SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PP_2052 FitnessBrowser__Putida:PP_2052 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7ocqA Nadh bound to the dehydrogenase domain of the bifunctional mannitol-1- phosphate dehydrogenase/phosphatase mtld from acinetobacter baumannii (see paper)
53% identity, 90% coverage: 1:637/707 of query aligns to 1:642/686 of 7ocqA

query
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7ocqA

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binding magnesium ion: D15 (= D15), D17 (= D17), D175 (= D175)
binding [[(2~{R},3~{S},4~{R},5~{R})-5-(3-aminocarbonyl-4~{H}-pyridin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{S},4~{R},5~{R})-3,4,5-tris(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate: G252 (= G252), A253 (= A253), I254 (= I254), T279 (= T279), R280 (= R280), L335 (= L335), L369 (= L368), N370 (= N369)

7ocnA Crystal structure of the bifunctional mannitol-1-phosphate dehydrogenase/phosphatase mtld from acinetobacter baumannii (see paper)
52% identity, 90% coverage: 1:637/707 of query aligns to 1:646/690 of 7ocnA

query
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7ocnA

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binding magnesium ion: D15 (= D15), D17 (= D17), D175 (= D175)

7ocpA NADPH bound to the dehydrogenase domain of the bifunctional mannitol- 1-phosphate dehydrogenase/phosphatase mtld from acinetobacter baumannii (see paper)
52% identity, 90% coverage: 1:637/707 of query aligns to 1:646/688 of 7ocpA

query
sites
7ocpA

M

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L

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I

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F

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L

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K

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C

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V

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D

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K

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N

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M

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binding magnesium ion: D15 (= D15), D17 (= D17), D175 (= D175)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G250 (= G250), F251 (= F251), A253 (= A253), I254 (= I254), T279 (= T279), R280 (= R280), C334 (≠ S334), L335 (= L335), P336 (= P336), A339 (= A339), L369 (= L368), N370 (= N369)

7ocsA Mannitol-1-phosphate bound to the phosphatase domain of the bifunctional mannitol-1-phosphate dehydrogenase/phosphatase mtld-d16a from acinetobacter baumannii (see paper)
52% identity, 90% coverage: 1:637/707 of query aligns to 1:638/682 of 7ocsA

query
sites
7ocsA

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L

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K

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V

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D

D

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I

I

A

T

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D

I

I

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Q

I

L

I

I

K

K

N

N

R

R

L

L

W

W

N

N

G

G

P

V

H

H

A

A

I

M

V

L

A

A

W

W

Y

Y

A

A

S

S

L

L

L

M

G

G

H

Y

A

E

W

S

V

I

G

G

Q

V

G

A

M

M

G

G

D

D

A

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L

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V

S

K

E

A

L

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A

A

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N

L

L

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Q

A

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V

A

K

P

Q

A

G

L

L

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A

A

I

E

V

Y

L

P

P

Q

N

M

Y

A

A

E

K

V

D

V

L

S

D

R

R

F

M

A

S

E

Q

A

S

F

F

L

L

E

D

R

S

C

C

K

E

T

Y

S

A

F

F

K

K

D

D

P

P

C

C

A

Q

R

R

V

V

G

A

R

R

D

D

P

P

L

L

R

R

K

K

L

L

Q

N

R

H

N

N

E

E

R

R

I

V

L

M

S

A

S

S

I

I

D

A

L

V

A

N

R

I

K

R

H

H

G

D

I

L

A

P

T

Y

P

K

A

N

L

L

A

L

F

K

G

G

A

A

G

A

L

L

A

G

I

Y

H

A

H

Y

A

A

L

I

binding D-Mannitol-1-phosphate: M16 (= M16), D17 (= D17), E24 (= E24), R27 (= R27), L31 (= L31), C51 (≠ S51), L52 (= L52), L54 (= L54), T117 (= T117), S118 (= S118), K150 (= K150)
binding magnesium ion: L372 (= L368), T411 (= T406)

7ocrB NADPH and fructose-6-phosphate bound to the dehydrogenase domain of the bifunctional mannitol-1-phosphate dehydrogenase/phosphatase mtld from acinetobacter baumannii (see paper)
52% identity, 90% coverage: 1:637/707 of query aligns to 1:631/675 of 7ocrB

query
sites
7ocrB

M

V

L

L

F

I

F

F

H

H

G

G

K

K

Q

P

I

V

F

H

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G

A

A

I

I

F

F

D
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D

M

M

D
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D

G

G

T

T

L

M

F

F

D

D

T

T

E

E

R

R

L

L

R

R

F

F

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Q

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L

L

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Q

Q

A

A

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L

Q

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E

I

L

F

I

G

G

K

Q

A

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L

F

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H

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Y

L

L

I

M

G

Q

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C

L

L

G

G

L

L

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S

A

A

K

T

A

A

E

E

A

K

L

L

A

A

K

Q

A

R

H

-

N

-

G

-

E

-

D

-

F

-

P

L

Y

Y

A

K

Q

E

I

I

R

R

Q

K

R

R

A

A

D

D

E

E

L

M

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L

L

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Y

H

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R

N

K

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H

G

G

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P

I

I

K

K

A

K

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G

L

L

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R

L

L

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K

K

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L

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M

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V

A

A

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S

R

R

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A

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I

A

A

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Y

Y

L

L

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I

N

N

A

A

N

N

V

V

L

Y

K

K

Y

F

F

F

D

D

I

V

T

I

V

T

C

C

G

G

D

D

E

E

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V

G

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Q

Q

G

G

K

K

P

P

H

H

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P

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F

F

L

L

K

K

A

A

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S

A

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L

L

N

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C

L

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P

A

G

N

Q

Q

C

C

F

L

M

M

V

F

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D
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D

S

S

E

E

N

N

G

G

M

L

L

T

S

S

A

A

M

H

R

T

A

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K

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G

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L

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I

I

L

L

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D

I

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K

K

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A

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P

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K

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K

A

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N

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Q

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G

G

I

I

H

H

G
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G

F

F

G
|
G

A
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A

I
|
I

G

G

Y

Y

L

I

T

A

Q

Q

V

I

F

L

S

S

H

H

W

W

D

D

G

G

Y

Y

T

T

R

K

P

P

R

K

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R

I

I

A

A

A

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T
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T

R
|
R

S

N

R

S

M

L

L

F

R

R

E

E

S

A

V

V

S

N

A

A

F

F

G

G

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T

Y

Y

S

S

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I

R

R

Y

Y

G

G

A

Q

T

F

S

S

F

Y

D

D

Q

E

T

R

I

I

D

E

N

N

V

M

R

S

M

I

I

V

D

D

M

S

D

D

D

N

D

E

D

Q

A

Q

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M

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L

S

E

M

M

Y

Y

T

T

T

H

A

S

E

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L

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G

A

L

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C

L
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L

P
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P

E

E

Q

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A
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A

I

I

R

E

S

S

Q

E

A

S

R

K

V

I

I

I

A

A

Q

K

G

G

L

L

L

Y

Q

A

R

R

F

F

E

N

R

S

R

Q

G

-

R

E

E

P

L

L

T

T

L

F

L

L

I

I

V

I

L
|
L

N
|
N

K

K

V

V

G

G

G

A

A

K

A

Y

F

L

V

V

R

M

R

K

H

H

V

L

-

K

Q

E

A

A

E

L

L

L

S

E

L

L

L

T

C

N

P

D

P

E

A

D

I

V

S

T

E

E

Q

H

V

I

L

L

Q

K

K

E

T

H

H

Y

F

F

A

C

E

D

T

T

V

V

S
x
N

R

R

I

M

V
|
V

S

S

K

K

L

L

G

S

N

N

D

Q

A

N

L

L

V

Y

R

R

Q

Q

L

L

R

R

I

I

K

K

S

H

Q

N

M

F

F

L

Q

E

N

Q

S

H

L

L

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E

D

D

-

V

E

E

P

E

A

D

S

C

T

N

R

K

K

L

S

T

S

P

T

D

P

Q

L

L

P

N

E

Q

Y

A

E

S

R

I

L

Y

I

V

G

D

H

N

F

M

R

R

P

R

F

N

A

F

Q

Q

P

P

S

G

S

H

A

I

M

L

S

Q

Q

S

L

M

H

D

L

L

V

I

L

L

F

F

N

H

S

S

E

E

A

T

D

D

M

M

P

P

L

I

Y

Y

A

V

E

E

R

K

G

G

S

S

D

P

L

L

E

E

R

K

L

L

R

R

Q

Q

V

V

K

V

T

L

V

V

P

D

D

Q

I

I

A

T

Q

D

I

I

Q

Q

I

L

I

I

K
|
K

N

N

R

R

L

L

W

W

N
|
N

G

G

P

V

H
|
H

A

A

I

M

V

L

A

A

W

W

Y

Y

A

A

S

S

L

L

L

M

G

G

H

Y

A

E

W

S

V

I

G

G

Q

V

G

A

M

M

G

G

D

D

A

H

R

L

V

V

S

K

E

A

L

F

A

A

E

E

R

N

L

L

I

I

R

-

Q

A

E

E

V

V

A

K

P

Q

A

G

L

L

V

A

A

I

E

V

Y

L

P

P

Q

N

M

Y

A

A

E

K

V

D

V

L

S

D

R

R

F

M

A

S

E

Q

A

S

F

F

L

L

E

D

R

S

C

C

K

E

T

Y

S

A

F

F

K

K

D

D

P

P

C

C

A

Q

R
|
R

V

V

G

A

R
|
R

D

D

P

P

L

L

R
|
R

K
|
K

L

L

Q

N

R

H

N

N

E

E

R
|
R

I

V

L

M

S

A

S

S

I

I

D

A

L

V

A

N

R

I

K

R

H

H

G

D

I

L

A

P

T

Y

P

K

A

N

L

L

A

L

F

K

G

G

A

A

G

A

L

L

A

G

I

Y

H

A

H

Y

A

A

L

I

binding fructose -6-phosphate: N362 (= N369), N403 (≠ S409), K508 (= K513), N513 (= N518), H516 (= H521), R587 (= R593), R590 (= R596), R594 (= R600), K595 (= K601), R601 (= R607)
binding magnesium ion: D15 (= D15), D17 (= D17), D169 (= D175)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G244 (= G250), G246 (= G252), A247 (= A253), I248 (= I254), T273 (= T279), R274 (= R280), L329 (= L335), P330 (= P336), A333 (= A339), L361 (= L368), N362 (= N369), V406 (= V412), R587 (= R593), R590 (= R596)

7ocqB Nadh bound to the dehydrogenase domain of the bifunctional mannitol-1- phosphate dehydrogenase/phosphatase mtld from acinetobacter baumannii (see paper)
52% identity, 90% coverage: 1:637/707 of query aligns to 1:635/679 of 7ocqB

query
sites
7ocqB

M

V

L

L

F

I

F

F

H

H

G

G

K

K

Q

P

I

V

F

H

S

G

A

A

I

I

F

F

D
|
D

M

M

D
|
D

G

G

T

T

L

M

F

F

D

D

T

T

E

E

R

R

L

L

R

R

F

F

R

Q

T

T

L

L

K

Q

Q

Q

A

A

S

S

L

Q

E

E

I

L

F

I

G

G

K

Q

A

E

L

F

S

S

E

H

Q

E

T

Y

L

L

I

M

G

Q

S

C

L

L

G

G

L

L

S

S

A

A

K

T

A

A

E

E

A

K

L

L

A

A

K

Q

A

R

H

-

N

-

G

-

E

-

D

-

F

L

P

Y

Y

Y

A

K

Q

E

I

I

R

R

Q

K

R

R

A

A

D

D

E

E

L

M

E

E

L

L

E

E

Y

H

V

I

R

R

N

K

H

H

G

G

V

V

P

P

I

I

K

K

A

K

G

G

L

L

L

V

E

Q

V

V

L

L

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R

R

L

L

R

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K

K

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S

G

G

L

L

T

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M

M

A

A

V

V

A

A

T

T

S

S

R

R

A

A

I

I

A

A

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E

Y

Y

L

L

I

I

N

N

A

A

N

N

V

V

L

Y

K

K

Y

F

F

F

D

D

I

V

T

I

V

T

C

C

G

G

D

D

E

E

V

V

G

E

Q

Q

G

G

K

K

P

P

H

H

P

P

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E

I

I

F

F

L

L

K

K

A

A

R

S

A

Q

L

L

N

H

C

L

E

D

P

A

G

N

Q

Q

C

C

F

L

M

M

V

F

E

E

D
|
D

S

S

E

E

N

N

G

G

M

L

L

T

S

S

A

A

M

H

R

T

A

S

E

K

G

G

Q

L

A

T

I

I

L

L

I

L

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K

D

D

I

I

K

K

P

E

P

P

A

N

P

D

E

E

I

M

K

L

A

E

G

K

A

A

L

H

K

F

A

Y

Y

Y

R

D

S

Q

M

M

H

Y

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D

F

F

L

L

A

T

D

D

L

L

S

D

E

Q

C

F

V

I

P

P

D

V

L

M

G

D

M

M

P

P

A

E

L

M

S

Q

E

E

P

P

F

F

P

P

A

Q

S

S

M

L

N

N

Q

Q

F

L

S

T

V

V

G

G

I

I

H

H

G
|
G

F
|
F

G
|
G

A
|
A

I
|
I

G

G

Y

Y

L

I

T

A

Q

Q

V

I

F

L

S

S

H

H

W

W

D

D

G

G

Y

Y

T

T

R

K

P

P

R

K

E

R

I

I

A

A

A

S

T
|
T

R
|
R

S

N

R

S

M

L

L

F

R

R

E

E

S

A

V

V

S

N

A

A

F

F

G

G

R

T

Y

Y

S

S

V

I

R

R

Y

Y

G

G

A

Q

T

F

S

S

F

Y

D

D

Q

E

T

R

I

I

D

E

N

N

V

M

R

S

M

I

I

V

D

D

M

S

D

D

D

N

D

E

D

Q

A

Q

V

M

I

L

S

E

M

M

Y

Y

T

T

T

H

A

S

E

S

I

L

I

I

G

A

L

L

S
x
C

L
|
L

P

P

E

E

Q

Q

A
|
A

I

I

R

E

S

S

Q

E

A

S

R

K

V

I

I

I

A

A

Q

K

G

G

L

L

L

Y

Q

A

R

R

F

F

E

N

R

S

R

Q

G

-

R

E

E

P

L

L

T

T

L

F

L

L

I

I

V

I

L

L

N
|
N

K

K

V

V

G

G

G

A

A

K

A

Y

F

L

V

V

R

M

R

K

H

H

V

L

-

K

Q

E

A

A

E

L

L

L

S

E

L

L

L

T

C

N

P

D

P

E

A

D

I

V

S

T

E

E

Q

H

V

I

L

L

Q

K

K

E

T

H

H

Y

F

F

A

C

E

D

T

T

V

V

V
|
V

S

N

R
|
R

I

M

V
|
V

S

S

K

K

L

L

G

S

N

N

D

Q

A

N

L

L

V

Y

R

R

Q

Q

L

L

R

R

I

I

K

K

S

H

Q

N

M

F

F

L

Q

E

N

Q

S

H

L

L

E

E

D

D

E

V

P

E

A

Q

S

E

T

I

R

E

K

D

S

C

S

N

-

K

-

L

T

T

P

P

-

D

-

Q

L

L

P

N

E

Q

Y

A

E

S

R

I

L

Y

I

V

G

D

H

N

F

M

R

R

P

R

F

N

A

F

Q

Q

P

P

S

G

S

H

A

I

M

L

S

Q

Q

S

L

M

H

D

L

L

V

I

L

L

F

F

N

H

S

S

E

E

A

T

D

D

M

M

P

P

L

I

Y

Y

A

V

E

E

R

K

G

G

S

S

D

P

L

L

E

E

R

K

L

L

R

R

Q

Q

V

V

K

V

T

L

V

V

P

D

D

Q

I

I

A

T

Q

D

I

I

Q

Q

I

L

I

I

K

K

N

N

R

R

L

L

W

W

N

N

G

G

P

V

H

H

A

A

I

M

V

L

A

A

W

W

Y

Y

A

A

S

S

L

L

L

M

G

G

H

Y

A

E

W

S

V

I

G

G

Q

V

G

A

M

M

G

G

D

D

A

H

R

L

V

V

S

K

E

A

L

F

A

A

E

E

R

N

L

L

I

I

R

-

Q

A

E

E

V

V

A

K

P

Q

A

G

L

L

V

A

A

I

E

V

Y

L

P

P

Q

N

M

Y

A

A

E

K

V

D

V

L

S

D

R

R

F

M

A

S

E

Q

A

S

F

F

L

L

E

D

R

S

C

C

K

E

T

Y

S

A

F

F

K

K

D

D

P

P

C

C

A

Q

R
|
R

V

V

G

A

R
|
R

D

D

P

P

L

L

R

R

K

K

L

L

Q

N

R

H

N

N

E

E

R

R

I

V

L

M

S

A

S

S

I

I

D

A

L

V

A

N

R

I

K

R

H

H

G

D

I

L

A

P

T

Y

P

K

A

N

L

L

A

L

F

K

G

G

A

A

G

A

L

L

A

G

I

Y

H

A

H

Y

A

A

L

I

binding magnesium ion: D15 (= D15), D17 (= D17), D170 (= D175)
binding 1,4-dihydronicotinamide adenine dinucleotide: G245 (= G250), F246 (= F251), G247 (= G252), A248 (= A253), I249 (= I254), T274 (= T279), R275 (= R280), C329 (≠ S334), L330 (= L335), A334 (= A339), N363 (= N369), V403 (= V408), R405 (= R410), V407 (= V412), R591 (= R593), R594 (= R596)

7ocsC Mannitol-1-phosphate bound to the phosphatase domain of the bifunctional mannitol-1-phosphate dehydrogenase/phosphatase mtld-d16a from acinetobacter baumannii (see paper)
50% identity, 83% coverage: 1:584/707 of query aligns to 1:564/572 of 7ocsC

query
sites
7ocsC

M

V

L

L

F

I

F

F

H

H

G

G

K

K

Q

P

I

V

F

H

S

G

A

A

I

I

F

F

D

A

M
|
M

D
|
D

G

G

T

T

L

M

F

F

D

D

T

T

E
|
E

R

R

L

L

R
|
R

F

F

R

Q

T

T

L
|
L

K

Q

Q

Q

A

A

S

S

L

Q

E

E

I

L

F

I

G

G

K

Q

A

E

L

F

S

S

E

H

Q

E

T

Y

L

L

I

M

G

Q

S
x
C

L
|
L

G
|
G

L
|
L

S

S

A

A

K

T

A

A

E

E

A

K

L

L

A

A

K

Q

A

R

H

L

N

Y

G

G

E

V

D

D

F

V

P

P

Y

Y

A

K

Q

E

I

I

R
|
R

Q

K

R

R

A

A

D

D

E

E

L

M

E

E

L

L

E

E

Y

H

V

I

R

R

N

K

H

H

G

G

V

V

P

P

I

I

K

K

A

K

G

G

L

L

L

V

E

Q

V

V

L

L

E

E

R

R

L

L

R

R

K

K

S

S

G

G

L

L

T

R

M

M

A

A

V

V

A

A

T
|
T

S
|
S

S

S

R

R

A

A

I

I

A

A

E

E

Y

Y

L

L

I

I

N

N

A

A

N

N

V

V

L

Y

K

K

Y

F

F

F

D

D

I

V

T

I

V

T

C

C

G

G

D

D

E

E

V

V

G

E

Q

Q

G

G

K
|
K

P

P

H

H

P

P

E

E

I

I

F

F

L

L

K

K

A

A

R

S

A

Q

L

L

N

H

C

L

E

D

P

A

G

N

Q

Q

C

C

F

L

M

M

V

F

E

E

D

D

S

S

E

E

N

N

G

G

M

L

L

T

S

S

A

A

M

H

R

T

A

S

E

K

G

G

Q

L

A

T

I

I

L

L

I

L

E

K

D

D

I

I

K

K

P

E

P

P

A

N

P

D

E

E

I

M

K

L

A

E

G

K

A

A

L

H

K

F

A

Y

Y

Y

R

D

S

Q

M

M

H

Y

E

D

F

F

L

L

A

T

D

D

L

L

S

D

E

Q

C

F

V

I

P

P

D

V

L

M

G

D

M

M

P

P

A

E

L

M

S

Q

E

E

P

P

F

F

P

P

A

Q

S

S

M

L

N

N

Q

Q

F

L

S

T

V

V

G

G

I

I

H

H

G

G

F

F

G

G

A

A

I

I

G

G

Y

Y

L

I

T

A

Q

Q

V

I

F

L

S

S

H

H

W

W

D

D

G

G

Y

Y

T

T

R

K

P

P

R

K

E

R

I

I

A

A

A

S

T

T

R

R

S

N

R

S

M

L

L

F

R

R

E

E

S

A

V

V

S

N

A

A

F

F

G

G

R

T

Y

Y

S

S

V

I

R

R

Y

Y

G

G

A

Q

T

F

S

S

F

Y

D

D

Q

E

T

R

I

I

D

E

N

N

V

M

R

S

M

I

I

V

D

D

M

S

D

D

D

N

D

E

D

Q

A

Q

V

M

I

L

S

E

M

M

Y

Y

T

T

T

H

A

S

E

S

I

L

I

I

G

A

L

L

S

C

L

L

P

P

E

E

Q

Q

A

A

I

I

R

E

S

S

Q

E

A

S

R

K

V

I

I

I

A

A

Q

K

G

G

L

L

L

Y

Q

A

R

R

F

F

E

N

R

S

R

Q

G

L

R

E

E

T

-

C

-

I

-

E

-

P

L

L

T

T

L

F

L

L

I

I

V

I

L

L

N

N

K

K

V

V

G

G

G

A

A

K

A

Y

F

L

V

V

R

M

R

K

H

H

V

L

-

K

Q

E

A

A

E

L

L

L

S

E

L

L

L

T

C

N

P

D

P

E

A

D

I

V

S

T

E

E

Q

H

V

I

L

L

Q

K

K

E

T

H

H

Y

F

F

A

C

E

D

T

T

V

V

S

N

R

R

I

M

V

V

S

S

K

K

L

L

G

S

N

N

D

Q

A

N

L

L

V

Y

R

R

Q

Q

L

L

R

R

I

I

K

K

S

H

Q

N

M

F

F

L

Q

E

N

Q

S

H

L

L

E

E

D

D

E

V

P

E

A

I

S

E

T

I

R

E

K

D

S

C

S

N

-

K

-

L

T

T

P

P

-

D

-

Q

L

L

P

N

E

Q

Y

A

E

S

R

I

L

Y

I

V

G

D

H

N

F

M

R

R

P

R

F

N

A

F

Q

Q

P

P

S

G

S

H

A

I

M

L

S

Q

Q

S

L

M

H

D

L

L

V

I

L

L

F

F

N

H

S

S

E

E

A

T

D

D

M

M

P

P

L

I

Y

Y

A

V

E

E

R

K

G

G

S

S

D

P

L

L

E

E

R

K

L

L

R

R

Q

Q

V

V

K

V

T

L

V

V

P

D

D

Q

I

I

A

T

Q

D

I

I

Q

Q

I

L

I

I

K

K

N

N

R

R

L

L

W

W

N

N

G

G

P

V

H

H

A

A

I

M

V

L

A

A

W

-

Y

-

A

-

S

-

L

-

G

-

H

-

A

A

W

E

V

V

G

K

Q

Q

G

G

M

L

G

-

D

-

A

-

R

-

V

-

S

-

E

-

L

-

A

-

E

-

R

-

L

-

I

-

R

-

Q

-

E

-

V

-

A

-

P

-

A

A

L

I

V

V

A

L

E

-

Y

-

P

P

Q

N

M

Y

A

A

E

K

V

D

V

L

S

D

R

R

F

M

A

S

E

Q

A

S

F

F

L

L

E

D

R

S

C

C

K

E

binding D-Mannitol-1-phosphate: M16 (= M16), D17 (= D17), E24 (= E24), R27 (= R27), L31 (= L31), C51 (≠ S51), L52 (= L52), G53 (= G53), L54 (= L54), R77 (= R77), T117 (= T117), S118 (= S118), K150 (= K150)

P77247 Hexitol phosphatase B; 2-deoxyglucose-6-phosphate phosphatase; Mannitol-1-phosphatase; Sorbitol-6-phosphatase; Sugar-phosphatase; EC 3.1.3.68; EC 3.1.3.22; EC 3.1.3.50; EC 3.1.3.23 from Escherichia coli (strain K12) (see paper)
30% identity, 28% coverage: 7:202/707 of query aligns to 5:195/222 of P77247

query
sites
P77247

K

R

Q

Q

I

I

F

L

S

A

A

A

I

I

F

F

D
|
D

M

M

D
|
D

G

G

T

L

L

L

F

I

D

D

T

S

E

E

R

P

L

L

R

W

F

D

R

R

T

-

L

-

K

-

Q

-

A

A

S

E

L

L

E

D

I

V

-

M

-

A

-

S

F

L

G

G

K

V

A

D

L

I

S

S

E

R

Q

R

T

N

-

E

L

L

I

P

G

D

S

T

L

L

G

G

L

L

S

R

A

I

K

D

K

M

A

V

E

V

A

D

L

L

A

W

K

Y

A

A

H

R

N

Q

G

P

E

W

D

N

F

G

P

P

Y

S

A

R

Q

Q

-

E

I

V

R

V

Q

E

R

R

A

V

D

I

E

A

L

R

E

A

L

I

E

S

Y

L

V

V

R

E

N

E

H

T

G

R

V

-

P

P

I

L

K

L

A

P

G

G

L

V

L

R

E

E

V

A

L

V

E

A

R

L

L

C

R

K

K

E

S

Q

G

G

L

L

T

L

M

V

A

G

V

L

A

A

T

S

S

A

S

S

R

P

R

L

A

H

I

M

A

L

E

E

E

K

Y

V

L

L

I

T

N

M

A

F

N

D

V

L

L

R

K

D

Y

S

F

F

D

D

I

A

T

L

V

A

C

S

G

A

D

E

E

K

V

L

G

P

Q

Y

G

S

K

K

P

P

H

H

P

P

E

Q

I

V

F

Y

L

L

K

D

A

C

A

A

R

A

A

K

L

L

N

G

C

V

E

D

P

P

G

L

Q

T

C

C

F

V

M

A

V

L

E

E

D
|
D

S

S

E

V

N

N

G

G

M

M

L

I

S

A

A

S

M

K

R

A

A

A

E

R

G

M

Q

R

A

S

I

I

L

V

I

V

E

-

D

-

I

-

K

-

P

-

P

P

A

A

P

P

E

E

D13 (= D15) binding ; mutation to A: Loss of phosphatase activity.
D15 (= D17) binding
D173 (= D175) binding

1te2A Putative phosphatase ynic from escherichia coli k12
30% identity, 28% coverage: 7:202/707 of query aligns to 1:191/218 of 1te2A

query
sites
1te2A

K

R

Q

Q

I

I

F

L

S

A

A

A

I

I

F

F

D
|
D

M
|
M

D
|
D

G

G

T

L

L

L

F

I

D

D

T
x
S

E

E

R

P

L

L

R

W

F

D

R

R

T

-

L

-

K

-

Q

-

A

A

S

E

L

L

E

D

I

V

-

M

-

A

-

S

F

L

G

G

K

V

A

D

L

I

S

S

E

R

Q

R

T

N

-

E

L

L

I

P

G
x
D

S

T

L

L

G
|
G

L

L

S

R

A

I

K

D

K

M

A

V

E

V

A

D

L

L

A

W

K

Y

A

A

H

R

N

Q

G

P

E

W

D

N

F

G

P

P

Y

S

A

R

Q

Q

-

E

I

V

R

V

Q

E

R

R

A

V

D

I

E

A

L

R

E

A

L

I

E

S

Y

L

V

V

R

E

N

E

H

T

G

R

V

-

P

P

I

L

K

L

A

P

G

G

L

V

L

R

E

E

V

A

L

V

E

A

R

L

L

C

R

K

K

E

S

Q

G

G

L

L

T

L

M

V

A

G

V

L

A

A

T
x
S

S
x
A

S

S

R

P

R

L

A

H

I

M

A

L

E

E

E

K

Y

V

L

L

I

T

N

M

A

F

N

D

V

L

L

R

K

D

Y

S

F

F

D

D

I

A

T

L

V

A

C

S

G

A

D

E

E

K

V

L

G

P

Q

Y

G

S

K
|
K

P

P

H

H

P

P

E

Q

I

V

F

Y

L

L

K

D

A

C

A

A

R

A

A

K

L

L

N

G

C

V

E

D

P

P

G

L

Q

T

C

C

F

V

M

A

V

L

E
|
E

D
|
D

S

S

E

V

N

N

G

G

M

M

L

I

S

A

A

S

M

K

R

A

A

A

E

R

G

M

Q

R

A

S

I

I

L

V

I

V

E

-

D

-

I

-

K

-

P

-

P

P

A

A

P

P

E

E

active site: D9 (= D15), D11 (= D17), S17 (≠ T23), D44 (≠ G50), S111 (≠ T117), A112 (≠ S118), K144 (= K150), E168 (= E174), D169 (= D175)
binding calcium ion: D9 (= D15), D11 (= D17), D169 (= D175)
binding 2-phosphoglycolic acid: D9 (= D15), M10 (= M16), D11 (= D17), G47 (= G53), S111 (≠ T117), A112 (≠ S118), K144 (= K150)

5olwA 5-fluorotryptophan labeled beta-phosphoglucomutase in an open conformation (see paper)
28% identity, 24% coverage: 9:180/707 of query aligns to 1:175/224 of 5olwA

query
sites
5olwA

I

M

F

F

S

K

A

A

I

V

-

L

F

F

D
|
D

M
x
L

D
|
D

G

G

T

V

L

I

F

T

D

D

T
|
T

E

A

R

E

L

Y

R

H

F

F

R

R

T

A

L

W

K

K

Q

A

A

L

S

A

L

E

E

E

I

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

-

Q

F

F

G

N

K

E

A

Q

L

L

S
x
K

-

G

-

V

-

S

-

R

E

E

Q

D

T

S

L

L

I

Q

G

K

S

I

L

L

G

D

L

L

S

A

A

D

K

K

A

V

E

S

A

A

L

-

A

-

K

-

A

-

H

-

N

-

G

-

E

E

D

E

F

F

P

-

Y

-

A

K

Q

E

I

L

R

A

Q

K

R

R

A

K

D

N

E

D

L

N

E

Y

L

V

E

K

Y

M

V

I

R

Q

N

D

H

V

G

S

-
x
P

V

A

P

D

I
x
V

K

Y

A

P

G

G

L

I

L

L

E

Q

V

L

L

L

E

K

R

D

L

L

R

R

K

S

S

N

G

K

L

I

T

K

M

I

A

A

V

L

A

A

T
x
S

S
x
A

S

S

R

K

R

N

A

G

I

-

A

-

E

-

E

P

Y

F

L

L

I

L

N
x
E

-

R

A

M

N
|
N

V

L

L

T

K

G

Y

Y

F

F

D

D

I

A

T

I

V

A

C

D

G

P

D

A

E

E

V

V

G

A

Q

A

G

S

K
|
K

P

P

H

A

P

P

E

D

I

I

F

F

L

I

K

A

A

A

R

H

A

A

L

V

N

G

C

V

E

A

P

P

G

S

Q

E

C

S

F

I

M

G

V

L

E
|
E

D
|
D

S
|
S

E

Q

N

A

G

G

M

I

active site: D8 (= D15), L9 (≠ M16), D10 (= D17), T16 (= T23), K45 (≠ S44), S114 (≠ T117), A115 (≠ S118), K145 (= K150), E169 (= E174), D170 (= D175)
binding calcium ion: D8 (= D15), D10 (= D17), P89 (vs. gap), V92 (≠ I95), E124 (≠ N130), N127 (= N132), E169 (= E174), D170 (= D175), S171 (= S176)

4c4sA Structure of beta-phosphoglucomutase in complex with an alpha- fluorophosphonate analogue of beta-glucose-1-phosphate and magnesium trifluoride (see paper)
30% identity, 24% coverage: 9:180/707 of query aligns to 1:172/215 of 4c4sA

query
sites
4c4sA

I

M

F

F

S

K

A

A

I

V

-

L

F

F

D
|
D

M
x
L

D
|
D

G

G

T

V

L

I

F

T

D

D

T
|
T

E

A

R

E

L

Y

R
x
H

F

F

R

R

T

A

L
x
W

K

K

Q

A

A

L

S

A

L

E

E

E

I

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

-

Q

F

F

G

N

K

E

A

Q

L
|
L

S
x
K

-
x
G

-
x
V

-

S

-
x
R

E

E

Q

D

T

S

L

L

-

Q

-

K

I

I

G

L

S

D

L

L

G

K

L

V

S

S

A

A

K

E

A

F

E

K

A

E

L

L

A

A

K

K

A

R

H

K

N

N

G

D

E

N

D

Y

F

V

P

K

Y

M

A

I

Q

Q

I

D

R

V

Q

S

R

P

A

A

D

D

E

-

L

-

E

-

L

-

E

-

Y

-

V

-

R

-

N

-

H

-

G

-

V

-

P

-

I

V

K

Y

A

P

G

G

L

I

L

L

E

Q

V

L

L

L

E

K

R

D

L

L

R

R

K

S

S

N

G

K

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I

T

K

M

I

A

A

V

L

A

A

T
x
S

S
x
A

S
|
S

R

K

R

N

A

G

I

-

A

-

E

-

E

P

Y

F

L

L

I

L

N

E

-

R

A

M

N

N

V

L

L

T

K

G

Y

Y

F

F

D

D

I

A

T

I

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A

C

D

G

P

D

A

E

E

V

V

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A

Q

A

G

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K

P

P

H

A

P

P

E

D

I

I

F

F

L

I

K

A

A

A

R

H

A

A

L

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N

G

C

V

E

A

P

P

G

S

Q

E

C

S

F

I

M

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L

E
|
E

D
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D

S

S

E

Q

N

A

G

G

M

I

active site: D8 (= D15), L9 (≠ M16), D10 (= D17), T16 (= T23), K45 (≠ S44), S111 (≠ T117), A112 (≠ S118), K142 (= K150), E166 (= E174), D167 (= D175)
binding (1R)-1,5-anhydro-1-[(S)-fluoro(phosphono)methyl]-D-glucitol: D10 (= D17), H20 (≠ R27), W24 (≠ L31), L44 (= L43), G46 (vs. gap), V47 (vs. gap), R49 (vs. gap), S113 (= S119)
binding magnesium ion: D8 (= D15), D10 (= D17), D167 (= D175)
binding trifluoromagnesate: D8 (= D15), L9 (≠ M16), D10 (= D17), S111 (≠ T117), A112 (≠ S118), K142 (= K150)

Q8VZ10 Protein SUPPRESSOR OF QUENCHING 1, chloroplastic; EC 3.1.3.- from Arabidopsis thaliana (Mouse-ear cress) (see paper)
28% identity, 26% coverage: 8:194/707 of query aligns to 73:261/1055 of Q8VZ10

query
sites
Q8VZ10

Q

K

I

V

F

S

S

A

A

V

I

L

F

F

D
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D

M

M

D

D

G

G

T

V

L

L

F

C

D

N

T

S

E

E

R

D

L

L

R

S

F

R

R

R

T

-

L

-

K

-

Q

-

A

A

S

A

L

V

E

D

I

V

F

F

-

T

-

E

-

M

G

G

K

V

A

E

L

V

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T

E

V

Q

D

T

D

L

F

I

V

G

P

S

F

L

M

G

G

L

T

S

G

-

E

A

A

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K

K

F

A

L

E

G

A

G

L

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A

A

K

S

A

V

H

K

N

E

G

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E

K

D

G

F

F

P

D

Y

P

A

D

Q

A

I

A

R

K

Q

E

R

R

A

F

D

F

E

E

L

I

E

Y

L

L

E

D

-

K

Y

Y

V

A

R

K

N

P

H

E

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S

V

G

P

I

I

G

K

F

A

P

G

G

L

A

L

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E

E

V

L

L

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T

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E

L

C

R

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K

N

S

K

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G

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L

T

K

M

V

A

A

V

V

A

A

T

S

S

S

S

A

R

D

R

R

A

I

I

K

A

V

E

D

E

A

Y

N

L

L

I

K

N

A

A

A

N

G

V

L

-

S

L

L

K

T

Y

M

F

F

D

D

I

A

T

I

V

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C

S

G

A

D

D

E

A

V

F

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E

Q

N

G

L

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K

P

P

H

A

P

P

E

D

I

I

F

F

L

L

K

A

A

A

R

K

A

I

L

L

N

G

C

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E

P

P

T

G

S

Q

E

C

C

F

V

M

V

V

I

E

E

D

D

S

A

E

L

N

A

G

G

M

V

L

Q

S

A

A

A

M

Q

R

A

A

A

E

N

G

M

Q

R

A

C

I

I

L

A

I

V

E

K

D80 (= D15) mutation to N: Complete rescue in complementation test of the nonphotochemical quenching (NPQ) phenotype observed in disrupted plants.

Sites not aligning to the query:

431:434 CINC→SINS: No rescue in complementation test of the nonphotochemical quenching (NPQ) phenotype observed in disrupted plants.
859 E→K: In soq1-2; high light intensity-dependent and irreversible nonphotochemical quenching (NPQ) due to a decrease in chlorophyll excited-state lifetime.

P71447 Beta-phosphoglucomutase; Beta-PGM; EC 5.4.2.6 from Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) (see 3 papers)
28% identity, 24% coverage: 9:180/707 of query aligns to 1:175/221 of P71447

query
sites
P71447

I

M

F

F

S

K

A

A

I

V

-

L

F

F

D
|
D

M

L

D
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D

G

G

T

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I

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D

T
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T

E

A

R

E

L

Y

R
x
H

F

F

R

R

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A

L

W

K

K

Q

A

A

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S

A

L

E

E

E

I

I

-

G

-

I

-

N

-

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F

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L

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x
K

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x
G

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x
R

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x
S

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L

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L

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A

A

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K

K

A

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S

A

A

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-

A

-

K

-

A

-

H

-

N

-

G

-

E

E

D

E

F

F

P

-

Y

-

A

K

Q

E

I

L

R

A

Q

K

R

R

A
x
K

D

N

E

D

L

N

E

Y

L

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Y

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N

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D

L

L

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R

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S

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L

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T

K

M

I

A

A

V

L

A

A

T

S

S

A

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S

R

K

R

N

A

G

I

-

A

-

E

-

E

P

Y

F

L

L

I

L

N

E

A

K

-

M

N

N

V

L

L

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Y

Y

F

F

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D

I

A

T

I

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A

C

D

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D

A

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E

V

V

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A

Q

A

G

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K

K

P

P

H

A

P

P

E

D

I

I

F

F

L

I

K

A

A

A

R

H

A

A

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N

G

C

V

E

A

P

P

G

S

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E

C

S

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V

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E

D
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D

S

S

E

Q

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A

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I

D8 (= D15) modified: 4-aspartylphosphate; mutation to A: Inactive.; mutation to E: Inactive.
D10 (= D17) mutation to A: Inactive.; mutation to E: Inactive.; mutation to N: Inactive.; mutation to S: Inactive.
T16 (= T23) mutation to P: 500-fold reduction in the rate constant for Asp-8 phosphorylation by beta-G1,6bisP. 6,700-fold reduction in the apparent rate constant for cycling of the phosphorylated enzyme to convert beta-G1P to G6P. 13-fold increase in the estimated rate constant for phosphoryl transfer from the phospho-Asp8 to water.
H20 (≠ R27) mutation to A: Impairs Asp-8 phosphorylation by beta-G1,6bisP and phosphoryl transfer from the phospho-Asp8 to the substrate beta-G1P.; mutation to N: 300-fold reduction in the conversion of beta-G1P to G6P in the presence of beta-G1,6bisP.; mutation to Q: 8-fold reduction in the conversion of beta-G1P to G6P in the presence of beta-G1,6bisP.
K45 (≠ S44) mutation to A: 20'000-fold decrease in kcat/KM.
G46 (vs. gap) mutation to A: 1'000'000-fold decrease in kcat/KM.; mutation to P: 100'000-fold decrease in kcat/KM.; mutation to V: 10'000-fold decrease in kcat/KM.
R49 (vs. gap) mutation to K: 1'000'000-fold decrease in kcat/KM.
S52 (≠ T47) mutation to A: Wild-type activity.
K76 (≠ A80) mutation to A: 100-fold reduction in the conversion of beta-G1P to G6P in the presence of beta-G1,6bisP.
D170 (= D175) mutation to A: Impaired, but active with an increase in the affinity for G1P.

6h91A Phosphorylated beta-phosphoglucomutase from lactococcus lactis in an open conformer to 2.4 a
28% identity, 24% coverage: 9:180/707 of query aligns to 1:175/218 of 6h91A

query
sites
6h91A

I

M

F

F

S

K

A

A

I

V

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L

F

F

D
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D

M

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D
|
D

G

G

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I

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D

T

T

E

A

R

E

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Y

R

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F

F

R

R

T

A

L

W

K

K

Q

A

A

L

S

A

L

E

E

E

I

I

-

G

-

I

-

N

-

G

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V

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D

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Q

F

F

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K

E

A

Q

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S

K

-

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S

-
x
R

E

E

Q

D

T

S

L

L

I

Q

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K

S

I

L

L

G

D

L

L

S

A

A

D

K

K

A

V

E

S

A

A

L

-

A

-

K

-

A

-

H

-

N

-

G

-

E

E

D

E

F

F

P

-

Y

-

A

K

Q

E

I

L

R

A

Q

K

R

R

A

K

D

N

E

D

L

N

E

Y

L

V

E

K

Y

M

V

I

R

Q

N

D

H

V

G

S

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P

V

A

P

D

I

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K

Y

A

P

G

G

L

I

L

L

E

Q

V

L

L

L

E

K

R

D

L

L

R

R

K

S

S

N

G

K

L

I

T

K

M

I

A

A

V

L

A

A

T

S

S

A

S

S

R
x
K

R

N

A

G

I

-

A

-

E

-

E

P

Y

F

L

L

I

L

N

E

-

R

A

M

N

N

V

L

L

T

K

G

Y

Y

F

F

D

D

I

A

T

I

V

A

C

D

G

P

D

A

E

E

V

V

G

A

Q

A

G

S

K

K

P

P

H

A

P

P

E

D

I

I

F

F

L

I

K

A

A

A

R

H

A

A

L

V

N

G

C

V

E

A

P

P

G

S

Q

E

C

S

F

I

M

G

V

L

E

E

D
|
D

S

S

E

Q

N

A

G

G

M

I

binding magnesium ion: D8 (= D15), D10 (= D17), D170 (= D175)
binding acetylphosphate: R49 (vs. gap), K117 (≠ R120)

4c4rA Structure of beta-phosphoglucomutase in complex with a phosphonate analogue of beta-glucose-1-phosphate and magnesium trifluoride (see paper)
28% identity, 24% coverage: 9:180/707 of query aligns to 1:175/218 of 4c4rA

query
sites
4c4rA

I

M

F

F

S

K

A

A

I

V

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L

F

F

D
|
D

M
x
L

D
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D

G

G

T

V

L

I

F

T

D

D

T
|
T

E

A

R

E

L

Y

R
x
H

F

F

R

R

T

A

L
x
W

K

K

Q

A

A

L

S

A

L

E

E

E

I

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

-

Q

F

F

G

N

K

E

A

Q

L
|
L

S
x
K

-
x
G

-
x
V

-

S

-
x
R

E

E

Q

D

T
x
S

L

L

I

Q

G

K

S

I

L

L

G

D

L

L

S

A

A

D

K

K

A

V

E

S

A

A

L

-

A

-

K

-

A

-

H

-

N

-

G

-

E

E

D

E

F

F

P

-

Y

-

A

K

Q

E

I

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R

A

Q

K

R

R

A

K

D

N

E

D

L

N

E

Y

L

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E

K

Y

M

V

I

R

Q

N

D

H

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S

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P

V

A

P

D

I

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K

Y

A

P

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G

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I

L

L

E

Q

V

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L

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D

L

L

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R

K

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S

N

G

K

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T

K

M

I

A

A

V

L

A

A

T
x
S

S
x
A

S
|
S

R
x
K

R

N

A

G

I

-

A

-

E

-

E

P

Y

F

L

L

I

L

N

E

-

R

A

M

N

N

V

L

L

T

K

G

Y

Y

F

F

D

D

I

A

T

I

V

A

C

D

G

P

D

A

E

E

V

V

G

A

Q

A

G

S

K
|
K

P

P

H

A

P

P

E

D

I

I

F

F

L

I

K

A

A

A

R

H

A

A

L

V

N

G

C

V

E

A

P

P

G

S

Q

E

C

S

F

I

M

G

V

L

E
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E

D
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D

S

S

E

Q

N

A

G

G

M

I

active site: D8 (= D15), L9 (≠ M16), D10 (= D17), T16 (= T23), K45 (≠ S44), S114 (≠ T117), A115 (≠ S118), K145 (= K150), E169 (= E174), D170 (= D175)
binding magnesium ion: D8 (= D15), D10 (= D17), D170 (= D175)
binding trifluoromagnesate: D8 (= D15), L9 (≠ M16), D10 (= D17), S114 (≠ T117), A115 (≠ S118), K145 (= K150)
binding (1R)-1,5-anhydro-1-(phosphonomethyl)-D-glucitol: D10 (= D17), H20 (≠ R27), W24 (≠ L31), L44 (= L43), G46 (vs. gap), V47 (vs. gap), R49 (vs. gap), S52 (≠ T47), S116 (= S119), K117 (≠ R120)

3zi4A The structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate and scandium tetrafluoride
28% identity, 24% coverage: 9:180/707 of query aligns to 1:175/218 of 3zi4A

query
sites
3zi4A

I

M

F

F

S

K

A

A

I

V

-

L

F

F

D
|
D

M
x
L

D
|
D

G

G

T

V

L

I

F

T

D

D

T
|
T

E

A

R

E

L

Y

R
x
H

F

F

R

R

T

A

L

W

K

K

Q

A

A

L

S

A

L

E

E

E

I

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

-

Q

F

F

G

N

K

E

A

Q

L

L

S
x
K

-
x
G

-
x
V

-

S

-
x
R

E

E

Q

D

T

S

L

L

I

Q

G

K

S

I

L

L

G

D

L

L

S

A

A

D

K

K

A

V

E

S

A

A

L

-

A

-

K

-

A

-

H

-

N

-

G

-

E

E

D

E

F

F

P

-

Y

-

A

K

Q

E

I

L

R

A

Q

K

R

R

A

K

D

N

E

D

L

N

E

Y

L

V

E

K

Y

M

V

I

R

Q

N

D

H

V

G

S

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P

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A

P

D

I

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Y

A

P

G

G

L

I

L

L

E

Q

V

L

L

L

E

K

R

D

L

L

R

R

K

S

S

N

G

K

L

I

T

K

M

I

A

A

V

L

A

A

T
x
S

S
x
A

S
|
S

R
x
K

R

N

A

G

I

-

A

-

E

-

E

P

Y

F

L

L

I

L

N

E

-

R

A

M

N

N

V

L

L

T

K

G

Y

Y

F

F

D

D

I

A

T

I

V

A

C

D

G

P

D

A

E

E

V

V

G

A

Q

A

G

S

K
|
K

P

P

H

A

P

P

E

D

I

I

F

F

L

I

K

A

A

A

R

H

A

A

L

V

N

G

C

V

E

A

P

P

G

S

Q

E

C

S

F

I

M

G

V

L

E
|
E

D
|
D

S

S

E

Q

N

A

G

G

M

I

active site: D8 (= D15), L9 (≠ M16), D10 (= D17), T16 (= T23), K45 (≠ S44), S114 (≠ T117), A115 (≠ S118), K145 (= K150), E169 (= E174), D170 (= D175)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D17), H20 (≠ R27), G46 (vs. gap), V47 (vs. gap), R49 (vs. gap), S116 (= S119), K117 (≠ R120)
binding magnesium ion: D8 (= D15), D10 (= D17), D170 (= D175)
binding Scandium Tetrafluoride: D8 (= D15), L9 (≠ M16), D10 (= D17), S114 (≠ T117), A115 (≠ S118), K145 (= K150)

2wf8A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate, glucose-1-phosphate and beryllium trifluoride (see paper)
28% identity, 24% coverage: 9:180/707 of query aligns to 1:175/218 of 2wf8A

query
sites
2wf8A

I

M

F

F

S

K

A

A

I

V

-

L

F

F

D
|
D

M
x
L

D
|
D

G

G

T

V

L

I

F

T

D

D

T
|
T

E

A

R

E

L

Y

R
x
H

F

F

R

R

T

A

L
x
W

K

K

Q

A

A

L

S

A

L

E

E

E

I

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

-

Q

F

F

G

N

K

E

A

Q

L
|
L

S
x
K

-
x
G

-
x
V

-

S

-
x
R

E

E

Q

D

T
x
S

L

L

I

Q

G

K

S

I

L

L

G

D

L

L

S

A

A

D

K

K

A

V

E

S

A

A

L

-

A

-

K

-

A

-

H

-

N

-

G

-

E

E

D

E

F

F

P

-

Y

-

A

K

Q

E

I

L

R

A

Q

K

R

R

A

K

D

N

E

D

L

N

E

Y

L

V

E

K

Y

M

V

I

R

Q

N

D

H

V

G

S

-

P

V

A

P

D

I

V

K

Y

A

P

G

G

L

I

L

L

E

Q

V

L

L

L

E

K

R

D

L

L

R

R

K

S

S

N

G

K

L

I

T

K

M

I

A

A

V

L

A

A

T
x
S

S
x
A

S
|
S

R
x
K

R

N

A

G

I

-

A

-

E

-

E

P

Y

F

L

L

I

L

N

E

-

R

A

M

N

N

V

L

L

T

K

G

Y

Y

F

F

D

D

I

A

T

I

V

A

C

D

G

P

D

A

E

E

V

V

G

A

Q

A

G

S

K
|
K

P

P

H

A

P

P

E

D

I

I

F

F

L

I

K

A

A

A

R

H

A

A

L

V

N

G

C

V

E

A

P

P

G

S

Q

E

C

S

F

I

M

G

V

L

E
|
E

D
|
D

S

S

E

Q

N

A

G

G

M

I

active site: D8 (= D15), L9 (≠ M16), D10 (= D17), T16 (= T23), K45 (≠ S44), S114 (≠ T117), A115 (≠ S118), K145 (= K150), E169 (= E174), D170 (= D175)
binding beryllium trifluoride ion: D8 (= D15), L9 (≠ M16), D10 (= D17), S114 (≠ T117), A115 (≠ S118), K145 (= K150)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D17), H20 (≠ R27), G46 (vs. gap), V47 (vs. gap), R49 (vs. gap), A115 (≠ S118), S116 (= S119), K117 (≠ R120)
binding 1-O-phosphono-alpha-D-glucopyranose: D10 (= D17), H20 (≠ R27), W24 (≠ L31), L44 (= L43), G46 (vs. gap), V47 (vs. gap), R49 (vs. gap), S52 (≠ T47), A115 (≠ S118), S116 (= S119), K117 (≠ R120)
binding magnesium ion: D8 (= D15), D10 (= D17), D170 (= D175)

2wf7A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphonate and aluminium tetrafluoride (see paper)
28% identity, 24% coverage: 9:180/707 of query aligns to 1:175/218 of 2wf7A

query
sites
2wf7A

I

M

F

F

S

K

A

A

I

V

-

L

F

F

D
|
D

M
x
L

D
|
D

G

G

T

V

L

I

F

T

D

D

T
|
T

E

A

R

E

L

Y

R

H

F

F

R

R

T

A

L

W

K

K

Q

A

A

L

S

A

L

E

E

E

I

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

-

Q

F

F

G

N

K

E

A

Q

L

L

S
x
K

-
x
G

-
x
V

-

S

-
x
R

E

E

Q

D

T

S

L

L

I

Q

G

K

S

I

L

L

G

D

L

L

S

A

A

D

K

K

A

V

E

S

A

A

L

-

A

-

K

-

A

-

H

-

N

-

G

-

E

E

D

E

F

F

P

-

Y

-

A

K

Q

E

I

L

R

A

Q

K

R

R

A

K

D

N

E

D

L

N

E

Y

L

V

E

K

Y

M

V

I

R

Q

N

D

H

V

G

S

-

P

V

A

P

D

I

V

K

Y

A

P

G

G

L

I

L

L

E

Q

V

L

L

L

E

K

R

D

L

L

R

R

K

S

S

N

G

K

L

I

T

K

M

I

A

A

V

L

A

A

T
x
S

S
x
A

S
|
S

R
x
K

R
x
N

A

G

I

-

A

-

E

-

E

P

Y

F

L

L

I

L

N

E

-

R

A

M

N

N

V

L

L

T

K

G

Y

Y

F

F

D

D

I

A

T

I

V

A

C

D

G

P

D

A

E

E

V

V

G

A

Q

A

G

S

K
|
K

P

P

H

A

P

P

E

D

I

I

F

F

L

I

K

A

A

A

R

H

A

A

L

V

N

G

C

V

E

A

P

P

G

S

Q

E

C

S

F

I

M

G

V

L

E
|
E

D
|
D

S

S

E

Q

N

A

G

G

M

I

active site: D8 (= D15), L9 (≠ M16), D10 (= D17), T16 (= T23), K45 (≠ S44), S114 (≠ T117), A115 (≠ S118), K145 (= K150), E169 (= E174), D170 (= D175)
binding tetrafluoroaluminate ion: D8 (= D15), L9 (≠ M16), D10 (= D17), S114 (≠ T117), K145 (= K150)
binding 6,7-dideoxy-7-phosphono-beta-D-gluco-heptopyranose: D10 (= D17), G46 (vs. gap), V47 (vs. gap), R49 (vs. gap), S116 (= S119), K117 (≠ R120), N118 (≠ R121)
binding magnesium ion: D8 (= D15), D10 (= D17), D170 (= D175)

2wf6A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate and aluminium tetrafluoride (see paper)
28% identity, 24% coverage: 9:180/707 of query aligns to 1:175/218 of 2wf6A

query
sites
2wf6A

I

M

F

F

S

K

A

A

I

V

-

L

F

F

D
|
D

M
x
L

D
|
D

G

G

T

V

L

I

F

T

D

D

T
|
T

E

A

R

E

L

Y

R

H

F

F

R

R

T

A

L

W

K

K

Q

A

A

L

S

A

L

E

E

E

I

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

-

Q

F

F

G

N

K

E

A

Q

L

L

S
x
K

-
x
G

-
x
V

-

S

-
x
R

E

E

Q

D

T

S

L

L

I

Q

G

K

S

I

L

L

G

D

L

L

S

A

A

D

K

K

A

V

E

S

A

A

L

-

A

-

K

-

A

-

H

-

N

-

G

-

E

E

D

E

F

F

P

-

Y

-

A

K

Q

E

I

L

R

A

Q

K

R

R

A

K

D

N

E

D

L

N

E

Y

L

V

E

K

Y

M

V

I

R

Q

N

D

H

V

G

S

-

P

V

A

P

D

I

V

K

Y

A

P

G

G

L

I

L

L

E

Q

V

L

L

L

E

K

R

D

L

L

R

R

K

S

S

N

G

K

L

I

T

K

M

I

A

A

V

L

A

A

T
x
S

S
x
A

S
|
S

R
x
K

R

N

A

G

I

-

A

-

E

-

E

P

Y

F

L

L

I

L

N

E

-

R

A

M

N

N

V

L

L

T

K

G

Y

Y

F

F

D

D

I

A

T

I

V

A

C

D

G

P

D

A

E

E

V

V

G

A

Q

A

G

S

K
|
K

P

P

H

A

P

P

E

D

I

I

F

F

L

I

K

A

A

A

R

H

A

A

L

V

N

G

C

V

E

A

P

P

G

S

Q

E

C

S

F

I

M

G

V

L

E
|
E

D
|
D

S

S

E

Q

N

A

G

G

M

I

active site: D8 (= D15), L9 (≠ M16), D10 (= D17), T16 (= T23), K45 (≠ S44), S114 (≠ T117), A115 (≠ S118), K145 (= K150), E169 (= E174), D170 (= D175)
binding tetrafluoroaluminate ion: D8 (= D15), L9 (≠ M16), D10 (= D17), S114 (≠ T117), K145 (= K150)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D17), G46 (vs. gap), V47 (vs. gap), R49 (vs. gap), S116 (= S119), K117 (≠ R120)
binding magnesium ion: D8 (= D15), D10 (= D17), D170 (= D175)

2wf5A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate and trifluoromagnesate (see paper)
28% identity, 24% coverage: 9:180/707 of query aligns to 1:175/218 of 2wf5A

query
sites
2wf5A

I

M

F

F

S

K

A

A

I

V

-

L

F

F

D
|
D

M
x
L

D
|
D

G

G

T

V

L

I

F

T

D

D

T
|
T

E

A

R

E

L

Y

R
x
H

F

F

R

R

T

A

L

W

K

K

Q

A

A

L

S

A

L

E

E

E

I

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

-

Q

F

F

G

N

K

E

A

Q

L

L

S
x
K

-
x
G

-
x
V

-

S

-
x
R

E

E

Q

D

T

S

L

L

I

Q

G

K

S

I

L

L

G

D

L

L

S

A

A

D

K

K

A

V

E

S

A

A

L

-

A

-

K

-

A

-

H

-

N

-

G

-

E

E

D

E

F

F

P

-

Y

-

A

K

Q

E

I

L

R

A

Q

K

R

R

A

K

D

N

E

D

L

N

E

Y

L

V

E

K

Y

M

V

I

R

Q

N

D

H

V

G

S

-

P

V

A

P

D

I

V

K

Y

A

P

G

G

L

I

L

L

E

Q

V

L

L

L

E

K

R

D

L

L

R

R

K

S

S

N

G

K

L

I

T

K

M

I

A

A

V

L

A

A

T
x
S

S
x
A

S
|
S

R

K

R

N

A

G

I

-

A

-

E

-

E

P

Y

F

L

L

I

L

N

E

-

R

A

M

N

N

V

L

L

T

K

G

Y

Y

F

F

D

D

I

A

T

I

V

A

C

D

G

P

D

A

E

E

V

V

G

A

Q

A

G

S

K
|
K

P

P

H

A

P

P

E

D

I

I

F

F

L

I

K

A

A

A

R

H

A

A

L

V

N

G

C

V

E

A

P

P

G

S

Q

E

C

S

F

I

M

G

V

L

E
|
E

D
|
D

S

S

E

Q

N

A

G

G

M

I

active site: D8 (= D15), L9 (≠ M16), D10 (= D17), T16 (= T23), K45 (≠ S44), S114 (≠ T117), A115 (≠ S118), K145 (= K150), E169 (= E174), D170 (= D175)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D17), H20 (≠ R27), G46 (vs. gap), V47 (vs. gap), R49 (vs. gap), A115 (≠ S118), S116 (= S119)
binding magnesium ion: D8 (= D15), D10 (= D17), D170 (= D175)
binding trifluoromagnesate: D8 (= D15), L9 (≠ M16), D10 (= D17), S114 (≠ T117), A115 (≠ S118), K145 (= K150)

Query Sequence

>PP_2052 FitnessBrowser__Putida:PP_2052
MLFFHGKQIFSAIFDMDGTLFDTERLRFRTLKQASLEIFGKALSEQTLIGSLGLSAKKAE
ALAKAHNGEDFPYAQIRQRADELELEYVRNHGVPIKAGLLEVLERLRKSGLTMAVATSSR
RAIAEEYLINANVLKYFDITVCGDEVGQGKPHPEIFLKAARALNCEPGQCFMVEDSENGM
LSAMRAEGQAILIEDIKPPAPEIKAGALKAYRSMHEFLADLSECVPDLGMPALSEPFPAS
MNQFSVGIHGFGAIGGGYLTQVFSHWDGYTRPREIIAATRSRMLRESVSAFGRYSVRYGA
TSFDQTIDNVRMIDMDDDDAVISMYTTAEIIGLSLPEQAIRSQARVIAQGLLQRFERRGR
ELTLLIVLNKVGGAAFVRRHVQAELSLLCPPAISEQVLQKTHFAETVVSRIVSKLGNDAL
VRQLRIKSQMFQNSLEDEPASTRKSSTPLPEYERLIGHFRPFAQPSSAMSQLHLVLFNSE
ADMPLYAERGSDLLERLRQVKTVPDIAQIQIIKNRLWNGPHAIVAWYASLLGHAWVGQGM
GDARVSELAERLIRQEVAPALVAEYPQMAEVVSRFAEAFLERCKTSFKDPCARVGRDPLR
KLQRNERILSSIDLARKHGIATPALAFGAGLAIHHALNCSNSKDLESQAIRQVYLDNQES
VEAVLTHANKPFPGLCPVKDAELIAAIREGFRQLPQPAPRHAVAVGS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory