SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PP_1972 FitnessBrowser__Putida:PP_1972 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3fslA Crystal structure of tyrosine aminotransferase tripple mutant (p181q, r183g,a321k) from escherichia coli at 2.35 a resolution
51% identity, 99% coverage: 3:398/398 of query aligns to 1:397/397 of 3fslA

query
sites
3fslA

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active site: W131 (= W132), D212 (= D213), A214 (= A215), K247 (= K248)
binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: G103 (= G104), G104 (= G105), S105 (≠ T106), W131 (= W132), H134 (= H135), N184 (= N185), D212 (= D213), A214 (= A215), Y215 (= Y216), S244 (= S245), S246 (= S247), K247 (= K248), R255 (= R256)

3tatA Tyrosine aminotransferase from e. Coli (see paper)
51% identity, 99% coverage: 3:398/398 of query aligns to 1:397/397 of 3tatA

query
sites
3tatA

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active site: W131 (= W132), D212 (= D213), A214 (= A215), K247 (= K248)
binding pyridoxal-5'-phosphate: G103 (= G104), G104 (= G105), S105 (≠ T106), W131 (= W132), H179 (= H180), N184 (= N185), D212 (= D213), A214 (= A215), Y215 (= Y216), S244 (= S245), S246 (= S247), K247 (= K248), R255 (= R256)

5vwrA E.Coli aspartate aminotransferase-(1r,3s,4s)-3-amino-4- fluorocyclopentane-1-carboxylic acid (fcp)-alpha-ketoglutarate (see paper)
47% identity, 99% coverage: 3:398/398 of query aligns to 1:396/396 of 5vwrA

query
sites
5vwrA

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active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K248)
binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-glutamic acid: I13 (= I15), L14 (= L16), I33 (≠ V35), G34 (= G36), G102 (= G104), G103 (= G105), T104 (= T106), W130 (= W132), H178 (= H180), N183 (= N185), D211 (= D213), A213 (= A215), Y214 (= Y216), S243 (= S245), S245 (= S247), K246 (= K248), R254 (= R256), F348 (= F350), R374 (= R376)

5t4lA Plp and gaba trigger gabr-mediated transcription regulation in bacillus subsidies via external aldimine formation (see paper)
47% identity, 99% coverage: 3:398/398 of query aligns to 1:396/396 of 5t4lA

query
sites
5t4lA

L

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D

P

E

R

R

T

P

D

G

K

K

V

I

N

N

L

L

G

G

V

I

G
|
G

V

V

Y

Y

C

K

N

D

E

E

E

T

G

G

R

K

I

T

P

P

L

V

L

L

R

T

A

S

V

V

I

K

E

K

A

A

E

E

T

Q

Q

Y

R

L

A

L

A

E

Q

N

H

E

A

T

S

T

R

K

G

N

Y

Y

L

L

P

G

I

I

D

D

G

G

I

I

A

P

T

E

Y

F

D

G

Q

R

A

C

V

T

Q

Q

K

E

L

L

F

F

G

G

A

K

E

G

S

S

P

A

L

L

L

I

A

N

A

D

G

K

R

R

V

A

V

R

T

T

V

A

Q

Q

A

T

V

P

G
|
G

T
|
T

G

G

A

A

L

L

K

R

I

V

G

A

A

A

D

D

F

F

L

L

K

A

R

K

L

N

S

T

P

S

N

V

A

K

V

R

V

V

A

W

I

V

S

S

D

N

P

P

S

S

W
|
W

E

P

N

N

H
|
H

R

K

A

S

L

V

F

F

E

N

S

S

A

A

G

G

F

L

P

E

V

V

Q

R

T

E

Y

Y

R

A

Y

Y

D

D

A

A

P

E

T

N

N

H

D

T

V

L

N

D

R

F

A

D

G

A

M

L

L

I

E

N

D

S

L

L

N

N

A

E

L

A

P

Q

S

A

G

G

S

D

I

V

V

V

V

L

L

F

H
|
H

A

G

C

C

H

H

N
|
N

P

P

T

T

G

G

V

I

D

D

L

P

N

T

L

L

D

E

D

Q

W

W

K

Q

N

T

V

L

L

A

E

Q

V

L

V

S

K

V

A

E

K

K

G

G

H

W

V

L

P

P

F

L

L

F

D
|
D

M

F

A
|
A

Y
|
Y

Q

Q

G

G

F

F

G

A

D

R

G

G

I

L

A

E

E

E

D

D

A

A

F

E

A

G

V

L

R

R

L

A

F

F

A

A

E

A

S

M

G

H

L

K

E

E

F

L

F

I

V

V

S

A

S

S

S
|
S

F

Y

S
|
S

K
|
K

S

N

F

F

S

G

L

L

Y

Y

G

N

E

E

R
|
R

V

V

G

G

A

A

L

C

S

T

I

L

V

V

T

A

A

A

S

D

K

S

D

E

E

T

S

V

T

D

R

R

V

A

L

F

S

S

Q

Q

V

M

K

K

R

A

V

A

I

I

R

R

T

A

T

N

Y

Y

S

S

N

N

P

P

T

A

H

H

G

G

A

A

T

S

I

V

V

V

A

A

T

T

V

I

L

L

N

S

S

N

A

D

E

A

L

L

R

R

Q

A

M

I

W

W

E

E

T

Q

E

E

L

L

A

T

E

D

M

M

R

R

E

Q

R

R

I

I

H

Q

G

R

M

M

R

R

K

Q

Q

L

M

F

V

V

S

N

L

T

L

L

A

Q

E

E

Y

K

G

G

A

A

N

N

R

R

D

D

F

F

S

S

F

F

V

I

G

I

R

K

Q

Q

V

N

G

G

M

M

F

F

S

S

Y

F

S

S

G

G

L

L

T

T

V

K

E

E

Q

Q

V

V

T

L

R

R

L

L

K

R

N

E

D

E

F

F

G

G

I

V

Y

Y

A

A

L

V

D

A

T

S

G

G

R

R

I

V

A

N

V

V

A

A

A

G

L

M

N

T

Q

P

S

D

N

N

I

M

H

A

V

P

V

L

T

C

K

E

A

A

I

I

V

V

E

A

V

V

L

L

active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K248)
binding (4R)-4-amino-6-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}hexanoic acid: I13 (= I15), G34 (= G36), G102 (= G104), G103 (= G105), T104 (= T106), W130 (= W132), H133 (= H135), H178 (= H180), N183 (= N185), D211 (= D213), A213 (= A215), Y214 (= Y216), S243 (= S245), S245 (= S247), K246 (= K248), R254 (= R256)

3qpgA Crystal structures of escherichia coli aspartate aminotransferase reconstituted with 1-deaza-pyridoxal 5'-phosphate: internal aldimine and stable l-aspartate external aldimine (see paper)
47% identity, 99% coverage: 3:398/398 of query aligns to 1:396/396 of 3qpgA

query
sites
3qpgA

L

M

F

F

S

E

A

N

V

I

E

T

L

A

A

A

P

P

R

A

D

D

P

P

I
|
I

L
|
L

G

G

L

L

N

A

E

D

A

L

F

F

N

R

A

A

D

D

P

E

R

R

T

P

D

G

K

K

V

I

N

N

L

L

G

G

V
x
I

G
|
G

V

V

Y

Y

C

K

N

D

E

E

E

T

G

G

R

K

I

T

P

P

L

V

L

L

R

T

A

S

V

V

I

K

E

K

A

A

E

E

T

Q

Q

Y

R

L

A

L

A

E

Q

N

H

E

A

T

S

T

R

K

G

N

Y

Y

L

L

P

G

I

I

D

D

G

G

I

I

A

P

T

E

Y

F

D

G

Q

R

A

C

V

T

Q

Q

K

E

L

L

F

F

G

G

A

K

E

G

S

S

P

A

L

L

L

I

A

N

A

D

G

K

R

R

V

A

V

R

T

T

V

A

Q

Q

A

T

V

P

G
|
G

T
|
T

G

G

A

A

L

L

K

R

I

V

G

A

A

A

D

D

F

F

L

L

K

A

R

K

L

N

S

T

P

S

N

V

A

K

V

R

V

V

A

W

I

V

S

S

D

N

P

P

S

S

W
|
W

E

P

N

N

H
|
H

R

K

A

S

L

V

F

F

E

N

S

S

A

A

G

G

F

L

P

E

V

V

Q

R

T

E

Y

Y

R

A

Y

Y

D

D

A

A

P

E

T

N

N

H

D

T

V

L

N

D

R

F

A

D

G

A

M

L

L

I

E

N

D

S

L

L

N

N

A

E

L

A

P

Q

S

A

G

G

S

D

I

V

V

V

V

L

L

F

H

H

A

G

C

C

H

H

N
|
N

P

P

T

T

G

G

V

I

D

D

L

P

N

T

L

L

D

E

D

Q

W

W

K

Q

N

T

V

L

L

A

E

Q

V

L

V

S

K

V

A

E

K

K

G

G

H

W

V

L

P

P

F

L

L

F

D
|
D

M

F

A
|
A

Y
|
Y

Q

Q

G

G

F

F

G

A

D

R

G

G

I

L

A

E

E

E

D

D

A

A

F

E

A

G

V

L

R

R

L

A

F

F

A

A

E

A

S

M

G

H

L

K

E

E

F

L

F

I

V

V

S

A

S

S

S
|
S

F

Y

S
|
S

K
|
K

S

N

F

F

S

G

L

L

Y

Y

G

N

E

E

R
|
R

V

V

G

G

A

A

L

C

S

T

I

L

V

V

T

A

A

A

S

D

K

S

D

E

E

T

S

V

T

D

R

R

V

A

L

F

S

S

Q

Q

V

M

K

K

R

A

V

A

I

I

R

R

T

A

T

N

Y

Y

S

S

N

N

P

P

T

A

H

H

G

G

A

A

T

S

I

V

V

V

A

A

T

T

V

I

L

L

N

S

S

N

A

D

E

A

L

L

R

R

Q

A

M

I

W

W

E

E

T

Q

E

E

L

L

A

T

E

D

M

M

R

R

E

Q

R

R

I

I

H

Q

G

R

M

M

R

R

K

Q

Q

L

M

F

V

V

S

N

L

T

L

L

A

Q

E

E

Y

K

G

G

A

A

N

N

R

R

D

D

F

F

S

S

F

F

V

I

G

I

R

K

Q

Q

V

N

G

G

M

M

F

F

S

S

Y

F

S

S

G

G

L

L

T

T

V

K

E

E

Q

Q

V

V

T

L

R

R

L

L

K

R

N

E

D

E

F

F

G

G

I

V

Y

Y

A

A

L

V

D

A

T

S

G

G

R
|
R

I

V

A

N

V

V

A

A

A

G

L

M

N

T

Q

P

S

D

N

N

I

M

H

A

V

P

V

L

T

C

K

E

A

A

I

I

V

V

E

A

V

V

L

L

active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K248)
binding (E)-N-{2-hydroxy-3-methyl-6-[(phosphonooxy)methyl]benzylidene}-L-aspartic acid: I13 (= I15), L14 (= L16), I33 (≠ V35), G34 (= G36), G102 (= G104), G103 (= G105), T104 (= T106), W130 (= W132), H133 (= H135), N183 (= N185), D211 (= D213), A213 (= A215), Y214 (= Y216), S243 (= S245), S245 (= S247), K246 (= K248), R254 (= R256), R374 (= R376)

3pa9A Mechanism of inactivation of e. Coli aspartate aminotransferase by (s)-4-amino-4,5-dihydro-2-furancarboxylic acid (s-adfa) ph 7.5 (see paper)
47% identity, 99% coverage: 3:398/398 of query aligns to 1:396/396 of 3pa9A

query
sites
3pa9A

L

M

F

F

S

E

A

N

V

I

E

T

L

A

A

A

P

P

R

A

D

D

P

P

I

I

L

L

G

G

L

L

N

A

E

D

A

L

F

F

N

R

A

A

D

D

P

E

R

R

T

P

D

G

K

K

V

I

N

N

L

L

G

G

V

I

G
|
G

V

V

Y

Y

C

K

N

D

E

E

E

T

G

G

R

K

I

T

P

P

L

V

L

L

R

T

A

S

V

V

I

K

E

K

A

A

E

E

T

Q

Q

Y

R

L

A

L

A

E

Q

N

H

E

A

T

S

T

R

K

G

N

Y

Y

L

L

P

G

I

I

D

D

G

G

I

I

A

P

T

E

Y

F

D

G

Q

R

A

C

V

T

Q

Q

K

E

L

L

F

F

G

G

A

K

E

G

S

S

P

A

L

L

L

I

A

N

A

D

G

K

R

R

V

A

V

R

T

T

V

A

Q

Q

A

T

V

P

G
|
G

T
|
T

G

G

A

A

L

L

K

R

I

V

G

A

A

A

D

D

F

F

L

L

K

A

R

K

L

N

S

T

P

S

N

V

A

K

V

R

V

V

A

W

I

V

S

S

D

N

P

P

S

S

W
|
W

E

P

N

N

H
|
H

R

K

A

S

L

V

F

F

E

N

S

S

A

A

G

G

F

L

P

E

V

V

Q

R

T

E

Y

Y

R

A

Y

Y

D

D

A

A

P

E

T

N

N

H

D

T

V

L

N

D

R

F

A

D

G

A

M

L

L

I

E

N

D

S

L

L

N

N

A

E

L

A

P

Q

S

A

G

G

S

D

I

V

V

V

V

L

L

F

H
|
H

A

G

C

C

H

H

N
|
N

P

P

T

T

G

G

V

I

D

D

L

P

N

T

L

L

D

E

D

Q

W

W

K

Q

N

T

V

L

L

A

E

Q

V

L

V

S

K

V

A

E

K

K

G

G

H

W

V

L

P

P

F

L

L

F

D
|
D

M

F

A
|
A

Y
|
Y

Q

Q

G

G

F

F

G

A

D

R

G

G

I

L

A

E

E

E

D

D

A

A

F

E

A

G

V

L

R

R

L

A

F

F

A

A

E

A

S

M

G

H

L

K

E

E

F

L

F

I

V

V

S

A

S

S

S
|
S

F

Y

S
|
S

K
|
K

S

N

F

F

S

G

L

L

Y

Y

G

N

E

E

R
|
R

V

V

G

G

A

A

L

C

S

T

I

L

V

V

T

A

A

A

S

D

K

S

D

E

E

T

S

V

T

D

R

R

V

A

L

F

S

S

Q

Q

V

M

K

K

R

A

V

A

I

I

R

R

T

A

T

N

Y

Y

S

S

N

N

P

P

T

A

H

H

G

G

A

A

T

S

I

V

V

V

A

A

T

T

V

I

L

L

N

S

S

N

A

D

E

A

L

L

R

R

Q

A

M

I

W

W

E

E

T

Q

E

E

L

L

A

T

E

D

M

M

R

R

E

Q

R

R

I

I

H

Q

G

R

M

M

R

R

K

Q

Q

L

M

F

V

V

S

N

L

T

L

L

A

Q

E

E

Y

K

G

G

A

A

N

N

R

R

D

D

F

F

S

S

F

F

V

I

G

I

R

K

Q

Q

V

N

G

G

M

M

F
|
F

S

S

Y

F

S

S

G

G

L

L

T

T

V

K

E

E

Q

Q

V

V

T

L

R

R

L

L

K

R

N

E

D

E

F

F

G

G

I

V

Y

Y

A

A

L

V

D

A

T

S

G

G

R
|
R

I

V

A

N

V

V

A

A

A

G

L

M

N

T

Q

P

S

D

N

N

I

M

H

A

V

P

V

L

T

C

K

E

A

A

I

I

V

V

E

A

V

V

L

L

active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K248)
binding 4-aminofuran-2-carboxylic acid: G34 (= G36), W130 (= W132), N183 (= N185), Y214 (= Y216), K246 (= K248), F348 (= F350), R374 (= R376)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G102 (= G104), G103 (= G105), T104 (= T106), W130 (= W132), H133 (= H135), H178 (= H180), N183 (= N185), D211 (= D213), A213 (= A215), Y214 (= Y216), S243 (= S245), S245 (= S247), K246 (= K248), R254 (= R256)

1x2aA Crystal structure of e.Coli aspat complexed with n-phosphopyridoxyl-d- glutamic acid (see paper)
47% identity, 99% coverage: 3:398/398 of query aligns to 1:396/396 of 1x2aA

query
sites
1x2aA

L

M

F

F

S

E

A

N

V

I

E

T

L

A

A

A

P

P

R

A

D

D

P

P

I
|
I

L
|
L

G

G

L

L

N

A

E

D

A

L

F

F

N

R

A

A

D

D

P

E

R

R

T

P

D

G

K

K

V

I

N

N

L

L

G

G

V
x
I

G
|
G

V

V

Y

Y

C

K

N

D

E

E

E

T

G

G

R

K

I

T

P

P

L

V

L

L

R

T

A

S

V

V

I

K

E

K

A

A

E

E

T

Q

Q

Y

R

L

A

L

A

E

Q

N

H

E

A

T

S

T

R

K

G

N

Y
|
Y

L

L

P

G

I

I

D

D

G

G

I

I

A

P

T

E

Y

F

D

G

Q

R

A

C

V

T

Q

Q

K

E

L

L

F

F

G

G

A

K

E

G

S

S

P

A

L

L

L

I

A

N

A

D

G

K

R

R

V

A

V

R

T

T

V

A

Q

Q

A

T

V

P

G
|
G

T
|
T

G

G

A

A

L

L

K

R

I

V

G

A

A

A

D

D

F

F

L

L

K

A

R

K

L

N

S

T

P

S

N

V

A

K

V

R

V

V

A

W

I

V

S

S

D

N

P

P

S

S

W
|
W

E

P

N

N

H

H

R

K

A

S

L

V

F

F

E

N

S

S

A

A

G

G

F

L

P

E

V

V

Q

R

T

E

Y

Y

R

A

Y

Y

D

D

A

A

P

E

T

N

N

H

D

T

V

L

N

D

R

F

A

D

G

A

M

L

L

I

E

N

D

S

L

L

N

N

A

E

L

A

P

Q

S

A

G

G

S

D

I

V

V

V

V

L

L

F

H
|
H

A

G

C

C

H

H

N
|
N

P

P

T

T

G

G

V

I

D

D

L

P

N

T

L

L

D

E

D

Q

W

W

K

Q

N

T

V

L

L

A

E

Q

V

L

V

S

K

V

A

E

K

K

G

G

H

W

V

L

P

P

F

L

L

F

D
|
D

M

F

A
|
A

Y
|
Y

Q

Q

G

G

F

F

G

A

D

R

G

G

I

L

A

E

E

E

D

D

A

A

F

E

A

G

V

L

R

R

L

A

F

F

A

A

E

A

S

M

G

H

L

K

E

E

F

L

F

I

V

V

S

A

S

S

S
|
S

F

Y

S
|
S

K
|
K

S

N

F

F

S

G

L

L

Y

Y

G

N

E

E

R
|
R

V

V

G

G

A

A

L

C

S

T

I

L

V

V

T

A

A

A

S

D

K

S

D

E

E

T

S

V

T

D

R

R

V

A

L

F

S

S

Q

Q

V

M

K

K

R

A

V

A

I

I

R
|
R

T

A

T

N

Y

Y

S

S

N

N

P

P

T

A

H

H

G

G

A

A

T

S

I

V

V

V

A

A

T

T

V

I

L

L

N

S

S

N

A

D

E

A

L

L

R

R

Q

A

M

I

W

W

E

E

T

Q

E

E

L

L

A

T

E

D

M

M

R

R

E

Q

R

R

I

I

H

Q

G

R

M

M

R

R

K

Q

Q

L

M

F

V

V

S

N

L

T

L

L

A

Q

E

E

Y

K

G

G

A

A

N

N

R

R

D

D

F

F

S

S

F

F

V

I

G

I

R

K

Q

Q

V

N

G

G

M

M

F
|
F

S

S

Y

F

S

S

G

G

L

L

T

T

V

K

E

E

Q

Q

V

V

T

L

R

R

L

L

K

R

N

E

D

E

F

F

G

G

I

V

Y

Y

A

A

L

V

D

A

T

S

G

G

R
|
R

I

V

A

N

V

V

A

A

A

G

L

M

N

T

Q

P

S

D

N

N

I

M

H

A

V

P

V

L

T

C

K

E

A

A

I

I

V

V

E

A

V

V

L

L

active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K248)
binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-d-glutamic acid: I13 (= I15), L14 (= L16), I33 (≠ V35), G34 (= G36), Y65 (= Y67), G102 (= G104), G103 (= G105), T104 (= T106), W130 (= W132), H178 (= H180), N183 (= N185), D211 (= D213), A213 (= A215), Y214 (= Y216), S243 (= S245), S245 (= S247), K246 (= K248), R254 (= R256), R280 (= R282), F348 (= F350), R374 (= R376)

1x29A Crystal structure of e.Coli aspat complexed with n-phosphopyridoxyl-2- methyl-l-glutamic acid (see paper)
47% identity, 99% coverage: 3:398/398 of query aligns to 1:396/396 of 1x29A

query
sites
1x29A

L

M

F

F

S

E

A

N

V

I

E

T

L

A

A

A

P

P

R

A

D

D

P

P

I
|
I

L
|
L

G

G

L

L

N

A

E

D

A

L

F

F

N

R

A

A

D

D

P

E

R

R

T

P

D

G

K

K

V

I

N

N

L

L

G

G

V
x
I

G
|
G

V

V

Y

Y

C

K

N

D

E

E

E

T

G

G

R

K

I

T

P

P

L

V

L

L

R

T

A

S

V

V

I

K

E

K

A

A

E

E

T

Q

Q

Y

R

L

A

L

A

E

Q

N

H

E

A

T

S

T

R

K

G

N

Y
|
Y

L

L

P

G

I

I

D

D

G

G

I

I

A

P

T

E

Y

F

D

G

Q

R

A

C

V

T

Q

Q

K

E

L

L

F

F

G

G

A

K

E

G

S

S

P

A

L

L

L

I

A

N

A

D

G

K

R

R

V

A

V

R

T

T

V

A

Q

Q

A

T

V

P

G
|
G

T
|
T

G

G

A

A

L

L

K

R

I

V

G

A

A

A

D

D

F

F

L

L

K

A

R

K

L

N

S

T

P

S

N

V

A

K

V

R

V

V

A

W

I

V

S

S

D

N

P

P

S

S

W
|
W

E

P

N

N

H
|
H

R

K

A

S

L

V

F

F

E

N

S

S

A

A

G

G

F

L

P

E

V

V

Q

R

T

E

Y

Y

R

A

Y

Y

D

D

A

A

P

E

T

N

N

H

D

T

V

L

N

D

R

F

A

D

G

A

M

L

L

I

E

N

D

S

L

L

N

N

A

E

L

A

P

Q

S

A

G

G

S

D

I

V

V

V

V

L

L

F

H
|
H

A

G

C

C

H

H

N
|
N

P

P

T

T

G

G

V

I

D

D

L

P

N

T

L

L

D

E

D

Q

W

W

K

Q

N

T

V

L

L

A

E

Q

V

L

V

S

K

V

A

E

K

K

G

G

H

W

V

L

P

P

F

L

L

F

D
|
D

M

F

A
|
A

Y
|
Y

Q

Q

G

G

F

F

G

A

D

R

G

G

I

L

A

E

E

E

D

D

A

A

F

E

A

G

V

L

R

R

L

A

F

F

A

A

E

A

S

M

G

H

L

K

E

E

F

L

F

I

V

V

S

A

S

S

S
|
S

F

Y

S
|
S

K
|
K

S

N

F

F

S

G

L

L

Y

Y

G

N

E

E

R
|
R

V

V

G

G

A

A

L

C

S

T

I

L

V

V

T

A

A

A

S

D

K

S

D

E

E

T

S

V

T

D

R

R

V

A

L

F

S

S

Q

Q

V

M

K

K

R

A

V

A

I

I

R
|
R

T

A

T

N

Y

Y

S

S

N

N

P

P

T

A

H

H

G

G

A

A

T

S

I

V

V

V

A

A

T

T

V

I

L

L

N

S

S

N

A

D

E

A

L

L

R

R

Q

A

M

I

W

W

E

E

T

Q

E

E

L

L

A

T

E

D

M

M

R

R

E

Q

R

R

I

I

H

Q

G

R

M

M

R

R

K

Q

Q

L

M

F

V

V

S

N

L

T

L

L

A

Q

E

E

Y

K

G

G

A

A

N

N

R

R

D

D

F

F

S

S

F

F

V

I

G

I

R

K

Q

Q

V

N

G

G

M

M

F
|
F

S

S

Y

F

S

S

G

G

L

L

T

T

V

K

E

E

Q

Q

V

V

T

L

R

R

L

L

K

R

N

E

D

E

F

F

G

G

I

V

Y

Y

A

A

L

V

D

A

T

S

G

G

R
|
R

I

V

A

N

V

V

A

A

A

G

L

M

N

T

Q

P

S

D

N

N

I

M

H

A

V

P

V

L

T

C

K

E

A

A

I

I

V

V

E

A

V

V

L

L

active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K248)
binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-2-methyl-l-glutamic acid: I13 (= I15), L14 (= L16), I33 (≠ V35), G34 (= G36), Y65 (= Y67), G102 (= G104), G103 (= G105), T104 (= T106), W130 (= W132), H133 (= H135), H178 (= H180), N183 (= N185), D211 (= D213), A213 (= A215), Y214 (= Y216), S243 (= S245), S245 (= S247), K246 (= K248), R254 (= R256), R280 (= R282), F348 (= F350), R374 (= R376)

1x28A Crystal structure of e.Coli aspat complexed with n-phosphopyridoxyl-l- glutamic acid (see paper)
47% identity, 99% coverage: 3:398/398 of query aligns to 1:396/396 of 1x28A

query
sites
1x28A

L

M

F

F

S

E

A

N

V

I

E

T

L

A

A

A

P

P

R

A

D

D

P

P

I
|
I

L

L

G

G

L

L

N

A

E

D

A

L

F

F

N

R

A

A

D

D

P

E

R

R

T

P

D

G

K

K

V

I

N

N

L

L

G

G

V
x
I

G
|
G

V

V

Y

Y

C

K

N

D

E

E

E

T

G

G

R

K

I

T

P

P

L

V

L

L

R

T

A

S

V

V

I

K

E

K

A

A

E

E

T

Q

Q

Y

R

L

A

L

A

E

Q

N

H

E

A

T

S

T

R

K

G

N

Y
|
Y

L

L

P

G

I

I

D

D

G

G

I

I

A

P

T

E

Y

F

D

G

Q

R

A

C

V

T

Q

Q

K

E

L

L

F

F

G

G

A

K

E

G

S

S

P

A

L

L

L

I

A

N

A

D

G

K

R

R

V

A

V

R

T

T

V

A

Q

Q

A

T

V

P

G
|
G

T
|
T

G

G

A

A

L

L

K

R

I

V

G

A

A

A

D

D

F

F

L

L

K

A

R

K

L

N

S

T

P

S

N

V

A

K

V

R

V

V

A

W

I

V

S

S

D

N

P

P

S

S

W
|
W

E

P

N

N

H
|
H

R

K

A

S

L

V

F

F

E

N

S

S

A

A

G

G

F

L

P

E

V

V

Q

R

T

E

Y

Y

R

A

Y

Y

D

D

A

A

P

E

T

N

N

H

D

T

V

L

N

D

R

F

A

D

G

A

M

L

L

I

E

N

D

S

L

L

N

N

A

E

L

A

P

Q

S

A

G

G

S

D

I

V

V

V

V

L

L

F

H

H

A

G

C

C

H

H

N
|
N

P

P

T

T

G

G

V

I

D

D

L

P

N

T

L

L

D

E

D

Q

W

W

K

Q

N

T

V

L

L

A

E

Q

V

L

V

S

K

V

A

E

K

K

G

G

H

W

V

L

P

P

F

L

L

F

D
|
D

M

F

A
|
A

Y
|
Y

Q

Q

G

G

F

F

G

A

D

R

G

G

I

L

A

E

E

E

D

D

A

A

F

E

A

G

V

L

R

R

L

A

F

F

A

A

E

A

S

M

G

H

L

K

E

E

F

L

F

I

V

V

S

A

S

S

S
|
S

F

Y

S
|
S

K
|
K

S

N

F

F

S

G

L

L

Y

Y

G

N

E

E

R
|
R

V

V

G

G

A

A

L

C

S

T

I

L

V

V

T

A

A

A

S

D

K

S

D

E

E

T

S

V

T

D

R

R

V

A

L

F

S

S

Q

Q

V

M

K

K

R

A

V

A

I

I

R
|
R

T

A

T

N

Y

Y

S

S

N

N

P

P

T

A

H

H

G

G

A

A

T

S

I

V

V

V

A

A

T

T

V

I

L

L

N

S

S

N

A

D

E

A

L

L

R

R

Q

A

M

I

W

W

E

E

T

Q

E

E

L

L

A

T

E

D

M

M

R

R

E

Q

R

R

I

I

H

Q

G

R

M

M

R

R

K

Q

Q

L

M

F

V

V

S

N

L

T

L

L

A

Q

E

E

Y

K

G

G

A

A

N

N

R

R

D

D

F

F

S

S

F

F

V

I

G

I

R

K

Q

Q

V

N

G

G

M

M

F
|
F

S

S

Y

F

S

S

G

G

L

L

T

T

V

K

E

E

Q

Q

V

V

T

L

R

R

L

L

K

R

N

E

D

E

F

F

G

G

I

V

Y

Y

A

A

L

V

D

A

T

S

G

G

R
|
R

I

V

A

N

V

V

A

A

A

G

L

M

N

T

Q

P

S

D

N

N

I

M

H

A

V

P

V

L

T

C

K

E

A

A

I

I

V

V

E

A

V

V

L

L

active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K248)
binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: I13 (= I15), I33 (≠ V35), G34 (= G36), Y65 (= Y67), G102 (= G104), G103 (= G105), T104 (= T106), W130 (= W132), H133 (= H135), N183 (= N185), D211 (= D213), A213 (= A215), Y214 (= Y216), S243 (= S245), S245 (= S247), K246 (= K248), R254 (= R256), R280 (= R282), F348 (= F350), R374 (= R376)

1cq8A Aspartate aminotransferase (E.C. 2.6.1.1) complexed with c6-pyridoxal- 5p-phosphate (see paper)
47% identity, 99% coverage: 3:398/398 of query aligns to 1:396/396 of 1cq8A

query
sites
1cq8A

L

M

F

F

S

E

A

N

V

I

E

T

L

A

A

A

P

P

R

A

D

D

P

P

I
|
I

L

L

G

G

L

L

N

A

E

D

A

L

F

F

N

R

A

A

D

D

P

E

R

R

T

P

D

G

K

K

V

I

N

N

L

L

G

G

V
x
I

G
|
G

V

V

Y

Y

C

K

N

D

E

E

E

T

G

G

R

K

I

T

P

P

L

V

L

L

R

T

A

S

V

V

I

K

E

K

A

A

E

E

T

Q

Q

Y

R

L

A

L

A

E

Q

N

H

E

A

T

S

T

R

K

G

N

Y

Y

L

L

P

G

I

I

D

D

G

G

I

I

A

P

T

E

Y

F

D

G

Q

R

A

C

V

T

Q

Q

K

E

L

L

F

F

G

G

A

K

E

G

S

S

P

A

L

L

L

I

A

N

A

D

G

K

R

R

V

A

V

R

T

T

V

A

Q

Q

A

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V

P

G
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G

T
|
T

G

G

A

A

L

L

K

R

I

V

G

A

A

A

D

D

F

F

L

L

K

A

R

K

L

N

S

T

P

S

N

V

A

K

V

R

V

V

A

W

I

V

S

S

D

N

P

P

S

S

W
|
W

E

P

N

N

H

H

R

K

A

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L

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F

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S

A

A

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G

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V

V

Q

R

T

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Y

Y

R

A

Y

Y

D

D

A

A

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N

N

H

D

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V

L

N

D

R

F

A

D

G

A

M

L

L

I

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N

D

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L

L

N

N

A

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L

A

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A

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G

S

D

I

V

V

V

V

L

L

F

H

H

A

G

C

C

H

H

N
|
N

P

P

T

T

G

G

V

I

D

D

L

P

N

T

L

L

D

E

D

Q

W

W

K

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N

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V

L

L

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L

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K

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G

H

W

V

L

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P

F

L

L

F

D
|
D

M

F

A
|
A

Y
|
Y

Q

Q

G

G

F

F

G

A

D

R

G

G

I

L

A

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E

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D

D

A

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L

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|
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|
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K
|
K

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N

F

F

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L

L

Y

Y

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|
R

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V

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G

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A

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V

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A

A

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K

S

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Q

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K

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A

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I

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Y

Y

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N

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P

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H

H

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G

A

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V

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A

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W

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N

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G

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M

F
|
F

S

S

Y

F

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G

L

L

T

T

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K

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Q

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V

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L

L

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N

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F

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G

I

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Y

Y

A

A

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A

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G

R
|
R

I

V

A

N

V

V

A

A

A

G

L

M

N

T

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P

S

D

N

N

I

M

H

A

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V

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T

C

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E

A

A

I

I

V

V

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A

V

V

L

L

active site: W130 (= W132), D211 (= D213), A213 (= A215), K246 (= K248)
binding 2-[o-phosphonopyridoxyl]-amino-hexanoic acid: I13 (= I15), I33 (≠ V35), G34 (= G36), G102 (= G104), G103 (= G105), T104 (= T106), W130 (= W132), N183 (= N185), D211 (= D213), A213 (= A215), Y214 (= Y216), S243 (= S245), S245 (= S247), K246 (= K248), R254 (= R256), F348 (= F350), R374 (= R376)

1cq7A Aspartate aminotransferase (E.C. 2.6.1.1) complexed with c5-pyridoxal- 5p-phosphate (see paper)
47% identity, 99% coverage: 3:398/398 of query aligns to 1:396/396 of 1cq7A

query
sites
1cq7A

L

M

F

F

S

E

A

N

V

I

E

T

L

A

A

A

P

P

R

A

D

D

P

P

I
|
I

L

L

G

G

L

L

N

A

E

D

A

L

F

F

N

R

A

A

D

D

P

E

R

R

T

P

D

G

K

K

V

I

N

N

L

L

G

G

V
x
I

G
|
G

V

V

Y

Y

C

K

N

D

E

E

E

T

G

G

R

K

I

T

P

P

L

V

L

L

R

T

A

S

V

V

I

K

E

K

A

A

E

E

T

Q

Q

Y

R

L

A

L

A

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N

H

E

A

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T

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K

G

N

Y

Y

L

L

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G

I

I

D

D

G

G

I

I

A

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T

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Y

F

D

G

Q

R

A

C

V

T

Q

Q

K

E

L

L

F

F

G

G

A

K

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A

L

L

L

I

A

N

A

D

G

K

R

R

V

A

V

R

T

T

V

A

Q

Q

A

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P

G
|
G

T
|
T

G

G

A

A

L

L

K

R

I

V

G

A

A

A

D

D

F

F

L

L

K

A

R

K

L

N

S

T

P

S

N

V

A

K

V

R

V

V

A

W

I

V

S

S

D

N

P

P

S

S

W
|
W

E

P

N

N

H

H

R

K

A

S

L

V

F

F

E

N

S

S

A

A

G

G

F

L

P

E

V

V

Q

R

T

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Y

Y

R

A

Y

Y

D

D

A

A

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N

N

H

D

T

V

L

N

D

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F

A

D

G

A

M

L

L

I

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N

D

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L

L

N

N

A

E

L

A

P

Q

S

A

G

G

S

D

I

V

V

V

V

L

L

F

H
|
H

A

G

C

C

H

H

N
|
N

P

P

T

T

G

G

V

I

D

D

L

P

N

T

L

L

D

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D

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W

W

K

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L

L

A

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L

V

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K

V

A

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K

K

G

G

H

W

V

L

P

P

F

L

L

F

D
|
D

M

F

A
|
A

Y
|
Y

Q

Q

G

G

F

F

G

A

D

R

G

G

I

L

A

E

E

E

D

D

A

A

F

E

A

G

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L

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R

L

A

F

F

A

A

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A

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M

G

H

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K

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E

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L

F

I

V

V

S

A

S

S

S
|
S

F

Y

S
|
S

K
|
K

S

N

F

F

S

G

L

L

Y

Y

G

N

E

E

R
|
R

V

V

G

G

A

A

L

C

S

T

I

L

V

V

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A

A

A

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D

K

S

D

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E

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R

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A

L

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Q

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K

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A

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A

I

I

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R

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A

T

N

Y

Y

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S

N

N

P

P

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A

H

H

G

G

A

A

T

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I

V

V

V

A

A

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T

V

I

L

L

N

S

S

N

A

D

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A

L

L

R

R

Q

A

M

I

W

W

E

E

T

Q