SitesBLAST
Comparing PP_1259 FitnessBrowser__Putida:PP_1259 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5oqtA Crystal structure of a bacterial cationic amino acid transporter (cat) homologue (see paper)
30% identity, 80% coverage: 6:435/537 of query aligns to 22:447/456 of 5oqtA
- binding alanine: I38 (= I22), G40 (vs. gap), T41 (vs. gap), G42 (vs. gap), F226 (= F208), A227 (= A209), I229 (≠ L211)
- binding : E24 (≠ Q8), G26 (≠ S10), F28 (≠ L12), D29 (= D13), M32 (≠ F16), A176 (= A154), R177 (≠ K155), A184 (≠ V162), A188 (≠ I166), L192 (= L170), Q294 (≠ R277), V297 (≠ L288)
6f34A Crystal structure of a bacterial cationic amino acid transporter (cat) homologue bound to arginine. (see paper)
30% identity, 80% coverage: 6:435/537 of query aligns to 24:449/458 of 6f34A
- binding arginine: I40 (= I22), G42 (vs. gap), T43 (vs. gap), G44 (vs. gap), E115 (≠ A96), Y116 (≠ F97), A119 (= A107), F228 (= F208), A229 (= A209), I231 (≠ L211), V314 (≠ C303)
- binding cholesterol: W201 (≠ T177), Y202 (≠ F178)
- binding : G28 (≠ S10), F30 (≠ L12), D31 (= D13), M34 (≠ F16), A178 (= A154), R179 (≠ K155), A186 (≠ V162), I187 (≠ F163), A190 (≠ I166), L194 (= L170), Q296 (≠ R277), V299 (≠ L288)
Q88CZ8 L-histidine transporter HutT from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
27% identity, 63% coverage: 6:345/537 of query aligns to 8:336/467 of Q88CZ8
- T27 (≠ S25) mutation T->A,S: Retains 60% of wild-type activity.; mutation to N: Retains 20% of wild-type activity.
- E98 (≠ A96) mutation to A: Retains 80% of wild-type activity.
- K156 (= K164) mutation K->A,Q: Retains less than 10% of wild-type activity.; mutation to R: Retains 40% of wild-type activity.
- F212 (= F208) mutation F->A,Q: Loss of activity.; mutation to Y: No change in activity.
- E218 (≠ T214) mutation E->A,Q: Loss of activity.; mutation to D: Retains 70% of wild-type activity.
P30825 High affinity cationic amino acid transporter 1; CAT-1; CAT1; Ecotropic retroviral leukemia receptor homolog; Ecotropic retrovirus receptor homolog; Solute carrier family 7 member 1; System Y+ basic amino acid transporter from Homo sapiens (Human) (see paper)
25% identity, 73% coverage: 4:393/537 of query aligns to 27:438/629 of P30825
- N226 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine
6f2wA Bacterial asc transporter crystal structure in open to in conformation (see paper)
25% identity, 77% coverage: 7:418/537 of query aligns to 2:401/433 of 6f2wA
P82251 b(0,+)-type amino acid transporter 1; b(0,+)AT1; Glycoprotein-associated amino acid transporter b0,+AT1; Solute carrier family 7 member 9 from Homo sapiens (Human) (see 11 papers)
25% identity, 74% coverage: 2:397/537 of query aligns to 23:409/487 of P82251
- V40 (≠ L19) to M: in CSNU; uncertain significance
- IIGSG 43:47 (≠ IFGSG 22:26) binding L-arginine
- I44 (≠ F23) to T: in CSNU; type I; dbSNP:rs121908485
- S51 (≠ A30) to F: in CSNU; uncertain significance
- P52 (≠ A31) to L: in CSNU; impairs protein stability and dimer formation; dbSNP:rs1198613438
- A70 (≠ F47) to V: in CSNU; partial loss of amino acid transport activity; dbSNP:rs769448665
- Y99 (= Y76) to H: in CSNU; uncertain significance
- G105 (= G82) to R: in CSNU; type III; severe loss of amino acid transport activity; dbSNP:rs121908480
- W114 (≠ F91) to R: in CSNU; uncertain significance
- I120 (≠ F97) to L: in CSNU; uncertain significance
- T123 (≠ S99) to M: in CSNU; partial loss of amino acid transport activity; dbSNP:rs79987078
- V142 (≠ A122) to A: no effect on amino acid transport activity; dbSNP:rs12150889
- C144 (≠ S124) modified: Interchain (with C-114 in SLC3A1)
- V170 (= V150) to M: in CSNU; type III; severe loss of amino acid transport activity; dbSNP:rs121908479
- A182 (≠ V162) to T: in CSNU; type III; partial loss of amino acid transport activity; dbSNP:rs79389353
- G195 (vs. gap) to R: in CSNU; type III; decreased amino acid transport activity; dbSNP:rs121908482
- L223 (≠ I206) to M: slightly decreased amino acid transport activity; dbSNP:rs1007160
- A224 (≠ I207) to V: in CSNU; non-classic type I; dbSNP:rs140873167
- N227 (vs. gap) to D: in CSNU; decreased amino acid transport activity
- W230 (vs. gap) to R: in CSNU; complete loss of amino acid transport activity; mutation to A: Abolishes amino acid transport activity.
- D233 (≠ L211) binding L-arginine; mutation to A: Complete loss of amino acid transport activity.
- W235 (≠ L213) mutation to A: Complete loss of amino acid transport activity.
- Q237 (≠ P215) mutation to A: Reduces amino acid transport activity.
- G259 (≠ S237) to R: in CSNU; type III; impairs protein stability and dimer formation; dbSNP:rs121908483
- P261 (≠ L239) to L: in CSNU; types I and III; dbSNP:rs121908486
- S286 (≠ N263) to F: in CSNU; uncertain significance; dbSNP:rs755135545
- C321 (≠ M308) mutation to S: Does not affect amino acid transport activity.
- A324 (≠ T311) to E: in CSNU; uncertain significance
- V330 (≠ G317) to M: in CSNU; type III; dbSNP:rs201618022
- A331 (≠ W318) to V: in CSNU; non-classic type I; dbSNP:rs768466784
- R333 (≠ Q320) to Q: in CSNU; decreased amino acid transport activity; dbSNP:rs769576205; to W: in CSNU; severe loss of amino acid transport activity; dbSNP:rs121908484
- A354 (= A341) to T: in CSNU; type III; severe loss of amino acid transport activity; dbSNP:rs939028046
- S379 (= S367) mutation to A: Markedly reduces amino acid transport activity.
- A382 (≠ L370) to T: in CSNU; severe loss of amino acid transport activity; dbSNP:rs774878350
- W383 (≠ V371) mutation to A: Complete loss of amino acid transport activity.
- Y386 (= Y374) mutation to A: Loss of amino acid transport activity.
- K401 (≠ P389) to E: in CSNU; uncertain significance; dbSNP:rs760264924
Sites not aligning to the query:
- 426 L → P: in CSNU; uncertain significance
- 482 P → L: in CSNU; severe loss of amino acid transport activity; no effect on localization to the apical membrane; dbSNP:rs146815072; mutation P->A,G,S,V: No effect on amino acid transport activity.; mutation P->F,I,M,W: Decreased amino acid transport activity.
P25737 Lysine-specific permease LysP; Lysine transporter LysP; Trigger transporter LysP from Escherichia coli (strain K12) (see 2 papers)
27% identity, 63% coverage: 6:345/537 of query aligns to 14:352/489 of P25737
- Y102 (≠ T93) mutation to L: Retains 4% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
- W106 (≠ F97) mutation to L: Retains 20% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
- K163 (= K164) mutation to A: Retains 24% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
- F216 (= F208) mutation to L: Retains 13% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
- E222 (≠ T214) mutation to A: Abolishes lysine uptake. Strongly inhibits CadC.
- E230 (= E222) mutation to V: Abolishes lysine uptake. Shows significant less inhibition of CadC.
- D275 (≠ G264) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-278.
- D278 (≠ S267) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-275.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 438 E→A: Retains 14% of wild-type lysine uptake activity. Is unable to inhibit CadC.
- 443 D→A: Retains 11% of wild-type lysine uptake activity. Is unable to inhibit CadC.
- 446 D→A: Retains 13% of wild-type lysine uptake activity. Is unable to inhibit CadC.
6li9B Heteromeric amino acid transporter b0,+at-rbat complex bound with arginine (see paper)
25% identity, 71% coverage: 17:397/537 of query aligns to 9:380/458 of 6li9B
7nf6B Ovine b0,+at-rbat heterodimer (see paper)
25% identity, 73% coverage: 8:397/537 of query aligns to 1:381/455 of 7nf6B
Q9UHI5 Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; hLAT2; Solute carrier family 7 member 8 from Homo sapiens (Human) (see 3 papers)
24% identity, 73% coverage: 6:398/537 of query aligns to 37:423/535 of Q9UHI5
- I53 (= I22) binding L-leucine
- Y93 (= Y60) mutation to A: Nearly complete reduction of glycine, L-alanine, and L-glutamine uptake. Minimal effect on the transport of L-isoleucine, L-histidine and L-tryptophan.
- N134 (≠ S99) Important for substrate specificity; binding L-tryptophan; mutation to Q: Reduces L-leucine uptake activity. Abolishes L-tryptophan uptake.; mutation to S: The substrate specificity changed dramatically reducing L-glutamine, glycine and L-alanine uptake activity thus mimicking the selectivity of SLC7A5.
- C154 (≠ L119) modified: Interchain (with C-210 in SLC3A2)
- W174 (≠ F144) mutation to A: Does not affect protein expression, plasma membrane localization, or L-alanine uptake.
- F243 (= F208) mutation to A: Abolishes leucine and tryptophan transport activities.
- G246 (≠ L211) Important for substrate specificity; binding L-leucine; mutation to S: Strong decrease in the uptake of large substrates L-tryptophan, L-glutamine, and L-histidine but increases the uptake of small neutral amino acids glycine and L-alanine.
- V302 (≠ A266) to I: found in a patient with age-related hearing loss; does not affect L-alanine transport activity. Decreases L-tyrosine transport activity; dbSNP:rs142951280
- N395 (≠ L370) binding L-tryptophan; mutation to Q: Strongly reduces L-leucine uptake activity. Strongly reduces L-tryptophan uptake activity.
- Y396 (≠ V371) mutation to A: Strongly reduces L-leucine uptake activity.
- T402 (≠ A377) to M: found in a patient with age-related hearing loss; strongly decreased L-alanine transport activity. Decreases L-tyrosine transport activity; dbSNP:rs758342760
- R418 (= R393) to C: found in a patient with age-related hearing loss; decreases L-alanine transport activity. Decreases L-tyrosine transport activity; dbSNP:rs146946494
Sites not aligning to the query:
- 460 V → E: found in a patient with age-related hearing loss; strongly decreases L-alanine transport activity. Decreases L-tyrosine transport activity. Decreases cell membrane localization; dbSNP:rs2048595742
P46349 Gamma-aminobutyric acid permease; GABA permease; 4-aminobutyrate permease; Gamma-aminobutyrate permease; Proline transporter GabP from Bacillus subtilis (strain 168) (see paper)
23% identity, 73% coverage: 6:398/537 of query aligns to 9:397/469 of P46349
- G33 (≠ A30) mutation to D: Lack of activity.
- G42 (= G39) mutation to S: Lack of activity.
- G301 (≠ Y307) mutation to V: Lack of activity.
- G338 (vs. gap) mutation to E: Lack of activity.
- F341 (≠ W343) mutation to S: Lack of activity.
Sites not aligning to the query:
- 414 G→R: Lack of activity.
Q9QXW9 Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; mLAT2; Solute carrier family 7 member 8 from Mus musculus (Mouse) (see paper)
24% identity, 73% coverage: 6:397/537 of query aligns to 36:421/531 of Q9QXW9