SitesBLAST
Comparing PP_1649 FitnessBrowser__Putida:PP_1649 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5z20F The ternary structure of d-lactate dehydrogenase from pseudomonas aeruginosa with nadh and oxamate (see paper)
77% identity, 96% coverage: 14:340/342 of query aligns to 8:334/336 of 5z20F
- active site: S108 (= S114), R241 (= R247), D265 (= D271), E270 (= E276), H302 (= H308)
- binding 1,4-dihydronicotinamide adenine dinucleotide: Y107 (= Y113), G160 (= G166), Q161 (= Q167), I162 (= I168), Y180 (= Y186), D181 (= D187), P182 (= P188), C212 (= C218), P213 (= P219), T218 (= T224), T239 (= T245), G240 (= G246), R241 (= R247), H302 (= H308), A304 (= A310)
4cukA Structure of salmonella d-lactate dehydrogenase in complex with nadh
53% identity, 94% coverage: 14:334/342 of query aligns to 1:321/330 of 4cukA
- active site: S101 (= S114), R234 (= R247), D258 (= D271), E263 (= E276), H295 (= H308)
- binding 1,4-dihydronicotinamide adenine dinucleotide: Y100 (= Y113), G153 (= G166), K154 (≠ Q167), I155 (= I168), F173 (≠ Y186), D174 (= D187), P175 (= P188), H204 (= H217), C205 (= C218), P206 (= P219), N211 (≠ T224), T232 (= T245), Y260 (= Y273), H295 (= H308), A297 (= A310)
5z21B The ternary structure of d-lactate dehydrogenase from fusobacterium nucleatum with nadh and oxamate (see paper)
46% identity, 94% coverage: 15:336/342 of query aligns to 3:324/331 of 5z21B
- active site: S101 (= S114), R235 (= R247), D259 (= D271), E264 (= E276), H296 (= H308)
- binding 1,4-dihydronicotinamide adenine dinucleotide: Y100 (= Y113), I105 (≠ V118), G153 (= G166), K154 (≠ Q167), I155 (= I168), D174 (= D187), L175 (≠ P188), P207 (= P219), T212 (= T224), T233 (= T245), G234 (= G246), R235 (= R247), H296 (= H308), Y299 (≠ F311)
4zgsA Identification of the pyruvate reductase of chlamydomonas reinhardtii (see paper)
47% identity, 96% coverage: 10:336/342 of query aligns to 7:339/346 of 4zgsA
- active site: S111 (= S114), R244 (= R247), D268 (= D271), E273 (= E276), H311 (= H308)
- binding nicotinamide-adenine-dinucleotide: Y110 (= Y113), G163 (= G166), A164 (≠ Q167), I165 (= I168), D184 (= D187), C215 (= C218), P216 (= P219), L218 (≠ T221), S220 (≠ H223), T221 (= T224), S243 (≠ G246), H311 (= H308), F314 (= F311)
8grvA Dictyostelium discoideum lactate dehydrogenase (dicldha)with NAD
41% identity, 92% coverage: 14:327/342 of query aligns to 3:317/336 of 8grvA
- binding nicotinamide-adenine-dinucleotide: V106 (= V118), G154 (= G166), N155 (≠ Q167), I156 (= I168), D176 (= D187), I177 (≠ P188), I178 (≠ Y189), T208 (≠ C218), P209 (= P219), T214 (= T224), V235 (≠ T245), H298 (= H308), A300 (= A310), W301 (≠ F311)
2dldA D-lactate dehydrogenase complexed with nadh and oxamate
37% identity, 85% coverage: 38:327/342 of query aligns to 27:316/337 of 2dldA
- active site: S103 (= S114), R236 (= R247), D260 (= D271), E265 (= E276), H297 (= H308)
- binding 1,4-dihydronicotinamide adenine dinucleotide: T154 (= T165), G155 (= G166), H156 (≠ Q167), I157 (= I168), D176 (= D187), I177 (≠ P188), V207 (≠ C218), P208 (= P219), N213 (≠ T224), C234 (≠ T245), S235 (≠ G246), H297 (= H308)
Sites not aligning to the query:
P30901 D-lactate dehydrogenase; D-LDH; D-specific 2-hydroxyacid dehydrogenase; EC 1.1.1.28 from Lactobacillus helveticus (Lactobacillus suntoryeus) (see paper)
37% identity, 85% coverage: 38:327/342 of query aligns to 27:316/337 of P30901
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
P26297 D-lactate dehydrogenase; D-LDH; D-specific 2-hydroxyacid dehydrogenase; EC 1.1.1.28 from Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081 / BCRC 10696 / JCM 1002 / NBRC 13953 / NCIMB 11778 / NCTC 12712 / WDCM 00102 / Lb 14) (see 2 papers)
37% identity, 85% coverage: 38:327/342 of query aligns to 27:316/333 of P26297
- HI 156:157 (≠ QI 167:168) binding
- D176 (= D187) binding
- H206 (= H217) mutation to Q: Increase of activity.
- VP 207:208 (≠ CP 218:219) binding
- N213 (≠ T224) binding
- R236 (= R247) mutation to K: Decrease of activity.
- D260 (= D271) binding ; mutation to N: Decrease of activity.
- E265 (= E276) mutation to Q: Decrease of activity.
- H297 (= H308) mutation to Q: 90% loss of activity.
1j49A Insights into domain closure, substrate specificity and catalysis of d-lactate dehydrogenase from lactobacillus bulgaricus (see paper)
37% identity, 85% coverage: 38:327/342 of query aligns to 27:316/332 of 1j49A
- active site: S103 (= S114), R236 (= R247), D260 (= D271), E265 (= E276), H297 (= H308)
- binding nicotinamide-adenine-dinucleotide: Y102 (= Y113), I107 (≠ V118), G153 (= G164), G155 (= G166), I157 (= I168), Y175 (= Y186), D176 (= D187), I177 (≠ P188), V207 (≠ C218), P208 (= P219), N213 (≠ T224), V234 (≠ T245), S235 (≠ G246), R236 (= R247), H297 (= H308), A299 (= A310), F300 (= F311)
3kb6B Crystal structure of d-lactate dehydrogenase from aquifex aeolicus complexed with NAD and lactic acid (see paper)
36% identity, 83% coverage: 59:342/342 of query aligns to 44:332/334 of 3kb6B
- active site: S97 (= S114), R231 (= R247), D255 (= D271), E260 (= E276), H294 (= H308)
- binding lactic acid: F49 (= F64), S72 (= S89), V73 (≠ A90), G74 (= G91), Y96 (= Y113), R231 (= R247), H294 (= H308)
- binding nicotinamide-adenine-dinucleotide: V73 (≠ A90), Y96 (= Y113), V101 (= V118), G150 (= G166), R151 (≠ Q167), I152 (= I168), D171 (= D187), V172 (≠ P188), P203 (= P219), T229 (= T245), A230 (≠ G246), R231 (= R247), H294 (= H308), A296 (= A310), Y297 (≠ F311)
P17584 D-2-hydroxyisocaproate dehydrogenase; D-HICDH; EC 1.1.1.- from Lacticaseibacillus paracasei (Lactobacillus paracasei) (see paper)
32% identity, 89% coverage: 14:317/342 of query aligns to 1:304/333 of P17584
1dxyA Structure of d-2-hydroxyisocaproate dehydrogenase (see paper)
32% identity, 89% coverage: 14:317/342 of query aligns to 1:304/330 of 1dxyA
- active site: S101 (= S114), R234 (= R247), D258 (= D271), E263 (= E276), H295 (= H308)
- binding 2-oxo-4-methylpentanoic acid: V77 (≠ A90), Y100 (= Y113), Y298 (≠ F311)
- binding nicotinamide-adenine-dinucleotide: Y100 (= Y113), G152 (= G164), G154 (= G166), H155 (≠ Q167), I156 (= I168), Y174 (= Y186), D175 (= D187), P176 (= P188), H204 (= H217), V205 (≠ C218), P206 (= P219), N211 (≠ T224), T232 (= T245), A233 (≠ G246), R234 (= R247), H295 (= H308), Y298 (≠ F311)
4prlA Crystal structure of d-lactate dehydrogenase with NAD+ from lactobacillus jensenii (see paper)
36% identity, 80% coverage: 70:342/342 of query aligns to 58:329/330 of 4prlA
- binding nicotinamide-adenine-dinucleotide: Y101 (= Y113), I106 (≠ V118), V154 (≠ T165), G155 (= G166), H156 (≠ Q167), I157 (= I168), Y175 (= Y186), D176 (= D187), H205 (= H217), T206 (≠ C218), P207 (= P219), A233 (≠ T245), A234 (≠ G246), D259 (= D271), H295 (= H308), A297 (= A310)
2yq5C Crystal structure of d-isomer specific 2-hydroxyacid dehydrogenase from lactobacillus delbrueckii ssp. Bulgaricus: NAD complexed form (see paper)
34% identity, 89% coverage: 38:340/342 of query aligns to 25:330/331 of 2yq5C
- active site: S102 (= S114), R236 (= R247), D260 (= D271), E265 (= E276), H297 (= H308)
- binding nicotinamide-adenine-dinucleotide: Y101 (= Y113), I106 (≠ V118), V155 (≠ T165), G156 (= G166), H157 (≠ Q167), I158 (= I168), Y176 (= Y186), D177 (= D187), V178 (≠ P188), H206 (= H217), T207 (≠ C218), P208 (= P219), A235 (≠ G246), R236 (= R247), H297 (= H308), F300 (= F311)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
34% identity, 82% coverage: 59:340/342 of query aligns to 47:321/334 of 5aovA
- active site: L100 (≠ S114), R241 (= R247), D265 (= D271), E270 (= E276), H288 (= H308)
- binding glyoxylic acid: M52 (≠ F64), L53 (≠ I65), L53 (≠ I65), Y74 (≠ R88), A75 (≠ S89), V76 (≠ A90), G77 (= G91), R241 (= R247), H288 (= H308)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ A90), T104 (≠ V118), F158 (≠ T165), G159 (= G166), R160 (≠ Q167), I161 (= I168), S180 (≠ D187), R181 (≠ P188), A211 (≠ H217), V212 (≠ C218), P213 (= P219), T218 (= T224), I239 (≠ T245), A240 (≠ G246), R241 (= R247), H288 (= H308), G290 (≠ A310)
4xkjA A novel d-lactate dehydrogenase from sporolactobacillus sp
34% identity, 92% coverage: 14:328/342 of query aligns to 1:315/332 of 4xkjA
- active site: S102 (= S114), R234 (= R247), D258 (= D271), E263 (= E276), H295 (= H308)
- binding nicotinamide-adenine-dinucleotide: Y101 (= Y113), V106 (= V118), G152 (= G164), G154 (= G166), R155 (≠ Q167), I156 (= I168), D175 (= D187), I176 (≠ P188), R179 (≠ N191), H204 (= H217), V205 (≠ C218), P206 (= P219), T211 (= T224), A232 (≠ T245), R234 (= R247), H295 (= H308), G297 (≠ A310), F298 (= F311)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
39% identity, 70% coverage: 78:316/342 of query aligns to 60:286/304 of 1wwkA
- active site: S96 (= S114), R230 (= R247), D254 (= D271), E259 (= E276), H278 (= H308)
- binding nicotinamide-adenine-dinucleotide: V100 (= V118), G146 (= G164), F147 (≠ T165), G148 (= G166), R149 (≠ Q167), I150 (= I168), Y168 (= Y186), D169 (= D187), P170 (= P188), V201 (≠ C218), P202 (= P219), T207 (= T224), T228 (= T245), S229 (≠ G246), D254 (= D271), H278 (= H308), G280 (≠ A310)
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
35% identity, 78% coverage: 59:325/342 of query aligns to 46:307/332 of 6biiA
- active site: L99 (≠ S114), R240 (= R247), D264 (= D271), E269 (= E276), H287 (= H308)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (≠ A90), T103 (≠ V118), G156 (= G164), F157 (≠ T165), G158 (= G166), R159 (≠ Q167), I160 (= I168), A179 (≠ D187), R180 (≠ P188), S181 (≠ Y189), K183 (≠ N191), V211 (≠ C218), P212 (= P219), E216 (≠ H223), T217 (= T224), V238 (≠ T245), A239 (≠ G246), R240 (= R247), D264 (= D271), H287 (= H308), G289 (vs. gap)
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
39% identity, 73% coverage: 69:318/342 of query aligns to 54:288/525 of 3ddnB
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
39% identity, 73% coverage: 69:318/342 of query aligns to 53:287/526 of 3dc2A
Sites not aligning to the query:
Query Sequence
>PP_1649 FitnessBrowser__Putida:PP_1649
MTHPRHALQRSSTMRALLFSSQHYDQESFTKAAGGTALELHFQPARLTLDTAALADGFEV
VCAFINDELDAPVLQRLAAAGTRLIALRSAGYNHVDLAAAQRLGLAVVRVPAYSPHAVAE
HAVALILALNRRLHRAYNRTREGDFTLHGLTGFDLHGKTVGVVGTGQIGVAFARIMAGFG
CQLLAYDPYPNPELLALGARYLPLPELLREARIISLHCPLTEHTRHLINAQSLAQLQPGA
MLINTGRGALVDTPALIDALKSGQLGYLGLDVYEEEAQLFFEDRSDLPLQDDVLARLLTF
PNVIITAHQAFLTREALDAIAATTLDNINRWAAGNPQNLVMG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory