SitesBLAST
Comparing PP_1817 PP_1817 Oxidoreductase, short-chain dehydrogenase/reductase family to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7djsD Crystal structure of isopiperitenol dehydrogenase from pseudomonas aeruginosa complexed with NAD
84% identity, 98% coverage: 5:253/253 of query aligns to 3:251/251 of 7djsD
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), G16 (= G18), I17 (= I19), D36 (= D38), L37 (= L39), C61 (= C63), D62 (= D64), V63 (= V65), N89 (= N91), A90 (= A92), T140 (= T142), S142 (= S144), Y155 (= Y157), K159 (= K161), A186 (= A188), V187 (= V189)
6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
44% identity, 98% coverage: 5:253/253 of query aligns to 3:248/248 of 6ixmC
- active site: G16 (= G18), S142 (= S144), Y155 (= Y157), K159 (= K161)
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), S15 (≠ A17), G16 (= G18), I17 (= I19), D36 (= D38), I37 (≠ L39), A61 (≠ C63), D62 (= D64), T63 (≠ V65), N89 (= N91), A90 (= A92), M140 (≠ T142), S142 (= S144), Y155 (= Y157), K159 (= K161), P185 (= P187), A186 (= A188), Y187 (≠ V189), I188 (= I190), L192 (≠ M194)
4urfB Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
43% identity, 98% coverage: 3:249/253 of query aligns to 1:244/248 of 4urfB
- active site: G16 (= G18), S142 (= S144), I152 (≠ M154), Y155 (= Y157), K159 (= K161)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: L210 (≠ V215), R211 (≠ G216), R212 (= R217)
- binding bicarbonate ion: I92 (= I94), G94 (≠ I96), R109 (≠ A111), R179 (= R181), S228 (= S233)
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), G14 (= G16), N15 (≠ A17), G16 (= G18), I17 (= I19), D36 (= D38), I37 (≠ L39), D62 (= D64), T63 (≠ V65), N89 (= N91), A90 (= A92), G91 (= G93), I140 (≠ T142), Y155 (= Y157), K159 (= K161), P185 (= P187), A186 (= A188), I188 (= I190), T190 (= T192)
4urfA Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
43% identity, 98% coverage: 3:249/253 of query aligns to 1:244/248 of 4urfA
- active site: G16 (= G18), S142 (= S144), I152 (≠ M154), Y155 (= Y157), K159 (= K161)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: I92 (= I94), S93 (≠ E95), G94 (≠ I96), E95 (= E97), T97 (≠ H99), E101 (= E103), T103 (≠ S105), Q106 (≠ E108), R109 (≠ A111), S175 (≠ K177), G177 (= G179)
- binding magnesium ion: S237 (≠ H242), Y238 (≠ C243)
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), G14 (= G16), N15 (≠ A17), G16 (= G18), I17 (= I19), D36 (= D38), I37 (≠ L39), W41 (≠ G43), D62 (= D64), T63 (≠ V65), N89 (= N91), A90 (= A92), G91 (= G93), I140 (≠ T142), Y155 (= Y157), K159 (= K161), P185 (= P187), I188 (= I190), T190 (= T192)
4ureB Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
43% identity, 98% coverage: 3:249/253 of query aligns to 1:244/248 of 4ureB
- active site: G16 (= G18), S142 (= S144), I152 (≠ M154), Y155 (= Y157), K159 (= K161)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: N15 (≠ A17), G16 (= G18), I17 (= I19), N89 (= N91), G91 (= G93), Y155 (= Y157), P185 (= P187), A186 (= A188)
Q9LBG2 Levodione reductase; (6R)-2,2,6-trimethyl-1,4-cyclohexanedione reductase; EC 1.1.1.- from Leifsonia aquatica (Corynebacterium aquaticum) (see paper)
39% identity, 98% coverage: 5:252/253 of query aligns to 11:265/267 of Q9LBG2
- 17:42 (vs. 11:36, 54% identical) binding
- E103 (= E95) mutation E->A,D,N,Q: 26-fold increase in Km and a much lower enantiomeric excess of the reaction products.
1iy8A Crystal structure of levodione reductase (see paper)
39% identity, 98% coverage: 5:252/253 of query aligns to 2:256/258 of 1iy8A
- active site: G15 (= G18), S143 (= S144), Q153 (≠ M154), Y156 (= Y157), K160 (= K161)
- binding nicotinamide-adenine-dinucleotide: G11 (= G14), S14 (≠ A17), G15 (= G18), L16 (≠ I19), D35 (= D38), V36 (≠ L39), A62 (≠ C63), D63 (= D64), V64 (= V65), N90 (= N91), G92 (= G93), I93 (= I94), T141 (= T142), S143 (= S144), Y156 (= Y157), K160 (= K161), P186 (= P187), G187 (≠ A188), T191 (= T192), P192 (≠ D193), M193 (= M194)
4wecA Crystal structure of a short chain dehydrogenase from mycobacterium smegmatis
37% identity, 100% coverage: 1:252/253 of query aligns to 4:252/258 of 4wecA
- active site: G21 (= G18), S143 (= S144), Q154 (≠ M154), Y157 (= Y157), K161 (= K161)
- binding nicotinamide-adenine-dinucleotide: G17 (= G14), A19 (≠ G16), S20 (≠ A17), G21 (= G18), I22 (= I19), D41 (= D38), I42 (≠ L39), V61 (≠ C63), D62 (= D64), V63 (= V65), N89 (= N91), T141 (= T142), Y157 (= Y157), K161 (= K161), P187 (= P187), P189 (≠ V189), V190 (≠ I190)
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
40% identity, 100% coverage: 1:252/253 of query aligns to 1:254/255 of 5itvA
- active site: G18 (= G18), S141 (= S144), Y154 (= Y157), K158 (= K161)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G14), S17 (≠ A17), G18 (= G18), I19 (= I19), D38 (= D38), I39 (≠ L39), T61 (≠ C63), I63 (≠ V65), N89 (= N91), G91 (= G93), T139 (= T142), S141 (= S144), Y154 (= Y157), K158 (= K161), P184 (= P187), G185 (≠ A188), I186 (≠ V189), I187 (= I190)
4qecA Elxo with NADP bound (see paper)
34% identity, 94% coverage: 11:249/253 of query aligns to 6:244/248 of 4qecA
- active site: G13 (= G18), N111 (= N116), S139 (= S144), Y152 (= Y157), K156 (= K161)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: K12 (≠ A17), G13 (= G18), I14 (= I19), S33 (≠ D38), R34 (≠ D40), K38 (≠ G44), D59 (= D64), V60 (= V65), N86 (= N91), A87 (= A92), G88 (= G93), I137 (≠ T142), Y152 (= Y157), K156 (= K161), P182 (= P187), I185 (= I190)
7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
39% identity, 98% coverage: 2:249/253 of query aligns to 3:244/247 of 7tzpG
- binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G14), R18 (≠ A17), G19 (= G18), I20 (= I19), D39 (= D38), R40 (≠ L39), C63 (= C63), I65 (≠ V65), N91 (= N91), G93 (= G93), I94 (= I94), V114 (= V115), Y155 (= Y157), K159 (= K161), I188 (= I190), T190 (= T192), T193 (≠ F195)
5itvD Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
39% identity, 100% coverage: 1:252/253 of query aligns to 1:226/227 of 5itvD
- active site: G18 (= G18), S141 (= S144), Y154 (= Y157), K158 (= K161)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G14), S17 (≠ A17), G18 (= G18), I19 (= I19), D38 (= D38), I39 (≠ L39), T61 (≠ C63), D62 (= D64), I63 (≠ V65), N89 (= N91), T139 (= T142), S141 (= S144), Y154 (= Y157), K158 (= K161), P184 (= P187), G185 (≠ A188), I187 (= I190)
2dtxA Structure of thermoplasma acidophilum aldohexose dehydrogenase (aldt) in complex with d-mannose (see paper)
41% identity, 96% coverage: 8:249/253 of query aligns to 8:244/255 of 2dtxA
2dteA Structure of thermoplasma acidophilum aldohexose dehydrogenase (aldt) in complex with nadh (see paper)
41% identity, 96% coverage: 8:249/253 of query aligns to 8:244/255 of 2dteA
- active site: G18 (= G18), S132 (= S144), Y145 (= Y157), S148 (= S160), K149 (= K161)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G14), S16 (≠ G16), M17 (≠ A17), G18 (= G18), I19 (= I19), S38 (≠ Q50), I39 (= I51), C52 (= C63), D53 (= D64), V54 (= V65), N80 (= N91), A81 (= A92), I130 (≠ T142), S132 (= S144), Y145 (= Y157), K149 (= K161), P174 (= P187), A175 (= A188), T176 (≠ V189), I177 (= I190), T179 (= T192), P180 (≠ D193), L181 (≠ M194), V182 (≠ F195)
4nbwA Crystal structure of fabg from plesiocystis pacifica (see paper)
42% identity, 96% coverage: 8:249/253 of query aligns to 2:248/253 of 4nbwA
- active site: G12 (= G18), S146 (= S144), Y159 (= Y157), K163 (= K161)
- binding nicotinamide-adenine-dinucleotide: G8 (= G14), N11 (≠ A17), G12 (= G18), I13 (= I19), D32 (= D38), L33 (= L39), V57 (≠ C63), D58 (= D64), V59 (= V65), N85 (= N91), A86 (= A92), G87 (= G93), S146 (= S144), Y159 (= Y157), K163 (= K161), I192 (= I190), T194 (= T192)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
40% identity, 96% coverage: 8:249/253 of query aligns to 8:241/244 of 4nbuB
- active site: G18 (= G18), N111 (= N116), S139 (= S144), Q149 (≠ M154), Y152 (= Y157), K156 (= K161)
- binding acetoacetyl-coenzyme a: D93 (≠ Q98), K98 (≠ E103), S139 (= S144), N146 (≠ A151), V147 (≠ P152), Q149 (≠ M154), Y152 (= Y157), F184 (≠ V189), M189 (= M194), K200 (≠ F208)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G14), N17 (≠ A17), G18 (= G18), I19 (= I19), D38 (= D38), F39 (≠ L39), V59 (≠ C63), D60 (= D64), V61 (= V65), N87 (= N91), A88 (= A92), G89 (= G93), I90 (= I94), T137 (= T142), S139 (= S144), Y152 (= Y157), K156 (= K161), P182 (= P187), F184 (≠ V189), T185 (≠ I190), T187 (= T192), M189 (= M194)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
39% identity, 98% coverage: 3:249/253 of query aligns to 1:244/247 of 4jroC
- active site: G16 (= G18), S142 (= S144), Q152 (≠ M154), Y155 (= Y157), K159 (= K161)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G14), S14 (≠ G16), R15 (≠ A17), G16 (= G18), I17 (= I19), N35 (≠ A37), Y36 (vs. gap), N37 (≠ D38), G38 (≠ L39), S39 (≠ D40), N63 (≠ D64), V64 (= V65), N90 (= N91), A91 (= A92), I93 (= I94), I113 (≠ V115), S142 (= S144), Y155 (= Y157), K159 (= K161), P185 (= P187), I188 (= I190), T190 (= T192)
6zzsD Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and 3-oxovalerate (see paper)
37% identity, 100% coverage: 1:252/253 of query aligns to 1:260/261 of 6zzsD
- active site: G18 (= G18), S143 (= S144), Y156 (= Y157)
- binding nicotinamide-adenine-dinucleotide: G14 (= G14), S17 (≠ A17), I19 (= I19), D38 (= D38), M39 (≠ L39), D64 (= D64), V65 (= V65), N91 (= N91), A92 (= A92), G93 (= G93), M141 (≠ T142), A142 (= A143), S143 (= S144), Y156 (= Y157), K160 (= K161), P186 (= P187), G187 (≠ A188), V189 (≠ I190), T191 (= T192), L193 (≠ M194)
- binding 3-oxidanylidenepentanoic acid: Q95 (= Q98), S143 (= S144), N145 (≠ A146), K153 (≠ M154), Y156 (= Y157), Q197 (vs. gap)
P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
42% identity, 97% coverage: 5:249/253 of query aligns to 4:243/247 of P73574
- A14 (= A15) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
- P151 (= P152) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
- K160 (= K161) mutation to A: Almost no activity on acetoacetyl-CoA.
- F188 (≠ V189) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
- N198 (≠ K205) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.
5h5xC Crystal structure of nadh bound carbonyl reductase from streptomyces coelicolor
42% identity, 98% coverage: 5:253/253 of query aligns to 11:257/257 of 5h5xC
- active site: G24 (= G18), S151 (= S144), Y164 (= Y157), K168 (= K161)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G20 (= G14), S23 (≠ A17), G24 (= G18), I25 (= I19), D44 (= D38), F45 (≠ L39), L69 (≠ C63), D70 (= D64), N97 (= N91), A98 (= A92), Y164 (= Y157), K168 (= K161), P194 (= P187), G195 (≠ A188), I197 (= I190), T199 (= T192)
Query Sequence
>PP_1817 PP_1817 Oxidoreductase, short-chain dehydrogenase/reductase family
MSMTFSGQVALVTGAGAGIGRATALAFAHEGMKVVVADLDPVGGEATVAQIHAAGGEALF
IACDVTRDAEVRQLHERLMAAYGRLDYAFNNAGIEIEQHRLAEGSEAEFDAIMGVNVKGV
WLCMKYQLPLLLAQGGGAIVNTASVAGLGAAPKMSIYSASKHAVIGLTKSAAIEYAKKGI
RVNAVCPAVIDTDMFRRAYQADPRKAEFAAAMHPVGRIGKVEEIASAVLYLCSDGAAFTT
GHCLTVDGGATAI
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory