverage: 1:286/315 of query aligns to 1:287/307 of 3uqeA
- active site: G253 (= G251), A254 (= A252), G255 (= G253), D256 (= D254)
- binding adenosine-5'-triphosphate: K185 (= K184), N187 (= N186), S224 (= S222), G226 (= G224), P227 (≠ E225), G229 (= G227), S248 (≠ V246), A254 (= A252), G255 (= G253), M258 (≠ L256), V280 (≠ A279), G283 (≠ A282), S284 (≠ Q283), T287 (= T286)
- binding pyrophosphate 2-: N187 (= N186), K189 (≠ E188)
P9WID3 ATP-dependent 6-phosphofructokinase isozyme 2; ATP-PFK 2; Phosphofructokinase 2; Phosphofructokinase B; Phosphohexokinase 2; Tagatose-6-phosphate kinase; EC 2.7.1.11; EC 2.7.1.144 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
32% identity, 83% coverage: 3:264/315 of query aligns to 13:275/339 of P9WID3
Sites not aligning to the query:
- 283 modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
3julA Crystal structure of listeria innocua d-tagatose-6-phosphate kinase bound with substrate
29% identity, 90% coverage: 4:287/315 of query aligns to 3:278/298 of 3julA
2ajrA Crystal structure of possible 1-phosphofructokinase (ec 2.7.1.56) (tm0828) from thermotoga maritima at 2.46 a resolution
30% identity, 83% coverage: 4:265/315 of query aligns to 3:273/320 of 2ajrA
Sites not aligning to the query:
Q8ZKR2 Aminoimidazole riboside kinase; AIRs kinase; EC 2.7.1.223 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
26% identity, 80% coverage: 39:289/315 of query aligns to 31:292/319 of Q8ZKR2
- G31 (= G39) binding
- Y101 (≠ A119) binding
- R162 (≠ Q177) binding
- A180 (vs. gap) binding
- A181 (vs. gap) binding
- A183 (vs. gap) binding
- G213 (= G215) binding
- D246 (≠ S248) binding
- T248 (≠ V250) binding
- D252 (= D254) binding
- A287 (= A284) binding
- A290 (≠ Q287) binding
- G292 (= G289) binding
Sites not aligning to the query:
1tz3A Crystal structure of aminoimidazole riboside kinase complexed with aminoimidazole riboside (see paper)
25% identity, 80% coverage: 39:289/315 of query aligns to 27:281/299 of 1tz3A
- active site: F88 (≠ R117), G238 (= G251), A239 (= A252), G240 (= G253), D241 (= D254)
- binding 5-aminoimidazole ribonucleoside: G27 (= G39), L83 (≠ K94), F88 (≠ R117), Y90 (≠ A119), R151 (≠ Q177), M154 (≠ P180), D241 (= D254)
Sites not aligning to the query:
1tz6A Crystal structure of aminoimidazole riboside kinase from salmonella enterica complexed with aminoimidazole riboside and atp analog (see paper)
25% identity, 82% coverage: 31:289/315 of query aligns to 16:281/297 of 1tz6A
- active site: C24 (vs. gap), F88 (≠ R117), G238 (= G251), A239 (= A252), G240 (= G253), D241 (= D254)
- binding phosphomethylphosphonic acid adenylate ester: N149 (≠ G175), K176 (vs. gap), E181 (≠ N200), S209 (= S222), G211 (= G224), A212 (≠ E225), G214 (= G227), A239 (= A252), G240 (= G253), F243 (≠ L256), N270 (≠ T278), G273 (≠ A281), A274 (= A282)
- binding 5-aminoimidazole ribonucleoside: G27 (= G39), F88 (≠ R117), Y90 (≠ A119), R151 (≠ Q177), M154 (≠ P180), D241 (= D254)
Sites not aligning to the query:
6ilsB Structure of arabidopsis thaliana ribokinase complexed with ribose and atp (see paper)
26% identity, 96% coverage: 1:301/315 of query aligns to 1:308/313 of 6ilsB
- binding adenosine-5'-triphosphate: N189 (= N186), K225 (≠ S222), G227 (= G224), I246 (≠ P241), A248 (≠ K243), A257 (= A252), G258 (= G253), F261 (≠ L256), A286 (= A282), S287 (≠ Q283)
- binding alpha-D-ribofuranose: N12 (≠ A12), D14 (= D14), G40 (= G39), K41 (= K40), N44 (= N43), E144 (≠ G143), D259 (= D254)
A1A6H3 Ribokinase; AtRBSK; RK; EC 2.7.1.15 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
26% identity, 91% coverage: 14:301/315 of query aligns to 80:374/379 of A1A6H3
Sites not aligning to the query:
- 1:74 mutation Missing: Loss of ribokinase activity.
Query Sequence
>PP_0794 FitnessBrowser__Putida:PP_0794
MAKILTLTLNPALDITIGLDTLRPGQVNRSQAQHSHAAGKGLNVAQVLADLGHSVTVGGF
LGGDNLQPFEALIDGRGFTDCFVRVPGETRSNIKLVEADGRVTDINGQGPEVDEAARSAL
LHRLEQIAPGHDAVVVAGSLPRGISADWFRQLLERLKAQGLKVVLDSSGEALRVGLQSAP
WLVKPNTEELGEVLGLAVDNLTQQRAAAKRLLDSGVEHVVVSAGEQGVSWFSRDLALQAR
PPKVRVASTVGAGDSLVAGMVHGLLLAEAPAQTLTRATAIAAQAVTQVGFGIHDREHLAQ
LEAAVQLTEQQEGCR
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory